In Search for Reasons behind Helicobacter pylori Eradication Failure–Assessment of the Antibiotics Resistance Rate and Co-Existence of Helicobacter pylori with Candida Species
Аутори
Bačić, AnaMilivojević, Vladimir
Petković, Isidora
Kekić, Dušan
Gajić, Ina
Medić Brkić, Branislava
Popadić, Dušan
Milosavljević, Tomica
Rajilić-Stojanović, Mirjana
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Helicobacter pylori eradication is characterized by decreasing successful eradication rates. Although treatment failure is primarily associated with resistance to antibiotics, other unknown factors may influence the eradication outcome. This study aimed to assess the presence of the antibiotics resistance genes in H. pylori and the presence of Candida spp., which are proposed to be endosymbiotic hosts of H. pylori, in gastric biopsies of H. pylori-positive patients while simultaneously assessing their relationship. The detection and identification of Candida yeasts and the detection of mutations specific for clarithromycin and fluoroquinolones were performed by using the real-time PCR (RT-PCR) method on DNA extracted from 110 gastric biopsy samples of H. pylori-positive participants. Resistance rate to clarithromycin and fluoroquinolone was 52% and 47%, respectively. Antibiotic resistance was associated with more eradication attempts (p < 0.05). Candida species were detected in nine (8....18%) patients. Candida presence was associated with older age (p < 0.05). A high rate of antibiotic resistance was observed, while Candida presence was scarce, suggesting that endosymbiosis between H. pylori and Candida may not be a major contributing factor to the eradication failure. However, the older age favored Candida gastric mucosa colonization, which could contribute to gastric pathologies and microbiome dysbiosis.
Кључне речи:
Helicobacter pylori / Candida spp. / gastric microbiota / antibiotic resistanceИзвор:
Journal of Fungi, 2023, 9, 3, 328-Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200287 (Иновациони центар Технолошко-металуршког факултета у Београду доо) (RS-MESTD-inst-2020-200287)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200135 (Универзитет у Београду, Технолошко-металуршки факултет) (RS-MESTD-inst-2020-200135)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200110 (Универзитет у Београду, Медицински факултет) (RS-MESTD-inst-2020-200110)
Институција/група
Tehnološko-metalurški fakultetTY - JOUR AU - Bačić, Ana AU - Milivojević, Vladimir AU - Petković, Isidora AU - Kekić, Dušan AU - Gajić, Ina AU - Medić Brkić, Branislava AU - Popadić, Dušan AU - Milosavljević, Tomica AU - Rajilić-Stojanović, Mirjana PY - 2023 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6054 AB - Helicobacter pylori eradication is characterized by decreasing successful eradication rates. Although treatment failure is primarily associated with resistance to antibiotics, other unknown factors may influence the eradication outcome. This study aimed to assess the presence of the antibiotics resistance genes in H. pylori and the presence of Candida spp., which are proposed to be endosymbiotic hosts of H. pylori, in gastric biopsies of H. pylori-positive patients while simultaneously assessing their relationship. The detection and identification of Candida yeasts and the detection of mutations specific for clarithromycin and fluoroquinolones were performed by using the real-time PCR (RT-PCR) method on DNA extracted from 110 gastric biopsy samples of H. pylori-positive participants. Resistance rate to clarithromycin and fluoroquinolone was 52% and 47%, respectively. Antibiotic resistance was associated with more eradication attempts (p < 0.05). Candida species were detected in nine (8.18%) patients. Candida presence was associated with older age (p < 0.05). A high rate of antibiotic resistance was observed, while Candida presence was scarce, suggesting that endosymbiosis between H. pylori and Candida may not be a major contributing factor to the eradication failure. However, the older age favored Candida gastric mucosa colonization, which could contribute to gastric pathologies and microbiome dysbiosis. T2 - Journal of Fungi T1 - In Search for Reasons behind Helicobacter pylori Eradication Failure–Assessment of the Antibiotics Resistance Rate and Co-Existence of Helicobacter pylori with Candida Species IS - 3 SP - 328 VL - 9 DO - 10.3390/jof9030328 ER -
@article{ author = "Bačić, Ana and Milivojević, Vladimir and Petković, Isidora and Kekić, Dušan and Gajić, Ina and Medić Brkić, Branislava and Popadić, Dušan and Milosavljević, Tomica and Rajilić-Stojanović, Mirjana", year = "2023", abstract = "Helicobacter pylori eradication is characterized by decreasing successful eradication rates. Although treatment failure is primarily associated with resistance to antibiotics, other unknown factors may influence the eradication outcome. This study aimed to assess the presence of the antibiotics resistance genes in H. pylori and the presence of Candida spp., which are proposed to be endosymbiotic hosts of H. pylori, in gastric biopsies of H. pylori-positive patients while simultaneously assessing their relationship. The detection and identification of Candida yeasts and the detection of mutations specific for clarithromycin and fluoroquinolones were performed by using the real-time PCR (RT-PCR) method on DNA extracted from 110 gastric biopsy samples of H. pylori-positive participants. Resistance rate to clarithromycin and fluoroquinolone was 52% and 47%, respectively. Antibiotic resistance was associated with more eradication attempts (p < 0.05). Candida species were detected in nine (8.18%) patients. Candida presence was associated with older age (p < 0.05). A high rate of antibiotic resistance was observed, while Candida presence was scarce, suggesting that endosymbiosis between H. pylori and Candida may not be a major contributing factor to the eradication failure. However, the older age favored Candida gastric mucosa colonization, which could contribute to gastric pathologies and microbiome dysbiosis.", journal = "Journal of Fungi", title = "In Search for Reasons behind Helicobacter pylori Eradication Failure–Assessment of the Antibiotics Resistance Rate and Co-Existence of Helicobacter pylori with Candida Species", number = "3", pages = "328", volume = "9", doi = "10.3390/jof9030328" }
Bačić, A., Milivojević, V., Petković, I., Kekić, D., Gajić, I., Medić Brkić, B., Popadić, D., Milosavljević, T.,& Rajilić-Stojanović, M.. (2023). In Search for Reasons behind Helicobacter pylori Eradication Failure–Assessment of the Antibiotics Resistance Rate and Co-Existence of Helicobacter pylori with Candida Species. in Journal of Fungi, 9(3), 328. https://doi.org/10.3390/jof9030328
Bačić A, Milivojević V, Petković I, Kekić D, Gajić I, Medić Brkić B, Popadić D, Milosavljević T, Rajilić-Stojanović M. In Search for Reasons behind Helicobacter pylori Eradication Failure–Assessment of the Antibiotics Resistance Rate and Co-Existence of Helicobacter pylori with Candida Species. in Journal of Fungi. 2023;9(3):328. doi:10.3390/jof9030328 .
Bačić, Ana, Milivojević, Vladimir, Petković, Isidora, Kekić, Dušan, Gajić, Ina, Medić Brkić, Branislava, Popadić, Dušan, Milosavljević, Tomica, Rajilić-Stojanović, Mirjana, "In Search for Reasons behind Helicobacter pylori Eradication Failure–Assessment of the Antibiotics Resistance Rate and Co-Existence of Helicobacter pylori with Candida Species" in Journal of Fungi, 9, no. 3 (2023):328, https://doi.org/10.3390/jof9030328 . .