TY  - JOUR
AU  - Lukić, Ivana
AU  - Milovanović, Stoja
AU  - Pantić, Milica
AU  - Srbljak, Ivana
AU  - Djurić, Ana
AU  - Tadić, Vanja
AU  - Tyśkiewicz, Katarzyna
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5093
AB  - Extracts from Silybum marianum seeds have high industrial potential due to their application as phytopharmaceuticals and food. In order to optimize its production, conventional extraction (in Soxhlet apparatus using solvents ethanol and n-hexane) and supercritical fluid extraction (using CO2 at the pressure of 30 MPa and temperature of 40 °C with and without co-solvent ethanol, from oil-rich and defatted seeds) were compared. In addition, the effect of plant cultivation (years 2011 and 2019) and the effect of seeds and extract storage on the yield and quality of extracts were assessed. It was shown that unsaturated fatty acids (linoleic and oleic acids being most dominant) constituted 64–87% of obtained extracts. Content of α-tocopherol varied from 0.01 to 13 g/100 g, while total phenolic content was in the range from 13.2 to 104.2 gGAE/100 gextract. In vitro cytotoxic activity analysis confirmed that extracts obtained from defatted seeds showed activity against cancer cells. This study pointed out the important role of the cultivation year, selection of extraction technique and solvent, as well as storage on the extraction yield, chemical profile, and cytotoxic activity of extracts.
PB  - Academic Press
T2  - LWT
T1  - Separation of high-value extracts from Silybum marianum seeds: Influence of extraction technique and storage on composition and bioactivity
SP  - 113319
VL  - 160
DO  - 10.1016/j.lwt.2022.113319
ER  - 

@article{
author = "Lukić, Ivana and Milovanović, Stoja and Pantić, Milica and Srbljak, Ivana and Djurić, Ana and Tadić, Vanja and Tyśkiewicz, Katarzyna",
year = "2022",
abstract = "Extracts from Silybum marianum seeds have high industrial potential due to their application as phytopharmaceuticals and food. In order to optimize its production, conventional extraction (in Soxhlet apparatus using solvents ethanol and n-hexane) and supercritical fluid extraction (using CO2 at the pressure of 30 MPa and temperature of 40 °C with and without co-solvent ethanol, from oil-rich and defatted seeds) were compared. In addition, the effect of plant cultivation (years 2011 and 2019) and the effect of seeds and extract storage on the yield and quality of extracts were assessed. It was shown that unsaturated fatty acids (linoleic and oleic acids being most dominant) constituted 64–87% of obtained extracts. Content of α-tocopherol varied from 0.01 to 13 g/100 g, while total phenolic content was in the range from 13.2 to 104.2 gGAE/100 gextract. In vitro cytotoxic activity analysis confirmed that extracts obtained from defatted seeds showed activity against cancer cells. This study pointed out the important role of the cultivation year, selection of extraction technique and solvent, as well as storage on the extraction yield, chemical profile, and cytotoxic activity of extracts.",
publisher = "Academic Press",
journal = "LWT",
title = "Separation of high-value extracts from Silybum marianum seeds: Influence of extraction technique and storage on composition and bioactivity",
pages = "113319",
volume = "160",
doi = "10.1016/j.lwt.2022.113319"
}

Lukić, I., Milovanović, S., Pantić, M., Srbljak, I., Djurić, A., Tadić, V.,& Tyśkiewicz, K.. (2022). Separation of high-value extracts from Silybum marianum seeds: Influence of extraction technique and storage on composition and bioactivity. in LWT
Academic Press., 160, 113319.
https://doi.org/10.1016/j.lwt.2022.113319

Lukić I, Milovanović S, Pantić M, Srbljak I, Djurić A, Tadić V, Tyśkiewicz K. Separation of high-value extracts from Silybum marianum seeds: Influence of extraction technique and storage on composition and bioactivity. in LWT. 2022;160:113319.
doi:10.1016/j.lwt.2022.113319 .

Lukić, Ivana, Milovanović, Stoja, Pantić, Milica, Srbljak, Ivana, Djurić, Ana, Tadić, Vanja, Tyśkiewicz, Katarzyna, "Separation of high-value extracts from Silybum marianum seeds: Influence of extraction technique and storage on composition and bioactivity" in LWT, 160 (2022):113319,
https://doi.org/10.1016/j.lwt.2022.113319 . .

TY  - CONF
AU  - Milovanović, Stoja
AU  - Pantić, Milica
AU  - Pavlović, Stefan M.
AU  - Novak, Zoran
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6903
AB  - Polylactide (PLA) is a versatile polymer with a variety of applications from consumer goods (e.g. disposable cutlery, food, and beverage packaging) to medical (e.g. disposable protective garments, implants), agriculture (e.g. mulch films), etc [1]. However, its use as porous materials is still limited [2]. In order to increase possible PLA-based material applications, the present work was focused on the preparation of PLA aerogels with defined morphology. For
this purpose, three polymer solvents were tested (chloroform, dichloromethane, and dioxane). PLA solutions, prepared at room temperature, were immersed in antisolvent (absolute ethanol) for solvent replacement prior to drying. Obtained alcogels were dried using supercritical CO
2 at 150 bar and 35 °C in a combination of static and dynamic regimes up to 3 h [3] for aerogel production. While chloroform and dichloromethane enabled the production of stable material, the use of dioxane as a solvent led to the formation of aerogels that were crumbling during retrieving from a high-pressure drying unit. Stable aerogels were further characterized using scanning electron microscopy, water displacement method, mercury intrusion porosimetry, gas adsorption-desorption analysis as well as Fourier transform infrared spectroscopy and thermogravimetry combined with differential scanning calorimetry. After the selection of optimal solvent, two temperatures for solvent replacement with anti-solvent ethanol were compared (-20 and 22 °C). Results showed that both solvent selection as well as temperature for solvent replacement have a significant effect on the morphology of PLA aerogels. The density of aerogels ranged from 0.34 to 0.47 g/cm3 while its porosity ranged from 62.6 to 73.0% [3].
C3  - 2nd International Conference on Aerogels for Biomedical and Environmental Applications (AERoGELS2022), 29 June–1 July 2022, Athens, Greece
T1  - Influence of solvent selection and temperature for solvent replacement on the morphology of PLA aerogels
SP  - P-24
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6903
ER  - 

@conference{
author = "Milovanović, Stoja and Pantić, Milica and Pavlović, Stefan M. and Novak, Zoran",
year = "2022",
abstract = "Polylactide (PLA) is a versatile polymer with a variety of applications from consumer goods (e.g. disposable cutlery, food, and beverage packaging) to medical (e.g. disposable protective garments, implants), agriculture (e.g. mulch films), etc [1]. However, its use as porous materials is still limited [2]. In order to increase possible PLA-based material applications, the present work was focused on the preparation of PLA aerogels with defined morphology. For
this purpose, three polymer solvents were tested (chloroform, dichloromethane, and dioxane). PLA solutions, prepared at room temperature, were immersed in antisolvent (absolute ethanol) for solvent replacement prior to drying. Obtained alcogels were dried using supercritical CO
2 at 150 bar and 35 °C in a combination of static and dynamic regimes up to 3 h [3] for aerogel production. While chloroform and dichloromethane enabled the production of stable material, the use of dioxane as a solvent led to the formation of aerogels that were crumbling during retrieving from a high-pressure drying unit. Stable aerogels were further characterized using scanning electron microscopy, water displacement method, mercury intrusion porosimetry, gas adsorption-desorption analysis as well as Fourier transform infrared spectroscopy and thermogravimetry combined with differential scanning calorimetry. After the selection of optimal solvent, two temperatures for solvent replacement with anti-solvent ethanol were compared (-20 and 22 °C). Results showed that both solvent selection as well as temperature for solvent replacement have a significant effect on the morphology of PLA aerogels. The density of aerogels ranged from 0.34 to 0.47 g/cm3 while its porosity ranged from 62.6 to 73.0% [3].",
journal = "2nd International Conference on Aerogels for Biomedical and Environmental Applications (AERoGELS2022), 29 June–1 July 2022, Athens, Greece",
title = "Influence of solvent selection and temperature for solvent replacement on the morphology of PLA aerogels",
pages = "P-24",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6903"
}

Milovanović, S., Pantić, M., Pavlović, S. M.,& Novak, Z.. (2022). Influence of solvent selection and temperature for solvent replacement on the morphology of PLA aerogels. in 2nd International Conference on Aerogels for Biomedical and Environmental Applications (AERoGELS2022), 29 June–1 July 2022, Athens, Greece, P-24.
https://hdl.handle.net/21.15107/rcub_technorep_6903

Milovanović S, Pantić M, Pavlović SM, Novak Z. Influence of solvent selection and temperature for solvent replacement on the morphology of PLA aerogels. in 2nd International Conference on Aerogels for Biomedical and Environmental Applications (AERoGELS2022), 29 June–1 July 2022, Athens, Greece. 2022;:P-24.
https://hdl.handle.net/21.15107/rcub_technorep_6903 .

Milovanović, Stoja, Pantić, Milica, Pavlović, Stefan M., Novak, Zoran, "Influence of solvent selection and temperature for solvent replacement on the morphology of PLA aerogels" in 2nd International Conference on Aerogels for Biomedical and Environmental Applications (AERoGELS2022), 29 June–1 July 2022, Athens, Greece (2022):P-24,
https://hdl.handle.net/21.15107/rcub_technorep_6903 .

TY  - JOUR
AU  - Milovanović, Stoja
AU  - Pantić, Milica M.
AU  - Ivanović, Jasna
AU  - Žižović, Irena
PY  - 2015
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2908
AB  - The impregnation of differently prepared chitosan gels with thymol using supercritical carbon dioxide (scCO2) was studied in order to obtain new materials with added value without the use of organic solvents. The optimum conditions of supercritical impregnation (15 MPa, 35 °C and 24 h) were defined based on previously investigated thymol solubility in scCO2. Cross-linkers formaldehyde and glutardialdehyde were used to prepare chitosan hydrogels. The obtained hydrogels of chitosan were converted in acetogels and then dried with air or by using scCO2 in order to obtain xerogels and aerogels. The best results, in terms of morphology and yield of impregnation with thymol, have been achieved for the aerogel of chitosan (11.30%) which was obtained with cross-linker formaldehyde and dried with scCO2 at 15 MPa and 50 °C.
AB  - U radu je ispitivana impregnacija različito pripremljenih hitozan gelova sa timolom pomoću natkritičnog ugljenik(IV)-oksida (nkCO2) u cilju dobijanja novih materijala sa dodatom vrednošću bez upotrebe organskih rastvarača. Optimalni uslovi natkritične impregnacije (15 MPa, 35 °C i 24 h) su definisani na osnovu prethodno ispitane rastvorljivosti timola u nkCO2. Za pripremu hidrogelova hitozana korišćeni su umreživači formaldehid i glutardialdehid. Dobijeni hidrogelovi hitozana su prevedeni u acetogelove, a potom sušeni na vazduhu ili pomoću nkCO2 kako bi se dobili kserogelovi ili aerogelovi. Najbolji rezultati u pogledu morfologije i prinosa impregancije sa timolom postignuti su za aerogel hitozana (11,30%) koji je dobijen umrežavanjem sa formaldehidom i sušenjem sa nkCO2 na 15 MPa i 50 °C.
PB  - Savez inženjera i tehničara Srbije, Beograd
T2  - Tehnika
T1  - Optimization of chitosan gel preparation for supercritical impregnation of thymol
T1  - Uticaj pripreme hitozan gelova na natkritičnu impregnaciju sa timolom
EP  - 396
IS  - 3
SP  - 391
VL  - 70
DO  - 10.5937/tehnika1503391M
ER  - 

@article{
author = "Milovanović, Stoja and Pantić, Milica M. and Ivanović, Jasna and Žižović, Irena",
year = "2015",
abstract = "The impregnation of differently prepared chitosan gels with thymol using supercritical carbon dioxide (scCO2) was studied in order to obtain new materials with added value without the use of organic solvents. The optimum conditions of supercritical impregnation (15 MPa, 35 °C and 24 h) were defined based on previously investigated thymol solubility in scCO2. Cross-linkers formaldehyde and glutardialdehyde were used to prepare chitosan hydrogels. The obtained hydrogels of chitosan were converted in acetogels and then dried with air or by using scCO2 in order to obtain xerogels and aerogels. The best results, in terms of morphology and yield of impregnation with thymol, have been achieved for the aerogel of chitosan (11.30%) which was obtained with cross-linker formaldehyde and dried with scCO2 at 15 MPa and 50 °C., U radu je ispitivana impregnacija različito pripremljenih hitozan gelova sa timolom pomoću natkritičnog ugljenik(IV)-oksida (nkCO2) u cilju dobijanja novih materijala sa dodatom vrednošću bez upotrebe organskih rastvarača. Optimalni uslovi natkritične impregnacije (15 MPa, 35 °C i 24 h) su definisani na osnovu prethodno ispitane rastvorljivosti timola u nkCO2. Za pripremu hidrogelova hitozana korišćeni su umreživači formaldehid i glutardialdehid. Dobijeni hidrogelovi hitozana su prevedeni u acetogelove, a potom sušeni na vazduhu ili pomoću nkCO2 kako bi se dobili kserogelovi ili aerogelovi. Najbolji rezultati u pogledu morfologije i prinosa impregancije sa timolom postignuti su za aerogel hitozana (11,30%) koji je dobijen umrežavanjem sa formaldehidom i sušenjem sa nkCO2 na 15 MPa i 50 °C.",
publisher = "Savez inženjera i tehničara Srbije, Beograd",
journal = "Tehnika",
title = "Optimization of chitosan gel preparation for supercritical impregnation of thymol, Uticaj pripreme hitozan gelova na natkritičnu impregnaciju sa timolom",
pages = "396-391",
number = "3",
volume = "70",
doi = "10.5937/tehnika1503391M"
}

Milovanović, S., Pantić, M. M., Ivanović, J.,& Žižović, I.. (2015). Optimization of chitosan gel preparation for supercritical impregnation of thymol. in Tehnika
Savez inženjera i tehničara Srbije, Beograd., 70(3), 391-396.
https://doi.org/10.5937/tehnika1503391M

Milovanović S, Pantić MM, Ivanović J, Žižović I. Optimization of chitosan gel preparation for supercritical impregnation of thymol. in Tehnika. 2015;70(3):391-396.
doi:10.5937/tehnika1503391M .

Milovanović, Stoja, Pantić, Milica M., Ivanović, Jasna, Žižović, Irena, "Optimization of chitosan gel preparation for supercritical impregnation of thymol" in Tehnika, 70, no. 3 (2015):391-396,
https://doi.org/10.5937/tehnika1503391M . .