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Adsorption of Candida rugosa lipase onto alumina: effect of surface charge

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2015
792.pdf (326.3Kb)
Authors
Zivković, Lidija T. Izrael
Živković, Ljiljana
Jokić, Bojan M.
Savić, Andrija B.
Karadzic, Ivanka M.
Article (Published version)
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Abstract
The impact of the surface charge of alumina supports on the adsorption of Candida rugosa lipase was investigated in terms of the zeta potentials of the adsorption partners. The lipase adhered onto alumina with similar efficiency under both repulsive and attractive electrostatic conditions, shifting the zeta potential of the support towards that of the enzyme. The behavior was explained by a heterogeneous distribution of the surface charge of the lipase molecule. Special emphasis in this study was placed on the effect of immobilization on the enzyme kinetics and principal reasons for enzyme immobilization: improvement in stability and potential for reuse. The enzyme affinity was not altered by its adsorption onto alumina, while the Vmax value of the lipase decreased. The thermostability of the adsorbed lipase was improved. A significant potential for reuse was found.
Keywords:
alumina / lipase / Adsorption / immobilization / Candida rugosa
Source:
Journal of the Serbian Chemical Society, 2015, 80, 9, 1113-1125
Funding / projects:
  • Synthesis, processing and characterization of nanostructured materials for application in the field of energy, mechanical engineering, environmental protection and biomedicine (RS-45012)
  • Simultaneous Bioremediation and Soilification of Degraded Areas to Preserve Natural Resources of Biologically Active Substances, and Development and Production of Biomaterials and Dietetic Products (RS-43004)

DOI: 10.2298/JSC150222035I

ISSN: 0352-5139

WoS: 000364205600002

Scopus: 2-s2.0-84947609900
[ Google Scholar ]
5
3
URI
http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5752
Collections
  • Radovi istraživača / Researchers’ publications (TMF)
Institution/Community
Tehnološko-metalurški fakultet
TY  - JOUR
AU  - Zivković, Lidija T. Izrael
AU  - Živković, Ljiljana
AU  - Jokić, Bojan M.
AU  - Savić, Andrija B.
AU  - Karadzic, Ivanka M.
PY  - 2015
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5752
AB  - The impact of the surface charge of alumina supports on the adsorption of Candida rugosa lipase was investigated in terms of the zeta potentials of the adsorption partners. The lipase adhered onto alumina with similar efficiency under both repulsive and attractive electrostatic conditions, shifting the zeta potential of the support towards that of the enzyme. The behavior was explained by a heterogeneous distribution of the surface charge of the lipase molecule. Special emphasis in this study was placed on the effect of immobilization on the enzyme kinetics and principal reasons for enzyme immobilization: improvement in stability and potential for reuse. The enzyme affinity was not altered by its adsorption onto alumina, while the Vmax value of the lipase decreased. The thermostability of the adsorbed lipase was improved. A significant potential for reuse was found.
T2  - Journal of the Serbian Chemical Society
T1  - Adsorption of Candida rugosa lipase onto alumina: effect of surface charge
EP  - 1125
IS  - 9
SP  - 1113
VL  - 80
DO  - 10.2298/JSC150222035I
ER  - 
@article{
author = "Zivković, Lidija T. Izrael and Živković, Ljiljana and Jokić, Bojan M. and Savić, Andrija B. and Karadzic, Ivanka M.",
year = "2015",
abstract = "The impact of the surface charge of alumina supports on the adsorption of Candida rugosa lipase was investigated in terms of the zeta potentials of the adsorption partners. The lipase adhered onto alumina with similar efficiency under both repulsive and attractive electrostatic conditions, shifting the zeta potential of the support towards that of the enzyme. The behavior was explained by a heterogeneous distribution of the surface charge of the lipase molecule. Special emphasis in this study was placed on the effect of immobilization on the enzyme kinetics and principal reasons for enzyme immobilization: improvement in stability and potential for reuse. The enzyme affinity was not altered by its adsorption onto alumina, while the Vmax value of the lipase decreased. The thermostability of the adsorbed lipase was improved. A significant potential for reuse was found.",
journal = "Journal of the Serbian Chemical Society",
title = "Adsorption of Candida rugosa lipase onto alumina: effect of surface charge",
pages = "1125-1113",
number = "9",
volume = "80",
doi = "10.2298/JSC150222035I"
}
Zivković, L. T. I., Živković, L., Jokić, B. M., Savić, A. B.,& Karadzic, I. M.. (2015). Adsorption of Candida rugosa lipase onto alumina: effect of surface charge. in Journal of the Serbian Chemical Society, 80(9), 1113-1125.
https://doi.org/10.2298/JSC150222035I
Zivković LTI, Živković L, Jokić BM, Savić AB, Karadzic IM. Adsorption of Candida rugosa lipase onto alumina: effect of surface charge. in Journal of the Serbian Chemical Society. 2015;80(9):1113-1125.
doi:10.2298/JSC150222035I .
Zivković, Lidija T. Izrael, Živković, Ljiljana, Jokić, Bojan M., Savić, Andrija B., Karadzic, Ivanka M., "Adsorption of Candida rugosa lipase onto alumina: effect of surface charge" in Journal of the Serbian Chemical Society, 80, no. 9 (2015):1113-1125,
https://doi.org/10.2298/JSC150222035I . .

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