The development of a combined enzymatic and microbial fermentation as a viable technology for the spent coffee ground full utilization
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2023
Authors
Milić, MarijaBuntić, Aneta V.
Mihajlovski, Katarina
Ilić, Nevena
Davidović, Slađana
Dimitrijević-Branković, Suzana
Article (Published version)
Metadata
Show full item recordAbstract
This study demonstrated the innovative biotransformation aspect of spent coffee grounds (SCG) by application of the specific enzymatic cocktail with addition of lactic acid bacteria strain Lactobacillus rhamnosus (ATCC (R) 7469 (TM)). This bioprocess induced the improvement of the most of the quality parameters in SCG, causing a considerable increase in the bioactive contents (total polyphenols, chlorogenic acid, reducing sugars, free amino acids) and antioxidant activity, in comparison to the non-fermented SCG. Under the optimal conditions, at 37 degrees C, for 24 h, with 5% v/w of the inoculum employed, the polyphenols were increased for 67% (227.3 +/- 3.3 mg GAE/g dm extract), reducing sugars 57% (277.9 +/- 7.6 mg glucose/g dm extract), and alpha-amino nitrogen 80% (161.2 +/- 9.8 mg/100 g dm extract), and the chlorogenic acid content was doubled, while the caffeine was reduced for 38%. Furthermore, the resulting product evinced a strong antioxidant activity detected via inhibition o...f DPPH radicals and reducing capacity assay (FRAP). The proposed technology is viable for a large-scale commercialization for utilization of easily accessible industrial residues, deriving a product with a nutritive formulation very desirable to be incorporated as an additive in food and feed industry.
Keywords:
Spent coffee grounds / Fermentation / Polyphenols / Antioxidants / Enzymes / Lactic acid bacteriaSource:
Biomass Conversion and Biorefinery, 2023, 13, 8, 6747-6759Funding / projects:
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200011 (Institute of Soil Sciences, Belgrade) (RS-200011)
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200135 (University of Belgrade, Faculty of Technology and Metallurgy) (RS-200135)
DOI: 10.1007/s13399-021-01605-8
ISSN: 2190-6815
WoS: 000657221000002
Scopus: 2-s2.0-85107393300
Institution/Community
Tehnološko-metalurški fakultetTY - JOUR AU - Milić, Marija AU - Buntić, Aneta V. AU - Mihajlovski, Katarina AU - Ilić, Nevena AU - Davidović, Slađana AU - Dimitrijević-Branković, Suzana PY - 2023 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4981 AB - This study demonstrated the innovative biotransformation aspect of spent coffee grounds (SCG) by application of the specific enzymatic cocktail with addition of lactic acid bacteria strain Lactobacillus rhamnosus (ATCC (R) 7469 (TM)). This bioprocess induced the improvement of the most of the quality parameters in SCG, causing a considerable increase in the bioactive contents (total polyphenols, chlorogenic acid, reducing sugars, free amino acids) and antioxidant activity, in comparison to the non-fermented SCG. Under the optimal conditions, at 37 degrees C, for 24 h, with 5% v/w of the inoculum employed, the polyphenols were increased for 67% (227.3 +/- 3.3 mg GAE/g dm extract), reducing sugars 57% (277.9 +/- 7.6 mg glucose/g dm extract), and alpha-amino nitrogen 80% (161.2 +/- 9.8 mg/100 g dm extract), and the chlorogenic acid content was doubled, while the caffeine was reduced for 38%. Furthermore, the resulting product evinced a strong antioxidant activity detected via inhibition of DPPH radicals and reducing capacity assay (FRAP). The proposed technology is viable for a large-scale commercialization for utilization of easily accessible industrial residues, deriving a product with a nutritive formulation very desirable to be incorporated as an additive in food and feed industry. T2 - Biomass Conversion and Biorefinery T1 - The development of a combined enzymatic and microbial fermentation as a viable technology for the spent coffee ground full utilization EP - 6759 IS - 8 SP - 6747 VL - 13 DO - 10.1007/s13399-021-01605-8 ER -
@article{ author = "Milić, Marija and Buntić, Aneta V. and Mihajlovski, Katarina and Ilić, Nevena and Davidović, Slađana and Dimitrijević-Branković, Suzana", year = "2023", abstract = "This study demonstrated the innovative biotransformation aspect of spent coffee grounds (SCG) by application of the specific enzymatic cocktail with addition of lactic acid bacteria strain Lactobacillus rhamnosus (ATCC (R) 7469 (TM)). This bioprocess induced the improvement of the most of the quality parameters in SCG, causing a considerable increase in the bioactive contents (total polyphenols, chlorogenic acid, reducing sugars, free amino acids) and antioxidant activity, in comparison to the non-fermented SCG. Under the optimal conditions, at 37 degrees C, for 24 h, with 5% v/w of the inoculum employed, the polyphenols were increased for 67% (227.3 +/- 3.3 mg GAE/g dm extract), reducing sugars 57% (277.9 +/- 7.6 mg glucose/g dm extract), and alpha-amino nitrogen 80% (161.2 +/- 9.8 mg/100 g dm extract), and the chlorogenic acid content was doubled, while the caffeine was reduced for 38%. Furthermore, the resulting product evinced a strong antioxidant activity detected via inhibition of DPPH radicals and reducing capacity assay (FRAP). The proposed technology is viable for a large-scale commercialization for utilization of easily accessible industrial residues, deriving a product with a nutritive formulation very desirable to be incorporated as an additive in food and feed industry.", journal = "Biomass Conversion and Biorefinery", title = "The development of a combined enzymatic and microbial fermentation as a viable technology for the spent coffee ground full utilization", pages = "6759-6747", number = "8", volume = "13", doi = "10.1007/s13399-021-01605-8" }
Milić, M., Buntić, A. V., Mihajlovski, K., Ilić, N., Davidović, S.,& Dimitrijević-Branković, S.. (2023). The development of a combined enzymatic and microbial fermentation as a viable technology for the spent coffee ground full utilization. in Biomass Conversion and Biorefinery, 13(8), 6747-6759. https://doi.org/10.1007/s13399-021-01605-8
Milić M, Buntić AV, Mihajlovski K, Ilić N, Davidović S, Dimitrijević-Branković S. The development of a combined enzymatic and microbial fermentation as a viable technology for the spent coffee ground full utilization. in Biomass Conversion and Biorefinery. 2023;13(8):6747-6759. doi:10.1007/s13399-021-01605-8 .
Milić, Marija, Buntić, Aneta V., Mihajlovski, Katarina, Ilić, Nevena, Davidović, Slađana, Dimitrijević-Branković, Suzana, "The development of a combined enzymatic and microbial fermentation as a viable technology for the spent coffee ground full utilization" in Biomass Conversion and Biorefinery, 13, no. 8 (2023):6747-6759, https://doi.org/10.1007/s13399-021-01605-8 . .