Ispitivanje stabilnosti anizomicina metodom tečne hromatografije sa tandem masenom spektrometrijom
Investigation of anisomycin stability using liquid chromatography coupled to tandem mass spectrometry
Само за регистроване кориснике
2016
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
U cilju poboljšanja kvaliteta leka i unapređenja sigurnosti njegovog korišćenja, potrebno je praćenje stabilnosti i određivanje degradacionih proizvoda koji mogu nastati tokom proizvodnje, rukovanja ili čuvanja leka. Cilj ovog rada je ispitivanje stabilnosti i praćenje degradacije višefun-kcionalnog leka anizomicina. Stabilnost anizomicina je ispitivana sprovođenjem studija forsirane degradacije, koje obuhvataju ispitivanje osetljivosti leka na hidrolizu (kiselu, baznu i neutralnu), oksidaciju, svetlost (vidljivu i UV) i povišenu temperaturu, u skladu sa ICH (eng. International Conference on Harmonization) smernicama. Za praćenje degradacije leka i identifikaciju degradacionih prozvoda korišćena je metoda tečne hromatografije sa tandem masenom spek-trometrijom. Pokazano je da je anizomicin stabilan u prisustvu oksidacionog sredstva i svetlosti, umereno stabilan u kiseloj i neutralnoj sredini, jako nestabilan u baznoj sredini i termo-degradabilan. Deacetilanizomicin je identifikovan kao... glavni proizvod degradacije anisomicina.
In order to improve the quality of a drug and its safety usage, it is necessary to monitor stability
of a drug and to determine degradation products that may form during production, usage and
storage. Aim of this work was to investigate stability and monitor degradation of the
multifunctional drug anisomycin. Stability of anisomycin was studied by performing force
degradation that includes investigation of susceptibility of the drug to hydrolysis (acidic, alkaline
and neutral), oxidation, light (visible and UV) and temperature, in accordance with ICH
(International Conference on Harmonization) guidelines. Liquid chromatography coupled to
tandem mass spectrometry was used for investigation of the drug degradation and identification
of the degradation products. It was shown that anisomycin was stable in the presence of
oxidizing agent and light, moderately stable under acidic and neutral conditions, very unstable
under alkaline conditions and thermodegradable. Deacetylanisomyci...n was identified as the
principal degradation product of anisomycin.
Кључне речи:
Lek / Stabilnost / Degardacioni proizvodi / Tečna hromatografija–tandem masena spektrometrijaИзвор:
Book of Abstracts / Fourth Conference of Young Chemists of Serbia, Belgrade, Serbia, November 5, 2016, 2016, 30-Издавач:
- Belgrade : Serbian Chemical Society
Финансирање / пројекти:
- Развој и примена метода и материјала за мониторинг нових загађујућих и токсичних органских материја и тешких метала (RS-MESTD-Basic Research (BR or ON)-172007)
Колекције
Институција/група
Inovacioni centarTY - CONF AU - Tolić, Ljiljana AU - Grujić, Svetlana AU - Laušević, Mila PY - 2016 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7256 AB - U cilju poboljšanja kvaliteta leka i unapređenja sigurnosti njegovog korišćenja, potrebno je praćenje stabilnosti i određivanje degradacionih proizvoda koji mogu nastati tokom proizvodnje, rukovanja ili čuvanja leka. Cilj ovog rada je ispitivanje stabilnosti i praćenje degradacije višefun-kcionalnog leka anizomicina. Stabilnost anizomicina je ispitivana sprovođenjem studija forsirane degradacije, koje obuhvataju ispitivanje osetljivosti leka na hidrolizu (kiselu, baznu i neutralnu), oksidaciju, svetlost (vidljivu i UV) i povišenu temperaturu, u skladu sa ICH (eng. International Conference on Harmonization) smernicama. Za praćenje degradacije leka i identifikaciju degradacionih prozvoda korišćena je metoda tečne hromatografije sa tandem masenom spek-trometrijom. Pokazano je da je anizomicin stabilan u prisustvu oksidacionog sredstva i svetlosti, umereno stabilan u kiseloj i neutralnoj sredini, jako nestabilan u baznoj sredini i termo-degradabilan. Deacetilanizomicin je identifikovan kao glavni proizvod degradacije anisomicina. AB - In order to improve the quality of a drug and its safety usage, it is necessary to monitor stability of a drug and to determine degradation products that may form during production, usage and storage. Aim of this work was to investigate stability and monitor degradation of the multifunctional drug anisomycin. Stability of anisomycin was studied by performing force degradation that includes investigation of susceptibility of the drug to hydrolysis (acidic, alkaline and neutral), oxidation, light (visible and UV) and temperature, in accordance with ICH (International Conference on Harmonization) guidelines. Liquid chromatography coupled to tandem mass spectrometry was used for investigation of the drug degradation and identification of the degradation products. It was shown that anisomycin was stable in the presence of oxidizing agent and light, moderately stable under acidic and neutral conditions, very unstable under alkaline conditions and thermodegradable. Deacetylanisomycin was identified as the principal degradation product of anisomycin. PB - Belgrade : Serbian Chemical Society C3 - Book of Abstracts / Fourth Conference of Young Chemists of Serbia, Belgrade, Serbia, November 5, 2016 T1 - Ispitivanje stabilnosti anizomicina metodom tečne hromatografije sa tandem masenom spektrometrijom T1 - Investigation of anisomycin stability using liquid chromatography coupled to tandem mass spectrometry SP - 30 UR - https://hdl.handle.net/21.15107/rcub_technorep_7256 ER -
@conference{ author = "Tolić, Ljiljana and Grujić, Svetlana and Laušević, Mila", year = "2016", abstract = "U cilju poboljšanja kvaliteta leka i unapređenja sigurnosti njegovog korišćenja, potrebno je praćenje stabilnosti i određivanje degradacionih proizvoda koji mogu nastati tokom proizvodnje, rukovanja ili čuvanja leka. Cilj ovog rada je ispitivanje stabilnosti i praćenje degradacije višefun-kcionalnog leka anizomicina. Stabilnost anizomicina je ispitivana sprovođenjem studija forsirane degradacije, koje obuhvataju ispitivanje osetljivosti leka na hidrolizu (kiselu, baznu i neutralnu), oksidaciju, svetlost (vidljivu i UV) i povišenu temperaturu, u skladu sa ICH (eng. International Conference on Harmonization) smernicama. Za praćenje degradacije leka i identifikaciju degradacionih prozvoda korišćena je metoda tečne hromatografije sa tandem masenom spek-trometrijom. Pokazano je da je anizomicin stabilan u prisustvu oksidacionog sredstva i svetlosti, umereno stabilan u kiseloj i neutralnoj sredini, jako nestabilan u baznoj sredini i termo-degradabilan. Deacetilanizomicin je identifikovan kao glavni proizvod degradacije anisomicina., In order to improve the quality of a drug and its safety usage, it is necessary to monitor stability of a drug and to determine degradation products that may form during production, usage and storage. Aim of this work was to investigate stability and monitor degradation of the multifunctional drug anisomycin. Stability of anisomycin was studied by performing force degradation that includes investigation of susceptibility of the drug to hydrolysis (acidic, alkaline and neutral), oxidation, light (visible and UV) and temperature, in accordance with ICH (International Conference on Harmonization) guidelines. Liquid chromatography coupled to tandem mass spectrometry was used for investigation of the drug degradation and identification of the degradation products. It was shown that anisomycin was stable in the presence of oxidizing agent and light, moderately stable under acidic and neutral conditions, very unstable under alkaline conditions and thermodegradable. Deacetylanisomycin was identified as the principal degradation product of anisomycin.", publisher = "Belgrade : Serbian Chemical Society", journal = "Book of Abstracts / Fourth Conference of Young Chemists of Serbia, Belgrade, Serbia, November 5, 2016", title = "Ispitivanje stabilnosti anizomicina metodom tečne hromatografije sa tandem masenom spektrometrijom, Investigation of anisomycin stability using liquid chromatography coupled to tandem mass spectrometry", pages = "30", url = "https://hdl.handle.net/21.15107/rcub_technorep_7256" }
Tolić, L., Grujić, S.,& Laušević, M.. (2016). Ispitivanje stabilnosti anizomicina metodom tečne hromatografije sa tandem masenom spektrometrijom. in Book of Abstracts / Fourth Conference of Young Chemists of Serbia, Belgrade, Serbia, November 5, 2016 Belgrade : Serbian Chemical Society., 30. https://hdl.handle.net/21.15107/rcub_technorep_7256
Tolić L, Grujić S, Laušević M. Ispitivanje stabilnosti anizomicina metodom tečne hromatografije sa tandem masenom spektrometrijom. in Book of Abstracts / Fourth Conference of Young Chemists of Serbia, Belgrade, Serbia, November 5, 2016. 2016;:30. https://hdl.handle.net/21.15107/rcub_technorep_7256 .
Tolić, Ljiljana, Grujić, Svetlana, Laušević, Mila, "Ispitivanje stabilnosti anizomicina metodom tečne hromatografije sa tandem masenom spektrometrijom" in Book of Abstracts / Fourth Conference of Young Chemists of Serbia, Belgrade, Serbia, November 5, 2016 (2016):30, https://hdl.handle.net/21.15107/rcub_technorep_7256 .