TY  - JOUR
AU  - Batinić, Petar M.
AU  - Đorđević, Verica B.
AU  - Obradović, Nataša S.
AU  - Krstić, Aleksandar D.
AU  - Stevanović, Sanja I.
AU  - Balanč, Bojana D.
AU  - Marković, Smilja B.
AU  - Pjanović, Rada V.
AU  - Mijin, Dušan Ž.
AU  - Bugarski, Branko M.
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6358
AB  - Liposome-encapsulated folic acid is incorporated into the films made from sodium carboxymethyl cellulose (CMC) (2 mas%) and a mixture of CMC and solagum (9:1 w/w) using the film-forming cast solution method. Histidine is used to increase solubility for folic acid in liposomes (1–5 mg mL−1), and propylene glycol is used as a film plasticizer (2.6 mas%). The obtained films (50–60 µm tick) containing 3.12–20.19 mg of folic acid per gram of film are envisaged to be used as patches for transdermal delivery of folic acid. Therefore, some physical, mechanical, release and structural attributes of the films are scrutinized. Folic acid gives yellow color to the films and contributes to stronger chemical bonds which result in improved strength of the film. Liposomes prolong the release of folic acid from films to 24 h without adverse effects on mechanical properties of the films, but degrade homogeneity of the films, which can be ascribed to its agglomeration within the film matrix as revealed by atomic force microscopy. According to the release at pH 5.5, the film formulation based on a blend of CMC and solagum containing 3 mg mL−1 liposome-encapsulated folic acid is recommended. Practical Application: Folic acid is effective in reducing oxidative stress levels in the skin and neutralizing the harmful free radicals and is also essential for various metabolic reactions in the body. However, the limited solubility of folic acid linked with its poor absorption in an organism, low storage stability, short half-life upon oral consumption, specific food preferences of some people, extensive liver metabolism, and pregnancy-induced vomiting point to a large potential in transdermal usage of folic acid. This has motivated us to design new multicomponent polymer-lipid systems as an alternative solution to overcome some of these drawbacks. The results obtained for these multicomponent films pointed to their potential for prolonged release of folic acid to 24 h, which can also be useful for scientists interested in encapsulating similar poorly soluble compounds in CMC patches. The finding can be also valuable information for pharmaceutical manufacturers and scientists worldwide.
PB  - John Wiley and Sons Inc.
T2  - European Journal of Lipid Science and Technology
T1  - Polymer-Lipid Matrices based on Carboxymethyl Cellulose/Solagum and Liposomes for Controlled Release of Folic Acid
SP  - 2200169
DO  - 10.1002/ejlt.202200169
ER  - 

@article{
author = "Batinić, Petar M. and Đorđević, Verica B. and Obradović, Nataša S. and Krstić, Aleksandar D. and Stevanović, Sanja I. and Balanč, Bojana D. and Marković, Smilja B. and Pjanović, Rada V. and Mijin, Dušan Ž. and Bugarski, Branko M.",
year = "2023",
abstract = "Liposome-encapsulated folic acid is incorporated into the films made from sodium carboxymethyl cellulose (CMC) (2 mas%) and a mixture of CMC and solagum (9:1 w/w) using the film-forming cast solution method. Histidine is used to increase solubility for folic acid in liposomes (1–5 mg mL−1), and propylene glycol is used as a film plasticizer (2.6 mas%). The obtained films (50–60 µm tick) containing 3.12–20.19 mg of folic acid per gram of film are envisaged to be used as patches for transdermal delivery of folic acid. Therefore, some physical, mechanical, release and structural attributes of the films are scrutinized. Folic acid gives yellow color to the films and contributes to stronger chemical bonds which result in improved strength of the film. Liposomes prolong the release of folic acid from films to 24 h without adverse effects on mechanical properties of the films, but degrade homogeneity of the films, which can be ascribed to its agglomeration within the film matrix as revealed by atomic force microscopy. According to the release at pH 5.5, the film formulation based on a blend of CMC and solagum containing 3 mg mL−1 liposome-encapsulated folic acid is recommended. Practical Application: Folic acid is effective in reducing oxidative stress levels in the skin and neutralizing the harmful free radicals and is also essential for various metabolic reactions in the body. However, the limited solubility of folic acid linked with its poor absorption in an organism, low storage stability, short half-life upon oral consumption, specific food preferences of some people, extensive liver metabolism, and pregnancy-induced vomiting point to a large potential in transdermal usage of folic acid. This has motivated us to design new multicomponent polymer-lipid systems as an alternative solution to overcome some of these drawbacks. The results obtained for these multicomponent films pointed to their potential for prolonged release of folic acid to 24 h, which can also be useful for scientists interested in encapsulating similar poorly soluble compounds in CMC patches. The finding can be also valuable information for pharmaceutical manufacturers and scientists worldwide.",
publisher = "John Wiley and Sons Inc.",
journal = "European Journal of Lipid Science and Technology",
title = "Polymer-Lipid Matrices based on Carboxymethyl Cellulose/Solagum and Liposomes for Controlled Release of Folic Acid",
pages = "2200169",
doi = "10.1002/ejlt.202200169"
}

Batinić, P. M., Đorđević, V. B., Obradović, N. S., Krstić, A. D., Stevanović, S. I., Balanč, B. D., Marković, S. B., Pjanović, R. V., Mijin, D. Ž.,& Bugarski, B. M.. (2023). Polymer-Lipid Matrices based on Carboxymethyl Cellulose/Solagum and Liposomes for Controlled Release of Folic Acid. in European Journal of Lipid Science and Technology
John Wiley and Sons Inc.., 2200169.
https://doi.org/10.1002/ejlt.202200169

Batinić PM, Đorđević VB, Obradović NS, Krstić AD, Stevanović SI, Balanč BD, Marković SB, Pjanović RV, Mijin DŽ, Bugarski BM. Polymer-Lipid Matrices based on Carboxymethyl Cellulose/Solagum and Liposomes for Controlled Release of Folic Acid. in European Journal of Lipid Science and Technology. 2023;:2200169.
doi:10.1002/ejlt.202200169 .

Batinić, Petar M., Đorđević, Verica B., Obradović, Nataša S., Krstić, Aleksandar D., Stevanović, Sanja I., Balanč, Bojana D., Marković, Smilja B., Pjanović, Rada V., Mijin, Dušan Ž., Bugarski, Branko M., "Polymer-Lipid Matrices based on Carboxymethyl Cellulose/Solagum and Liposomes for Controlled Release of Folic Acid" in European Journal of Lipid Science and Technology (2023):2200169,
https://doi.org/10.1002/ejlt.202200169 . .

TY  - CONF
AU  - Jovanović, Aleksandra
AU  - Volić, Mina
AU  - Obradović, Nataša
AU  - Bugarski, Branko
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6452
AB  - Polyphenol recovery and physico-chemical properties of the extracts depend on the type of
solvents and their pH values. Additionally, due to the presence of different biologically active
compounds in plant waste, plant extracts obtained from industrial by-processing can find
potential applications in various products. In the present study, Serpylli herba waste extracts
were prepared using maceration (60 min), a solid-to-solvent ratio of 1:30, the particle size of
plant waste 0.3 mm, and two types of the extraction medium: 50% ethanol (pH 6) and 50%
ethanol with glacial acetic acid (pH 2.5). The lyophilization process was chosen as the next
step (-75°C, 0.011 mbar, for 24 h). Comparison of liquid and lyophilized extracts prepared at
different pH values was done via analyzing total polyphenol content (TPC, Folin-Ciocalteu
method), total flavonoid content (TFC, colorimetric assay), antioxidant capacity (ABTS and
DPPH assays), zeta potential, and conductivity (photon correlation spectroscopy). TPC of
liquid extracts prepared at pH 2.5 and pH 6 amounted to 1.38 and 1.23 mg GAE/mL,
respectively, while lyophilized parallels had 271.7 and 188.8 mg GAE/g, respectively. The
same trend is noticed in the case of TFC: 0.368 and 0.334 mg CE/mL for liquid extracts
obtained at pH 2.5 and pH 6, respectively, and 102.5 and 99.7 mg CE/mL for lyophilized
parallels. ABTS radical scavenging activity of the liquid extracts at pH 2.5 and pH 6 was
0.767 and 0.750 mmol TE/L, respectively and for the lyophilized parallels was 0.136 and
0.111 mmol TE/g. IC50 (concentration for neutralization of 50% of DPPH free radicals) was
1.12 and 1.75 mg/mL for the liquid extracts prepared at pH 2.5 and pH 6, respectively and
0.331 and 0.391 mg/mL for the lyophilized parallels. The zeta potential of the liquid extracts
at pH 2.5 and pH 6 was 1.74 and -2.56 mV, respectively, whereas the zeta potential of
lyophilized parallels was -3.55 and -18.7 mV. Conductivity of the liquid extracts was 0.864(pH 2.5) and 0.423 mS/cm (pH 6), whereas for the lyophilized extracts it was 0.199 (pH 2.5)
and 0.452 mS/cm (pH 6). The presented results provide the information on physico-chemical
properties of Serpylli herba waste liquid and lyophilized extracts that can add value and
improve the quality of the existing food, functional food, pharmaceutical and cosmetic
products, as well as for drinking water and wastewater treatment.
PB  - Novi Sad : Faculty of Technology
C3  - Proceedings of the 2nd International Conference on Advanced Production and Processing ICAPP 2022, Novi Sad
T1  - COMPARISON OF LIQUID AND LYOPHILIZED SERPYLLI HERBA WASTE EXTRACTS PREPARED AT DIFFERENT pH VALUES
EP  - 175
SP  - 166
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6452
ER  - 

@conference{
author = "Jovanović, Aleksandra and Volić, Mina and Obradović, Nataša and Bugarski, Branko",
year = "2023",
abstract = "Polyphenol recovery and physico-chemical properties of the extracts depend on the type of
solvents and their pH values. Additionally, due to the presence of different biologically active
compounds in plant waste, plant extracts obtained from industrial by-processing can find
potential applications in various products. In the present study, Serpylli herba waste extracts
were prepared using maceration (60 min), a solid-to-solvent ratio of 1:30, the particle size of
plant waste 0.3 mm, and two types of the extraction medium: 50% ethanol (pH 6) and 50%
ethanol with glacial acetic acid (pH 2.5). The lyophilization process was chosen as the next
step (-75°C, 0.011 mbar, for 24 h). Comparison of liquid and lyophilized extracts prepared at
different pH values was done via analyzing total polyphenol content (TPC, Folin-Ciocalteu
method), total flavonoid content (TFC, colorimetric assay), antioxidant capacity (ABTS and
DPPH assays), zeta potential, and conductivity (photon correlation spectroscopy). TPC of
liquid extracts prepared at pH 2.5 and pH 6 amounted to 1.38 and 1.23 mg GAE/mL,
respectively, while lyophilized parallels had 271.7 and 188.8 mg GAE/g, respectively. The
same trend is noticed in the case of TFC: 0.368 and 0.334 mg CE/mL for liquid extracts
obtained at pH 2.5 and pH 6, respectively, and 102.5 and 99.7 mg CE/mL for lyophilized
parallels. ABTS radical scavenging activity of the liquid extracts at pH 2.5 and pH 6 was
0.767 and 0.750 mmol TE/L, respectively and for the lyophilized parallels was 0.136 and
0.111 mmol TE/g. IC50 (concentration for neutralization of 50% of DPPH free radicals) was
1.12 and 1.75 mg/mL for the liquid extracts prepared at pH 2.5 and pH 6, respectively and
0.331 and 0.391 mg/mL for the lyophilized parallels. The zeta potential of the liquid extracts
at pH 2.5 and pH 6 was 1.74 and -2.56 mV, respectively, whereas the zeta potential of
lyophilized parallels was -3.55 and -18.7 mV. Conductivity of the liquid extracts was 0.864(pH 2.5) and 0.423 mS/cm (pH 6), whereas for the lyophilized extracts it was 0.199 (pH 2.5)
and 0.452 mS/cm (pH 6). The presented results provide the information on physico-chemical
properties of Serpylli herba waste liquid and lyophilized extracts that can add value and
improve the quality of the existing food, functional food, pharmaceutical and cosmetic
products, as well as for drinking water and wastewater treatment.",
publisher = "Novi Sad : Faculty of Technology",
journal = "Proceedings of the 2nd International Conference on Advanced Production and Processing ICAPP 2022, Novi Sad",
title = "COMPARISON OF LIQUID AND LYOPHILIZED SERPYLLI HERBA WASTE EXTRACTS PREPARED AT DIFFERENT pH VALUES",
pages = "175-166",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6452"
}

Jovanović, A., Volić, M., Obradović, N.,& Bugarski, B.. (2023). COMPARISON OF LIQUID AND LYOPHILIZED SERPYLLI HERBA WASTE EXTRACTS PREPARED AT DIFFERENT pH VALUES. in Proceedings of the 2nd International Conference on Advanced Production and Processing ICAPP 2022, Novi Sad
Novi Sad : Faculty of Technology., 166-175.
https://hdl.handle.net/21.15107/rcub_technorep_6452

Jovanović A, Volić M, Obradović N, Bugarski B. COMPARISON OF LIQUID AND LYOPHILIZED SERPYLLI HERBA WASTE EXTRACTS PREPARED AT DIFFERENT pH VALUES. in Proceedings of the 2nd International Conference on Advanced Production and Processing ICAPP 2022, Novi Sad. 2023;:166-175.
https://hdl.handle.net/21.15107/rcub_technorep_6452 .

Jovanović, Aleksandra, Volić, Mina, Obradović, Nataša, Bugarski, Branko, "COMPARISON OF LIQUID AND LYOPHILIZED SERPYLLI HERBA WASTE EXTRACTS PREPARED AT DIFFERENT pH VALUES" in Proceedings of the 2nd International Conference on Advanced Production and Processing ICAPP 2022, Novi Sad (2023):166-175,
https://hdl.handle.net/21.15107/rcub_technorep_6452 .

TY  - CONF
AU  - Jovanović, Aleksandra A.
AU  - Petrović, Predrag
AU  - Volić, Mina
AU  - Obradović, Nataša
AU  - Balanč, Bojana
AU  - Pravilović, Radoslava
AU  - Bugarski, Branko
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6076
AB  - In the present study, Serpylli herba's waste extracts were prepared using 50% ethanol (pH 6) and 50%
ethanol with hydrochloric acid (pH 1.2), using maceration (25 °C) and heat-assisted extraction (HAE,
80 °C). The best extraction conditions were determined via analyzing total polyphenol (TPC) and
flavonoid contents (TFC), antioxidant capacity, extraction yield, zeta potential, density, and surface
tension. In maceration and HAE, TPC were 1.18±0.06 and 1.43±0.05 mg GAE/mL (pH 6), and
0.69±0.05 and 0.86±0.02 mg GAE/mL (pH 1.2). The same trend is noticed in the case of TFC:
0.33±0.01 and 0.42±0.02 mg CE/mL (pH 6) and 0.24±0.01 and 0.27±0.01 mg CE/mL (pH 1.2). ABTS
radical scavenging activity was 775.0±3.5 and 623.8±5.1 mmol Trolox/mL (pH 6), and 269.2±3.1
and 327.9±7.7 mmol Trolox/mL (pH 1.2). In DPPH assay, IC50 was 1.64±0.04 and 1.48±0.03 mg/mL
(pH 6), and 7.59±0.07 and 5.11±0.09 mg/mL (pH 1.2). Extraction yield was 0.541±0.049 and
0.583±0.019% (pH 6), and 0.938±0.057 and 0.889±0.060% (pH 1.2). Zeta potential was -2.58±0.02
and -2.08±0.10 mV (pH 6), and 1.49±0.37 and 1.08±0.09 mV (pH 1.2). Density was 0.945±0.001
and 0.949±0.001 g/mL (pH 6), and 1.008±0.001 and 1.010±0.001 g/mL (pH 1.2). Surface tension
was 29.2±0.3 and 30.5±1.0 mN/m (pH 6), and 32.6±2.3 and 36.5±2.3 mN/m (pH 1.2). The best
properties were determined in the extract obtained in HAE at pH 6. The aim of this study is to provide
evidence of the physico-chemical properties of Serpylli herba's waste extracts that can add value and
improve the quality of existing food, pharmaceutical, and cosmetic products.
PB  - University in Banjaluka, Faculty of Technology
C3  - XIV CONFERENCE OF CHEMISTS, TECHNOLOGISTS AND
ENVIRONMENTALISTS OF REPUBLIC OF SRPSKA
BOOK OF PROCEEDINGS, OCTOBER 21-22, 2022, BANJA LUKA
T1  - The impact of pH value and temperature on polyphenol recovery, antioxidant capacity and physical properties of Serpylli herba's waste extracts
EP  - 104
SP  - 96
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6076
ER  - 

@conference{
author = "Jovanović, Aleksandra A. and Petrović, Predrag and Volić, Mina and Obradović, Nataša and Balanč, Bojana and Pravilović, Radoslava and Bugarski, Branko",
year = "2023",
abstract = "In the present study, Serpylli herba's waste extracts were prepared using 50% ethanol (pH 6) and 50%
ethanol with hydrochloric acid (pH 1.2), using maceration (25 °C) and heat-assisted extraction (HAE,
80 °C). The best extraction conditions were determined via analyzing total polyphenol (TPC) and
flavonoid contents (TFC), antioxidant capacity, extraction yield, zeta potential, density, and surface
tension. In maceration and HAE, TPC were 1.18±0.06 and 1.43±0.05 mg GAE/mL (pH 6), and
0.69±0.05 and 0.86±0.02 mg GAE/mL (pH 1.2). The same trend is noticed in the case of TFC:
0.33±0.01 and 0.42±0.02 mg CE/mL (pH 6) and 0.24±0.01 and 0.27±0.01 mg CE/mL (pH 1.2). ABTS
radical scavenging activity was 775.0±3.5 and 623.8±5.1 mmol Trolox/mL (pH 6), and 269.2±3.1
and 327.9±7.7 mmol Trolox/mL (pH 1.2). In DPPH assay, IC50 was 1.64±0.04 and 1.48±0.03 mg/mL
(pH 6), and 7.59±0.07 and 5.11±0.09 mg/mL (pH 1.2). Extraction yield was 0.541±0.049 and
0.583±0.019% (pH 6), and 0.938±0.057 and 0.889±0.060% (pH 1.2). Zeta potential was -2.58±0.02
and -2.08±0.10 mV (pH 6), and 1.49±0.37 and 1.08±0.09 mV (pH 1.2). Density was 0.945±0.001
and 0.949±0.001 g/mL (pH 6), and 1.008±0.001 and 1.010±0.001 g/mL (pH 1.2). Surface tension
was 29.2±0.3 and 30.5±1.0 mN/m (pH 6), and 32.6±2.3 and 36.5±2.3 mN/m (pH 1.2). The best
properties were determined in the extract obtained in HAE at pH 6. The aim of this study is to provide
evidence of the physico-chemical properties of Serpylli herba's waste extracts that can add value and
improve the quality of existing food, pharmaceutical, and cosmetic products.",
publisher = "University in Banjaluka, Faculty of Technology",
journal = "XIV CONFERENCE OF CHEMISTS, TECHNOLOGISTS AND
ENVIRONMENTALISTS OF REPUBLIC OF SRPSKA
BOOK OF PROCEEDINGS, OCTOBER 21-22, 2022, BANJA LUKA",
title = "The impact of pH value and temperature on polyphenol recovery, antioxidant capacity and physical properties of Serpylli herba's waste extracts",
pages = "104-96",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6076"
}

Jovanović, A. A., Petrović, P., Volić, M., Obradović, N., Balanč, B., Pravilović, R.,& Bugarski, B.. (2023). The impact of pH value and temperature on polyphenol recovery, antioxidant capacity and physical properties of Serpylli herba's waste extracts. in XIV CONFERENCE OF CHEMISTS, TECHNOLOGISTS AND
ENVIRONMENTALISTS OF REPUBLIC OF SRPSKA
BOOK OF PROCEEDINGS, OCTOBER 21-22, 2022, BANJA LUKA
University in Banjaluka, Faculty of Technology., 96-104.
https://hdl.handle.net/21.15107/rcub_technorep_6076

Jovanović AA, Petrović P, Volić M, Obradović N, Balanč B, Pravilović R, Bugarski B. The impact of pH value and temperature on polyphenol recovery, antioxidant capacity and physical properties of Serpylli herba's waste extracts. in XIV CONFERENCE OF CHEMISTS, TECHNOLOGISTS AND
ENVIRONMENTALISTS OF REPUBLIC OF SRPSKA
BOOK OF PROCEEDINGS, OCTOBER 21-22, 2022, BANJA LUKA. 2023;:96-104.
https://hdl.handle.net/21.15107/rcub_technorep_6076 .

Jovanović, Aleksandra A., Petrović, Predrag, Volić, Mina, Obradović, Nataša, Balanč, Bojana, Pravilović, Radoslava, Bugarski, Branko, "The impact of pH value and temperature on polyphenol recovery, antioxidant capacity and physical properties of Serpylli herba's waste extracts" in XIV CONFERENCE OF CHEMISTS, TECHNOLOGISTS AND
ENVIRONMENTALISTS OF REPUBLIC OF SRPSKA
BOOK OF PROCEEDINGS, OCTOBER 21-22, 2022, BANJA LUKA (2023):96-104,
https://hdl.handle.net/21.15107/rcub_technorep_6076 .

TY  - JOUR
AU  - Galić, Aleksa
AU  - Matić, Tamara
AU  - Obradović, Nataša
AU  - Baščarević, Zvezdana
AU  - Veljović, Đorđe
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7282
AB  - Limited bone bank capacity and risk of infection are some of the main drawbacks of autologous and allogenic grafts, giving rise to synthetic materials for bone tissue implants. The aim of this study was to process and evaluate the mechanical properties and bioactivity of magnesium and strontium doped hydroxyapatite (HAp) scaffolds and investigate the effect of adding zirconium oxide and gelatine coating the scaffolds. Doped nanosized hydroxyapatite powder was synthesized by the hydrothermal method and the scaffolds were made by the foam replica technique and sintered at different temperatures. Yttria-stabilized zirconium oxide (YSZ), synthesized by plasma technology, was used as reinforcement of calcium phosphate scaffolds. Element analysis, phase composition, morphology of the powders and microstructure of the scaffolds were investigated, as well as the compressive strength of the coated and uncoated scaffolds and bioactivity in simulated body fluid (SBF). A microporous structure was achieved with interconnected pores and bioactivity in SBF was confirmed in all cases. The best mechanical properties were given by the coated composite HAp/YSZ scaffolds, withstanding average stresses of over 1019 kPa. These results encourage the idea of use of these scaffolds in bone regenerative therapy and bone tissue engineering.
PB  - International Institute for the Science of Sintering
T2  - Science of Sintering
T1  - Processing of gelatine coated composite scaffolds based on magnesium and strontium doped hydroxyapatite and yttria-stabilized zirconium oxide
EP  - 479
IS  - 4
SP  - 469
VL  - 55
DO  - 10.2298/SOS220723019G
ER  - 

@article{
author = "Galić, Aleksa and Matić, Tamara and Obradović, Nataša and Baščarević, Zvezdana and Veljović, Đorđe",
year = "2023",
abstract = "Limited bone bank capacity and risk of infection are some of the main drawbacks of autologous and allogenic grafts, giving rise to synthetic materials for bone tissue implants. The aim of this study was to process and evaluate the mechanical properties and bioactivity of magnesium and strontium doped hydroxyapatite (HAp) scaffolds and investigate the effect of adding zirconium oxide and gelatine coating the scaffolds. Doped nanosized hydroxyapatite powder was synthesized by the hydrothermal method and the scaffolds were made by the foam replica technique and sintered at different temperatures. Yttria-stabilized zirconium oxide (YSZ), synthesized by plasma technology, was used as reinforcement of calcium phosphate scaffolds. Element analysis, phase composition, morphology of the powders and microstructure of the scaffolds were investigated, as well as the compressive strength of the coated and uncoated scaffolds and bioactivity in simulated body fluid (SBF). A microporous structure was achieved with interconnected pores and bioactivity in SBF was confirmed in all cases. The best mechanical properties were given by the coated composite HAp/YSZ scaffolds, withstanding average stresses of over 1019 kPa. These results encourage the idea of use of these scaffolds in bone regenerative therapy and bone tissue engineering.",
publisher = "International Institute for the Science of Sintering",
journal = "Science of Sintering",
title = "Processing of gelatine coated composite scaffolds based on magnesium and strontium doped hydroxyapatite and yttria-stabilized zirconium oxide",
pages = "479-469",
number = "4",
volume = "55",
doi = "10.2298/SOS220723019G"
}

Galić, A., Matić, T., Obradović, N., Baščarević, Z.,& Veljović, Đ.. (2023). Processing of gelatine coated composite scaffolds based on magnesium and strontium doped hydroxyapatite and yttria-stabilized zirconium oxide. in Science of Sintering
International Institute for the Science of Sintering., 55(4), 469-479.
https://doi.org/10.2298/SOS220723019G

Galić A, Matić T, Obradović N, Baščarević Z, Veljović Đ. Processing of gelatine coated composite scaffolds based on magnesium and strontium doped hydroxyapatite and yttria-stabilized zirconium oxide. in Science of Sintering. 2023;55(4):469-479.
doi:10.2298/SOS220723019G .

Galić, Aleksa, Matić, Tamara, Obradović, Nataša, Baščarević, Zvezdana, Veljović, Đorđe, "Processing of gelatine coated composite scaffolds based on magnesium and strontium doped hydroxyapatite and yttria-stabilized zirconium oxide" in Science of Sintering, 55, no. 4 (2023):469-479,
https://doi.org/10.2298/SOS220723019G . .

TY  - CHAP
AU  - Đorđević, Verica
AU  - Balanč, Bojana
AU  - Kalušević, Ana
AU  - Volić, Mina
AU  - Obradović, Nataša
AU  - Petrović, Predrag
AU  - Bugarski, Branko
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6128
AB  - The controlled active molecule-delivery technology has progressed over the last decades, with the development of sustained release systems for application in food sector. It is useful to control the rate and extent of food digestion in different regions of the gastrointestinal tract (GIT) and release of an active compound with the ultimate goal of controlling bioavailability and satiety of blood levels. The nature, molecular weight and concentration of a carrier material, as well as the lipophilic/hydrophilic character of the loaded active component, its concentration, interfacial properties and the particle size distribution are some of the key factors that determine the delivery performance of these systems. pH variation, temperature change, variation of the redox potential and enzyme activity are stimuli which most often induce the release of an active molecule in a medium. In order to develop food additives with controlled release, their processing in the gastrointestinal tract has to be understood; therefore, a general description of the digestive system is given and associated to the currently used in vitro experimental models.

This chapter aims to give a useful overview on the gastrointestinal fate of the most frequently used carrier materials and structures of food-grade delivery systems. At the end, mathematical models most useful in fitting to the experimental data of release profiles and resolving the mechanisms implied in controlled release are summarized.
PB  - CRC Press
T2  - Encapsulation in Food Processing and Fermentation
T1  - Controlled Release Systems for Food Application
EP  - 171
SP  - 142
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6128
ER  - 

@inbook{
author = "Đorđević, Verica and Balanč, Bojana and Kalušević, Ana and Volić, Mina and Obradović, Nataša and Petrović, Predrag and Bugarski, Branko",
year = "2022",
abstract = "The controlled active molecule-delivery technology has progressed over the last decades, with the development of sustained release systems for application in food sector. It is useful to control the rate and extent of food digestion in different regions of the gastrointestinal tract (GIT) and release of an active compound with the ultimate goal of controlling bioavailability and satiety of blood levels. The nature, molecular weight and concentration of a carrier material, as well as the lipophilic/hydrophilic character of the loaded active component, its concentration, interfacial properties and the particle size distribution are some of the key factors that determine the delivery performance of these systems. pH variation, temperature change, variation of the redox potential and enzyme activity are stimuli which most often induce the release of an active molecule in a medium. In order to develop food additives with controlled release, their processing in the gastrointestinal tract has to be understood; therefore, a general description of the digestive system is given and associated to the currently used in vitro experimental models.

This chapter aims to give a useful overview on the gastrointestinal fate of the most frequently used carrier materials and structures of food-grade delivery systems. At the end, mathematical models most useful in fitting to the experimental data of release profiles and resolving the mechanisms implied in controlled release are summarized.",
publisher = "CRC Press",
journal = "Encapsulation in Food Processing and Fermentation",
booktitle = "Controlled Release Systems for Food Application",
pages = "171-142",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6128"
}

Đorđević, V., Balanč, B., Kalušević, A., Volić, M., Obradović, N., Petrović, P.,& Bugarski, B.. (2022). Controlled Release Systems for Food Application. in Encapsulation in Food Processing and Fermentation
CRC Press., 142-171.
https://hdl.handle.net/21.15107/rcub_technorep_6128

Đorđević V, Balanč B, Kalušević A, Volić M, Obradović N, Petrović P, Bugarski B. Controlled Release Systems for Food Application. in Encapsulation in Food Processing and Fermentation. 2022;:142-171.
https://hdl.handle.net/21.15107/rcub_technorep_6128 .

Đorđević, Verica, Balanč, Bojana, Kalušević, Ana, Volić, Mina, Obradović, Nataša, Petrović, Predrag, Bugarski, Branko, "Controlled Release Systems for Food Application" in Encapsulation in Food Processing and Fermentation (2022):142-171,
https://hdl.handle.net/21.15107/rcub_technorep_6128 .

TY  - CONF
AU  - Obradović, Nataša
AU  - Volić, Mina
AU  - Jovanović, Aleksandra
AU  - Krunić, Tanja
AU  - Nedović, Viktor
AU  - Rakin, Marica
AU  - Bugarski, Branko
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6446
AB  - Starter cultures are essential components of fermented foods with the potential to enhance
preservation, improve nutritional quality, and modify the sensory qualities of the products. The
study characterized the microcarriers with the probiotic starter culture which could be used for
the preparation of functional whey-based products. The culture was encapsulated using the
spray-drying technique and implemented in the whey-based medium. The protective effect of
alginate-whey carriers on the microencapsulated dairy starter culture (Lactoferm ABY 6)
during the spray-drying, fermentation, and storage time were analyzed. The production yield,
encapsulation efficiency, solubility, particle size, and moisture content were determined after
the microencapsulation process. The SEM analysis was used for the characterization of powder
and carrier surface morphology. The encapsulation efficiency and powder solubility were
75,8±0,09 % and 86,0±1,62 %, respectively. The cell viability in the powders and beverages
was monitored for 28 days. The results showed that the microencapsulation process improved
cell viability (> 9,08 log10CFU/mL) during the fermentation process. Moreover,
microencapsulated probiotic cells have a higher survival rate in beverages in comparison with
free cells in storage time. The application of this type of encapsulation technique showed
potential regarding cell preservation in the production and storage of functional beverages.
PB  - Novi Sad : Faculty of Technology
C3  - Book of abstracts / 2nd International Conference on Advanced Production and Processing, 20th-22nd October 2022, Novi Sad
T1  - Design and characterization of spray-dried microcarriers for preparation of functional wheybased beverages
SP  - 35
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6446
ER  - 

@conference{
author = "Obradović, Nataša and Volić, Mina and Jovanović, Aleksandra and Krunić, Tanja and Nedović, Viktor and Rakin, Marica and Bugarski, Branko",
year = "2022",
abstract = "Starter cultures are essential components of fermented foods with the potential to enhance
preservation, improve nutritional quality, and modify the sensory qualities of the products. The
study characterized the microcarriers with the probiotic starter culture which could be used for
the preparation of functional whey-based products. The culture was encapsulated using the
spray-drying technique and implemented in the whey-based medium. The protective effect of
alginate-whey carriers on the microencapsulated dairy starter culture (Lactoferm ABY 6)
during the spray-drying, fermentation, and storage time were analyzed. The production yield,
encapsulation efficiency, solubility, particle size, and moisture content were determined after
the microencapsulation process. The SEM analysis was used for the characterization of powder
and carrier surface morphology. The encapsulation efficiency and powder solubility were
75,8±0,09 % and 86,0±1,62 %, respectively. The cell viability in the powders and beverages
was monitored for 28 days. The results showed that the microencapsulation process improved
cell viability (> 9,08 log10CFU/mL) during the fermentation process. Moreover,
microencapsulated probiotic cells have a higher survival rate in beverages in comparison with
free cells in storage time. The application of this type of encapsulation technique showed
potential regarding cell preservation in the production and storage of functional beverages.",
publisher = "Novi Sad : Faculty of Technology",
journal = "Book of abstracts / 2nd International Conference on Advanced Production and Processing, 20th-22nd October 2022, Novi Sad",
title = "Design and characterization of spray-dried microcarriers for preparation of functional wheybased beverages",
pages = "35",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6446"
}

Obradović, N., Volić, M., Jovanović, A., Krunić, T., Nedović, V., Rakin, M.,& Bugarski, B.. (2022). Design and characterization of spray-dried microcarriers for preparation of functional wheybased beverages. in Book of abstracts / 2nd International Conference on Advanced Production and Processing, 20th-22nd October 2022, Novi Sad
Novi Sad : Faculty of Technology., 35.
https://hdl.handle.net/21.15107/rcub_technorep_6446

Obradović N, Volić M, Jovanović A, Krunić T, Nedović V, Rakin M, Bugarski B. Design and characterization of spray-dried microcarriers for preparation of functional wheybased beverages. in Book of abstracts / 2nd International Conference on Advanced Production and Processing, 20th-22nd October 2022, Novi Sad. 2022;:35.
https://hdl.handle.net/21.15107/rcub_technorep_6446 .

Obradović, Nataša, Volić, Mina, Jovanović, Aleksandra, Krunić, Tanja, Nedović, Viktor, Rakin, Marica, Bugarski, Branko, "Design and characterization of spray-dried microcarriers for preparation of functional wheybased beverages" in Book of abstracts / 2nd International Conference on Advanced Production and Processing, 20th-22nd October 2022, Novi Sad (2022):35,
https://hdl.handle.net/21.15107/rcub_technorep_6446 .

TY  - JOUR
AU  - Obradović, Nataša
AU  - Volić, Mina
AU  - Nedović, Viktor
AU  - Rakin, Marica
AU  - Bugarski, Branko
PY  - 2022
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6031
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5035
AB  - The production of functional beverages has gained more attention because of growing consumers' interest in personal health. The process of carrier production and characterization of a novel type of protein-carbohydrate carriers for functional beverages with a focus on cell protection during fermentation, gastrointestinal, and storage conditions are shown in this study. Probiotic starter culture was microencapsulated using spray and freeze-drying techniques and then implemented in whey-based beverages. Encapsulation materials used are whey protein concentrate, whey, and sodium alginate. The encapsulation efficiency results were over 76.8% and 81.4%, after spray and freeze-drying respectively, depending on the carrier formulation. The culture in freeze-dried carriers reached a higher survival rate (>7.5 log10(CFUg−1)) than one's encapsulated by spray-drying technique during storage. Microencapsulation of the culture significantly improved cell viability in gastrointestinal and storage conditions compared with free cells. Usage of both encapsulation techniques showed potential regarding cell preservation in production and storage.
PB  - Elsevier Ltd.
T2  - Journal of Food Engineering
T1  - Microencapsulation of probiotic starter culture in protein–carbohydrate carriers using spray and freeze-drying processes: Implementation in whey-based beverages
SP  - 110948
VL  - 321
DO  - 10.1016/j.jfoodeng.2022.110948
ER  - 

@article{
author = "Obradović, Nataša and Volić, Mina and Nedović, Viktor and Rakin, Marica and Bugarski, Branko",
year = "2022",
abstract = "The production of functional beverages has gained more attention because of growing consumers' interest in personal health. The process of carrier production and characterization of a novel type of protein-carbohydrate carriers for functional beverages with a focus on cell protection during fermentation, gastrointestinal, and storage conditions are shown in this study. Probiotic starter culture was microencapsulated using spray and freeze-drying techniques and then implemented in whey-based beverages. Encapsulation materials used are whey protein concentrate, whey, and sodium alginate. The encapsulation efficiency results were over 76.8% and 81.4%, after spray and freeze-drying respectively, depending on the carrier formulation. The culture in freeze-dried carriers reached a higher survival rate (>7.5 log10(CFUg−1)) than one's encapsulated by spray-drying technique during storage. Microencapsulation of the culture significantly improved cell viability in gastrointestinal and storage conditions compared with free cells. Usage of both encapsulation techniques showed potential regarding cell preservation in production and storage.",
publisher = "Elsevier Ltd.",
journal = "Journal of Food Engineering",
title = "Microencapsulation of probiotic starter culture in protein–carbohydrate carriers using spray and freeze-drying processes: Implementation in whey-based beverages",
pages = "110948",
volume = "321",
doi = "10.1016/j.jfoodeng.2022.110948"
}

Obradović, N., Volić, M., Nedović, V., Rakin, M.,& Bugarski, B.. (2022). Microencapsulation of probiotic starter culture in protein–carbohydrate carriers using spray and freeze-drying processes: Implementation in whey-based beverages. in Journal of Food Engineering
Elsevier Ltd.., 321, 110948.
https://doi.org/10.1016/j.jfoodeng.2022.110948

Obradović N, Volić M, Nedović V, Rakin M, Bugarski B. Microencapsulation of probiotic starter culture in protein–carbohydrate carriers using spray and freeze-drying processes: Implementation in whey-based beverages. in Journal of Food Engineering. 2022;321:110948.
doi:10.1016/j.jfoodeng.2022.110948 .

Obradović, Nataša, Volić, Mina, Nedović, Viktor, Rakin, Marica, Bugarski, Branko, "Microencapsulation of probiotic starter culture in protein–carbohydrate carriers using spray and freeze-drying processes: Implementation in whey-based beverages" in Journal of Food Engineering, 321 (2022):110948,
https://doi.org/10.1016/j.jfoodeng.2022.110948 . .

TY  - JOUR
AU  - Volić, Mina
AU  - Pećinar, Ilinka
AU  - Micić, Darko
AU  - Đorđević, Verica
AU  - Pešić, Radojica
AU  - Nedović, Viktor
AU  - Obradović, Nataša
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5067
AB  - Innovative coating powders, based on whey protein concentrate (10–15 wt%) as native (WPC) or denatured protein (D-WPC), solely or in combination with alginate (0.75 wt%, AL), containing thyme essential oil, were produced using the freeze-drying technique. The impact of individual components (protein, alginate and oil) as well as the effect of heat-induced protein denaturation, was resolved regarding physicochemical, thermal and morphological properties of powders. High product yield (∼100%), particle size (223–257 nm), low moisture content (0.10–0.13%) and zeta potential (−19 to −25.6 mV) were determined for all samples. Strong antimicrobial activity of thyme oil nanocarriers against foodborne pathogens was demonstrated. Thermogravimetric analysis (TGA) indicated enhanced thermal stability of encapsulated oil. The most specific bands of structural compounds were identified in Raman spectra of the tested formulations, but principal component analysis (PCA) on recorded spectra was necessary to show the differences between carriers of different wall materials.
PB  - Elsevier
T2  - Food Chemistry
T1  - Design and characterization of whey protein nanocarriers for thyme essential oil encapsulation obtained by freeze-drying
SP  - 132749
VL  - 386
DO  - 10.1016/j.foodchem.2022.132749
ER  - 

@article{
author = "Volić, Mina and Pećinar, Ilinka and Micić, Darko and Đorđević, Verica and Pešić, Radojica and Nedović, Viktor and Obradović, Nataša",
year = "2022",
abstract = "Innovative coating powders, based on whey protein concentrate (10–15 wt%) as native (WPC) or denatured protein (D-WPC), solely or in combination with alginate (0.75 wt%, AL), containing thyme essential oil, were produced using the freeze-drying technique. The impact of individual components (protein, alginate and oil) as well as the effect of heat-induced protein denaturation, was resolved regarding physicochemical, thermal and morphological properties of powders. High product yield (∼100%), particle size (223–257 nm), low moisture content (0.10–0.13%) and zeta potential (−19 to −25.6 mV) were determined for all samples. Strong antimicrobial activity of thyme oil nanocarriers against foodborne pathogens was demonstrated. Thermogravimetric analysis (TGA) indicated enhanced thermal stability of encapsulated oil. The most specific bands of structural compounds were identified in Raman spectra of the tested formulations, but principal component analysis (PCA) on recorded spectra was necessary to show the differences between carriers of different wall materials.",
publisher = "Elsevier",
journal = "Food Chemistry",
title = "Design and characterization of whey protein nanocarriers for thyme essential oil encapsulation obtained by freeze-drying",
pages = "132749",
volume = "386",
doi = "10.1016/j.foodchem.2022.132749"
}

Volić, M., Pećinar, I., Micić, D., Đorđević, V., Pešić, R., Nedović, V.,& Obradović, N.. (2022). Design and characterization of whey protein nanocarriers for thyme essential oil encapsulation obtained by freeze-drying. in Food Chemistry
Elsevier., 386, 132749.
https://doi.org/10.1016/j.foodchem.2022.132749

Volić M, Pećinar I, Micić D, Đorđević V, Pešić R, Nedović V, Obradović N. Design and characterization of whey protein nanocarriers for thyme essential oil encapsulation obtained by freeze-drying. in Food Chemistry. 2022;386:132749.
doi:10.1016/j.foodchem.2022.132749 .

Volić, Mina, Pećinar, Ilinka, Micić, Darko, Đorđević, Verica, Pešić, Radojica, Nedović, Viktor, Obradović, Nataša, "Design and characterization of whey protein nanocarriers for thyme essential oil encapsulation obtained by freeze-drying" in Food Chemistry, 386 (2022):132749,
https://doi.org/10.1016/j.foodchem.2022.132749 . .

TY  - CONF
AU  - Volić, Mina
AU  - Obradović, Nataša
AU  - Jovanović, Aleksandra
AU  - Bugarski, Branko
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6451
AB  - Cherry fruits are known for their wide range of nutritive and bioactive compounds, including
fibers, vitamins, and phytonutrients. Sweet cherry consumption has been associated with
several health benefits against human problems such as cardiovascular diseases, cancer,
diabetes, and Alzheimer’s disease. Particularly, cherries contain abundant phenolic compounds
with antioxidant, anti-inflammatory, and anticancer properties as they exert a protective role
against oxidative stress and free radical damages. However, there is not a specific method to
fully extract all phenolics and other compounds from sweet cherries. Thus, this study aimed to
optimize the extraction of cherries through different extraction procedures (maceration and
ultrasound-assisted extraction) using different solvents (50% EtOH with and without HCl, and
Tris buffer). The resulting cherry fruit extracts were investigated regarding their i) total phenol
content - according to the Folin-Ciocalteu colorimetric method, ii) total protein content -
evaluated using the Bradford protein assay, and iii) antioxidant activity – characterized by
ABTS, DPPH, and FRAP assays. The ultrasound-assisted extraction using 50% EtOH and HCl
(pH=2.3) demonstrated the best results for total phenol content (7.51 mg/L), DPPH inhibition
(23.7%), and FRAP reducing power (24.5 mmol/g). On the contrary, the highest ABTS
scavenging activity was noticed for maceration obtained extract with 50% EtOH and HCl (7.23
mg/mL). Finally, the best protein yield was obtained for cherries when extraction was
performed in 0.1M Tris buffer at pH=11.2 (0.23 mg/mL). The optimization methodology in this
work is used as a screening test for producing bioactive compounds-rich extracts for the food
industry
PB  - University of Novi Sad, Faculty of Technology Novi Sad
C3  - Book of Abstracts of the 2nd International Conference on Advanced Production and Processing
T1  - Optimization of Sweet Cherry Extraction: Evaluation of Total Phenol Content, Protein Content, and Antioxidant Activity of Extracts
SP  - 180
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6451
ER  - 

@conference{
author = "Volić, Mina and Obradović, Nataša and Jovanović, Aleksandra and Bugarski, Branko",
year = "2022",
abstract = "Cherry fruits are known for their wide range of nutritive and bioactive compounds, including
fibers, vitamins, and phytonutrients. Sweet cherry consumption has been associated with
several health benefits against human problems such as cardiovascular diseases, cancer,
diabetes, and Alzheimer’s disease. Particularly, cherries contain abundant phenolic compounds
with antioxidant, anti-inflammatory, and anticancer properties as they exert a protective role
against oxidative stress and free radical damages. However, there is not a specific method to
fully extract all phenolics and other compounds from sweet cherries. Thus, this study aimed to
optimize the extraction of cherries through different extraction procedures (maceration and
ultrasound-assisted extraction) using different solvents (50% EtOH with and without HCl, and
Tris buffer). The resulting cherry fruit extracts were investigated regarding their i) total phenol
content - according to the Folin-Ciocalteu colorimetric method, ii) total protein content -
evaluated using the Bradford protein assay, and iii) antioxidant activity – characterized by
ABTS, DPPH, and FRAP assays. The ultrasound-assisted extraction using 50% EtOH and HCl
(pH=2.3) demonstrated the best results for total phenol content (7.51 mg/L), DPPH inhibition
(23.7%), and FRAP reducing power (24.5 mmol/g). On the contrary, the highest ABTS
scavenging activity was noticed for maceration obtained extract with 50% EtOH and HCl (7.23
mg/mL). Finally, the best protein yield was obtained for cherries when extraction was
performed in 0.1M Tris buffer at pH=11.2 (0.23 mg/mL). The optimization methodology in this
work is used as a screening test for producing bioactive compounds-rich extracts for the food
industry",
publisher = "University of Novi Sad, Faculty of Technology Novi Sad",
journal = "Book of Abstracts of the 2nd International Conference on Advanced Production and Processing",
title = "Optimization of Sweet Cherry Extraction: Evaluation of Total Phenol Content, Protein Content, and Antioxidant Activity of Extracts",
pages = "180",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6451"
}

Volić, M., Obradović, N., Jovanović, A.,& Bugarski, B.. (2022). Optimization of Sweet Cherry Extraction: Evaluation of Total Phenol Content, Protein Content, and Antioxidant Activity of Extracts. in Book of Abstracts of the 2nd International Conference on Advanced Production and Processing
University of Novi Sad, Faculty of Technology Novi Sad., 180.
https://hdl.handle.net/21.15107/rcub_technorep_6451

Volić M, Obradović N, Jovanović A, Bugarski B. Optimization of Sweet Cherry Extraction: Evaluation of Total Phenol Content, Protein Content, and Antioxidant Activity of Extracts. in Book of Abstracts of the 2nd International Conference on Advanced Production and Processing. 2022;:180.
https://hdl.handle.net/21.15107/rcub_technorep_6451 .

Volić, Mina, Obradović, Nataša, Jovanović, Aleksandra, Bugarski, Branko, "Optimization of Sweet Cherry Extraction: Evaluation of Total Phenol Content, Protein Content, and Antioxidant Activity of Extracts" in Book of Abstracts of the 2nd International Conference on Advanced Production and Processing (2022):180,
https://hdl.handle.net/21.15107/rcub_technorep_6451 .

TY  - JOUR
AU  - Volić, Mina M.
AU  - Đorđević, Verica B.
AU  - Vukašinović-Sekulić, Maja
AU  - Obradović, Nataša S.
AU  - Knežević-Jugović, Zorica D.
AU  - Bugarski, Branko M.
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5916
AB  - The aim of this study was to develop a stable hydrogel carrier system for thyme essential oil (TEO) that could protect its sensitive polyphenol compounds. The impact of wall material (soy protein and alginate) on encapsulation efficiency and thymol release in simulated gastrointestinal conditions, was investigated. The release of thymol was ~ 80 % and 20 % in simulated gastric and pancreatic solutions, respectively. Thyme essential oil plays an important role as an antimicrobial and antioxidant agent. Results indicated that encapsulated TEO inside the hydrogel matrix exhibited antioxidant activity demonstrated by CUPRAC and ABTS analysis, even after thermal treatment of the beads, indicating the metal chelate effect as dominant. In vitro antimicrobial activity of encapsulated TEO has been studied against several pathogenic microorganisms such as Escherichia coli, Staphylococcus aureus, Bacillus cereus and Candida albicans. Beads coded as Ca-A1.5/SP1.5 showed anti-Candida albicans activity, while modified bead formulations Ca-A1.5/SP1.5* and Ca-A1.5/SP0.25** showed bactericidal activity against Escherichia coli and Staphylococcus aureus.
PB  - University of Novi Sad, Faculty of Technology
T2  - Acta Periodica Technologica
T1  - ANTIOXIDANT and ANTIMICROBIAL CAPACITY of ENCAPSULATED THYME ESSENTIAL OIL in ALGINATE and SOY PROTEIN-BASED CARRIERS
EP  - 12
IS  - 52
SP  - 1
DO  - 10.2298/APT2152001V
ER  - 

@article{
author = "Volić, Mina M. and Đorđević, Verica B. and Vukašinović-Sekulić, Maja and Obradović, Nataša S. and Knežević-Jugović, Zorica D. and Bugarski, Branko M.",
year = "2021",
abstract = "The aim of this study was to develop a stable hydrogel carrier system for thyme essential oil (TEO) that could protect its sensitive polyphenol compounds. The impact of wall material (soy protein and alginate) on encapsulation efficiency and thymol release in simulated gastrointestinal conditions, was investigated. The release of thymol was ~ 80 % and 20 % in simulated gastric and pancreatic solutions, respectively. Thyme essential oil plays an important role as an antimicrobial and antioxidant agent. Results indicated that encapsulated TEO inside the hydrogel matrix exhibited antioxidant activity demonstrated by CUPRAC and ABTS analysis, even after thermal treatment of the beads, indicating the metal chelate effect as dominant. In vitro antimicrobial activity of encapsulated TEO has been studied against several pathogenic microorganisms such as Escherichia coli, Staphylococcus aureus, Bacillus cereus and Candida albicans. Beads coded as Ca-A1.5/SP1.5 showed anti-Candida albicans activity, while modified bead formulations Ca-A1.5/SP1.5* and Ca-A1.5/SP0.25** showed bactericidal activity against Escherichia coli and Staphylococcus aureus.",
publisher = "University of Novi Sad, Faculty of Technology",
journal = "Acta Periodica Technologica",
title = "ANTIOXIDANT and ANTIMICROBIAL CAPACITY of ENCAPSULATED THYME ESSENTIAL OIL in ALGINATE and SOY PROTEIN-BASED CARRIERS",
pages = "12-1",
number = "52",
doi = "10.2298/APT2152001V"
}

Volić, M. M., Đorđević, V. B., Vukašinović-Sekulić, M., Obradović, N. S., Knežević-Jugović, Z. D.,& Bugarski, B. M.. (2021). ANTIOXIDANT and ANTIMICROBIAL CAPACITY of ENCAPSULATED THYME ESSENTIAL OIL in ALGINATE and SOY PROTEIN-BASED CARRIERS. in Acta Periodica Technologica
University of Novi Sad, Faculty of Technology.(52), 1-12.
https://doi.org/10.2298/APT2152001V

Volić MM, Đorđević VB, Vukašinović-Sekulić M, Obradović NS, Knežević-Jugović ZD, Bugarski BM. ANTIOXIDANT and ANTIMICROBIAL CAPACITY of ENCAPSULATED THYME ESSENTIAL OIL in ALGINATE and SOY PROTEIN-BASED CARRIERS. in Acta Periodica Technologica. 2021;(52):1-12.
doi:10.2298/APT2152001V .

Volić, Mina M., Đorđević, Verica B., Vukašinović-Sekulić, Maja, Obradović, Nataša S., Knežević-Jugović, Zorica D., Bugarski, Branko M., "ANTIOXIDANT and ANTIMICROBIAL CAPACITY of ENCAPSULATED THYME ESSENTIAL OIL in ALGINATE and SOY PROTEIN-BASED CARRIERS" in Acta Periodica Technologica, no. 52 (2021):1-12,
https://doi.org/10.2298/APT2152001V . .

TY  - CONF
AU  - Galić, Aleksa
AU  - Matić, Tamara
AU  - Obradović, Nataša
AU  - Veljović, Đorđe
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6675
AB  - Autologous and allogenic grafts are often referred to as the gold standard for bone tissue
transplants, but possess drawbacks such as risk of immunological reaction and infection,
limited bone bank capacity and pain for the patient, which is why synthetic transplants are a
highly researched topic.
PB  - Belgrade : Institute of Technical Sciences of SASA
C3  - Program and the Book of abstracts / Nineteenth Young Researchers' Conference Materials Science and Engineering, December 1-3, 2021, Belgrade, Serbia
T1  - Processing of gelatine coated composite scaffolds based on magnesium and strontium doped hydroxyapatite and yttria-stabilised zicronium oxide
SP  - 15
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6675
ER  - 

@conference{
author = "Galić, Aleksa and Matić, Tamara and Obradović, Nataša and Veljović, Đorđe",
year = "2021",
abstract = "Autologous and allogenic grafts are often referred to as the gold standard for bone tissue
transplants, but possess drawbacks such as risk of immunological reaction and infection,
limited bone bank capacity and pain for the patient, which is why synthetic transplants are a
highly researched topic.",
publisher = "Belgrade : Institute of Technical Sciences of SASA",
journal = "Program and the Book of abstracts / Nineteenth Young Researchers' Conference Materials Science and Engineering, December 1-3, 2021, Belgrade, Serbia",
title = "Processing of gelatine coated composite scaffolds based on magnesium and strontium doped hydroxyapatite and yttria-stabilised zicronium oxide",
pages = "15",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6675"
}

Galić, A., Matić, T., Obradović, N.,& Veljović, Đ.. (2021). Processing of gelatine coated composite scaffolds based on magnesium and strontium doped hydroxyapatite and yttria-stabilised zicronium oxide. in Program and the Book of abstracts / Nineteenth Young Researchers' Conference Materials Science and Engineering, December 1-3, 2021, Belgrade, Serbia
Belgrade : Institute of Technical Sciences of SASA., 15.
https://hdl.handle.net/21.15107/rcub_technorep_6675

Galić A, Matić T, Obradović N, Veljović Đ. Processing of gelatine coated composite scaffolds based on magnesium and strontium doped hydroxyapatite and yttria-stabilised zicronium oxide. in Program and the Book of abstracts / Nineteenth Young Researchers' Conference Materials Science and Engineering, December 1-3, 2021, Belgrade, Serbia. 2021;:15.
https://hdl.handle.net/21.15107/rcub_technorep_6675 .

Galić, Aleksa, Matić, Tamara, Obradović, Nataša, Veljović, Đorđe, "Processing of gelatine coated composite scaffolds based on magnesium and strontium doped hydroxyapatite and yttria-stabilised zicronium oxide" in Program and the Book of abstracts / Nineteenth Young Researchers' Conference Materials Science and Engineering, December 1-3, 2021, Belgrade, Serbia (2021):15,
https://hdl.handle.net/21.15107/rcub_technorep_6675 .

TY  - JOUR
AU  - Turkovic, Erna
AU  - Vasiljevic, Ivana
AU  - Draskovic, Milica
AU  - Obradović, Nataša
AU  - Vasiljevic, Dragana
AU  - Parojcic, Jelena
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4881
AB  - Inkjet printing is novel approach in drug manufacturing that enables dispensing precise volumes of ink onto substrates. Optimal substrate properties including suitable mechanical characteristic are recognized as crucial to achieve desired dosage form performance upon administration. Identification of relevant quality attributes and their quantification is subject of intensive scientific research. The aim of this work was to explore applicability of different materials as printing substrates and explore contribution of the investigated substrate properties to its printability. Substrates were characterized with regards to uniformity, porosity, disintegration time, mechanical properties and drug dissolution. Experimentally obtained values were mathematically transformed and the obtained results were presented as relevant radar charts. It was shown that structurally different substrates may be employed for orodispersible films inkjet printing. Main disadvantage of single-polymer films was low drug load, and their printability was dependent on film flexibility and mechanical strength. Structured orodispersible film templates exhibited favorable mechanical properties and drug load capacity. Wafer edible sheets were characterized with high mechanical resistance and brittleness which somewhat diminished printability, but did not hinder high drug load. Obtained results provide insight into application of different materials as printing substrates and contribute to understanding of substrate properties which can affect printability.
T2  - Pharmaceutics
T1  - An Investigation into Mechanical Properties and Printability of Potential Substrates for Inkjet Printing of Orodispersible Films
IS  - 4
VL  - 13
DO  - 10.3390/pharmaceutics13040468
ER  - 

@article{
author = "Turkovic, Erna and Vasiljevic, Ivana and Draskovic, Milica and Obradović, Nataša and Vasiljevic, Dragana and Parojcic, Jelena",
year = "2021",
abstract = "Inkjet printing is novel approach in drug manufacturing that enables dispensing precise volumes of ink onto substrates. Optimal substrate properties including suitable mechanical characteristic are recognized as crucial to achieve desired dosage form performance upon administration. Identification of relevant quality attributes and their quantification is subject of intensive scientific research. The aim of this work was to explore applicability of different materials as printing substrates and explore contribution of the investigated substrate properties to its printability. Substrates were characterized with regards to uniformity, porosity, disintegration time, mechanical properties and drug dissolution. Experimentally obtained values were mathematically transformed and the obtained results were presented as relevant radar charts. It was shown that structurally different substrates may be employed for orodispersible films inkjet printing. Main disadvantage of single-polymer films was low drug load, and their printability was dependent on film flexibility and mechanical strength. Structured orodispersible film templates exhibited favorable mechanical properties and drug load capacity. Wafer edible sheets were characterized with high mechanical resistance and brittleness which somewhat diminished printability, but did not hinder high drug load. Obtained results provide insight into application of different materials as printing substrates and contribute to understanding of substrate properties which can affect printability.",
journal = "Pharmaceutics",
title = "An Investigation into Mechanical Properties and Printability of Potential Substrates for Inkjet Printing of Orodispersible Films",
number = "4",
volume = "13",
doi = "10.3390/pharmaceutics13040468"
}

Turkovic, E., Vasiljevic, I., Draskovic, M., Obradović, N., Vasiljevic, D.,& Parojcic, J.. (2021). An Investigation into Mechanical Properties and Printability of Potential Substrates for Inkjet Printing of Orodispersible Films. in Pharmaceutics, 13(4).
https://doi.org/10.3390/pharmaceutics13040468

Turkovic E, Vasiljevic I, Draskovic M, Obradović N, Vasiljevic D, Parojcic J. An Investigation into Mechanical Properties and Printability of Potential Substrates for Inkjet Printing of Orodispersible Films. in Pharmaceutics. 2021;13(4).
doi:10.3390/pharmaceutics13040468 .

Turkovic, Erna, Vasiljevic, Ivana, Draskovic, Milica, Obradović, Nataša, Vasiljevic, Dragana, Parojcic, Jelena, "An Investigation into Mechanical Properties and Printability of Potential Substrates for Inkjet Printing of Orodispersible Films" in Pharmaceutics, 13, no. 4 (2021),
https://doi.org/10.3390/pharmaceutics13040468 . .

TY  - JOUR
AU  - Obradović, Nataša
AU  - Pajić-Lijaković, Ivana
AU  - Krunić, Tanja
AU  - Belović, Miona
AU  - Rakin, Marica
AU  - Bugarski, Branko
PY  - 2020
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4463
AB  - The suitability of natural hydrogel carriers with probiotic starter culture as whey beverages supplements was examined by assessing their rheological and structural changes during the fermentation and gastrointestinal conditions. Effect of encapsulated cells on the carrier structure is of great importance for the selection of proper material for the preparation of functional supplements. The structural changes of the chitosan-coated alginate/whey carriers were considered based on (1) cell viability and the carrier average volume vs. time (2) the storage and loss modulus vs. time obtained under low oscillator strain conditions, (3) FTIR analysis and (4) SEM cross-sectional observation of the hydrogel carriers. The presence of chitosan coating and fermentation conditions increased cell viability up to 9.01 +/- 0.18 (log CFU/g). According to our results, the encapsulated cells induce weakening of carriers under the gastric conditions but improve their mechanical stability under the intestinal condition. The mechanical behaviour of carriers was also considered in order to formulate the rheological constitutive model equation for describing the irreversible structural changes under the gastric and intestinal conditions. The cell leakage under the gastric condition after the 2 h was less than 5%. Carriers are rapidly degraded under the intestinal condition which ensures the release of cells and provides their beneficial effects on the host health. Our results indicate that this type of coated carrier is suitable to be used for encapsulation of probiotic starter culture in the production of fermented whey-based products.
PB  - Springer, New York
T2  - Food Biophysics
T1  - Effect of Encapsulated Probiotic Starter Culture on Rheological and Structural Properties of Natural Hydrogel Carriers Affected by Fermentation and Gastrointestinal Conditions
EP  - 31
IS  - 1
SP  - 18
VL  - 15
DO  - 10.1007/s11483-019-09598-8
ER  - 

@article{
author = "Obradović, Nataša and Pajić-Lijaković, Ivana and Krunić, Tanja and Belović, Miona and Rakin, Marica and Bugarski, Branko",
year = "2020",
abstract = "The suitability of natural hydrogel carriers with probiotic starter culture as whey beverages supplements was examined by assessing their rheological and structural changes during the fermentation and gastrointestinal conditions. Effect of encapsulated cells on the carrier structure is of great importance for the selection of proper material for the preparation of functional supplements. The structural changes of the chitosan-coated alginate/whey carriers were considered based on (1) cell viability and the carrier average volume vs. time (2) the storage and loss modulus vs. time obtained under low oscillator strain conditions, (3) FTIR analysis and (4) SEM cross-sectional observation of the hydrogel carriers. The presence of chitosan coating and fermentation conditions increased cell viability up to 9.01 +/- 0.18 (log CFU/g). According to our results, the encapsulated cells induce weakening of carriers under the gastric conditions but improve their mechanical stability under the intestinal condition. The mechanical behaviour of carriers was also considered in order to formulate the rheological constitutive model equation for describing the irreversible structural changes under the gastric and intestinal conditions. The cell leakage under the gastric condition after the 2 h was less than 5%. Carriers are rapidly degraded under the intestinal condition which ensures the release of cells and provides their beneficial effects on the host health. Our results indicate that this type of coated carrier is suitable to be used for encapsulation of probiotic starter culture in the production of fermented whey-based products.",
publisher = "Springer, New York",
journal = "Food Biophysics",
title = "Effect of Encapsulated Probiotic Starter Culture on Rheological and Structural Properties of Natural Hydrogel Carriers Affected by Fermentation and Gastrointestinal Conditions",
pages = "31-18",
number = "1",
volume = "15",
doi = "10.1007/s11483-019-09598-8"
}

Obradović, N., Pajić-Lijaković, I., Krunić, T., Belović, M., Rakin, M.,& Bugarski, B.. (2020). Effect of Encapsulated Probiotic Starter Culture on Rheological and Structural Properties of Natural Hydrogel Carriers Affected by Fermentation and Gastrointestinal Conditions. in Food Biophysics
Springer, New York., 15(1), 18-31.
https://doi.org/10.1007/s11483-019-09598-8

Obradović N, Pajić-Lijaković I, Krunić T, Belović M, Rakin M, Bugarski B. Effect of Encapsulated Probiotic Starter Culture on Rheological and Structural Properties of Natural Hydrogel Carriers Affected by Fermentation and Gastrointestinal Conditions. in Food Biophysics. 2020;15(1):18-31.
doi:10.1007/s11483-019-09598-8 .

Obradović, Nataša, Pajić-Lijaković, Ivana, Krunić, Tanja, Belović, Miona, Rakin, Marica, Bugarski, Branko, "Effect of Encapsulated Probiotic Starter Culture on Rheological and Structural Properties of Natural Hydrogel Carriers Affected by Fermentation and Gastrointestinal Conditions" in Food Biophysics, 15, no. 1 (2020):18-31,
https://doi.org/10.1007/s11483-019-09598-8 . .

TY  - JOUR
AU  - Dajić-Stevanović, Zora
AU  - Sieniawska, Elwira
AU  - Glowniak, Kazimierz
AU  - Obradović, Nataša
AU  - Pajić-Lijaković, Ivana
PY  - 2020
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4517
AB  - Essential oils (EOs) and their main constituents, the terpenes, are widely studied, mostly relating to their antioxidant ability and bioactivity, such as antimicrobial, anticancer, anti-inflammatory, and range of other actions in the living systems. However, there is limited information on their bioavailability, especially upon clinical studies. Having in mind both strong biological effects and health benefits of EOs and their specific physicochemical properties (volatility, lipophilic character, low water solubility or insolubility, viscosity, expressed odor, concentration-dependent toxicity, etc.), there is a need for their encapsulation for target delivery. Encapsulation of EOs and their constituents is the prerequisite for enhancing their oxidative stability, thermostability, photostability, shelf life, and biological activity. We considered various carrier types such a (1) monophase and polyphase polysaccharide hydrogel carriers, (2) polysaccharide-protein carriers, and (3) lipid carriers in the context of physicochemical and engineering factors. Physicochemical factors are encapsulation efficiency, chemical stability under gastric conditions, mechanical stability, and thermal stability of carrier matrices. Choice of carrier material also determines the encapsulation technique. Consequently, the engineering factors are related to the advantage and disadvantage of various encapsulation techniques frequently used in the literature. In addition, it was intended to address the interactions between (1) main carrier components, such as polysaccharides, proteins, and lipids themselves (in order to form chemically and mechanically stable structure); (2) main carrier components with pepsin under gastric conditions (in order to form resistant material under gastric conditions); and (3) main carrier components with EOs (in order to enhance encapsulation efficiency), as a necessary precondition for whole process optimization. Finally, different sources for obtaining natural carrier macromolecules are surveyed, especially the agro-waste materials and agricultural and food by-products. This review article highlights the bioavailability aspects of encapsulated EOs and physicochemical and engineering factors concerning natural macromolecule carriers for their target delivery and application.
PB  - Frontiers Media Sa, Lausanne
T2  - Frontiers in Bioengineering and Biotechnology
T1  - Natural Macromolecules as Carriers for Essential Oils: From Extraction to Biomedical Application
VL  - 8
DO  - 10.3389/fbioe.2020.00563
ER  - 

@article{
author = "Dajić-Stevanović, Zora and Sieniawska, Elwira and Glowniak, Kazimierz and Obradović, Nataša and Pajić-Lijaković, Ivana",
year = "2020",
abstract = "Essential oils (EOs) and their main constituents, the terpenes, are widely studied, mostly relating to their antioxidant ability and bioactivity, such as antimicrobial, anticancer, anti-inflammatory, and range of other actions in the living systems. However, there is limited information on their bioavailability, especially upon clinical studies. Having in mind both strong biological effects and health benefits of EOs and their specific physicochemical properties (volatility, lipophilic character, low water solubility or insolubility, viscosity, expressed odor, concentration-dependent toxicity, etc.), there is a need for their encapsulation for target delivery. Encapsulation of EOs and their constituents is the prerequisite for enhancing their oxidative stability, thermostability, photostability, shelf life, and biological activity. We considered various carrier types such a (1) monophase and polyphase polysaccharide hydrogel carriers, (2) polysaccharide-protein carriers, and (3) lipid carriers in the context of physicochemical and engineering factors. Physicochemical factors are encapsulation efficiency, chemical stability under gastric conditions, mechanical stability, and thermal stability of carrier matrices. Choice of carrier material also determines the encapsulation technique. Consequently, the engineering factors are related to the advantage and disadvantage of various encapsulation techniques frequently used in the literature. In addition, it was intended to address the interactions between (1) main carrier components, such as polysaccharides, proteins, and lipids themselves (in order to form chemically and mechanically stable structure); (2) main carrier components with pepsin under gastric conditions (in order to form resistant material under gastric conditions); and (3) main carrier components with EOs (in order to enhance encapsulation efficiency), as a necessary precondition for whole process optimization. Finally, different sources for obtaining natural carrier macromolecules are surveyed, especially the agro-waste materials and agricultural and food by-products. This review article highlights the bioavailability aspects of encapsulated EOs and physicochemical and engineering factors concerning natural macromolecule carriers for their target delivery and application.",
publisher = "Frontiers Media Sa, Lausanne",
journal = "Frontiers in Bioengineering and Biotechnology",
title = "Natural Macromolecules as Carriers for Essential Oils: From Extraction to Biomedical Application",
volume = "8",
doi = "10.3389/fbioe.2020.00563"
}

Dajić-Stevanović, Z., Sieniawska, E., Glowniak, K., Obradović, N.,& Pajić-Lijaković, I.. (2020). Natural Macromolecules as Carriers for Essential Oils: From Extraction to Biomedical Application. in Frontiers in Bioengineering and Biotechnology
Frontiers Media Sa, Lausanne., 8.
https://doi.org/10.3389/fbioe.2020.00563

Dajić-Stevanović Z, Sieniawska E, Glowniak K, Obradović N, Pajić-Lijaković I. Natural Macromolecules as Carriers for Essential Oils: From Extraction to Biomedical Application. in Frontiers in Bioengineering and Biotechnology. 2020;8.
doi:10.3389/fbioe.2020.00563 .

Dajić-Stevanović, Zora, Sieniawska, Elwira, Glowniak, Kazimierz, Obradović, Nataša, Pajić-Lijaković, Ivana, "Natural Macromolecules as Carriers for Essential Oils: From Extraction to Biomedical Application" in Frontiers in Bioengineering and Biotechnology, 8 (2020),
https://doi.org/10.3389/fbioe.2020.00563 . .

TY  - JOUR
AU  - Volić, Mina
AU  - Obradović, Nataša
AU  - Đorđević, Verica
AU  - Luković, Nevena
AU  - Knežević-Jugović, Zorica
AU  - Bugarski, Branko
PY  - 2020
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4367
AB  - This work aims to characterize a novel system for thyme essential oil delivery based on the combination of natural emulsifiers (soy protein and soy lecithin) and alginate, produced using the extrusion technique. The formulations are optimized concerning alginate and soy protein concentrations (both 1 to 1.5 wt.%), and consequently lecithin amount, in order to achieve spherical beads in the range 2.0 to 2.3 mm and 1.2 to 1.4 mm, wet and dry, respectively. Fourier-transform infrared analysis was performed, proving that there are interactions between all components. Lecithin-soy protein synergistic combination improved entrapment efficiency of total polyphenols (for nearly 12%) and decreased thymol release in a simulated gastric solution for nearly 35%, in comparison with beads without lecithin. The addition of lecithin enhances the thermal properties of the polysaccharide-protein systems at 50 degrees C after 3 hr of heating. The mechanical stability of the biopolymer carriers is improved with lecithin addition and the elastic modulus varied from 80.06 to 123.7 kPa, depending on the formulation. Alginate/soy protein/lecithin are effective carriers for the encapsulation, protection, and controlled release of thyme essential oil. Practical Application There is unfortunately growing human resistance to antibiotics. This work offers a novel system for effective protection and controlled release of thyme essential oil in the small intestine. The mechanical and thermal properties of the carrier were estimated as they indicate how the beads will be able to resist stress during their incorporation into food (i.e. cookies-mixing, baking). The proposed approach offers ''green advantage'' as arises from all-natural materials.
PB  - Wiley, Hoboken
T2  - Journal of Food Science
T1  - Design of biopolymer carriers enriched with natural emulsifiers for improved controlled release of thyme essential oil
EP  - 3842
IS  - 11
SP  - 3833
VL  - 85
DO  - 10.1111/1750-3841.15499
ER  - 

@article{
author = "Volić, Mina and Obradović, Nataša and Đorđević, Verica and Luković, Nevena and Knežević-Jugović, Zorica and Bugarski, Branko",
year = "2020",
abstract = "This work aims to characterize a novel system for thyme essential oil delivery based on the combination of natural emulsifiers (soy protein and soy lecithin) and alginate, produced using the extrusion technique. The formulations are optimized concerning alginate and soy protein concentrations (both 1 to 1.5 wt.%), and consequently lecithin amount, in order to achieve spherical beads in the range 2.0 to 2.3 mm and 1.2 to 1.4 mm, wet and dry, respectively. Fourier-transform infrared analysis was performed, proving that there are interactions between all components. Lecithin-soy protein synergistic combination improved entrapment efficiency of total polyphenols (for nearly 12%) and decreased thymol release in a simulated gastric solution for nearly 35%, in comparison with beads without lecithin. The addition of lecithin enhances the thermal properties of the polysaccharide-protein systems at 50 degrees C after 3 hr of heating. The mechanical stability of the biopolymer carriers is improved with lecithin addition and the elastic modulus varied from 80.06 to 123.7 kPa, depending on the formulation. Alginate/soy protein/lecithin are effective carriers for the encapsulation, protection, and controlled release of thyme essential oil. Practical Application There is unfortunately growing human resistance to antibiotics. This work offers a novel system for effective protection and controlled release of thyme essential oil in the small intestine. The mechanical and thermal properties of the carrier were estimated as they indicate how the beads will be able to resist stress during their incorporation into food (i.e. cookies-mixing, baking). The proposed approach offers ''green advantage'' as arises from all-natural materials.",
publisher = "Wiley, Hoboken",
journal = "Journal of Food Science",
title = "Design of biopolymer carriers enriched with natural emulsifiers for improved controlled release of thyme essential oil",
pages = "3842-3833",
number = "11",
volume = "85",
doi = "10.1111/1750-3841.15499"
}

Volić, M., Obradović, N., Đorđević, V., Luković, N., Knežević-Jugović, Z.,& Bugarski, B.. (2020). Design of biopolymer carriers enriched with natural emulsifiers for improved controlled release of thyme essential oil. in Journal of Food Science
Wiley, Hoboken., 85(11), 3833-3842.
https://doi.org/10.1111/1750-3841.15499

Volić M, Obradović N, Đorđević V, Luković N, Knežević-Jugović Z, Bugarski B. Design of biopolymer carriers enriched with natural emulsifiers for improved controlled release of thyme essential oil. in Journal of Food Science. 2020;85(11):3833-3842.
doi:10.1111/1750-3841.15499 .

Volić, Mina, Obradović, Nataša, Đorđević, Verica, Luković, Nevena, Knežević-Jugović, Zorica, Bugarski, Branko, "Design of biopolymer carriers enriched with natural emulsifiers for improved controlled release of thyme essential oil" in Journal of Food Science, 85, no. 11 (2020):3833-3842,
https://doi.org/10.1111/1750-3841.15499 . .

TY  - JOUR
AU  - Vujčić, Ivica
AU  - Mašić, Slobodan
AU  - Obradović, Nataša
AU  - Dramićanin, Miroslav
PY  - 2020
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4352
AB  - Wooden cultural heritage serves as a very important source of information for historians and researchers. Also, it imposes an obligation on the science to keep this legacy for future generations in a proper condition. Among other techniques of conservation of wooden cultural heritage, a very suitable method is impregnation of the wood with the polymer, whereby the wood/polymer composites are formed. This paper describes the method of preparing the wood/polymer composites based on beechwood, using five different monomer systems. The method of lyophilization and subsequent polymerization by gamma radiation was used. After lyophilization and immersing in the monomer solutions, the wood samples were dried and irradiated with a dose of 25 kGy and different gamma irradiation dose rates. The weight of the samples before and after this procedure was measured. Also, changes in the mechanical properties of wood (compression test) before and after treatment were examined. We also examined the effect of radiation dose rate on polymerization. Based on the weight differences, dose rates and materials characterization we determined which of the monomer solution and dose rate are the most suitable for making beechwood/polymer composites.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Radiation Physics and Chemistry
T1  - Preparation of beechwood/polymer composites using the method of lyophilization and gamma irradiation
VL  - 166
DO  - 10.1016/j.radphyschem.2019.108505
ER  - 

@article{
author = "Vujčić, Ivica and Mašić, Slobodan and Obradović, Nataša and Dramićanin, Miroslav",
year = "2020",
abstract = "Wooden cultural heritage serves as a very important source of information for historians and researchers. Also, it imposes an obligation on the science to keep this legacy for future generations in a proper condition. Among other techniques of conservation of wooden cultural heritage, a very suitable method is impregnation of the wood with the polymer, whereby the wood/polymer composites are formed. This paper describes the method of preparing the wood/polymer composites based on beechwood, using five different monomer systems. The method of lyophilization and subsequent polymerization by gamma radiation was used. After lyophilization and immersing in the monomer solutions, the wood samples were dried and irradiated with a dose of 25 kGy and different gamma irradiation dose rates. The weight of the samples before and after this procedure was measured. Also, changes in the mechanical properties of wood (compression test) before and after treatment were examined. We also examined the effect of radiation dose rate on polymerization. Based on the weight differences, dose rates and materials characterization we determined which of the monomer solution and dose rate are the most suitable for making beechwood/polymer composites.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Radiation Physics and Chemistry",
title = "Preparation of beechwood/polymer composites using the method of lyophilization and gamma irradiation",
volume = "166",
doi = "10.1016/j.radphyschem.2019.108505"
}

Vujčić, I., Mašić, S., Obradović, N.,& Dramićanin, M.. (2020). Preparation of beechwood/polymer composites using the method of lyophilization and gamma irradiation. in Radiation Physics and Chemistry
Pergamon-Elsevier Science Ltd, Oxford., 166.
https://doi.org/10.1016/j.radphyschem.2019.108505

Vujčić I, Mašić S, Obradović N, Dramićanin M. Preparation of beechwood/polymer composites using the method of lyophilization and gamma irradiation. in Radiation Physics and Chemistry. 2020;166.
doi:10.1016/j.radphyschem.2019.108505 .

Vujčić, Ivica, Mašić, Slobodan, Obradović, Nataša, Dramićanin, Miroslav, "Preparation of beechwood/polymer composites using the method of lyophilization and gamma irradiation" in Radiation Physics and Chemistry, 166 (2020),
https://doi.org/10.1016/j.radphyschem.2019.108505 . .

TY  - JOUR
AU  - Krunić, Tanja
AU  - Obradović, Nataša
AU  - Rakin, Marica
PY  - 2019
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4220
AB  - The main object of study is application of whey protein concentrate (WPC) and whey protein hydrolysate (WPH) for probiotic encapsulation. The controlled enzymatic hydrolysis was applied to change technological properties of WPC what leads to obtained different carriers. Probiotic carriers (beads) were made by electrostatic extrusion. Bead properties (mechanical properties, FTIR fingerprint, cell release) and parameters of fermentation were examined. According to cell release, it can be concluded that WPH build less porous matrix with alginate than WPC. Beads with WPH contained more living cells and suffered more changes during fermentation than beads with WPC. Probiotic viability in simulated gastrointestinal conditions (SGIC) is the most critical parameter for probiotic encapsulation. Probiotic encapsulated in protein-alginate beads survived SGIC with more than 96% viable cells, compared to 37.43% for free culture. According to all examined parameters, it can be concluded that WPH builds more suitable carrier for probiotic culture than WPC.
PB  - Elsevier Sci Ltd, Oxford
T2  - Food Chemistry
T1  - Application of whey protein and whey protein hydrolysate as protein based carrier for probiotic starter culture
EP  - 82
SP  - 74
VL  - 293
DO  - 10.1016/j.foodchem.2019.04.062
ER  - 

@article{
author = "Krunić, Tanja and Obradović, Nataša and Rakin, Marica",
year = "2019",
abstract = "The main object of study is application of whey protein concentrate (WPC) and whey protein hydrolysate (WPH) for probiotic encapsulation. The controlled enzymatic hydrolysis was applied to change technological properties of WPC what leads to obtained different carriers. Probiotic carriers (beads) were made by electrostatic extrusion. Bead properties (mechanical properties, FTIR fingerprint, cell release) and parameters of fermentation were examined. According to cell release, it can be concluded that WPH build less porous matrix with alginate than WPC. Beads with WPH contained more living cells and suffered more changes during fermentation than beads with WPC. Probiotic viability in simulated gastrointestinal conditions (SGIC) is the most critical parameter for probiotic encapsulation. Probiotic encapsulated in protein-alginate beads survived SGIC with more than 96% viable cells, compared to 37.43% for free culture. According to all examined parameters, it can be concluded that WPH builds more suitable carrier for probiotic culture than WPC.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Food Chemistry",
title = "Application of whey protein and whey protein hydrolysate as protein based carrier for probiotic starter culture",
pages = "82-74",
volume = "293",
doi = "10.1016/j.foodchem.2019.04.062"
}

Krunić, T., Obradović, N.,& Rakin, M.. (2019). Application of whey protein and whey protein hydrolysate as protein based carrier for probiotic starter culture. in Food Chemistry
Elsevier Sci Ltd, Oxford., 293, 74-82.
https://doi.org/10.1016/j.foodchem.2019.04.062

Krunić T, Obradović N, Rakin M. Application of whey protein and whey protein hydrolysate as protein based carrier for probiotic starter culture. in Food Chemistry. 2019;293:74-82.
doi:10.1016/j.foodchem.2019.04.062 .

Krunić, Tanja, Obradović, Nataša, Rakin, Marica, "Application of whey protein and whey protein hydrolysate as protein based carrier for probiotic starter culture" in Food Chemistry, 293 (2019):74-82,
https://doi.org/10.1016/j.foodchem.2019.04.062 . .

TY  - THES
AU  - Obradović, Nataša
PY  - 2019
UR  - http://eteze.bg.ac.rs/application/showtheses?thesesId=7465
UR  - https://fedorabg.bg.ac.rs/fedora/get/o:22298/bdef:Content/download
UR  - http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=16629769
UR  - http://nardus.mpn.gov.rs/handle/123456789/17184
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4687
AB  - Probiotic cultures are becoming more popular in human nutrition primarily because modern consumers tend to use food products that have a more positive impact on their health. The therapeutic effect of food with added live microorganisms (probiotics), especially milk products, is promoted by health professionals. In order to achieve the desired therapeutic effect of food product, probiotic bacteria have to enter the body in appropriate number and maintain viability during production and storage conditions. After consuming the product, it is important that an adequate number of cells survive conditions in the gastrointestinal tract. Preparation of a natural hydrogel carrier, which will enable the protection of the probiotic starter culture during production, storage and the gastrointestinal conditions was goal of this doctoral dissertation. Aim was full potential impact of functional food product on human health. Thesis analyzes two types of results: characterization of obtained carriers with probiotic starter culture using different encapsulation techniques and application of the carriers in preparation of functional food product. Encapsulation tehniques used: (i) extrusion (ii) by spray-drying and (iii) freeze drying (lyophilization). Production of fermented whey-based beverage was used for implementation of prepared carriers with probiotic cells...
AB  - Probiotske kulture su sve više zastupljene u ljudskoj ishrani pre svega zbog težnje savremenih potrošača da konzumiraju kvalitetne prehrambene proizvodе koji imaju i pozitivan uticaj na ljudsko zdravlje. Terapeutski efekat hrane sa dodatim živim mikroorganizmima (probioticima) posebno na primerima mlečnih proizvoda se sve više promoviše od strane zdravstvenih radnika. Probiotske bakterije, da bi ostvarile željeni terapeutski efekat, neophodno je da se u organizam unesu u odgovarajućem broju i da se održi njihova vijabilnost tokom proizvodnog procesa i skladištenja proizvoda. Isto tako, posle konzumiranja proizvoda, potrebno je da adekvatan broj ćelija preživi uslove u gastro-intestinalnom traktu. Stoga je cilj ove doktorske disertacije bio priprema nosača na bazi prirodnih hidrogelova, koji će omogućiti zaštitu probiotske starter kulture tokom proizvodnje, skladištenja proizvoda i gastrointestinalnih uslova. Osim karakterizacije dobijenih nosača sa kulturom tehnikama inkapsulacije: (i) ekstruzijom (ii) sprej-sušenjem i (iii) sušenjem smrzavanjem (liofilizacijom), analizirana je i primena nosača u proizvodnji fermentisanih napitaka na bazi surutke...
PB  - Univerzitet u Beogradu, Tehnološko-metalurški fakultet
T1  - Characterization and application of natural hydrogels for encapsulation of probiotic starter culture
T1  - Karakterizacija i primena prirodnih hidrogelova za inkapsulaciju probiotske starter kulture
UR  - https://hdl.handle.net/21.15107/rcub_nardus_17184
ER  - 

@phdthesis{
author = "Obradović, Nataša",
year = "2019",
abstract = "Probiotic cultures are becoming more popular in human nutrition primarily because modern consumers tend to use food products that have a more positive impact on their health. The therapeutic effect of food with added live microorganisms (probiotics), especially milk products, is promoted by health professionals. In order to achieve the desired therapeutic effect of food product, probiotic bacteria have to enter the body in appropriate number and maintain viability during production and storage conditions. After consuming the product, it is important that an adequate number of cells survive conditions in the gastrointestinal tract. Preparation of a natural hydrogel carrier, which will enable the protection of the probiotic starter culture during production, storage and the gastrointestinal conditions was goal of this doctoral dissertation. Aim was full potential impact of functional food product on human health. Thesis analyzes two types of results: characterization of obtained carriers with probiotic starter culture using different encapsulation techniques and application of the carriers in preparation of functional food product. Encapsulation tehniques used: (i) extrusion (ii) by spray-drying and (iii) freeze drying (lyophilization). Production of fermented whey-based beverage was used for implementation of prepared carriers with probiotic cells..., Probiotske kulture su sve više zastupljene u ljudskoj ishrani pre svega zbog težnje savremenih potrošača da konzumiraju kvalitetne prehrambene proizvodе koji imaju i pozitivan uticaj na ljudsko zdravlje. Terapeutski efekat hrane sa dodatim živim mikroorganizmima (probioticima) posebno na primerima mlečnih proizvoda se sve više promoviše od strane zdravstvenih radnika. Probiotske bakterije, da bi ostvarile željeni terapeutski efekat, neophodno je da se u organizam unesu u odgovarajućem broju i da se održi njihova vijabilnost tokom proizvodnog procesa i skladištenja proizvoda. Isto tako, posle konzumiranja proizvoda, potrebno je da adekvatan broj ćelija preživi uslove u gastro-intestinalnom traktu. Stoga je cilj ove doktorske disertacije bio priprema nosača na bazi prirodnih hidrogelova, koji će omogućiti zaštitu probiotske starter kulture tokom proizvodnje, skladištenja proizvoda i gastrointestinalnih uslova. Osim karakterizacije dobijenih nosača sa kulturom tehnikama inkapsulacije: (i) ekstruzijom (ii) sprej-sušenjem i (iii) sušenjem smrzavanjem (liofilizacijom), analizirana je i primena nosača u proizvodnji fermentisanih napitaka na bazi surutke...",
publisher = "Univerzitet u Beogradu, Tehnološko-metalurški fakultet",
title = "Characterization and application of natural hydrogels for encapsulation of probiotic starter culture, Karakterizacija i primena prirodnih hidrogelova za inkapsulaciju probiotske starter kulture",
url = "https://hdl.handle.net/21.15107/rcub_nardus_17184"
}

Obradović, N.. (2019). Characterization and application of natural hydrogels for encapsulation of probiotic starter culture. 
Univerzitet u Beogradu, Tehnološko-metalurški fakultet..
https://hdl.handle.net/21.15107/rcub_nardus_17184

Obradović N. Characterization and application of natural hydrogels for encapsulation of probiotic starter culture. 2019;.
https://hdl.handle.net/21.15107/rcub_nardus_17184 .

Obradović, Nataša, "Characterization and application of natural hydrogels for encapsulation of probiotic starter culture" (2019),
https://hdl.handle.net/21.15107/rcub_nardus_17184 .

TY  - JOUR
AU  - Krunić, Tanja
AU  - Obradović, Nataša
AU  - Bulatović, Maja
AU  - Vukašinović-Sekulić, Maja
AU  - Trifković, Kata T.
AU  - Rakin, Marica
PY  - 2017
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3745
AB  - The main objectives of this paper were to study the influence of the carrier material used for encapsulation and of bead size to fermentative activity and viability of the dairy starter culture Lactoferm ABY 6. Encapsulation of yoghurt culture in beads with diameter of 1 mm provides better results than encapsulation in beads with larger diameter. Alginate beads and chitosan coated beads have proved to be a strong barrier for nutrients from substrate, so samples with those beads have lower viable cell count, lower titratable acidity and higher pH value after 5h of fermentation at 42 degrees C, than samples with WPC-alginate beads. Also those beads have significantly (P  lt  0.05) lower cell leaking, than WPC-alginate beads and lower antioxidant capacity. Encapsulation of yoghurt culture in WPC-alginate carrier with diameter of approximately 1mm provided the best characteristics for fermented product. Samples with these beads have significantly (P  lt  0.05) higher increase of viable cell number after fermentation, despite of major cell leaking (19.7%). Moreover, sample with these beads have the highest titratable acidity, the lowest pH value after fermentation (the best fermentative activity) and the best antioxidant characteristics.
PB  - Savez hemijskih inženjera, Beograd
T2  - Hemijska industrija
T1  - Impact of carrier material on fermentative activity of encapsulated yoghurt culture in whey based substrate
EP  - 48
IS  - 1
SP  - 41
VL  - 71
DO  - 10.2298/HEMIND150717016K
ER  - 

@article{
author = "Krunić, Tanja and Obradović, Nataša and Bulatović, Maja and Vukašinović-Sekulić, Maja and Trifković, Kata T. and Rakin, Marica",
year = "2017",
abstract = "The main objectives of this paper were to study the influence of the carrier material used for encapsulation and of bead size to fermentative activity and viability of the dairy starter culture Lactoferm ABY 6. Encapsulation of yoghurt culture in beads with diameter of 1 mm provides better results than encapsulation in beads with larger diameter. Alginate beads and chitosan coated beads have proved to be a strong barrier for nutrients from substrate, so samples with those beads have lower viable cell count, lower titratable acidity and higher pH value after 5h of fermentation at 42 degrees C, than samples with WPC-alginate beads. Also those beads have significantly (P  lt  0.05) lower cell leaking, than WPC-alginate beads and lower antioxidant capacity. Encapsulation of yoghurt culture in WPC-alginate carrier with diameter of approximately 1mm provided the best characteristics for fermented product. Samples with these beads have significantly (P  lt  0.05) higher increase of viable cell number after fermentation, despite of major cell leaking (19.7%). Moreover, sample with these beads have the highest titratable acidity, the lowest pH value after fermentation (the best fermentative activity) and the best antioxidant characteristics.",
publisher = "Savez hemijskih inženjera, Beograd",
journal = "Hemijska industrija",
title = "Impact of carrier material on fermentative activity of encapsulated yoghurt culture in whey based substrate",
pages = "48-41",
number = "1",
volume = "71",
doi = "10.2298/HEMIND150717016K"
}

Krunić, T., Obradović, N., Bulatović, M., Vukašinović-Sekulić, M., Trifković, K. T.,& Rakin, M.. (2017). Impact of carrier material on fermentative activity of encapsulated yoghurt culture in whey based substrate. in Hemijska industrija
Savez hemijskih inženjera, Beograd., 71(1), 41-48.
https://doi.org/10.2298/HEMIND150717016K

Krunić T, Obradović N, Bulatović M, Vukašinović-Sekulić M, Trifković KT, Rakin M. Impact of carrier material on fermentative activity of encapsulated yoghurt culture in whey based substrate. in Hemijska industrija. 2017;71(1):41-48.
doi:10.2298/HEMIND150717016K .

Krunić, Tanja, Obradović, Nataša, Bulatović, Maja, Vukašinović-Sekulić, Maja, Trifković, Kata T., Rakin, Marica, "Impact of carrier material on fermentative activity of encapsulated yoghurt culture in whey based substrate" in Hemijska industrija, 71, no. 1 (2017):41-48,
https://doi.org/10.2298/HEMIND150717016K . .

TY  - JOUR
AU  - Krunić, Tanja
AU  - Bulatović, Maja
AU  - Obradović, Nataša
AU  - Vukašinović-Sekulić, Maja
AU  - Rakin, Marica
PY  - 2016
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3478
AB  - BACKGROUND: The main objectives of the paper were to study influence of immobilisation of dairy starter culture 'Lactoferm ABY 6' on fermentation and probiotic potential of fermented whey-based substrate. RESULTS: Fermentation with free cells takes 1.5 h less than fermentation with encapsulated cells, but samples with encapsulated cells have better characteristics after 28 days of storage. Chitosan coating provides additional protection of cells in bile salt solution (95.86% of viable cells compared to the initial number) and simulated gastric juice (37.8% for pH 2.5) compared to the alginate beads (94.54% in bile salt solution and 36.18% in simulated gastric juice for pH 2.5). Free cells had a drastic reduction in the number of viable cells (83.0% in bile salt solution and no viable cells in simulated gastric juice for pH 2.5). CONCLUSION: Samples with alginate beads and chitosan-coated alginate beads have significantly (P  lt  0.05) higher viable cell count than samples with free cells, during 4 h monitoring survival at pH 2.5, pH 3.0 and 0.3% bovine bile solution. These beads can be used to improve survival of probiotic cells in fermented whey-based beverage during storage and consummation, which improves the quality of the product.
PB  - Wiley-Blackwell, Hoboken
T2  - Journal of the Science of Food and Agriculture
T1  - Effect of immobilisation materials on viability and fermentation activity of dairy starter culture in whey-based substrate
EP  - 1729
IS  - 5
SP  - 1723
VL  - 96
DO  - 10.1002/jsfa.7278
ER  - 

@article{
author = "Krunić, Tanja and Bulatović, Maja and Obradović, Nataša and Vukašinović-Sekulić, Maja and Rakin, Marica",
year = "2016",
abstract = "BACKGROUND: The main objectives of the paper were to study influence of immobilisation of dairy starter culture 'Lactoferm ABY 6' on fermentation and probiotic potential of fermented whey-based substrate. RESULTS: Fermentation with free cells takes 1.5 h less than fermentation with encapsulated cells, but samples with encapsulated cells have better characteristics after 28 days of storage. Chitosan coating provides additional protection of cells in bile salt solution (95.86% of viable cells compared to the initial number) and simulated gastric juice (37.8% for pH 2.5) compared to the alginate beads (94.54% in bile salt solution and 36.18% in simulated gastric juice for pH 2.5). Free cells had a drastic reduction in the number of viable cells (83.0% in bile salt solution and no viable cells in simulated gastric juice for pH 2.5). CONCLUSION: Samples with alginate beads and chitosan-coated alginate beads have significantly (P  lt  0.05) higher viable cell count than samples with free cells, during 4 h monitoring survival at pH 2.5, pH 3.0 and 0.3% bovine bile solution. These beads can be used to improve survival of probiotic cells in fermented whey-based beverage during storage and consummation, which improves the quality of the product.",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Journal of the Science of Food and Agriculture",
title = "Effect of immobilisation materials on viability and fermentation activity of dairy starter culture in whey-based substrate",
pages = "1729-1723",
number = "5",
volume = "96",
doi = "10.1002/jsfa.7278"
}

Krunić, T., Bulatović, M., Obradović, N., Vukašinović-Sekulić, M.,& Rakin, M.. (2016). Effect of immobilisation materials on viability and fermentation activity of dairy starter culture in whey-based substrate. in Journal of the Science of Food and Agriculture
Wiley-Blackwell, Hoboken., 96(5), 1723-1729.
https://doi.org/10.1002/jsfa.7278

Krunić T, Bulatović M, Obradović N, Vukašinović-Sekulić M, Rakin M. Effect of immobilisation materials on viability and fermentation activity of dairy starter culture in whey-based substrate. in Journal of the Science of Food and Agriculture. 2016;96(5):1723-1729.
doi:10.1002/jsfa.7278 .

Krunić, Tanja, Bulatović, Maja, Obradović, Nataša, Vukašinović-Sekulić, Maja, Rakin, Marica, "Effect of immobilisation materials on viability and fermentation activity of dairy starter culture in whey-based substrate" in Journal of the Science of Food and Agriculture, 96, no. 5 (2016):1723-1729,
https://doi.org/10.1002/jsfa.7278 . .

TY  - JOUR
AU  - Obradović, Nataša
AU  - Krunić, Tanja
AU  - Damnjanović, Ivana D.
AU  - Vukašinović-Sekulić, Maja
AU  - Rakin, Marica
AU  - Rakin, Marko
AU  - Bugarski, Branko
PY  - 2015
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2907
AB  - Biopolymer carriers are widely used for immobilization of the bioactive compounds and different type of the cells. The aim of this study was to analyze influence of whey proteins addition on mechanical strength of Ca-alginate beads and survival of probiotic bacteria during the whey fermentation process. Mechanical stability of beads was analyzed up to 30% of bead's deformation using compression method between two flat plates. The fermentation was carried out with probiotic starter culture ABY-6 until pH=4.6. The results indicate that the addition of whey proteins in alginate solution increases strength of the beads after fermentation in comparison with alginate beads without proteins and extends the shelf life of products through improving the viability of probiotics.
AB  - Biopolimerni nosači su najčešće korišćenih nosači za imobilizaciju bioaktivnih jedinjenja i različite vrste ćelija. Cilj ovog istraživanja je bila analiza uticaja dodatka proteina surutke na mehaničku čvrstoću kalcijum alginatnih čestica i preživljavanje probiotskih bakterija tokom procesa fermentacije surutke. Mehanička stabilnost nosača je analizirana do 30% deformacije čestica metodom kompresije između dve ravne ploče. Fermentacija se odvijala pomoću probiotiske starter kulture ABY-6 do vrednosti pH = 4.6. Rezultati ukazuju da dodatak proteina surutke u alginatni rastvor povećava čvsrtoću čestica nakon fermentacije u poređenju sa alginatnim česticama bez proteina i produžava rok trajanja proizvoda kroz unapređenje preživljavanja probiotskih bakterija .
PB  - Savez inženjera i tehničara Srbije, Beograd
T2  - Tehnika
T1  - Influence of whey proteins addition on mechanical stability of biopolymer beads with immobilized probiotics
T1  - Uticaj dodatka proteina surutke na mehaničku stabilnost biopolimernih nosača sa imobilisanim probioticima
EP  - 400
IS  - 3
SP  - 397
VL  - 70
DO  - 10.5937/tehnika1503397O
ER  - 

@article{
author = "Obradović, Nataša and Krunić, Tanja and Damnjanović, Ivana D. and Vukašinović-Sekulić, Maja and Rakin, Marica and Rakin, Marko and Bugarski, Branko",
year = "2015",
abstract = "Biopolymer carriers are widely used for immobilization of the bioactive compounds and different type of the cells. The aim of this study was to analyze influence of whey proteins addition on mechanical strength of Ca-alginate beads and survival of probiotic bacteria during the whey fermentation process. Mechanical stability of beads was analyzed up to 30% of bead's deformation using compression method between two flat plates. The fermentation was carried out with probiotic starter culture ABY-6 until pH=4.6. The results indicate that the addition of whey proteins in alginate solution increases strength of the beads after fermentation in comparison with alginate beads without proteins and extends the shelf life of products through improving the viability of probiotics., Biopolimerni nosači su najčešće korišćenih nosači za imobilizaciju bioaktivnih jedinjenja i različite vrste ćelija. Cilj ovog istraživanja je bila analiza uticaja dodatka proteina surutke na mehaničku čvrstoću kalcijum alginatnih čestica i preživljavanje probiotskih bakterija tokom procesa fermentacije surutke. Mehanička stabilnost nosača je analizirana do 30% deformacije čestica metodom kompresije između dve ravne ploče. Fermentacija se odvijala pomoću probiotiske starter kulture ABY-6 do vrednosti pH = 4.6. Rezultati ukazuju da dodatak proteina surutke u alginatni rastvor povećava čvsrtoću čestica nakon fermentacije u poređenju sa alginatnim česticama bez proteina i produžava rok trajanja proizvoda kroz unapređenje preživljavanja probiotskih bakterija .",
publisher = "Savez inženjera i tehničara Srbije, Beograd",
journal = "Tehnika",
title = "Influence of whey proteins addition on mechanical stability of biopolymer beads with immobilized probiotics, Uticaj dodatka proteina surutke na mehaničku stabilnost biopolimernih nosača sa imobilisanim probioticima",
pages = "400-397",
number = "3",
volume = "70",
doi = "10.5937/tehnika1503397O"
}

Obradović, N., Krunić, T., Damnjanović, I. D., Vukašinović-Sekulić, M., Rakin, M., Rakin, M.,& Bugarski, B.. (2015). Influence of whey proteins addition on mechanical stability of biopolymer beads with immobilized probiotics. in Tehnika
Savez inženjera i tehničara Srbije, Beograd., 70(3), 397-400.
https://doi.org/10.5937/tehnika1503397O

Obradović N, Krunić T, Damnjanović ID, Vukašinović-Sekulić M, Rakin M, Rakin M, Bugarski B. Influence of whey proteins addition on mechanical stability of biopolymer beads with immobilized probiotics. in Tehnika. 2015;70(3):397-400.
doi:10.5937/tehnika1503397O .

Obradović, Nataša, Krunić, Tanja, Damnjanović, Ivana D., Vukašinović-Sekulić, Maja, Rakin, Marica, Rakin, Marko, Bugarski, Branko, "Influence of whey proteins addition on mechanical stability of biopolymer beads with immobilized probiotics" in Tehnika, 70, no. 3 (2015):397-400,
https://doi.org/10.5937/tehnika1503397O . .

TY  - JOUR
AU  - Obradović, Nataša
AU  - Krunić, Tanja
AU  - Trifković, Kata T.
AU  - Bulatović, Maja
AU  - Rakin, Marko
AU  - Rakin, Marica
AU  - Bugarski, Branko
PY  - 2015
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3067
AB  - The aim of this study was to improve the mechanical stability of biopolymer carriers and cell viability with addition of chitosan coating during fermentation process and product storage. Dairy starter culture (1% (w/v)) was diluted in whey and mixed with sodium alginate solution and the beads were made using extrusion technique. The mechanical stability of coated and uncoated beads, the release behavior, and the viability of encapsulated probiotic dairy starter culture in fermented whey beverages were analyzed. The mechanical properties of the beads were determined according to force-displacement and engineering stress-strain curves obtained after compression testing. It was observed that addition of chitosan as a coating on the beads as well as the fermentation process increased the elastic modulus of the calcium alginate-whey beads and cell survival. The current study revealed that the coating did not significantly improve the viability of probiotics during the fermentation but had an important influence on preservation of the strength of the carrier during storage. Our results indicate that whey-based substrate has positive effect on the mechanical stability of biopolymer beads with encapsulated probiotics.
PB  - Hindawi Ltd, London
T2  - International Journal of Polymer Science
T1  - Influence of Chitosan Coating on Mechanical Stability of Biopolymer Carriers with Probiotic Starter Culture in Fermented Whey Beverages
VL  - 2015
DO  - 10.1155/2015/732858
ER  - 

@article{
author = "Obradović, Nataša and Krunić, Tanja and Trifković, Kata T. and Bulatović, Maja and Rakin, Marko and Rakin, Marica and Bugarski, Branko",
year = "2015",
abstract = "The aim of this study was to improve the mechanical stability of biopolymer carriers and cell viability with addition of chitosan coating during fermentation process and product storage. Dairy starter culture (1% (w/v)) was diluted in whey and mixed with sodium alginate solution and the beads were made using extrusion technique. The mechanical stability of coated and uncoated beads, the release behavior, and the viability of encapsulated probiotic dairy starter culture in fermented whey beverages were analyzed. The mechanical properties of the beads were determined according to force-displacement and engineering stress-strain curves obtained after compression testing. It was observed that addition of chitosan as a coating on the beads as well as the fermentation process increased the elastic modulus of the calcium alginate-whey beads and cell survival. The current study revealed that the coating did not significantly improve the viability of probiotics during the fermentation but had an important influence on preservation of the strength of the carrier during storage. Our results indicate that whey-based substrate has positive effect on the mechanical stability of biopolymer beads with encapsulated probiotics.",
publisher = "Hindawi Ltd, London",
journal = "International Journal of Polymer Science",
title = "Influence of Chitosan Coating on Mechanical Stability of Biopolymer Carriers with Probiotic Starter Culture in Fermented Whey Beverages",
volume = "2015",
doi = "10.1155/2015/732858"
}

Obradović, N., Krunić, T., Trifković, K. T., Bulatović, M., Rakin, M., Rakin, M.,& Bugarski, B.. (2015). Influence of Chitosan Coating on Mechanical Stability of Biopolymer Carriers with Probiotic Starter Culture in Fermented Whey Beverages. in International Journal of Polymer Science
Hindawi Ltd, London., 2015.
https://doi.org/10.1155/2015/732858

Obradović N, Krunić T, Trifković KT, Bulatović M, Rakin M, Rakin M, Bugarski B. Influence of Chitosan Coating on Mechanical Stability of Biopolymer Carriers with Probiotic Starter Culture in Fermented Whey Beverages. in International Journal of Polymer Science. 2015;2015.
doi:10.1155/2015/732858 .

Obradović, Nataša, Krunić, Tanja, Trifković, Kata T., Bulatović, Maja, Rakin, Marko, Rakin, Marica, Bugarski, Branko, "Influence of Chitosan Coating on Mechanical Stability of Biopolymer Carriers with Probiotic Starter Culture in Fermented Whey Beverages" in International Journal of Polymer Science, 2015 (2015),
https://doi.org/10.1155/2015/732858 . .

TY  - JOUR
AU  - Dimić, Ivana
AU  - Cvijović-Alagić, Ivana
AU  - Obradović, Nataša
AU  - Petrović, Jelena
AU  - Putić, Slaviša
AU  - Rakin, Marko
AU  - Bugarski, Branko
PY  - 2015
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3087
AB  - Metallic materials, such as Co-Cr-Mo alloys, are exposed to aggressive conditions in the oral cavity that represents an ideal environment for metallic ion release and biodegradation. The metallic ions released from dental materials can cause local and/or systemic adverse effects in the human body. Therefore, dental materials are required to possess appropriate mechanical, physical, chemical and biological properties. The biocompatibility of metallic materials is very important for dental applications. Accordingly, the aim of this study was to examine metallic ion release and cytotoxicity of Co-30Cr-5Mo cast alloy as the initial phase of biocompatibility evaluation. Determination of the viability of human (MRC-5) and animal (L929) fibroblast cells were conducted using three in vitro test methods: the colorimetric methyl-thiazol-tetrazolium (MTT) test, the dye exclusion test (DET) and the agar diffusion test (ADT). Furthermore, the morphology and growth of the cells were analyzed using scanning electron microscopy (SEM). The obtained results indicated that Co-30Cr-5Mo alloy did not release harmful elements in concentrations high enough to have detrimental effects on human and animal fibroblasts under the given experimental conditions. Moreover, the fibroblast cells showed good adhesion on the surface of the Co-30Cr-5Mo alloy. Therefore, it could be concluded that Co-30Cr-5Mo alloy is a biocompatible material that could be safely used in dentistry.
PB  - Srpsko hemijsko društvo, Beograd
T2  - Journal of the Serbian Chemical Society
T1  - In vitro biocompatibility assessment of Co-Cr-Mo dental cast alloy
EP  - 1552
IS  - 12
SP  - 1541
VL  - 80
DO  - 10.2298/JSC150505070M
ER  - 

@article{
author = "Dimić, Ivana and Cvijović-Alagić, Ivana and Obradović, Nataša and Petrović, Jelena and Putić, Slaviša and Rakin, Marko and Bugarski, Branko",
year = "2015",
abstract = "Metallic materials, such as Co-Cr-Mo alloys, are exposed to aggressive conditions in the oral cavity that represents an ideal environment for metallic ion release and biodegradation. The metallic ions released from dental materials can cause local and/or systemic adverse effects in the human body. Therefore, dental materials are required to possess appropriate mechanical, physical, chemical and biological properties. The biocompatibility of metallic materials is very important for dental applications. Accordingly, the aim of this study was to examine metallic ion release and cytotoxicity of Co-30Cr-5Mo cast alloy as the initial phase of biocompatibility evaluation. Determination of the viability of human (MRC-5) and animal (L929) fibroblast cells were conducted using three in vitro test methods: the colorimetric methyl-thiazol-tetrazolium (MTT) test, the dye exclusion test (DET) and the agar diffusion test (ADT). Furthermore, the morphology and growth of the cells were analyzed using scanning electron microscopy (SEM). The obtained results indicated that Co-30Cr-5Mo alloy did not release harmful elements in concentrations high enough to have detrimental effects on human and animal fibroblasts under the given experimental conditions. Moreover, the fibroblast cells showed good adhesion on the surface of the Co-30Cr-5Mo alloy. Therefore, it could be concluded that Co-30Cr-5Mo alloy is a biocompatible material that could be safely used in dentistry.",
publisher = "Srpsko hemijsko društvo, Beograd",
journal = "Journal of the Serbian Chemical Society",
title = "In vitro biocompatibility assessment of Co-Cr-Mo dental cast alloy",
pages = "1552-1541",
number = "12",
volume = "80",
doi = "10.2298/JSC150505070M"
}

Dimić, I., Cvijović-Alagić, I., Obradović, N., Petrović, J., Putić, S., Rakin, M.,& Bugarski, B.. (2015). In vitro biocompatibility assessment of Co-Cr-Mo dental cast alloy. in Journal of the Serbian Chemical Society
Srpsko hemijsko društvo, Beograd., 80(12), 1541-1552.
https://doi.org/10.2298/JSC150505070M

Dimić I, Cvijović-Alagić I, Obradović N, Petrović J, Putić S, Rakin M, Bugarski B. In vitro biocompatibility assessment of Co-Cr-Mo dental cast alloy. in Journal of the Serbian Chemical Society. 2015;80(12):1541-1552.
doi:10.2298/JSC150505070M .

Dimić, Ivana, Cvijović-Alagić, Ivana, Obradović, Nataša, Petrović, Jelena, Putić, Slaviša, Rakin, Marko, Bugarski, Branko, "In vitro biocompatibility assessment of Co-Cr-Mo dental cast alloy" in Journal of the Serbian Chemical Society, 80, no. 12 (2015):1541-1552,
https://doi.org/10.2298/JSC150505070M . .

TY  - JOUR
AU  - Tomović, Nataša
AU  - Trifković, Kata T.
AU  - Rakin, Marko
AU  - Rakin, Marica
AU  - Bugarski, Branko
PY  - 2015
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2925
AB  - Hydrogel particles are often used as carriers for the immobilization of enzymes, polyphenolic antioxidants, whole microbial, plant or mammalian cells. In many processes, the mechanical properties of alginate particles are essential due to their exposure to mechanical forces in production process. Determination and improvement of hydrogels mechanical properties is very important in prevention of the undesirable side effects during the manufacturing process and product application. The aim of this study was to define the mechanical properties of single particles submerged in water and in dry conditions using the compression method between two flat surfaces. The results indicated that the formulation of alginate beads and water loss during compression have significant influence on their mechanical behavior and stiffness. Calcium-alginate particles were produced using an electrostatic droplet extrusion technique, with an initial sodium alginate concentration of 1.5%, w/V, and calcium chloride (2.0%, w/V) as the gelling solution. The research findings were used to determine the influence of working conditions, sample deformation (10-50%) and different compression speeds (1-50 mm/min) on the mechanical strength of alginate beads. The Young's moduli and maximal forces for investigated deformation percentage of the alginate particles were determined from generated force-displacement and stress-strain curves during compression.
AB  - Hidrogel čestice su najčešće korišćeni nosači prilikom imobilizacije enzima, polifenolnih jedinjenja, biljnih i životinjskih ćelija. U mnogim procesima čestice hidrogela su izložene mehaničkim silama koje mogu izazvati deformaciju, čime se ograničava njihova praktična primena. Definisanje i unapređenje mehaničkih svojstava hidrogelova je veoma važno u cilju sprečavanja neželjenih efekata u toku samog proizvodnog procesa, kao i dalje primene proizvoda. Cilj ovog rada je bilo ispitivanje mehaničkih svojstava pojedinačnih čestica potopljenih u vodi, kao i na vazduhu (suvi uslovi) pomoću metode kompresije između dve ravne površine. Rezultati su ukazali da sastav alginatnih čestica i gubitak vode tokom procesa kompresije imaju značajan uticaj na njihove mehaničke karakteristike i čvrstoću. Kalcijum-alginatne čestice su dobijene postupkom elektrostatičke ekstruzije, potiskivanjem natrijum-alginata (1.5%) kroz špric pomoću infuzione pumpe u rastvor kalcijum-hlorida (2.0%). Dobijeni rezultati su iskorišćeni za ispitivanja uticaja radnih uslova, procenta deformacije (10-50%) i različitih vrednosti brzine sabijanja (1-50mm/min) uzorka na mehanička svojstva nosača. Modul elastičnosti kao i maksimalna sila za ispitivani procenat deformacije alginatnih čestica su određeni pomoću dobijenih eksperimentalnih krivih zavisnosti sile od pomeranja i napona od deformacije tokom procesa kompresije.
PB  - Association of the Chemical Engineers of Serbia
T2  - Chemical Industry & Chemical Engineering Quarterly
T1  - Influence of compression speed and deformation percentage on mechanical properties of calcium alginate particles
T1  - Uticaj brzine kompresije i procenta deformacije na mehaničke karakteristike kalcijum alginatnih čestica
EP  - 417
IS  - 3
SP  - 411
VL  - 21
DO  - 10.2298/CICEQ140228043T
ER  - 

@article{
author = "Tomović, Nataša and Trifković, Kata T. and Rakin, Marko and Rakin, Marica and Bugarski, Branko",
year = "2015",
abstract = "Hydrogel particles are often used as carriers for the immobilization of enzymes, polyphenolic antioxidants, whole microbial, plant or mammalian cells. In many processes, the mechanical properties of alginate particles are essential due to their exposure to mechanical forces in production process. Determination and improvement of hydrogels mechanical properties is very important in prevention of the undesirable side effects during the manufacturing process and product application. The aim of this study was to define the mechanical properties of single particles submerged in water and in dry conditions using the compression method between two flat surfaces. The results indicated that the formulation of alginate beads and water loss during compression have significant influence on their mechanical behavior and stiffness. Calcium-alginate particles were produced using an electrostatic droplet extrusion technique, with an initial sodium alginate concentration of 1.5%, w/V, and calcium chloride (2.0%, w/V) as the gelling solution. The research findings were used to determine the influence of working conditions, sample deformation (10-50%) and different compression speeds (1-50 mm/min) on the mechanical strength of alginate beads. The Young's moduli and maximal forces for investigated deformation percentage of the alginate particles were determined from generated force-displacement and stress-strain curves during compression., Hidrogel čestice su najčešće korišćeni nosači prilikom imobilizacije enzima, polifenolnih jedinjenja, biljnih i životinjskih ćelija. U mnogim procesima čestice hidrogela su izložene mehaničkim silama koje mogu izazvati deformaciju, čime se ograničava njihova praktična primena. Definisanje i unapređenje mehaničkih svojstava hidrogelova je veoma važno u cilju sprečavanja neželjenih efekata u toku samog proizvodnog procesa, kao i dalje primene proizvoda. Cilj ovog rada je bilo ispitivanje mehaničkih svojstava pojedinačnih čestica potopljenih u vodi, kao i na vazduhu (suvi uslovi) pomoću metode kompresije između dve ravne površine. Rezultati su ukazali da sastav alginatnih čestica i gubitak vode tokom procesa kompresije imaju značajan uticaj na njihove mehaničke karakteristike i čvrstoću. Kalcijum-alginatne čestice su dobijene postupkom elektrostatičke ekstruzije, potiskivanjem natrijum-alginata (1.5%) kroz špric pomoću infuzione pumpe u rastvor kalcijum-hlorida (2.0%). Dobijeni rezultati su iskorišćeni za ispitivanja uticaja radnih uslova, procenta deformacije (10-50%) i različitih vrednosti brzine sabijanja (1-50mm/min) uzorka na mehanička svojstva nosača. Modul elastičnosti kao i maksimalna sila za ispitivani procenat deformacije alginatnih čestica su određeni pomoću dobijenih eksperimentalnih krivih zavisnosti sile od pomeranja i napona od deformacije tokom procesa kompresije.",
publisher = "Association of the Chemical Engineers of Serbia",
journal = "Chemical Industry & Chemical Engineering Quarterly",
title = "Influence of compression speed and deformation percentage on mechanical properties of calcium alginate particles, Uticaj brzine kompresije i procenta deformacije na mehaničke karakteristike kalcijum alginatnih čestica",
pages = "417-411",
number = "3",
volume = "21",
doi = "10.2298/CICEQ140228043T"
}

Tomović, N., Trifković, K. T., Rakin, M., Rakin, M.,& Bugarski, B.. (2015). Influence of compression speed and deformation percentage on mechanical properties of calcium alginate particles. in Chemical Industry & Chemical Engineering Quarterly
Association of the Chemical Engineers of Serbia., 21(3), 411-417.
https://doi.org/10.2298/CICEQ140228043T

Tomović N, Trifković KT, Rakin M, Rakin M, Bugarski B. Influence of compression speed and deformation percentage on mechanical properties of calcium alginate particles. in Chemical Industry & Chemical Engineering Quarterly. 2015;21(3):411-417.
doi:10.2298/CICEQ140228043T .

Tomović, Nataša, Trifković, Kata T., Rakin, Marko, Rakin, Marica, Bugarski, Branko, "Influence of compression speed and deformation percentage on mechanical properties of calcium alginate particles" in Chemical Industry & Chemical Engineering Quarterly, 21, no. 3 (2015):411-417,
https://doi.org/10.2298/CICEQ140228043T . .

TY  - CONF
AU  - Krunić, Tanja
AU  - Obradović, Nataša
AU  - Bulatović, Maja
AU  - Vukašinović Sekulić, Maja
AU  - Mojović, Ljiljana
AU  - Rakin, Marica
PY  - 2014
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7376
AB  - The aim of this paper was to study fermentative activity by free and immobilized probiotic ABY-6
culture in whey substrate enriched with milk. Viability of free and immobilized probiotic cells in
simulated gastrointestinal conditions was evaluated. In order to improve probiotic character of
immobilized cells, the influence of beads wall material composition (alginate, alginate beads with cells
coated by whey protein and chitosan–coated alginate beads) was examined. The beads were
prepared using electrostatic extrusion technique. Alginate cell suspension was added dropwise by a
syringe pump into a gelling solution. After 5.5h of fermentation, enumeration of viable cells was
performed by determining the number of colony-forming units on MRS agar plates after incubation at
37 ˚C for 48 h using pour plate technique on MRS agar.
The results showed that immobilized cells beads have much better potential for survival in
gastrointestinal conditions than free cells. Immobilization of probiotic cells in calcium alginate beads
coated with chitosan resulted in better survival than both type of non-coated beads in simulated
gastrointestinal conditions. These beads can be used to improve preservation of probiotic cells in
fermented whey based beverage.
PB  - Novi Sad : University, Institute of Food Technology
C3  - Proceedings / II International Congress Food Technology, Quality and Safety, Food Tech Congress, 28-30. 10. 2014. Novi Sad, Serbia
T1  - Fermentative activity and viability of immobilized probiotic starter culture ABY-6 in whey based substrates
EP  - 302
SP  - 297
UR  - https://hdl.handle.net/21.15107/rcub_technorep_7376
ER  - 

@conference{
author = "Krunić, Tanja and Obradović, Nataša and Bulatović, Maja and Vukašinović Sekulić, Maja and Mojović, Ljiljana and Rakin, Marica",
year = "2014",
abstract = "The aim of this paper was to study fermentative activity by free and immobilized probiotic ABY-6
culture in whey substrate enriched with milk. Viability of free and immobilized probiotic cells in
simulated gastrointestinal conditions was evaluated. In order to improve probiotic character of
immobilized cells, the influence of beads wall material composition (alginate, alginate beads with cells
coated by whey protein and chitosan–coated alginate beads) was examined. The beads were
prepared using electrostatic extrusion technique. Alginate cell suspension was added dropwise by a
syringe pump into a gelling solution. After 5.5h of fermentation, enumeration of viable cells was
performed by determining the number of colony-forming units on MRS agar plates after incubation at
37 ˚C for 48 h using pour plate technique on MRS agar.
The results showed that immobilized cells beads have much better potential for survival in
gastrointestinal conditions than free cells. Immobilization of probiotic cells in calcium alginate beads
coated with chitosan resulted in better survival than both type of non-coated beads in simulated
gastrointestinal conditions. These beads can be used to improve preservation of probiotic cells in
fermented whey based beverage.",
publisher = "Novi Sad : University, Institute of Food Technology",
journal = "Proceedings / II International Congress Food Technology, Quality and Safety, Food Tech Congress, 28-30. 10. 2014. Novi Sad, Serbia",
title = "Fermentative activity and viability of immobilized probiotic starter culture ABY-6 in whey based substrates",
pages = "302-297",
url = "https://hdl.handle.net/21.15107/rcub_technorep_7376"
}

Krunić, T., Obradović, N., Bulatović, M., Vukašinović Sekulić, M., Mojović, L.,& Rakin, M.. (2014). Fermentative activity and viability of immobilized probiotic starter culture ABY-6 in whey based substrates. in Proceedings / II International Congress Food Technology, Quality and Safety, Food Tech Congress, 28-30. 10. 2014. Novi Sad, Serbia
Novi Sad : University, Institute of Food Technology., 297-302.
https://hdl.handle.net/21.15107/rcub_technorep_7376

Krunić T, Obradović N, Bulatović M, Vukašinović Sekulić M, Mojović L, Rakin M. Fermentative activity and viability of immobilized probiotic starter culture ABY-6 in whey based substrates. in Proceedings / II International Congress Food Technology, Quality and Safety, Food Tech Congress, 28-30. 10. 2014. Novi Sad, Serbia. 2014;:297-302.
https://hdl.handle.net/21.15107/rcub_technorep_7376 .

Krunić, Tanja, Obradović, Nataša, Bulatović, Maja, Vukašinović Sekulić, Maja, Mojović, Ljiljana, Rakin, Marica, "Fermentative activity and viability of immobilized probiotic starter culture ABY-6 in whey based substrates" in Proceedings / II International Congress Food Technology, Quality and Safety, Food Tech Congress, 28-30. 10. 2014. Novi Sad, Serbia (2014):297-302,
https://hdl.handle.net/21.15107/rcub_technorep_7376 .