Optimization of solid-phase extraction for analysis of artificial sweeteners in water
Конференцијски прилог (Објављена верзија)
Метаподаци
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After decades of using artificial sweeteners in the food and pharmaceutical industry, their ubiquitous presence in the environment has been determined 1,2. These substances are recognized as emerging pollutants due to limited data on their occurrence, environmental fate and ecotoxicity. Prior to analysis of artificial sweeteners at trace levels in water samples, it is necessary to isolate and concentrate analytes and clean-up the obtained extracts. In this paper, the optimization of the preparation method for the determination of saccharin, sucralose and neotame in water by solid-phase extraction (SPE) was performed. The extracts were analyzed by liquid chromatography–tandem mass spectrometry (LC–MS/MS). In the SPE optimization, the following parameters were tested: the sorbent type, the pH value and the volume of water sample and the elution solvent. The suitability of anion-exchange, polymeric, C18 and carbon sorbents for the extraction of selected sweeteners was assessed. The resu...lts showed that the highest recoveries were obtained using polymeric Oasis HLB cartridges, with copolymer poly(divinylbenzene-co-N-vinylpyrrolidone). Furthermore, it was determined that the pH value of water sample was crucial for extraction efficiency. For all three artificial sweeteners, the optimal pH value of the sample was 3. Finally, the sample volume of 100 ml ensured a high preconcentration factor without the sorbent breakthrough, and methanol was selected as the elution solvent.
Извор:
Šesta konferencija mladih hemičara Srbije, Beograd, Srbija,, 2018, 33-Финансирање / пројекти:
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Институција/група
Inovacioni centarTY - CONF AU - Gvozdić, Eleonora AU - Matić Bujagić, Ivana AU - Tolić Stojadinović, Ljiljana AU - Grujić, Svetlana PY - 2018 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5945 AB - After decades of using artificial sweeteners in the food and pharmaceutical industry, their ubiquitous presence in the environment has been determined 1,2. These substances are recognized as emerging pollutants due to limited data on their occurrence, environmental fate and ecotoxicity. Prior to analysis of artificial sweeteners at trace levels in water samples, it is necessary to isolate and concentrate analytes and clean-up the obtained extracts. In this paper, the optimization of the preparation method for the determination of saccharin, sucralose and neotame in water by solid-phase extraction (SPE) was performed. The extracts were analyzed by liquid chromatography–tandem mass spectrometry (LC–MS/MS). In the SPE optimization, the following parameters were tested: the sorbent type, the pH value and the volume of water sample and the elution solvent. The suitability of anion-exchange, polymeric, C18 and carbon sorbents for the extraction of selected sweeteners was assessed. The results showed that the highest recoveries were obtained using polymeric Oasis HLB cartridges, with copolymer poly(divinylbenzene-co-N-vinylpyrrolidone). Furthermore, it was determined that the pH value of water sample was crucial for extraction efficiency. For all three artificial sweeteners, the optimal pH value of the sample was 3. Finally, the sample volume of 100 ml ensured a high preconcentration factor without the sorbent breakthrough, and methanol was selected as the elution solvent. C3 - Šesta konferencija mladih hemičara Srbije, Beograd, Srbija, T1 - Optimization of solid-phase extraction for analysis of artificial sweeteners in water SP - 33 UR - https://hdl.handle.net/21.15107/rcub_technorep_5945 ER -
@conference{ author = "Gvozdić, Eleonora and Matić Bujagić, Ivana and Tolić Stojadinović, Ljiljana and Grujić, Svetlana", year = "2018", abstract = "After decades of using artificial sweeteners in the food and pharmaceutical industry, their ubiquitous presence in the environment has been determined 1,2. These substances are recognized as emerging pollutants due to limited data on their occurrence, environmental fate and ecotoxicity. Prior to analysis of artificial sweeteners at trace levels in water samples, it is necessary to isolate and concentrate analytes and clean-up the obtained extracts. In this paper, the optimization of the preparation method for the determination of saccharin, sucralose and neotame in water by solid-phase extraction (SPE) was performed. The extracts were analyzed by liquid chromatography–tandem mass spectrometry (LC–MS/MS). In the SPE optimization, the following parameters were tested: the sorbent type, the pH value and the volume of water sample and the elution solvent. The suitability of anion-exchange, polymeric, C18 and carbon sorbents for the extraction of selected sweeteners was assessed. The results showed that the highest recoveries were obtained using polymeric Oasis HLB cartridges, with copolymer poly(divinylbenzene-co-N-vinylpyrrolidone). Furthermore, it was determined that the pH value of water sample was crucial for extraction efficiency. For all three artificial sweeteners, the optimal pH value of the sample was 3. Finally, the sample volume of 100 ml ensured a high preconcentration factor without the sorbent breakthrough, and methanol was selected as the elution solvent.", journal = "Šesta konferencija mladih hemičara Srbije, Beograd, Srbija,", title = "Optimization of solid-phase extraction for analysis of artificial sweeteners in water", pages = "33", url = "https://hdl.handle.net/21.15107/rcub_technorep_5945" }
Gvozdić, E., Matić Bujagić, I., Tolić Stojadinović, L.,& Grujić, S.. (2018). Optimization of solid-phase extraction for analysis of artificial sweeteners in water. in Šesta konferencija mladih hemičara Srbije, Beograd, Srbija,, 33. https://hdl.handle.net/21.15107/rcub_technorep_5945
Gvozdić E, Matić Bujagić I, Tolić Stojadinović L, Grujić S. Optimization of solid-phase extraction for analysis of artificial sweeteners in water. in Šesta konferencija mladih hemičara Srbije, Beograd, Srbija,. 2018;:33. https://hdl.handle.net/21.15107/rcub_technorep_5945 .
Gvozdić, Eleonora, Matić Bujagić, Ivana, Tolić Stojadinović, Ljiljana, Grujić, Svetlana, "Optimization of solid-phase extraction for analysis of artificial sweeteners in water" in Šesta konferencija mladih hemičara Srbije, Beograd, Srbija, (2018):33, https://hdl.handle.net/21.15107/rcub_technorep_5945 .