Optimization of the extraction of antioxidants from stinging nettle leaf using response surface methodology
Autori
Vajić, Una-JovanaŽivković, Jelena
Ivanov, Milan
Jovović, Đurđica
Šavikin, Katarina
Bugarski, Branko
Mihailović-Stanojević, Nevena
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
The aim of this study was to optimize the parameters for the extraction of total flavonoids from stinging nettle leaf. Comparison of the effects of different solvents on total flavonoid content showed that, regardless of extraction time, aqueous methanolic extracts had higher total flavonoid content than did aqueous ethanolic extracts. So, full factorial design and response surface methodology (RSM) were em-ployed to estimate the effects of methanol content (50, 75 and 100 %) and extraction time (30, 60 and 90 min) on the total flavonoid content and antioxidant capacities of the extracts. RSM analysis showed that methanol content in the solvent influenced significantly total flavonoid content and FRAP (ferric-reducing antioxidant power) antioxidant capacity, while extraction time had no significant effect on either of these responses. Extraction parameters for maximal total flavonoid content were estimated to be 69 % aqueous methanol and 67 min, and 65 % aqueous methanol and 83 min for... maximal FRAP antioxidant capacity. DPPH (2,2-diphenyl-1-picrylhydrazyl) antioxidant capacity was not significantly affected by extraction time or methanol percentage in the solvent.
Ključne reči:
Antioxidant capacity / Flavonoids / Phenolic compounds / Response surface / Urtica dioica l.Izvor:
Macedonian Journal of Chemistry and Chemical Engineering, 2022, 41, 1, 119-128Izdavač:
- Macedonian Journal of Chemistry and Chemical Engineering
Finansiranje / projekti:
- Ministarstvo nauke, tehnološkog razvoja i inovacija Republike Srbije, institucionalno finansiranje - 200003 (Institut za proučavanje lekovitog bilja 'Dr Josif Pančić', Beograd) (RS-MESTD-inst-2020-200003)
- Ministarstvo nauke, tehnološkog razvoja i inovacija Republike Srbije, institucionalno finansiranje - 200015 (Univerzitet u Beogradu, Institut za medicinska istraživanja) (RS-MESTD-inst-2020-200015)
DOI: 10.20450/mjcce.2022.2238
ISSN: 1857-5552
WoS: 00080681140000
Scopus: 2-s2.0-85145861847
Institucija/grupa
Tehnološko-metalurški fakultetTY - JOUR AU - Vajić, Una-Jovana AU - Živković, Jelena AU - Ivanov, Milan AU - Jovović, Đurđica AU - Šavikin, Katarina AU - Bugarski, Branko AU - Mihailović-Stanojević, Nevena PY - 2022 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5561 AB - The aim of this study was to optimize the parameters for the extraction of total flavonoids from stinging nettle leaf. Comparison of the effects of different solvents on total flavonoid content showed that, regardless of extraction time, aqueous methanolic extracts had higher total flavonoid content than did aqueous ethanolic extracts. So, full factorial design and response surface methodology (RSM) were em-ployed to estimate the effects of methanol content (50, 75 and 100 %) and extraction time (30, 60 and 90 min) on the total flavonoid content and antioxidant capacities of the extracts. RSM analysis showed that methanol content in the solvent influenced significantly total flavonoid content and FRAP (ferric-reducing antioxidant power) antioxidant capacity, while extraction time had no significant effect on either of these responses. Extraction parameters for maximal total flavonoid content were estimated to be 69 % aqueous methanol and 67 min, and 65 % aqueous methanol and 83 min for maximal FRAP antioxidant capacity. DPPH (2,2-diphenyl-1-picrylhydrazyl) antioxidant capacity was not significantly affected by extraction time or methanol percentage in the solvent. PB - Macedonian Journal of Chemistry and Chemical Engineering T2 - Macedonian Journal of Chemistry and Chemical Engineering T1 - Optimization of the extraction of antioxidants from stinging nettle leaf using response surface methodology EP - 128 IS - 1 SP - 119 VL - 41 DO - 10.20450/mjcce.2022.2238 ER -
@article{ author = "Vajić, Una-Jovana and Živković, Jelena and Ivanov, Milan and Jovović, Đurđica and Šavikin, Katarina and Bugarski, Branko and Mihailović-Stanojević, Nevena", year = "2022", abstract = "The aim of this study was to optimize the parameters for the extraction of total flavonoids from stinging nettle leaf. Comparison of the effects of different solvents on total flavonoid content showed that, regardless of extraction time, aqueous methanolic extracts had higher total flavonoid content than did aqueous ethanolic extracts. So, full factorial design and response surface methodology (RSM) were em-ployed to estimate the effects of methanol content (50, 75 and 100 %) and extraction time (30, 60 and 90 min) on the total flavonoid content and antioxidant capacities of the extracts. RSM analysis showed that methanol content in the solvent influenced significantly total flavonoid content and FRAP (ferric-reducing antioxidant power) antioxidant capacity, while extraction time had no significant effect on either of these responses. Extraction parameters for maximal total flavonoid content were estimated to be 69 % aqueous methanol and 67 min, and 65 % aqueous methanol and 83 min for maximal FRAP antioxidant capacity. DPPH (2,2-diphenyl-1-picrylhydrazyl) antioxidant capacity was not significantly affected by extraction time or methanol percentage in the solvent.", publisher = "Macedonian Journal of Chemistry and Chemical Engineering", journal = "Macedonian Journal of Chemistry and Chemical Engineering", title = "Optimization of the extraction of antioxidants from stinging nettle leaf using response surface methodology", pages = "128-119", number = "1", volume = "41", doi = "10.20450/mjcce.2022.2238" }
Vajić, U., Živković, J., Ivanov, M., Jovović, Đ., Šavikin, K., Bugarski, B.,& Mihailović-Stanojević, N.. (2022). Optimization of the extraction of antioxidants from stinging nettle leaf using response surface methodology. in Macedonian Journal of Chemistry and Chemical Engineering Macedonian Journal of Chemistry and Chemical Engineering., 41(1), 119-128. https://doi.org/10.20450/mjcce.2022.2238
Vajić U, Živković J, Ivanov M, Jovović Đ, Šavikin K, Bugarski B, Mihailović-Stanojević N. Optimization of the extraction of antioxidants from stinging nettle leaf using response surface methodology. in Macedonian Journal of Chemistry and Chemical Engineering. 2022;41(1):119-128. doi:10.20450/mjcce.2022.2238 .
Vajić, Una-Jovana, Živković, Jelena, Ivanov, Milan, Jovović, Đurđica, Šavikin, Katarina, Bugarski, Branko, Mihailović-Stanojević, Nevena, "Optimization of the extraction of antioxidants from stinging nettle leaf using response surface methodology" in Macedonian Journal of Chemistry and Chemical Engineering, 41, no. 1 (2022):119-128, https://doi.org/10.20450/mjcce.2022.2238 . .