NANOFILTRATION AS A TOOL FOR HIGH-YIELD PURIFICATION OF DIETARY OLIGOSACCHARIDES
Само за регистроване кориснике
2023
Аутори
Veljković, MilicaSimović, Milica
Banjanac, Katarina
Petrov Ivanković, Anja
Ćorović, Marija
Bezbradica, Dejan
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Fructo-oligosaccharides (FOS) are linear bioactive molecules consisting of one
terminal glucose and other fructose units and belong to the group of prebiotics with
scientifically proven benefits for human health. Physiological active compounds like
trisaccharides (FOS3), tetrasaccharides (FOS4) and pentasaccharides (FOS5) can be
produced from sucrose using enzyme-fructosyltransferase. The reaction mixture obtained in
this way contains, in addition to the desired prebiotics, glucose, fructose and an unreacted
amount of sucrose. Direct incorporation in products of food and pharmaceutical origin is
limited due to the presence of unwanted carbohydrates (mono- and disaccharide), and to
increase the functional value of the mixture, the attention was focused on their removal. In
this regard, membrane separation techniques present a very suitable solution for obtaining a
product with a higher FOS proportion. This study aimed to determine the optimal conditions
for purification proce...ss of produced FOS mixture using nanofiltration membrane modules.
Therefore, the influence of membranes with different MWCO (300-500Da and 600-800Da),
temperature (20-45°C) and carbohydrate concentration in feed solution (1-5%) at a constant
flow rate of 22 mL/min was investigated. From the initial mixture containing 60.2% FOS,
29.7% monosaccharides and 10.1% sucrose, after purification process under determined
optimal conditions (membrane 300-500Da, temperature 35°C and carbohydrate concentration
3%), the product with 88.8% FOS, 10.0% sucrose and 1.2% monosaccharides in total
carbohydrates was obtained. Purification yield was greater than 95% and the rejection
coefficients for glucose, fructose, sucrose, FOS3, FOS4 and FOS5 were 0.17, 0.20, 0.95,
0.99, 1.00 and 1.00, respectively. Based on the calculated purification factor, it can be
concluded that the initial mixture is refined 1.47 times. This result suggests a significant
increase in the purity of the final mixture, which can be used as a sugar substitute or addition
to numerous products that would be suitable for all consumers, including those suffering from
diabetes.
Кључне речи:
fructo-oligosaccharides / prebiotics / enzyme synthesis / optimization of nanofiltration process / membrane separation technologyИзвор:
Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade, 2023, 86-Издавач:
- Belgrade : University, Faculty of Technology and Metallurgy
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200287 (Иновациони центар Технолошко-металуршког факултета у Београду доо) (RS-MESTD-inst-2020-200287)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200135 (Универзитет у Београду, Технолошко-металуршки факултет) (RS-MESTD-inst-2020-200135)
- PrIntPrEnzy - Prebiotics for functional food and bioactive cosmetics produced in intensified enzymatic processes (RS-ScienceFundRS-Ideje-7750109)
- Horizon Europe 2021-2027 research and innovation programme under grant agreement ID 101060130 (TwinPrebioEnz)
Колекције
Институција/група
Inovacioni centarTY - CONF AU - Veljković, Milica AU - Simović, Milica AU - Banjanac, Katarina AU - Petrov Ivanković, Anja AU - Ćorović, Marija AU - Bezbradica, Dejan PY - 2023 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6962 AB - Fructo-oligosaccharides (FOS) are linear bioactive molecules consisting of one terminal glucose and other fructose units and belong to the group of prebiotics with scientifically proven benefits for human health. Physiological active compounds like trisaccharides (FOS3), tetrasaccharides (FOS4) and pentasaccharides (FOS5) can be produced from sucrose using enzyme-fructosyltransferase. The reaction mixture obtained in this way contains, in addition to the desired prebiotics, glucose, fructose and an unreacted amount of sucrose. Direct incorporation in products of food and pharmaceutical origin is limited due to the presence of unwanted carbohydrates (mono- and disaccharide), and to increase the functional value of the mixture, the attention was focused on their removal. In this regard, membrane separation techniques present a very suitable solution for obtaining a product with a higher FOS proportion. This study aimed to determine the optimal conditions for purification process of produced FOS mixture using nanofiltration membrane modules. Therefore, the influence of membranes with different MWCO (300-500Da and 600-800Da), temperature (20-45°C) and carbohydrate concentration in feed solution (1-5%) at a constant flow rate of 22 mL/min was investigated. From the initial mixture containing 60.2% FOS, 29.7% monosaccharides and 10.1% sucrose, after purification process under determined optimal conditions (membrane 300-500Da, temperature 35°C and carbohydrate concentration 3%), the product with 88.8% FOS, 10.0% sucrose and 1.2% monosaccharides in total carbohydrates was obtained. Purification yield was greater than 95% and the rejection coefficients for glucose, fructose, sucrose, FOS3, FOS4 and FOS5 were 0.17, 0.20, 0.95, 0.99, 1.00 and 1.00, respectively. Based on the calculated purification factor, it can be concluded that the initial mixture is refined 1.47 times. This result suggests a significant increase in the purity of the final mixture, which can be used as a sugar substitute or addition to numerous products that would be suitable for all consumers, including those suffering from diabetes. PB - Belgrade : University, Faculty of Technology and Metallurgy C3 - Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade T1 - NANOFILTRATION AS A TOOL FOR HIGH-YIELD PURIFICATION OF DIETARY OLIGOSACCHARIDES SP - 86 UR - https://hdl.handle.net/21.15107/rcub_technorep_6962 ER -
@conference{ author = "Veljković, Milica and Simović, Milica and Banjanac, Katarina and Petrov Ivanković, Anja and Ćorović, Marija and Bezbradica, Dejan", year = "2023", abstract = "Fructo-oligosaccharides (FOS) are linear bioactive molecules consisting of one terminal glucose and other fructose units and belong to the group of prebiotics with scientifically proven benefits for human health. Physiological active compounds like trisaccharides (FOS3), tetrasaccharides (FOS4) and pentasaccharides (FOS5) can be produced from sucrose using enzyme-fructosyltransferase. The reaction mixture obtained in this way contains, in addition to the desired prebiotics, glucose, fructose and an unreacted amount of sucrose. Direct incorporation in products of food and pharmaceutical origin is limited due to the presence of unwanted carbohydrates (mono- and disaccharide), and to increase the functional value of the mixture, the attention was focused on their removal. In this regard, membrane separation techniques present a very suitable solution for obtaining a product with a higher FOS proportion. This study aimed to determine the optimal conditions for purification process of produced FOS mixture using nanofiltration membrane modules. Therefore, the influence of membranes with different MWCO (300-500Da and 600-800Da), temperature (20-45°C) and carbohydrate concentration in feed solution (1-5%) at a constant flow rate of 22 mL/min was investigated. From the initial mixture containing 60.2% FOS, 29.7% monosaccharides and 10.1% sucrose, after purification process under determined optimal conditions (membrane 300-500Da, temperature 35°C and carbohydrate concentration 3%), the product with 88.8% FOS, 10.0% sucrose and 1.2% monosaccharides in total carbohydrates was obtained. Purification yield was greater than 95% and the rejection coefficients for glucose, fructose, sucrose, FOS3, FOS4 and FOS5 were 0.17, 0.20, 0.95, 0.99, 1.00 and 1.00, respectively. Based on the calculated purification factor, it can be concluded that the initial mixture is refined 1.47 times. This result suggests a significant increase in the purity of the final mixture, which can be used as a sugar substitute or addition to numerous products that would be suitable for all consumers, including those suffering from diabetes.", publisher = "Belgrade : University, Faculty of Technology and Metallurgy", journal = "Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade", title = "NANOFILTRATION AS A TOOL FOR HIGH-YIELD PURIFICATION OF DIETARY OLIGOSACCHARIDES", pages = "86", url = "https://hdl.handle.net/21.15107/rcub_technorep_6962" }
Veljković, M., Simović, M., Banjanac, K., Petrov Ivanković, A., Ćorović, M.,& Bezbradica, D.. (2023). NANOFILTRATION AS A TOOL FOR HIGH-YIELD PURIFICATION OF DIETARY OLIGOSACCHARIDES. in Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade Belgrade : University, Faculty of Technology and Metallurgy., 86. https://hdl.handle.net/21.15107/rcub_technorep_6962
Veljković M, Simović M, Banjanac K, Petrov Ivanković A, Ćorović M, Bezbradica D. NANOFILTRATION AS A TOOL FOR HIGH-YIELD PURIFICATION OF DIETARY OLIGOSACCHARIDES. in Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade. 2023;:86. https://hdl.handle.net/21.15107/rcub_technorep_6962 .
Veljković, Milica, Simović, Milica, Banjanac, Katarina, Petrov Ivanković, Anja, Ćorović, Marija, Bezbradica, Dejan, "NANOFILTRATION AS A TOOL FOR HIGH-YIELD PURIFICATION OF DIETARY OLIGOSACCHARIDES" in Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade (2023):86, https://hdl.handle.net/21.15107/rcub_technorep_6962 .