TY  - CONF
AU  - Gruden-Movsesijan, Alisa
AU  - Tomić, Sergej
AU  - Ilić, Nataša
AU  - Đokić, Jelena
AU  - Glamočlija, Sofija
AU  - Vasilev, Saša
AU  - Sabljić, Ljiljana
AU  - Stojanović, Dušica
AU  - Miljković, Đorđe
AU  - Dinić, Miroslav
AU  - Radojević, Dušan
AU  - Jevtić, Bojan
AU  - Golić, Nataša
AU  - Uskoković, Petar
AU  - Sofronić-Milosavljević, Ljiljana
AU  - Čolić, Miodrag
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6671
AB  - Trichinella spiralis possess the potential to induce tolerance and restrain excessive immune responses, thus
exerting beneficial effect on the outcome of experimental autoimmune encephalomyelitis (EAE), the animal
model of human disease multiple sclerosis. Trichinella achieves this by continuous release of its excretoysecretory (ES L1) products in the form of a complex mixture of individual components and extracellular vesicles
(TsEVs), preferentially exsosomes. Our previous results suggested that the complex modulation of the host
immune response is achieved by ES L1 products or its individula components, which induce the maturation
of dendritic cells towards tolerogenic status. A new delivery system, based on nanomaterials as carriers of ES
L1, was designed to resemble what happens in nature, ie. spontaneous release of Trichinella products over
an extended period of time using a less invasive route of administration than all previously described for the
treatment of autoimmune diseases. PLGA biodegradable nanofibers loaded with ES L1 (PLGA-ES L1), were
used as subcutaneous implants. The tretment proved to be safe with no side effects, successful in delivering
parasite products and mitigating the course of relapsing/remitting EAE in Dark Agouti rats. The mechanisms
we examined included events at the systemic level and target tissue, spinal cord, that explain the shift from the
pro- to anti-inflammatory responses and success of treatment. PLGA-ES L1 treatment also prevents the EAEinduced disruption of intestinal epithelial barrier. It diminished the inflammation by lowering the expression
of TNF-α in gastrointestinal tract (GIT) and succesfully maintain the expression of mRNA for claudin, the most
important tight junction protein in GIT. Obseved protective effect was acompained with the re-establishment of gut microbiota diversity that was disturbed in EAE-induced animals.
PB  - Belgrade : Serbian Society for Parasitology
C3  - Programme & Abstract Book / 13th European Multicolloquium of Parasitology Belgrade, October 12-16
T1  - New delivery system for Trichinella spiralis antigens - accelerated approach to autoimmunity treatment
SP  - 92
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6671
ER  - 

@conference{
author = "Gruden-Movsesijan, Alisa and Tomić, Sergej and Ilić, Nataša and Đokić, Jelena and Glamočlija, Sofija and Vasilev, Saša and Sabljić, Ljiljana and Stojanović, Dušica and Miljković, Đorđe and Dinić, Miroslav and Radojević, Dušan and Jevtić, Bojan and Golić, Nataša and Uskoković, Petar and Sofronić-Milosavljević, Ljiljana and Čolić, Miodrag",
year = "2021",
abstract = "Trichinella spiralis possess the potential to induce tolerance and restrain excessive immune responses, thus
exerting beneficial effect on the outcome of experimental autoimmune encephalomyelitis (EAE), the animal
model of human disease multiple sclerosis. Trichinella achieves this by continuous release of its excretoysecretory (ES L1) products in the form of a complex mixture of individual components and extracellular vesicles
(TsEVs), preferentially exsosomes. Our previous results suggested that the complex modulation of the host
immune response is achieved by ES L1 products or its individula components, which induce the maturation
of dendritic cells towards tolerogenic status. A new delivery system, based on nanomaterials as carriers of ES
L1, was designed to resemble what happens in nature, ie. spontaneous release of Trichinella products over
an extended period of time using a less invasive route of administration than all previously described for the
treatment of autoimmune diseases. PLGA biodegradable nanofibers loaded with ES L1 (PLGA-ES L1), were
used as subcutaneous implants. The tretment proved to be safe with no side effects, successful in delivering
parasite products and mitigating the course of relapsing/remitting EAE in Dark Agouti rats. The mechanisms
we examined included events at the systemic level and target tissue, spinal cord, that explain the shift from the
pro- to anti-inflammatory responses and success of treatment. PLGA-ES L1 treatment also prevents the EAEinduced disruption of intestinal epithelial barrier. It diminished the inflammation by lowering the expression
of TNF-α in gastrointestinal tract (GIT) and succesfully maintain the expression of mRNA for claudin, the most
important tight junction protein in GIT. Obseved protective effect was acompained with the re-establishment of gut microbiota diversity that was disturbed in EAE-induced animals.",
publisher = "Belgrade : Serbian Society for Parasitology",
journal = "Programme & Abstract Book / 13th European Multicolloquium of Parasitology Belgrade, October 12-16",
title = "New delivery system for Trichinella spiralis antigens - accelerated approach to autoimmunity treatment",
pages = "92",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6671"
}

Gruden-Movsesijan, A., Tomić, S., Ilić, N., Đokić, J., Glamočlija, S., Vasilev, S., Sabljić, L., Stojanović, D., Miljković, Đ., Dinić, M., Radojević, D., Jevtić, B., Golić, N., Uskoković, P., Sofronić-Milosavljević, L.,& Čolić, M.. (2021). New delivery system for Trichinella spiralis antigens - accelerated approach to autoimmunity treatment. in Programme & Abstract Book / 13th European Multicolloquium of Parasitology Belgrade, October 12-16
Belgrade : Serbian Society for Parasitology., 92.
https://hdl.handle.net/21.15107/rcub_technorep_6671

Gruden-Movsesijan A, Tomić S, Ilić N, Đokić J, Glamočlija S, Vasilev S, Sabljić L, Stojanović D, Miljković Đ, Dinić M, Radojević D, Jevtić B, Golić N, Uskoković P, Sofronić-Milosavljević L, Čolić M. New delivery system for Trichinella spiralis antigens - accelerated approach to autoimmunity treatment. in Programme & Abstract Book / 13th European Multicolloquium of Parasitology Belgrade, October 12-16. 2021;:92.
https://hdl.handle.net/21.15107/rcub_technorep_6671 .

Gruden-Movsesijan, Alisa, Tomić, Sergej, Ilić, Nataša, Đokić, Jelena, Glamočlija, Sofija, Vasilev, Saša, Sabljić, Ljiljana, Stojanović, Dušica, Miljković, Đorđe, Dinić, Miroslav, Radojević, Dušan, Jevtić, Bojan, Golić, Nataša, Uskoković, Petar, Sofronić-Milosavljević, Ljiljana, Čolić, Miodrag, "New delivery system for Trichinella spiralis antigens - accelerated approach to autoimmunity treatment" in Programme & Abstract Book / 13th European Multicolloquium of Parasitology Belgrade, October 12-16 (2021):92,
https://hdl.handle.net/21.15107/rcub_technorep_6671 .

TY  - JOUR
AU  - Čolić, Miodrag
AU  - Dzopalić, Tanja
AU  - Tomić, Sergej
AU  - Rajković, Jelena
AU  - Rudolf, Rebeka
AU  - Vuković, Goran D.
AU  - Marinković, Aleksandar
AU  - Uskoković, Petar
PY  - 2014
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2782
AB  - The protocols for the preparation of dendritic cells (DCs) as cancer vaccines based on stimulation of Toll-like receptors (TLRs) are very promising. In this study we covalently attached 7-thia-8-oxoguanosine (7-TOG), a selective endosomal TLR7, to previously oxidized multi-walled carbon nanotubes (o-MWCNTs) (mass ratio 1:5.6, respectively), and tested their ability to activate human monocyte-derived (Mo)DCs. Light, confocal and transmission electron microscopy confirmed efficient phagocytosis of 7-TOG-MWCNTs by MoDCs and their efficient delivery to TLR7(+) endosomes. The biocompatibility studies showed that 7-TOG-MWCNTs, at concentrations lower than 100 mu g/ml, were not cytotoxic for MoDCs. 7-TOG-MV/CNTs (50 mu g/ml) up-regulated CD86 expression, allostimulatory activity, T helper (Th)1- and Th17-polarizing capability of MoDCs. The same concentration of soluble 7-TOG, alone or in combination with control o-MWCNTs, did not have such effects. It can be hypothesized that the efficacy of 7-TOG-MWCNTs in stimulating MoDCs was a consequence of increased intracellular concentration of 7-TOG after internalization of the nano-complexes, because similar Th-polarizing capability could be induced with 10-times higher concentrations of soluble 7-TOG. In conclusion, our results suggest that functionalized MWCNTs may be a promising system for the delivery of drugs to intracellular targets, in order to improve the immunogenic potential of DCs for therapeutic purposes.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Carbon
T1  - Immunomodulatory effects of carbon nanotubes functionalized with a Toll-like receptor 7 agonist on human dendritic cells
EP  - 287
SP  - 273
VL  - 67
DO  - 10.1016/j.carbon.2013.09.090
ER  - 

@article{
author = "Čolić, Miodrag and Dzopalić, Tanja and Tomić, Sergej and Rajković, Jelena and Rudolf, Rebeka and Vuković, Goran D. and Marinković, Aleksandar and Uskoković, Petar",
year = "2014",
abstract = "The protocols for the preparation of dendritic cells (DCs) as cancer vaccines based on stimulation of Toll-like receptors (TLRs) are very promising. In this study we covalently attached 7-thia-8-oxoguanosine (7-TOG), a selective endosomal TLR7, to previously oxidized multi-walled carbon nanotubes (o-MWCNTs) (mass ratio 1:5.6, respectively), and tested their ability to activate human monocyte-derived (Mo)DCs. Light, confocal and transmission electron microscopy confirmed efficient phagocytosis of 7-TOG-MWCNTs by MoDCs and their efficient delivery to TLR7(+) endosomes. The biocompatibility studies showed that 7-TOG-MWCNTs, at concentrations lower than 100 mu g/ml, were not cytotoxic for MoDCs. 7-TOG-MV/CNTs (50 mu g/ml) up-regulated CD86 expression, allostimulatory activity, T helper (Th)1- and Th17-polarizing capability of MoDCs. The same concentration of soluble 7-TOG, alone or in combination with control o-MWCNTs, did not have such effects. It can be hypothesized that the efficacy of 7-TOG-MWCNTs in stimulating MoDCs was a consequence of increased intracellular concentration of 7-TOG after internalization of the nano-complexes, because similar Th-polarizing capability could be induced with 10-times higher concentrations of soluble 7-TOG. In conclusion, our results suggest that functionalized MWCNTs may be a promising system for the delivery of drugs to intracellular targets, in order to improve the immunogenic potential of DCs for therapeutic purposes.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Carbon",
title = "Immunomodulatory effects of carbon nanotubes functionalized with a Toll-like receptor 7 agonist on human dendritic cells",
pages = "287-273",
volume = "67",
doi = "10.1016/j.carbon.2013.09.090"
}

Čolić, M., Dzopalić, T., Tomić, S., Rajković, J., Rudolf, R., Vuković, G. D., Marinković, A.,& Uskoković, P.. (2014). Immunomodulatory effects of carbon nanotubes functionalized with a Toll-like receptor 7 agonist on human dendritic cells. in Carbon
Pergamon-Elsevier Science Ltd, Oxford., 67, 273-287.
https://doi.org/10.1016/j.carbon.2013.09.090

Čolić M, Dzopalić T, Tomić S, Rajković J, Rudolf R, Vuković GD, Marinković A, Uskoković P. Immunomodulatory effects of carbon nanotubes functionalized with a Toll-like receptor 7 agonist on human dendritic cells. in Carbon. 2014;67:273-287.
doi:10.1016/j.carbon.2013.09.090 .

Čolić, Miodrag, Dzopalić, Tanja, Tomić, Sergej, Rajković, Jelena, Rudolf, Rebeka, Vuković, Goran D., Marinković, Aleksandar, Uskoković, Petar, "Immunomodulatory effects of carbon nanotubes functionalized with a Toll-like receptor 7 agonist on human dendritic cells" in Carbon, 67 (2014):273-287,
https://doi.org/10.1016/j.carbon.2013.09.090 . .

TY  - JOUR
AU  - Vuković, Goran D.
AU  - Tomić, Sergej
AU  - Marinković, Aleksandar
AU  - Radmilović, Velimir R.
AU  - Uskoković, Petar
AU  - Čolić, Miodrag
PY  - 2010
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1648
AB  - Dapsone is an anti-microbial and anti-inflammatory drug. Water-dispersible dapsone-modified multi-wall carbon nanotubes (dap-MWCNTs) were prepared by chemical modification of the carboxyl groups introduced on the surface of the nanotubes using O-(7-aza-benzotriazol-1-yl)-N,N,N',N'-tetramethyluronium hexafluorophosphate (N-HATU) and N,N-diisopropylethylamine (DIEA). The modification was confirmed by Fourier-transform infrared spectroscopy, transmission election microscopy and thermogravimetric analysis. The biological effect of dap-MWCNTs was tested using rat peritoneal macrophages (PMempty set). By confocal laser microscopy and flow cytometry, it was shown that the dap-MWCNTs were rapidly ingested by PMempty set as were the control, oxidized o-MWCNTs. Neither dap-MWCNTs at lower concentrations (up to 50 mu g/ml), nor o-MWCNTs, at equivalent concentrations, respectively affected the viability of PMempty set, while higher concentrations triggered apoptosis. Apoptosis of PMempty set induced by the control, o-MWCNTs, was higher than that induced by dap-MWCNTs and it correlated with the induction of oxidative stress. In contrast, dap-MWCNTs did not trigger oxidative stress but caused apoptosis of PMempty set predominantly after prolonged cultivation (3 days). Although equivalent concentrations of soluble dapsone induced oxidative stress, they were anti-apoptotic. Cumulatively, the obtained results show the complexity of dap-MWCNT/PMempty set interactions and suggest that this complex could be investigated for the treatment of dapsone-sensitive intracellular microorganisms or inflammatory diseases responding to dapsone therapy.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Carbon
T1  - The response of peritoneal macrophages to dapsone covalently attached on the surface of carbon nanotubes
EP  - 3078
IS  - 11
SP  - 3066
VL  - 48
DO  - 10.1016/j.carbon.2010.04.043
ER  - 

@article{
author = "Vuković, Goran D. and Tomić, Sergej and Marinković, Aleksandar and Radmilović, Velimir R. and Uskoković, Petar and Čolić, Miodrag",
year = "2010",
abstract = "Dapsone is an anti-microbial and anti-inflammatory drug. Water-dispersible dapsone-modified multi-wall carbon nanotubes (dap-MWCNTs) were prepared by chemical modification of the carboxyl groups introduced on the surface of the nanotubes using O-(7-aza-benzotriazol-1-yl)-N,N,N',N'-tetramethyluronium hexafluorophosphate (N-HATU) and N,N-diisopropylethylamine (DIEA). The modification was confirmed by Fourier-transform infrared spectroscopy, transmission election microscopy and thermogravimetric analysis. The biological effect of dap-MWCNTs was tested using rat peritoneal macrophages (PMempty set). By confocal laser microscopy and flow cytometry, it was shown that the dap-MWCNTs were rapidly ingested by PMempty set as were the control, oxidized o-MWCNTs. Neither dap-MWCNTs at lower concentrations (up to 50 mu g/ml), nor o-MWCNTs, at equivalent concentrations, respectively affected the viability of PMempty set, while higher concentrations triggered apoptosis. Apoptosis of PMempty set induced by the control, o-MWCNTs, was higher than that induced by dap-MWCNTs and it correlated with the induction of oxidative stress. In contrast, dap-MWCNTs did not trigger oxidative stress but caused apoptosis of PMempty set predominantly after prolonged cultivation (3 days). Although equivalent concentrations of soluble dapsone induced oxidative stress, they were anti-apoptotic. Cumulatively, the obtained results show the complexity of dap-MWCNT/PMempty set interactions and suggest that this complex could be investigated for the treatment of dapsone-sensitive intracellular microorganisms or inflammatory diseases responding to dapsone therapy.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Carbon",
title = "The response of peritoneal macrophages to dapsone covalently attached on the surface of carbon nanotubes",
pages = "3078-3066",
number = "11",
volume = "48",
doi = "10.1016/j.carbon.2010.04.043"
}

Vuković, G. D., Tomić, S., Marinković, A., Radmilović, V. R., Uskoković, P.,& Čolić, M.. (2010). The response of peritoneal macrophages to dapsone covalently attached on the surface of carbon nanotubes. in Carbon
Pergamon-Elsevier Science Ltd, Oxford., 48(11), 3066-3078.
https://doi.org/10.1016/j.carbon.2010.04.043

Vuković GD, Tomić S, Marinković A, Radmilović VR, Uskoković P, Čolić M. The response of peritoneal macrophages to dapsone covalently attached on the surface of carbon nanotubes. in Carbon. 2010;48(11):3066-3078.
doi:10.1016/j.carbon.2010.04.043 .

Vuković, Goran D., Tomić, Sergej, Marinković, Aleksandar, Radmilović, Velimir R., Uskoković, Petar, Čolić, Miodrag, "The response of peritoneal macrophages to dapsone covalently attached on the surface of carbon nanotubes" in Carbon, 48, no. 11 (2010):3066-3078,
https://doi.org/10.1016/j.carbon.2010.04.043 . .