Immobilized Alcalase on Micron- and Submicron-Sized Alginate Beads as a Potential Biocatalyst for Hydrolysis of Food Proteins
Аутори
Jonovic, MarkoZuza, Milena
Đorđević, Verica
Šekuljica, Nataša
Milivojević, Milan
Jugovic, Branimir
Bugarski, Branko
Knežević-Jugović, Zorica
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Enzymatic hydrolysis of food proteins is convenient method to improve their functional properties and physiological activity. Herein, the successful covalent attachment of alcalase on alginate micron and submicron beads using the carbodiimide based chemistry reaction and the subsequent application of the beads for egg white and soy proteins hydrolysis were studied. In addition to the electrostatic extrusion technique (EE) previously used by others, the potential utilization of a novel ultrasonic spray atomization technique without drying (UA) and with drying (UAD) for alginate submicron beads production has been attempted. The immobilization parameters were optimized on microbeads obtained by EE technique (803 +/- 23 mu m) with respect to enzyme loading and alcalase activity. UA and UAD techniques resulted in much smaller particles (607 +/- 103 nm and 394 +/- 51 nm in diameter, respectively), enabling even higher enzyme loading of 671.6 +/- 4 mg g(-1) on the carrier and the highest imm...obilized alcalase activity of 2716.1 IU g(-1) in the standard reaction. The UAD biocatalyst exhibited also better performances in the real food system based on egg white or soy proteins. It has been shown that the immobilized alcalase can be reused in seven successive soy protein hydrolysis cycles with a little decrease in the activity.
Кључне речи:
alcalase / protein hydrolysis / alginate submicron beads / covalent immobilization / electrostatic extrusion / ultrasonic spray atomization techniqueИзвор:
Catalysts, 2021, 11, 3Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200175 (Институт техничких наука САНУ, Београд) (RS-MESTD-inst-2020-200175)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200135 (Универзитет у Београду, Технолошко-металуршки факултет) (RS-MESTD-inst-2020-200135)
DOI: 10.3390/catal11030305
ISSN: 2073-4344
WoS: 000633470000001
Scopus: 2-s2.0-85101520113
Колекције
Институција/група
Tehnološko-metalurški fakultetTY - JOUR AU - Jonovic, Marko AU - Zuza, Milena AU - Đorđević, Verica AU - Šekuljica, Nataša AU - Milivojević, Milan AU - Jugovic, Branimir AU - Bugarski, Branko AU - Knežević-Jugović, Zorica PY - 2021 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4879 AB - Enzymatic hydrolysis of food proteins is convenient method to improve their functional properties and physiological activity. Herein, the successful covalent attachment of alcalase on alginate micron and submicron beads using the carbodiimide based chemistry reaction and the subsequent application of the beads for egg white and soy proteins hydrolysis were studied. In addition to the electrostatic extrusion technique (EE) previously used by others, the potential utilization of a novel ultrasonic spray atomization technique without drying (UA) and with drying (UAD) for alginate submicron beads production has been attempted. The immobilization parameters were optimized on microbeads obtained by EE technique (803 +/- 23 mu m) with respect to enzyme loading and alcalase activity. UA and UAD techniques resulted in much smaller particles (607 +/- 103 nm and 394 +/- 51 nm in diameter, respectively), enabling even higher enzyme loading of 671.6 +/- 4 mg g(-1) on the carrier and the highest immobilized alcalase activity of 2716.1 IU g(-1) in the standard reaction. The UAD biocatalyst exhibited also better performances in the real food system based on egg white or soy proteins. It has been shown that the immobilized alcalase can be reused in seven successive soy protein hydrolysis cycles with a little decrease in the activity. T2 - Catalysts T1 - Immobilized Alcalase on Micron- and Submicron-Sized Alginate Beads as a Potential Biocatalyst for Hydrolysis of Food Proteins IS - 3 VL - 11 DO - 10.3390/catal11030305 ER -
@article{ author = "Jonovic, Marko and Zuza, Milena and Đorđević, Verica and Šekuljica, Nataša and Milivojević, Milan and Jugovic, Branimir and Bugarski, Branko and Knežević-Jugović, Zorica", year = "2021", abstract = "Enzymatic hydrolysis of food proteins is convenient method to improve their functional properties and physiological activity. Herein, the successful covalent attachment of alcalase on alginate micron and submicron beads using the carbodiimide based chemistry reaction and the subsequent application of the beads for egg white and soy proteins hydrolysis were studied. In addition to the electrostatic extrusion technique (EE) previously used by others, the potential utilization of a novel ultrasonic spray atomization technique without drying (UA) and with drying (UAD) for alginate submicron beads production has been attempted. The immobilization parameters were optimized on microbeads obtained by EE technique (803 +/- 23 mu m) with respect to enzyme loading and alcalase activity. UA and UAD techniques resulted in much smaller particles (607 +/- 103 nm and 394 +/- 51 nm in diameter, respectively), enabling even higher enzyme loading of 671.6 +/- 4 mg g(-1) on the carrier and the highest immobilized alcalase activity of 2716.1 IU g(-1) in the standard reaction. The UAD biocatalyst exhibited also better performances in the real food system based on egg white or soy proteins. It has been shown that the immobilized alcalase can be reused in seven successive soy protein hydrolysis cycles with a little decrease in the activity.", journal = "Catalysts", title = "Immobilized Alcalase on Micron- and Submicron-Sized Alginate Beads as a Potential Biocatalyst for Hydrolysis of Food Proteins", number = "3", volume = "11", doi = "10.3390/catal11030305" }
Jonovic, M., Zuza, M., Đorđević, V., Šekuljica, N., Milivojević, M., Jugovic, B., Bugarski, B.,& Knežević-Jugović, Z.. (2021). Immobilized Alcalase on Micron- and Submicron-Sized Alginate Beads as a Potential Biocatalyst for Hydrolysis of Food Proteins. in Catalysts, 11(3). https://doi.org/10.3390/catal11030305
Jonovic M, Zuza M, Đorđević V, Šekuljica N, Milivojević M, Jugovic B, Bugarski B, Knežević-Jugović Z. Immobilized Alcalase on Micron- and Submicron-Sized Alginate Beads as a Potential Biocatalyst for Hydrolysis of Food Proteins. in Catalysts. 2021;11(3). doi:10.3390/catal11030305 .
Jonovic, Marko, Zuza, Milena, Đorđević, Verica, Šekuljica, Nataša, Milivojević, Milan, Jugovic, Branimir, Bugarski, Branko, Knežević-Jugović, Zorica, "Immobilized Alcalase on Micron- and Submicron-Sized Alginate Beads as a Potential Biocatalyst for Hydrolysis of Food Proteins" in Catalysts, 11, no. 3 (2021), https://doi.org/10.3390/catal11030305 . .