Simulated Body Fluids Prepared with Natural Buffers and System for Active pH Regulation
Samo za registrovane korisnike
2022
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
The current study focuses on creating an initial highly alkaline simulated body fluid whose pH can be decreased to a physiological range by adding CO2 (protein-free simulated body fluid) or a combination of CO2 and human proteins (protein-containing simulated body fluid). The effects of dissolved human proteins, Ca2+ ions, and immersed plasma-sprayed hydroxyapatite coatings on the pH and chemical instability of prepared simulated body fluids were investigated. The physiological concentration of dissolved human proteins decreased the pH instability in prepared simulated body fluids by 60% and the physiological concentration of dissolved Ca2+ ions by 15%. The effect of immersing the hydroxyapatite coatings was negligible. In terms of chemical instability, the dissolution of Ca2+ ions caused the blurring of protein-free simulated body fluids after 0.6-1.0 h. In protein-containing simulated body fluids, this phenomenon was undetectable due to their opacity. The effects of human protein pre...sence on the carbonated-apatite-forming ability on the surfaces of immersed hydroxyapatite coatings in the prepared simulated body fluids were also assessed. The experiments validated the bioactivity of plasma-sprayed hydroxyapatite coatings in the prepared simulated body fluids, regardless of protein presence. On the other hand, under the different experimental conditions (unregulated or regulated pH), the human protein presence had an inhibitory (unregulated pH) or indifferent/promoting (regulated pH) influence on the carbonated-apatite-forming ability. The results of the present study are discussed, as well as the strengths and shortcomings of the prepared simulated body fluids, and are compared to those of previous relevant investigations.
Ključne reči:
Carbonated apatite formation / Human proteins / Hydroxyapatite coatings / pH stability / Simulated body fluidIzvor:
Iranian Journal of Chemistry and Chemical Engineering, 2022, 41, 9, 2918-2935Izdavač:
- Iranian Institute of Research and Development in Chemical Industries
Finansiranje / projekti:
- Ministarstvo nauke, tehnološkog razvoja i inovacija Republike Srbije, institucionalno finansiranje - 200135 (Univerzitet u Beogradu, Tehnološko-metalurški fakultet) (RS-MESTD-inst-2020-200135)
Institucija/grupa
Inovacioni centarTY - JOUR AU - Gligorijević, Bojan R. AU - Vilotijević, Miroljub N. PY - 2022 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6638 AB - The current study focuses on creating an initial highly alkaline simulated body fluid whose pH can be decreased to a physiological range by adding CO2 (protein-free simulated body fluid) or a combination of CO2 and human proteins (protein-containing simulated body fluid). The effects of dissolved human proteins, Ca2+ ions, and immersed plasma-sprayed hydroxyapatite coatings on the pH and chemical instability of prepared simulated body fluids were investigated. The physiological concentration of dissolved human proteins decreased the pH instability in prepared simulated body fluids by 60% and the physiological concentration of dissolved Ca2+ ions by 15%. The effect of immersing the hydroxyapatite coatings was negligible. In terms of chemical instability, the dissolution of Ca2+ ions caused the blurring of protein-free simulated body fluids after 0.6-1.0 h. In protein-containing simulated body fluids, this phenomenon was undetectable due to their opacity. The effects of human protein presence on the carbonated-apatite-forming ability on the surfaces of immersed hydroxyapatite coatings in the prepared simulated body fluids were also assessed. The experiments validated the bioactivity of plasma-sprayed hydroxyapatite coatings in the prepared simulated body fluids, regardless of protein presence. On the other hand, under the different experimental conditions (unregulated or regulated pH), the human protein presence had an inhibitory (unregulated pH) or indifferent/promoting (regulated pH) influence on the carbonated-apatite-forming ability. The results of the present study are discussed, as well as the strengths and shortcomings of the prepared simulated body fluids, and are compared to those of previous relevant investigations. PB - Iranian Institute of Research and Development in Chemical Industries T2 - Iranian Journal of Chemistry and Chemical Engineering T1 - Simulated Body Fluids Prepared with Natural Buffers and System for Active pH Regulation EP - 2935 IS - 9 SP - 2918 VL - 41 DO - 10.30492/ijcce.2021.530070.4823 ER -
@article{ author = "Gligorijević, Bojan R. and Vilotijević, Miroljub N.", year = "2022", abstract = "The current study focuses on creating an initial highly alkaline simulated body fluid whose pH can be decreased to a physiological range by adding CO2 (protein-free simulated body fluid) or a combination of CO2 and human proteins (protein-containing simulated body fluid). The effects of dissolved human proteins, Ca2+ ions, and immersed plasma-sprayed hydroxyapatite coatings on the pH and chemical instability of prepared simulated body fluids were investigated. The physiological concentration of dissolved human proteins decreased the pH instability in prepared simulated body fluids by 60% and the physiological concentration of dissolved Ca2+ ions by 15%. The effect of immersing the hydroxyapatite coatings was negligible. In terms of chemical instability, the dissolution of Ca2+ ions caused the blurring of protein-free simulated body fluids after 0.6-1.0 h. In protein-containing simulated body fluids, this phenomenon was undetectable due to their opacity. The effects of human protein presence on the carbonated-apatite-forming ability on the surfaces of immersed hydroxyapatite coatings in the prepared simulated body fluids were also assessed. The experiments validated the bioactivity of plasma-sprayed hydroxyapatite coatings in the prepared simulated body fluids, regardless of protein presence. On the other hand, under the different experimental conditions (unregulated or regulated pH), the human protein presence had an inhibitory (unregulated pH) or indifferent/promoting (regulated pH) influence on the carbonated-apatite-forming ability. The results of the present study are discussed, as well as the strengths and shortcomings of the prepared simulated body fluids, and are compared to those of previous relevant investigations.", publisher = "Iranian Institute of Research and Development in Chemical Industries", journal = "Iranian Journal of Chemistry and Chemical Engineering", title = "Simulated Body Fluids Prepared with Natural Buffers and System for Active pH Regulation", pages = "2935-2918", number = "9", volume = "41", doi = "10.30492/ijcce.2021.530070.4823" }
Gligorijević, B. R.,& Vilotijević, M. N.. (2022). Simulated Body Fluids Prepared with Natural Buffers and System for Active pH Regulation. in Iranian Journal of Chemistry and Chemical Engineering Iranian Institute of Research and Development in Chemical Industries., 41(9), 2918-2935. https://doi.org/10.30492/ijcce.2021.530070.4823
Gligorijević BR, Vilotijević MN. Simulated Body Fluids Prepared with Natural Buffers and System for Active pH Regulation. in Iranian Journal of Chemistry and Chemical Engineering. 2022;41(9):2918-2935. doi:10.30492/ijcce.2021.530070.4823 .
Gligorijević, Bojan R., Vilotijević, Miroljub N., "Simulated Body Fluids Prepared with Natural Buffers and System for Active pH Regulation" in Iranian Journal of Chemistry and Chemical Engineering, 41, no. 9 (2022):2918-2935, https://doi.org/10.30492/ijcce.2021.530070.4823 . .