Thymus serpyllum extract loaded liposomes produced by proliposome method
Authors
Jovanović, Aleksandra A.Balanč, Bojana
Petrović, Predrag
Đorđević, Verica
Šavikin, Katarina
Zdunić, Gordana
Bugarski, Branko
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Polyphenols, as secondary metabolites from plants, are used as food complements or ingredients
within the pharmaceutical or cosmetic formulations. However, their use is rather limited, due to
their low bioavailability, integrity, permeability, and solubility. Namely, polyphenols’ sensitivity to
environmental factors during food processing, distribution, or storage, or in the gastrointestinal
tract, also limit their activity and the potential health benefits. Thus, the encapsulation of
polyphenol extracts represents an appropriate way to overcome the mentioned disadvantages.
Thymus serpyllum L. ethanol extract obtained in heat-assisted extraction (80°C), rich in polyphenol
compounds, was encapsulated into liposomes produced using phospholipids and proliposome
method. Total polyphenol content in extract and encapsulation efficiency were determined using the
Folin-Ciocalteu procedure. Particle size, polydispersity index, and zeta potential of empty and
extract loaded liposomes w...ere measured during 28 days using photon correlation spectroscopy.
Total polyphenol content of the extract was amounted to 2.08±0.14 mg of gallic acid
equivalents/mL, whereas encapsulation efficiency was 89.4±0.8%. During the 28-days stability
study, the particle size of empty liposomes varied between 420.6±4.3 and 581.6±3.4 nm with
polydispersity index from 0.109±0.067 to 0.295±0.009, while the size of extract loaded liposomes
was between 278.7±1.5 and 456.4±9.3 nm with polydispersity index 0.179±0.094 to 0.284±0.005.
Zeta potential of empty liposomes varied from -17.1±0.2 to -27.3±0.5 mV, whereas the zeta
potential of extract loaded liposomes was between -13.6±0.3 and -25.4±1.4 mV. The aim of this
study is to provide evidence for food manufacturers and food scientists to make broader use of T.
serpyllum loaded liposomes that can add value and improve the quality of existing food,
pharmaceutical and cosmetic products.
Keywords:
encapsulation efficiency / liposomes / proliposome method / Thymus serpyllumSource:
2nd International UNIfood Conference, 2021, -144Publisher:
- University of Belgrade
Funding / projects:
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200287 (Innovation Center of the Faculty of Technology and Metallurgy) (RS-MESTD-inst-2020-200287)
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200135 (University of Belgrade, Faculty of Technology and Metallurgy) (RS-MESTD-inst-2020-200135)
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200003 (Institute for Medicinal Plant Research 'Dr. Josif Pančić ', Belgrade) (RS-MESTD-inst-2020-200003)
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Institution/Community
Inovacioni centarTY - CONF AU - Jovanović, Aleksandra A. AU - Balanč, Bojana AU - Petrović, Predrag AU - Đorđević, Verica AU - Šavikin, Katarina AU - Zdunić, Gordana AU - Bugarski, Branko PY - 2021 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6410 AB - Polyphenols, as secondary metabolites from plants, are used as food complements or ingredients within the pharmaceutical or cosmetic formulations. However, their use is rather limited, due to their low bioavailability, integrity, permeability, and solubility. Namely, polyphenols’ sensitivity to environmental factors during food processing, distribution, or storage, or in the gastrointestinal tract, also limit their activity and the potential health benefits. Thus, the encapsulation of polyphenol extracts represents an appropriate way to overcome the mentioned disadvantages. Thymus serpyllum L. ethanol extract obtained in heat-assisted extraction (80°C), rich in polyphenol compounds, was encapsulated into liposomes produced using phospholipids and proliposome method. Total polyphenol content in extract and encapsulation efficiency were determined using the Folin-Ciocalteu procedure. Particle size, polydispersity index, and zeta potential of empty and extract loaded liposomes were measured during 28 days using photon correlation spectroscopy. Total polyphenol content of the extract was amounted to 2.08±0.14 mg of gallic acid equivalents/mL, whereas encapsulation efficiency was 89.4±0.8%. During the 28-days stability study, the particle size of empty liposomes varied between 420.6±4.3 and 581.6±3.4 nm with polydispersity index from 0.109±0.067 to 0.295±0.009, while the size of extract loaded liposomes was between 278.7±1.5 and 456.4±9.3 nm with polydispersity index 0.179±0.094 to 0.284±0.005. Zeta potential of empty liposomes varied from -17.1±0.2 to -27.3±0.5 mV, whereas the zeta potential of extract loaded liposomes was between -13.6±0.3 and -25.4±1.4 mV. The aim of this study is to provide evidence for food manufacturers and food scientists to make broader use of T. serpyllum loaded liposomes that can add value and improve the quality of existing food, pharmaceutical and cosmetic products. PB - University of Belgrade C3 - 2nd International UNIfood Conference T1 - Thymus serpyllum extract loaded liposomes produced by proliposome method EP - 144 UR - https://hdl.handle.net/21.15107/rcub_technorep_6410 ER -
@conference{ author = "Jovanović, Aleksandra A. and Balanč, Bojana and Petrović, Predrag and Đorđević, Verica and Šavikin, Katarina and Zdunić, Gordana and Bugarski, Branko", year = "2021", abstract = "Polyphenols, as secondary metabolites from plants, are used as food complements or ingredients within the pharmaceutical or cosmetic formulations. However, their use is rather limited, due to their low bioavailability, integrity, permeability, and solubility. Namely, polyphenols’ sensitivity to environmental factors during food processing, distribution, or storage, or in the gastrointestinal tract, also limit their activity and the potential health benefits. Thus, the encapsulation of polyphenol extracts represents an appropriate way to overcome the mentioned disadvantages. Thymus serpyllum L. ethanol extract obtained in heat-assisted extraction (80°C), rich in polyphenol compounds, was encapsulated into liposomes produced using phospholipids and proliposome method. Total polyphenol content in extract and encapsulation efficiency were determined using the Folin-Ciocalteu procedure. Particle size, polydispersity index, and zeta potential of empty and extract loaded liposomes were measured during 28 days using photon correlation spectroscopy. Total polyphenol content of the extract was amounted to 2.08±0.14 mg of gallic acid equivalents/mL, whereas encapsulation efficiency was 89.4±0.8%. During the 28-days stability study, the particle size of empty liposomes varied between 420.6±4.3 and 581.6±3.4 nm with polydispersity index from 0.109±0.067 to 0.295±0.009, while the size of extract loaded liposomes was between 278.7±1.5 and 456.4±9.3 nm with polydispersity index 0.179±0.094 to 0.284±0.005. Zeta potential of empty liposomes varied from -17.1±0.2 to -27.3±0.5 mV, whereas the zeta potential of extract loaded liposomes was between -13.6±0.3 and -25.4±1.4 mV. The aim of this study is to provide evidence for food manufacturers and food scientists to make broader use of T. serpyllum loaded liposomes that can add value and improve the quality of existing food, pharmaceutical and cosmetic products.", publisher = "University of Belgrade", journal = "2nd International UNIfood Conference", title = "Thymus serpyllum extract loaded liposomes produced by proliposome method", pages = "144", url = "https://hdl.handle.net/21.15107/rcub_technorep_6410" }
Jovanović, A. A., Balanč, B., Petrović, P., Đorđević, V., Šavikin, K., Zdunić, G.,& Bugarski, B.. (2021). Thymus serpyllum extract loaded liposomes produced by proliposome method. in 2nd International UNIfood Conference University of Belgrade.. https://hdl.handle.net/21.15107/rcub_technorep_6410
Jovanović AA, Balanč B, Petrović P, Đorđević V, Šavikin K, Zdunić G, Bugarski B. Thymus serpyllum extract loaded liposomes produced by proliposome method. in 2nd International UNIfood Conference. 2021;:null-144. https://hdl.handle.net/21.15107/rcub_technorep_6410 .
Jovanović, Aleksandra A., Balanč, Bojana, Petrović, Predrag, Đorđević, Verica, Šavikin, Katarina, Zdunić, Gordana, Bugarski, Branko, "Thymus serpyllum extract loaded liposomes produced by proliposome method" in 2nd International UNIfood Conference (2021), https://hdl.handle.net/21.15107/rcub_technorep_6410 .