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dc.creatorStanišić, Marija D.
dc.creatorPopović Kokar, Nikolina
dc.creatorRistić, Predrag
dc.creatorBalaž, Ana Marija
dc.creatorOgnjanović, Miloš
dc.creatorĐokić, Veljko R.
dc.creatorProdanović, Radivoje
dc.creatorTodorović, Tamara R.
dc.date.accessioned2022-12-08T10:00:46Z
dc.date.available2022-12-08T10:00:46Z
dc.date.issued2022
dc.identifier.issn2073-4360
dc.identifier.urihttp://TechnoRep.tmf.bg.ac.rs/handle/123456789/5269
dc.description.abstractMany articles in the literature deal with horseradish peroxidase (HRP) biomineralization, but none pay attention to the isoenzyme composition of commercial HRP or the influence of the carbohydrate component of the protein molecule on the biomineralization process. To study the impact of these factors, we performed periodate oxidation of commercial HRP and a purified HRP-C isoform for biomineralization within ZIF-8. With purified HRP, enzyme@ZIF-8 biocomposites with higher activity were obtained, while periodate oxidation of the carbohydrate component of both commercial HRP and purified HRP-C yields biocomposites with very high activity in acetate buffer that does not degrade the ZIF-8 structure. Using acetate instead of phosphate buffer can prevent the false high activity of HRP@ZIF-8 biocomposites caused by the degradation of ZIF-8 coating. At the same time, purification and especially oxidation of the carbohydrate component of enzymes prior to biomineralization lead to significantly improved activity of the biocomposites.sr
dc.language.isoensr
dc.publisherMDPIsr
dc.relationinfo:eu-repo/grantAgreement/MESTD/inst-2020/200168/RS//sr
dc.relationinfo:eu-repo/grantAgreement/MESTD/inst-2020/200287/RS//sr
dc.relationThis research and APC was funded by the Science Fund of the Republic of Serbia, PROMIS, #6066997, SYMBIOSIS.sr
dc.relation.isreferencedbyhttps://technorep.tmf.bg.ac.rs/handle/123456789/5951
dc.rightsopenAccesssr
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.sourcePolymerssr
dc.subjectbiocatalystssr
dc.subjectbiocompositessr
dc.subjectbiomimetic mineralizationsr
dc.subjecthorseradish peroxidasesr
dc.subjectisoenzymessr
dc.subjectphosphate-acetate buffer influencesr
dc.subjectZIF-8sr
dc.titleThe Influence of Isoenzyme Composition and Chemical Modification on Horseradish Peroxidase@ZIF-8 Biocomposite Performancesr
dc.typearticlesr
dc.rights.licenseBYsr
dc.citation.issue22
dc.citation.rankM21~
dc.citation.spage4834
dc.citation.volume14
dc.description.otherSupplementary information: [https://technorep.tmf.bg.ac.rs/handle/123456789/5951]
dc.identifier.doi10.3390/polym14224834
dc.identifier.fulltexthttp://TechnoRep.tmf.bg.ac.rs/bitstream/id/13048/bitstream_13048.pdf
dc.identifier.scopus2-s2.0-85142455495
dc.type.versionpublishedVersionsr


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