TY  - CHAP
AU  - Ivanović, Jasna
AU  - Milovanović, Stoja
AU  - Stamenić, Marko
AU  - Fanovich, Marija Alejandra
AU  - Jaeger, Philip
AU  - Žižović, Irena
PY  - 2014
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2566
AB  - Supercritical carbon dioxide (sc2) is known as a good solvent for a wide range of bioactive compounds from natural sources. Extraction of bioactive compounds from plant material using sc2 is a favorable technique for producing solvent-free extracts suitable for a wide range of applications in pharmaceutical, biomedical, cosmetic and food industries. Incorporation of drugs or other bioactive substances into polymeric matrices by conventional techniques is connected to some significant drawbacks (use of organic solvents, undesired substances reactions and/or degradation, low incorporation yields and heterogeneous dispersion). Supercritical solvent impregnation (SSI), and namely impregnation using sc2, has been proposed as an alternative methodology to overcome most of these problems. The main advantages of this technique are avoidance of organic solvents and possibility to work at relatively low operational temperatures and withhydrophobic drugs/substances which cannot be impregnated by aqueous solution/suspension soaking methods. Additionally, unique physical and transport properties of sc2 can be used simultaneously for impregnation of biopolymers andtailoring the chemistry and morphology thereof to obtain the desired microstructure of the final product. The present chapter is particularly aimed to demonstrate feasibility of incorporating of thyme extracts into different solid matrices by using a methodology that combines the supercritical fluid extraction (SFE) and SSI. Compared to both processes to be conducted separately, the advantage of using one single integrated process is basically to save energy and time by avoiding an intermediate decompression procedure. Representative commercial carriers with relevance for pharmaceutical, biomedical and food applications (cotton gauze, polypropylene non-woven fabrics, chitosan and starch gels, cellulose acetate and polycaprolactone) were used to test feasibility of thyme extract incorporation by the coupled SFE-SSI process. By setting the relevant parameters of each process separately, the challenge of this methodology aims to harmonize these parameters in order to increase the extraction and impregnation efficiencies as well as to obtain an even distribution of the solute within the solid matrix.
T2  - Handbook on Supercritical Fluids: Fundamentals, Properties and Applications
T1  - Application of an integrated supercritical extraction and impregnation process for incorporation of thyme extracts into different carriers
EP  - 280
SP  - 258
UR  - https://hdl.handle.net/21.15107/rcub_technorep_2566
ER  - 

@inbook{
author = "Ivanović, Jasna and Milovanović, Stoja and Stamenić, Marko and Fanovich, Marija Alejandra and Jaeger, Philip and Žižović, Irena",
year = "2014",
abstract = "Supercritical carbon dioxide (sc2) is known as a good solvent for a wide range of bioactive compounds from natural sources. Extraction of bioactive compounds from plant material using sc2 is a favorable technique for producing solvent-free extracts suitable for a wide range of applications in pharmaceutical, biomedical, cosmetic and food industries. Incorporation of drugs or other bioactive substances into polymeric matrices by conventional techniques is connected to some significant drawbacks (use of organic solvents, undesired substances reactions and/or degradation, low incorporation yields and heterogeneous dispersion). Supercritical solvent impregnation (SSI), and namely impregnation using sc2, has been proposed as an alternative methodology to overcome most of these problems. The main advantages of this technique are avoidance of organic solvents and possibility to work at relatively low operational temperatures and withhydrophobic drugs/substances which cannot be impregnated by aqueous solution/suspension soaking methods. Additionally, unique physical and transport properties of sc2 can be used simultaneously for impregnation of biopolymers andtailoring the chemistry and morphology thereof to obtain the desired microstructure of the final product. The present chapter is particularly aimed to demonstrate feasibility of incorporating of thyme extracts into different solid matrices by using a methodology that combines the supercritical fluid extraction (SFE) and SSI. Compared to both processes to be conducted separately, the advantage of using one single integrated process is basically to save energy and time by avoiding an intermediate decompression procedure. Representative commercial carriers with relevance for pharmaceutical, biomedical and food applications (cotton gauze, polypropylene non-woven fabrics, chitosan and starch gels, cellulose acetate and polycaprolactone) were used to test feasibility of thyme extract incorporation by the coupled SFE-SSI process. By setting the relevant parameters of each process separately, the challenge of this methodology aims to harmonize these parameters in order to increase the extraction and impregnation efficiencies as well as to obtain an even distribution of the solute within the solid matrix.",
journal = "Handbook on Supercritical Fluids: Fundamentals, Properties and Applications",
booktitle = "Application of an integrated supercritical extraction and impregnation process for incorporation of thyme extracts into different carriers",
pages = "280-258",
url = "https://hdl.handle.net/21.15107/rcub_technorep_2566"
}

Ivanović, J., Milovanović, S., Stamenić, M., Fanovich, M. A., Jaeger, P.,& Žižović, I.. (2014). Application of an integrated supercritical extraction and impregnation process for incorporation of thyme extracts into different carriers. in Handbook on Supercritical Fluids: Fundamentals, Properties and Applications, 258-280.
https://hdl.handle.net/21.15107/rcub_technorep_2566

Ivanović J, Milovanović S, Stamenić M, Fanovich MA, Jaeger P, Žižović I. Application of an integrated supercritical extraction and impregnation process for incorporation of thyme extracts into different carriers. in Handbook on Supercritical Fluids: Fundamentals, Properties and Applications. 2014;:258-280.
https://hdl.handle.net/21.15107/rcub_technorep_2566 .

Ivanović, Jasna, Milovanović, Stoja, Stamenić, Marko, Fanovich, Marija Alejandra, Jaeger, Philip, Žižović, Irena, "Application of an integrated supercritical extraction and impregnation process for incorporation of thyme extracts into different carriers" in Handbook on Supercritical Fluids: Fundamentals, Properties and Applications (2014):258-280,
https://hdl.handle.net/21.15107/rcub_technorep_2566 .

TY  - JOUR
AU  - Fanovich, M.A.
AU  - Ivanović, J.
AU  - Mišić, D.
AU  - Alvarez, M.V.
AU  - Jaeger, P.
AU  - Žižović, Irena
AU  - Eggers, Rudolf
PY  - 2013
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5698
AB  - The present study is aimed to develop a process for production of functionalized scaffolds impregnated with natural compounds extracted from Patagonian Usnea lichen. A setup for an integrated supercritical CO2 extraction of natural compounds with posterior impregnation on solid matrices (polycaprolactone, PCL) is developed and presented here. In order to establish optimized operating conditions, supercritical extraction of Usnea as well as sorption kinetics and resulting material properties have been studied separately first. Usnea extracts isolated by supercritical carbon dioxide at 30 MPa and 40 C have shown strong antibacterial activity with values of the minimum inhibitory concentration (MIC) ranging from less than 1.25 μg/mL to 320 μg/mL against Listeria innocua and Methicillin-resistant Staphylococcus (MRS) strains. Useful scaffolds of PCL for tissue engineering containing a porous structure with pore diameters between 150 and 340 μm can be obtained when PCL is exposed to carbon dioxide at 35 C and 15 MPa. The degree of crystallinity of functionalized PCL was shown to be influenced by the incorporated antibacterial agent. The presented results showed that the impregnated PCL samples are promising bactericidal compounds against L. innocua. Screening of antibacterial activity of functionalized PCL against a Methicillin-resistant Staphylococcus aureus (MRSA) strain showed a higher activity when a low bacterial inoculum level (2 × 104 Colony-forming Units/mL [CFU/mL]) was assayed.
PB  - Elsevier
T2  - Journal of Supercritical Fluids
T1  - Development of polycaprolactone scaffold with antibacterial activity by an integrated supercritical extraction and impregnation process
EP  - 53
SP  - 42
VL  - 78
DO  - 10.1016/j.supflu.2013.03.017
ER  - 

@article{
author = "Fanovich, M.A. and Ivanović, J. and Mišić, D. and Alvarez, M.V. and Jaeger, P. and Žižović, Irena and Eggers, Rudolf",
year = "2013",
abstract = "The present study is aimed to develop a process for production of functionalized scaffolds impregnated with natural compounds extracted from Patagonian Usnea lichen. A setup for an integrated supercritical CO2 extraction of natural compounds with posterior impregnation on solid matrices (polycaprolactone, PCL) is developed and presented here. In order to establish optimized operating conditions, supercritical extraction of Usnea as well as sorption kinetics and resulting material properties have been studied separately first. Usnea extracts isolated by supercritical carbon dioxide at 30 MPa and 40 C have shown strong antibacterial activity with values of the minimum inhibitory concentration (MIC) ranging from less than 1.25 μg/mL to 320 μg/mL against Listeria innocua and Methicillin-resistant Staphylococcus (MRS) strains. Useful scaffolds of PCL for tissue engineering containing a porous structure with pore diameters between 150 and 340 μm can be obtained when PCL is exposed to carbon dioxide at 35 C and 15 MPa. The degree of crystallinity of functionalized PCL was shown to be influenced by the incorporated antibacterial agent. The presented results showed that the impregnated PCL samples are promising bactericidal compounds against L. innocua. Screening of antibacterial activity of functionalized PCL against a Methicillin-resistant Staphylococcus aureus (MRSA) strain showed a higher activity when a low bacterial inoculum level (2 × 104 Colony-forming Units/mL [CFU/mL]) was assayed.",
publisher = "Elsevier",
journal = "Journal of Supercritical Fluids",
title = "Development of polycaprolactone scaffold with antibacterial activity by an integrated supercritical extraction and impregnation process",
pages = "53-42",
volume = "78",
doi = "10.1016/j.supflu.2013.03.017"
}

Fanovich, M.A., Ivanović, J., Mišić, D., Alvarez, M.V., Jaeger, P., Žižović, I.,& Eggers, R.. (2013). Development of polycaprolactone scaffold with antibacterial activity by an integrated supercritical extraction and impregnation process. in Journal of Supercritical Fluids
Elsevier., 78, 42-53.
https://doi.org/10.1016/j.supflu.2013.03.017

Fanovich M, Ivanović J, Mišić D, Alvarez M, Jaeger P, Žižović I, Eggers R. Development of polycaprolactone scaffold with antibacterial activity by an integrated supercritical extraction and impregnation process. in Journal of Supercritical Fluids. 2013;78:42-53.
doi:10.1016/j.supflu.2013.03.017 .

Fanovich, M.A., Ivanović, J., Mišić, D., Alvarez, M.V., Jaeger, P., Žižović, Irena, Eggers, Rudolf, "Development of polycaprolactone scaffold with antibacterial activity by an integrated supercritical extraction and impregnation process" in Journal of Supercritical Fluids, 78 (2013):42-53,
https://doi.org/10.1016/j.supflu.2013.03.017 . .