Development of polycaprolactone scaffold with antibacterial activity by an integrated supercritical extraction and impregnation process
Само за регистроване кориснике
2013
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The present study is aimed to develop a process for production of functionalized scaffolds impregnated with natural compounds extracted from Patagonian Usnea lichen. A setup for an integrated supercritical CO2 extraction of natural compounds with posterior impregnation on solid matrices (polycaprolactone, PCL) is developed and presented here. In order to establish optimized operating conditions, supercritical extraction of Usnea as well as sorption kinetics and resulting material properties have been studied separately first. Usnea extracts isolated by supercritical carbon dioxide at 30 MPa and 40 C have shown strong antibacterial activity with values of the minimum inhibitory concentration (MIC) ranging from less than 1.25 μg/mL to 320 μg/mL against Listeria innocua and Methicillin-resistant Staphylococcus (MRS) strains. Useful scaffolds of PCL for tissue engineering containing a porous structure with pore diameters between 150 and 340 μm can be obtained when PCL is exposed to carbon ...dioxide at 35 C and 15 MPa. The degree of crystallinity of functionalized PCL was shown to be influenced by the incorporated antibacterial agent. The presented results showed that the impregnated PCL samples are promising bactericidal compounds against L. innocua. Screening of antibacterial activity of functionalized PCL against a Methicillin-resistant Staphylococcus aureus (MRSA) strain showed a higher activity when a low bacterial inoculum level (2 × 104 Colony-forming Units/mL [CFU/mL]) was assayed.
Кључне речи:
Antibacterial properties / Polycaprolactone scaffold / Supercritical extraction impregnation / Usnea extractИзвор:
Journal of Supercritical Fluids, 2013, 78, 42-53Издавач:
- Elsevier
Финансирање / пројекти:
- Функционални физиолошки активни биљни материјали са додатом вредношћу за примену у фармацеутској и прехрамбеној индустрији (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-45017)
DOI: 10.1016/j.supflu.2013.03.017
ISSN: 0896-8446
WoS: 000319854000007
Scopus: 2-s2.0-84876222754
Институција/група
Tehnološko-metalurški fakultetTY - JOUR AU - Fanovich, M.A. AU - Ivanović, J. AU - Mišić, D. AU - Alvarez, M.V. AU - Jaeger, P. AU - Žižović, Irena AU - Eggers, Rudolf PY - 2013 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5698 AB - The present study is aimed to develop a process for production of functionalized scaffolds impregnated with natural compounds extracted from Patagonian Usnea lichen. A setup for an integrated supercritical CO2 extraction of natural compounds with posterior impregnation on solid matrices (polycaprolactone, PCL) is developed and presented here. In order to establish optimized operating conditions, supercritical extraction of Usnea as well as sorption kinetics and resulting material properties have been studied separately first. Usnea extracts isolated by supercritical carbon dioxide at 30 MPa and 40 C have shown strong antibacterial activity with values of the minimum inhibitory concentration (MIC) ranging from less than 1.25 μg/mL to 320 μg/mL against Listeria innocua and Methicillin-resistant Staphylococcus (MRS) strains. Useful scaffolds of PCL for tissue engineering containing a porous structure with pore diameters between 150 and 340 μm can be obtained when PCL is exposed to carbon dioxide at 35 C and 15 MPa. The degree of crystallinity of functionalized PCL was shown to be influenced by the incorporated antibacterial agent. The presented results showed that the impregnated PCL samples are promising bactericidal compounds against L. innocua. Screening of antibacterial activity of functionalized PCL against a Methicillin-resistant Staphylococcus aureus (MRSA) strain showed a higher activity when a low bacterial inoculum level (2 × 104 Colony-forming Units/mL [CFU/mL]) was assayed. PB - Elsevier T2 - Journal of Supercritical Fluids T1 - Development of polycaprolactone scaffold with antibacterial activity by an integrated supercritical extraction and impregnation process EP - 53 SP - 42 VL - 78 DO - 10.1016/j.supflu.2013.03.017 ER -
@article{ author = "Fanovich, M.A. and Ivanović, J. and Mišić, D. and Alvarez, M.V. and Jaeger, P. and Žižović, Irena and Eggers, Rudolf", year = "2013", abstract = "The present study is aimed to develop a process for production of functionalized scaffolds impregnated with natural compounds extracted from Patagonian Usnea lichen. A setup for an integrated supercritical CO2 extraction of natural compounds with posterior impregnation on solid matrices (polycaprolactone, PCL) is developed and presented here. In order to establish optimized operating conditions, supercritical extraction of Usnea as well as sorption kinetics and resulting material properties have been studied separately first. Usnea extracts isolated by supercritical carbon dioxide at 30 MPa and 40 C have shown strong antibacterial activity with values of the minimum inhibitory concentration (MIC) ranging from less than 1.25 μg/mL to 320 μg/mL against Listeria innocua and Methicillin-resistant Staphylococcus (MRS) strains. Useful scaffolds of PCL for tissue engineering containing a porous structure with pore diameters between 150 and 340 μm can be obtained when PCL is exposed to carbon dioxide at 35 C and 15 MPa. The degree of crystallinity of functionalized PCL was shown to be influenced by the incorporated antibacterial agent. The presented results showed that the impregnated PCL samples are promising bactericidal compounds against L. innocua. Screening of antibacterial activity of functionalized PCL against a Methicillin-resistant Staphylococcus aureus (MRSA) strain showed a higher activity when a low bacterial inoculum level (2 × 104 Colony-forming Units/mL [CFU/mL]) was assayed.", publisher = "Elsevier", journal = "Journal of Supercritical Fluids", title = "Development of polycaprolactone scaffold with antibacterial activity by an integrated supercritical extraction and impregnation process", pages = "53-42", volume = "78", doi = "10.1016/j.supflu.2013.03.017" }
Fanovich, M.A., Ivanović, J., Mišić, D., Alvarez, M.V., Jaeger, P., Žižović, I.,& Eggers, R.. (2013). Development of polycaprolactone scaffold with antibacterial activity by an integrated supercritical extraction and impregnation process. in Journal of Supercritical Fluids Elsevier., 78, 42-53. https://doi.org/10.1016/j.supflu.2013.03.017
Fanovich M, Ivanović J, Mišić D, Alvarez M, Jaeger P, Žižović I, Eggers R. Development of polycaprolactone scaffold with antibacterial activity by an integrated supercritical extraction and impregnation process. in Journal of Supercritical Fluids. 2013;78:42-53. doi:10.1016/j.supflu.2013.03.017 .
Fanovich, M.A., Ivanović, J., Mišić, D., Alvarez, M.V., Jaeger, P., Žižović, Irena, Eggers, Rudolf, "Development of polycaprolactone scaffold with antibacterial activity by an integrated supercritical extraction and impregnation process" in Journal of Supercritical Fluids, 78 (2013):42-53, https://doi.org/10.1016/j.supflu.2013.03.017 . .