Influence of different pre-treatment methods on isolation of extracts with strong antibacterial activity from lichen Usnea barbata using carbon dioxide as a solvent
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2013
Authors
Ivanović, Jasna
Meyer, Florian
Mišić, Dušan

Ašanin, Jelena

Jaeger, Philip
Žižović, Irena

Eggers, Rudolf
Article (Published version)

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Multiplication and spreading of multiresistant pathogenic bacteria that cause fatal infections in humans and animals have boosted research on implementation of newer technologies for producing natural substances with strong antibacterial activities. In this study, SFE from lichen Usnea barbata was investigated for different pre-treatment methods with respect to extracts yield, usnic acid recovery and antibacterial activity against more than thirty methicillin resistant staphylococci strains. Extraction of U. barbata with subcritical carbon dioxide was studied as well. Extraction yield, usnic acid content and antibacterial activity of the extracts were strongly affected by the pre-treatment method. Intensive pre-treatment methods such as flaking and cutting + grinding caused a considerable comminution of the lichen thallus and enabled high extraction yields. On the other hand, mild pre-treatment of the lichen such as impact + shearing enabled isolation of extracts with lower MIC values.... Experimental results indicated that extraction with liquid carbon dioxide at 30 MPa and 25 degrees C was the most favorable condition at which a satisfactory high extraction yield was obtained (2.08%). Extract isolated with liquid carbon dioxide had a high usnic acid content in the extract (632 g/kg) and a very strong antibacterial activity (average MIC of 19.11 mu g/mL). Antibacterial activity of all the extracts against tested strains was comparable to the pure usnic acid or even stronger. This study indicated a potential use of the supercritical extracts of U. barbata in pharmaceutical formulations for treatment of infections caused by staphylococci.
Keywords:
Supercritical CO2 extraction / Subcritical CO2 extraction / Pre-treatment method / Usnea barbata / Antibacterial activity / Methicilin resistant staphylococciSource:
Journal of Supercritical Fluids, 2013, 76, 1-9Publisher:
- Elsevier, Amsterdam
Funding / projects:
- Forschungsgesellschaft Verfahrenstechnik e.V. (GVT) via AiF [16326N]
- Functional physiologically active plant materials with additional values for application in pharmaceutical and food industry (RS-45017)
- German Academic Exchange Service (DAAD)Deutscher Akademischer Austausch Dienst (DAAD)
DOI: 10.1016/j.supflu.2013.01.005
ISSN: 0896-8446
WoS: 000317378500001
Scopus: 2-s2.0-84873697807
Institution/Community
Tehnološko-metalurški fakultetTY - JOUR AU - Ivanović, Jasna AU - Meyer, Florian AU - Mišić, Dušan AU - Ašanin, Jelena AU - Jaeger, Philip AU - Žižović, Irena AU - Eggers, Rudolf PY - 2013 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5700 AB - Multiplication and spreading of multiresistant pathogenic bacteria that cause fatal infections in humans and animals have boosted research on implementation of newer technologies for producing natural substances with strong antibacterial activities. In this study, SFE from lichen Usnea barbata was investigated for different pre-treatment methods with respect to extracts yield, usnic acid recovery and antibacterial activity against more than thirty methicillin resistant staphylococci strains. Extraction of U. barbata with subcritical carbon dioxide was studied as well. Extraction yield, usnic acid content and antibacterial activity of the extracts were strongly affected by the pre-treatment method. Intensive pre-treatment methods such as flaking and cutting + grinding caused a considerable comminution of the lichen thallus and enabled high extraction yields. On the other hand, mild pre-treatment of the lichen such as impact + shearing enabled isolation of extracts with lower MIC values. Experimental results indicated that extraction with liquid carbon dioxide at 30 MPa and 25 degrees C was the most favorable condition at which a satisfactory high extraction yield was obtained (2.08%). Extract isolated with liquid carbon dioxide had a high usnic acid content in the extract (632 g/kg) and a very strong antibacterial activity (average MIC of 19.11 mu g/mL). Antibacterial activity of all the extracts against tested strains was comparable to the pure usnic acid or even stronger. This study indicated a potential use of the supercritical extracts of U. barbata in pharmaceutical formulations for treatment of infections caused by staphylococci. PB - Elsevier, Amsterdam T2 - Journal of Supercritical Fluids T1 - Influence of different pre-treatment methods on isolation of extracts with strong antibacterial activity from lichen Usnea barbata using carbon dioxide as a solvent EP - 9 SP - 1 VL - 76 DO - 10.1016/j.supflu.2013.01.005 ER -
@article{ author = "Ivanović, Jasna and Meyer, Florian and Mišić, Dušan and Ašanin, Jelena and Jaeger, Philip and Žižović, Irena and Eggers, Rudolf", year = "2013", abstract = "Multiplication and spreading of multiresistant pathogenic bacteria that cause fatal infections in humans and animals have boosted research on implementation of newer technologies for producing natural substances with strong antibacterial activities. In this study, SFE from lichen Usnea barbata was investigated for different pre-treatment methods with respect to extracts yield, usnic acid recovery and antibacterial activity against more than thirty methicillin resistant staphylococci strains. Extraction of U. barbata with subcritical carbon dioxide was studied as well. Extraction yield, usnic acid content and antibacterial activity of the extracts were strongly affected by the pre-treatment method. Intensive pre-treatment methods such as flaking and cutting + grinding caused a considerable comminution of the lichen thallus and enabled high extraction yields. On the other hand, mild pre-treatment of the lichen such as impact + shearing enabled isolation of extracts with lower MIC values. Experimental results indicated that extraction with liquid carbon dioxide at 30 MPa and 25 degrees C was the most favorable condition at which a satisfactory high extraction yield was obtained (2.08%). Extract isolated with liquid carbon dioxide had a high usnic acid content in the extract (632 g/kg) and a very strong antibacterial activity (average MIC of 19.11 mu g/mL). Antibacterial activity of all the extracts against tested strains was comparable to the pure usnic acid or even stronger. This study indicated a potential use of the supercritical extracts of U. barbata in pharmaceutical formulations for treatment of infections caused by staphylococci.", publisher = "Elsevier, Amsterdam", journal = "Journal of Supercritical Fluids", title = "Influence of different pre-treatment methods on isolation of extracts with strong antibacterial activity from lichen Usnea barbata using carbon dioxide as a solvent", pages = "9-1", volume = "76", doi = "10.1016/j.supflu.2013.01.005" }
Ivanović, J., Meyer, F., Mišić, D., Ašanin, J., Jaeger, P., Žižović, I.,& Eggers, R.. (2013). Influence of different pre-treatment methods on isolation of extracts with strong antibacterial activity from lichen Usnea barbata using carbon dioxide as a solvent. in Journal of Supercritical Fluids Elsevier, Amsterdam., 76, 1-9. https://doi.org/10.1016/j.supflu.2013.01.005
Ivanović J, Meyer F, Mišić D, Ašanin J, Jaeger P, Žižović I, Eggers R. Influence of different pre-treatment methods on isolation of extracts with strong antibacterial activity from lichen Usnea barbata using carbon dioxide as a solvent. in Journal of Supercritical Fluids. 2013;76:1-9. doi:10.1016/j.supflu.2013.01.005 .
Ivanović, Jasna, Meyer, Florian, Mišić, Dušan, Ašanin, Jelena, Jaeger, Philip, Žižović, Irena, Eggers, Rudolf, "Influence of different pre-treatment methods on isolation of extracts with strong antibacterial activity from lichen Usnea barbata using carbon dioxide as a solvent" in Journal of Supercritical Fluids, 76 (2013):1-9, https://doi.org/10.1016/j.supflu.2013.01.005 . .