Strong and tough, pH sensible, interpenetrating network hydrogels based on gelatin and poly(methacrylic acid)
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2022
Authors
Ugrinović, VukašinPanić, Vesna
Spasojević, Pavle
Seslija, Sanja
Bozic, Bojan
Petrović, Rada
Janaćković, Đorđe
Veljović, Đorđe
Article (Published version)
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Hydrogels are promising materials for biomedical applications due to highly hydrated, porous, permeable structure with possibility to accommodate living cells, drugs, or bioactive factors. In this paper, we reported poly(methacrylic acid) (PMAA)/gelatin IPN hydrogels, synthesized by free-radical polymerization, with adjustable mechanical, structural, physicochemical, and biological characteristics. The influence of methacrylic acid (MAA), gelatin, and cross-linker in the precursor solution on hydrogels properties was investigated. The increasing concentration of MAA, gelatin, and cross-linker led to better mechanical properties, lower porosity, and water content. The compressive mechanical properties of hydrogels were significantly better in comparison to a single-network PMAA hydrogel, while the obtained compressive strength values up to 16 MPa were comparable with tough hydrogels. The increasing concentration of MAA and cross-linker reduced fatigue resistance and degradability, while... the increase in gelatin content acted in the opposite way. Swelling tests in different pH conditions demonstrated strong pH-sensibility of the hydrogels, which was more pronounced as MAA concentration was higher, and gelatin and cross-linker concentrations were lower. In addition, the hydrogels strongly promoted the proliferation of human periodontal ligament stem cells and MRC-5 cells as assayed by MTT assay.
Keywords:
gelatin / IPN hydrogel / poly(methacrylic acid) / tissue engineeringSource:
Polymer Engineering and Science, 2022Funding / projects:
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200026 (University of Belgrade, Institute of Chemistry, Technology and Metallurgy - IChTM) (RS-MESTD-inst-2020-200026)
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200135 (University of Belgrade, Faculty of Technology and Metallurgy) (RS-MESTD-inst-2020-200135)
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200178 (University of Belgrade, Faculty of Biology) (RS-MESTD-inst-2020-200178)
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200287 (Innovation Center of the Faculty of Technology and Metallurgy) (RS-MESTD-inst-2020-200287)
DOI: 10.1002/pen.25870
ISSN: 0032-3888
WoS: 000734793000001
Scopus: 2-s2.0-85122011824
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Inovacioni centarTY - JOUR AU - Ugrinović, Vukašin AU - Panić, Vesna AU - Spasojević, Pavle AU - Seslija, Sanja AU - Bozic, Bojan AU - Petrović, Rada AU - Janaćković, Đorđe AU - Veljović, Đorđe PY - 2022 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4984 AB - Hydrogels are promising materials for biomedical applications due to highly hydrated, porous, permeable structure with possibility to accommodate living cells, drugs, or bioactive factors. In this paper, we reported poly(methacrylic acid) (PMAA)/gelatin IPN hydrogels, synthesized by free-radical polymerization, with adjustable mechanical, structural, physicochemical, and biological characteristics. The influence of methacrylic acid (MAA), gelatin, and cross-linker in the precursor solution on hydrogels properties was investigated. The increasing concentration of MAA, gelatin, and cross-linker led to better mechanical properties, lower porosity, and water content. The compressive mechanical properties of hydrogels were significantly better in comparison to a single-network PMAA hydrogel, while the obtained compressive strength values up to 16 MPa were comparable with tough hydrogels. The increasing concentration of MAA and cross-linker reduced fatigue resistance and degradability, while the increase in gelatin content acted in the opposite way. Swelling tests in different pH conditions demonstrated strong pH-sensibility of the hydrogels, which was more pronounced as MAA concentration was higher, and gelatin and cross-linker concentrations were lower. In addition, the hydrogels strongly promoted the proliferation of human periodontal ligament stem cells and MRC-5 cells as assayed by MTT assay. T2 - Polymer Engineering and Science T1 - Strong and tough, pH sensible, interpenetrating network hydrogels based on gelatin and poly(methacrylic acid) DO - 10.1002/pen.25870 ER -
@article{ author = "Ugrinović, Vukašin and Panić, Vesna and Spasojević, Pavle and Seslija, Sanja and Bozic, Bojan and Petrović, Rada and Janaćković, Đorđe and Veljović, Đorđe", year = "2022", abstract = "Hydrogels are promising materials for biomedical applications due to highly hydrated, porous, permeable structure with possibility to accommodate living cells, drugs, or bioactive factors. In this paper, we reported poly(methacrylic acid) (PMAA)/gelatin IPN hydrogels, synthesized by free-radical polymerization, with adjustable mechanical, structural, physicochemical, and biological characteristics. The influence of methacrylic acid (MAA), gelatin, and cross-linker in the precursor solution on hydrogels properties was investigated. The increasing concentration of MAA, gelatin, and cross-linker led to better mechanical properties, lower porosity, and water content. The compressive mechanical properties of hydrogels were significantly better in comparison to a single-network PMAA hydrogel, while the obtained compressive strength values up to 16 MPa were comparable with tough hydrogels. The increasing concentration of MAA and cross-linker reduced fatigue resistance and degradability, while the increase in gelatin content acted in the opposite way. Swelling tests in different pH conditions demonstrated strong pH-sensibility of the hydrogels, which was more pronounced as MAA concentration was higher, and gelatin and cross-linker concentrations were lower. In addition, the hydrogels strongly promoted the proliferation of human periodontal ligament stem cells and MRC-5 cells as assayed by MTT assay.", journal = "Polymer Engineering and Science", title = "Strong and tough, pH sensible, interpenetrating network hydrogels based on gelatin and poly(methacrylic acid)", doi = "10.1002/pen.25870" }
Ugrinović, V., Panić, V., Spasojević, P., Seslija, S., Bozic, B., Petrović, R., Janaćković, Đ.,& Veljović, Đ.. (2022). Strong and tough, pH sensible, interpenetrating network hydrogels based on gelatin and poly(methacrylic acid). in Polymer Engineering and Science. https://doi.org/10.1002/pen.25870
Ugrinović V, Panić V, Spasojević P, Seslija S, Bozic B, Petrović R, Janaćković Đ, Veljović Đ. Strong and tough, pH sensible, interpenetrating network hydrogels based on gelatin and poly(methacrylic acid). in Polymer Engineering and Science. 2022;. doi:10.1002/pen.25870 .
Ugrinović, Vukašin, Panić, Vesna, Spasojević, Pavle, Seslija, Sanja, Bozic, Bojan, Petrović, Rada, Janaćković, Đorđe, Veljović, Đorđe, "Strong and tough, pH sensible, interpenetrating network hydrogels based on gelatin and poly(methacrylic acid)" in Polymer Engineering and Science (2022), https://doi.org/10.1002/pen.25870 . .