TY  - JOUR
AU  - Radonjić, Mia
AU  - Petrović, Jelena
AU  - Milivojević, Milena
AU  - Stevanović, Milena
AU  - Stojkovska, Jasmina
AU  - Obradović, Bojana
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5261
AB  - A multidisciplinary approach based on experiments and mathematical modeling was used in biomimetic system development for three-dimensional (3D) cultures of cancer cells. Specifically, two cancer cell lines, human embryonic teratocarcinoma NT2/D1 and rat glioma C6, were immobilized in alginate microbeads and microfibers, respectively, and cultured under static and flow conditions in perfusion bioreactors. At the same time, chemical engineering methods were applied to explain the obtained results. The superficial medium velocity of 80 μm s-1 induced lower viability of NT2/D1 cells in superficial microbead zones, implying adverse effects of fluid shear stresses estimated as ∼67 mPa. On the contrary, similar velocity (100 μm s-1) enhanced the proliferation of C6 glioma cells within microfibers compared to static controls. An additional study of silver release from nanocomposite Ag/honey/alginate microfibers under perfusion indicated that the medium partially flows through the hydrogel (interstitial velocity of ∼10 nm s-1). Thus, a diffusion-advection-reaction model described the mass transport to immobilized cells within microfibers. Substances with diffusion coefficients of ∼10-9-10-11 m2 s-1 are sufficiently supplied by diffusion only, while those with significantly lower diffusivities (∼10-19 m2 s-1) require additional convective transport. The present study demonstrates the selection and contribution of chemical engineering methods in tumor model system development.
PB  - National Library of Serbia
T2  - Chemical Industry and Chemical Engineering Quarterly
T2  - Chemical Industry and Chemical Engineering Quarterly
T1  - Chemical engineering methods in analyses of 3D cancer cell cultures: Hydrodinamic and mass transport considerations
EP  - 223
IS  - 3
SP  - 211
VL  - 28
DO  - 10.2298/CICEQ210607033R
ER  - 

@article{
author = "Radonjić, Mia and Petrović, Jelena and Milivojević, Milena and Stevanović, Milena and Stojkovska, Jasmina and Obradović, Bojana",
year = "2022",
abstract = "A multidisciplinary approach based on experiments and mathematical modeling was used in biomimetic system development for three-dimensional (3D) cultures of cancer cells. Specifically, two cancer cell lines, human embryonic teratocarcinoma NT2/D1 and rat glioma C6, were immobilized in alginate microbeads and microfibers, respectively, and cultured under static and flow conditions in perfusion bioreactors. At the same time, chemical engineering methods were applied to explain the obtained results. The superficial medium velocity of 80 μm s-1 induced lower viability of NT2/D1 cells in superficial microbead zones, implying adverse effects of fluid shear stresses estimated as ∼67 mPa. On the contrary, similar velocity (100 μm s-1) enhanced the proliferation of C6 glioma cells within microfibers compared to static controls. An additional study of silver release from nanocomposite Ag/honey/alginate microfibers under perfusion indicated that the medium partially flows through the hydrogel (interstitial velocity of ∼10 nm s-1). Thus, a diffusion-advection-reaction model described the mass transport to immobilized cells within microfibers. Substances with diffusion coefficients of ∼10-9-10-11 m2 s-1 are sufficiently supplied by diffusion only, while those with significantly lower diffusivities (∼10-19 m2 s-1) require additional convective transport. The present study demonstrates the selection and contribution of chemical engineering methods in tumor model system development.",
publisher = "National Library of Serbia",
journal = "Chemical Industry and Chemical Engineering Quarterly, Chemical Industry and Chemical Engineering Quarterly",
title = "Chemical engineering methods in analyses of 3D cancer cell cultures: Hydrodinamic and mass transport considerations",
pages = "223-211",
number = "3",
volume = "28",
doi = "10.2298/CICEQ210607033R"
}

Radonjić, M., Petrović, J., Milivojević, M., Stevanović, M., Stojkovska, J.,& Obradović, B.. (2022). Chemical engineering methods in analyses of 3D cancer cell cultures: Hydrodinamic and mass transport considerations. in Chemical Industry and Chemical Engineering Quarterly
National Library of Serbia., 28(3), 211-223.
https://doi.org/10.2298/CICEQ210607033R

Radonjić M, Petrović J, Milivojević M, Stevanović M, Stojkovska J, Obradović B. Chemical engineering methods in analyses of 3D cancer cell cultures: Hydrodinamic and mass transport considerations. in Chemical Industry and Chemical Engineering Quarterly. 2022;28(3):211-223.
doi:10.2298/CICEQ210607033R .

Radonjić, Mia, Petrović, Jelena, Milivojević, Milena, Stevanović, Milena, Stojkovska, Jasmina, Obradović, Bojana, "Chemical engineering methods in analyses of 3D cancer cell cultures: Hydrodinamic and mass transport considerations" in Chemical Industry and Chemical Engineering Quarterly, 28, no. 3 (2022):211-223,
https://doi.org/10.2298/CICEQ210607033R . .

TY  - CONF
AU  - Milošević, Mia
AU  - Banićević, Ivana
AU  - Pavlović, Marija
AU  - Milivojević, Milena
AU  - Stevanović, Milena
AU  - Stojkovska, Jasmina
AU  - Obradović, Bojana
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6351
PB  - Institute of Technical Sciences of SASA
C3  - Twentieth Young Researchers’ Conference - Materials Science and Engineering: Programme and the Book of Abstracts
T1  - Development of a physiologically relevant osteosarcoma model based on alginate scaffolds and perfusion bioreactor
SP  - 8
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6351
ER  - 

@conference{
author = "Milošević, Mia and Banićević, Ivana and Pavlović, Marija and Milivojević, Milena and Stevanović, Milena and Stojkovska, Jasmina and Obradović, Bojana",
year = "2022",
publisher = "Institute of Technical Sciences of SASA",
journal = "Twentieth Young Researchers’ Conference - Materials Science and Engineering: Programme and the Book of Abstracts",
title = "Development of a physiologically relevant osteosarcoma model based on alginate scaffolds and perfusion bioreactor",
pages = "8",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6351"
}

Milošević, M., Banićević, I., Pavlović, M., Milivojević, M., Stevanović, M., Stojkovska, J.,& Obradović, B.. (2022). Development of a physiologically relevant osteosarcoma model based on alginate scaffolds and perfusion bioreactor. in Twentieth Young Researchers’ Conference - Materials Science and Engineering: Programme and the Book of Abstracts
Institute of Technical Sciences of SASA., 8.
https://hdl.handle.net/21.15107/rcub_technorep_6351

Milošević M, Banićević I, Pavlović M, Milivojević M, Stevanović M, Stojkovska J, Obradović B. Development of a physiologically relevant osteosarcoma model based on alginate scaffolds and perfusion bioreactor. in Twentieth Young Researchers’ Conference - Materials Science and Engineering: Programme and the Book of Abstracts. 2022;:8.
https://hdl.handle.net/21.15107/rcub_technorep_6351 .

Milošević, Mia, Banićević, Ivana, Pavlović, Marija, Milivojević, Milena, Stevanović, Milena, Stojkovska, Jasmina, Obradović, Bojana, "Development of a physiologically relevant osteosarcoma model based on alginate scaffolds and perfusion bioreactor" in Twentieth Young Researchers’ Conference - Materials Science and Engineering: Programme and the Book of Abstracts (2022):8,
https://hdl.handle.net/21.15107/rcub_technorep_6351 .

TY  - CONF
AU  - Banićević, Ivana
AU  - Menshikh, Ksenia
AU  - Radonjić, Mia
AU  - Petrović, Jelena
AU  - Janković, Radmila
AU  - Milivojević, Milena
AU  - Stevanović, Milena
AU  - Stojkovska, Jasmina
AU  - Obradović, Bojana
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6350
PB  - Institute of Technical Sciences of SASA
C3  - Twentieth Young Researchers’ Conference - Materials Science and Engineering: Programme and the Book of Abstracts
T1  - Cellular self-assembly in a 3D osteosarcoma culture model based on alginate scaffolds and perfusion bioreactor
SP  - 9
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6350
ER  - 

@conference{
author = "Banićević, Ivana and Menshikh, Ksenia and Radonjić, Mia and Petrović, Jelena and Janković, Radmila and Milivojević, Milena and Stevanović, Milena and Stojkovska, Jasmina and Obradović, Bojana",
year = "2022",
publisher = "Institute of Technical Sciences of SASA",
journal = "Twentieth Young Researchers’ Conference - Materials Science and Engineering: Programme and the Book of Abstracts",
title = "Cellular self-assembly in a 3D osteosarcoma culture model based on alginate scaffolds and perfusion bioreactor",
pages = "9",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6350"
}

Banićević, I., Menshikh, K., Radonjić, M., Petrović, J., Janković, R., Milivojević, M., Stevanović, M., Stojkovska, J.,& Obradović, B.. (2022). Cellular self-assembly in a 3D osteosarcoma culture model based on alginate scaffolds and perfusion bioreactor. in Twentieth Young Researchers’ Conference - Materials Science and Engineering: Programme and the Book of Abstracts
Institute of Technical Sciences of SASA., 9.
https://hdl.handle.net/21.15107/rcub_technorep_6350

Banićević I, Menshikh K, Radonjić M, Petrović J, Janković R, Milivojević M, Stevanović M, Stojkovska J, Obradović B. Cellular self-assembly in a 3D osteosarcoma culture model based on alginate scaffolds and perfusion bioreactor. in Twentieth Young Researchers’ Conference - Materials Science and Engineering: Programme and the Book of Abstracts. 2022;:9.
https://hdl.handle.net/21.15107/rcub_technorep_6350 .

Banićević, Ivana, Menshikh, Ksenia, Radonjić, Mia, Petrović, Jelena, Janković, Radmila, Milivojević, Milena, Stevanović, Milena, Stojkovska, Jasmina, Obradović, Bojana, "Cellular self-assembly in a 3D osteosarcoma culture model based on alginate scaffolds and perfusion bioreactor" in Twentieth Young Researchers’ Conference - Materials Science and Engineering: Programme and the Book of Abstracts (2022):9,
https://hdl.handle.net/21.15107/rcub_technorep_6350 .

TY  - CONF
AU  - Menshikh, Ksenia
AU  - Banićević, Ivana
AU  - Radonjić, Mia
AU  - Miola, Marta
AU  - Stojkovska, Jasmina
AU  - Cochis, Andrea
AU  - Obradović, Bojana
AU  - Rimondini, Lia
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6216
C3  - Twentieth Young Researchers’ Conference - Materials Science and Engineering: Programme and the Book of Abstracts
T1  - Osteosarcoma in vitro: a step-by-step approach
SP  - 2-3
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6216
ER  - 

@conference{
author = "Menshikh, Ksenia and Banićević, Ivana and Radonjić, Mia and Miola, Marta and Stojkovska, Jasmina and Cochis, Andrea and Obradović, Bojana and Rimondini, Lia",
year = "2022",
journal = "Twentieth Young Researchers’ Conference - Materials Science and Engineering: Programme and the Book of Abstracts",
title = "Osteosarcoma in vitro: a step-by-step approach",
pages = "2-3",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6216"
}

Menshikh, K., Banićević, I., Radonjić, M., Miola, M., Stojkovska, J., Cochis, A., Obradović, B.,& Rimondini, L.. (2022). Osteosarcoma in vitro: a step-by-step approach. in Twentieth Young Researchers’ Conference - Materials Science and Engineering: Programme and the Book of Abstracts, 2-3.
https://hdl.handle.net/21.15107/rcub_technorep_6216

Menshikh K, Banićević I, Radonjić M, Miola M, Stojkovska J, Cochis A, Obradović B, Rimondini L. Osteosarcoma in vitro: a step-by-step approach. in Twentieth Young Researchers’ Conference - Materials Science and Engineering: Programme and the Book of Abstracts. 2022;:2-3.
https://hdl.handle.net/21.15107/rcub_technorep_6216 .

Menshikh, Ksenia, Banićević, Ivana, Radonjić, Mia, Miola, Marta, Stojkovska, Jasmina, Cochis, Andrea, Obradović, Bojana, Rimondini, Lia, "Osteosarcoma in vitro: a step-by-step approach" in Twentieth Young Researchers’ Conference - Materials Science and Engineering: Programme and the Book of Abstracts (2022):2-3,
https://hdl.handle.net/21.15107/rcub_technorep_6216 .

TY  - CONF
AU  - Banićević, Ivana
AU  - Radonjić, Mia
AU  - Pavlović, Marija
AU  - Milivojević, Milena
AU  - Stevanović, Milena
AU  - Stojkovska, Jasmina
AU  - Obradović, Bojana
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5028
AB  - Osteosarcoma is the most common type of bone cancer, which affects both children and
adults. Treatment of osteosarcoma exhibits slow progress due to inadequacy of both in vivo
animal models and 2D in vitro models regularly used for antitumor drug testing. Our
approach is to create a physiologically relevant 3D in vitro model for osteosarcoma cell
cultivation, which has the potential to overcome inherent weaknesses of 2D in vitro and
animal models. In order to imitate native osteosarcoma microenvironment, macroporous
alginate scaffolds with incorporated hydroxyapatite/β-tricalcium phosphate (HAp/β-TCP)
powder were produced with two compositions: 1 wt% alginate, 1 wt% powder and 2 wt.%
alginate, 2 wt% powder. Bioactivity and stability of the scaffolds were investigated under
biomimetic conditions of continuous flow of the culture medium in perfusion bioreactor at
the superficial medium velocity of 400 µm/s, which was reported in literature to be
beneficial for osteogenesis. Scaffolds with the higher alginate concentration was shown to be
more stable in the culture medium, since the scaffolds with the lower alginate concentration
disintegrated after 5-7 days under flow conditions. Biocompatibility of the obtained scaffolds
was investigated in short-term cultivation studies of murine osteosarcoma cells K7M2-wt
seeded onto the scaffolds. The scaffolds were cultivated in perfusion bioreactors at the
superficial flow velocity of 15 µm/s, while static cultures served as a control. After
cultivation, osteosarcoma cells remained adhered to the scaffold surface, expressed
metabolic activity and retained their initial proliferation ability while the flow was shown to
positively affect the cultivated cells.
PB  - Institute of Technical Sciences of SASA
C3  - Nineteenth Young Researchers’ Conference - Materials Science and Engineering: Program and the Book of Abstracts
T1  - Development of a physiologically relevant 3D in vitro model for osteosarcoma cell cultivation comprising alginate composite scaffolds and a perfusion bioreactor system
SP  - 6
VL  - 17
UR  - https://hdl.handle.net/21.15107/rcub_technorep_5028
ER  - 

@conference{
author = "Banićević, Ivana and Radonjić, Mia and Pavlović, Marija and Milivojević, Milena and Stevanović, Milena and Stojkovska, Jasmina and Obradović, Bojana",
year = "2021",
abstract = "Osteosarcoma is the most common type of bone cancer, which affects both children and
adults. Treatment of osteosarcoma exhibits slow progress due to inadequacy of both in vivo
animal models and 2D in vitro models regularly used for antitumor drug testing. Our
approach is to create a physiologically relevant 3D in vitro model for osteosarcoma cell
cultivation, which has the potential to overcome inherent weaknesses of 2D in vitro and
animal models. In order to imitate native osteosarcoma microenvironment, macroporous
alginate scaffolds with incorporated hydroxyapatite/β-tricalcium phosphate (HAp/β-TCP)
powder were produced with two compositions: 1 wt% alginate, 1 wt% powder and 2 wt.%
alginate, 2 wt% powder. Bioactivity and stability of the scaffolds were investigated under
biomimetic conditions of continuous flow of the culture medium in perfusion bioreactor at
the superficial medium velocity of 400 µm/s, which was reported in literature to be
beneficial for osteogenesis. Scaffolds with the higher alginate concentration was shown to be
more stable in the culture medium, since the scaffolds with the lower alginate concentration
disintegrated after 5-7 days under flow conditions. Biocompatibility of the obtained scaffolds
was investigated in short-term cultivation studies of murine osteosarcoma cells K7M2-wt
seeded onto the scaffolds. The scaffolds were cultivated in perfusion bioreactors at the
superficial flow velocity of 15 µm/s, while static cultures served as a control. After
cultivation, osteosarcoma cells remained adhered to the scaffold surface, expressed
metabolic activity and retained their initial proliferation ability while the flow was shown to
positively affect the cultivated cells.",
publisher = "Institute of Technical Sciences of SASA",
journal = "Nineteenth Young Researchers’ Conference - Materials Science and Engineering: Program and the Book of Abstracts",
title = "Development of a physiologically relevant 3D in vitro model for osteosarcoma cell cultivation comprising alginate composite scaffolds and a perfusion bioreactor system",
pages = "6",
volume = "17",
url = "https://hdl.handle.net/21.15107/rcub_technorep_5028"
}

Banićević, I., Radonjić, M., Pavlović, M., Milivojević, M., Stevanović, M., Stojkovska, J.,& Obradović, B.. (2021). Development of a physiologically relevant 3D in vitro model for osteosarcoma cell cultivation comprising alginate composite scaffolds and a perfusion bioreactor system. in Nineteenth Young Researchers’ Conference - Materials Science and Engineering: Program and the Book of Abstracts
Institute of Technical Sciences of SASA., 17, 6.
https://hdl.handle.net/21.15107/rcub_technorep_5028

Banićević I, Radonjić M, Pavlović M, Milivojević M, Stevanović M, Stojkovska J, Obradović B. Development of a physiologically relevant 3D in vitro model for osteosarcoma cell cultivation comprising alginate composite scaffolds and a perfusion bioreactor system. in Nineteenth Young Researchers’ Conference - Materials Science and Engineering: Program and the Book of Abstracts. 2021;17:6.
https://hdl.handle.net/21.15107/rcub_technorep_5028 .

Banićević, Ivana, Radonjić, Mia, Pavlović, Marija, Milivojević, Milena, Stevanović, Milena, Stojkovska, Jasmina, Obradović, Bojana, "Development of a physiologically relevant 3D in vitro model for osteosarcoma cell cultivation comprising alginate composite scaffolds and a perfusion bioreactor system" in Nineteenth Young Researchers’ Conference - Materials Science and Engineering: Program and the Book of Abstracts, 17 (2021):6,
https://hdl.handle.net/21.15107/rcub_technorep_5028 .

TY  - CONF
AU  - Petrović, Jelena
AU  - Radonjić, Mia
AU  - Stojkovska, Jasmina
AU  - Obradović, Bojana
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5033
AB  - Glioblastoma is the most common and aggressive malignant brain tumor in adults. Existing
treatment choices that include surgery, radiation and chemotherapy are not successful in
long-term survival, while development of new anticancer drugs is being held back by the
lack of adequate model systems for anticancer drug testing. Namely, in traditionally used
two-dimensional (2D) monolayer cancer cell cultures the native cell morphology, polarity
and interactions between both cells and cells and extracellular components are either
changed or absent, while studies on animals often produce misleading results due to
interspecies differences. Hence, there is a pressing need for new glioblastoma model systems
that provide more in vivo-like environment for investigation and development of new
anticancer drugs. The aim of this work was to develop a biomimetic 3D environment for
cultivation of glioblastoma cells based on alginate microfibers as cell carriers and perfusion
bioreactors. Previous studies have shown that static cultures of cervical cancer cells SiHa
immobilized in alginate microfibers may be diffusion limited while perfusion, which
enhanced mass transport, has induced negative effects on human embryonic teratocarcinoma
cells NTERA-2 in superficial zones of alginate microbeads by hydrodynamic shear stresses.
Thus, in the present study, the specific focus was on optimization of cell concentration
within microfibers and regimes of cultivation to achieve beneficial effects of fluid flow in
perfusion bioreactors. A series of experiments were conducted in which the concentration of
rat glioma cells C6 was varied between 2 and 8 × 10
6
cell cm
-3
at several flowrates and
regimens of static and perfusion culture periods. Mixed results were obtained implying that
efficient mass transport has a higher effect in microfiber cultures at lower cell concentrations
(i.e. ~2 × 10
6
cell cm
-3
). In specific, medium flow at the superficial velocity of 100 µm s
-1
induced considerable cell proliferation as compared to control static cultures, which
maintained the initial cell numbers. Mathematical modelling indicated that the convective
transport of substances with low diffusion coefficients (~10
-19 m
2
s
-1
) may have induced the
observed positive effects. Still, exact relations of cultivation conditions and cell responses in
terms of viability, proliferation and metabolic activity should be further investigated.
PB  - Institute of Technical Sciences of SASA
C3  - Nineteenth Young Researchers’ Conference - Materials Science and Engineering: Program and the Book of Abstracts
T1  - Optimization of Bioreactor Cultures of Glioblastoma Cells Immobilized in Alginate Microfibers
SP  - 8
VL  - 18
UR  - https://hdl.handle.net/21.15107/rcub_technorep_5033
ER  - 

@conference{
author = "Petrović, Jelena and Radonjić, Mia and Stojkovska, Jasmina and Obradović, Bojana",
year = "2021",
abstract = "Glioblastoma is the most common and aggressive malignant brain tumor in adults. Existing
treatment choices that include surgery, radiation and chemotherapy are not successful in
long-term survival, while development of new anticancer drugs is being held back by the
lack of adequate model systems for anticancer drug testing. Namely, in traditionally used
two-dimensional (2D) monolayer cancer cell cultures the native cell morphology, polarity
and interactions between both cells and cells and extracellular components are either
changed or absent, while studies on animals often produce misleading results due to
interspecies differences. Hence, there is a pressing need for new glioblastoma model systems
that provide more in vivo-like environment for investigation and development of new
anticancer drugs. The aim of this work was to develop a biomimetic 3D environment for
cultivation of glioblastoma cells based on alginate microfibers as cell carriers and perfusion
bioreactors. Previous studies have shown that static cultures of cervical cancer cells SiHa
immobilized in alginate microfibers may be diffusion limited while perfusion, which
enhanced mass transport, has induced negative effects on human embryonic teratocarcinoma
cells NTERA-2 in superficial zones of alginate microbeads by hydrodynamic shear stresses.
Thus, in the present study, the specific focus was on optimization of cell concentration
within microfibers and regimes of cultivation to achieve beneficial effects of fluid flow in
perfusion bioreactors. A series of experiments were conducted in which the concentration of
rat glioma cells C6 was varied between 2 and 8 × 10
6
cell cm
-3
at several flowrates and
regimens of static and perfusion culture periods. Mixed results were obtained implying that
efficient mass transport has a higher effect in microfiber cultures at lower cell concentrations
(i.e. ~2 × 10
6
cell cm
-3
). In specific, medium flow at the superficial velocity of 100 µm s
-1
induced considerable cell proliferation as compared to control static cultures, which
maintained the initial cell numbers. Mathematical modelling indicated that the convective
transport of substances with low diffusion coefficients (~10
-19 m
2
s
-1
) may have induced the
observed positive effects. Still, exact relations of cultivation conditions and cell responses in
terms of viability, proliferation and metabolic activity should be further investigated.",
publisher = "Institute of Technical Sciences of SASA",
journal = "Nineteenth Young Researchers’ Conference - Materials Science and Engineering: Program and the Book of Abstracts",
title = "Optimization of Bioreactor Cultures of Glioblastoma Cells Immobilized in Alginate Microfibers",
pages = "8",
volume = "18",
url = "https://hdl.handle.net/21.15107/rcub_technorep_5033"
}

Petrović, J., Radonjić, M., Stojkovska, J.,& Obradović, B.. (2021). Optimization of Bioreactor Cultures of Glioblastoma Cells Immobilized in Alginate Microfibers. in Nineteenth Young Researchers’ Conference - Materials Science and Engineering: Program and the Book of Abstracts
Institute of Technical Sciences of SASA., 18, 8.
https://hdl.handle.net/21.15107/rcub_technorep_5033

Petrović J, Radonjić M, Stojkovska J, Obradović B. Optimization of Bioreactor Cultures of Glioblastoma Cells Immobilized in Alginate Microfibers. in Nineteenth Young Researchers’ Conference - Materials Science and Engineering: Program and the Book of Abstracts. 2021;18:8.
https://hdl.handle.net/21.15107/rcub_technorep_5033 .

Petrović, Jelena, Radonjić, Mia, Stojkovska, Jasmina, Obradović, Bojana, "Optimization of Bioreactor Cultures of Glioblastoma Cells Immobilized in Alginate Microfibers" in Nineteenth Young Researchers’ Conference - Materials Science and Engineering: Program and the Book of Abstracts, 18 (2021):8,
https://hdl.handle.net/21.15107/rcub_technorep_5033 .

TY  - CONF
AU  - Petrović, Jelena
AU  - Radonjić, Mia
AU  - Stojkovska, Jasmina
AU  - Obradović, Bojana
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6211
AB  - Cancer is the second leading cause of death globally, making the search for its cure one of the most important challenges of the 21st century. With ethical questions regarding animal testing and inconsistency of results of cancer drug testing in standard two-dimensional (2D) monolayer cell cultures with the results in vivo, there is a pressing need for better in vitro models of human cancers that will provide more relevant systems for cancer drug screening. Three-dimensional (3D) in vitro systems based on natural polymers with immobilized cancer cells that mimic cancerous tissue and bioreactors that provide relevant chemical and physical signals could close the gap between 2D in vitro and in vivo cancer models. The aim of this study was to optimize culture conditions for the rat glioma cell line C6 immobilized in alginate microfibers in perfusion bioreactors in terms of cell density and perfusion rate. In this study we investigated following sets of parameters: perfusion rate of 0.12, 0.25 and 0.30 ml min-1 coupled with the cell density of 4∙106 cells ml-1 , and perfusion rate of 0.30 ml min-1 coupled with the cell density of 8∙106 cells ml-1 . Microfiber cultures under static conditions in Petri dishes served as controls. The results have shown that the perfusion rate of 0.30 ml min-1 in combination with the cell density of 8∙106 cells ml-1 yields higher cell viability and proliferation compared to the control static culture. These results indicate the importance of culture medium perfusion in the bioreactor for improved mass transfer of nutrients and oxygen to alginate microfibers so that the investigated system shows potentials for use as a model system in cancer research.
PB  - Materials Research Society of Serbia
C3  - 22nd Annual Conference YUCOMAT 2021, Herceg Novi, Montenegro
T1  - Optimization of in vitro conditions for 3D culture of rat glioma cells
SP  - 80
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6211
ER  - 

@conference{
author = "Petrović, Jelena and Radonjić, Mia and Stojkovska, Jasmina and Obradović, Bojana",
year = "2021",
abstract = "Cancer is the second leading cause of death globally, making the search for its cure one of the most important challenges of the 21st century. With ethical questions regarding animal testing and inconsistency of results of cancer drug testing in standard two-dimensional (2D) monolayer cell cultures with the results in vivo, there is a pressing need for better in vitro models of human cancers that will provide more relevant systems for cancer drug screening. Three-dimensional (3D) in vitro systems based on natural polymers with immobilized cancer cells that mimic cancerous tissue and bioreactors that provide relevant chemical and physical signals could close the gap between 2D in vitro and in vivo cancer models. The aim of this study was to optimize culture conditions for the rat glioma cell line C6 immobilized in alginate microfibers in perfusion bioreactors in terms of cell density and perfusion rate. In this study we investigated following sets of parameters: perfusion rate of 0.12, 0.25 and 0.30 ml min-1 coupled with the cell density of 4∙106 cells ml-1 , and perfusion rate of 0.30 ml min-1 coupled with the cell density of 8∙106 cells ml-1 . Microfiber cultures under static conditions in Petri dishes served as controls. The results have shown that the perfusion rate of 0.30 ml min-1 in combination with the cell density of 8∙106 cells ml-1 yields higher cell viability and proliferation compared to the control static culture. These results indicate the importance of culture medium perfusion in the bioreactor for improved mass transfer of nutrients and oxygen to alginate microfibers so that the investigated system shows potentials for use as a model system in cancer research.",
publisher = "Materials Research Society of Serbia",
journal = "22nd Annual Conference YUCOMAT 2021, Herceg Novi, Montenegro",
title = "Optimization of in vitro conditions for 3D culture of rat glioma cells",
pages = "80",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6211"
}

Petrović, J., Radonjić, M., Stojkovska, J.,& Obradović, B.. (2021). Optimization of in vitro conditions for 3D culture of rat glioma cells. in 22nd Annual Conference YUCOMAT 2021, Herceg Novi, Montenegro
Materials Research Society of Serbia., 80.
https://hdl.handle.net/21.15107/rcub_technorep_6211

Petrović J, Radonjić M, Stojkovska J, Obradović B. Optimization of in vitro conditions for 3D culture of rat glioma cells. in 22nd Annual Conference YUCOMAT 2021, Herceg Novi, Montenegro. 2021;:80.
https://hdl.handle.net/21.15107/rcub_technorep_6211 .

Petrović, Jelena, Radonjić, Mia, Stojkovska, Jasmina, Obradović, Bojana, "Optimization of in vitro conditions for 3D culture of rat glioma cells" in 22nd Annual Conference YUCOMAT 2021, Herceg Novi, Montenegro (2021):80,
https://hdl.handle.net/21.15107/rcub_technorep_6211 .

TY  - CONF
AU  - Radonjić, Mia
AU  - Petrović, Jelena
AU  - Milivojević, Milena
AU  - Stevanović, Milena
AU  - Stojkovska, Jasmina
AU  - Obradović, Bojana
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6354
AB  - Cancer cell immobilization in polymer hydrogels serving as extracellular matrices and cultivation in perfusion bioreactors
that provide appropriate chemical signals, efficient mass transfer and hydrodynamic shear stresses is a promising strategy
for development of physiologically relevant tumor models. In this work, perfusion cultures of 2 cancer cell types (C6 rat
glioma and embryonal carcinoma NT2/D1 cells) immobilized in alginate microgels were established, while static cultures
served as controls. Continuous perfusion had different effects on the cultured cells inducing enhanced proliferation of the
glioma cells immobilized in microfibers (8x10^6 cell/ml), while reducing the viability of the NT2/D1 cells immobilized in
microbeads (1x10^6 cell/ml). In order to elucidate the observed effects, chemical engineering principles were applied to
assess mass transfer and hydrodynamic conditions. The second Fick’s law was solved analytically while the diffusionadvection-reaction equation was solved numerically to model mass transport in the static and bioreactor cultures,
respectively. Moreover, Reynolds numbers, pressure drops and shear stresses in bioreactor cultures were calculated for
assessment of flow regime and hydrodynamic conditions. The modeling results have indicated that oxygen transport is
diffusion-controlled through the alginate hydrogel, while medium perfusion improves mass transfer of larger compounds
having smaller diffusion coefficients (∼10^(-13) m^2/s), which possibly stimulated glioma cell proliferation. On the other
hand, the obtained shear stress (~50 mPa) in the perfused packed bed of microbeads was above physiological levels, which
provided the explanation of the poor NT2/D1 cell survival. This study stresses the importance of multidisciplinary approach
in addressing such multifactorial diseases as cancer.
C3  - 6th World TERMIS Conference 2021 Abstract Book
T1  - Optimization of 3D cancer cell culture conditions by application of chemical engineering principles
SP  - 1408
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6354
ER  - 

@conference{
author = "Radonjić, Mia and Petrović, Jelena and Milivojević, Milena and Stevanović, Milena and Stojkovska, Jasmina and Obradović, Bojana",
year = "2021",
abstract = "Cancer cell immobilization in polymer hydrogels serving as extracellular matrices and cultivation in perfusion bioreactors
that provide appropriate chemical signals, efficient mass transfer and hydrodynamic shear stresses is a promising strategy
for development of physiologically relevant tumor models. In this work, perfusion cultures of 2 cancer cell types (C6 rat
glioma and embryonal carcinoma NT2/D1 cells) immobilized in alginate microgels were established, while static cultures
served as controls. Continuous perfusion had different effects on the cultured cells inducing enhanced proliferation of the
glioma cells immobilized in microfibers (8x10^6 cell/ml), while reducing the viability of the NT2/D1 cells immobilized in
microbeads (1x10^6 cell/ml). In order to elucidate the observed effects, chemical engineering principles were applied to
assess mass transfer and hydrodynamic conditions. The second Fick’s law was solved analytically while the diffusionadvection-reaction equation was solved numerically to model mass transport in the static and bioreactor cultures,
respectively. Moreover, Reynolds numbers, pressure drops and shear stresses in bioreactor cultures were calculated for
assessment of flow regime and hydrodynamic conditions. The modeling results have indicated that oxygen transport is
diffusion-controlled through the alginate hydrogel, while medium perfusion improves mass transfer of larger compounds
having smaller diffusion coefficients (∼10^(-13) m^2/s), which possibly stimulated glioma cell proliferation. On the other
hand, the obtained shear stress (~50 mPa) in the perfused packed bed of microbeads was above physiological levels, which
provided the explanation of the poor NT2/D1 cell survival. This study stresses the importance of multidisciplinary approach
in addressing such multifactorial diseases as cancer.",
journal = "6th World TERMIS Conference 2021 Abstract Book",
title = "Optimization of 3D cancer cell culture conditions by application of chemical engineering principles",
pages = "1408",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6354"
}

Radonjić, M., Petrović, J., Milivojević, M., Stevanović, M., Stojkovska, J.,& Obradović, B.. (2021). Optimization of 3D cancer cell culture conditions by application of chemical engineering principles. in 6th World TERMIS Conference 2021 Abstract Book, 1408.
https://hdl.handle.net/21.15107/rcub_technorep_6354

Radonjić M, Petrović J, Milivojević M, Stevanović M, Stojkovska J, Obradović B. Optimization of 3D cancer cell culture conditions by application of chemical engineering principles. in 6th World TERMIS Conference 2021 Abstract Book. 2021;:1408.
https://hdl.handle.net/21.15107/rcub_technorep_6354 .

Radonjić, Mia, Petrović, Jelena, Milivojević, Milena, Stevanović, Milena, Stojkovska, Jasmina, Obradović, Bojana, "Optimization of 3D cancer cell culture conditions by application of chemical engineering principles" in 6th World TERMIS Conference 2021 Abstract Book (2021):1408,
https://hdl.handle.net/21.15107/rcub_technorep_6354 .

TY  - CONF
AU  - Stojkovska, Jasmina
AU  - Stanković, Tijana
AU  - Dragoj, Miodrag
AU  - Petrović, Jelena
AU  - Radonjić, Mia
AU  - Pešić, Milica
AU  - Obradović, Bojana
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6353
AB  - The aim of this work was to develop a 3D microenvironment for glioblastoma brain tumor engineering based on alginate
hydrogels as a matrix for cell immobilization followed by cultivation in a biomimetic perfusion bioreactor. Alginate
microfibers with immobilized cells were obtained by a simple extrusion technique. We have examined the influence of the
needle diameter (22G - 28G), cell density in alginate solution (1 x 106 - 8 x 106 cells/ml) and different cancer cell lines (rat
C6 and human U251 and U87) on cell immobilization efficiency and viability. The best alginate microfibers (500 µm in
diameter) with all immobilized cells were obtained by applying a 25G needle with a minimal cell density of 4 x 106 cells/ml.
The obtained microfibers with immobilized cells (C6 and U87) were cultivated in a perfusion bioreactor at the continuous
medium flowrate in the range 0.05-0.30 ml/min over short- and long-term cultivation periods. The results have shown that
the flowrate of 0.30 ml/min, corresponding to the superficial velocity of 100 µm/s, in combination with the C6 cell density
of 8 x 106 cells/ml in short-term studies yielded higher cell viabilities and proliferation as compared to the control static
culture. In addition, U87 cells immobilized in alginate microfibers at the density of 4 x 106 cells/ml after long-term
cultivation at the medium flowrate of 0.05 ml/min (superficial velocity of 15 µm/s) stayed viable.
The overall results have shown potentials of the applied approach for tumor engineering provided optimization of
cultivation conditions for each cell type.
C3  - 6th World TERMIS Conference 2021 Abstract Book
T1  - Development of 3D microenvironment for engineering of glioblastoma brain tumor
SP  - 930
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6353
ER  - 

@conference{
author = "Stojkovska, Jasmina and Stanković, Tijana and Dragoj, Miodrag and Petrović, Jelena and Radonjić, Mia and Pešić, Milica and Obradović, Bojana",
year = "2021",
abstract = "The aim of this work was to develop a 3D microenvironment for glioblastoma brain tumor engineering based on alginate
hydrogels as a matrix for cell immobilization followed by cultivation in a biomimetic perfusion bioreactor. Alginate
microfibers with immobilized cells were obtained by a simple extrusion technique. We have examined the influence of the
needle diameter (22G - 28G), cell density in alginate solution (1 x 106 - 8 x 106 cells/ml) and different cancer cell lines (rat
C6 and human U251 and U87) on cell immobilization efficiency and viability. The best alginate microfibers (500 µm in
diameter) with all immobilized cells were obtained by applying a 25G needle with a minimal cell density of 4 x 106 cells/ml.
The obtained microfibers with immobilized cells (C6 and U87) were cultivated in a perfusion bioreactor at the continuous
medium flowrate in the range 0.05-0.30 ml/min over short- and long-term cultivation periods. The results have shown that
the flowrate of 0.30 ml/min, corresponding to the superficial velocity of 100 µm/s, in combination with the C6 cell density
of 8 x 106 cells/ml in short-term studies yielded higher cell viabilities and proliferation as compared to the control static
culture. In addition, U87 cells immobilized in alginate microfibers at the density of 4 x 106 cells/ml after long-term
cultivation at the medium flowrate of 0.05 ml/min (superficial velocity of 15 µm/s) stayed viable.
The overall results have shown potentials of the applied approach for tumor engineering provided optimization of
cultivation conditions for each cell type.",
journal = "6th World TERMIS Conference 2021 Abstract Book",
title = "Development of 3D microenvironment for engineering of glioblastoma brain tumor",
pages = "930",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6353"
}

Stojkovska, J., Stanković, T., Dragoj, M., Petrović, J., Radonjić, M., Pešić, M.,& Obradović, B.. (2021). Development of 3D microenvironment for engineering of glioblastoma brain tumor. in 6th World TERMIS Conference 2021 Abstract Book, 930.
https://hdl.handle.net/21.15107/rcub_technorep_6353

Stojkovska J, Stanković T, Dragoj M, Petrović J, Radonjić M, Pešić M, Obradović B. Development of 3D microenvironment for engineering of glioblastoma brain tumor. in 6th World TERMIS Conference 2021 Abstract Book. 2021;:930.
https://hdl.handle.net/21.15107/rcub_technorep_6353 .

Stojkovska, Jasmina, Stanković, Tijana, Dragoj, Miodrag, Petrović, Jelena, Radonjić, Mia, Pešić, Milica, Obradović, Bojana, "Development of 3D microenvironment for engineering of glioblastoma brain tumor" in 6th World TERMIS Conference 2021 Abstract Book (2021):930,
https://hdl.handle.net/21.15107/rcub_technorep_6353 .

TY  - CONF
AU  - Zvicer, Jovana
AU  - Radonjić, Mia
AU  - Kovrlija, Ilijana
AU  - Medić, Ana
AU  - Novak, Saša
AU  - Obradović, Bojana
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6352
AB  - Bilayer scaffolds based on gellan gum (GG) and nanoparticulate bioactive-glass (BAG) were developed by an integrative
approach based on engineering principles and characterization in biomimetic bioreactors. The osteo-inductive GG-BAG
layer containing 2 % w/w GG and 2 % w/w BAG (composition: 70 n/n % SiO2, 30 n/n % CaO) was produced by gelation
followed by freeze-drying to obtain open porosity in axial and radial directions. The chondral layer was obtained by
dispensing a warm 2 % w/w GG solution at 60˚C over the frozen macroporous GG-BAG layer at -25˚C. The temperatures
were optimized by applying a one-dimensional unsteady-state heat transfer model so to obtain a thin integration zone, 0.5
– 1 mm thick. The scaffolds were evaluated regarding bioactivity in a biomimetic bioreactor with specially designed
chambers to provide supply of two media relevant for chondral and bone tissues. In the present experiment, simulated
body fluid (SBF) was supplied countercurrently continuously during 14 days of the experiment (1.1 ml min-1 flowrate), while
dynamic compression (5 % deformation, 0.68 Hz frequency, 337.5 µm s-1 loading rate, 1 h / day) was applied on the
chondral layer, from day 7 to day 14. SEM analyses have confirmed the retained integrity of the scaffolds, as well as
formation of hydroxyapatite (HAp) uniformly throughout the osteo-layer of the scaffolds.Significantly higher bioactivity
under biomimetic conditions compared to static controls resulted in slightly but significantly increased compression
modulus. These results indicated a high potential of the applied integrative strategy for the development of biomimetic
bilayer scaffolds.
C3  - 6th World TERMIS Conference 2021 Abstract Book
T1  - An integrative approach in developing scaffolds based on gellan gum and bioactive glass aimed for osteochondral tissue engineering
SP  - 663
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6352
ER  - 

@conference{
author = "Zvicer, Jovana and Radonjić, Mia and Kovrlija, Ilijana and Medić, Ana and Novak, Saša and Obradović, Bojana",
year = "2021",
abstract = "Bilayer scaffolds based on gellan gum (GG) and nanoparticulate bioactive-glass (BAG) were developed by an integrative
approach based on engineering principles and characterization in biomimetic bioreactors. The osteo-inductive GG-BAG
layer containing 2 % w/w GG and 2 % w/w BAG (composition: 70 n/n % SiO2, 30 n/n % CaO) was produced by gelation
followed by freeze-drying to obtain open porosity in axial and radial directions. The chondral layer was obtained by
dispensing a warm 2 % w/w GG solution at 60˚C over the frozen macroporous GG-BAG layer at -25˚C. The temperatures
were optimized by applying a one-dimensional unsteady-state heat transfer model so to obtain a thin integration zone, 0.5
– 1 mm thick. The scaffolds were evaluated regarding bioactivity in a biomimetic bioreactor with specially designed
chambers to provide supply of two media relevant for chondral and bone tissues. In the present experiment, simulated
body fluid (SBF) was supplied countercurrently continuously during 14 days of the experiment (1.1 ml min-1 flowrate), while
dynamic compression (5 % deformation, 0.68 Hz frequency, 337.5 µm s-1 loading rate, 1 h / day) was applied on the
chondral layer, from day 7 to day 14. SEM analyses have confirmed the retained integrity of the scaffolds, as well as
formation of hydroxyapatite (HAp) uniformly throughout the osteo-layer of the scaffolds.Significantly higher bioactivity
under biomimetic conditions compared to static controls resulted in slightly but significantly increased compression
modulus. These results indicated a high potential of the applied integrative strategy for the development of biomimetic
bilayer scaffolds.",
journal = "6th World TERMIS Conference 2021 Abstract Book",
title = "An integrative approach in developing scaffolds based on gellan gum and bioactive glass aimed for osteochondral tissue engineering",
pages = "663",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6352"
}

Zvicer, J., Radonjić, M., Kovrlija, I., Medić, A., Novak, S.,& Obradović, B.. (2021). An integrative approach in developing scaffolds based on gellan gum and bioactive glass aimed for osteochondral tissue engineering. in 6th World TERMIS Conference 2021 Abstract Book, 663.
https://hdl.handle.net/21.15107/rcub_technorep_6352

Zvicer J, Radonjić M, Kovrlija I, Medić A, Novak S, Obradović B. An integrative approach in developing scaffolds based on gellan gum and bioactive glass aimed for osteochondral tissue engineering. in 6th World TERMIS Conference 2021 Abstract Book. 2021;:663.
https://hdl.handle.net/21.15107/rcub_technorep_6352 .

Zvicer, Jovana, Radonjić, Mia, Kovrlija, Ilijana, Medić, Ana, Novak, Saša, Obradović, Bojana, "An integrative approach in developing scaffolds based on gellan gum and bioactive glass aimed for osteochondral tissue engineering" in 6th World TERMIS Conference 2021 Abstract Book (2021):663,
https://hdl.handle.net/21.15107/rcub_technorep_6352 .

TY  - CONF
AU  - Radonjić, Mia
AU  - Zvicer, Jovana
AU  - Obradović, Bojana
PY  - 2017
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6347
AB  - Scaffolds used for osteochondral tissue engineering should comprise two distinct regions: a
bottom region with characteristics corresponding to bone tissue, such as a porous structure
with mineral components (predominantly hydroxyapatite), and a top region with
characteristics of articular cartilage, which is gelatinous with high water content. In this
work, we have investigated possibilities to formulate and optimize a procedure for obtaining
such biphasic scaffolds based on gellan gum (GG). A porous base layer of the scaffold was
obtained by lyophilization of the 2 % GG hydrogel with dispersed bioactive glass
nanoparticles, as hydroxyapatite precursors. Next, different procedures were investigated to
produce the upper GG hydrogel such as partial immersion of the porous layer in the GG
solution and pouring the GG solution over the porous layer at different moisture conditions
and temperatures. A simple mathematical model was derived and subsequently
experimentally validated to find optimal temperatures of the porous layer, GG solution and
the surrounding environment to provide adequate gelation rate to form the GG hydrogel on
top of the porous layer with a thin interfacial zone.
PB  - Institute of Technical Sciences of SASA
C3  - Seventieth Young Researchers’ Conference - Materials Science and Engineering: Programme and the Book of Abstracts
T1  - Development and optimization of the production procedure of biphasic scaffolds for osteochondral tissue engineering
SP  - 4
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6347
ER  - 

@conference{
author = "Radonjić, Mia and Zvicer, Jovana and Obradović, Bojana",
year = "2017",
abstract = "Scaffolds used for osteochondral tissue engineering should comprise two distinct regions: a
bottom region with characteristics corresponding to bone tissue, such as a porous structure
with mineral components (predominantly hydroxyapatite), and a top region with
characteristics of articular cartilage, which is gelatinous with high water content. In this
work, we have investigated possibilities to formulate and optimize a procedure for obtaining
such biphasic scaffolds based on gellan gum (GG). A porous base layer of the scaffold was
obtained by lyophilization of the 2 % GG hydrogel with dispersed bioactive glass
nanoparticles, as hydroxyapatite precursors. Next, different procedures were investigated to
produce the upper GG hydrogel such as partial immersion of the porous layer in the GG
solution and pouring the GG solution over the porous layer at different moisture conditions
and temperatures. A simple mathematical model was derived and subsequently
experimentally validated to find optimal temperatures of the porous layer, GG solution and
the surrounding environment to provide adequate gelation rate to form the GG hydrogel on
top of the porous layer with a thin interfacial zone.",
publisher = "Institute of Technical Sciences of SASA",
journal = "Seventieth Young Researchers’ Conference - Materials Science and Engineering: Programme and the Book of Abstracts",
title = "Development and optimization of the production procedure of biphasic scaffolds for osteochondral tissue engineering",
pages = "4",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6347"
}

Radonjić, M., Zvicer, J.,& Obradović, B.. (2017). Development and optimization of the production procedure of biphasic scaffolds for osteochondral tissue engineering. in Seventieth Young Researchers’ Conference - Materials Science and Engineering: Programme and the Book of Abstracts
Institute of Technical Sciences of SASA., 4.
https://hdl.handle.net/21.15107/rcub_technorep_6347

Radonjić M, Zvicer J, Obradović B. Development and optimization of the production procedure of biphasic scaffolds for osteochondral tissue engineering. in Seventieth Young Researchers’ Conference - Materials Science and Engineering: Programme and the Book of Abstracts. 2017;:4.
https://hdl.handle.net/21.15107/rcub_technorep_6347 .

Radonjić, Mia, Zvicer, Jovana, Obradović, Bojana, "Development and optimization of the production procedure of biphasic scaffolds for osteochondral tissue engineering" in Seventieth Young Researchers’ Conference - Materials Science and Engineering: Programme and the Book of Abstracts (2017):4,
https://hdl.handle.net/21.15107/rcub_technorep_6347 .

TY  - JOUR
AU  - Sangsong, S.
AU  - Srimala, K.
AU  - Radonjić, Mia
AU  - Tungkamani, S.
AU  - Sornchamni, T.
AU  - Phongaksorn, M.
PY  - 2017
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6346
AB  - High temperature water gas shift (HT-WGS) is an important catalytic process connected with reforming process in hydrogen production. Ni/CeO2-Al2O3 (or Al2O3) catalysts were studied in this work on the effect of catalyst preparation method toward the physicochemical properties and the HT-WGS activity. Ni/CeO2-Al2O3 were prepared by sol-gel and impregnation methods whereas Ni/Al2O3 was prepared by impregnation method. The catalyst samples were characterized by XRD, H2-TPR and H2-TPD techniques. The catalytic activities of HT-WGS catalysts was demonstrated at 550°C, GHSV of 2x105 mLh-1gcat-1and steam-to-CO ratio of 3. Nickel was detected as a nickel aluminate phase in the calcined catalyst. Ni strongly interacted with support in the calcined catalyst prepared by sol-gel method. The strong metal-support interaction can be resisted by preparing catalyst via impregnation and CeO2 can promote the H2O dissociation in HT-WGS mechanism. The highest metal dispersion, largest metal surface area and greatest HT-WGS activity were consequently achieved by Ni/CeO2-Al2O3 prepared from impregnation method.
T2  - Key Engineering Materials
T1  - The Effect of Preparation Method on Ni/Ce/Al Catalyst for High Temperature Water-Gas Shift Reaction
EP  - 175
SP  - 171
VL  - 757
DO  - 10.4028/www.scientific.net/KEM.757.171
ER  - 

@article{
author = "Sangsong, S. and Srimala, K. and Radonjić, Mia and Tungkamani, S. and Sornchamni, T. and Phongaksorn, M.",
year = "2017",
abstract = "High temperature water gas shift (HT-WGS) is an important catalytic process connected with reforming process in hydrogen production. Ni/CeO2-Al2O3 (or Al2O3) catalysts were studied in this work on the effect of catalyst preparation method toward the physicochemical properties and the HT-WGS activity. Ni/CeO2-Al2O3 were prepared by sol-gel and impregnation methods whereas Ni/Al2O3 was prepared by impregnation method. The catalyst samples were characterized by XRD, H2-TPR and H2-TPD techniques. The catalytic activities of HT-WGS catalysts was demonstrated at 550°C, GHSV of 2x105 mLh-1gcat-1and steam-to-CO ratio of 3. Nickel was detected as a nickel aluminate phase in the calcined catalyst. Ni strongly interacted with support in the calcined catalyst prepared by sol-gel method. The strong metal-support interaction can be resisted by preparing catalyst via impregnation and CeO2 can promote the H2O dissociation in HT-WGS mechanism. The highest metal dispersion, largest metal surface area and greatest HT-WGS activity were consequently achieved by Ni/CeO2-Al2O3 prepared from impregnation method.",
journal = "Key Engineering Materials",
title = "The Effect of Preparation Method on Ni/Ce/Al Catalyst for High Temperature Water-Gas Shift Reaction",
pages = "175-171",
volume = "757",
doi = "10.4028/www.scientific.net/KEM.757.171"
}

Sangsong, S., Srimala, K., Radonjić, M., Tungkamani, S., Sornchamni, T.,& Phongaksorn, M.. (2017). The Effect of Preparation Method on Ni/Ce/Al Catalyst for High Temperature Water-Gas Shift Reaction. in Key Engineering Materials, 757, 171-175.
https://doi.org/10.4028/www.scientific.net/KEM.757.171

Sangsong S, Srimala K, Radonjić M, Tungkamani S, Sornchamni T, Phongaksorn M. The Effect of Preparation Method on Ni/Ce/Al Catalyst for High Temperature Water-Gas Shift Reaction. in Key Engineering Materials. 2017;757:171-175.
doi:10.4028/www.scientific.net/KEM.757.171 .

Sangsong, S., Srimala, K., Radonjić, Mia, Tungkamani, S., Sornchamni, T., Phongaksorn, M., "The Effect of Preparation Method on Ni/Ce/Al Catalyst for High Temperature Water-Gas Shift Reaction" in Key Engineering Materials, 757 (2017):171-175,
https://doi.org/10.4028/www.scientific.net/KEM.757.171 . .

TY  - CONF
AU  - Radonjić, Mia
AU  - Zvicer, Jovana
AU  - Obradović, Bojana
PY  - 2016
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6349
PB  - Institute of Technical Sciences of the Serbian Academy of Sciences and Arts
C3  - 15th Young Researchers Conference – Materials Science and Engineering Programme and the Book of Abstracts
T1  - Operating conditions in the bioreactor prototype applying  hydrostatic pressures
SP  - 12
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6349
ER  - 

@conference{
author = "Radonjić, Mia and Zvicer, Jovana and Obradović, Bojana",
year = "2016",
publisher = "Institute of Technical Sciences of the Serbian Academy of Sciences and Arts",
journal = "15th Young Researchers Conference – Materials Science and Engineering Programme and the Book of Abstracts",
title = "Operating conditions in the bioreactor prototype applying  hydrostatic pressures",
pages = "12",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6349"
}

Radonjić, M., Zvicer, J.,& Obradović, B.. (2016). Operating conditions in the bioreactor prototype applying  hydrostatic pressures. in 15th Young Researchers Conference – Materials Science and Engineering Programme and the Book of Abstracts
Institute of Technical Sciences of the Serbian Academy of Sciences and Arts., 12.
https://hdl.handle.net/21.15107/rcub_technorep_6349

Radonjić M, Zvicer J, Obradović B. Operating conditions in the bioreactor prototype applying  hydrostatic pressures. in 15th Young Researchers Conference – Materials Science and Engineering Programme and the Book of Abstracts. 2016;:12.
https://hdl.handle.net/21.15107/rcub_technorep_6349 .

Radonjić, Mia, Zvicer, Jovana, Obradović, Bojana, "Operating conditions in the bioreactor prototype applying  hydrostatic pressures" in 15th Young Researchers Conference – Materials Science and Engineering Programme and the Book of Abstracts (2016):12,
https://hdl.handle.net/21.15107/rcub_technorep_6349 .