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The impact of pH value and temperature on polyphenol recovery, antioxidant capacity and physical properties of Serpylli herba's waste extracts

Authorized Users Only
2022
Authors
Jovanović, Aleksandra A.
Petrović, Predrag
Volić, Mina
Obradović, Nataša
Balanč, Bojana
Pravilović, Radoslava
Bugarski, Branko
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Abstract
In the present study, Serpylli herba's waste extracts were prepared using 50% ethanol (pH 6) and 50% ethanol with hydrochloric acid (pH 1.2), using maceration (25 °C) and heat-assisted extraction (HAE, 80 °C). The best extraction conditions were determined via analyzing total polyphenol (TPC) and flavonoid contents (TFC), antioxidant capacity, extraction yield, zeta potential, density, and surface tension. In maceration and HAE, TPC were 1.18±0.06 and 1.43±0.05 mg GAE/mL (pH 6), and 0.69±0.05 and 0.86±0.02 mg GAE/mL (pH 1.2). The same trend is noticed in the case of TFC: 0.33±0.01 and 0.42±0.02 mg CE/mL (pH 6) and 0.24±0.01 and 0.27±0.01 mg CE/mL (pH 1.2). ABTS radical scavenging activity was 775.0±3.5 and 623.8±5.1 mmol Trolox/mL (pH 6), and 269.2±3.1 and 327.9±7.7 mmol Trolox/mL (pH 1.2). In DPPH assay, IC50 was 1.64±0.04 and 1.48±0.03 mg/mL (pH 6), and 7.59±0.07 and 5.11±0.09 mg/mL (pH 1.2). Extraction yield was 0.541±0.049 and 0.583±0.019% (pH 6), and 0.938±0.057 and 0.88...9±0.060% (pH 1.2). Zeta potential was -2.58±0.02 and -2.08±0.10 mV (pH 6), and 1.49±0.37 and 1.08±0.09 mV (pH 1.2). Density was 0.945±0.001 and 0.949±0.001 g/mL (pH 6), and 1.008±0.001 and 1.010±0.001 g/mL (pH 1.2). Surface tension was 29.2±0.3 and 30.5±1.0 mN/m (pH 6), and 32.6±2.3 and 36.5±2.3 mN/m (pH 1.2). The best properties were determined in the extract obtained in HAE at pH 6. The aim of this study is to provide evidence of the physico-chemical properties of Serpylli herba's waste extracts that can add value and improve the quality of existing food, pharmaceutical, and cosmetic products.

Keywords:
antioxidant capacity / density / polyphenols / Serpylli herba waste / surface tension
Source:
XIV CONFERENCE OF CHEMISTS, TECHNOLOGISTS AND ENVIRONMENTALISTS OF REPUBLIC OF SRPSKA BOOK OF PROCEEDINGS, 21-10-2022, 96-104
Publisher:
  • University in Banjaluka, Faculty of Technology

ISBN: 978-99938-54-98-2

[ Google Scholar ]
Handle
https://hdl.handle.net/21.15107/rcub_technorep_6076
URI
http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6076
Collections
  • Radovi istraživača (Inovacioni centar) / Researchers’ publications (Innovation Centre)
Institution/Community
Inovacioni centar
TY  - CONF
AU  - Jovanović, Aleksandra A.
AU  - Petrović, Predrag
AU  - Volić, Mina
AU  - Obradović, Nataša
AU  - Balanč, Bojana
AU  - Pravilović, Radoslava
AU  - Bugarski, Branko
PY  - 2022-10-21
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6076
AB  - In the present study, Serpylli herba's waste extracts were prepared using 50% ethanol (pH 6) and 50%
ethanol with hydrochloric acid (pH 1.2), using maceration (25 °C) and heat-assisted extraction (HAE,
80 °C). The best extraction conditions were determined via analyzing total polyphenol (TPC) and
flavonoid contents (TFC), antioxidant capacity, extraction yield, zeta potential, density, and surface
tension. In maceration and HAE, TPC were 1.18±0.06 and 1.43±0.05 mg GAE/mL (pH 6), and
0.69±0.05 and 0.86±0.02 mg GAE/mL (pH 1.2). The same trend is noticed in the case of TFC:
0.33±0.01 and 0.42±0.02 mg CE/mL (pH 6) and 0.24±0.01 and 0.27±0.01 mg CE/mL (pH 1.2). ABTS
radical scavenging activity was 775.0±3.5 and 623.8±5.1 mmol Trolox/mL (pH 6), and 269.2±3.1
and 327.9±7.7 mmol Trolox/mL (pH 1.2). In DPPH assay, IC50 was 1.64±0.04 and 1.48±0.03 mg/mL
(pH 6), and 7.59±0.07 and 5.11±0.09 mg/mL (pH 1.2). Extraction yield was 0.541±0.049 and
0.583±0.019% (pH 6), and 0.938±0.057 and 0.889±0.060% (pH 1.2). Zeta potential was -2.58±0.02
and -2.08±0.10 mV (pH 6), and 1.49±0.37 and 1.08±0.09 mV (pH 1.2). Density was 0.945±0.001
and 0.949±0.001 g/mL (pH 6), and 1.008±0.001 and 1.010±0.001 g/mL (pH 1.2). Surface tension
was 29.2±0.3 and 30.5±1.0 mN/m (pH 6), and 32.6±2.3 and 36.5±2.3 mN/m (pH 1.2). The best
properties were determined in the extract obtained in HAE at pH 6. The aim of this study is to provide
evidence of the physico-chemical properties of Serpylli herba's waste extracts that can add value and
improve the quality of existing food, pharmaceutical, and cosmetic products.
PB  - University in Banjaluka, Faculty of Technology
C3  - XIV CONFERENCE OF CHEMISTS, TECHNOLOGISTS AND
ENVIRONMENTALISTS OF REPUBLIC OF SRPSKA
BOOK OF PROCEEDINGS
T1  - The impact of pH value and temperature on polyphenol recovery, antioxidant capacity and physical properties of Serpylli herba's waste extracts
EP  - 104
SP  - 96
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6076
ER  - 
@conference{
author = "Jovanović, Aleksandra A. and Petrović, Predrag and Volić, Mina and Obradović, Nataša and Balanč, Bojana and Pravilović, Radoslava and Bugarski, Branko",
year = "2022-10-21",
abstract = "In the present study, Serpylli herba's waste extracts were prepared using 50% ethanol (pH 6) and 50%
ethanol with hydrochloric acid (pH 1.2), using maceration (25 °C) and heat-assisted extraction (HAE,
80 °C). The best extraction conditions were determined via analyzing total polyphenol (TPC) and
flavonoid contents (TFC), antioxidant capacity, extraction yield, zeta potential, density, and surface
tension. In maceration and HAE, TPC were 1.18±0.06 and 1.43±0.05 mg GAE/mL (pH 6), and
0.69±0.05 and 0.86±0.02 mg GAE/mL (pH 1.2). The same trend is noticed in the case of TFC:
0.33±0.01 and 0.42±0.02 mg CE/mL (pH 6) and 0.24±0.01 and 0.27±0.01 mg CE/mL (pH 1.2). ABTS
radical scavenging activity was 775.0±3.5 and 623.8±5.1 mmol Trolox/mL (pH 6), and 269.2±3.1
and 327.9±7.7 mmol Trolox/mL (pH 1.2). In DPPH assay, IC50 was 1.64±0.04 and 1.48±0.03 mg/mL
(pH 6), and 7.59±0.07 and 5.11±0.09 mg/mL (pH 1.2). Extraction yield was 0.541±0.049 and
0.583±0.019% (pH 6), and 0.938±0.057 and 0.889±0.060% (pH 1.2). Zeta potential was -2.58±0.02
and -2.08±0.10 mV (pH 6), and 1.49±0.37 and 1.08±0.09 mV (pH 1.2). Density was 0.945±0.001
and 0.949±0.001 g/mL (pH 6), and 1.008±0.001 and 1.010±0.001 g/mL (pH 1.2). Surface tension
was 29.2±0.3 and 30.5±1.0 mN/m (pH 6), and 32.6±2.3 and 36.5±2.3 mN/m (pH 1.2). The best
properties were determined in the extract obtained in HAE at pH 6. The aim of this study is to provide
evidence of the physico-chemical properties of Serpylli herba's waste extracts that can add value and
improve the quality of existing food, pharmaceutical, and cosmetic products.",
publisher = "University in Banjaluka, Faculty of Technology",
journal = "XIV CONFERENCE OF CHEMISTS, TECHNOLOGISTS AND
ENVIRONMENTALISTS OF REPUBLIC OF SRPSKA
BOOK OF PROCEEDINGS",
title = "The impact of pH value and temperature on polyphenol recovery, antioxidant capacity and physical properties of Serpylli herba's waste extracts",
pages = "104-96",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6076"
}
Jovanović, A. A., Petrović, P., Volić, M., Obradović, N., Balanč, B., Pravilović, R.,& Bugarski, B.. (2022-10-21). The impact of pH value and temperature on polyphenol recovery, antioxidant capacity and physical properties of Serpylli herba's waste extracts. in XIV CONFERENCE OF CHEMISTS, TECHNOLOGISTS AND
ENVIRONMENTALISTS OF REPUBLIC OF SRPSKA
BOOK OF PROCEEDINGS
University in Banjaluka, Faculty of Technology., 96-104.
https://hdl.handle.net/21.15107/rcub_technorep_6076
Jovanović AA, Petrović P, Volić M, Obradović N, Balanč B, Pravilović R, Bugarski B. The impact of pH value and temperature on polyphenol recovery, antioxidant capacity and physical properties of Serpylli herba's waste extracts. in XIV CONFERENCE OF CHEMISTS, TECHNOLOGISTS AND
ENVIRONMENTALISTS OF REPUBLIC OF SRPSKA
BOOK OF PROCEEDINGS. 2022;:96-104.
https://hdl.handle.net/21.15107/rcub_technorep_6076 .
Jovanović, Aleksandra A., Petrović, Predrag, Volić, Mina, Obradović, Nataša, Balanč, Bojana, Pravilović, Radoslava, Bugarski, Branko, "The impact of pH value and temperature on polyphenol recovery, antioxidant capacity and physical properties of Serpylli herba's waste extracts" in XIV CONFERENCE OF CHEMISTS, TECHNOLOGISTS AND
ENVIRONMENTALISTS OF REPUBLIC OF SRPSKA
BOOK OF PROCEEDINGS (2022-10-21):96-104,
https://hdl.handle.net/21.15107/rcub_technorep_6076 .

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