Polyphenol extraction in microwave reactor using by-product of Thymus serpyllum L. and biological potential of the extract
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2022
Authors
Jovanović, Aleksandra A.Vajić, Una-Jovana V.
Mijin, Dušan Ž.
Zdunić, Gordana M.
Šavikin, Katarina P.
Branković, Suzana
Kitić, Dušanka
Bugarski, Branko M.
Article (Published version)
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In the present study, polyphenol extraction from Thymus serpyllum by-product (herbal dust) in microwave reactor was optimized through varying extraction time, ethanol concentration and solid-to-solvent ratiovia determination of total polyphenol and flavonoid contents, and antioxidant activity, using response surface methodology and a central composite design. Total polyphenol and flavonoids contents, as well as antioxidant activity of the extracts were statistically significantly affected by ethanol concentration and solid-to-solvent ratio. However, extraction time had statistically significant influence only on polyphenol yield. The extraction conditions that maximized polyphenol recovery were 48% ethanol and 0.0402 g/mL solid-to-solvent ratio during 86 s of microwave-assisted extraction, and under these conditions total polyphenol content was estimated to be 57.2 mg of gallic acid equivalents (GAE)/L, while measured value was 58.1 ± 3.2 mg GAE/L. Polyphenol content (HPLC analysis), a...ntioxidant, antimicrobial and antispasmodic potential of the extract prepared under these optimal extraction conditions were evaluated. Rosmarinic acids, luteolin 7-O-glucuronide, caffeic acid, apigenin glucuronide, 6-hydroxyluteolin 7-O-glucoside, 6,8-di-C-glucosylapigenin, and traces of chlorogenic acid were quantified in the extract (descending order of content). The selected extract inhibited 95% ± 0.9 of lipid peroxidation in β-Carotene and linoleic acid emulsion. It was more effective against tested Gram-positive bacteria than against Gram-negative and showed dose-dependent antispasmodic activity on spontaneous contractions, acetylcholine- and potassium chloride-induced contractions. Microwave-assisted extraction could be selected as a successful technique for polyphenol extraction from wild thyme by-product. Biological activities shown in the study open the possibility of potential application of the extract in pharmaceutical, food, functional food and cosmetic products
Keywords:
Antimicrobial activity / Microwave reactor / Polyphenols / Spasmolytic activity / Wild thyme by-productSource:
Journal of Applied Research on Medicinal and Aromatic Plants, 2022, 31, 100417-Publisher:
- Elsevier GmbH
Funding / projects:
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200003 (Institute for Medicinal Plant Research 'Dr. Josif Pančić ', Belgrade) (RS-MESTD-inst-2020-200003)
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200113 (Univeristy of Niš, Faculty of Medicine) (RS-MESTD-inst-2020-200113)
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200135 (University of Belgrade, Faculty of Technology and Metallurgy) (RS-MESTD-inst-2020-200135)
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200287 (Innovation Center of the Faculty of Technology and Metallurgy) (RS-MESTD-inst-2020-200287)
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Inovacioni centarTY - JOUR AU - Jovanović, Aleksandra A. AU - Vajić, Una-Jovana V. AU - Mijin, Dušan Ž. AU - Zdunić, Gordana M. AU - Šavikin, Katarina P. AU - Branković, Suzana AU - Kitić, Dušanka AU - Bugarski, Branko M. PY - 2022 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5194 AB - In the present study, polyphenol extraction from Thymus serpyllum by-product (herbal dust) in microwave reactor was optimized through varying extraction time, ethanol concentration and solid-to-solvent ratiovia determination of total polyphenol and flavonoid contents, and antioxidant activity, using response surface methodology and a central composite design. Total polyphenol and flavonoids contents, as well as antioxidant activity of the extracts were statistically significantly affected by ethanol concentration and solid-to-solvent ratio. However, extraction time had statistically significant influence only on polyphenol yield. The extraction conditions that maximized polyphenol recovery were 48% ethanol and 0.0402 g/mL solid-to-solvent ratio during 86 s of microwave-assisted extraction, and under these conditions total polyphenol content was estimated to be 57.2 mg of gallic acid equivalents (GAE)/L, while measured value was 58.1 ± 3.2 mg GAE/L. Polyphenol content (HPLC analysis), antioxidant, antimicrobial and antispasmodic potential of the extract prepared under these optimal extraction conditions were evaluated. Rosmarinic acids, luteolin 7-O-glucuronide, caffeic acid, apigenin glucuronide, 6-hydroxyluteolin 7-O-glucoside, 6,8-di-C-glucosylapigenin, and traces of chlorogenic acid were quantified in the extract (descending order of content). The selected extract inhibited 95% ± 0.9 of lipid peroxidation in β-Carotene and linoleic acid emulsion. It was more effective against tested Gram-positive bacteria than against Gram-negative and showed dose-dependent antispasmodic activity on spontaneous contractions, acetylcholine- and potassium chloride-induced contractions. Microwave-assisted extraction could be selected as a successful technique for polyphenol extraction from wild thyme by-product. Biological activities shown in the study open the possibility of potential application of the extract in pharmaceutical, food, functional food and cosmetic products PB - Elsevier GmbH T2 - Journal of Applied Research on Medicinal and Aromatic Plants T1 - Polyphenol extraction in microwave reactor using by-product of Thymus serpyllum L. and biological potential of the extract SP - 100417 VL - 31 DO - 10.1016/j.jarmap.2022.100417 ER -
@article{ author = "Jovanović, Aleksandra A. and Vajić, Una-Jovana V. and Mijin, Dušan Ž. and Zdunić, Gordana M. and Šavikin, Katarina P. and Branković, Suzana and Kitić, Dušanka and Bugarski, Branko M.", year = "2022", abstract = "In the present study, polyphenol extraction from Thymus serpyllum by-product (herbal dust) in microwave reactor was optimized through varying extraction time, ethanol concentration and solid-to-solvent ratiovia determination of total polyphenol and flavonoid contents, and antioxidant activity, using response surface methodology and a central composite design. Total polyphenol and flavonoids contents, as well as antioxidant activity of the extracts were statistically significantly affected by ethanol concentration and solid-to-solvent ratio. However, extraction time had statistically significant influence only on polyphenol yield. The extraction conditions that maximized polyphenol recovery were 48% ethanol and 0.0402 g/mL solid-to-solvent ratio during 86 s of microwave-assisted extraction, and under these conditions total polyphenol content was estimated to be 57.2 mg of gallic acid equivalents (GAE)/L, while measured value was 58.1 ± 3.2 mg GAE/L. Polyphenol content (HPLC analysis), antioxidant, antimicrobial and antispasmodic potential of the extract prepared under these optimal extraction conditions were evaluated. Rosmarinic acids, luteolin 7-O-glucuronide, caffeic acid, apigenin glucuronide, 6-hydroxyluteolin 7-O-glucoside, 6,8-di-C-glucosylapigenin, and traces of chlorogenic acid were quantified in the extract (descending order of content). The selected extract inhibited 95% ± 0.9 of lipid peroxidation in β-Carotene and linoleic acid emulsion. It was more effective against tested Gram-positive bacteria than against Gram-negative and showed dose-dependent antispasmodic activity on spontaneous contractions, acetylcholine- and potassium chloride-induced contractions. Microwave-assisted extraction could be selected as a successful technique for polyphenol extraction from wild thyme by-product. Biological activities shown in the study open the possibility of potential application of the extract in pharmaceutical, food, functional food and cosmetic products", publisher = "Elsevier GmbH", journal = "Journal of Applied Research on Medicinal and Aromatic Plants", title = "Polyphenol extraction in microwave reactor using by-product of Thymus serpyllum L. and biological potential of the extract", pages = "100417", volume = "31", doi = "10.1016/j.jarmap.2022.100417" }
Jovanović, A. A., Vajić, U. V., Mijin, D. Ž., Zdunić, G. M., Šavikin, K. P., Branković, S., Kitić, D.,& Bugarski, B. M.. (2022). Polyphenol extraction in microwave reactor using by-product of Thymus serpyllum L. and biological potential of the extract. in Journal of Applied Research on Medicinal and Aromatic Plants Elsevier GmbH., 31, 100417. https://doi.org/10.1016/j.jarmap.2022.100417
Jovanović AA, Vajić UV, Mijin DŽ, Zdunić GM, Šavikin KP, Branković S, Kitić D, Bugarski BM. Polyphenol extraction in microwave reactor using by-product of Thymus serpyllum L. and biological potential of the extract. in Journal of Applied Research on Medicinal and Aromatic Plants. 2022;31:100417. doi:10.1016/j.jarmap.2022.100417 .
Jovanović, Aleksandra A., Vajić, Una-Jovana V., Mijin, Dušan Ž., Zdunić, Gordana M., Šavikin, Katarina P., Branković, Suzana, Kitić, Dušanka, Bugarski, Branko M., "Polyphenol extraction in microwave reactor using by-product of Thymus serpyllum L. and biological potential of the extract" in Journal of Applied Research on Medicinal and Aromatic Plants, 31 (2022):100417, https://doi.org/10.1016/j.jarmap.2022.100417 . .