TY  - CONF
AU  - Gruden-Movsesijan, Alisa
AU  - Tomić, Sergej
AU  - Ilić, Nataša
AU  - Đokić, Jelena
AU  - Glamočlija, Sofija
AU  - Vasilev, Saša
AU  - Sabljić, Ljiljana
AU  - Stojanović, Dušica
AU  - Miljković, Đorđe
AU  - Dinić, Miroslav
AU  - Radojević, Dušan
AU  - Jevtić, Bojan
AU  - Golić, Nataša
AU  - Uskoković, Petar
AU  - Sofronić-Milosavljević, Ljiljana
AU  - Čolić, Miodrag
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6671
AB  - Trichinella spiralis possess the potential to induce tolerance and restrain excessive immune responses, thus
exerting beneficial effect on the outcome of experimental autoimmune encephalomyelitis (EAE), the animal
model of human disease multiple sclerosis. Trichinella achieves this by continuous release of its excretoysecretory (ES L1) products in the form of a complex mixture of individual components and extracellular vesicles
(TsEVs), preferentially exsosomes. Our previous results suggested that the complex modulation of the host
immune response is achieved by ES L1 products or its individula components, which induce the maturation
of dendritic cells towards tolerogenic status. A new delivery system, based on nanomaterials as carriers of ES
L1, was designed to resemble what happens in nature, ie. spontaneous release of Trichinella products over
an extended period of time using a less invasive route of administration than all previously described for the
treatment of autoimmune diseases. PLGA biodegradable nanofibers loaded with ES L1 (PLGA-ES L1), were
used as subcutaneous implants. The tretment proved to be safe with no side effects, successful in delivering
parasite products and mitigating the course of relapsing/remitting EAE in Dark Agouti rats. The mechanisms
we examined included events at the systemic level and target tissue, spinal cord, that explain the shift from the
pro- to anti-inflammatory responses and success of treatment. PLGA-ES L1 treatment also prevents the EAEinduced disruption of intestinal epithelial barrier. It diminished the inflammation by lowering the expression
of TNF-α in gastrointestinal tract (GIT) and succesfully maintain the expression of mRNA for claudin, the most
important tight junction protein in GIT. Obseved protective effect was acompained with the re-establishment of gut microbiota diversity that was disturbed in EAE-induced animals.
PB  - Belgrade : Serbian Society for Parasitology
C3  - Programme & Abstract Book / 13th European Multicolloquium of Parasitology Belgrade, October 12-16
T1  - New delivery system for Trichinella spiralis antigens - accelerated approach to autoimmunity treatment
SP  - 92
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6671
ER  - 

@conference{
author = "Gruden-Movsesijan, Alisa and Tomić, Sergej and Ilić, Nataša and Đokić, Jelena and Glamočlija, Sofija and Vasilev, Saša and Sabljić, Ljiljana and Stojanović, Dušica and Miljković, Đorđe and Dinić, Miroslav and Radojević, Dušan and Jevtić, Bojan and Golić, Nataša and Uskoković, Petar and Sofronić-Milosavljević, Ljiljana and Čolić, Miodrag",
year = "2021",
abstract = "Trichinella spiralis possess the potential to induce tolerance and restrain excessive immune responses, thus
exerting beneficial effect on the outcome of experimental autoimmune encephalomyelitis (EAE), the animal
model of human disease multiple sclerosis. Trichinella achieves this by continuous release of its excretoysecretory (ES L1) products in the form of a complex mixture of individual components and extracellular vesicles
(TsEVs), preferentially exsosomes. Our previous results suggested that the complex modulation of the host
immune response is achieved by ES L1 products or its individula components, which induce the maturation
of dendritic cells towards tolerogenic status. A new delivery system, based on nanomaterials as carriers of ES
L1, was designed to resemble what happens in nature, ie. spontaneous release of Trichinella products over
an extended period of time using a less invasive route of administration than all previously described for the
treatment of autoimmune diseases. PLGA biodegradable nanofibers loaded with ES L1 (PLGA-ES L1), were
used as subcutaneous implants. The tretment proved to be safe with no side effects, successful in delivering
parasite products and mitigating the course of relapsing/remitting EAE in Dark Agouti rats. The mechanisms
we examined included events at the systemic level and target tissue, spinal cord, that explain the shift from the
pro- to anti-inflammatory responses and success of treatment. PLGA-ES L1 treatment also prevents the EAEinduced disruption of intestinal epithelial barrier. It diminished the inflammation by lowering the expression
of TNF-α in gastrointestinal tract (GIT) and succesfully maintain the expression of mRNA for claudin, the most
important tight junction protein in GIT. Obseved protective effect was acompained with the re-establishment of gut microbiota diversity that was disturbed in EAE-induced animals.",
publisher = "Belgrade : Serbian Society for Parasitology",
journal = "Programme & Abstract Book / 13th European Multicolloquium of Parasitology Belgrade, October 12-16",
title = "New delivery system for Trichinella spiralis antigens - accelerated approach to autoimmunity treatment",
pages = "92",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6671"
}

Gruden-Movsesijan, A., Tomić, S., Ilić, N., Đokić, J., Glamočlija, S., Vasilev, S., Sabljić, L., Stojanović, D., Miljković, Đ., Dinić, M., Radojević, D., Jevtić, B., Golić, N., Uskoković, P., Sofronić-Milosavljević, L.,& Čolić, M.. (2021). New delivery system for Trichinella spiralis antigens - accelerated approach to autoimmunity treatment. in Programme & Abstract Book / 13th European Multicolloquium of Parasitology Belgrade, October 12-16
Belgrade : Serbian Society for Parasitology., 92.
https://hdl.handle.net/21.15107/rcub_technorep_6671

Gruden-Movsesijan A, Tomić S, Ilić N, Đokić J, Glamočlija S, Vasilev S, Sabljić L, Stojanović D, Miljković Đ, Dinić M, Radojević D, Jevtić B, Golić N, Uskoković P, Sofronić-Milosavljević L, Čolić M. New delivery system for Trichinella spiralis antigens - accelerated approach to autoimmunity treatment. in Programme & Abstract Book / 13th European Multicolloquium of Parasitology Belgrade, October 12-16. 2021;:92.
https://hdl.handle.net/21.15107/rcub_technorep_6671 .

Gruden-Movsesijan, Alisa, Tomić, Sergej, Ilić, Nataša, Đokić, Jelena, Glamočlija, Sofija, Vasilev, Saša, Sabljić, Ljiljana, Stojanović, Dušica, Miljković, Đorđe, Dinić, Miroslav, Radojević, Dušan, Jevtić, Bojan, Golić, Nataša, Uskoković, Petar, Sofronić-Milosavljević, Ljiljana, Čolić, Miodrag, "New delivery system for Trichinella spiralis antigens - accelerated approach to autoimmunity treatment" in Programme & Abstract Book / 13th European Multicolloquium of Parasitology Belgrade, October 12-16 (2021):92,
https://hdl.handle.net/21.15107/rcub_technorep_6671 .

TY  - JOUR
AU  - Čolić, Miodrag
AU  - Dzopalić, Tanja
AU  - Tomić, Sergej
AU  - Rajković, Jelena
AU  - Rudolf, Rebeka
AU  - Vuković, Goran D.
AU  - Marinković, Aleksandar
AU  - Uskoković, Petar
PY  - 2014
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2782
AB  - The protocols for the preparation of dendritic cells (DCs) as cancer vaccines based on stimulation of Toll-like receptors (TLRs) are very promising. In this study we covalently attached 7-thia-8-oxoguanosine (7-TOG), a selective endosomal TLR7, to previously oxidized multi-walled carbon nanotubes (o-MWCNTs) (mass ratio 1:5.6, respectively), and tested their ability to activate human monocyte-derived (Mo)DCs. Light, confocal and transmission electron microscopy confirmed efficient phagocytosis of 7-TOG-MWCNTs by MoDCs and their efficient delivery to TLR7(+) endosomes. The biocompatibility studies showed that 7-TOG-MWCNTs, at concentrations lower than 100 mu g/ml, were not cytotoxic for MoDCs. 7-TOG-MV/CNTs (50 mu g/ml) up-regulated CD86 expression, allostimulatory activity, T helper (Th)1- and Th17-polarizing capability of MoDCs. The same concentration of soluble 7-TOG, alone or in combination with control o-MWCNTs, did not have such effects. It can be hypothesized that the efficacy of 7-TOG-MWCNTs in stimulating MoDCs was a consequence of increased intracellular concentration of 7-TOG after internalization of the nano-complexes, because similar Th-polarizing capability could be induced with 10-times higher concentrations of soluble 7-TOG. In conclusion, our results suggest that functionalized MWCNTs may be a promising system for the delivery of drugs to intracellular targets, in order to improve the immunogenic potential of DCs for therapeutic purposes.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Carbon
T1  - Immunomodulatory effects of carbon nanotubes functionalized with a Toll-like receptor 7 agonist on human dendritic cells
EP  - 287
SP  - 273
VL  - 67
DO  - 10.1016/j.carbon.2013.09.090
ER  - 

@article{
author = "Čolić, Miodrag and Dzopalić, Tanja and Tomić, Sergej and Rajković, Jelena and Rudolf, Rebeka and Vuković, Goran D. and Marinković, Aleksandar and Uskoković, Petar",
year = "2014",
abstract = "The protocols for the preparation of dendritic cells (DCs) as cancer vaccines based on stimulation of Toll-like receptors (TLRs) are very promising. In this study we covalently attached 7-thia-8-oxoguanosine (7-TOG), a selective endosomal TLR7, to previously oxidized multi-walled carbon nanotubes (o-MWCNTs) (mass ratio 1:5.6, respectively), and tested their ability to activate human monocyte-derived (Mo)DCs. Light, confocal and transmission electron microscopy confirmed efficient phagocytosis of 7-TOG-MWCNTs by MoDCs and their efficient delivery to TLR7(+) endosomes. The biocompatibility studies showed that 7-TOG-MWCNTs, at concentrations lower than 100 mu g/ml, were not cytotoxic for MoDCs. 7-TOG-MV/CNTs (50 mu g/ml) up-regulated CD86 expression, allostimulatory activity, T helper (Th)1- and Th17-polarizing capability of MoDCs. The same concentration of soluble 7-TOG, alone or in combination with control o-MWCNTs, did not have such effects. It can be hypothesized that the efficacy of 7-TOG-MWCNTs in stimulating MoDCs was a consequence of increased intracellular concentration of 7-TOG after internalization of the nano-complexes, because similar Th-polarizing capability could be induced with 10-times higher concentrations of soluble 7-TOG. In conclusion, our results suggest that functionalized MWCNTs may be a promising system for the delivery of drugs to intracellular targets, in order to improve the immunogenic potential of DCs for therapeutic purposes.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Carbon",
title = "Immunomodulatory effects of carbon nanotubes functionalized with a Toll-like receptor 7 agonist on human dendritic cells",
pages = "287-273",
volume = "67",
doi = "10.1016/j.carbon.2013.09.090"
}

Čolić, M., Dzopalić, T., Tomić, S., Rajković, J., Rudolf, R., Vuković, G. D., Marinković, A.,& Uskoković, P.. (2014). Immunomodulatory effects of carbon nanotubes functionalized with a Toll-like receptor 7 agonist on human dendritic cells. in Carbon
Pergamon-Elsevier Science Ltd, Oxford., 67, 273-287.
https://doi.org/10.1016/j.carbon.2013.09.090

Čolić M, Dzopalić T, Tomić S, Rajković J, Rudolf R, Vuković GD, Marinković A, Uskoković P. Immunomodulatory effects of carbon nanotubes functionalized with a Toll-like receptor 7 agonist on human dendritic cells. in Carbon. 2014;67:273-287.
doi:10.1016/j.carbon.2013.09.090 .

Čolić, Miodrag, Dzopalić, Tanja, Tomić, Sergej, Rajković, Jelena, Rudolf, Rebeka, Vuković, Goran D., Marinković, Aleksandar, Uskoković, Petar, "Immunomodulatory effects of carbon nanotubes functionalized with a Toll-like receptor 7 agonist on human dendritic cells" in Carbon, 67 (2014):273-287,
https://doi.org/10.1016/j.carbon.2013.09.090 . .

TY  - JOUR
AU  - Veljović, Đorđe
AU  - Čolić, Miodrag
AU  - Kojić, Vesna
AU  - Bogdanović, Gordana
AU  - Kojić, Zvezdana
AU  - Banjac, Andrijana
AU  - Palcevskis, Eriks
AU  - Petrović, Rada
AU  - Janaćković, Đorđe
PY  - 2012
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2174
AB  - The effect of decreasing the grain size on the biocompatibility, cellmaterial interface, and mechanical properties of microwave-sintered monophase hydroxyapatite bioceramics was investigated in this study. A nanosized stoichiometric hydroxyapatite powder was isostatically pressed at high pressure and sintered in a microwave furnace in order to obtain fine grained dense bioceramics. The samples sintered at 1200 degrees C, with a density near the theoretical one, were composed of micron-sized grains, while the grain size decreased to 130 nm on decreasing the sintering temperature to 900 degrees C. This decrease in the grain size certainly led to increases in the fracture toughness by much as 54%. An in vitro investigation of biocompatibility with L929 and human MRC-5 fibroblast cells showed noncytotoxic effects for both types of bioceramics, while the relative cell proliferation rate, cell attachment and metabolic activity of the fibroblasts were improved with decreasing of grain size. An initial in vivo investigation of biocompatibility by the primary cutaneous irritation test showed that both materials exhibited no irritation properties.
PB  - Wiley, Hoboken
T2  - Journal of Biomedical Materials Research Part A
T1  - The effect of grain size on the biocompatibility, cell-materials interface, and mechanical properties of microwave-sintered bioceramics
EP  - 3070
IS  - 11
SP  - 3059
VL  - 100A
DO  - 10.1002/jbm.a.34225
ER  - 

@article{
author = "Veljović, Đorđe and Čolić, Miodrag and Kojić, Vesna and Bogdanović, Gordana and Kojić, Zvezdana and Banjac, Andrijana and Palcevskis, Eriks and Petrović, Rada and Janaćković, Đorđe",
year = "2012",
abstract = "The effect of decreasing the grain size on the biocompatibility, cellmaterial interface, and mechanical properties of microwave-sintered monophase hydroxyapatite bioceramics was investigated in this study. A nanosized stoichiometric hydroxyapatite powder was isostatically pressed at high pressure and sintered in a microwave furnace in order to obtain fine grained dense bioceramics. The samples sintered at 1200 degrees C, with a density near the theoretical one, were composed of micron-sized grains, while the grain size decreased to 130 nm on decreasing the sintering temperature to 900 degrees C. This decrease in the grain size certainly led to increases in the fracture toughness by much as 54%. An in vitro investigation of biocompatibility with L929 and human MRC-5 fibroblast cells showed noncytotoxic effects for both types of bioceramics, while the relative cell proliferation rate, cell attachment and metabolic activity of the fibroblasts were improved with decreasing of grain size. An initial in vivo investigation of biocompatibility by the primary cutaneous irritation test showed that both materials exhibited no irritation properties.",
publisher = "Wiley, Hoboken",
journal = "Journal of Biomedical Materials Research Part A",
title = "The effect of grain size on the biocompatibility, cell-materials interface, and mechanical properties of microwave-sintered bioceramics",
pages = "3070-3059",
number = "11",
volume = "100A",
doi = "10.1002/jbm.a.34225"
}

Veljović, Đ., Čolić, M., Kojić, V., Bogdanović, G., Kojić, Z., Banjac, A., Palcevskis, E., Petrović, R.,& Janaćković, Đ.. (2012). The effect of grain size on the biocompatibility, cell-materials interface, and mechanical properties of microwave-sintered bioceramics. in Journal of Biomedical Materials Research Part A
Wiley, Hoboken., 100A(11), 3059-3070.
https://doi.org/10.1002/jbm.a.34225

Veljović Đ, Čolić M, Kojić V, Bogdanović G, Kojić Z, Banjac A, Palcevskis E, Petrović R, Janaćković Đ. The effect of grain size on the biocompatibility, cell-materials interface, and mechanical properties of microwave-sintered bioceramics. in Journal of Biomedical Materials Research Part A. 2012;100A(11):3059-3070.
doi:10.1002/jbm.a.34225 .

Veljović, Đorđe, Čolić, Miodrag, Kojić, Vesna, Bogdanović, Gordana, Kojić, Zvezdana, Banjac, Andrijana, Palcevskis, Eriks, Petrović, Rada, Janaćković, Đorđe, "The effect of grain size on the biocompatibility, cell-materials interface, and mechanical properties of microwave-sintered bioceramics" in Journal of Biomedical Materials Research Part A, 100A, no. 11 (2012):3059-3070,
https://doi.org/10.1002/jbm.a.34225 . .

TY  - JOUR
AU  - Vuković, Goran D.
AU  - Marinković, Aleksandar
AU  - Čolić, Miodrag
AU  - Ristić, Mirjana
AU  - Aleksić, Radoslav
AU  - Perić-Grujić, Aleksandra
AU  - Uskoković, Petar
PY  - 2010
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1714
AB  - Surface functionalization of multi-walled carbon nanotubes (MWCNTs) by ethylenediamine, via chemical modification of carboxyl groups, using O-(7-azabenzotriazol-1-yl)-N,N,N',N'-tetramethyluronium hexafluorophosphate, was performed. The resulting materials were characterized by different techniques, such as FTIR, TGA and elemental analysis. Biocompatibility studies showed that the functionalized MWCNTs, at concentrations between 1 and 50 mu g mL(-1), were not cytotoxic for the fibroblast L929 cell line. In batch tests, the influences of solution pH, contact time, initial metal ion concentration and temperature on the sorption of Cd2+ ions onto raw-MWCNTs (raw-MWCNT), oxidized MWCNTs (o-MWCNT) and ethylenediamine-functionalized MWCNTs (e-MWCNT) were studied. The adsorption of Cd2+ ions by o-MWCNT and e-MWCNT was strongly pH dependent. The time dependent Cd2+ sorption onto raw-MWCNT, o-MWCNT and e-MWCNT can be described by a pseudo-second-order kinetic model. The Langmuir isotherm model agrees well with the equilibrium experimental data. The maximum capacity was obtained for e-MWCNT, 25.7 mgg(-1), at 45 degrees C. The thermodynamic parameters were also deduced for the adsorption of Cd2+ ions on raw-MWCNT, o-MWCNT and e-MWCNT and the results showed that the adsorption was spontaneous and endothermic.
PB  - Elsevier Science Sa, Lausanne
T2  - Chemical Engineering Journal
T1  - Removal of cadmium from aqueous solutions by oxidized and ethylenediamine-functionalized multi-walled carbon nanotubes
EP  - 248
IS  - 1
SP  - 238
VL  - 157
DO  - 10.1016/j.cej.2009.11.026
ER  - 

@article{
author = "Vuković, Goran D. and Marinković, Aleksandar and Čolić, Miodrag and Ristić, Mirjana and Aleksić, Radoslav and Perić-Grujić, Aleksandra and Uskoković, Petar",
year = "2010",
abstract = "Surface functionalization of multi-walled carbon nanotubes (MWCNTs) by ethylenediamine, via chemical modification of carboxyl groups, using O-(7-azabenzotriazol-1-yl)-N,N,N',N'-tetramethyluronium hexafluorophosphate, was performed. The resulting materials were characterized by different techniques, such as FTIR, TGA and elemental analysis. Biocompatibility studies showed that the functionalized MWCNTs, at concentrations between 1 and 50 mu g mL(-1), were not cytotoxic for the fibroblast L929 cell line. In batch tests, the influences of solution pH, contact time, initial metal ion concentration and temperature on the sorption of Cd2+ ions onto raw-MWCNTs (raw-MWCNT), oxidized MWCNTs (o-MWCNT) and ethylenediamine-functionalized MWCNTs (e-MWCNT) were studied. The adsorption of Cd2+ ions by o-MWCNT and e-MWCNT was strongly pH dependent. The time dependent Cd2+ sorption onto raw-MWCNT, o-MWCNT and e-MWCNT can be described by a pseudo-second-order kinetic model. The Langmuir isotherm model agrees well with the equilibrium experimental data. The maximum capacity was obtained for e-MWCNT, 25.7 mgg(-1), at 45 degrees C. The thermodynamic parameters were also deduced for the adsorption of Cd2+ ions on raw-MWCNT, o-MWCNT and e-MWCNT and the results showed that the adsorption was spontaneous and endothermic.",
publisher = "Elsevier Science Sa, Lausanne",
journal = "Chemical Engineering Journal",
title = "Removal of cadmium from aqueous solutions by oxidized and ethylenediamine-functionalized multi-walled carbon nanotubes",
pages = "248-238",
number = "1",
volume = "157",
doi = "10.1016/j.cej.2009.11.026"
}

Vuković, G. D., Marinković, A., Čolić, M., Ristić, M., Aleksić, R., Perić-Grujić, A.,& Uskoković, P.. (2010). Removal of cadmium from aqueous solutions by oxidized and ethylenediamine-functionalized multi-walled carbon nanotubes. in Chemical Engineering Journal
Elsevier Science Sa, Lausanne., 157(1), 238-248.
https://doi.org/10.1016/j.cej.2009.11.026

Vuković GD, Marinković A, Čolić M, Ristić M, Aleksić R, Perić-Grujić A, Uskoković P. Removal of cadmium from aqueous solutions by oxidized and ethylenediamine-functionalized multi-walled carbon nanotubes. in Chemical Engineering Journal. 2010;157(1):238-248.
doi:10.1016/j.cej.2009.11.026 .

Vuković, Goran D., Marinković, Aleksandar, Čolić, Miodrag, Ristić, Mirjana, Aleksić, Radoslav, Perić-Grujić, Aleksandra, Uskoković, Petar, "Removal of cadmium from aqueous solutions by oxidized and ethylenediamine-functionalized multi-walled carbon nanotubes" in Chemical Engineering Journal, 157, no. 1 (2010):238-248,
https://doi.org/10.1016/j.cej.2009.11.026 . .

TY  - JOUR
AU  - Vuković, Goran D.
AU  - Tomić, Sergej
AU  - Marinković, Aleksandar
AU  - Radmilović, Velimir R.
AU  - Uskoković, Petar
AU  - Čolić, Miodrag
PY  - 2010
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1648
AB  - Dapsone is an anti-microbial and anti-inflammatory drug. Water-dispersible dapsone-modified multi-wall carbon nanotubes (dap-MWCNTs) were prepared by chemical modification of the carboxyl groups introduced on the surface of the nanotubes using O-(7-aza-benzotriazol-1-yl)-N,N,N',N'-tetramethyluronium hexafluorophosphate (N-HATU) and N,N-diisopropylethylamine (DIEA). The modification was confirmed by Fourier-transform infrared spectroscopy, transmission election microscopy and thermogravimetric analysis. The biological effect of dap-MWCNTs was tested using rat peritoneal macrophages (PMempty set). By confocal laser microscopy and flow cytometry, it was shown that the dap-MWCNTs were rapidly ingested by PMempty set as were the control, oxidized o-MWCNTs. Neither dap-MWCNTs at lower concentrations (up to 50 mu g/ml), nor o-MWCNTs, at equivalent concentrations, respectively affected the viability of PMempty set, while higher concentrations triggered apoptosis. Apoptosis of PMempty set induced by the control, o-MWCNTs, was higher than that induced by dap-MWCNTs and it correlated with the induction of oxidative stress. In contrast, dap-MWCNTs did not trigger oxidative stress but caused apoptosis of PMempty set predominantly after prolonged cultivation (3 days). Although equivalent concentrations of soluble dapsone induced oxidative stress, they were anti-apoptotic. Cumulatively, the obtained results show the complexity of dap-MWCNT/PMempty set interactions and suggest that this complex could be investigated for the treatment of dapsone-sensitive intracellular microorganisms or inflammatory diseases responding to dapsone therapy.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Carbon
T1  - The response of peritoneal macrophages to dapsone covalently attached on the surface of carbon nanotubes
EP  - 3078
IS  - 11
SP  - 3066
VL  - 48
DO  - 10.1016/j.carbon.2010.04.043
ER  - 

@article{
author = "Vuković, Goran D. and Tomić, Sergej and Marinković, Aleksandar and Radmilović, Velimir R. and Uskoković, Petar and Čolić, Miodrag",
year = "2010",
abstract = "Dapsone is an anti-microbial and anti-inflammatory drug. Water-dispersible dapsone-modified multi-wall carbon nanotubes (dap-MWCNTs) were prepared by chemical modification of the carboxyl groups introduced on the surface of the nanotubes using O-(7-aza-benzotriazol-1-yl)-N,N,N',N'-tetramethyluronium hexafluorophosphate (N-HATU) and N,N-diisopropylethylamine (DIEA). The modification was confirmed by Fourier-transform infrared spectroscopy, transmission election microscopy and thermogravimetric analysis. The biological effect of dap-MWCNTs was tested using rat peritoneal macrophages (PMempty set). By confocal laser microscopy and flow cytometry, it was shown that the dap-MWCNTs were rapidly ingested by PMempty set as were the control, oxidized o-MWCNTs. Neither dap-MWCNTs at lower concentrations (up to 50 mu g/ml), nor o-MWCNTs, at equivalent concentrations, respectively affected the viability of PMempty set, while higher concentrations triggered apoptosis. Apoptosis of PMempty set induced by the control, o-MWCNTs, was higher than that induced by dap-MWCNTs and it correlated with the induction of oxidative stress. In contrast, dap-MWCNTs did not trigger oxidative stress but caused apoptosis of PMempty set predominantly after prolonged cultivation (3 days). Although equivalent concentrations of soluble dapsone induced oxidative stress, they were anti-apoptotic. Cumulatively, the obtained results show the complexity of dap-MWCNT/PMempty set interactions and suggest that this complex could be investigated for the treatment of dapsone-sensitive intracellular microorganisms or inflammatory diseases responding to dapsone therapy.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Carbon",
title = "The response of peritoneal macrophages to dapsone covalently attached on the surface of carbon nanotubes",
pages = "3078-3066",
number = "11",
volume = "48",
doi = "10.1016/j.carbon.2010.04.043"
}

Vuković, G. D., Tomić, S., Marinković, A., Radmilović, V. R., Uskoković, P.,& Čolić, M.. (2010). The response of peritoneal macrophages to dapsone covalently attached on the surface of carbon nanotubes. in Carbon
Pergamon-Elsevier Science Ltd, Oxford., 48(11), 3066-3078.
https://doi.org/10.1016/j.carbon.2010.04.043

Vuković GD, Tomić S, Marinković A, Radmilović VR, Uskoković P, Čolić M. The response of peritoneal macrophages to dapsone covalently attached on the surface of carbon nanotubes. in Carbon. 2010;48(11):3066-3078.
doi:10.1016/j.carbon.2010.04.043 .

Vuković, Goran D., Tomić, Sergej, Marinković, Aleksandar, Radmilović, Velimir R., Uskoković, Petar, Čolić, Miodrag, "The response of peritoneal macrophages to dapsone covalently attached on the surface of carbon nanotubes" in Carbon, 48, no. 11 (2010):3066-3078,
https://doi.org/10.1016/j.carbon.2010.04.043 . .

TY  - JOUR
AU  - Vuković, Goran D.
AU  - Marinković, Aleksandar
AU  - Obradović, Maja
AU  - Radmilović, V.
AU  - Čolić, Miodrag
AU  - Aleksić, Radoslav
AU  - Uskoković, Petar
PY  - 2009
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1512
AB  - Surface functionalization of multi-walled carbon nanotubes (MWCNTs), with amino groups via chemical modification of carboxyl groups introduced on the nanotube surface, using O-(7-azabenzotriazol-1-yl)-N, N,N',N'-tetramethyluronium hexafluorophosphate (N-HATU) and N,N-diisopropylethylamine (DIEA) is reported. The N-HATU coupling agent provides faster reaction rate and the reaction occurs at lower temperature compared to amidation and acylation-amidation chemistry. The amines, 1,6-hexanediamine (HDA), diethylenetriamine (DETA), triethylenetetramine (TETA) and 1,4-phenylenediamine (PDA) were used. The resulting materials were characterized with different techniques such as FTIR, XRD, elemental analysis, TGA, TEM, UV-vis spectroscopy and cyclic voltammetry. MWCNTs functionalized with PDA posses the best dispersibility and electron transfer properties in comparison to the others amines. Functionalized MWCNTs, at the concentrations between 1 and 50 mu g ml(-1), were not cytotoxic for the fibroblast L929 cell line. However, the concentrations of MWCNTs higher of 10 mu g ml(-1) reduced cell growth and this effect correlated positively with the degree of their uptake by L929 cells.
PB  - Elsevier Science Bv, Amsterdam
T2  - Applied Surface Science
T1  - Synthesis, characterization and cytotoxicity of surface amino-functionalized water-dispersible multi-walled carbon nanotubes
EP  - 8075
IS  - 18
SP  - 8067
VL  - 255
DO  - 10.1016/j.apsusc.2009.05.016
ER  - 

@article{
author = "Vuković, Goran D. and Marinković, Aleksandar and Obradović, Maja and Radmilović, V. and Čolić, Miodrag and Aleksić, Radoslav and Uskoković, Petar",
year = "2009",
abstract = "Surface functionalization of multi-walled carbon nanotubes (MWCNTs), with amino groups via chemical modification of carboxyl groups introduced on the nanotube surface, using O-(7-azabenzotriazol-1-yl)-N, N,N',N'-tetramethyluronium hexafluorophosphate (N-HATU) and N,N-diisopropylethylamine (DIEA) is reported. The N-HATU coupling agent provides faster reaction rate and the reaction occurs at lower temperature compared to amidation and acylation-amidation chemistry. The amines, 1,6-hexanediamine (HDA), diethylenetriamine (DETA), triethylenetetramine (TETA) and 1,4-phenylenediamine (PDA) were used. The resulting materials were characterized with different techniques such as FTIR, XRD, elemental analysis, TGA, TEM, UV-vis spectroscopy and cyclic voltammetry. MWCNTs functionalized with PDA posses the best dispersibility and electron transfer properties in comparison to the others amines. Functionalized MWCNTs, at the concentrations between 1 and 50 mu g ml(-1), were not cytotoxic for the fibroblast L929 cell line. However, the concentrations of MWCNTs higher of 10 mu g ml(-1) reduced cell growth and this effect correlated positively with the degree of their uptake by L929 cells.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Applied Surface Science",
title = "Synthesis, characterization and cytotoxicity of surface amino-functionalized water-dispersible multi-walled carbon nanotubes",
pages = "8075-8067",
number = "18",
volume = "255",
doi = "10.1016/j.apsusc.2009.05.016"
}

Vuković, G. D., Marinković, A., Obradović, M., Radmilović, V., Čolić, M., Aleksić, R.,& Uskoković, P.. (2009). Synthesis, characterization and cytotoxicity of surface amino-functionalized water-dispersible multi-walled carbon nanotubes. in Applied Surface Science
Elsevier Science Bv, Amsterdam., 255(18), 8067-8075.
https://doi.org/10.1016/j.apsusc.2009.05.016

Vuković GD, Marinković A, Obradović M, Radmilović V, Čolić M, Aleksić R, Uskoković P. Synthesis, characterization and cytotoxicity of surface amino-functionalized water-dispersible multi-walled carbon nanotubes. in Applied Surface Science. 2009;255(18):8067-8075.
doi:10.1016/j.apsusc.2009.05.016 .

Vuković, Goran D., Marinković, Aleksandar, Obradović, Maja, Radmilović, V., Čolić, Miodrag, Aleksić, Radoslav, Uskoković, Petar, "Synthesis, characterization and cytotoxicity of surface amino-functionalized water-dispersible multi-walled carbon nanotubes" in Applied Surface Science, 255, no. 18 (2009):8067-8075,
https://doi.org/10.1016/j.apsusc.2009.05.016 . .