AFM studies of DNA structures extracted from adriamycin treated and non-treated Ehrlich tumor cells

2005
Authors
Avramov-Ivić, Milka
Petrović, Slobodan
Kalman, E
Milosavljević, T
Reljin, Irini S.
Reljin, Branimir D.
Bogdanović, G.

Baltić, VV
Keresztes, Z
Article (Published version)
Metadata
Show full item recordAbstract
Atomic force microscopy (AFM), a unique tool to investigate drug treatment of cancer cells, was used to analyze the anti-neoplastic activity of adriamycin by comparing DNA structures of non-treated and adriamycin-treated Ehrlich tumor cells. The non-treated cells exhibited a highly branched intact chromatin structure, related to the intensive DNA replication in cancer cells. Images from adriamycin-treated tumor cells showed that the DNA chains were broken and the chromatin structure had been destroyed. Possible explanations for these effects of adriamycin are considered: breakage of hydrogen bonding, oxidation and intercalation effects, as well as the poisoning of topoisomerase enzyme. DNA fractal and multifractal analyses, performed in order to evaluate the degree of bond scission, showed that the treated DNA had become more fractal compared to non-treated DNA.
Keywords:
adriamycin / Ehrlich tumor cells / atomic force microscopy / topoisomerase / fractal and multifractal analysisSource:
Journal of the Serbian Chemical Society, 2005, 70, 6, 823-831Publisher:
- Srpsko hemijsko društvo, Beograd
DOI: 10.2298/JSC0506823A
ISSN: 0352-5139
WoS: 000231057300005
Scopus: 2-s2.0-31544450291
Institution/Community
Tehnološko-metalurški fakultetTY - JOUR AU - Avramov-Ivić, Milka AU - Petrović, Slobodan AU - Kalman, E AU - Milosavljević, T AU - Reljin, Irini S. AU - Reljin, Branimir D. AU - Bogdanović, G. AU - Baltić, VV AU - Keresztes, Z PY - 2005 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/854 AB - Atomic force microscopy (AFM), a unique tool to investigate drug treatment of cancer cells, was used to analyze the anti-neoplastic activity of adriamycin by comparing DNA structures of non-treated and adriamycin-treated Ehrlich tumor cells. The non-treated cells exhibited a highly branched intact chromatin structure, related to the intensive DNA replication in cancer cells. Images from adriamycin-treated tumor cells showed that the DNA chains were broken and the chromatin structure had been destroyed. Possible explanations for these effects of adriamycin are considered: breakage of hydrogen bonding, oxidation and intercalation effects, as well as the poisoning of topoisomerase enzyme. DNA fractal and multifractal analyses, performed in order to evaluate the degree of bond scission, showed that the treated DNA had become more fractal compared to non-treated DNA. PB - Srpsko hemijsko društvo, Beograd T2 - Journal of the Serbian Chemical Society T1 - AFM studies of DNA structures extracted from adriamycin treated and non-treated Ehrlich tumor cells EP - 831 IS - 6 SP - 823 VL - 70 DO - 10.2298/JSC0506823A ER -
@article{ author = "Avramov-Ivić, Milka and Petrović, Slobodan and Kalman, E and Milosavljević, T and Reljin, Irini S. and Reljin, Branimir D. and Bogdanović, G. and Baltić, VV and Keresztes, Z", year = "2005", abstract = "Atomic force microscopy (AFM), a unique tool to investigate drug treatment of cancer cells, was used to analyze the anti-neoplastic activity of adriamycin by comparing DNA structures of non-treated and adriamycin-treated Ehrlich tumor cells. The non-treated cells exhibited a highly branched intact chromatin structure, related to the intensive DNA replication in cancer cells. Images from adriamycin-treated tumor cells showed that the DNA chains were broken and the chromatin structure had been destroyed. Possible explanations for these effects of adriamycin are considered: breakage of hydrogen bonding, oxidation and intercalation effects, as well as the poisoning of topoisomerase enzyme. DNA fractal and multifractal analyses, performed in order to evaluate the degree of bond scission, showed that the treated DNA had become more fractal compared to non-treated DNA.", publisher = "Srpsko hemijsko društvo, Beograd", journal = "Journal of the Serbian Chemical Society", title = "AFM studies of DNA structures extracted from adriamycin treated and non-treated Ehrlich tumor cells", pages = "831-823", number = "6", volume = "70", doi = "10.2298/JSC0506823A" }
Avramov-Ivić, M., Petrović, S., Kalman, E., Milosavljević, T., Reljin, I. S., Reljin, B. D., Bogdanović, G., Baltić, V.,& Keresztes, Z.. (2005). AFM studies of DNA structures extracted from adriamycin treated and non-treated Ehrlich tumor cells. in Journal of the Serbian Chemical Society Srpsko hemijsko društvo, Beograd., 70(6), 823-831. https://doi.org/10.2298/JSC0506823A
Avramov-Ivić M, Petrović S, Kalman E, Milosavljević T, Reljin IS, Reljin BD, Bogdanović G, Baltić V, Keresztes Z. AFM studies of DNA structures extracted from adriamycin treated and non-treated Ehrlich tumor cells. in Journal of the Serbian Chemical Society. 2005;70(6):823-831. doi:10.2298/JSC0506823A .
Avramov-Ivić, Milka, Petrović, Slobodan, Kalman, E, Milosavljević, T, Reljin, Irini S., Reljin, Branimir D., Bogdanović, G., Baltić, VV, Keresztes, Z, "AFM studies of DNA structures extracted from adriamycin treated and non-treated Ehrlich tumor cells" in Journal of the Serbian Chemical Society, 70, no. 6 (2005):823-831, https://doi.org/10.2298/JSC0506823A . .