Valorization of sunflower meal toward the production of emerging prebiotics
Authorized Users Only
2023
Authors
Veljković, MilicaSimović, Milica
Banjanac, Katarina
Petrov Ivanković, Anja
Nikolić, Valentina
Bezbradica, Dejan
Conference object (Published version)
Metadata
Show full item recordAbstract
Recently, there is an increasing interest in utilizing biomass and waste potential for development of new products and materials. The example of this is sunflower meal, by-product of sunflower seed oil industry, that is currently used as a cheap component of animal feed owing to a poor protein content. The major drawback for their application is high content of complex carbohydrates (15.27% hemicellulose, 12.54% cellulose and 5.88% lignin). Therefore, extraction and enzymatic transformation of carbohydrates (primarily xylan) represent an attractive valorization approach. Since xylan extraction from the plant cell wall is highly restricted by lignin presence, different alkaline extraction methods have been proposed. This work is focused on xylan extraction using ethanol extraction and subsequent delignification approach. The ethanol extraction step yielded fraction rich in simple sugars, polyphenols (predominantly chlorogenic acid) and colorants that proved to have great prebiotic poten...tial for application in skin products enabling proliferation of beneficial skin commensal and suppressing the pathogen one. Introduction of additional delignification step enabled high xylan yields (91.35%). This fraction was subjected to the enzymatic treatment using commercial xylanase (ROHALASE® SEP-VISCO) to obtain xylo-oligosaccharides (XOS), compounds that comprise of 2–10 xylose residues linked via β-1,4-glycosidic bonds with high prebiotic activity and excellent application properties (high temperature and pH stability, good sweetening power and low caloric value) for food industry. As result of the sunflower meal xylan treatment under optimum reaction conditions (60°C, pH6 and enzyme concentration of 0.5% per substrate), XOS with a polymerization degree of 2–6 were produced (with predominance of XOS2), without xylose generation. The prebiotic activity of the obtained XOS fraction was confirmed by fermentation with beneficial microbial cultures and pathogenic gut microbiota representative. Therefore, it was shown that significant portion of meal can be converted to added-value functional ingredients, while remaining product retains higher protein content.
Keywords:
sunflower meal / xylo-oligosaccharides / xylanase / emerging prebiotics / skin prebioticsSource:
Book of Abstracts / International Scientific Conference Green Agenda for Western Balkans, Belgrade, June 20-22, 2023, 2023, 36-Publisher:
- Belgrade : University, Faculty of Geography
Funding / projects:
- PrIntPrEnzy - Prebiotics for functional food and bioactive cosmetics produced in intensified enzymatic processes (RS-ScienceFundRS-Ideje-7750109)
- Horizon Europe 2021-2027 research and innovation programme under grant agreement ID 101060130
Collections
Institution/Community
Inovacioni centarTY - CONF AU - Veljković, Milica AU - Simović, Milica AU - Banjanac, Katarina AU - Petrov Ivanković, Anja AU - Nikolić, Valentina AU - Bezbradica, Dejan PY - 2023 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6842 AB - Recently, there is an increasing interest in utilizing biomass and waste potential for development of new products and materials. The example of this is sunflower meal, by-product of sunflower seed oil industry, that is currently used as a cheap component of animal feed owing to a poor protein content. The major drawback for their application is high content of complex carbohydrates (15.27% hemicellulose, 12.54% cellulose and 5.88% lignin). Therefore, extraction and enzymatic transformation of carbohydrates (primarily xylan) represent an attractive valorization approach. Since xylan extraction from the plant cell wall is highly restricted by lignin presence, different alkaline extraction methods have been proposed. This work is focused on xylan extraction using ethanol extraction and subsequent delignification approach. The ethanol extraction step yielded fraction rich in simple sugars, polyphenols (predominantly chlorogenic acid) and colorants that proved to have great prebiotic potential for application in skin products enabling proliferation of beneficial skin commensal and suppressing the pathogen one. Introduction of additional delignification step enabled high xylan yields (91.35%). This fraction was subjected to the enzymatic treatment using commercial xylanase (ROHALASE® SEP-VISCO) to obtain xylo-oligosaccharides (XOS), compounds that comprise of 2–10 xylose residues linked via β-1,4-glycosidic bonds with high prebiotic activity and excellent application properties (high temperature and pH stability, good sweetening power and low caloric value) for food industry. As result of the sunflower meal xylan treatment under optimum reaction conditions (60°C, pH6 and enzyme concentration of 0.5% per substrate), XOS with a polymerization degree of 2–6 were produced (with predominance of XOS2), without xylose generation. The prebiotic activity of the obtained XOS fraction was confirmed by fermentation with beneficial microbial cultures and pathogenic gut microbiota representative. Therefore, it was shown that significant portion of meal can be converted to added-value functional ingredients, while remaining product retains higher protein content. PB - Belgrade : University, Faculty of Geography C3 - Book of Abstracts / International Scientific Conference Green Agenda for Western Balkans, Belgrade, June 20-22, 2023 T1 - Valorization of sunflower meal toward the production of emerging prebiotics SP - 36 UR - https://hdl.handle.net/21.15107/rcub_technorep_6842 ER -
@conference{ author = "Veljković, Milica and Simović, Milica and Banjanac, Katarina and Petrov Ivanković, Anja and Nikolić, Valentina and Bezbradica, Dejan", year = "2023", abstract = "Recently, there is an increasing interest in utilizing biomass and waste potential for development of new products and materials. The example of this is sunflower meal, by-product of sunflower seed oil industry, that is currently used as a cheap component of animal feed owing to a poor protein content. The major drawback for their application is high content of complex carbohydrates (15.27% hemicellulose, 12.54% cellulose and 5.88% lignin). Therefore, extraction and enzymatic transformation of carbohydrates (primarily xylan) represent an attractive valorization approach. Since xylan extraction from the plant cell wall is highly restricted by lignin presence, different alkaline extraction methods have been proposed. This work is focused on xylan extraction using ethanol extraction and subsequent delignification approach. The ethanol extraction step yielded fraction rich in simple sugars, polyphenols (predominantly chlorogenic acid) and colorants that proved to have great prebiotic potential for application in skin products enabling proliferation of beneficial skin commensal and suppressing the pathogen one. Introduction of additional delignification step enabled high xylan yields (91.35%). This fraction was subjected to the enzymatic treatment using commercial xylanase (ROHALASE® SEP-VISCO) to obtain xylo-oligosaccharides (XOS), compounds that comprise of 2–10 xylose residues linked via β-1,4-glycosidic bonds with high prebiotic activity and excellent application properties (high temperature and pH stability, good sweetening power and low caloric value) for food industry. As result of the sunflower meal xylan treatment under optimum reaction conditions (60°C, pH6 and enzyme concentration of 0.5% per substrate), XOS with a polymerization degree of 2–6 were produced (with predominance of XOS2), without xylose generation. The prebiotic activity of the obtained XOS fraction was confirmed by fermentation with beneficial microbial cultures and pathogenic gut microbiota representative. Therefore, it was shown that significant portion of meal can be converted to added-value functional ingredients, while remaining product retains higher protein content.", publisher = "Belgrade : University, Faculty of Geography", journal = "Book of Abstracts / International Scientific Conference Green Agenda for Western Balkans, Belgrade, June 20-22, 2023", title = "Valorization of sunflower meal toward the production of emerging prebiotics", pages = "36", url = "https://hdl.handle.net/21.15107/rcub_technorep_6842" }
Veljković, M., Simović, M., Banjanac, K., Petrov Ivanković, A., Nikolić, V.,& Bezbradica, D.. (2023). Valorization of sunflower meal toward the production of emerging prebiotics. in Book of Abstracts / International Scientific Conference Green Agenda for Western Balkans, Belgrade, June 20-22, 2023 Belgrade : University, Faculty of Geography., 36. https://hdl.handle.net/21.15107/rcub_technorep_6842
Veljković M, Simović M, Banjanac K, Petrov Ivanković A, Nikolić V, Bezbradica D. Valorization of sunflower meal toward the production of emerging prebiotics. in Book of Abstracts / International Scientific Conference Green Agenda for Western Balkans, Belgrade, June 20-22, 2023. 2023;:36. https://hdl.handle.net/21.15107/rcub_technorep_6842 .
Veljković, Milica, Simović, Milica, Banjanac, Katarina, Petrov Ivanković, Anja, Nikolić, Valentina, Bezbradica, Dejan, "Valorization of sunflower meal toward the production of emerging prebiotics" in Book of Abstracts / International Scientific Conference Green Agenda for Western Balkans, Belgrade, June 20-22, 2023 (2023):36, https://hdl.handle.net/21.15107/rcub_technorep_6842 .