Ticagrelor Determination via Its Electrooxidation as the Standard, in Tablets and the Spiked Human Serum at Au Solid Electrode
Аутори
Lović, JelenaBogdanović, Aleksandra
Mijin, Dušan
Vuković, Dragan
Petrović, Slobodan
Avramov-Ivić, Milka
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Ticagrelor hydrochloride (TCG) is effective in platelet inhibition and useful for preventing cardiovascular death, myocardial infarction, or ischaemic stroke. The drug standard was investigated by cyclic voltammetry (CV) and quantitatively determined using differential pulse voltammetry (DPV) via its electrooxidation at Au electrode in 0.05 M NaHCO3. DPV showed a linear dependency of the anodic peak currents vs. TCG standard concentrations in the range from 3 x 10-7 mol l-1 to 10-5 mol l-1 with the values of the limit of detection (LOD) and the limit of quantification: 0.288 μmol l-1 and 0.96 μmol l-1, respectively. Using the constructed and validated calibration curve, the values of unknown ticagrelor concentrations in Ticagrex® tablets and in human serum spiked with the standard were determined. Mechanistic study suggests that the oxidation proceeds at sulfur atom by removal of one electron as the rate determining step. In the next, fast step, formed radical cation loose another elec...tron and sulfoxide is formed (with water molecule as nucleophile). The study of TCG degradation showed that at the Au, after 4.5 h of potential cycling, degradation occurs, which was confirmed by UV spectroscopy.
Кључне речи:
ticagrelor hydrochloride / Ticagrex® tablet / human serum / reaction mechanismИзвор:
International Journal of Electrochemical Science, 2022, 17, 220928-Издавач:
- ESG
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200026 (Универзитет у Београду, Институт за хемију, технологију и металургију - ИХТМ) (RS-MESTD-inst-2020-200026)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200135 (Универзитет у Београду, Технолошко-металуршки факултет) (RS-MESTD-inst-2020-200135)
DOI: 10.20964/2022.09.36
ISSN: 1452-3981
WoS: 000884756600028
Scopus: 2-s2.0-85135773451
Институција/група
Tehnološko-metalurški fakultetTY - JOUR AU - Lović, Jelena AU - Bogdanović, Aleksandra AU - Mijin, Dušan AU - Vuković, Dragan AU - Petrović, Slobodan AU - Avramov-Ivić, Milka PY - 2022 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5197 AB - Ticagrelor hydrochloride (TCG) is effective in platelet inhibition and useful for preventing cardiovascular death, myocardial infarction, or ischaemic stroke. The drug standard was investigated by cyclic voltammetry (CV) and quantitatively determined using differential pulse voltammetry (DPV) via its electrooxidation at Au electrode in 0.05 M NaHCO3. DPV showed a linear dependency of the anodic peak currents vs. TCG standard concentrations in the range from 3 x 10-7 mol l-1 to 10-5 mol l-1 with the values of the limit of detection (LOD) and the limit of quantification: 0.288 μmol l-1 and 0.96 μmol l-1, respectively. Using the constructed and validated calibration curve, the values of unknown ticagrelor concentrations in Ticagrex® tablets and in human serum spiked with the standard were determined. Mechanistic study suggests that the oxidation proceeds at sulfur atom by removal of one electron as the rate determining step. In the next, fast step, formed radical cation loose another electron and sulfoxide is formed (with water molecule as nucleophile). The study of TCG degradation showed that at the Au, after 4.5 h of potential cycling, degradation occurs, which was confirmed by UV spectroscopy. PB - ESG T2 - International Journal of Electrochemical Science T1 - Ticagrelor Determination via Its Electrooxidation as the Standard, in Tablets and the Spiked Human Serum at Au Solid Electrode SP - 220928 VL - 17 DO - 10.20964/2022.09.36 ER -
@article{ author = "Lović, Jelena and Bogdanović, Aleksandra and Mijin, Dušan and Vuković, Dragan and Petrović, Slobodan and Avramov-Ivić, Milka", year = "2022", abstract = "Ticagrelor hydrochloride (TCG) is effective in platelet inhibition and useful for preventing cardiovascular death, myocardial infarction, or ischaemic stroke. The drug standard was investigated by cyclic voltammetry (CV) and quantitatively determined using differential pulse voltammetry (DPV) via its electrooxidation at Au electrode in 0.05 M NaHCO3. DPV showed a linear dependency of the anodic peak currents vs. TCG standard concentrations in the range from 3 x 10-7 mol l-1 to 10-5 mol l-1 with the values of the limit of detection (LOD) and the limit of quantification: 0.288 μmol l-1 and 0.96 μmol l-1, respectively. Using the constructed and validated calibration curve, the values of unknown ticagrelor concentrations in Ticagrex® tablets and in human serum spiked with the standard were determined. Mechanistic study suggests that the oxidation proceeds at sulfur atom by removal of one electron as the rate determining step. In the next, fast step, formed radical cation loose another electron and sulfoxide is formed (with water molecule as nucleophile). The study of TCG degradation showed that at the Au, after 4.5 h of potential cycling, degradation occurs, which was confirmed by UV spectroscopy.", publisher = "ESG", journal = "International Journal of Electrochemical Science", title = "Ticagrelor Determination via Its Electrooxidation as the Standard, in Tablets and the Spiked Human Serum at Au Solid Electrode", pages = "220928", volume = "17", doi = "10.20964/2022.09.36" }
Lović, J., Bogdanović, A., Mijin, D., Vuković, D., Petrović, S.,& Avramov-Ivić, M.. (2022). Ticagrelor Determination via Its Electrooxidation as the Standard, in Tablets and the Spiked Human Serum at Au Solid Electrode. in International Journal of Electrochemical Science ESG., 17, 220928. https://doi.org/10.20964/2022.09.36
Lović J, Bogdanović A, Mijin D, Vuković D, Petrović S, Avramov-Ivić M. Ticagrelor Determination via Its Electrooxidation as the Standard, in Tablets and the Spiked Human Serum at Au Solid Electrode. in International Journal of Electrochemical Science. 2022;17:220928. doi:10.20964/2022.09.36 .
Lović, Jelena, Bogdanović, Aleksandra, Mijin, Dušan, Vuković, Dragan, Petrović, Slobodan, Avramov-Ivić, Milka, "Ticagrelor Determination via Its Electrooxidation as the Standard, in Tablets and the Spiked Human Serum at Au Solid Electrode" in International Journal of Electrochemical Science, 17 (2022):220928, https://doi.org/10.20964/2022.09.36 . .