First isolation of methicillin-resistant Staphylococcus aureus from pigs' clinical samples in Serbia
Authors
Žutić, MilenkoĆirković, Ivana
Pavlović, Ljiljana
Ašanin, Jelena

Jovanović, Snežana
Žutić, Jadranka
Ašanin, Ružica
Article (Published version)
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Show full item recordAbstract
Methicillin-resistant Staphylococcus aureus is a highly important human pathogen that is also a significant concern in veterinary medicine. Despite the high prevalence of colonization, clinical infections with methicillin-resistant Staphylococcus aureus appear to be rare in pigs. Methicillin-resistant Staphylococcus aureus was isolated from a sow with endometritis and her five piglets with dermatitis originating from a Serbian farm. Identification of the strains was done by automated system and confirmed by polymerase chain reaction for mecA and nuc genes. Detection of Staphylococcal Cassette Chromosome mec type was performed by multiplex polymerase chain reaction. Antimicrobial susceptibility testing on erythromycin, clindamycin, gentamicin, kanamycin, tobramycin, ciprofloxacin, tetracycline, trimethoprim/sulfamethoxazole and vancomycin was done by disc diffusion method. Six isolated strains from the infected sow and her piglets showed resistance only to tetracycline beside resistance... to all beta-lactam antibiotics. In the tested methicillin-resistant Staphylococcus aureus isolates, Staphylococcal Cassette Chromosome mec type V was present. To our knowledge, this finding is the first documented detection of methicillin-resistant Staphylococcus aureus from pigs' clinical samples in Serbia. The results of our study indicate the emergence of methicillin-resistant Staphylococcus aureus in a pig farm in Serbia highlighting the threat of this antibiotic-resistant microorganism as a pathogen causing both animal and human infections.
Keywords:
Dermatitis / Infection / Metritis / MRSA / Piglets / SowSource:
Acta Veterinaria Brno, 2012, 81, 3, 225-227Publisher:
- University of Veterinary and Pharmaceutical Sciences
Funding / projects:
DOI: 10.2754/avb201281030225
ISSN: 0001-7213
WoS: 000313233000002
Scopus: 2-s2.0-84872064169
Institution/Community
Inovacioni centarTY - JOUR AU - Žutić, Milenko AU - Ćirković, Ivana AU - Pavlović, Ljiljana AU - Ašanin, Jelena AU - Jovanović, Snežana AU - Žutić, Jadranka AU - Ašanin, Ružica PY - 2012 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5686 AB - Methicillin-resistant Staphylococcus aureus is a highly important human pathogen that is also a significant concern in veterinary medicine. Despite the high prevalence of colonization, clinical infections with methicillin-resistant Staphylococcus aureus appear to be rare in pigs. Methicillin-resistant Staphylococcus aureus was isolated from a sow with endometritis and her five piglets with dermatitis originating from a Serbian farm. Identification of the strains was done by automated system and confirmed by polymerase chain reaction for mecA and nuc genes. Detection of Staphylococcal Cassette Chromosome mec type was performed by multiplex polymerase chain reaction. Antimicrobial susceptibility testing on erythromycin, clindamycin, gentamicin, kanamycin, tobramycin, ciprofloxacin, tetracycline, trimethoprim/sulfamethoxazole and vancomycin was done by disc diffusion method. Six isolated strains from the infected sow and her piglets showed resistance only to tetracycline beside resistance to all beta-lactam antibiotics. In the tested methicillin-resistant Staphylococcus aureus isolates, Staphylococcal Cassette Chromosome mec type V was present. To our knowledge, this finding is the first documented detection of methicillin-resistant Staphylococcus aureus from pigs' clinical samples in Serbia. The results of our study indicate the emergence of methicillin-resistant Staphylococcus aureus in a pig farm in Serbia highlighting the threat of this antibiotic-resistant microorganism as a pathogen causing both animal and human infections. PB - University of Veterinary and Pharmaceutical Sciences T2 - Acta Veterinaria Brno T1 - First isolation of methicillin-resistant Staphylococcus aureus from pigs' clinical samples in Serbia EP - 227 IS - 3 SP - 225 VL - 81 DO - 10.2754/avb201281030225 ER -
@article{ author = "Žutić, Milenko and Ćirković, Ivana and Pavlović, Ljiljana and Ašanin, Jelena and Jovanović, Snežana and Žutić, Jadranka and Ašanin, Ružica", year = "2012", abstract = "Methicillin-resistant Staphylococcus aureus is a highly important human pathogen that is also a significant concern in veterinary medicine. Despite the high prevalence of colonization, clinical infections with methicillin-resistant Staphylococcus aureus appear to be rare in pigs. Methicillin-resistant Staphylococcus aureus was isolated from a sow with endometritis and her five piglets with dermatitis originating from a Serbian farm. Identification of the strains was done by automated system and confirmed by polymerase chain reaction for mecA and nuc genes. Detection of Staphylococcal Cassette Chromosome mec type was performed by multiplex polymerase chain reaction. Antimicrobial susceptibility testing on erythromycin, clindamycin, gentamicin, kanamycin, tobramycin, ciprofloxacin, tetracycline, trimethoprim/sulfamethoxazole and vancomycin was done by disc diffusion method. Six isolated strains from the infected sow and her piglets showed resistance only to tetracycline beside resistance to all beta-lactam antibiotics. In the tested methicillin-resistant Staphylococcus aureus isolates, Staphylococcal Cassette Chromosome mec type V was present. To our knowledge, this finding is the first documented detection of methicillin-resistant Staphylococcus aureus from pigs' clinical samples in Serbia. The results of our study indicate the emergence of methicillin-resistant Staphylococcus aureus in a pig farm in Serbia highlighting the threat of this antibiotic-resistant microorganism as a pathogen causing both animal and human infections.", publisher = "University of Veterinary and Pharmaceutical Sciences", journal = "Acta Veterinaria Brno", title = "First isolation of methicillin-resistant Staphylococcus aureus from pigs' clinical samples in Serbia", pages = "227-225", number = "3", volume = "81", doi = "10.2754/avb201281030225" }
Žutić, M., Ćirković, I., Pavlović, L., Ašanin, J., Jovanović, S., Žutić, J.,& Ašanin, R.. (2012). First isolation of methicillin-resistant Staphylococcus aureus from pigs' clinical samples in Serbia. in Acta Veterinaria Brno University of Veterinary and Pharmaceutical Sciences., 81(3), 225-227. https://doi.org/10.2754/avb201281030225
Žutić M, Ćirković I, Pavlović L, Ašanin J, Jovanović S, Žutić J, Ašanin R. First isolation of methicillin-resistant Staphylococcus aureus from pigs' clinical samples in Serbia. in Acta Veterinaria Brno. 2012;81(3):225-227. doi:10.2754/avb201281030225 .
Žutić, Milenko, Ćirković, Ivana, Pavlović, Ljiljana, Ašanin, Jelena, Jovanović, Snežana, Žutić, Jadranka, Ašanin, Ružica, "First isolation of methicillin-resistant Staphylococcus aureus from pigs' clinical samples in Serbia" in Acta Veterinaria Brno, 81, no. 3 (2012):225-227, https://doi.org/10.2754/avb201281030225 . .