Atomic force microscopy (AFM), a unique tool to investigate drug treatment of cancer cells, was used to analyze the anti-neoplastic activity of adriamycin by comparing DNA structures of non-treated and adriamycin-treated Ehrlich tumor cells. The non-treated cells exhibited a highly branched intact chromatin structure, related to the intensive DNA replication in cancer cells. Images from adriamycin-treated tumor cells showed that the DNA chains were broken and the chromatin structure had been destroyed. Possible explanations for these effects of adriamycin are considered: breakage of hydrogen bonding, oxidation and intercalation effects, as well as the poisoning of topoisomerase enzyme. DNA fractal and multifractal analyses, performed in order to evaluate the degree of bond scission, showed that the treated DNA had become more fractal compared to non-treated DNA.
Keywords:
adriamycin / Ehrlich tumor cells / atomic force microscopy / topoisomerase / fractal and multifractal analysis
Source:
Journal of the Serbian Chemical Society, 2005, 70, 6, 823-831
TY - JOUR
AU - Avramov-Ivić, Milka
AU - Petrović, Slobodan
AU - Kalman, E
AU - Milosavljević, T
AU - Reljin, Irini S.
AU - Reljin, Branimir D.
AU - Bogdanović, G.
AU - Baltić, VV
AU - Keresztes, Z
PY - 2005
UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/854
AB - Atomic force microscopy (AFM), a unique tool to investigate drug treatment of cancer cells, was used to analyze the anti-neoplastic activity of adriamycin by comparing DNA structures of non-treated and adriamycin-treated Ehrlich tumor cells. The non-treated cells exhibited a highly branched intact chromatin structure, related to the intensive DNA replication in cancer cells. Images from adriamycin-treated tumor cells showed that the DNA chains were broken and the chromatin structure had been destroyed. Possible explanations for these effects of adriamycin are considered: breakage of hydrogen bonding, oxidation and intercalation effects, as well as the poisoning of topoisomerase enzyme. DNA fractal and multifractal analyses, performed in order to evaluate the degree of bond scission, showed that the treated DNA had become more fractal compared to non-treated DNA.
PB - Srpsko hemijsko društvo, Beograd
T2 - Journal of the Serbian Chemical Society
T1 - AFM studies of DNA structures extracted from adriamycin treated and non-treated Ehrlich tumor cells
EP - 831
IS - 6
SP - 823
VL - 70
DO - 10.2298/JSC0506823A
ER -
@article{
author = "Avramov-Ivić, Milka and Petrović, Slobodan and Kalman, E and Milosavljević, T and Reljin, Irini S. and Reljin, Branimir D. and Bogdanović, G. and Baltić, VV and Keresztes, Z",
year = "2005",
abstract = "Atomic force microscopy (AFM), a unique tool to investigate drug treatment of cancer cells, was used to analyze the anti-neoplastic activity of adriamycin by comparing DNA structures of non-treated and adriamycin-treated Ehrlich tumor cells. The non-treated cells exhibited a highly branched intact chromatin structure, related to the intensive DNA replication in cancer cells. Images from adriamycin-treated tumor cells showed that the DNA chains were broken and the chromatin structure had been destroyed. Possible explanations for these effects of adriamycin are considered: breakage of hydrogen bonding, oxidation and intercalation effects, as well as the poisoning of topoisomerase enzyme. DNA fractal and multifractal analyses, performed in order to evaluate the degree of bond scission, showed that the treated DNA had become more fractal compared to non-treated DNA.",
publisher = "Srpsko hemijsko društvo, Beograd",
journal = "Journal of the Serbian Chemical Society",
title = "AFM studies of DNA structures extracted from adriamycin treated and non-treated Ehrlich tumor cells",
pages = "831-823",
number = "6",
volume = "70",
doi = "10.2298/JSC0506823A"
}
Avramov-Ivić, M., Petrović, S., Kalman, E., Milosavljević, T., Reljin, I. S., Reljin, B. D., Bogdanović, G., Baltić, V.,& Keresztes, Z.. (2005). AFM studies of DNA structures extracted from adriamycin treated and non-treated Ehrlich tumor cells. in Journal of the Serbian Chemical Society
Srpsko hemijsko društvo, Beograd., 70(6), 823-831.
https://doi.org/10.2298/JSC0506823A
Avramov-Ivić M, Petrović S, Kalman E, Milosavljević T, Reljin IS, Reljin BD, Bogdanović G, Baltić V, Keresztes Z. AFM studies of DNA structures extracted from adriamycin treated and non-treated Ehrlich tumor cells. in Journal of the Serbian Chemical Society. 2005;70(6):823-831.
doi:10.2298/JSC0506823A .
Avramov-Ivić, Milka, Petrović, Slobodan, Kalman, E, Milosavljević, T, Reljin, Irini S., Reljin, Branimir D., Bogdanović, G., Baltić, VV, Keresztes, Z, "AFM studies of DNA structures extracted from adriamycin treated and non-treated Ehrlich tumor cells" in Journal of the Serbian Chemical Society, 70, no. 6 (2005):823-831,
https://doi.org/10.2298/JSC0506823A . .
