Invertase was immobilized via its carbohydrate moiety. The immobilized enzyme has a specific activity of 5500 IU g(-1), with 45% activity yield on immobilization. In a packed bed reactor, 90% 2.5 M sucrose was converted at a flow rate of 4 bed volumes h(-1). The obtained specific productivity at 40 degreesC of 3 kg l(-1) h(-1) is the best one so far. Long-term stability was 290 days in 2.5 M sucrose at 40 degreesC and at a flow rate of 3 bed volumes h(-1).
TY - JOUR
AU - Prodanović, Radivoje
AU - Jovanović, Slobodan
AU - Vujčić, Zoran
PY - 2001
UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5343
AB - Invertase was immobilized via its carbohydrate moiety. The immobilized enzyme has a specific activity of 5500 IU g(-1), with 45% activity yield on immobilization. In a packed bed reactor, 90% 2.5 M sucrose was converted at a flow rate of 4 bed volumes h(-1). The obtained specific productivity at 40 degreesC of 3 kg l(-1) h(-1) is the best one so far. Long-term stability was 290 days in 2.5 M sucrose at 40 degreesC and at a flow rate of 3 bed volumes h(-1).
PB - Kluwer Academic Publ, Dordrecht
T2 - Biotechnology Letters
T1 - Immobilization of invertase on a new type of macroporous glycidyl methacrylate
EP - 1174
IS - 14
SP - 1171
VL - 23
DO - 10.1023/A:1010560911400
ER -
@article{
author = "Prodanović, Radivoje and Jovanović, Slobodan and Vujčić, Zoran",
year = "2001",
abstract = "Invertase was immobilized via its carbohydrate moiety. The immobilized enzyme has a specific activity of 5500 IU g(-1), with 45% activity yield on immobilization. In a packed bed reactor, 90% 2.5 M sucrose was converted at a flow rate of 4 bed volumes h(-1). The obtained specific productivity at 40 degreesC of 3 kg l(-1) h(-1) is the best one so far. Long-term stability was 290 days in 2.5 M sucrose at 40 degreesC and at a flow rate of 3 bed volumes h(-1).",
publisher = "Kluwer Academic Publ, Dordrecht",
journal = "Biotechnology Letters",
title = "Immobilization of invertase on a new type of macroporous glycidyl methacrylate",
pages = "1174-1171",
number = "14",
volume = "23",
doi = "10.1023/A:1010560911400"
}
Prodanović, R., Jovanović, S.,& Vujčić, Z.. (2001). Immobilization of invertase on a new type of macroporous glycidyl methacrylate. in Biotechnology Letters
Kluwer Academic Publ, Dordrecht., 23(14), 1171-1174.
https://doi.org/10.1023/A:1010560911400
Prodanović R, Jovanović S, Vujčić Z. Immobilization of invertase on a new type of macroporous glycidyl methacrylate. in Biotechnology Letters. 2001;23(14):1171-1174.
doi:10.1023/A:1010560911400 .
Prodanović, Radivoje, Jovanović, Slobodan, Vujčić, Zoran, "Immobilization of invertase on a new type of macroporous glycidyl methacrylate" in Biotechnology Letters, 23, no. 14 (2001):1171-1174,
https://doi.org/10.1023/A:1010560911400 . .
