Evaluation of soft tissue regenerative processes after subcutaneous implantation of silver/ poly(vinyl alcohol) and novel silver/poly(vinyl alcohol)/graphene hydrogels in an animal model
Аутори
Lužajić-Božinovski, TijanaTodorovic, Vera
Milosevic, Ivan
Gajdov, Vladimir
Prokić, Bogomir Bolka
Nešović, Katarina
Mišković-Stanković, Vesna
Markovic, Danica
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
A newly produced biomaterial is necessarily subject of standards, which are performed in vivo on animal models. For the evaluation of soft tissue regenerative possibilities after subcutaneous implantation of biomaterials - silver/poly(vinyl alcohol) (Ag/PVA) and novel silver/poly(vinyl alcohol)/graphene (Ag/PVA/Gr) provided for clinical use, sixteen rats were used, according to the instructions of international standards, ISO 10993-6, 2007. Histological sections were observed 7, 15, 30 and 60 days after grafting. These hydrogels were produced by in situ electrochemical synthesis of silver nanoparticles in the polymer matrices, which enabled obtaining completely safe and biocompatible materials, free from any additional toxic chemical reducing agents. Surgical implantation of hydrogels was done according to the permission of the Ethical Committee of the Faculty of Veterinary Medicine, University of Belgrade. Immunohistochemical (IHC) studies included the assessment of smooth muscle expr...ession actin in blood vessels (alpha-SMA), the expression of laminin and type I and type III collagen in the skin structures, and, the determination of cell proliferation marker expression (Ki-67) keratinocytes. The results were assessed in a semiquantitative manner. The data were analyzed in the statistical software package IBM SPSS 20. The conclusions indicated that Ag/PVA/Gr might be used as wound dressings to enhance the tissue healing potential and established faster integration and shorter retention in the tissue.
Кључне речи:
immunohistochemistry / nanocomposite biomaterials / rat / skin regenerationИзвор:
Acta Veterinaria-Beograd, 2021, 71, 3, 285-302Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200143 (Универзитет у Београду, Факултет ветеринарске медицине) (RS-MESTD-inst-2020-200143)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200135 (Универзитет у Београду, Технолошко-металуршки факултет) (RS-MESTD-inst-2020-200135)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200287 (Иновациони центар Технолошко-металуршког факултета у Београду доо) (RS-MESTD-inst-2020-200287)
DOI: 10.2478/acve-2021-0025
ISSN: 0567-8315
WoS: 000703661100004
Scopus: 2-s2.0-85116478653
Институција/група
Tehnološko-metalurški fakultetTY - JOUR AU - Lužajić-Božinovski, Tijana AU - Todorovic, Vera AU - Milosevic, Ivan AU - Gajdov, Vladimir AU - Prokić, Bogomir Bolka AU - Nešović, Katarina AU - Mišković-Stanković, Vesna AU - Markovic, Danica PY - 2021 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4812 AB - A newly produced biomaterial is necessarily subject of standards, which are performed in vivo on animal models. For the evaluation of soft tissue regenerative possibilities after subcutaneous implantation of biomaterials - silver/poly(vinyl alcohol) (Ag/PVA) and novel silver/poly(vinyl alcohol)/graphene (Ag/PVA/Gr) provided for clinical use, sixteen rats were used, according to the instructions of international standards, ISO 10993-6, 2007. Histological sections were observed 7, 15, 30 and 60 days after grafting. These hydrogels were produced by in situ electrochemical synthesis of silver nanoparticles in the polymer matrices, which enabled obtaining completely safe and biocompatible materials, free from any additional toxic chemical reducing agents. Surgical implantation of hydrogels was done according to the permission of the Ethical Committee of the Faculty of Veterinary Medicine, University of Belgrade. Immunohistochemical (IHC) studies included the assessment of smooth muscle expression actin in blood vessels (alpha-SMA), the expression of laminin and type I and type III collagen in the skin structures, and, the determination of cell proliferation marker expression (Ki-67) keratinocytes. The results were assessed in a semiquantitative manner. The data were analyzed in the statistical software package IBM SPSS 20. The conclusions indicated that Ag/PVA/Gr might be used as wound dressings to enhance the tissue healing potential and established faster integration and shorter retention in the tissue. T2 - Acta Veterinaria-Beograd T1 - Evaluation of soft tissue regenerative processes after subcutaneous implantation of silver/ poly(vinyl alcohol) and novel silver/poly(vinyl alcohol)/graphene hydrogels in an animal model EP - 302 IS - 3 SP - 285 VL - 71 DO - 10.2478/acve-2021-0025 ER -
@article{ author = "Lužajić-Božinovski, Tijana and Todorovic, Vera and Milosevic, Ivan and Gajdov, Vladimir and Prokić, Bogomir Bolka and Nešović, Katarina and Mišković-Stanković, Vesna and Markovic, Danica", year = "2021", abstract = "A newly produced biomaterial is necessarily subject of standards, which are performed in vivo on animal models. For the evaluation of soft tissue regenerative possibilities after subcutaneous implantation of biomaterials - silver/poly(vinyl alcohol) (Ag/PVA) and novel silver/poly(vinyl alcohol)/graphene (Ag/PVA/Gr) provided for clinical use, sixteen rats were used, according to the instructions of international standards, ISO 10993-6, 2007. Histological sections were observed 7, 15, 30 and 60 days after grafting. These hydrogels were produced by in situ electrochemical synthesis of silver nanoparticles in the polymer matrices, which enabled obtaining completely safe and biocompatible materials, free from any additional toxic chemical reducing agents. Surgical implantation of hydrogels was done according to the permission of the Ethical Committee of the Faculty of Veterinary Medicine, University of Belgrade. Immunohistochemical (IHC) studies included the assessment of smooth muscle expression actin in blood vessels (alpha-SMA), the expression of laminin and type I and type III collagen in the skin structures, and, the determination of cell proliferation marker expression (Ki-67) keratinocytes. The results were assessed in a semiquantitative manner. The data were analyzed in the statistical software package IBM SPSS 20. The conclusions indicated that Ag/PVA/Gr might be used as wound dressings to enhance the tissue healing potential and established faster integration and shorter retention in the tissue.", journal = "Acta Veterinaria-Beograd", title = "Evaluation of soft tissue regenerative processes after subcutaneous implantation of silver/ poly(vinyl alcohol) and novel silver/poly(vinyl alcohol)/graphene hydrogels in an animal model", pages = "302-285", number = "3", volume = "71", doi = "10.2478/acve-2021-0025" }
Lužajić-Božinovski, T., Todorovic, V., Milosevic, I., Gajdov, V., Prokić, B. B., Nešović, K., Mišković-Stanković, V.,& Markovic, D.. (2021). Evaluation of soft tissue regenerative processes after subcutaneous implantation of silver/ poly(vinyl alcohol) and novel silver/poly(vinyl alcohol)/graphene hydrogels in an animal model. in Acta Veterinaria-Beograd, 71(3), 285-302. https://doi.org/10.2478/acve-2021-0025
Lužajić-Božinovski T, Todorovic V, Milosevic I, Gajdov V, Prokić BB, Nešović K, Mišković-Stanković V, Markovic D. Evaluation of soft tissue regenerative processes after subcutaneous implantation of silver/ poly(vinyl alcohol) and novel silver/poly(vinyl alcohol)/graphene hydrogels in an animal model. in Acta Veterinaria-Beograd. 2021;71(3):285-302. doi:10.2478/acve-2021-0025 .
Lužajić-Božinovski, Tijana, Todorovic, Vera, Milosevic, Ivan, Gajdov, Vladimir, Prokić, Bogomir Bolka, Nešović, Katarina, Mišković-Stanković, Vesna, Markovic, Danica, "Evaluation of soft tissue regenerative processes after subcutaneous implantation of silver/ poly(vinyl alcohol) and novel silver/poly(vinyl alcohol)/graphene hydrogels in an animal model" in Acta Veterinaria-Beograd, 71, no. 3 (2021):285-302, https://doi.org/10.2478/acve-2021-0025 . .