KAOLIN AS A SUPPORT FOR THE IMMOBILIZATION OF HORSERADISH PEROXIDASE: APPLICATION IN ANTHRAQUINONIC DYES DECOLORIZATION FROM WASTEWATER
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2015
Authors
Šekuljica, NatašaPrlainović, Nevena

Mijalković, Jelena

Stefanović, Andrea

Mijin, Dušan

Knežević-Jugović, Zorica

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The world is facing with global water crisis because around 20 % of untreated or partially
treated wastewater is discharged directly into the environment. The main objective of this paper
was to develop an eco-friendly, sustainable biocatalyst for the decolorization of wastewater from
different industries colored with anthraquinonic dyes. Anthraquinonic dyes have fused aromatic
rings, which makes them extremely stabile against photolytic degradation as well as microbial
attack. They are synthesized from antracene, toluene, benzidine so their brakedown products are
toxic, muthagenic, carcinogenic. Natural clay, kaolin was used as a support for the
immobilization of horseradish peroxidase. Prior to immobilization kaolin was activated with
different concentrations of glutaraldehyde (0.1-1%). Next, concentration of the enzyme was
varied in the range 0.5-5 mg cm-3
, and the maximum loading of 2.19 mg g-1 was obtained. The
efficiency of the attained biocatalyst was examined in the... reaction with model dye C. I. Acid
Violet 109. Under defined conditions more than 90 % of the dye was removed within 40 min.
Immobilized HRP approved as a much more stable in a wider pH range than free HRP. Besides,
inhibitory influence of peroxide and dye after immobilization was reduced significantly.
Furthermore, immobilized HRP exhibited reasonable reusability during the decolorization of
tested anthraquinonic dye.
Keywords:
horseradish peroxidase / immobilization / dye / wastewaterSource:
Proceedings / IV međunarodni kongres "Inženjerstvo, ekologija i materijali u procesnoj industriji", Jahorina, 2015, 288-293Publisher:
- Zvornik : Tehnološki fakultet
Funding / projects:
- Novel encapsulation and enzyme technologies for designing of new biocatalysts and biologically active compounds targeting enhancement of food quality, safety and competitiveness (RS-46010)
- Study of the Synthesis, Structure and Activity of Natural and Synthetic Organic Compounds (RS-172013)
Institution/Community
Inovacioni centarTY - CONF AU - Šekuljica, Nataša AU - Prlainović, Nevena AU - Mijalković, Jelena AU - Stefanović, Andrea AU - Mijin, Dušan AU - Knežević-Jugović, Zorica PY - 2015 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6270 AB - The world is facing with global water crisis because around 20 % of untreated or partially treated wastewater is discharged directly into the environment. The main objective of this paper was to develop an eco-friendly, sustainable biocatalyst for the decolorization of wastewater from different industries colored with anthraquinonic dyes. Anthraquinonic dyes have fused aromatic rings, which makes them extremely stabile against photolytic degradation as well as microbial attack. They are synthesized from antracene, toluene, benzidine so their brakedown products are toxic, muthagenic, carcinogenic. Natural clay, kaolin was used as a support for the immobilization of horseradish peroxidase. Prior to immobilization kaolin was activated with different concentrations of glutaraldehyde (0.1-1%). Next, concentration of the enzyme was varied in the range 0.5-5 mg cm-3 , and the maximum loading of 2.19 mg g-1 was obtained. The efficiency of the attained biocatalyst was examined in the reaction with model dye C. I. Acid Violet 109. Under defined conditions more than 90 % of the dye was removed within 40 min. Immobilized HRP approved as a much more stable in a wider pH range than free HRP. Besides, inhibitory influence of peroxide and dye after immobilization was reduced significantly. Furthermore, immobilized HRP exhibited reasonable reusability during the decolorization of tested anthraquinonic dye. PB - Zvornik : Tehnološki fakultet C3 - Proceedings / IV međunarodni kongres "Inženjerstvo, ekologija i materijali u procesnoj industriji", Jahorina T1 - KAOLIN AS A SUPPORT FOR THE IMMOBILIZATION OF HORSERADISH PEROXIDASE: APPLICATION IN ANTHRAQUINONIC DYES DECOLORIZATION FROM WASTEWATER EP - 293 SP - 288 DO - 10.7251/EEMEN1501287S ER -
@conference{ author = "Šekuljica, Nataša and Prlainović, Nevena and Mijalković, Jelena and Stefanović, Andrea and Mijin, Dušan and Knežević-Jugović, Zorica", year = "2015", abstract = "The world is facing with global water crisis because around 20 % of untreated or partially treated wastewater is discharged directly into the environment. The main objective of this paper was to develop an eco-friendly, sustainable biocatalyst for the decolorization of wastewater from different industries colored with anthraquinonic dyes. Anthraquinonic dyes have fused aromatic rings, which makes them extremely stabile against photolytic degradation as well as microbial attack. They are synthesized from antracene, toluene, benzidine so their brakedown products are toxic, muthagenic, carcinogenic. Natural clay, kaolin was used as a support for the immobilization of horseradish peroxidase. Prior to immobilization kaolin was activated with different concentrations of glutaraldehyde (0.1-1%). Next, concentration of the enzyme was varied in the range 0.5-5 mg cm-3 , and the maximum loading of 2.19 mg g-1 was obtained. The efficiency of the attained biocatalyst was examined in the reaction with model dye C. I. Acid Violet 109. Under defined conditions more than 90 % of the dye was removed within 40 min. Immobilized HRP approved as a much more stable in a wider pH range than free HRP. Besides, inhibitory influence of peroxide and dye after immobilization was reduced significantly. Furthermore, immobilized HRP exhibited reasonable reusability during the decolorization of tested anthraquinonic dye.", publisher = "Zvornik : Tehnološki fakultet", journal = "Proceedings / IV međunarodni kongres "Inženjerstvo, ekologija i materijali u procesnoj industriji", Jahorina", title = "KAOLIN AS A SUPPORT FOR THE IMMOBILIZATION OF HORSERADISH PEROXIDASE: APPLICATION IN ANTHRAQUINONIC DYES DECOLORIZATION FROM WASTEWATER", pages = "293-288", doi = "10.7251/EEMEN1501287S" }
Šekuljica, N., Prlainović, N., Mijalković, J., Stefanović, A., Mijin, D.,& Knežević-Jugović, Z.. (2015). KAOLIN AS A SUPPORT FOR THE IMMOBILIZATION OF HORSERADISH PEROXIDASE: APPLICATION IN ANTHRAQUINONIC DYES DECOLORIZATION FROM WASTEWATER. in Proceedings / IV međunarodni kongres "Inženjerstvo, ekologija i materijali u procesnoj industriji", Jahorina Zvornik : Tehnološki fakultet., 288-293. https://doi.org/10.7251/EEMEN1501287S
Šekuljica N, Prlainović N, Mijalković J, Stefanović A, Mijin D, Knežević-Jugović Z. KAOLIN AS A SUPPORT FOR THE IMMOBILIZATION OF HORSERADISH PEROXIDASE: APPLICATION IN ANTHRAQUINONIC DYES DECOLORIZATION FROM WASTEWATER. in Proceedings / IV međunarodni kongres "Inženjerstvo, ekologija i materijali u procesnoj industriji", Jahorina. 2015;:288-293. doi:10.7251/EEMEN1501287S .
Šekuljica, Nataša, Prlainović, Nevena, Mijalković, Jelena, Stefanović, Andrea, Mijin, Dušan, Knežević-Jugović, Zorica, "KAOLIN AS A SUPPORT FOR THE IMMOBILIZATION OF HORSERADISH PEROXIDASE: APPLICATION IN ANTHRAQUINONIC DYES DECOLORIZATION FROM WASTEWATER" in Proceedings / IV međunarodni kongres "Inženjerstvo, ekologija i materijali u procesnoj industriji", Jahorina (2015):288-293, https://doi.org/10.7251/EEMEN1501287S . .