Lipase from Candida rugosa was immobilized in alginate beads for possible application in non-aqueous or microaqueous reaction systems. An electrostatic droplet generation technique was used for production of small diameter ( lt 1 mm) lipase-alginate beads. This technique provided negligible loss of the lipase (immobilization efficiencies were 98.2-99.2%). Under optimal immobilization conditions (applied potential 4.9 kV, needle gauge 21, 2% sodium alginate solution) the lipase-alginate beads, 0.65 mm in diameter, retained enzyme activity equivalent to 75% that of free lipase. The activity of the immobilized lipase was verified in the reaction of palm oil hydrolysis in a lecithin/isooctane system. The reaction rate with alginate-immobilized lipase was lower than with the free enzyme but the final conversions were approximately the same (similar to 74%). Immobilized lipase could be used for up to three reaction cycles with little loss of activity.
TY - JOUR
AU - Knežević, Zorica
AU - Bobić, Svetlana
AU - Milutinović, A
AU - Obradović, Bojana
AU - Mojović, Ljiljana
AU - Bugarski, Branko
PY - 2002
UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/493
AB - Lipase from Candida rugosa was immobilized in alginate beads for possible application in non-aqueous or microaqueous reaction systems. An electrostatic droplet generation technique was used for production of small diameter ( lt 1 mm) lipase-alginate beads. This technique provided negligible loss of the lipase (immobilization efficiencies were 98.2-99.2%). Under optimal immobilization conditions (applied potential 4.9 kV, needle gauge 21, 2% sodium alginate solution) the lipase-alginate beads, 0.65 mm in diameter, retained enzyme activity equivalent to 75% that of free lipase. The activity of the immobilized lipase was verified in the reaction of palm oil hydrolysis in a lecithin/isooctane system. The reaction rate with alginate-immobilized lipase was lower than with the free enzyme but the final conversions were approximately the same (similar to 74%). Immobilized lipase could be used for up to three reaction cycles with little loss of activity.
PB - Elsevier Sci Ltd, Oxford
T2 - Process Biochemistry
T1 - Alginate-immobilized lipase by electrostatic extrusion for the purpose of palm oil hydrolysis in lecithin/isooctane system
EP - 318
IS - 3
SP - 313
VL - 38
DO - 10.1016/S0032-9592(02)00085-7
ER -
@article{
author = "Knežević, Zorica and Bobić, Svetlana and Milutinović, A and Obradović, Bojana and Mojović, Ljiljana and Bugarski, Branko",
year = "2002",
abstract = "Lipase from Candida rugosa was immobilized in alginate beads for possible application in non-aqueous or microaqueous reaction systems. An electrostatic droplet generation technique was used for production of small diameter ( lt 1 mm) lipase-alginate beads. This technique provided negligible loss of the lipase (immobilization efficiencies were 98.2-99.2%). Under optimal immobilization conditions (applied potential 4.9 kV, needle gauge 21, 2% sodium alginate solution) the lipase-alginate beads, 0.65 mm in diameter, retained enzyme activity equivalent to 75% that of free lipase. The activity of the immobilized lipase was verified in the reaction of palm oil hydrolysis in a lecithin/isooctane system. The reaction rate with alginate-immobilized lipase was lower than with the free enzyme but the final conversions were approximately the same (similar to 74%). Immobilized lipase could be used for up to three reaction cycles with little loss of activity.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Process Biochemistry",
title = "Alginate-immobilized lipase by electrostatic extrusion for the purpose of palm oil hydrolysis in lecithin/isooctane system",
pages = "318-313",
number = "3",
volume = "38",
doi = "10.1016/S0032-9592(02)00085-7"
}
Knežević, Z., Bobić, S., Milutinović, A., Obradović, B., Mojović, L.,& Bugarski, B.. (2002). Alginate-immobilized lipase by electrostatic extrusion for the purpose of palm oil hydrolysis in lecithin/isooctane system. in Process Biochemistry
Elsevier Sci Ltd, Oxford., 38(3), 313-318.
https://doi.org/10.1016/S0032-9592(02)00085-7
Knežević Z, Bobić S, Milutinović A, Obradović B, Mojović L, Bugarski B. Alginate-immobilized lipase by electrostatic extrusion for the purpose of palm oil hydrolysis in lecithin/isooctane system. in Process Biochemistry. 2002;38(3):313-318.
doi:10.1016/S0032-9592(02)00085-7 .
Knežević, Zorica, Bobić, Svetlana, Milutinović, A, Obradović, Bojana, Mojović, Ljiljana, Bugarski, Branko, "Alginate-immobilized lipase by electrostatic extrusion for the purpose of palm oil hydrolysis in lecithin/isooctane system" in Process Biochemistry, 38, no. 3 (2002):313-318,
https://doi.org/10.1016/S0032-9592(02)00085-7 . .
