Bioactive Interpenetrating Hydrogel Networks Based on 2-Hydroxyethyl Methacrylate and Gelatin Intertwined with Alginate and Dopped with Apatite as Scaffolding Biomaterials
Authors
Babić Radić, Marija M.Filipović, Vuk V.
Vuković, Jovana S.
Vukomanović, Marija
Rubert, Marina
Hofmann, Sandra
Müller, Ralph
Tomić, Simonida Lj.
Article (Published version)
Metadata
Show full item recordAbstract
Our goal was to create bioimitated scaffolding materials for biomedical purposes. The guiding idea was that we used an interpenetrating structural hierarchy of natural extracellular matrix as a “pattern” to design hydrogel scaffolds that show favorable properties for tissue regeneration. Polymeric hydrogel scaffolds are made in a simple, environmentally friendly way without additional functionalization. Gelatin and 2-hydroxyethyl methacrylate were selected to prepare interpenetrating polymeric networks and linear alginate chains were added as an interpenetrant to study their influence on the scaffold’s functionalities. Cryogelation and porogenation methods were used to obtain the designed scaffolding biomaterials. The scaffold’s structural, morphological, and mechanical properties, in vitro degradation, and cell viability properties were assessed to study the effects of the preparation method and alginate loading. Apatite as an inorganic agent was incorporated into cryogelated scaffold...s to perform an extensive biological assay. Cryogelated scaffolds possess superior functionalities essential for tissue regeneration: fully hydrophilicity, degradability and mechanical features (2.08–9.75 MPa), and an optimal LDH activity. Furthermore, cryogelated scaffolds loaded with apatite showed good cell adhesion capacity, biocompatibility, and non-toxic behavior. All scaffolds performed equally in terms of metabolic activity and osteoconductivity. Cryogelated scaffolds with/without HAp could represent a new advance to promote osteoconductivity and enhance hard tissue repair. The obtained series of scaffolding biomaterials described here can provide a wide range of potential applications in the area of biomedical engineering.
Keywords:
2-hydroxyethyl methacrylate / alginate / biocompatibility / gelatin / hydrogel scaffolding biomaterials / hydroxyapatite / tissue regeneration engineeringSource:
Polymers, 2022, 14, 15, 3112-Publisher:
- MDPI
Funding / projects:
- Chemical and structural designing of nanomaterials for application in medicine and tissue engineering (RS-MESTD-Basic Research (BR or ON)-172026)
- Synthesis and characterization of novel functional polymers and polymeric nanocomposites (RS-MESTD-Basic Research (BR or ON)-172062)
DOI: 10.3390/polym14153112
ISSN: 2073-4360
WoS: 00083907280000
Scopus: 2-s2.0-85137114845
Institution/Community
Tehnološko-metalurški fakultetTY - JOUR AU - Babić Radić, Marija M. AU - Filipović, Vuk V. AU - Vuković, Jovana S. AU - Vukomanović, Marija AU - Rubert, Marina AU - Hofmann, Sandra AU - Müller, Ralph AU - Tomić, Simonida Lj. PY - 2022 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5201 AB - Our goal was to create bioimitated scaffolding materials for biomedical purposes. The guiding idea was that we used an interpenetrating structural hierarchy of natural extracellular matrix as a “pattern” to design hydrogel scaffolds that show favorable properties for tissue regeneration. Polymeric hydrogel scaffolds are made in a simple, environmentally friendly way without additional functionalization. Gelatin and 2-hydroxyethyl methacrylate were selected to prepare interpenetrating polymeric networks and linear alginate chains were added as an interpenetrant to study their influence on the scaffold’s functionalities. Cryogelation and porogenation methods were used to obtain the designed scaffolding biomaterials. The scaffold’s structural, morphological, and mechanical properties, in vitro degradation, and cell viability properties were assessed to study the effects of the preparation method and alginate loading. Apatite as an inorganic agent was incorporated into cryogelated scaffolds to perform an extensive biological assay. Cryogelated scaffolds possess superior functionalities essential for tissue regeneration: fully hydrophilicity, degradability and mechanical features (2.08–9.75 MPa), and an optimal LDH activity. Furthermore, cryogelated scaffolds loaded with apatite showed good cell adhesion capacity, biocompatibility, and non-toxic behavior. All scaffolds performed equally in terms of metabolic activity and osteoconductivity. Cryogelated scaffolds with/without HAp could represent a new advance to promote osteoconductivity and enhance hard tissue repair. The obtained series of scaffolding biomaterials described here can provide a wide range of potential applications in the area of biomedical engineering. PB - MDPI T2 - Polymers T1 - Bioactive Interpenetrating Hydrogel Networks Based on 2-Hydroxyethyl Methacrylate and Gelatin Intertwined with Alginate and Dopped with Apatite as Scaffolding Biomaterials IS - 15 SP - 3112 VL - 14 DO - 10.3390/polym14153112 ER -
@article{ author = "Babić Radić, Marija M. and Filipović, Vuk V. and Vuković, Jovana S. and Vukomanović, Marija and Rubert, Marina and Hofmann, Sandra and Müller, Ralph and Tomić, Simonida Lj.", year = "2022", abstract = "Our goal was to create bioimitated scaffolding materials for biomedical purposes. The guiding idea was that we used an interpenetrating structural hierarchy of natural extracellular matrix as a “pattern” to design hydrogel scaffolds that show favorable properties for tissue regeneration. Polymeric hydrogel scaffolds are made in a simple, environmentally friendly way without additional functionalization. Gelatin and 2-hydroxyethyl methacrylate were selected to prepare interpenetrating polymeric networks and linear alginate chains were added as an interpenetrant to study their influence on the scaffold’s functionalities. Cryogelation and porogenation methods were used to obtain the designed scaffolding biomaterials. The scaffold’s structural, morphological, and mechanical properties, in vitro degradation, and cell viability properties were assessed to study the effects of the preparation method and alginate loading. Apatite as an inorganic agent was incorporated into cryogelated scaffolds to perform an extensive biological assay. Cryogelated scaffolds possess superior functionalities essential for tissue regeneration: fully hydrophilicity, degradability and mechanical features (2.08–9.75 MPa), and an optimal LDH activity. Furthermore, cryogelated scaffolds loaded with apatite showed good cell adhesion capacity, biocompatibility, and non-toxic behavior. All scaffolds performed equally in terms of metabolic activity and osteoconductivity. Cryogelated scaffolds with/without HAp could represent a new advance to promote osteoconductivity and enhance hard tissue repair. The obtained series of scaffolding biomaterials described here can provide a wide range of potential applications in the area of biomedical engineering.", publisher = "MDPI", journal = "Polymers", title = "Bioactive Interpenetrating Hydrogel Networks Based on 2-Hydroxyethyl Methacrylate and Gelatin Intertwined with Alginate and Dopped with Apatite as Scaffolding Biomaterials", number = "15", pages = "3112", volume = "14", doi = "10.3390/polym14153112" }
Babić Radić, M. M., Filipović, V. V., Vuković, J. S., Vukomanović, M., Rubert, M., Hofmann, S., Müller, R.,& Tomić, S. Lj.. (2022). Bioactive Interpenetrating Hydrogel Networks Based on 2-Hydroxyethyl Methacrylate and Gelatin Intertwined with Alginate and Dopped with Apatite as Scaffolding Biomaterials. in Polymers MDPI., 14(15), 3112. https://doi.org/10.3390/polym14153112
Babić Radić MM, Filipović VV, Vuković JS, Vukomanović M, Rubert M, Hofmann S, Müller R, Tomić SL. Bioactive Interpenetrating Hydrogel Networks Based on 2-Hydroxyethyl Methacrylate and Gelatin Intertwined with Alginate and Dopped with Apatite as Scaffolding Biomaterials. in Polymers. 2022;14(15):3112. doi:10.3390/polym14153112 .
Babić Radić, Marija M., Filipović, Vuk V., Vuković, Jovana S., Vukomanović, Marija, Rubert, Marina, Hofmann, Sandra, Müller, Ralph, Tomić, Simonida Lj., "Bioactive Interpenetrating Hydrogel Networks Based on 2-Hydroxyethyl Methacrylate and Gelatin Intertwined with Alginate and Dopped with Apatite as Scaffolding Biomaterials" in Polymers, 14, no. 15 (2022):3112, https://doi.org/10.3390/polym14153112 . .