Karakterizacija liofilizovanih ekstrakata majčine dušice (thymus serpyllum l.) I njihova antioksidativna aktivnost
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2017
Authors
Jovanović, Aleksandra A.Petrović, Predrag
Trifković, Kata
Đorđević, Verica
Lević, Steva
Mijin, Dušan
Bugarski, Branko
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Budući da je većinu aktivnih komponenata biljaka nemoguće sintetisati u
laboratoriji, najbolja opcija je njihova ekstrakcija iz prirodnih izvora. U
datom eksperimentu, korišćena je herba majčine dušice (Thymus serpyllum)
i tri metode ekstrakcije (maceracija, ultrazvučna i mikrotalasna ekstrakcija).
Karakterizacija ekstrakata je urađena kroz određivanje sadržaja polifenola,
flavonoida, šećera i peptida u liofilizovanim ekstraktima majčine dušice.
Metoda ekstrakcije nije imala statistički značajan uticaj na koncentraciju
polifenola u ekstraktima, dok je prinos flavonoida bio statistički značajno
niži u ekstraktu dobijenom u mikrotalasnom reaktoru. Sa druge strane,
procenat šećera i peptida je bio statistički značajno veći u mikrotalasnom
ekstraktu, u poređenju sa ekstraktima dobijenim maceracijom i ultrazvučnim
talasima. Antioksidativna aktivnost ispitana u tri različita testa dala je
sledeće rezultate - sva tri ekstrakta su ostvarila isti antioksidativni potencijal
u neutra...lizaciji ABTS∙+ slobodnog radikala; ekstrakt dobijen ultrazvučnom
sondom pokazao se kao najbolji donor atoma vodonika ili elektrona
slobodnom DPPH.
radikalu; proizvod mikrotalasne ekstrakcije je dostigao
najveći procenat inhibicije oksidativne degradacije β-karotena. Liofilizovani
ekstrakti majčine dušice predstavljaju dobar izvor komponenata značajne
antioksidativne aktivnosti, što im kao aktivnim principima i alternativama
sintetskim antioksidansima, otvara put ka prehrambenoj i farmaceutskoj
industriji, u cilju očuvanja zdravlja ljudi.
Since the majority of plant active substances are impossible to synthesize in
the laboratory, the best option is their extraction from natural sources. In the
present study, wild thyme (Thymus serpyllum) herb and three extraction
techniques were used (maceration, ultrasound- and microwave-assisted
extraction). Characterisation of extracts was carried out through
determination of total polyphenols, flavonoids, sugars and peptides in
lyophilized wild thyme extracts. Extraction technique did not show
statistically significant influence on total polyphenols content, while the
flavonoids yield was significantly lower in extract obtained using microwave
reactor. On the other hand, percentage of sugars and peptides was
statistically higher in microwave extract, in comparison to extracts obtained
using maceration and ultrasound. Antioxidant activity tested in three
different assays has given the following results – all three extracts have
achieved the same antioxidant potential in... neutralization of ABTS∙+ free
radical; extract obtained using ultrasound probe has shown as the best
donor of hydrogen atom or electron to the free DPPH. radical; the product
of microwave-assisted extraction has reached the highest percentage of
preserved β-carotene. Lyophilized wild thyme extracts represent a good
source of components with significant antioxidant activity, which can lead to
their use in food and pharmaceutical industry, as active substances and an
alternative to the synthetic antioxidants, in order to improve human health.
Keywords:
Thymus serpyllum / liofilizacija / sekundarni metaboliti / antioksidativna aktivnost Thymus serpyllum / lyophilization / secondary metabolites / antioxidant activitySource:
V International Congress: „Engineering, Environment and Materials in Processing Industry“, 2017, 1487-1501Publisher:
- Faculty of Technology, Zvornik
Funding / projects:
- Novel encapsulation and enzyme technologies for designing of new biocatalysts and biologically active compounds targeting enhancement of food quality, safety and competitiveness (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46010)
- Traditional and new products of cultivated and wild growing fruits and grape vines, and by-products durring processing, with special emphasis on indigenous varieties: chemical characterization and biological profile (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46013)
Institution/Community
Inovacioni centarTY - CONF AU - Jovanović, Aleksandra A. AU - Petrović, Predrag AU - Trifković, Kata AU - Đorđević, Verica AU - Lević, Steva AU - Mijin, Dušan AU - Bugarski, Branko PY - 2017 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6448 AB - Budući da je većinu aktivnih komponenata biljaka nemoguće sintetisati u laboratoriji, najbolja opcija je njihova ekstrakcija iz prirodnih izvora. U datom eksperimentu, korišćena je herba majčine dušice (Thymus serpyllum) i tri metode ekstrakcije (maceracija, ultrazvučna i mikrotalasna ekstrakcija). Karakterizacija ekstrakata je urađena kroz određivanje sadržaja polifenola, flavonoida, šećera i peptida u liofilizovanim ekstraktima majčine dušice. Metoda ekstrakcije nije imala statistički značajan uticaj na koncentraciju polifenola u ekstraktima, dok je prinos flavonoida bio statistički značajno niži u ekstraktu dobijenom u mikrotalasnom reaktoru. Sa druge strane, procenat šećera i peptida je bio statistički značajno veći u mikrotalasnom ekstraktu, u poređenju sa ekstraktima dobijenim maceracijom i ultrazvučnim talasima. Antioksidativna aktivnost ispitana u tri različita testa dala je sledeće rezultate - sva tri ekstrakta su ostvarila isti antioksidativni potencijal u neutralizaciji ABTS∙+ slobodnog radikala; ekstrakt dobijen ultrazvučnom sondom pokazao se kao najbolji donor atoma vodonika ili elektrona slobodnom DPPH. radikalu; proizvod mikrotalasne ekstrakcije je dostigao najveći procenat inhibicije oksidativne degradacije β-karotena. Liofilizovani ekstrakti majčine dušice predstavljaju dobar izvor komponenata značajne antioksidativne aktivnosti, što im kao aktivnim principima i alternativama sintetskim antioksidansima, otvara put ka prehrambenoj i farmaceutskoj industriji, u cilju očuvanja zdravlja ljudi. AB - Since the majority of plant active substances are impossible to synthesize in the laboratory, the best option is their extraction from natural sources. In the present study, wild thyme (Thymus serpyllum) herb and three extraction techniques were used (maceration, ultrasound- and microwave-assisted extraction). Characterisation of extracts was carried out through determination of total polyphenols, flavonoids, sugars and peptides in lyophilized wild thyme extracts. Extraction technique did not show statistically significant influence on total polyphenols content, while the flavonoids yield was significantly lower in extract obtained using microwave reactor. On the other hand, percentage of sugars and peptides was statistically higher in microwave extract, in comparison to extracts obtained using maceration and ultrasound. Antioxidant activity tested in three different assays has given the following results – all three extracts have achieved the same antioxidant potential in neutralization of ABTS∙+ free radical; extract obtained using ultrasound probe has shown as the best donor of hydrogen atom or electron to the free DPPH. radical; the product of microwave-assisted extraction has reached the highest percentage of preserved β-carotene. Lyophilized wild thyme extracts represent a good source of components with significant antioxidant activity, which can lead to their use in food and pharmaceutical industry, as active substances and an alternative to the synthetic antioxidants, in order to improve human health. PB - Faculty of Technology, Zvornik C3 - V International Congress: „Engineering, Environment and Materials in Processing Industry“ T1 - Karakterizacija liofilizovanih ekstrakata majčine dušice (thymus serpyllum l.) I njihova antioksidativna aktivnost EP - 1501 SP - 1487 DO - 10.7251/EEMSR15011487J ER -
@conference{ author = "Jovanović, Aleksandra A. and Petrović, Predrag and Trifković, Kata and Đorđević, Verica and Lević, Steva and Mijin, Dušan and Bugarski, Branko", year = "2017", abstract = "Budući da je većinu aktivnih komponenata biljaka nemoguće sintetisati u laboratoriji, najbolja opcija je njihova ekstrakcija iz prirodnih izvora. U datom eksperimentu, korišćena je herba majčine dušice (Thymus serpyllum) i tri metode ekstrakcije (maceracija, ultrazvučna i mikrotalasna ekstrakcija). Karakterizacija ekstrakata je urađena kroz određivanje sadržaja polifenola, flavonoida, šećera i peptida u liofilizovanim ekstraktima majčine dušice. Metoda ekstrakcije nije imala statistički značajan uticaj na koncentraciju polifenola u ekstraktima, dok je prinos flavonoida bio statistički značajno niži u ekstraktu dobijenom u mikrotalasnom reaktoru. Sa druge strane, procenat šećera i peptida je bio statistički značajno veći u mikrotalasnom ekstraktu, u poređenju sa ekstraktima dobijenim maceracijom i ultrazvučnim talasima. Antioksidativna aktivnost ispitana u tri različita testa dala je sledeće rezultate - sva tri ekstrakta su ostvarila isti antioksidativni potencijal u neutralizaciji ABTS∙+ slobodnog radikala; ekstrakt dobijen ultrazvučnom sondom pokazao se kao najbolji donor atoma vodonika ili elektrona slobodnom DPPH. radikalu; proizvod mikrotalasne ekstrakcije je dostigao najveći procenat inhibicije oksidativne degradacije β-karotena. Liofilizovani ekstrakti majčine dušice predstavljaju dobar izvor komponenata značajne antioksidativne aktivnosti, što im kao aktivnim principima i alternativama sintetskim antioksidansima, otvara put ka prehrambenoj i farmaceutskoj industriji, u cilju očuvanja zdravlja ljudi., Since the majority of plant active substances are impossible to synthesize in the laboratory, the best option is their extraction from natural sources. In the present study, wild thyme (Thymus serpyllum) herb and three extraction techniques were used (maceration, ultrasound- and microwave-assisted extraction). Characterisation of extracts was carried out through determination of total polyphenols, flavonoids, sugars and peptides in lyophilized wild thyme extracts. Extraction technique did not show statistically significant influence on total polyphenols content, while the flavonoids yield was significantly lower in extract obtained using microwave reactor. On the other hand, percentage of sugars and peptides was statistically higher in microwave extract, in comparison to extracts obtained using maceration and ultrasound. Antioxidant activity tested in three different assays has given the following results – all three extracts have achieved the same antioxidant potential in neutralization of ABTS∙+ free radical; extract obtained using ultrasound probe has shown as the best donor of hydrogen atom or electron to the free DPPH. radical; the product of microwave-assisted extraction has reached the highest percentage of preserved β-carotene. Lyophilized wild thyme extracts represent a good source of components with significant antioxidant activity, which can lead to their use in food and pharmaceutical industry, as active substances and an alternative to the synthetic antioxidants, in order to improve human health.", publisher = "Faculty of Technology, Zvornik", journal = "V International Congress: „Engineering, Environment and Materials in Processing Industry“", title = "Karakterizacija liofilizovanih ekstrakata majčine dušice (thymus serpyllum l.) I njihova antioksidativna aktivnost", pages = "1501-1487", doi = "10.7251/EEMSR15011487J" }
Jovanović, A. A., Petrović, P., Trifković, K., Đorđević, V., Lević, S., Mijin, D.,& Bugarski, B.. (2017). Karakterizacija liofilizovanih ekstrakata majčine dušice (thymus serpyllum l.) I njihova antioksidativna aktivnost. in V International Congress: „Engineering, Environment and Materials in Processing Industry“ Faculty of Technology, Zvornik., 1487-1501. https://doi.org/10.7251/EEMSR15011487J
Jovanović AA, Petrović P, Trifković K, Đorđević V, Lević S, Mijin D, Bugarski B. Karakterizacija liofilizovanih ekstrakata majčine dušice (thymus serpyllum l.) I njihova antioksidativna aktivnost. in V International Congress: „Engineering, Environment and Materials in Processing Industry“. 2017;:1487-1501. doi:10.7251/EEMSR15011487J .
Jovanović, Aleksandra A., Petrović, Predrag, Trifković, Kata, Đorđević, Verica, Lević, Steva, Mijin, Dušan, Bugarski, Branko, "Karakterizacija liofilizovanih ekstrakata majčine dušice (thymus serpyllum l.) I njihova antioksidativna aktivnost" in V International Congress: „Engineering, Environment and Materials in Processing Industry“ (2017):1487-1501, https://doi.org/10.7251/EEMSR15011487J . .