Optimization of Pretreatment Conditions and Enzymatic Hydrolysis of Corn Cobs for Production of Microbial Lipids by Trichosporon oleaginosus
Authors
Grubišić, MarinaPerečinec Galić, Maja
Peremin, Ines
Mihajlovski, Katarina
Beluhan, Sunčica
Šantek, Božidar
Šantek Ivančić, Mirela
Article (Published version)
Metadata
Show full item recordAbstract
Microbial lipids produced from lignocellulosic biomass are sustainable alternative feedstock for biodiesel production. In this study, corn cobs were used as a carbon source for lipid production and growth of oleaginous yeast Trichosporon oleaginosus. Lignocellulosic biomass was subjected to alkali and acid pretreatment using sulfuric acid and sodium hydroxide under different temperatures, catalyst concentrations and treatment times. Pretreatment of corn cobs was followed by cellulase hydrolysis. Hydrolysis of alkali pretreated (2% NaOH at 50◦C for 6 h, 1% NaOH at 50◦C for 16 h, 2% NaOH at 121◦C for 1 h, 1% NaOH at 121◦C for 2 h) and acid pretreated (1% H2SO4 120◦C for 20 min, and 2% H2SO4 120◦C for 10 min) corn cobs resulted in more than 80% of the theoretical yield of glucose. The effect of substrate (5, 10, 15 and 20%, g g−1 ) and cellulase loading (15 and 30 Filter Paper Units per gram of glucan, FPU g−1 ) on fermentable sugar yield was also studied. The maximal glucose concentratio...n of 81.64 g L−1 was obtained from alkali-pretreated corn cobs (2% NaOH at 50◦C for 6 h) at 20% substrate loading and 30 FPU of Cellic CTec2 g−1 of glucan. Enzymatic hydrolysates of pretreated biomasses and filtrates of lignocellulosic slurries obtained after pretreatment were used for growth and lipid synthesis by T. oleaginosus. The highest lipid concentration of 18.97 g L−1 was obtained on hydrolysate of alkali-pretreated corn cobs (with 1% NaOH at 50◦C for 16 h) using a 15% (g g−1 ) substrate loading and 15 FPU g−1 of cellulase loading. Significant lipid accumulation was also achieved using undetoxified filtrates of pretreated slurries as substrates. Results showed that pretreated corn cobs and undetoxified filtrates are suitable carbon sources for the growth and efficient accumulation of lipids in T. oleaginosus.
Keywords:
biodiesel / fatty acids / lignocellulose / microbial lipids / oleaginous yeasts / pretreatmentSource:
Energies, 2022, 15, 9, 3208-Publisher:
- MDPI
Funding / projects:
- This research received was funded by the Croatian Science Foundation under the project “Sustainable production of biochemicals from waste lignocellulose containing feedstocks” (Croatian Science Foundation no. 9717).
DOI: 10.3390/en15093208
ISSN: 1996-1073
WoS: 00079465170000
Scopus: 2-s2.0-85129716025
Institution/Community
Tehnološko-metalurški fakultetTY - JOUR AU - Grubišić, Marina AU - Perečinec Galić, Maja AU - Peremin, Ines AU - Mihajlovski, Katarina AU - Beluhan, Sunčica AU - Šantek, Božidar AU - Šantek Ivančić, Mirela PY - 2022 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5128 AB - Microbial lipids produced from lignocellulosic biomass are sustainable alternative feedstock for biodiesel production. In this study, corn cobs were used as a carbon source for lipid production and growth of oleaginous yeast Trichosporon oleaginosus. Lignocellulosic biomass was subjected to alkali and acid pretreatment using sulfuric acid and sodium hydroxide under different temperatures, catalyst concentrations and treatment times. Pretreatment of corn cobs was followed by cellulase hydrolysis. Hydrolysis of alkali pretreated (2% NaOH at 50◦C for 6 h, 1% NaOH at 50◦C for 16 h, 2% NaOH at 121◦C for 1 h, 1% NaOH at 121◦C for 2 h) and acid pretreated (1% H2SO4 120◦C for 20 min, and 2% H2SO4 120◦C for 10 min) corn cobs resulted in more than 80% of the theoretical yield of glucose. The effect of substrate (5, 10, 15 and 20%, g g−1 ) and cellulase loading (15 and 30 Filter Paper Units per gram of glucan, FPU g−1 ) on fermentable sugar yield was also studied. The maximal glucose concentration of 81.64 g L−1 was obtained from alkali-pretreated corn cobs (2% NaOH at 50◦C for 6 h) at 20% substrate loading and 30 FPU of Cellic CTec2 g−1 of glucan. Enzymatic hydrolysates of pretreated biomasses and filtrates of lignocellulosic slurries obtained after pretreatment were used for growth and lipid synthesis by T. oleaginosus. The highest lipid concentration of 18.97 g L−1 was obtained on hydrolysate of alkali-pretreated corn cobs (with 1% NaOH at 50◦C for 16 h) using a 15% (g g−1 ) substrate loading and 15 FPU g−1 of cellulase loading. Significant lipid accumulation was also achieved using undetoxified filtrates of pretreated slurries as substrates. Results showed that pretreated corn cobs and undetoxified filtrates are suitable carbon sources for the growth and efficient accumulation of lipids in T. oleaginosus. PB - MDPI T2 - Energies T1 - Optimization of Pretreatment Conditions and Enzymatic Hydrolysis of Corn Cobs for Production of Microbial Lipids by Trichosporon oleaginosus IS - 9 SP - 3208 VL - 15 DO - 10.3390/en15093208 ER -
@article{ author = "Grubišić, Marina and Perečinec Galić, Maja and Peremin, Ines and Mihajlovski, Katarina and Beluhan, Sunčica and Šantek, Božidar and Šantek Ivančić, Mirela", year = "2022", abstract = "Microbial lipids produced from lignocellulosic biomass are sustainable alternative feedstock for biodiesel production. In this study, corn cobs were used as a carbon source for lipid production and growth of oleaginous yeast Trichosporon oleaginosus. Lignocellulosic biomass was subjected to alkali and acid pretreatment using sulfuric acid and sodium hydroxide under different temperatures, catalyst concentrations and treatment times. Pretreatment of corn cobs was followed by cellulase hydrolysis. Hydrolysis of alkali pretreated (2% NaOH at 50◦C for 6 h, 1% NaOH at 50◦C for 16 h, 2% NaOH at 121◦C for 1 h, 1% NaOH at 121◦C for 2 h) and acid pretreated (1% H2SO4 120◦C for 20 min, and 2% H2SO4 120◦C for 10 min) corn cobs resulted in more than 80% of the theoretical yield of glucose. The effect of substrate (5, 10, 15 and 20%, g g−1 ) and cellulase loading (15 and 30 Filter Paper Units per gram of glucan, FPU g−1 ) on fermentable sugar yield was also studied. The maximal glucose concentration of 81.64 g L−1 was obtained from alkali-pretreated corn cobs (2% NaOH at 50◦C for 6 h) at 20% substrate loading and 30 FPU of Cellic CTec2 g−1 of glucan. Enzymatic hydrolysates of pretreated biomasses and filtrates of lignocellulosic slurries obtained after pretreatment were used for growth and lipid synthesis by T. oleaginosus. The highest lipid concentration of 18.97 g L−1 was obtained on hydrolysate of alkali-pretreated corn cobs (with 1% NaOH at 50◦C for 16 h) using a 15% (g g−1 ) substrate loading and 15 FPU g−1 of cellulase loading. Significant lipid accumulation was also achieved using undetoxified filtrates of pretreated slurries as substrates. Results showed that pretreated corn cobs and undetoxified filtrates are suitable carbon sources for the growth and efficient accumulation of lipids in T. oleaginosus.", publisher = "MDPI", journal = "Energies", title = "Optimization of Pretreatment Conditions and Enzymatic Hydrolysis of Corn Cobs for Production of Microbial Lipids by Trichosporon oleaginosus", number = "9", pages = "3208", volume = "15", doi = "10.3390/en15093208" }
Grubišić, M., Perečinec Galić, M., Peremin, I., Mihajlovski, K., Beluhan, S., Šantek, B.,& Šantek Ivančić, M.. (2022). Optimization of Pretreatment Conditions and Enzymatic Hydrolysis of Corn Cobs for Production of Microbial Lipids by Trichosporon oleaginosus. in Energies MDPI., 15(9), 3208. https://doi.org/10.3390/en15093208
Grubišić M, Perečinec Galić M, Peremin I, Mihajlovski K, Beluhan S, Šantek B, Šantek Ivančić M. Optimization of Pretreatment Conditions and Enzymatic Hydrolysis of Corn Cobs for Production of Microbial Lipids by Trichosporon oleaginosus. in Energies. 2022;15(9):3208. doi:10.3390/en15093208 .
Grubišić, Marina, Perečinec Galić, Maja, Peremin, Ines, Mihajlovski, Katarina, Beluhan, Sunčica, Šantek, Božidar, Šantek Ivančić, Mirela, "Optimization of Pretreatment Conditions and Enzymatic Hydrolysis of Corn Cobs for Production of Microbial Lipids by Trichosporon oleaginosus" in Energies, 15, no. 9 (2022):3208, https://doi.org/10.3390/en15093208 . .