TY  - CONF
AU  - Stevanović, Milena
AU  - Đošić, Marija
AU  - Janković, Ana
AU  - Sprecher, Christoph Martin
AU  - Vukašinović-Sekulić, Maja
AU  - Mišković-Stanković, Vesna
PY  - 2024
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7442
AB  - Tissue engineering strives for innovative solutions in addressing challenges associated with contemporary bone tissue implants. This study focuses on the electrophoretic deposition (EPD) of hydroxyapatite-based bioceramic composites containing antibacterial agents onto titanium surfaces. Two composite coatings, hydroxyapa-tite/chitosan (HAP/CS) and hydroxyapatite/chitosan/gentamicin (HAP/CS/Gent) were developed to combat issues such as poor adhesion, limited antibacterial potential, limited bioactivity, and potential toxicity of implant materials [1].
PB  - Savez hemijskih inženjera Srbije
C3  - Hemijska industrija - Supplementary Issue - ExcellMater Conference 2024 Abstracts
T1  - Innovative hydroxyapatite-based coatings for bone implants: A multifaceted approach
IS  - 1S
SP  - 58
VL  - 78
UR  - https://hdl.handle.net/21.15107/rcub_technorep_7442
ER  - 

@conference{
author = "Stevanović, Milena and Đošić, Marija and Janković, Ana and Sprecher, Christoph Martin and Vukašinović-Sekulić, Maja and Mišković-Stanković, Vesna",
year = "2024",
abstract = "Tissue engineering strives for innovative solutions in addressing challenges associated with contemporary bone tissue implants. This study focuses on the electrophoretic deposition (EPD) of hydroxyapatite-based bioceramic composites containing antibacterial agents onto titanium surfaces. Two composite coatings, hydroxyapa-tite/chitosan (HAP/CS) and hydroxyapatite/chitosan/gentamicin (HAP/CS/Gent) were developed to combat issues such as poor adhesion, limited antibacterial potential, limited bioactivity, and potential toxicity of implant materials [1].",
publisher = "Savez hemijskih inženjera Srbije",
journal = "Hemijska industrija - Supplementary Issue - ExcellMater Conference 2024 Abstracts",
title = "Innovative hydroxyapatite-based coatings for bone implants: A multifaceted approach",
number = "1S",
pages = "58",
volume = "78",
url = "https://hdl.handle.net/21.15107/rcub_technorep_7442"
}

Stevanović, M., Đošić, M., Janković, A., Sprecher, C. M., Vukašinović-Sekulić, M.,& Mišković-Stanković, V.. (2024). Innovative hydroxyapatite-based coatings for bone implants: A multifaceted approach. in Hemijska industrija - Supplementary Issue - ExcellMater Conference 2024 Abstracts
Savez hemijskih inženjera Srbije., 78(1S), 58.
https://hdl.handle.net/21.15107/rcub_technorep_7442

Stevanović M, Đošić M, Janković A, Sprecher CM, Vukašinović-Sekulić M, Mišković-Stanković V. Innovative hydroxyapatite-based coatings for bone implants: A multifaceted approach. in Hemijska industrija - Supplementary Issue - ExcellMater Conference 2024 Abstracts. 2024;78(1S):58.
https://hdl.handle.net/21.15107/rcub_technorep_7442 .

Stevanović, Milena, Đošić, Marija, Janković, Ana, Sprecher, Christoph Martin, Vukašinović-Sekulić, Maja, Mišković-Stanković, Vesna, "Innovative hydroxyapatite-based coatings for bone implants: A multifaceted approach" in Hemijska industrija - Supplementary Issue - ExcellMater Conference 2024 Abstracts, 78, no. 1S (2024):58,
https://hdl.handle.net/21.15107/rcub_technorep_7442 .

TY  - JOUR
AU  - Vanheuverzwijn, Jérome
AU  - Maillard, Eloise-Eliane
AU  - Mahat, Amal
AU  - Fowler, Lee
AU  - Monteyne, Daniel
AU  - Bonnaud, Leïla
AU  - Landercy, Nicolas
AU  - Hemberg, Axel
AU  - Janković, Ana
AU  - Meyer, Franck
AU  - Mišković-Stanković, Vesna
AU  - Stevanović, Milena
AU  - Mirica, Codruta
AU  - Pérez-Morga, David
AU  - Luginbuehl, Reto
AU  - Combes, Christèle
AU  - Furtos, Gabriel
AU  - Fontaine, Véronique
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6381
AB  - Medical implants have improved the quality of life of many patients. However, surgical intervention may eventually lead to implant microbial contamination. The aims of this research were to develop an easy, robust, quantitative assay to assess surface antimicrobial activities, especially the anti-nascent biofilm activity, and to identify control surfaces, allowing for international comparisons. Using new antimicrobial assays to assess the inhibition of nascent biofilm during persistent contact or after transient contact with bacteria, we show that the 5 cent Euro coin or other metal-based antibacterial coins can be used as positive controls, as more than 4 log reduction on bacterial survival was observed when using either S. aureus or P. aeruginosa as targets. The methods and controls described here could be useful to develop an easy, flexible and standardizable assay to assess relevant antimicrobial activities of new implant materials developed by industries and academics.
PB  - MDPI
T2  - Microorganisms
T1  - Easy, Flexible and Standardizable Anti-Nascent Biofilm Activity Assay to Assess Implant Materials
IS  - 4
SP  - 1023
VL  - 11
DO  - 10.3390/microorganisms11041023
ER  - 

@article{
author = "Vanheuverzwijn, Jérome and Maillard, Eloise-Eliane and Mahat, Amal and Fowler, Lee and Monteyne, Daniel and Bonnaud, Leïla and Landercy, Nicolas and Hemberg, Axel and Janković, Ana and Meyer, Franck and Mišković-Stanković, Vesna and Stevanović, Milena and Mirica, Codruta and Pérez-Morga, David and Luginbuehl, Reto and Combes, Christèle and Furtos, Gabriel and Fontaine, Véronique",
year = "2023",
abstract = "Medical implants have improved the quality of life of many patients. However, surgical intervention may eventually lead to implant microbial contamination. The aims of this research were to develop an easy, robust, quantitative assay to assess surface antimicrobial activities, especially the anti-nascent biofilm activity, and to identify control surfaces, allowing for international comparisons. Using new antimicrobial assays to assess the inhibition of nascent biofilm during persistent contact or after transient contact with bacteria, we show that the 5 cent Euro coin or other metal-based antibacterial coins can be used as positive controls, as more than 4 log reduction on bacterial survival was observed when using either S. aureus or P. aeruginosa as targets. The methods and controls described here could be useful to develop an easy, flexible and standardizable assay to assess relevant antimicrobial activities of new implant materials developed by industries and academics.",
publisher = "MDPI",
journal = "Microorganisms",
title = "Easy, Flexible and Standardizable Anti-Nascent Biofilm Activity Assay to Assess Implant Materials",
number = "4",
pages = "1023",
volume = "11",
doi = "10.3390/microorganisms11041023"
}

Vanheuverzwijn, J., Maillard, E., Mahat, A., Fowler, L., Monteyne, D., Bonnaud, L., Landercy, N., Hemberg, A., Janković, A., Meyer, F., Mišković-Stanković, V., Stevanović, M., Mirica, C., Pérez-Morga, D., Luginbuehl, R., Combes, C., Furtos, G.,& Fontaine, V.. (2023). Easy, Flexible and Standardizable Anti-Nascent Biofilm Activity Assay to Assess Implant Materials. in Microorganisms
MDPI., 11(4), 1023.
https://doi.org/10.3390/microorganisms11041023

Vanheuverzwijn J, Maillard E, Mahat A, Fowler L, Monteyne D, Bonnaud L, Landercy N, Hemberg A, Janković A, Meyer F, Mišković-Stanković V, Stevanović M, Mirica C, Pérez-Morga D, Luginbuehl R, Combes C, Furtos G, Fontaine V. Easy, Flexible and Standardizable Anti-Nascent Biofilm Activity Assay to Assess Implant Materials. in Microorganisms. 2023;11(4):1023.
doi:10.3390/microorganisms11041023 .

Vanheuverzwijn, Jérome, Maillard, Eloise-Eliane, Mahat, Amal, Fowler, Lee, Monteyne, Daniel, Bonnaud, Leïla, Landercy, Nicolas, Hemberg, Axel, Janković, Ana, Meyer, Franck, Mišković-Stanković, Vesna, Stevanović, Milena, Mirica, Codruta, Pérez-Morga, David, Luginbuehl, Reto, Combes, Christèle, Furtos, Gabriel, Fontaine, Véronique, "Easy, Flexible and Standardizable Anti-Nascent Biofilm Activity Assay to Assess Implant Materials" in Microorganisms, 11, no. 4 (2023):1023,
https://doi.org/10.3390/microorganisms11041023 . .

TY  - JOUR
AU  - Radonjić, Mia
AU  - Petrović, Jelena
AU  - Milivojević, Milena
AU  - Stevanović, Milena
AU  - Stojkovska, Jasmina
AU  - Obradović, Bojana
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5261
AB  - A multidisciplinary approach based on experiments and mathematical modeling was used in biomimetic system development for three-dimensional (3D) cultures of cancer cells. Specifically, two cancer cell lines, human embryonic teratocarcinoma NT2/D1 and rat glioma C6, were immobilized in alginate microbeads and microfibers, respectively, and cultured under static and flow conditions in perfusion bioreactors. At the same time, chemical engineering methods were applied to explain the obtained results. The superficial medium velocity of 80 μm s-1 induced lower viability of NT2/D1 cells in superficial microbead zones, implying adverse effects of fluid shear stresses estimated as ∼67 mPa. On the contrary, similar velocity (100 μm s-1) enhanced the proliferation of C6 glioma cells within microfibers compared to static controls. An additional study of silver release from nanocomposite Ag/honey/alginate microfibers under perfusion indicated that the medium partially flows through the hydrogel (interstitial velocity of ∼10 nm s-1). Thus, a diffusion-advection-reaction model described the mass transport to immobilized cells within microfibers. Substances with diffusion coefficients of ∼10-9-10-11 m2 s-1 are sufficiently supplied by diffusion only, while those with significantly lower diffusivities (∼10-19 m2 s-1) require additional convective transport. The present study demonstrates the selection and contribution of chemical engineering methods in tumor model system development.
PB  - National Library of Serbia
T2  - Chemical Industry and Chemical Engineering Quarterly
T2  - Chemical Industry and Chemical Engineering Quarterly
T1  - Chemical engineering methods in analyses of 3D cancer cell cultures: Hydrodinamic and mass transport considerations
EP  - 223
IS  - 3
SP  - 211
VL  - 28
DO  - 10.2298/CICEQ210607033R
ER  - 

@article{
author = "Radonjić, Mia and Petrović, Jelena and Milivojević, Milena and Stevanović, Milena and Stojkovska, Jasmina and Obradović, Bojana",
year = "2022",
abstract = "A multidisciplinary approach based on experiments and mathematical modeling was used in biomimetic system development for three-dimensional (3D) cultures of cancer cells. Specifically, two cancer cell lines, human embryonic teratocarcinoma NT2/D1 and rat glioma C6, were immobilized in alginate microbeads and microfibers, respectively, and cultured under static and flow conditions in perfusion bioreactors. At the same time, chemical engineering methods were applied to explain the obtained results. The superficial medium velocity of 80 μm s-1 induced lower viability of NT2/D1 cells in superficial microbead zones, implying adverse effects of fluid shear stresses estimated as ∼67 mPa. On the contrary, similar velocity (100 μm s-1) enhanced the proliferation of C6 glioma cells within microfibers compared to static controls. An additional study of silver release from nanocomposite Ag/honey/alginate microfibers under perfusion indicated that the medium partially flows through the hydrogel (interstitial velocity of ∼10 nm s-1). Thus, a diffusion-advection-reaction model described the mass transport to immobilized cells within microfibers. Substances with diffusion coefficients of ∼10-9-10-11 m2 s-1 are sufficiently supplied by diffusion only, while those with significantly lower diffusivities (∼10-19 m2 s-1) require additional convective transport. The present study demonstrates the selection and contribution of chemical engineering methods in tumor model system development.",
publisher = "National Library of Serbia",
journal = "Chemical Industry and Chemical Engineering Quarterly, Chemical Industry and Chemical Engineering Quarterly",
title = "Chemical engineering methods in analyses of 3D cancer cell cultures: Hydrodinamic and mass transport considerations",
pages = "223-211",
number = "3",
volume = "28",
doi = "10.2298/CICEQ210607033R"
}

Radonjić, M., Petrović, J., Milivojević, M., Stevanović, M., Stojkovska, J.,& Obradović, B.. (2022). Chemical engineering methods in analyses of 3D cancer cell cultures: Hydrodinamic and mass transport considerations. in Chemical Industry and Chemical Engineering Quarterly
National Library of Serbia., 28(3), 211-223.
https://doi.org/10.2298/CICEQ210607033R

Radonjić M, Petrović J, Milivojević M, Stevanović M, Stojkovska J, Obradović B. Chemical engineering methods in analyses of 3D cancer cell cultures: Hydrodinamic and mass transport considerations. in Chemical Industry and Chemical Engineering Quarterly. 2022;28(3):211-223.
doi:10.2298/CICEQ210607033R .

Radonjić, Mia, Petrović, Jelena, Milivojević, Milena, Stevanović, Milena, Stojkovska, Jasmina, Obradović, Bojana, "Chemical engineering methods in analyses of 3D cancer cell cultures: Hydrodinamic and mass transport considerations" in Chemical Industry and Chemical Engineering Quarterly, 28, no. 3 (2022):211-223,
https://doi.org/10.2298/CICEQ210607033R . .

TY  - CONF
AU  - Milošević, Mia
AU  - Banićević, Ivana
AU  - Pavlović, Marija
AU  - Milivojević, Milena
AU  - Stevanović, Milena
AU  - Stojkovska, Jasmina
AU  - Obradović, Bojana
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6351
PB  - Institute of Technical Sciences of SASA
C3  - Twentieth Young Researchers’ Conference - Materials Science and Engineering: Programme and the Book of Abstracts
T1  - Development of a physiologically relevant osteosarcoma model based on alginate scaffolds and perfusion bioreactor
SP  - 8
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6351
ER  - 

@conference{
author = "Milošević, Mia and Banićević, Ivana and Pavlović, Marija and Milivojević, Milena and Stevanović, Milena and Stojkovska, Jasmina and Obradović, Bojana",
year = "2022",
publisher = "Institute of Technical Sciences of SASA",
journal = "Twentieth Young Researchers’ Conference - Materials Science and Engineering: Programme and the Book of Abstracts",
title = "Development of a physiologically relevant osteosarcoma model based on alginate scaffolds and perfusion bioreactor",
pages = "8",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6351"
}

Milošević, M., Banićević, I., Pavlović, M., Milivojević, M., Stevanović, M., Stojkovska, J.,& Obradović, B.. (2022). Development of a physiologically relevant osteosarcoma model based on alginate scaffolds and perfusion bioreactor. in Twentieth Young Researchers’ Conference - Materials Science and Engineering: Programme and the Book of Abstracts
Institute of Technical Sciences of SASA., 8.
https://hdl.handle.net/21.15107/rcub_technorep_6351

Milošević M, Banićević I, Pavlović M, Milivojević M, Stevanović M, Stojkovska J, Obradović B. Development of a physiologically relevant osteosarcoma model based on alginate scaffolds and perfusion bioreactor. in Twentieth Young Researchers’ Conference - Materials Science and Engineering: Programme and the Book of Abstracts. 2022;:8.
https://hdl.handle.net/21.15107/rcub_technorep_6351 .

Milošević, Mia, Banićević, Ivana, Pavlović, Marija, Milivojević, Milena, Stevanović, Milena, Stojkovska, Jasmina, Obradović, Bojana, "Development of a physiologically relevant osteosarcoma model based on alginate scaffolds and perfusion bioreactor" in Twentieth Young Researchers’ Conference - Materials Science and Engineering: Programme and the Book of Abstracts (2022):8,
https://hdl.handle.net/21.15107/rcub_technorep_6351 .

TY  - CONF
AU  - Banićević, Ivana
AU  - Menshikh, Ksenia
AU  - Radonjić, Mia
AU  - Petrović, Jelena
AU  - Janković, Radmila
AU  - Milivojević, Milena
AU  - Stevanović, Milena
AU  - Stojkovska, Jasmina
AU  - Obradović, Bojana
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6350
PB  - Institute of Technical Sciences of SASA
C3  - Twentieth Young Researchers’ Conference - Materials Science and Engineering: Programme and the Book of Abstracts
T1  - Cellular self-assembly in a 3D osteosarcoma culture model based on alginate scaffolds and perfusion bioreactor
SP  - 9
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6350
ER  - 

@conference{
author = "Banićević, Ivana and Menshikh, Ksenia and Radonjić, Mia and Petrović, Jelena and Janković, Radmila and Milivojević, Milena and Stevanović, Milena and Stojkovska, Jasmina and Obradović, Bojana",
year = "2022",
publisher = "Institute of Technical Sciences of SASA",
journal = "Twentieth Young Researchers’ Conference - Materials Science and Engineering: Programme and the Book of Abstracts",
title = "Cellular self-assembly in a 3D osteosarcoma culture model based on alginate scaffolds and perfusion bioreactor",
pages = "9",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6350"
}

Banićević, I., Menshikh, K., Radonjić, M., Petrović, J., Janković, R., Milivojević, M., Stevanović, M., Stojkovska, J.,& Obradović, B.. (2022). Cellular self-assembly in a 3D osteosarcoma culture model based on alginate scaffolds and perfusion bioreactor. in Twentieth Young Researchers’ Conference - Materials Science and Engineering: Programme and the Book of Abstracts
Institute of Technical Sciences of SASA., 9.
https://hdl.handle.net/21.15107/rcub_technorep_6350

Banićević I, Menshikh K, Radonjić M, Petrović J, Janković R, Milivojević M, Stevanović M, Stojkovska J, Obradović B. Cellular self-assembly in a 3D osteosarcoma culture model based on alginate scaffolds and perfusion bioreactor. in Twentieth Young Researchers’ Conference - Materials Science and Engineering: Programme and the Book of Abstracts. 2022;:9.
https://hdl.handle.net/21.15107/rcub_technorep_6350 .

Banićević, Ivana, Menshikh, Ksenia, Radonjić, Mia, Petrović, Jelena, Janković, Radmila, Milivojević, Milena, Stevanović, Milena, Stojkovska, Jasmina, Obradović, Bojana, "Cellular self-assembly in a 3D osteosarcoma culture model based on alginate scaffolds and perfusion bioreactor" in Twentieth Young Researchers’ Conference - Materials Science and Engineering: Programme and the Book of Abstracts (2022):9,
https://hdl.handle.net/21.15107/rcub_technorep_6350 .

TY  - CONF
AU  - Banićević, Ivana
AU  - Radonjić, Mia
AU  - Pavlović, Marija
AU  - Milivojević, Milena
AU  - Stevanović, Milena
AU  - Stojkovska, Jasmina
AU  - Obradović, Bojana
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5028
AB  - Osteosarcoma is the most common type of bone cancer, which affects both children and
adults. Treatment of osteosarcoma exhibits slow progress due to inadequacy of both in vivo
animal models and 2D in vitro models regularly used for antitumor drug testing. Our
approach is to create a physiologically relevant 3D in vitro model for osteosarcoma cell
cultivation, which has the potential to overcome inherent weaknesses of 2D in vitro and
animal models. In order to imitate native osteosarcoma microenvironment, macroporous
alginate scaffolds with incorporated hydroxyapatite/β-tricalcium phosphate (HAp/β-TCP)
powder were produced with two compositions: 1 wt% alginate, 1 wt% powder and 2 wt.%
alginate, 2 wt% powder. Bioactivity and stability of the scaffolds were investigated under
biomimetic conditions of continuous flow of the culture medium in perfusion bioreactor at
the superficial medium velocity of 400 µm/s, which was reported in literature to be
beneficial for osteogenesis. Scaffolds with the higher alginate concentration was shown to be
more stable in the culture medium, since the scaffolds with the lower alginate concentration
disintegrated after 5-7 days under flow conditions. Biocompatibility of the obtained scaffolds
was investigated in short-term cultivation studies of murine osteosarcoma cells K7M2-wt
seeded onto the scaffolds. The scaffolds were cultivated in perfusion bioreactors at the
superficial flow velocity of 15 µm/s, while static cultures served as a control. After
cultivation, osteosarcoma cells remained adhered to the scaffold surface, expressed
metabolic activity and retained their initial proliferation ability while the flow was shown to
positively affect the cultivated cells.
PB  - Institute of Technical Sciences of SASA
C3  - Nineteenth Young Researchers’ Conference - Materials Science and Engineering: Program and the Book of Abstracts
T1  - Development of a physiologically relevant 3D in vitro model for osteosarcoma cell cultivation comprising alginate composite scaffolds and a perfusion bioreactor system
SP  - 6
VL  - 17
UR  - https://hdl.handle.net/21.15107/rcub_technorep_5028
ER  - 

@conference{
author = "Banićević, Ivana and Radonjić, Mia and Pavlović, Marija and Milivojević, Milena and Stevanović, Milena and Stojkovska, Jasmina and Obradović, Bojana",
year = "2021",
abstract = "Osteosarcoma is the most common type of bone cancer, which affects both children and
adults. Treatment of osteosarcoma exhibits slow progress due to inadequacy of both in vivo
animal models and 2D in vitro models regularly used for antitumor drug testing. Our
approach is to create a physiologically relevant 3D in vitro model for osteosarcoma cell
cultivation, which has the potential to overcome inherent weaknesses of 2D in vitro and
animal models. In order to imitate native osteosarcoma microenvironment, macroporous
alginate scaffolds with incorporated hydroxyapatite/β-tricalcium phosphate (HAp/β-TCP)
powder were produced with two compositions: 1 wt% alginate, 1 wt% powder and 2 wt.%
alginate, 2 wt% powder. Bioactivity and stability of the scaffolds were investigated under
biomimetic conditions of continuous flow of the culture medium in perfusion bioreactor at
the superficial medium velocity of 400 µm/s, which was reported in literature to be
beneficial for osteogenesis. Scaffolds with the higher alginate concentration was shown to be
more stable in the culture medium, since the scaffolds with the lower alginate concentration
disintegrated after 5-7 days under flow conditions. Biocompatibility of the obtained scaffolds
was investigated in short-term cultivation studies of murine osteosarcoma cells K7M2-wt
seeded onto the scaffolds. The scaffolds were cultivated in perfusion bioreactors at the
superficial flow velocity of 15 µm/s, while static cultures served as a control. After
cultivation, osteosarcoma cells remained adhered to the scaffold surface, expressed
metabolic activity and retained their initial proliferation ability while the flow was shown to
positively affect the cultivated cells.",
publisher = "Institute of Technical Sciences of SASA",
journal = "Nineteenth Young Researchers’ Conference - Materials Science and Engineering: Program and the Book of Abstracts",
title = "Development of a physiologically relevant 3D in vitro model for osteosarcoma cell cultivation comprising alginate composite scaffolds and a perfusion bioreactor system",
pages = "6",
volume = "17",
url = "https://hdl.handle.net/21.15107/rcub_technorep_5028"
}

Banićević, I., Radonjić, M., Pavlović, M., Milivojević, M., Stevanović, M., Stojkovska, J.,& Obradović, B.. (2021). Development of a physiologically relevant 3D in vitro model for osteosarcoma cell cultivation comprising alginate composite scaffolds and a perfusion bioreactor system. in Nineteenth Young Researchers’ Conference - Materials Science and Engineering: Program and the Book of Abstracts
Institute of Technical Sciences of SASA., 17, 6.
https://hdl.handle.net/21.15107/rcub_technorep_5028

Banićević I, Radonjić M, Pavlović M, Milivojević M, Stevanović M, Stojkovska J, Obradović B. Development of a physiologically relevant 3D in vitro model for osteosarcoma cell cultivation comprising alginate composite scaffolds and a perfusion bioreactor system. in Nineteenth Young Researchers’ Conference - Materials Science and Engineering: Program and the Book of Abstracts. 2021;17:6.
https://hdl.handle.net/21.15107/rcub_technorep_5028 .

Banićević, Ivana, Radonjić, Mia, Pavlović, Marija, Milivojević, Milena, Stevanović, Milena, Stojkovska, Jasmina, Obradović, Bojana, "Development of a physiologically relevant 3D in vitro model for osteosarcoma cell cultivation comprising alginate composite scaffolds and a perfusion bioreactor system" in Nineteenth Young Researchers’ Conference - Materials Science and Engineering: Program and the Book of Abstracts, 17 (2021):6,
https://hdl.handle.net/21.15107/rcub_technorep_5028 .

TY  - CONF
AU  - Radonjić, Mia
AU  - Petrović, Jelena
AU  - Milivojević, Milena
AU  - Stevanović, Milena
AU  - Stojkovska, Jasmina
AU  - Obradović, Bojana
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6354
AB  - Cancer cell immobilization in polymer hydrogels serving as extracellular matrices and cultivation in perfusion bioreactors
that provide appropriate chemical signals, efficient mass transfer and hydrodynamic shear stresses is a promising strategy
for development of physiologically relevant tumor models. In this work, perfusion cultures of 2 cancer cell types (C6 rat
glioma and embryonal carcinoma NT2/D1 cells) immobilized in alginate microgels were established, while static cultures
served as controls. Continuous perfusion had different effects on the cultured cells inducing enhanced proliferation of the
glioma cells immobilized in microfibers (8x10^6 cell/ml), while reducing the viability of the NT2/D1 cells immobilized in
microbeads (1x10^6 cell/ml). In order to elucidate the observed effects, chemical engineering principles were applied to
assess mass transfer and hydrodynamic conditions. The second Fick’s law was solved analytically while the diffusionadvection-reaction equation was solved numerically to model mass transport in the static and bioreactor cultures,
respectively. Moreover, Reynolds numbers, pressure drops and shear stresses in bioreactor cultures were calculated for
assessment of flow regime and hydrodynamic conditions. The modeling results have indicated that oxygen transport is
diffusion-controlled through the alginate hydrogel, while medium perfusion improves mass transfer of larger compounds
having smaller diffusion coefficients (∼10^(-13) m^2/s), which possibly stimulated glioma cell proliferation. On the other
hand, the obtained shear stress (~50 mPa) in the perfused packed bed of microbeads was above physiological levels, which
provided the explanation of the poor NT2/D1 cell survival. This study stresses the importance of multidisciplinary approach
in addressing such multifactorial diseases as cancer.
C3  - 6th World TERMIS Conference 2021 Abstract Book
T1  - Optimization of 3D cancer cell culture conditions by application of chemical engineering principles
SP  - 1408
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6354
ER  - 

@conference{
author = "Radonjić, Mia and Petrović, Jelena and Milivojević, Milena and Stevanović, Milena and Stojkovska, Jasmina and Obradović, Bojana",
year = "2021",
abstract = "Cancer cell immobilization in polymer hydrogels serving as extracellular matrices and cultivation in perfusion bioreactors
that provide appropriate chemical signals, efficient mass transfer and hydrodynamic shear stresses is a promising strategy
for development of physiologically relevant tumor models. In this work, perfusion cultures of 2 cancer cell types (C6 rat
glioma and embryonal carcinoma NT2/D1 cells) immobilized in alginate microgels were established, while static cultures
served as controls. Continuous perfusion had different effects on the cultured cells inducing enhanced proliferation of the
glioma cells immobilized in microfibers (8x10^6 cell/ml), while reducing the viability of the NT2/D1 cells immobilized in
microbeads (1x10^6 cell/ml). In order to elucidate the observed effects, chemical engineering principles were applied to
assess mass transfer and hydrodynamic conditions. The second Fick’s law was solved analytically while the diffusionadvection-reaction equation was solved numerically to model mass transport in the static and bioreactor cultures,
respectively. Moreover, Reynolds numbers, pressure drops and shear stresses in bioreactor cultures were calculated for
assessment of flow regime and hydrodynamic conditions. The modeling results have indicated that oxygen transport is
diffusion-controlled through the alginate hydrogel, while medium perfusion improves mass transfer of larger compounds
having smaller diffusion coefficients (∼10^(-13) m^2/s), which possibly stimulated glioma cell proliferation. On the other
hand, the obtained shear stress (~50 mPa) in the perfused packed bed of microbeads was above physiological levels, which
provided the explanation of the poor NT2/D1 cell survival. This study stresses the importance of multidisciplinary approach
in addressing such multifactorial diseases as cancer.",
journal = "6th World TERMIS Conference 2021 Abstract Book",
title = "Optimization of 3D cancer cell culture conditions by application of chemical engineering principles",
pages = "1408",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6354"
}

Radonjić, M., Petrović, J., Milivojević, M., Stevanović, M., Stojkovska, J.,& Obradović, B.. (2021). Optimization of 3D cancer cell culture conditions by application of chemical engineering principles. in 6th World TERMIS Conference 2021 Abstract Book, 1408.
https://hdl.handle.net/21.15107/rcub_technorep_6354

Radonjić M, Petrović J, Milivojević M, Stevanović M, Stojkovska J, Obradović B. Optimization of 3D cancer cell culture conditions by application of chemical engineering principles. in 6th World TERMIS Conference 2021 Abstract Book. 2021;:1408.
https://hdl.handle.net/21.15107/rcub_technorep_6354 .

Radonjić, Mia, Petrović, Jelena, Milivojević, Milena, Stevanović, Milena, Stojkovska, Jasmina, Obradović, Bojana, "Optimization of 3D cancer cell culture conditions by application of chemical engineering principles" in 6th World TERMIS Conference 2021 Abstract Book (2021):1408,
https://hdl.handle.net/21.15107/rcub_technorep_6354 .

TY  - JOUR
AU  - Stojkovska, Jasmina
AU  - Zvicer, Jovana
AU  - Milivojević, Milena
AU  - Petrović, Isidora
AU  - Stevanović, Milena
AU  - Obradović, Bojana
PY  - 2020
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4373
AB  - Development of drugs is a complex, time- and cost-consuming process due to the lack of standardized and reliable characterization techniques and models. Traditionally, drug screening is based on in vitro analysis using two-dimensional (2D) cell cultures followed by in vivo animal testing. Unfortunately, application of the obtained results to humans in about 90 % of cases fails. Therefore, it is important to develop and improve cell-based systems that can mimic the in vivo-like conditions to provide more reliable results. In this paper, we present development and validation of a novel, user-friendly perfusion bioreactor system for single use aimed for cancer research, drug screening, anti-cancer drug response studies, biomaterial characterization, and tissue engineering. Simple design of the perfusion bioreactor provides direct medium flow at physiological velocities (100-250 mu m s(-1)) through samples of different sizes and shapes. Biocompatibility of the bioreactor was confirmed in short term cultivation studies of cervical carcinoma SiHa cells immobilized in alginate microfibers under continuous medium flow. The results have shown preserved cell viability indicating that the perfusion bioreactor in conjunction with alginate hydrogels as cell carriers could be potentially used as a tool for controlled anti-cancer drug screening in a 3D environment.
PB  - Savez hemijskih inženjera, Beograd
T2  - Hemijska industrija
T1  - Validation of a novel perfusion bioreactor system in cancer research
EP  - 196
IS  - 3
SP  - 187
VL  - 74
DO  - 10.2298/HEMIND200329015S
ER  - 

@article{
author = "Stojkovska, Jasmina and Zvicer, Jovana and Milivojević, Milena and Petrović, Isidora and Stevanović, Milena and Obradović, Bojana",
year = "2020",
abstract = "Development of drugs is a complex, time- and cost-consuming process due to the lack of standardized and reliable characterization techniques and models. Traditionally, drug screening is based on in vitro analysis using two-dimensional (2D) cell cultures followed by in vivo animal testing. Unfortunately, application of the obtained results to humans in about 90 % of cases fails. Therefore, it is important to develop and improve cell-based systems that can mimic the in vivo-like conditions to provide more reliable results. In this paper, we present development and validation of a novel, user-friendly perfusion bioreactor system for single use aimed for cancer research, drug screening, anti-cancer drug response studies, biomaterial characterization, and tissue engineering. Simple design of the perfusion bioreactor provides direct medium flow at physiological velocities (100-250 mu m s(-1)) through samples of different sizes and shapes. Biocompatibility of the bioreactor was confirmed in short term cultivation studies of cervical carcinoma SiHa cells immobilized in alginate microfibers under continuous medium flow. The results have shown preserved cell viability indicating that the perfusion bioreactor in conjunction with alginate hydrogels as cell carriers could be potentially used as a tool for controlled anti-cancer drug screening in a 3D environment.",
publisher = "Savez hemijskih inženjera, Beograd",
journal = "Hemijska industrija",
title = "Validation of a novel perfusion bioreactor system in cancer research",
pages = "196-187",
number = "3",
volume = "74",
doi = "10.2298/HEMIND200329015S"
}

Stojkovska, J., Zvicer, J., Milivojević, M., Petrović, I., Stevanović, M.,& Obradović, B.. (2020). Validation of a novel perfusion bioreactor system in cancer research. in Hemijska industrija
Savez hemijskih inženjera, Beograd., 74(3), 187-196.
https://doi.org/10.2298/HEMIND200329015S

Stojkovska J, Zvicer J, Milivojević M, Petrović I, Stevanović M, Obradović B. Validation of a novel perfusion bioreactor system in cancer research. in Hemijska industrija. 2020;74(3):187-196.
doi:10.2298/HEMIND200329015S .

Stojkovska, Jasmina, Zvicer, Jovana, Milivojević, Milena, Petrović, Isidora, Stevanović, Milena, Obradović, Bojana, "Validation of a novel perfusion bioreactor system in cancer research" in Hemijska industrija, 74, no. 3 (2020):187-196,
https://doi.org/10.2298/HEMIND200329015S . .