Acrylic modified kraft lignin microspheres as novel support for immobilization of laccase from M.Thermophila expressed in A. oryzae (Novozym® 51003) and application in degradation of anthraquinone textile dyes
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2023
Authors
Salih, RababBanjanac, Katarina
Vukoičić, Ana
Gržetić, Jelena
Popović, Ana
Veljković, Milica
Bezbradica, Dejan
Marinković, Aleksandar
Article (Published version)
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Show full item recordAbstract
The novel lignin-based microspheres (LMS-DEGDMA) were produced through a two-step modification procedure consisting of kraft lignin (KL) modification with acrylic acid synthesized from fructose; and suspension and copolymerization of acrylic acid modified KL (KL-A) with diethylene glycol dimethacrylate (DEGDMA). The applied procedure provided LMS-DEGDMA microspheres in the size range of 50-90 μm with specific surface area of 39 m2/g, porosity ϵp 62, pore diameters of 4-20 nm and total pore volume of 0.14 cm3/g. Immobilization of laccase from Myceliophthora thermophilia expressed in Aspergillus oryzae (Novozyme® 51003) on LMS-DEGDMA was explored. By optimization of the immobilization process, it was demonstrated that laccase immobilization on LMS-DEGDMA exhibited the best results (protein immobilization yield of 70 %, activity yield of 27 %, and catalytic activity of 262 IU/g of support) when the immobilization was carried out at pH 5.0 and 100 mg of offered protein per g support during... 1 h. Additionally, the LMS-DEGDMA-laccase preparation showed efficiency (>80 %, 5-7 cycles) in decolorization of anthraquinone dyes (Lanaset® violet B, Lanaset® blue 2 R and C.I. Acid Green 40) and their mixture. In case of Lanaset® violet B, LMS-DEGDMA improved laccase's kinetics characteristics (90 % over 24 h) along with operational stability (> 63 % catalytic activity, 7 cycles).
Keywords:
Kraft lignin microspheres / Immobilization support / Laccase, decolorization of dyeSource:
Journal of Environmental Chemical Engineering, 2023, 11, 1, 109077-Publisher:
- Elsevier Ltd.
Funding / projects:
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200135 (University of Belgrade, Faculty of Technology and Metallurgy) (RS-MESTD-inst-2020-200135)
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200287 (Innovation Center of the Faculty of Technology and Metallurgy) (RS-MESTD-inst-2020-200287)
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200325 (Military Technical Institute - MTI, Belgrade) (RS-MESTD-inst-2020-200325)
- PrIntPrEnzy - Prebiotics for functional food and bioactive cosmetics produced in intensified enzymatic processes (RS-ScienceFundRS-Ideje-7750109)
- The contribution of COST Action LignoCOST (CA17128), supported by COST (European Cooper- ation in Science and Technology), in promoting interaction, exchange of knowledge and collaborations in the field of lignin valorization is gratefully acknowledged.
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Tehnološko-metalurški fakultetTY - JOUR AU - Salih, Rabab AU - Banjanac, Katarina AU - Vukoičić, Ana AU - Gržetić, Jelena AU - Popović, Ana AU - Veljković, Milica AU - Bezbradica, Dejan AU - Marinković, Aleksandar PY - 2023 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5278 AB - The novel lignin-based microspheres (LMS-DEGDMA) were produced through a two-step modification procedure consisting of kraft lignin (KL) modification with acrylic acid synthesized from fructose; and suspension and copolymerization of acrylic acid modified KL (KL-A) with diethylene glycol dimethacrylate (DEGDMA). The applied procedure provided LMS-DEGDMA microspheres in the size range of 50-90 μm with specific surface area of 39 m2/g, porosity ϵp 62, pore diameters of 4-20 nm and total pore volume of 0.14 cm3/g. Immobilization of laccase from Myceliophthora thermophilia expressed in Aspergillus oryzae (Novozyme® 51003) on LMS-DEGDMA was explored. By optimization of the immobilization process, it was demonstrated that laccase immobilization on LMS-DEGDMA exhibited the best results (protein immobilization yield of 70 %, activity yield of 27 %, and catalytic activity of 262 IU/g of support) when the immobilization was carried out at pH 5.0 and 100 mg of offered protein per g support during 1 h. Additionally, the LMS-DEGDMA-laccase preparation showed efficiency (>80 %, 5-7 cycles) in decolorization of anthraquinone dyes (Lanaset® violet B, Lanaset® blue 2 R and C.I. Acid Green 40) and their mixture. In case of Lanaset® violet B, LMS-DEGDMA improved laccase's kinetics characteristics (90 % over 24 h) along with operational stability (> 63 % catalytic activity, 7 cycles). PB - Elsevier Ltd. T2 - Journal of Environmental Chemical Engineering T1 - Acrylic modified kraft lignin microspheres as novel support for immobilization of laccase from M.Thermophila expressed in A. oryzae (Novozym® 51003) and application in degradation of anthraquinone textile dyes IS - 1 SP - 109077 VL - 11 DO - 10.1016/j.jece.2022.109077 ER -
@article{ author = "Salih, Rabab and Banjanac, Katarina and Vukoičić, Ana and Gržetić, Jelena and Popović, Ana and Veljković, Milica and Bezbradica, Dejan and Marinković, Aleksandar", year = "2023", abstract = "The novel lignin-based microspheres (LMS-DEGDMA) were produced through a two-step modification procedure consisting of kraft lignin (KL) modification with acrylic acid synthesized from fructose; and suspension and copolymerization of acrylic acid modified KL (KL-A) with diethylene glycol dimethacrylate (DEGDMA). The applied procedure provided LMS-DEGDMA microspheres in the size range of 50-90 μm with specific surface area of 39 m2/g, porosity ϵp 62, pore diameters of 4-20 nm and total pore volume of 0.14 cm3/g. Immobilization of laccase from Myceliophthora thermophilia expressed in Aspergillus oryzae (Novozyme® 51003) on LMS-DEGDMA was explored. By optimization of the immobilization process, it was demonstrated that laccase immobilization on LMS-DEGDMA exhibited the best results (protein immobilization yield of 70 %, activity yield of 27 %, and catalytic activity of 262 IU/g of support) when the immobilization was carried out at pH 5.0 and 100 mg of offered protein per g support during 1 h. Additionally, the LMS-DEGDMA-laccase preparation showed efficiency (>80 %, 5-7 cycles) in decolorization of anthraquinone dyes (Lanaset® violet B, Lanaset® blue 2 R and C.I. Acid Green 40) and their mixture. In case of Lanaset® violet B, LMS-DEGDMA improved laccase's kinetics characteristics (90 % over 24 h) along with operational stability (> 63 % catalytic activity, 7 cycles).", publisher = "Elsevier Ltd.", journal = "Journal of Environmental Chemical Engineering", title = "Acrylic modified kraft lignin microspheres as novel support for immobilization of laccase from M.Thermophila expressed in A. oryzae (Novozym® 51003) and application in degradation of anthraquinone textile dyes", number = "1", pages = "109077", volume = "11", doi = "10.1016/j.jece.2022.109077" }
Salih, R., Banjanac, K., Vukoičić, A., Gržetić, J., Popović, A., Veljković, M., Bezbradica, D.,& Marinković, A.. (2023). Acrylic modified kraft lignin microspheres as novel support for immobilization of laccase from M.Thermophila expressed in A. oryzae (Novozym® 51003) and application in degradation of anthraquinone textile dyes. in Journal of Environmental Chemical Engineering Elsevier Ltd.., 11(1), 109077. https://doi.org/10.1016/j.jece.2022.109077
Salih R, Banjanac K, Vukoičić A, Gržetić J, Popović A, Veljković M, Bezbradica D, Marinković A. Acrylic modified kraft lignin microspheres as novel support for immobilization of laccase from M.Thermophila expressed in A. oryzae (Novozym® 51003) and application in degradation of anthraquinone textile dyes. in Journal of Environmental Chemical Engineering. 2023;11(1):109077. doi:10.1016/j.jece.2022.109077 .
Salih, Rabab, Banjanac, Katarina, Vukoičić, Ana, Gržetić, Jelena, Popović, Ana, Veljković, Milica, Bezbradica, Dejan, Marinković, Aleksandar, "Acrylic modified kraft lignin microspheres as novel support for immobilization of laccase from M.Thermophila expressed in A. oryzae (Novozym® 51003) and application in degradation of anthraquinone textile dyes" in Journal of Environmental Chemical Engineering, 11, no. 1 (2023):109077, https://doi.org/10.1016/j.jece.2022.109077 . .