Prebiotic effect of galacto-oligosaccharides on the skin microbiota and determination of their diffusion properties
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2022
Authors
Petrov, AnjaĆorović, Marija
Milivojević, Ana
Simović, Milica
Banjanac, Katarina
Pjanović, Rada
Bezbradica, Dejan
Article (Published version)
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Recently, prebiotics are attracting plenty of attention in the field of skin care, since it is found that they are able to support the balance of beneficial and harmful microorganisms on the skin, and accordingly prevent several skin conditions associated with microbial imbalance. Topical application of prebiotics, although insufficiently investigated, holds great promise in improving skin health. The purpose of this research was to determine the prebiotic potential of galacto-oligosaccharides (GOS) for skin microbiota and suitability for incorporation in different topical formulations, and finally, provide insights into their diffusion properties. Methods: The prebiotic effect of GOS was evaluated through the influence on the growth of Staphylococcus epidermidis and Staphylococcus aureus, the most common resident and pathogenic bacterium of the skin microbiota, respectively. Also, with the future use of GOS in cosmetic products in mind, the diffusion of GOS molecules from two differen...t topical formulations, hydrogel and oil-in-water (O/W) gel emulsion, was monitored employing Franz diffusion cell and two systems—with cellulose acetate membrane and transdermal diffusion test model, Strat-M® membrane. Course of fermentation and the amount of diffused GOS molecules were monitored using high-performance liquid chromatography (HPLC). Results: The in vitro results revealed that GOS at a concentration of 5% (w/v) has a pronounced stimulatory effect on S. epidermidis, while simultaneously showing an inhibitory effect on S. aureus, both in nutrient broth and cosmetic formulations. GOS trisaccharide and tetrasaccharide diffusion coefficients from O/W gel emulsion were calculated to be 5.61·10−6 cm2 s−1 and 1.41·10−8 cm2 s−1, respectively. The diffusion coefficient of GOS trisaccharides from hydrogel was 3.22·10−6 cm2 s−1, while it was not determined for tetrasaccharides due to low diffused concentration. Transdermal diffusion tests revealed that GOS incorporated in two formulations stays at the surface of the skin even after 24 h. Conclusion: When applied in adequate concentration, GOS has the potential to be used as a skin prebiotic. Novel GOS enriched formulations, Aristoflex® AVC-based hydrogel and Heliogel™-based O/W gel emulsion, provided efficient diffusion and delivery of prebiotic GOS molecules to the skin surface.
Keywords:
diffusion study / galacto-oligosaccharides / microbiology / skin prebiotic / sustained delivery / topical formulationSource:
International Journal of Cosmetic Science, 2022, 44, 3, 309-319Publisher:
- John Wiley and Sons Inc.
Funding / projects:
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200135 (University of Belgrade, Faculty of Technology and Metallurgy) (RS-200135)
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200287 (Innovation Center of the Faculty of Technology and Metallurgy) (RS-200287)
DOI: 10.1111/ics.12778
ISSN: 0142-5463
WoS: 00079435810000
Scopus: 2-s2.0-85129839867
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Inovacioni centarTY - JOUR AU - Petrov, Anja AU - Ćorović, Marija AU - Milivojević, Ana AU - Simović, Milica AU - Banjanac, Katarina AU - Pjanović, Rada AU - Bezbradica, Dejan PY - 2022 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5141 AB - Recently, prebiotics are attracting plenty of attention in the field of skin care, since it is found that they are able to support the balance of beneficial and harmful microorganisms on the skin, and accordingly prevent several skin conditions associated with microbial imbalance. Topical application of prebiotics, although insufficiently investigated, holds great promise in improving skin health. The purpose of this research was to determine the prebiotic potential of galacto-oligosaccharides (GOS) for skin microbiota and suitability for incorporation in different topical formulations, and finally, provide insights into their diffusion properties. Methods: The prebiotic effect of GOS was evaluated through the influence on the growth of Staphylococcus epidermidis and Staphylococcus aureus, the most common resident and pathogenic bacterium of the skin microbiota, respectively. Also, with the future use of GOS in cosmetic products in mind, the diffusion of GOS molecules from two different topical formulations, hydrogel and oil-in-water (O/W) gel emulsion, was monitored employing Franz diffusion cell and two systems—with cellulose acetate membrane and transdermal diffusion test model, Strat-M® membrane. Course of fermentation and the amount of diffused GOS molecules were monitored using high-performance liquid chromatography (HPLC). Results: The in vitro results revealed that GOS at a concentration of 5% (w/v) has a pronounced stimulatory effect on S. epidermidis, while simultaneously showing an inhibitory effect on S. aureus, both in nutrient broth and cosmetic formulations. GOS trisaccharide and tetrasaccharide diffusion coefficients from O/W gel emulsion were calculated to be 5.61·10−6 cm2 s−1 and 1.41·10−8 cm2 s−1, respectively. The diffusion coefficient of GOS trisaccharides from hydrogel was 3.22·10−6 cm2 s−1, while it was not determined for tetrasaccharides due to low diffused concentration. Transdermal diffusion tests revealed that GOS incorporated in two formulations stays at the surface of the skin even after 24 h. Conclusion: When applied in adequate concentration, GOS has the potential to be used as a skin prebiotic. Novel GOS enriched formulations, Aristoflex® AVC-based hydrogel and Heliogel™-based O/W gel emulsion, provided efficient diffusion and delivery of prebiotic GOS molecules to the skin surface. PB - John Wiley and Sons Inc. T2 - International Journal of Cosmetic Science T1 - Prebiotic effect of galacto-oligosaccharides on the skin microbiota and determination of their diffusion properties EP - 319 IS - 3 SP - 309 VL - 44 DO - 10.1111/ics.12778 ER -
@article{ author = "Petrov, Anja and Ćorović, Marija and Milivojević, Ana and Simović, Milica and Banjanac, Katarina and Pjanović, Rada and Bezbradica, Dejan", year = "2022", abstract = "Recently, prebiotics are attracting plenty of attention in the field of skin care, since it is found that they are able to support the balance of beneficial and harmful microorganisms on the skin, and accordingly prevent several skin conditions associated with microbial imbalance. Topical application of prebiotics, although insufficiently investigated, holds great promise in improving skin health. The purpose of this research was to determine the prebiotic potential of galacto-oligosaccharides (GOS) for skin microbiota and suitability for incorporation in different topical formulations, and finally, provide insights into their diffusion properties. Methods: The prebiotic effect of GOS was evaluated through the influence on the growth of Staphylococcus epidermidis and Staphylococcus aureus, the most common resident and pathogenic bacterium of the skin microbiota, respectively. Also, with the future use of GOS in cosmetic products in mind, the diffusion of GOS molecules from two different topical formulations, hydrogel and oil-in-water (O/W) gel emulsion, was monitored employing Franz diffusion cell and two systems—with cellulose acetate membrane and transdermal diffusion test model, Strat-M® membrane. Course of fermentation and the amount of diffused GOS molecules were monitored using high-performance liquid chromatography (HPLC). Results: The in vitro results revealed that GOS at a concentration of 5% (w/v) has a pronounced stimulatory effect on S. epidermidis, while simultaneously showing an inhibitory effect on S. aureus, both in nutrient broth and cosmetic formulations. GOS trisaccharide and tetrasaccharide diffusion coefficients from O/W gel emulsion were calculated to be 5.61·10−6 cm2 s−1 and 1.41·10−8 cm2 s−1, respectively. The diffusion coefficient of GOS trisaccharides from hydrogel was 3.22·10−6 cm2 s−1, while it was not determined for tetrasaccharides due to low diffused concentration. Transdermal diffusion tests revealed that GOS incorporated in two formulations stays at the surface of the skin even after 24 h. Conclusion: When applied in adequate concentration, GOS has the potential to be used as a skin prebiotic. Novel GOS enriched formulations, Aristoflex® AVC-based hydrogel and Heliogel™-based O/W gel emulsion, provided efficient diffusion and delivery of prebiotic GOS molecules to the skin surface.", publisher = "John Wiley and Sons Inc.", journal = "International Journal of Cosmetic Science", title = "Prebiotic effect of galacto-oligosaccharides on the skin microbiota and determination of their diffusion properties", pages = "319-309", number = "3", volume = "44", doi = "10.1111/ics.12778" }
Petrov, A., Ćorović, M., Milivojević, A., Simović, M., Banjanac, K., Pjanović, R.,& Bezbradica, D.. (2022). Prebiotic effect of galacto-oligosaccharides on the skin microbiota and determination of their diffusion properties. in International Journal of Cosmetic Science John Wiley and Sons Inc.., 44(3), 309-319. https://doi.org/10.1111/ics.12778
Petrov A, Ćorović M, Milivojević A, Simović M, Banjanac K, Pjanović R, Bezbradica D. Prebiotic effect of galacto-oligosaccharides on the skin microbiota and determination of their diffusion properties. in International Journal of Cosmetic Science. 2022;44(3):309-319. doi:10.1111/ics.12778 .
Petrov, Anja, Ćorović, Marija, Milivojević, Ana, Simović, Milica, Banjanac, Katarina, Pjanović, Rada, Bezbradica, Dejan, "Prebiotic effect of galacto-oligosaccharides on the skin microbiota and determination of their diffusion properties" in International Journal of Cosmetic Science, 44, no. 3 (2022):309-319, https://doi.org/10.1111/ics.12778 . .