Приказ основних података о документу

dc.creatorVeljković, Milica
dc.creatorSimović, Milica
dc.creatorBanjanac, Katarina
dc.creatorĆorović, Marija
dc.creatorMilivojević, Ana
dc.creatorMilivojević, Milan
dc.creatorBezbradica, Dejan
dc.date.accessioned2022-12-22T17:57:59Z
dc.date.available2022-12-22T17:57:59Z
dc.date.issued2022
dc.identifier.issn2058-9883
dc.identifier.urihttp://TechnoRep.tmf.bg.ac.rs/handle/123456789/5281
dc.description.abstractIn this study, a heterofunctional carrier was obtained by modification of a macroporous polystyrene ion exchange resin with primary amino groups (Purolite® A109) with epichlorohydrin, in view of achieving covalent immobilization of fructosyltransferase (FTase) from a complex enzyme mixture, Pectinex® Ultra SP-L, that is responsible for the synthesis of functionally active fructo-oligosaccharides (FOS). A two-step immobilization protocol, comprising the physical adsorption of FTase performed at pH 4 and subsequent buffer exchange to promote the establishment of covalent bonds at pH 9, was proposed for obtaining a highly active immobilized preparation (243 IU g−1 of support). Additionally, this protocol provided development of a preparation with 20 times more prominent expressed activity of FTase compared to the commercial preparation with predominant pectinase activity. The obtained immobilized preparation was further tested in batch and air-lift reactor systems for FOS synthesis, yielding 52.8% and 54.7% of FOS in total carbohydrates, respectively. Finally, the continuous production of FOS in the air-lift reactor was established for 7 days, with an average FOS yield of 52.5%. Accordingly, it is demonstrated that the immobilization process enabled the development of preparations with exceptional potential for industrial implementation.sr
dc.language.isoensr
dc.publisherRoyal Society of Chemistrysr
dc.relationinfo:eu-repo/grantAgreement/MESTD/inst-2020/200287/RS//sr
dc.relationinfo:eu-repo/grantAgreement/MESTD/inst-2020/200135/RS//sr
dc.relationinfo:eu-repo/grantAgreement/ScienceFundRS/Ideje/7750109/RS//sr
dc.rightsrestrictedAccesssr
dc.sourceReaction Chemistry and Engineeringsr
dc.titleHeterofunctional epoxy support development for immobilization of fructosyltransferase from Pectinex® Ultra SP-L: batch and continuous production of fructo-oligosaccharidessr
dc.typearticlesr
dc.rights.licenseARRsr
dc.citation.epage2526
dc.citation.issue12
dc.citation.rankM21~
dc.citation.spage2518
dc.citation.volume7
dc.identifier.doi10.1039/d2re00182a
dc.identifier.scopus2-s2.0-85143631792
dc.identifier.wos00084465910000
dc.type.versionpublishedVersionsr


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Приказ основних података о документу