New delivery system for Trichinella spiralis antigens - accelerated approach to autoimmunity treatment
Само за регистроване кориснике
2021
Аутори
Gruden-Movsesijan, AlisaTomić, Sergej
Ilić, Nataša
Đokić, Jelena
Glamočlija, Sofija
Vasilev, Saša
Sabljić, Ljiljana
Stojanović, Dušica
Miljković, Đorđe
Dinić, Miroslav
Radojević, Dušan
Jevtić, Bojan
Golić, Nataša
Uskoković, Petar
Sofronić-Milosavljević, Ljiljana
Čolić, Miodrag
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Trichinella spiralis possess the potential to induce tolerance and restrain excessive immune responses, thus
exerting beneficial effect on the outcome of experimental autoimmune encephalomyelitis (EAE), the animal
model of human disease multiple sclerosis. Trichinella achieves this by continuous release of its excretoysecretory (ES L1) products in the form of a complex mixture of individual components and extracellular vesicles
(TsEVs), preferentially exsosomes. Our previous results suggested that the complex modulation of the host
immune response is achieved by ES L1 products or its individula components, which induce the maturation
of dendritic cells towards tolerogenic status. A new delivery system, based on nanomaterials as carriers of ES
L1, was designed to resemble what happens in nature, ie. spontaneous release of Trichinella products over
an extended period of time using a less invasive route of administration than all previously described for the
treatment of autoimmun...e diseases. PLGA biodegradable nanofibers loaded with ES L1 (PLGA-ES L1), were
used as subcutaneous implants. The tretment proved to be safe with no side effects, successful in delivering
parasite products and mitigating the course of relapsing/remitting EAE in Dark Agouti rats. The mechanisms
we examined included events at the systemic level and target tissue, spinal cord, that explain the shift from the
pro- to anti-inflammatory responses and success of treatment. PLGA-ES L1 treatment also prevents the EAEinduced disruption of intestinal epithelial barrier. It diminished the inflammation by lowering the expression
of TNF-α in gastrointestinal tract (GIT) and succesfully maintain the expression of mRNA for claudin, the most
important tight junction protein in GIT. Obseved protective effect was acompained with the re-establishment of gut microbiota diversity that was disturbed in EAE-induced animals.
Извор:
Programme & Abstract Book / 13th European Multicolloquium of Parasitology Belgrade, October 12-16, 2021, 92-Издавач:
- Belgrade : Serbian Society for Parasitology
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200019 (Универзитет у Београду, Институт за примену нуклеарне енергије - ИНЕП) (RS-MESTD-inst-2020-200019)
Институција/група
Tehnološko-metalurški fakultetTY - CONF AU - Gruden-Movsesijan, Alisa AU - Tomić, Sergej AU - Ilić, Nataša AU - Đokić, Jelena AU - Glamočlija, Sofija AU - Vasilev, Saša AU - Sabljić, Ljiljana AU - Stojanović, Dušica AU - Miljković, Đorđe AU - Dinić, Miroslav AU - Radojević, Dušan AU - Jevtić, Bojan AU - Golić, Nataša AU - Uskoković, Petar AU - Sofronić-Milosavljević, Ljiljana AU - Čolić, Miodrag PY - 2021 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6671 AB - Trichinella spiralis possess the potential to induce tolerance and restrain excessive immune responses, thus exerting beneficial effect on the outcome of experimental autoimmune encephalomyelitis (EAE), the animal model of human disease multiple sclerosis. Trichinella achieves this by continuous release of its excretoysecretory (ES L1) products in the form of a complex mixture of individual components and extracellular vesicles (TsEVs), preferentially exsosomes. Our previous results suggested that the complex modulation of the host immune response is achieved by ES L1 products or its individula components, which induce the maturation of dendritic cells towards tolerogenic status. A new delivery system, based on nanomaterials as carriers of ES L1, was designed to resemble what happens in nature, ie. spontaneous release of Trichinella products over an extended period of time using a less invasive route of administration than all previously described for the treatment of autoimmune diseases. PLGA biodegradable nanofibers loaded with ES L1 (PLGA-ES L1), were used as subcutaneous implants. The tretment proved to be safe with no side effects, successful in delivering parasite products and mitigating the course of relapsing/remitting EAE in Dark Agouti rats. The mechanisms we examined included events at the systemic level and target tissue, spinal cord, that explain the shift from the pro- to anti-inflammatory responses and success of treatment. PLGA-ES L1 treatment also prevents the EAEinduced disruption of intestinal epithelial barrier. It diminished the inflammation by lowering the expression of TNF-α in gastrointestinal tract (GIT) and succesfully maintain the expression of mRNA for claudin, the most important tight junction protein in GIT. Obseved protective effect was acompained with the re-establishment of gut microbiota diversity that was disturbed in EAE-induced animals. PB - Belgrade : Serbian Society for Parasitology C3 - Programme & Abstract Book / 13th European Multicolloquium of Parasitology Belgrade, October 12-16 T1 - New delivery system for Trichinella spiralis antigens - accelerated approach to autoimmunity treatment SP - 92 UR - https://hdl.handle.net/21.15107/rcub_technorep_6671 ER -
@conference{ author = "Gruden-Movsesijan, Alisa and Tomić, Sergej and Ilić, Nataša and Đokić, Jelena and Glamočlija, Sofija and Vasilev, Saša and Sabljić, Ljiljana and Stojanović, Dušica and Miljković, Đorđe and Dinić, Miroslav and Radojević, Dušan and Jevtić, Bojan and Golić, Nataša and Uskoković, Petar and Sofronić-Milosavljević, Ljiljana and Čolić, Miodrag", year = "2021", abstract = "Trichinella spiralis possess the potential to induce tolerance and restrain excessive immune responses, thus exerting beneficial effect on the outcome of experimental autoimmune encephalomyelitis (EAE), the animal model of human disease multiple sclerosis. Trichinella achieves this by continuous release of its excretoysecretory (ES L1) products in the form of a complex mixture of individual components and extracellular vesicles (TsEVs), preferentially exsosomes. Our previous results suggested that the complex modulation of the host immune response is achieved by ES L1 products or its individula components, which induce the maturation of dendritic cells towards tolerogenic status. A new delivery system, based on nanomaterials as carriers of ES L1, was designed to resemble what happens in nature, ie. spontaneous release of Trichinella products over an extended period of time using a less invasive route of administration than all previously described for the treatment of autoimmune diseases. PLGA biodegradable nanofibers loaded with ES L1 (PLGA-ES L1), were used as subcutaneous implants. The tretment proved to be safe with no side effects, successful in delivering parasite products and mitigating the course of relapsing/remitting EAE in Dark Agouti rats. The mechanisms we examined included events at the systemic level and target tissue, spinal cord, that explain the shift from the pro- to anti-inflammatory responses and success of treatment. PLGA-ES L1 treatment also prevents the EAEinduced disruption of intestinal epithelial barrier. It diminished the inflammation by lowering the expression of TNF-α in gastrointestinal tract (GIT) and succesfully maintain the expression of mRNA for claudin, the most important tight junction protein in GIT. Obseved protective effect was acompained with the re-establishment of gut microbiota diversity that was disturbed in EAE-induced animals.", publisher = "Belgrade : Serbian Society for Parasitology", journal = "Programme & Abstract Book / 13th European Multicolloquium of Parasitology Belgrade, October 12-16", title = "New delivery system for Trichinella spiralis antigens - accelerated approach to autoimmunity treatment", pages = "92", url = "https://hdl.handle.net/21.15107/rcub_technorep_6671" }
Gruden-Movsesijan, A., Tomić, S., Ilić, N., Đokić, J., Glamočlija, S., Vasilev, S., Sabljić, L., Stojanović, D., Miljković, Đ., Dinić, M., Radojević, D., Jevtić, B., Golić, N., Uskoković, P., Sofronić-Milosavljević, L.,& Čolić, M.. (2021). New delivery system for Trichinella spiralis antigens - accelerated approach to autoimmunity treatment. in Programme & Abstract Book / 13th European Multicolloquium of Parasitology Belgrade, October 12-16 Belgrade : Serbian Society for Parasitology., 92. https://hdl.handle.net/21.15107/rcub_technorep_6671
Gruden-Movsesijan A, Tomić S, Ilić N, Đokić J, Glamočlija S, Vasilev S, Sabljić L, Stojanović D, Miljković Đ, Dinić M, Radojević D, Jevtić B, Golić N, Uskoković P, Sofronić-Milosavljević L, Čolić M. New delivery system for Trichinella spiralis antigens - accelerated approach to autoimmunity treatment. in Programme & Abstract Book / 13th European Multicolloquium of Parasitology Belgrade, October 12-16. 2021;:92. https://hdl.handle.net/21.15107/rcub_technorep_6671 .
Gruden-Movsesijan, Alisa, Tomić, Sergej, Ilić, Nataša, Đokić, Jelena, Glamočlija, Sofija, Vasilev, Saša, Sabljić, Ljiljana, Stojanović, Dušica, Miljković, Đorđe, Dinić, Miroslav, Radojević, Dušan, Jevtić, Bojan, Golić, Nataša, Uskoković, Petar, Sofronić-Milosavljević, Ljiljana, Čolić, Miodrag, "New delivery system for Trichinella spiralis antigens - accelerated approach to autoimmunity treatment" in Programme & Abstract Book / 13th European Multicolloquium of Parasitology Belgrade, October 12-16 (2021):92, https://hdl.handle.net/21.15107/rcub_technorep_6671 .