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Heterofunctional epoxy support development for immobilization of fructosyltransferase from Pectinex® Ultra SP-L: batch and continuous production of fructo-oligosaccharides
dc.creator | Veljković, Milica | |
dc.creator | Simović, Milica | |
dc.creator | Banjanac, Katarina | |
dc.creator | Ćorović, Marija | |
dc.creator | Milivojević, Ana | |
dc.creator | Milivojević, Milan | |
dc.creator | Bezbradica, Dejan | |
dc.date.accessioned | 2022-12-22T17:57:59Z | |
dc.date.available | 2022-12-22T17:57:59Z | |
dc.date.issued | 2022 | |
dc.identifier.issn | 2058-9883 | |
dc.identifier.uri | http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5281 | |
dc.description.abstract | In this study, a heterofunctional carrier was obtained by modification of a macroporous polystyrene ion exchange resin with primary amino groups (Purolite® A109) with epichlorohydrin, in view of achieving covalent immobilization of fructosyltransferase (FTase) from a complex enzyme mixture, Pectinex® Ultra SP-L, that is responsible for the synthesis of functionally active fructo-oligosaccharides (FOS). A two-step immobilization protocol, comprising the physical adsorption of FTase performed at pH 4 and subsequent buffer exchange to promote the establishment of covalent bonds at pH 9, was proposed for obtaining a highly active immobilized preparation (243 IU g−1 of support). Additionally, this protocol provided development of a preparation with 20 times more prominent expressed activity of FTase compared to the commercial preparation with predominant pectinase activity. The obtained immobilized preparation was further tested in batch and air-lift reactor systems for FOS synthesis, yielding 52.8% and 54.7% of FOS in total carbohydrates, respectively. Finally, the continuous production of FOS in the air-lift reactor was established for 7 days, with an average FOS yield of 52.5%. Accordingly, it is demonstrated that the immobilization process enabled the development of preparations with exceptional potential for industrial implementation. | sr |
dc.language.iso | en | sr |
dc.publisher | Royal Society of Chemistry | sr |
dc.relation | info:eu-repo/grantAgreement/MESTD/inst-2020/200287/RS// | sr |
dc.relation | info:eu-repo/grantAgreement/MESTD/inst-2020/200135/RS// | sr |
dc.relation | info:eu-repo/grantAgreement/ScienceFundRS/Ideje/7750109/RS// | sr |
dc.rights | restrictedAccess | sr |
dc.source | Reaction Chemistry and Engineering | sr |
dc.title | Heterofunctional epoxy support development for immobilization of fructosyltransferase from Pectinex® Ultra SP-L: batch and continuous production of fructo-oligosaccharides | sr |
dc.type | article | sr |
dc.rights.license | ARR | sr |
dc.citation.epage | 2526 | |
dc.citation.issue | 12 | |
dc.citation.rank | M21~ | |
dc.citation.spage | 2518 | |
dc.citation.volume | 7 | |
dc.identifier.doi | 10.1039/d2re00182a | |
dc.identifier.scopus | 2-s2.0-85143631792 | |
dc.identifier.wos | 00084465910000 | |
dc.type.version | publishedVersion | sr |