Boron-containing bioactive glasses (BGs) are being extensively researched for the treatment and regeneration of bone defects because of their osteostimulatory and neovascularization potential. In this study, we report the effects of the ionic dissolution products (IDPs) of different boron-doped, borosilicate, and borate BG scaffolds on mouse bone marrow stromal cells in vitro, using an angiogenesis assay. Five different BG scaffolds of the system SiO2–Na2O–K2O–MgO–CaO–P2O5–B2O3 (with varying amounts of SiO2 and B2O3) were fabricated by the foam replication technique. Bone marrow stromal cells were cultivated in contact with the IDPs of the boron-containing BG scaffolds at different concentrations for 48 h. The expression and secretion of vascular endothelial growth factor (VEGF) from the cultured cells was measured quantitatively using the VEGF ELISA Kit. Cell viability and cell morphology were determined using WST-8 assay and H&E staining, respectively. The cellular response was found... to be dependent on boron content and the B release profile from the glasses corresponded to the positive or negative biological activity of the BGs.
Source:
Journal of Materials Science, 2017, 52, 15, 8785-8792
Publisher:
Springer New York LLC
Funding / projects:
European Commission funding under the 7th Framework Programme (Marie Curie Initial Training Networks; Grant No. 289958, Bioceramics for bone repair).
TY - JOUR
AU - Balasubramanian, P.
AU - Hupa, L.
AU - Jokić, B.
AU - Detsch, R.
AU - Grünewald, A.
AU - Boccaccini, Aldo R.
PY - 2017
UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5820
AB - Boron-containing bioactive glasses (BGs) are being extensively researched for the treatment and regeneration of bone defects because of their osteostimulatory and neovascularization potential. In this study, we report the effects of the ionic dissolution products (IDPs) of different boron-doped, borosilicate, and borate BG scaffolds on mouse bone marrow stromal cells in vitro, using an angiogenesis assay. Five different BG scaffolds of the system SiO2–Na2O–K2O–MgO–CaO–P2O5–B2O3 (with varying amounts of SiO2 and B2O3) were fabricated by the foam replication technique. Bone marrow stromal cells were cultivated in contact with the IDPs of the boron-containing BG scaffolds at different concentrations for 48 h. The expression and secretion of vascular endothelial growth factor (VEGF) from the cultured cells was measured quantitatively using the VEGF ELISA Kit. Cell viability and cell morphology were determined using WST-8 assay and H&E staining, respectively. The cellular response was found to be dependent on boron content and the B release profile from the glasses corresponded to the positive or negative biological activity of the BGs.
PB - Springer New York LLC
T2 - Journal of Materials Science
T1 - Angiogenic potential of boron-containing bioactive glasses: in vitro study
EP - 8792
IS - 15
SP - 8785
VL - 52
DO - 10.1007/s10853-016-0563-7
ER -
@article{
author = "Balasubramanian, P. and Hupa, L. and Jokić, B. and Detsch, R. and Grünewald, A. and Boccaccini, Aldo R.",
year = "2017",
abstract = "Boron-containing bioactive glasses (BGs) are being extensively researched for the treatment and regeneration of bone defects because of their osteostimulatory and neovascularization potential. In this study, we report the effects of the ionic dissolution products (IDPs) of different boron-doped, borosilicate, and borate BG scaffolds on mouse bone marrow stromal cells in vitro, using an angiogenesis assay. Five different BG scaffolds of the system SiO2–Na2O–K2O–MgO–CaO–P2O5–B2O3 (with varying amounts of SiO2 and B2O3) were fabricated by the foam replication technique. Bone marrow stromal cells were cultivated in contact with the IDPs of the boron-containing BG scaffolds at different concentrations for 48 h. The expression and secretion of vascular endothelial growth factor (VEGF) from the cultured cells was measured quantitatively using the VEGF ELISA Kit. Cell viability and cell morphology were determined using WST-8 assay and H&E staining, respectively. The cellular response was found to be dependent on boron content and the B release profile from the glasses corresponded to the positive or negative biological activity of the BGs.",
publisher = "Springer New York LLC",
journal = "Journal of Materials Science",
title = "Angiogenic potential of boron-containing bioactive glasses: in vitro study",
pages = "8792-8785",
number = "15",
volume = "52",
doi = "10.1007/s10853-016-0563-7"
}
Balasubramanian, P., Hupa, L., Jokić, B., Detsch, R., Grünewald, A.,& Boccaccini, A. R.. (2017). Angiogenic potential of boron-containing bioactive glasses: in vitro study. in Journal of Materials Science
Springer New York LLC., 52(15), 8785-8792.
https://doi.org/10.1007/s10853-016-0563-7
Balasubramanian P, Hupa L, Jokić B, Detsch R, Grünewald A, Boccaccini AR. Angiogenic potential of boron-containing bioactive glasses: in vitro study. in Journal of Materials Science. 2017;52(15):8785-8792.
doi:10.1007/s10853-016-0563-7 .
Balasubramanian, P., Hupa, L., Jokić, B., Detsch, R., Grünewald, A., Boccaccini, Aldo R., "Angiogenic potential of boron-containing bioactive glasses: in vitro study" in Journal of Materials Science, 52, no. 15 (2017):8785-8792,
https://doi.org/10.1007/s10853-016-0563-7 . .
