Adsorption of Candida rugosa lipase onto alumina: effect of surface charge

2015
Authors
Zivković, Lidija T. IzraelŽivković, Ljiljana

Jokić, Bojan M.
Savić, Andrija B.

Karadzic, Ivanka M.
Article (Published version)
Metadata
Show full item recordAbstract
The impact of the surface charge of alumina supports on the adsorption of Candida rugosa lipase was investigated in terms of the zeta potentials of the adsorption partners. The lipase adhered onto alumina with similar efficiency under both repulsive and attractive electrostatic conditions, shifting the zeta potential of the support towards that of the enzyme. The behavior was explained by a heterogeneous distribution of the surface charge of the lipase molecule. Special emphasis in this study was placed on the effect of immobilization on the enzyme kinetics and principal reasons for enzyme immobilization: improvement in stability and potential for reuse. The enzyme affinity was not altered by its adsorption onto alumina, while the Vmax value of the lipase decreased. The thermostability of the adsorbed lipase was improved. A significant potential for reuse was found.
Keywords:
alumina / lipase / Adsorption / immobilization / Candida rugosaSource:
Journal of the Serbian Chemical Society, 2015, 80, 9, 1113-1125Funding / projects:
- Synthesis, processing and characterization of nanostructured materials for application in the field of energy, mechanical engineering, environmental protection and biomedicine (RS-45012)
- Simultaneous Bioremediation and Soilification of Degraded Areas to Preserve Natural Resources of Biologically Active Substances, and Development and Production of Biomaterials and Dietetic Products (RS-43004)
DOI: 10.2298/JSC150222035I
ISSN: 0352-5139
WoS: 000364205600002
Scopus: 2-s2.0-84947609900
Institution/Community
Tehnološko-metalurški fakultetTY - JOUR AU - Zivković, Lidija T. Izrael AU - Živković, Ljiljana AU - Jokić, Bojan M. AU - Savić, Andrija B. AU - Karadzic, Ivanka M. PY - 2015 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5752 AB - The impact of the surface charge of alumina supports on the adsorption of Candida rugosa lipase was investigated in terms of the zeta potentials of the adsorption partners. The lipase adhered onto alumina with similar efficiency under both repulsive and attractive electrostatic conditions, shifting the zeta potential of the support towards that of the enzyme. The behavior was explained by a heterogeneous distribution of the surface charge of the lipase molecule. Special emphasis in this study was placed on the effect of immobilization on the enzyme kinetics and principal reasons for enzyme immobilization: improvement in stability and potential for reuse. The enzyme affinity was not altered by its adsorption onto alumina, while the Vmax value of the lipase decreased. The thermostability of the adsorbed lipase was improved. A significant potential for reuse was found. T2 - Journal of the Serbian Chemical Society T1 - Adsorption of Candida rugosa lipase onto alumina: effect of surface charge EP - 1125 IS - 9 SP - 1113 VL - 80 DO - 10.2298/JSC150222035I ER -
@article{ author = "Zivković, Lidija T. Izrael and Živković, Ljiljana and Jokić, Bojan M. and Savić, Andrija B. and Karadzic, Ivanka M.", year = "2015", abstract = "The impact of the surface charge of alumina supports on the adsorption of Candida rugosa lipase was investigated in terms of the zeta potentials of the adsorption partners. The lipase adhered onto alumina with similar efficiency under both repulsive and attractive electrostatic conditions, shifting the zeta potential of the support towards that of the enzyme. The behavior was explained by a heterogeneous distribution of the surface charge of the lipase molecule. Special emphasis in this study was placed on the effect of immobilization on the enzyme kinetics and principal reasons for enzyme immobilization: improvement in stability and potential for reuse. The enzyme affinity was not altered by its adsorption onto alumina, while the Vmax value of the lipase decreased. The thermostability of the adsorbed lipase was improved. A significant potential for reuse was found.", journal = "Journal of the Serbian Chemical Society", title = "Adsorption of Candida rugosa lipase onto alumina: effect of surface charge", pages = "1125-1113", number = "9", volume = "80", doi = "10.2298/JSC150222035I" }
Zivković, L. T. I., Živković, L., Jokić, B. M., Savić, A. B.,& Karadzic, I. M.. (2015). Adsorption of Candida rugosa lipase onto alumina: effect of surface charge. in Journal of the Serbian Chemical Society, 80(9), 1113-1125. https://doi.org/10.2298/JSC150222035I
Zivković LTI, Živković L, Jokić BM, Savić AB, Karadzic IM. Adsorption of Candida rugosa lipase onto alumina: effect of surface charge. in Journal of the Serbian Chemical Society. 2015;80(9):1113-1125. doi:10.2298/JSC150222035I .
Zivković, Lidija T. Izrael, Živković, Ljiljana, Jokić, Bojan M., Savić, Andrija B., Karadzic, Ivanka M., "Adsorption of Candida rugosa lipase onto alumina: effect of surface charge" in Journal of the Serbian Chemical Society, 80, no. 9 (2015):1113-1125, https://doi.org/10.2298/JSC150222035I . .