Biocompatibility and antibiofilm activity of graphene-oxide functionalized titanium discs and collagen membranes
Само за регистроване кориснике
2022
Аутори
Radunović, MilenaPavić, Aleksandar
Ivanović, Vera
Milivojević, Marija
Radović, Igor
Di Carlo, Roberta
Pilato, Serena
Fontana, Antonella
Piattelli, Adriano
Petrović, Sanja
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The aims of the study were: 1) to evaluate the effect on biofilm formation of barrier membranes and titanium surfaces coated with graphene-oxide (GO); 2) to analyze the connection between the superficial topography of the tested materials and the amount of bacterial accumulation on them and 3) to analyze the biocompatibility of GO functionalized discs using the zebrafish model. Methods: Single species bacterial biofilms (Streptococcus oralis, Veilonella parvula, Fusobacterium nucleatum, Porphyomonas gingivalis) were grown on GO-free membranes, membranes coated with 2 and 10 μg/ml of GO, GO-free and GO-coated titanium discs. The biofilms were analyzed by determining the CFU count and by Scanning Electron Microscopy (SEM) and the materials’ topography by Atomic Force Microscopy (AFM). Zebrafish model was used to determine the materials’ toxicity and inflammatory effects. Results: AFM showed similar roughness of control and GO-coated materials. CFU counts on GO-coated discs were significa...ntly lower than on control discs for all species. CFU counts of S. oralis, V. parvula and P. gingivalis were lower on biofilms grown on both types of GO-coated membranes than on GO-free membrane. SEM analysis showed different formation of single species biofilm of S. oralis on control and GO-coated materials. GO-functionalized titanium discs do not induce toxic or inflammatory effects. Significance: Titanium implant surfaces functionalized with GO have shown to be biocompatible and less susceptible to biofilm formation. These results encourage further in vivo investigation of the tested materials on infection prevention, specifically in prevention and reduction of peri-implant mucositis and periimplantitis incidence.
Кључне речи:
Atomic Force Microscopy / Biocompatible Materials Testing / Biofilm / Dental Material / Graphene oxide / Zebrafish / Peri-Implantitis / Scanning Electron MicroscopyИзвор:
Dental Materials, 2022, 38, 7, 1117-1127Издавач:
- Elsevier Inc.
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200129 (Универзитет у Београду, Стоматолошки факултет) (RS-MESTD-inst-2020-200129)
Институција/група
Inovacioni centarTY - JOUR AU - Radunović, Milena AU - Pavić, Aleksandar AU - Ivanović, Vera AU - Milivojević, Marija AU - Radović, Igor AU - Di Carlo, Roberta AU - Pilato, Serena AU - Fontana, Antonella AU - Piattelli, Adriano AU - Petrović, Sanja PY - 2022 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5144 AB - The aims of the study were: 1) to evaluate the effect on biofilm formation of barrier membranes and titanium surfaces coated with graphene-oxide (GO); 2) to analyze the connection between the superficial topography of the tested materials and the amount of bacterial accumulation on them and 3) to analyze the biocompatibility of GO functionalized discs using the zebrafish model. Methods: Single species bacterial biofilms (Streptococcus oralis, Veilonella parvula, Fusobacterium nucleatum, Porphyomonas gingivalis) were grown on GO-free membranes, membranes coated with 2 and 10 μg/ml of GO, GO-free and GO-coated titanium discs. The biofilms were analyzed by determining the CFU count and by Scanning Electron Microscopy (SEM) and the materials’ topography by Atomic Force Microscopy (AFM). Zebrafish model was used to determine the materials’ toxicity and inflammatory effects. Results: AFM showed similar roughness of control and GO-coated materials. CFU counts on GO-coated discs were significantly lower than on control discs for all species. CFU counts of S. oralis, V. parvula and P. gingivalis were lower on biofilms grown on both types of GO-coated membranes than on GO-free membrane. SEM analysis showed different formation of single species biofilm of S. oralis on control and GO-coated materials. GO-functionalized titanium discs do not induce toxic or inflammatory effects. Significance: Titanium implant surfaces functionalized with GO have shown to be biocompatible and less susceptible to biofilm formation. These results encourage further in vivo investigation of the tested materials on infection prevention, specifically in prevention and reduction of peri-implant mucositis and periimplantitis incidence. PB - Elsevier Inc. T2 - Dental Materials T1 - Biocompatibility and antibiofilm activity of graphene-oxide functionalized titanium discs and collagen membranes EP - 1127 IS - 7 SP - 1117 VL - 38 DO - 10.1016/j.dental.2022.04.024 ER -
@article{ author = "Radunović, Milena and Pavić, Aleksandar and Ivanović, Vera and Milivojević, Marija and Radović, Igor and Di Carlo, Roberta and Pilato, Serena and Fontana, Antonella and Piattelli, Adriano and Petrović, Sanja", year = "2022", abstract = "The aims of the study were: 1) to evaluate the effect on biofilm formation of barrier membranes and titanium surfaces coated with graphene-oxide (GO); 2) to analyze the connection between the superficial topography of the tested materials and the amount of bacterial accumulation on them and 3) to analyze the biocompatibility of GO functionalized discs using the zebrafish model. Methods: Single species bacterial biofilms (Streptococcus oralis, Veilonella parvula, Fusobacterium nucleatum, Porphyomonas gingivalis) were grown on GO-free membranes, membranes coated with 2 and 10 μg/ml of GO, GO-free and GO-coated titanium discs. The biofilms were analyzed by determining the CFU count and by Scanning Electron Microscopy (SEM) and the materials’ topography by Atomic Force Microscopy (AFM). Zebrafish model was used to determine the materials’ toxicity and inflammatory effects. Results: AFM showed similar roughness of control and GO-coated materials. CFU counts on GO-coated discs were significantly lower than on control discs for all species. CFU counts of S. oralis, V. parvula and P. gingivalis were lower on biofilms grown on both types of GO-coated membranes than on GO-free membrane. SEM analysis showed different formation of single species biofilm of S. oralis on control and GO-coated materials. GO-functionalized titanium discs do not induce toxic or inflammatory effects. Significance: Titanium implant surfaces functionalized with GO have shown to be biocompatible and less susceptible to biofilm formation. These results encourage further in vivo investigation of the tested materials on infection prevention, specifically in prevention and reduction of peri-implant mucositis and periimplantitis incidence.", publisher = "Elsevier Inc.", journal = "Dental Materials", title = "Biocompatibility and antibiofilm activity of graphene-oxide functionalized titanium discs and collagen membranes", pages = "1127-1117", number = "7", volume = "38", doi = "10.1016/j.dental.2022.04.024" }
Radunović, M., Pavić, A., Ivanović, V., Milivojević, M., Radović, I., Di Carlo, R., Pilato, S., Fontana, A., Piattelli, A.,& Petrović, S.. (2022). Biocompatibility and antibiofilm activity of graphene-oxide functionalized titanium discs and collagen membranes. in Dental Materials Elsevier Inc.., 38(7), 1117-1127. https://doi.org/10.1016/j.dental.2022.04.024
Radunović M, Pavić A, Ivanović V, Milivojević M, Radović I, Di Carlo R, Pilato S, Fontana A, Piattelli A, Petrović S. Biocompatibility and antibiofilm activity of graphene-oxide functionalized titanium discs and collagen membranes. in Dental Materials. 2022;38(7):1117-1127. doi:10.1016/j.dental.2022.04.024 .
Radunović, Milena, Pavić, Aleksandar, Ivanović, Vera, Milivojević, Marija, Radović, Igor, Di Carlo, Roberta, Pilato, Serena, Fontana, Antonella, Piattelli, Adriano, Petrović, Sanja, "Biocompatibility and antibiofilm activity of graphene-oxide functionalized titanium discs and collagen membranes" in Dental Materials, 38, no. 7 (2022):1117-1127, https://doi.org/10.1016/j.dental.2022.04.024 . .