Alginate is one of the mostly used hydrogels for cell entrapment aimed for applications in food industry, environmental engineering, pharmacy and biomedicine. One of the major parameters affecting cell viability and activity is cell distribution inside the immobilization matrix. In addition, changes in cell distribution over the cultivation time could indicate mass transfer limitations, favorable local environments or cell differentiation. In this study, immobilization and distribution of brewing yeast in alginate microbeads were investigated as a model system of colony forming cell growth. Cell distributions were attained by image analysis of histological cross-sections of microbeads used in beer fermentation. A mathematical model based on cellular automata approach was developed for three-dimensional simulations of cell arrangement over the fermentation time.
TY - JOUR
AU - Obradović, B.
AU - Bugarski, Branko
AU - Todosijević, Zoran
AU - Nedović, Viktor
AU - Bugarski, Diana
AU - Vunjak-Novaković, Gordana
PY - 2005
UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/713
AB - Alginate is one of the mostly used hydrogels for cell entrapment aimed for applications in food industry, environmental engineering, pharmacy and biomedicine. One of the major parameters affecting cell viability and activity is cell distribution inside the immobilization matrix. In addition, changes in cell distribution over the cultivation time could indicate mass transfer limitations, favorable local environments or cell differentiation. In this study, immobilization and distribution of brewing yeast in alginate microbeads were investigated as a model system of colony forming cell growth. Cell distributions were attained by image analysis of histological cross-sections of microbeads used in beer fermentation. A mathematical model based on cellular automata approach was developed for three-dimensional simulations of cell arrangement over the fermentation time.
PB - 6th Conference of the Yugoslav Materials Research Society, YUCOMAT VI: Current Research in Advanced Materials and Processes
T2 - Materials Science Forum
T1 - Mathematical modeling of cell distribution in alginate microbeads
EP - 536
SP - 531
VL - 494
DO - 10.4028/0-87849-971-7.531
ER -
@article{
author = "Obradović, B. and Bugarski, Branko and Todosijević, Zoran and Nedović, Viktor and Bugarski, Diana and Vunjak-Novaković, Gordana",
year = "2005",
abstract = "Alginate is one of the mostly used hydrogels for cell entrapment aimed for applications in food industry, environmental engineering, pharmacy and biomedicine. One of the major parameters affecting cell viability and activity is cell distribution inside the immobilization matrix. In addition, changes in cell distribution over the cultivation time could indicate mass transfer limitations, favorable local environments or cell differentiation. In this study, immobilization and distribution of brewing yeast in alginate microbeads were investigated as a model system of colony forming cell growth. Cell distributions were attained by image analysis of histological cross-sections of microbeads used in beer fermentation. A mathematical model based on cellular automata approach was developed for three-dimensional simulations of cell arrangement over the fermentation time.",
publisher = "6th Conference of the Yugoslav Materials Research Society, YUCOMAT VI: Current Research in Advanced Materials and Processes",
journal = "Materials Science Forum",
title = "Mathematical modeling of cell distribution in alginate microbeads",
pages = "536-531",
volume = "494",
doi = "10.4028/0-87849-971-7.531"
}
Obradović, B., Bugarski, B., Todosijević, Z., Nedović, V., Bugarski, D.,& Vunjak-Novaković, G.. (2005). Mathematical modeling of cell distribution in alginate microbeads. in Materials Science Forum
6th Conference of the Yugoslav Materials Research Society, YUCOMAT VI: Current Research in Advanced Materials and Processes., 494, 531-536.
https://doi.org/10.4028/0-87849-971-7.531
Obradović B, Bugarski B, Todosijević Z, Nedović V, Bugarski D, Vunjak-Novaković G. Mathematical modeling of cell distribution in alginate microbeads. in Materials Science Forum. 2005;494:531-536.
doi:10.4028/0-87849-971-7.531 .
Obradović, B., Bugarski, Branko, Todosijević, Zoran, Nedović, Viktor, Bugarski, Diana, Vunjak-Novaković, Gordana, "Mathematical modeling of cell distribution in alginate microbeads" in Materials Science Forum, 494 (2005):531-536,
https://doi.org/10.4028/0-87849-971-7.531 . .
