Chromatophores isolated from the Siamese fighting fish, Betta splendens, represent a class of living cells that provide a vivid color response to microbial pathogens and environmental toxins. The selection of the most appropriate microcarrier and the development of the optimal technique for the chromatophore immobilization in order to enable directed transport of the sensor cells throughout microchannels of the biosensor, as well to preserve the cell survival and its functionality was studied. Microcarriers derived from glass, polystyrene and gelatin (collagen) were tested as substrates for chromatophore attachement. Gelatin microcarriers were found to be the most suitable, due to high attachment efficiency (95% of attached cells), preservation of the cell viability and enhanced cell sensitivity. The optimum conditions for fish cell immobilization on collagen microcarriers were determined based on the cell-to-microcarrier bead ratio and the pH of the solution. The rate of cell attachme...nt to the gelatin microcarrier followed first-order kinetics. Pretreatment of the gelatin beads with fibronectin, known as a cell attachment-promoting agent, resulted in a 10% higher attachment rate constant (k).
TY - JOUR
AU - Mojović, Ljiljana
AU - Jovanović, G.
PY - 2003
UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/502
AB - Chromatophores isolated from the Siamese fighting fish, Betta splendens, represent a class of living cells that provide a vivid color response to microbial pathogens and environmental toxins. The selection of the most appropriate microcarrier and the development of the optimal technique for the chromatophore immobilization in order to enable directed transport of the sensor cells throughout microchannels of the biosensor, as well to preserve the cell survival and its functionality was studied. Microcarriers derived from glass, polystyrene and gelatin (collagen) were tested as substrates for chromatophore attachement. Gelatin microcarriers were found to be the most suitable, due to high attachment efficiency (95% of attached cells), preservation of the cell viability and enhanced cell sensitivity. The optimum conditions for fish cell immobilization on collagen microcarriers were determined based on the cell-to-microcarrier bead ratio and the pH of the solution. The rate of cell attachment to the gelatin microcarrier followed first-order kinetics. Pretreatment of the gelatin beads with fibronectin, known as a cell attachment-promoting agent, resulted in a 10% higher attachment rate constant (k).
PB - Association of the Chemical Engineers of Serbia
T2 - Chemical Industry & Chemical Engineering Quarterly
T1 - Immobilization of fish chromatophores for use as a micro-biosensor for biological toxins
T1 - Imobilizacija hromatofora riba radi korišćenja kao biosenzor za biološke toksine
EP - 610
IS - 4
SP - 605
VL - 9
UR - https://hdl.handle.net/21.15107/rcub_technorep_502
ER -
@article{
author = "Mojović, Ljiljana and Jovanović, G.",
year = "2003",
abstract = "Chromatophores isolated from the Siamese fighting fish, Betta splendens, represent a class of living cells that provide a vivid color response to microbial pathogens and environmental toxins. The selection of the most appropriate microcarrier and the development of the optimal technique for the chromatophore immobilization in order to enable directed transport of the sensor cells throughout microchannels of the biosensor, as well to preserve the cell survival and its functionality was studied. Microcarriers derived from glass, polystyrene and gelatin (collagen) were tested as substrates for chromatophore attachement. Gelatin microcarriers were found to be the most suitable, due to high attachment efficiency (95% of attached cells), preservation of the cell viability and enhanced cell sensitivity. The optimum conditions for fish cell immobilization on collagen microcarriers were determined based on the cell-to-microcarrier bead ratio and the pH of the solution. The rate of cell attachment to the gelatin microcarrier followed first-order kinetics. Pretreatment of the gelatin beads with fibronectin, known as a cell attachment-promoting agent, resulted in a 10% higher attachment rate constant (k).",
publisher = "Association of the Chemical Engineers of Serbia",
journal = "Chemical Industry & Chemical Engineering Quarterly",
title = "Immobilization of fish chromatophores for use as a micro-biosensor for biological toxins, Imobilizacija hromatofora riba radi korišćenja kao biosenzor za biološke toksine",
pages = "610-605",
number = "4",
volume = "9",
url = "https://hdl.handle.net/21.15107/rcub_technorep_502"
}
Mojović, L.,& Jovanović, G.. (2003). Immobilization of fish chromatophores for use as a micro-biosensor for biological toxins. in Chemical Industry & Chemical Engineering Quarterly
Association of the Chemical Engineers of Serbia., 9(4), 605-610.
https://hdl.handle.net/21.15107/rcub_technorep_502
Mojović L, Jovanović G. Immobilization of fish chromatophores for use as a micro-biosensor for biological toxins. in Chemical Industry & Chemical Engineering Quarterly. 2003;9(4):605-610.
https://hdl.handle.net/21.15107/rcub_technorep_502 .
Mojović, Ljiljana, Jovanović, G., "Immobilization of fish chromatophores for use as a micro-biosensor for biological toxins" in Chemical Industry & Chemical Engineering Quarterly, 9, no. 4 (2003):605-610,
https://hdl.handle.net/21.15107/rcub_technorep_502 .
