TY  - CONF
AU  - Lukić, Ivana
AU  - Stamenić, Marko
AU  - Tadić, Vanja
AU  - Skala, Dejan
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6984
AB  - The high potential of industrial hemp (Cannabis sativa), mainly due to the high-quality fibers and bioactive compounds, coupled with the decriminalization and/or legalization in some regions of the world, led to the steady growth of scientific research over the past couple of decades [1, 2]. From an industrial point of view, cannabinoids, primarily non-psychotropic cannabidiol (CBD), are considered the most valuable compounds, as they possess a broad range of therapeutic properties, such as anxiolytic, neuroprotective, antibiotic, antiinflammatory activity, and anti-oxidant. The hemp industry has already adopted supercritical carbon dioxide scCO2 extraction as a well-established process since it is a promising ‘green’ alternative to conventional extraction methods, selective, with short processing time, and low impact on the environment.
In the present study, the preliminary analysis was performed to determine the influence of different
scCO2 densities based on the different combinations of process pressure and temperature on the kinetics of supercritical extraction of Cannabis sativa, extract yield and the chemical composition of obtained extracts. Experiments were performed at temperatures ranging from 40 to 60 °C and pressures ranging from 10 to 30 MPa. Prior to extraction, the plant material was grounded and submitted to decarboxylation process at 120 °C for 1 h, in order to transform cannabidiolic acid, which is the precursor present in the plant material, to CBD.
Experimental design for optimization plays an important role both in science and industry, because it allows evaluation of the effects of multiple factors and their interactions on one or more response variables. The present work was focused on the further optimization of scCO2 extraction with the aim to maximize the yield of extracts with high recoveries of CBD, by means of response surface methodology (RSM), based on a central composite design (CCD) using Design of Experiment software (Stat-Ease, Design Expert). For this purpose, independent variables were temperature (40-60 °C), pressure (10-30 MPa), and scCO2 consumption (20-60 gCO2/gplant).
The results showed that supercritical CO2 extraction enabled the achievement of up to 5% of total extract yield and 266–435 mg/g of CBD depending on the conditions. The high pressure and temperature, 30 MPa 60 °C, respectively, conditions that correspond to the highest density of the scCO2, generated the highest extract yield. On the contrary, lower pressure, corresponding to the lower density of CO2 was favourable for achieving a high CBD content. In addition, a higher scCO2 consumption, which is related to longer processing time, was the most dominant factor affecting the extraction process exerting a significant effect, on both examined responses, yield and CBD content. A linear model equation was proposed to express both the yield and the CBD content as a function of independent variables.
PB  - Budapest : University of Technology and Economics
C3  - Book of Abstract / 19th European Meeting on Supercritical Fluids, Budapest, Hungary, 21-24 May 2023
T1  - Optimization of Cannabis sativa supercritical CO2 extraction using Design of experiments approach
SP  - 135
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6984
ER  - 

@conference{
author = "Lukić, Ivana and Stamenić, Marko and Tadić, Vanja and Skala, Dejan",
year = "2023",
abstract = "The high potential of industrial hemp (Cannabis sativa), mainly due to the high-quality fibers and bioactive compounds, coupled with the decriminalization and/or legalization in some regions of the world, led to the steady growth of scientific research over the past couple of decades [1, 2]. From an industrial point of view, cannabinoids, primarily non-psychotropic cannabidiol (CBD), are considered the most valuable compounds, as they possess a broad range of therapeutic properties, such as anxiolytic, neuroprotective, antibiotic, antiinflammatory activity, and anti-oxidant. The hemp industry has already adopted supercritical carbon dioxide scCO2 extraction as a well-established process since it is a promising ‘green’ alternative to conventional extraction methods, selective, with short processing time, and low impact on the environment.
In the present study, the preliminary analysis was performed to determine the influence of different
scCO2 densities based on the different combinations of process pressure and temperature on the kinetics of supercritical extraction of Cannabis sativa, extract yield and the chemical composition of obtained extracts. Experiments were performed at temperatures ranging from 40 to 60 °C and pressures ranging from 10 to 30 MPa. Prior to extraction, the plant material was grounded and submitted to decarboxylation process at 120 °C for 1 h, in order to transform cannabidiolic acid, which is the precursor present in the plant material, to CBD.
Experimental design for optimization plays an important role both in science and industry, because it allows evaluation of the effects of multiple factors and their interactions on one or more response variables. The present work was focused on the further optimization of scCO2 extraction with the aim to maximize the yield of extracts with high recoveries of CBD, by means of response surface methodology (RSM), based on a central composite design (CCD) using Design of Experiment software (Stat-Ease, Design Expert). For this purpose, independent variables were temperature (40-60 °C), pressure (10-30 MPa), and scCO2 consumption (20-60 gCO2/gplant).
The results showed that supercritical CO2 extraction enabled the achievement of up to 5% of total extract yield and 266–435 mg/g of CBD depending on the conditions. The high pressure and temperature, 30 MPa 60 °C, respectively, conditions that correspond to the highest density of the scCO2, generated the highest extract yield. On the contrary, lower pressure, corresponding to the lower density of CO2 was favourable for achieving a high CBD content. In addition, a higher scCO2 consumption, which is related to longer processing time, was the most dominant factor affecting the extraction process exerting a significant effect, on both examined responses, yield and CBD content. A linear model equation was proposed to express both the yield and the CBD content as a function of independent variables.",
publisher = "Budapest : University of Technology and Economics",
journal = "Book of Abstract / 19th European Meeting on Supercritical Fluids, Budapest, Hungary, 21-24 May 2023",
title = "Optimization of Cannabis sativa supercritical CO2 extraction using Design of experiments approach",
pages = "135",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6984"
}

Lukić, I., Stamenić, M., Tadić, V.,& Skala, D.. (2023). Optimization of Cannabis sativa supercritical CO2 extraction using Design of experiments approach. in Book of Abstract / 19th European Meeting on Supercritical Fluids, Budapest, Hungary, 21-24 May 2023
Budapest : University of Technology and Economics., 135.
https://hdl.handle.net/21.15107/rcub_technorep_6984

Lukić I, Stamenić M, Tadić V, Skala D. Optimization of Cannabis sativa supercritical CO2 extraction using Design of experiments approach. in Book of Abstract / 19th European Meeting on Supercritical Fluids, Budapest, Hungary, 21-24 May 2023. 2023;:135.
https://hdl.handle.net/21.15107/rcub_technorep_6984 .

Lukić, Ivana, Stamenić, Marko, Tadić, Vanja, Skala, Dejan, "Optimization of Cannabis sativa supercritical CO2 extraction using Design of experiments approach" in Book of Abstract / 19th European Meeting on Supercritical Fluids, Budapest, Hungary, 21-24 May 2023 (2023):135,
https://hdl.handle.net/21.15107/rcub_technorep_6984 .

TY  - CONF
AU  - Stamenić, Marko
AU  - Milovanović, Stoja
AU  - Kowalski, Rafał
AU  - Tyśkiewicz, Katarzyna
AU  - Konkol, Marcin
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6908
AB  - Investigations of the process of supercritical fluid extraction (SFE) from dandelion were so far mainly
focused on extractions from herb, leaves and root [1-3]. In the work presented here, the extraction process
from seeds was investigated on a laboratory and semi-industrial scale equipment. The milled plant material
with an average particle size of 0.41 ± 0.02 mm was used in all experiments. All SFEs were conducted with
carbon-dioxide in supercritical state (SCCO2) as a solvent on pressure of 450 bar and temperature of 60°C, as
these parameters were previously shown to be optimal for obtaining high-value extracts from dandelion
seeds [4]. Experiments were conducted on laboratory (dia.=7.7 cm, vol=0.56 l, CO2 flow=11 kg/h) and semiindustrial
(dia.=23.5 cm, vol.=397 l, CO2 flow=240 kg/h) scale equipment. On both scales several
experiments were conducted with initial mass of the plant material (Ms) being the only variable parameter:
laboratory scale Ms=26–104 g; semi-industrial scale Ms=2.2–8.8 kg. Fractions of extract were collected
during the extractions in order to obtain data on the kinetics of the SFE processes.
The results of the performed
experiments are depicted in Figure 1.
It can be seen that for both scales the
rate of SFE, as well as the final yield,
increases with increase of the mass
of plant material, indicating that with
the selected conditions, namely
flowrate of CO2, the residence time
was not long enough to allow
saturation of the CO2 in the first part
of the process, nor to enable
extraction of all available extract.
Similar trends on both scales can be
perceived as a good basis for further
investigations aiming at scale-up of the process of SFE from dandelion seeds. Experiments with larger
packed bed height, which would enable higher residence times, should be conducted in order to achieve
saturation at least in the first part of the SFE process. Mathematical models of the SFE process, supported by
models for estimation of the kinetic parameters, should be applied to gain full insight into the
phenomenology of the SFE process.
PB  - Budapest : University of Technology and Economics
C3  - Book of Abstract / 19th European Meeting on Supercritical Fluids, Budapest, Hungary, 21-24 May 2023
T1  - Supercritical fluid extraction from dandelion seeds
SP  - 136
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6908
ER  - 

@conference{
author = "Stamenić, Marko and Milovanović, Stoja and Kowalski, Rafał and Tyśkiewicz, Katarzyna and Konkol, Marcin",
year = "2023",
abstract = "Investigations of the process of supercritical fluid extraction (SFE) from dandelion were so far mainly
focused on extractions from herb, leaves and root [1-3]. In the work presented here, the extraction process
from seeds was investigated on a laboratory and semi-industrial scale equipment. The milled plant material
with an average particle size of 0.41 ± 0.02 mm was used in all experiments. All SFEs were conducted with
carbon-dioxide in supercritical state (SCCO2) as a solvent on pressure of 450 bar and temperature of 60°C, as
these parameters were previously shown to be optimal for obtaining high-value extracts from dandelion
seeds [4]. Experiments were conducted on laboratory (dia.=7.7 cm, vol=0.56 l, CO2 flow=11 kg/h) and semiindustrial
(dia.=23.5 cm, vol.=397 l, CO2 flow=240 kg/h) scale equipment. On both scales several
experiments were conducted with initial mass of the plant material (Ms) being the only variable parameter:
laboratory scale Ms=26–104 g; semi-industrial scale Ms=2.2–8.8 kg. Fractions of extract were collected
during the extractions in order to obtain data on the kinetics of the SFE processes.
The results of the performed
experiments are depicted in Figure 1.
It can be seen that for both scales the
rate of SFE, as well as the final yield,
increases with increase of the mass
of plant material, indicating that with
the selected conditions, namely
flowrate of CO2, the residence time
was not long enough to allow
saturation of the CO2 in the first part
of the process, nor to enable
extraction of all available extract.
Similar trends on both scales can be
perceived as a good basis for further
investigations aiming at scale-up of the process of SFE from dandelion seeds. Experiments with larger
packed bed height, which would enable higher residence times, should be conducted in order to achieve
saturation at least in the first part of the SFE process. Mathematical models of the SFE process, supported by
models for estimation of the kinetic parameters, should be applied to gain full insight into the
phenomenology of the SFE process.",
publisher = "Budapest : University of Technology and Economics",
journal = "Book of Abstract / 19th European Meeting on Supercritical Fluids, Budapest, Hungary, 21-24 May 2023",
title = "Supercritical fluid extraction from dandelion seeds",
pages = "136",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6908"
}

Stamenić, M., Milovanović, S., Kowalski, R., Tyśkiewicz, K.,& Konkol, M.. (2023). Supercritical fluid extraction from dandelion seeds. in Book of Abstract / 19th European Meeting on Supercritical Fluids, Budapest, Hungary, 21-24 May 2023
Budapest : University of Technology and Economics., 136.
https://hdl.handle.net/21.15107/rcub_technorep_6908

Stamenić M, Milovanović S, Kowalski R, Tyśkiewicz K, Konkol M. Supercritical fluid extraction from dandelion seeds. in Book of Abstract / 19th European Meeting on Supercritical Fluids, Budapest, Hungary, 21-24 May 2023. 2023;:136.
https://hdl.handle.net/21.15107/rcub_technorep_6908 .

Stamenić, Marko, Milovanović, Stoja, Kowalski, Rafał, Tyśkiewicz, Katarzyna, Konkol, Marcin, "Supercritical fluid extraction from dandelion seeds" in Book of Abstract / 19th European Meeting on Supercritical Fluids, Budapest, Hungary, 21-24 May 2023 (2023):136,
https://hdl.handle.net/21.15107/rcub_technorep_6908 .

TY  - CONF
AU  - Lukić, Ivana
AU  - Milovanović, Stoja
AU  - Stamenić, Marko
AU  - Tadić, Vanja
AU  - Skala, Dejan
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6985
AB  - Cannabis sativa is one of the oldest plant cultures, which has been used for centuries as a source of textile fibres, for paper production, as well as a source of bioactive compounds used in medicine. Although being restrained for a long time, its high potential, coupled with the progress in legislation and/or legalization in some regions of the world, led to the significant increase of scientific research towards isolation of cannabinoids, primarily a non-psychotropic cannabidiol (CBD), over the past couple decades. 
Beside cannabinoids, found only in cannabis plants, Cannabis sativa is characterized by very complex chemical composition with almost 500 constituents, including terpenes, flavonoids, phenolic compounds and fatty acids [2]. The co-presence of these numerous molecules in preparations obtained from Cannabis sativa may lead to a greater biological activity due to  the “entourage” or synergistic effect [3]. CBD has attracted considerable interest due to its numerous therapeutic properties and pharmacological activities [1]. However, cannabinoids are characterized by a low solubility in aqueous media and, as a consequence, a relatively poor bioavailability. Therefore, improvement of their stability, shelf-life and bioavailability, by incorporation into biocompatible carriers is still a challenge. 
As an answer to this issue, integrated process of supercritical fluid extraction (SFE) from Cannabis sativa and supercritical solvent impregnation (SSI) of obtained extract into starch aerogel was tested. Starch gels were prepared starting from hydrogel formed from an aqueous solution of cornstarch (1:10 w/v). During replacement of water with acetone, by successive increase of its concentration until 100%, acetogel was formed and further subjected to drying using supercritical CO2 (scCO2) at 45 °C and 8-10 MPa to obtain aerogel. For comparison, a part of acetogel was dried in the oven at 60 °C to obtain xerogel. Textural properties and morphology of prepared materials were determined by N2 adsorption–desorption isotherms and scanning electron microscopy (SEM) analysis. Aerogel with highest specific surface area of 208.6 m2/g, obtained at 45 °C and 10 MPa, was further used for extract impregnation. Integrated SFE-SSI process was performed at 40 °C and 10 MPa, conditions determined as optimal in the process of SFE of Cannabis sativa taking into account the yield of the obtained extract based on the composition, primarily regarding the CBD content. The amounts of impregnated extracts were 2.6 and 15.3% for xerogel and aerogel, respectively. The presence of the extract on the surface of impregnated samples was confirmed by FTIR analysis. Release study showed controlled release of extracts in PBS solution.
C3  - 2nd International Conference on Aerogels for Biomedical and Environmental Applications (AERoGELS2022), 29 June–1 July 2022, Athens, Greece, 2022
T1  - Starch aerogels loaded with Cannabis sativa extract using integrated process of supercritical CO2 extraction and impregnation
SP  - P-28
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6985
ER  - 

@conference{
author = "Lukić, Ivana and Milovanović, Stoja and Stamenić, Marko and Tadić, Vanja and Skala, Dejan",
year = "2022",
abstract = "Cannabis sativa is one of the oldest plant cultures, which has been used for centuries as a source of textile fibres, for paper production, as well as a source of bioactive compounds used in medicine. Although being restrained for a long time, its high potential, coupled with the progress in legislation and/or legalization in some regions of the world, led to the significant increase of scientific research towards isolation of cannabinoids, primarily a non-psychotropic cannabidiol (CBD), over the past couple decades. 
Beside cannabinoids, found only in cannabis plants, Cannabis sativa is characterized by very complex chemical composition with almost 500 constituents, including terpenes, flavonoids, phenolic compounds and fatty acids [2]. The co-presence of these numerous molecules in preparations obtained from Cannabis sativa may lead to a greater biological activity due to  the “entourage” or synergistic effect [3]. CBD has attracted considerable interest due to its numerous therapeutic properties and pharmacological activities [1]. However, cannabinoids are characterized by a low solubility in aqueous media and, as a consequence, a relatively poor bioavailability. Therefore, improvement of their stability, shelf-life and bioavailability, by incorporation into biocompatible carriers is still a challenge. 
As an answer to this issue, integrated process of supercritical fluid extraction (SFE) from Cannabis sativa and supercritical solvent impregnation (SSI) of obtained extract into starch aerogel was tested. Starch gels were prepared starting from hydrogel formed from an aqueous solution of cornstarch (1:10 w/v). During replacement of water with acetone, by successive increase of its concentration until 100%, acetogel was formed and further subjected to drying using supercritical CO2 (scCO2) at 45 °C and 8-10 MPa to obtain aerogel. For comparison, a part of acetogel was dried in the oven at 60 °C to obtain xerogel. Textural properties and morphology of prepared materials were determined by N2 adsorption–desorption isotherms and scanning electron microscopy (SEM) analysis. Aerogel with highest specific surface area of 208.6 m2/g, obtained at 45 °C and 10 MPa, was further used for extract impregnation. Integrated SFE-SSI process was performed at 40 °C and 10 MPa, conditions determined as optimal in the process of SFE of Cannabis sativa taking into account the yield of the obtained extract based on the composition, primarily regarding the CBD content. The amounts of impregnated extracts were 2.6 and 15.3% for xerogel and aerogel, respectively. The presence of the extract on the surface of impregnated samples was confirmed by FTIR analysis. Release study showed controlled release of extracts in PBS solution.",
journal = "2nd International Conference on Aerogels for Biomedical and Environmental Applications (AERoGELS2022), 29 June–1 July 2022, Athens, Greece, 2022",
title = "Starch aerogels loaded with Cannabis sativa extract using integrated process of supercritical CO2 extraction and impregnation",
pages = "P-28",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6985"
}

Lukić, I., Milovanović, S., Stamenić, M., Tadić, V.,& Skala, D.. (2022). Starch aerogels loaded with Cannabis sativa extract using integrated process of supercritical CO2 extraction and impregnation. in 2nd International Conference on Aerogels for Biomedical and Environmental Applications (AERoGELS2022), 29 June–1 July 2022, Athens, Greece, 2022, P-28.
https://hdl.handle.net/21.15107/rcub_technorep_6985

Lukić I, Milovanović S, Stamenić M, Tadić V, Skala D. Starch aerogels loaded with Cannabis sativa extract using integrated process of supercritical CO2 extraction and impregnation. in 2nd International Conference on Aerogels for Biomedical and Environmental Applications (AERoGELS2022), 29 June–1 July 2022, Athens, Greece, 2022. 2022;:P-28.
https://hdl.handle.net/21.15107/rcub_technorep_6985 .

Lukić, Ivana, Milovanović, Stoja, Stamenić, Marko, Tadić, Vanja, Skala, Dejan, "Starch aerogels loaded with Cannabis sativa extract using integrated process of supercritical CO2 extraction and impregnation" in 2nd International Conference on Aerogels for Biomedical and Environmental Applications (AERoGELS2022), 29 June–1 July 2022, Athens, Greece, 2022 (2022):P-28,
https://hdl.handle.net/21.15107/rcub_technorep_6985 .

TY  - JOUR
AU  - Milovanović, Stoja
AU  - Lukić, Ivana
AU  - Stamenić, Marko
AU  - Kamiński, Piotr
AU  - Florkowski, Grzegorz
AU  - Tyśkiewicz, Katarzyna
AU  - Konkol, Marcin
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5175
AB  - The present study was focused on improving the supercritical fluid extraction process dedicated to the recovery of highly valuable extract from Silybum marianum seeds. At first, the process was tested using supercritical CO2 at temperatures of 40 and 80 °C and pressures ranging from 100 to 300 bar. Obtained values were described using two literature models with acceptable agreement of experimental and calculated data. Further, the effect of the equipment design and process scale-up was investigated in four high-pressure units having volumes in the range from 280 mL to 40 L resulting in extraction yields up to 26 %. Based on the values of the parameters determined at the laboratory scale, the extraction process was successfully transferred to a semi-industrial scale. Finally, estimated costs for supercritical extract production in Poland for the year 2021 using a 1000 L high-pressure unit amounted to the price of 35.9 EUR per kg of extract.
PB  - Elsevier B.V.
T2  - Journal of Supercritical Fluids
T1  - The effect of equipment design and process scale-up on supercritical CO2 extraction: Case study for Silybum marianum seeds
SP  - 105676
VL  - 188
DO  - 10.1016/j.supflu.2022.105676
ER  - 

@article{
author = "Milovanović, Stoja and Lukić, Ivana and Stamenić, Marko and Kamiński, Piotr and Florkowski, Grzegorz and Tyśkiewicz, Katarzyna and Konkol, Marcin",
year = "2022",
abstract = "The present study was focused on improving the supercritical fluid extraction process dedicated to the recovery of highly valuable extract from Silybum marianum seeds. At first, the process was tested using supercritical CO2 at temperatures of 40 and 80 °C and pressures ranging from 100 to 300 bar. Obtained values were described using two literature models with acceptable agreement of experimental and calculated data. Further, the effect of the equipment design and process scale-up was investigated in four high-pressure units having volumes in the range from 280 mL to 40 L resulting in extraction yields up to 26 %. Based on the values of the parameters determined at the laboratory scale, the extraction process was successfully transferred to a semi-industrial scale. Finally, estimated costs for supercritical extract production in Poland for the year 2021 using a 1000 L high-pressure unit amounted to the price of 35.9 EUR per kg of extract.",
publisher = "Elsevier B.V.",
journal = "Journal of Supercritical Fluids",
title = "The effect of equipment design and process scale-up on supercritical CO2 extraction: Case study for Silybum marianum seeds",
pages = "105676",
volume = "188",
doi = "10.1016/j.supflu.2022.105676"
}

Milovanović, S., Lukić, I., Stamenić, M., Kamiński, P., Florkowski, G., Tyśkiewicz, K.,& Konkol, M.. (2022). The effect of equipment design and process scale-up on supercritical CO2 extraction: Case study for Silybum marianum seeds. in Journal of Supercritical Fluids
Elsevier B.V.., 188, 105676.
https://doi.org/10.1016/j.supflu.2022.105676

Milovanović S, Lukić I, Stamenić M, Kamiński P, Florkowski G, Tyśkiewicz K, Konkol M. The effect of equipment design and process scale-up on supercritical CO2 extraction: Case study for Silybum marianum seeds. in Journal of Supercritical Fluids. 2022;188:105676.
doi:10.1016/j.supflu.2022.105676 .

Milovanović, Stoja, Lukić, Ivana, Stamenić, Marko, Kamiński, Piotr, Florkowski, Grzegorz, Tyśkiewicz, Katarzyna, Konkol, Marcin, "The effect of equipment design and process scale-up on supercritical CO2 extraction: Case study for Silybum marianum seeds" in Journal of Supercritical Fluids, 188 (2022):105676,
https://doi.org/10.1016/j.supflu.2022.105676 . .

TY  - JOUR
AU  - Dali, Imen
AU  - Aydi, Abdelkarim
AU  - Stamenić, Marko
AU  - Kolsi, Lioua
AU  - Ghachem, Kaouther
AU  - Zizovic, Irena
AU  - Manef, Abderrabba
AU  - Delgado, Daniel R.
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4977
AB  - The olive growing in Tunisia has an economic dominance and agricultural importance. However, the huge extraction of olive oil generates a large quantity of olive mill wastewater (OMW), which is discharged to the surroundings. The highly polluting potential (organic load) of OMW threatens the environment and requires an urgent solution. Supercritical fluid extraction (SFE) is a green extraction method that can be applied to purify OMW and, at the same time, to isolate a high quality oil from this wastewater. In order to explore and to valorize the compositions of Olive mill wastewater (OMW), extraction in different solvents (supercritical CO2, hexane) was carried out and chemical composition of the extracted oils were established by GC-FID. The Tunisia OMW were collected from two different zones namely Sousse and Sfax. In this work, we have investigated the effects pressure (P) and temperature (T) on the yield and the quality of oil. The suitable conditions for the extraction of oil from lyophilized OMW by Super-critical carbon dioxide (SC-CO2) were found to be the pressure of 30 MPa and the temperature of 60 degrees C. In order to simulate the process, the model of broken and intact cells (Sovova's model) was applied. The model well represented the experimental data.
T2  - Alexandria Engineering Journal
T1  - Extraction of lyophilized olive mill wastewater using supercritical CO2 processes
EP  - 246
IS  - 1
SP  - 237
VL  - 61
DO  - 10.1016/j.aej.2021.04.0881110-0168
ER  - 

@article{
author = "Dali, Imen and Aydi, Abdelkarim and Stamenić, Marko and Kolsi, Lioua and Ghachem, Kaouther and Zizovic, Irena and Manef, Abderrabba and Delgado, Daniel R.",
year = "2022",
abstract = "The olive growing in Tunisia has an economic dominance and agricultural importance. However, the huge extraction of olive oil generates a large quantity of olive mill wastewater (OMW), which is discharged to the surroundings. The highly polluting potential (organic load) of OMW threatens the environment and requires an urgent solution. Supercritical fluid extraction (SFE) is a green extraction method that can be applied to purify OMW and, at the same time, to isolate a high quality oil from this wastewater. In order to explore and to valorize the compositions of Olive mill wastewater (OMW), extraction in different solvents (supercritical CO2, hexane) was carried out and chemical composition of the extracted oils were established by GC-FID. The Tunisia OMW were collected from two different zones namely Sousse and Sfax. In this work, we have investigated the effects pressure (P) and temperature (T) on the yield and the quality of oil. The suitable conditions for the extraction of oil from lyophilized OMW by Super-critical carbon dioxide (SC-CO2) were found to be the pressure of 30 MPa and the temperature of 60 degrees C. In order to simulate the process, the model of broken and intact cells (Sovova's model) was applied. The model well represented the experimental data.",
journal = "Alexandria Engineering Journal",
title = "Extraction of lyophilized olive mill wastewater using supercritical CO2 processes",
pages = "246-237",
number = "1",
volume = "61",
doi = "10.1016/j.aej.2021.04.0881110-0168"
}

Dali, I., Aydi, A., Stamenić, M., Kolsi, L., Ghachem, K., Zizovic, I., Manef, A.,& Delgado, D. R.. (2022). Extraction of lyophilized olive mill wastewater using supercritical CO2 processes. in Alexandria Engineering Journal, 61(1), 237-246.
https://doi.org/10.1016/j.aej.2021.04.0881110-0168

Dali I, Aydi A, Stamenić M, Kolsi L, Ghachem K, Zizovic I, Manef A, Delgado DR. Extraction of lyophilized olive mill wastewater using supercritical CO2 processes. in Alexandria Engineering Journal. 2022;61(1):237-246.
doi:10.1016/j.aej.2021.04.0881110-0168 .

Dali, Imen, Aydi, Abdelkarim, Stamenić, Marko, Kolsi, Lioua, Ghachem, Kaouther, Zizovic, Irena, Manef, Abderrabba, Delgado, Daniel R., "Extraction of lyophilized olive mill wastewater using supercritical CO2 processes" in Alexandria Engineering Journal, 61, no. 1 (2022):237-246,
https://doi.org/10.1016/j.aej.2021.04.0881110-0168 . .

TY  - JOUR
AU  - Zivanić, Ljiljana
AU  - Stamenić, Marko
AU  - Todić, Branislav
AU  - Bukur, Dragomir B.
AU  - Nikačević, Nikola
PY  - 2019
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4246
AB  - Predictions of vapor liquid equilibrium for Fischer-Tropsch mixtures were com-pared using the classical Soave-Redlich-Kwong (SRK) and cubic-plus-asso-ciation (CPA) equations of state. The performance of the two equations of state was evaluated based on comparison with results from eight sets of exp-erimental runs in which different process conditions (pressure, reactants feed ratio, space velocity) were used. Flash calculations were used to determine the phase split at defined process conditions, whereas the phase equilibrium was defined utilizing the concept of equal fugacities in the vapor and the liquid phase for all components. A total of 75 components were considered in the reaction mixture: CO, H-2, H2O, CO2, C-1-C-57 paraffins and C-2-C-15 olefins. All calculations were performed in MATLAB. The results showed that both equat-ions of state had similar performance regarding the hydrocarbons, whereas CPA gave better results with inorganic components and SRK with prediction of the composition of the liquid phase. Computational time for CPA was sub-stantially (100 times with the CPU used) higher than that for SRK. Overall, the use of CPA did not improve VLE prediction for FTS systems significantly enough to be recommended for use in FTS reactor models.
PB  - Savez hemijskih inženjera, Beograd
T2  - Chemical Industry & Chemical Engineering Quarterly
T1  - Comparison of cubic-plus-association and soave-redlich-kwong equations of state for prediction of vapor-liquid equilibrium of fischer-tropsch reaction mixture
EP  - 76
IS  - 1
SP  - 67
VL  - 25
DO  - 10.2298/CICEQ180403018Z
ER  - 

@article{
author = "Zivanić, Ljiljana and Stamenić, Marko and Todić, Branislav and Bukur, Dragomir B. and Nikačević, Nikola",
year = "2019",
abstract = "Predictions of vapor liquid equilibrium for Fischer-Tropsch mixtures were com-pared using the classical Soave-Redlich-Kwong (SRK) and cubic-plus-asso-ciation (CPA) equations of state. The performance of the two equations of state was evaluated based on comparison with results from eight sets of exp-erimental runs in which different process conditions (pressure, reactants feed ratio, space velocity) were used. Flash calculations were used to determine the phase split at defined process conditions, whereas the phase equilibrium was defined utilizing the concept of equal fugacities in the vapor and the liquid phase for all components. A total of 75 components were considered in the reaction mixture: CO, H-2, H2O, CO2, C-1-C-57 paraffins and C-2-C-15 olefins. All calculations were performed in MATLAB. The results showed that both equat-ions of state had similar performance regarding the hydrocarbons, whereas CPA gave better results with inorganic components and SRK with prediction of the composition of the liquid phase. Computational time for CPA was sub-stantially (100 times with the CPU used) higher than that for SRK. Overall, the use of CPA did not improve VLE prediction for FTS systems significantly enough to be recommended for use in FTS reactor models.",
publisher = "Savez hemijskih inženjera, Beograd",
journal = "Chemical Industry & Chemical Engineering Quarterly",
title = "Comparison of cubic-plus-association and soave-redlich-kwong equations of state for prediction of vapor-liquid equilibrium of fischer-tropsch reaction mixture",
pages = "76-67",
number = "1",
volume = "25",
doi = "10.2298/CICEQ180403018Z"
}

Zivanić, L., Stamenić, M., Todić, B., Bukur, D. B.,& Nikačević, N.. (2019). Comparison of cubic-plus-association and soave-redlich-kwong equations of state for prediction of vapor-liquid equilibrium of fischer-tropsch reaction mixture. in Chemical Industry & Chemical Engineering Quarterly
Savez hemijskih inženjera, Beograd., 25(1), 67-76.
https://doi.org/10.2298/CICEQ180403018Z

Zivanić L, Stamenić M, Todić B, Bukur DB, Nikačević N. Comparison of cubic-plus-association and soave-redlich-kwong equations of state for prediction of vapor-liquid equilibrium of fischer-tropsch reaction mixture. in Chemical Industry & Chemical Engineering Quarterly. 2019;25(1):67-76.
doi:10.2298/CICEQ180403018Z .

Zivanić, Ljiljana, Stamenić, Marko, Todić, Branislav, Bukur, Dragomir B., Nikačević, Nikola, "Comparison of cubic-plus-association and soave-redlich-kwong equations of state for prediction of vapor-liquid equilibrium of fischer-tropsch reaction mixture" in Chemical Industry & Chemical Engineering Quarterly, 25, no. 1 (2019):67-76,
https://doi.org/10.2298/CICEQ180403018Z . .

TY  - JOUR
AU  - Stamenić, Marko
AU  - Dikić, Vladimir
AU  - Mandić, Miloš
AU  - Todić, Branislav
AU  - Bukur, Dragomir B.
AU  - Nikačević, Nikola
PY  - 2018
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3964
AB  - Our previously developed mathematical model is used for parametric sensitivity and optimization study of conventional and milliscale fixed-bed reactors (FBRs) for Fischer-Tropsch synthesis (FTS). Five indicators are chosen to analyze the influence of eight parameters on the FBRs performance. The results show the scale of the effects caused by changing single parameter values and highlight the most important ones. Subsequently, the model is used to perform a rigorous multivariable optimization of the FBRs performance in the steady state. Three optimization functions are used, depicting different reactor costs. Four design parameters (tube length and diameter, particle diameter, and catalyst layer thickness) and five operating parameters (inlet and wall temperature, inlet pressure, H-2/CO ratio, velocity) are optimized simultaneously. The results indicate that optimal results, in terms of reactor design and operating parameters and FBR performance, highly depend on the selected objective function and values of constrained parameters (especially methane selectivity and the partial pressure of water).
PB  - Amer Chemical Soc, Washington
T2  - Industrial & Engineering Chemistry Research
T1  - Multiscale and Multiphase Model of Fixed-Bed Reactors for Fischer-Tropsch Synthesis: Optimization Study
EP  - 3162
IS  - 9
SP  - 3149
VL  - 57
DO  - 10.1021/acs.iecr.7b04914
ER  - 

@article{
author = "Stamenić, Marko and Dikić, Vladimir and Mandić, Miloš and Todić, Branislav and Bukur, Dragomir B. and Nikačević, Nikola",
year = "2018",
abstract = "Our previously developed mathematical model is used for parametric sensitivity and optimization study of conventional and milliscale fixed-bed reactors (FBRs) for Fischer-Tropsch synthesis (FTS). Five indicators are chosen to analyze the influence of eight parameters on the FBRs performance. The results show the scale of the effects caused by changing single parameter values and highlight the most important ones. Subsequently, the model is used to perform a rigorous multivariable optimization of the FBRs performance in the steady state. Three optimization functions are used, depicting different reactor costs. Four design parameters (tube length and diameter, particle diameter, and catalyst layer thickness) and five operating parameters (inlet and wall temperature, inlet pressure, H-2/CO ratio, velocity) are optimized simultaneously. The results indicate that optimal results, in terms of reactor design and operating parameters and FBR performance, highly depend on the selected objective function and values of constrained parameters (especially methane selectivity and the partial pressure of water).",
publisher = "Amer Chemical Soc, Washington",
journal = "Industrial & Engineering Chemistry Research",
title = "Multiscale and Multiphase Model of Fixed-Bed Reactors for Fischer-Tropsch Synthesis: Optimization Study",
pages = "3162-3149",
number = "9",
volume = "57",
doi = "10.1021/acs.iecr.7b04914"
}

Stamenić, M., Dikić, V., Mandić, M., Todić, B., Bukur, D. B.,& Nikačević, N.. (2018). Multiscale and Multiphase Model of Fixed-Bed Reactors for Fischer-Tropsch Synthesis: Optimization Study. in Industrial & Engineering Chemistry Research
Amer Chemical Soc, Washington., 57(9), 3149-3162.
https://doi.org/10.1021/acs.iecr.7b04914

Stamenić M, Dikić V, Mandić M, Todić B, Bukur DB, Nikačević N. Multiscale and Multiphase Model of Fixed-Bed Reactors for Fischer-Tropsch Synthesis: Optimization Study. in Industrial & Engineering Chemistry Research. 2018;57(9):3149-3162.
doi:10.1021/acs.iecr.7b04914 .

Stamenić, Marko, Dikić, Vladimir, Mandić, Miloš, Todić, Branislav, Bukur, Dragomir B., Nikačević, Nikola, "Multiscale and Multiphase Model of Fixed-Bed Reactors for Fischer-Tropsch Synthesis: Optimization Study" in Industrial & Engineering Chemistry Research, 57, no. 9 (2018):3149-3162,
https://doi.org/10.1021/acs.iecr.7b04914 . .

TY  - JOUR
AU  - Pajnik, Jelena
AU  - Stamenić, Marko
AU  - Radetić, Maja
AU  - Tomanović, Snežana
AU  - Sukara, Ratko
AU  - Mihaljica, Darko
AU  - Žižović, Irena
PY  - 2017
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3574
AB  - This study discusses a possibility of cotton fabric impregnation with pyrethrum extract as a tick repellent in supercritical carbon dioxide. A solubility of pyrethrum extract in supercritical carbon dioxide has been determined at 35 and 40 degrees C and pressures ranging from 8 to 20 MPa. The highest solubility of 48.69 kg/m(3) was obtained at temperature of 35 degrees C and pressure of 20 MPa. Density -based equations of Chrastil, Adachi-Lu and del Valle-Aguilera were employed to correlate the experimental data. Based on the solubility data, conditions for the impregnation of cotton fabric were selected. Conditions at 40 degrees C and 8 MPa provided targeted quantities of the impregnated extract of 0.5% and 1% after 1 h and 2 h of the impregnation, respectively. The presence of pyrethrins on the cotton fabric's surface was confirmed by FTIR analysis. Repellent activity of both impregnated cotton fabrics (pyrethrum extract contents of 0.5% and 1%) was proven against ticks.
PB  - Elsevier Science Bv, Amsterdam
T2  - Journal of Supercritical Fluids
T1  - Impregnation of cotton fabric with pyrethrum extract in supercritical carbon dioxide
EP  - 72
SP  - 66
VL  - 128
DO  - 10.1016/j.supflu.2017.05.006
ER  - 

@article{
author = "Pajnik, Jelena and Stamenić, Marko and Radetić, Maja and Tomanović, Snežana and Sukara, Ratko and Mihaljica, Darko and Žižović, Irena",
year = "2017",
abstract = "This study discusses a possibility of cotton fabric impregnation with pyrethrum extract as a tick repellent in supercritical carbon dioxide. A solubility of pyrethrum extract in supercritical carbon dioxide has been determined at 35 and 40 degrees C and pressures ranging from 8 to 20 MPa. The highest solubility of 48.69 kg/m(3) was obtained at temperature of 35 degrees C and pressure of 20 MPa. Density -based equations of Chrastil, Adachi-Lu and del Valle-Aguilera were employed to correlate the experimental data. Based on the solubility data, conditions for the impregnation of cotton fabric were selected. Conditions at 40 degrees C and 8 MPa provided targeted quantities of the impregnated extract of 0.5% and 1% after 1 h and 2 h of the impregnation, respectively. The presence of pyrethrins on the cotton fabric's surface was confirmed by FTIR analysis. Repellent activity of both impregnated cotton fabrics (pyrethrum extract contents of 0.5% and 1%) was proven against ticks.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Journal of Supercritical Fluids",
title = "Impregnation of cotton fabric with pyrethrum extract in supercritical carbon dioxide",
pages = "72-66",
volume = "128",
doi = "10.1016/j.supflu.2017.05.006"
}

Pajnik, J., Stamenić, M., Radetić, M., Tomanović, S., Sukara, R., Mihaljica, D.,& Žižović, I.. (2017). Impregnation of cotton fabric with pyrethrum extract in supercritical carbon dioxide. in Journal of Supercritical Fluids
Elsevier Science Bv, Amsterdam., 128, 66-72.
https://doi.org/10.1016/j.supflu.2017.05.006

Pajnik J, Stamenić M, Radetić M, Tomanović S, Sukara R, Mihaljica D, Žižović I. Impregnation of cotton fabric with pyrethrum extract in supercritical carbon dioxide. in Journal of Supercritical Fluids. 2017;128:66-72.
doi:10.1016/j.supflu.2017.05.006 .

Pajnik, Jelena, Stamenić, Marko, Radetić, Maja, Tomanović, Snežana, Sukara, Ratko, Mihaljica, Darko, Žižović, Irena, "Impregnation of cotton fabric with pyrethrum extract in supercritical carbon dioxide" in Journal of Supercritical Fluids, 128 (2017):66-72,
https://doi.org/10.1016/j.supflu.2017.05.006 . .

TY  - JOUR
AU  - Stamenić, Marko
AU  - Dikić, Vladimir
AU  - Mandić, Miloš
AU  - Todić, Branislav
AU  - Bukur, Dragomir B.
AU  - Nikačević, Nikola
PY  - 2017
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3542
AB  - A multiphase fixed-bed reactor (FBR) model for Fischer Tropsch Synthesis has been developed. A high level of details is considered for description of the phenomena on the reactor and particle scale. Detailed kinetics is used, with parameters estimated from experiments with a cobalt-based catalyst. Model robustness has been validated using literature data. Performance analysis was made for a conventional scale FBR with egg-shell distribution of catalyst and a millimeter-scale FBR with small particles and uniform distribution. In both cases, diffusion limitations are almost eliminated due to use of small diffusion lengths. For similar qualitative results, a milli-scaled design would result in a significantly lower reactor volume, but the capital costs could be high due to large wall area and a vast number of tubes. Heat removal is efficient in both cases, and pressure drop in the milli-scale reactor is low due to the use of a shorter bed and lower velocity.
PB  - Amer Chemical Soc, Washington
T2  - Industrial & Engineering Chemistry Research
T1  - Multiscale and Multiphase Model of Fixed Bed Reactors for Fischer-Tropsch Synthesis: Intensification Possibilities Study
EP  - 9979
IS  - 36
SP  - 9964
VL  - 56
DO  - 10.1021/acs.iecr.7b02467
ER  - 

@article{
author = "Stamenić, Marko and Dikić, Vladimir and Mandić, Miloš and Todić, Branislav and Bukur, Dragomir B. and Nikačević, Nikola",
year = "2017",
abstract = "A multiphase fixed-bed reactor (FBR) model for Fischer Tropsch Synthesis has been developed. A high level of details is considered for description of the phenomena on the reactor and particle scale. Detailed kinetics is used, with parameters estimated from experiments with a cobalt-based catalyst. Model robustness has been validated using literature data. Performance analysis was made for a conventional scale FBR with egg-shell distribution of catalyst and a millimeter-scale FBR with small particles and uniform distribution. In both cases, diffusion limitations are almost eliminated due to use of small diffusion lengths. For similar qualitative results, a milli-scaled design would result in a significantly lower reactor volume, but the capital costs could be high due to large wall area and a vast number of tubes. Heat removal is efficient in both cases, and pressure drop in the milli-scale reactor is low due to the use of a shorter bed and lower velocity.",
publisher = "Amer Chemical Soc, Washington",
journal = "Industrial & Engineering Chemistry Research",
title = "Multiscale and Multiphase Model of Fixed Bed Reactors for Fischer-Tropsch Synthesis: Intensification Possibilities Study",
pages = "9979-9964",
number = "36",
volume = "56",
doi = "10.1021/acs.iecr.7b02467"
}

Stamenić, M., Dikić, V., Mandić, M., Todić, B., Bukur, D. B.,& Nikačević, N.. (2017). Multiscale and Multiphase Model of Fixed Bed Reactors for Fischer-Tropsch Synthesis: Intensification Possibilities Study. in Industrial & Engineering Chemistry Research
Amer Chemical Soc, Washington., 56(36), 9964-9979.
https://doi.org/10.1021/acs.iecr.7b02467

Stamenić M, Dikić V, Mandić M, Todić B, Bukur DB, Nikačević N. Multiscale and Multiphase Model of Fixed Bed Reactors for Fischer-Tropsch Synthesis: Intensification Possibilities Study. in Industrial & Engineering Chemistry Research. 2017;56(36):9964-9979.
doi:10.1021/acs.iecr.7b02467 .

Stamenić, Marko, Dikić, Vladimir, Mandić, Miloš, Todić, Branislav, Bukur, Dragomir B., Nikačević, Nikola, "Multiscale and Multiphase Model of Fixed Bed Reactors for Fischer-Tropsch Synthesis: Intensification Possibilities Study" in Industrial & Engineering Chemistry Research, 56, no. 36 (2017):9964-9979,
https://doi.org/10.1021/acs.iecr.7b02467 . .

TY  - CHAP
AU  - Meyer, Florian
AU  - Stamenić, Marko
AU  - Žižović, Irena
AU  - Eggers, Rudolf
PY  - 2017
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3489
AB  - A new trend in mathematical modeling of supercritical extraction process from plant material is presented. The most of the previously published models considered properties of the fixed bed of plant material during the extraction to be constant. This assumption might be quite true in the case of plant materials with relatively low quantity of extractables. However, the fixed bed property change may occur in the case of extraction from material with high quantities of extractables. Recently, mathematical model which took into account particle density, bed porosity and particle diameter as variables was derived. In this chapter, the model is further improved by introducing variable solubility of the extract in supercritical fluid. On the basis of new experimental results on the binary equilibrium of the system supercritical fluid - extract present in solid, a relationship between solubility in supercritical fluid and oil content in solid was established. The new model was derived which took into account particle density, bed porosity, particle diameter and solubility in supercritical fluid as variables. The model was verified on results from experiments in which the kinetics of supercritical extraction from rapseed was analyzed. Parameters of the model showed expected behaviour with respect to the change of particle size. Further analysis, shown in this chapter, showed that, if the dependency of solubility from the concentration in the solid phase is not accounted for, the model tends to overestimate the yield of the extraction. The new model is so far, the most realistic model and one of the most demanding models regarding experimental work and mathematical tools for the description of mass transfer in fixed beds.
T2  - Mathematical Research Summaries
T1  - Advances in mathematical modeling of supercritical extraction processes
EP  - 102
SP  - 101
VL  - 2
UR  - https://hdl.handle.net/21.15107/rcub_technorep_3489
ER  - 

@inbook{
author = "Meyer, Florian and Stamenić, Marko and Žižović, Irena and Eggers, Rudolf",
year = "2017",
abstract = "A new trend in mathematical modeling of supercritical extraction process from plant material is presented. The most of the previously published models considered properties of the fixed bed of plant material during the extraction to be constant. This assumption might be quite true in the case of plant materials with relatively low quantity of extractables. However, the fixed bed property change may occur in the case of extraction from material with high quantities of extractables. Recently, mathematical model which took into account particle density, bed porosity and particle diameter as variables was derived. In this chapter, the model is further improved by introducing variable solubility of the extract in supercritical fluid. On the basis of new experimental results on the binary equilibrium of the system supercritical fluid - extract present in solid, a relationship between solubility in supercritical fluid and oil content in solid was established. The new model was derived which took into account particle density, bed porosity, particle diameter and solubility in supercritical fluid as variables. The model was verified on results from experiments in which the kinetics of supercritical extraction from rapseed was analyzed. Parameters of the model showed expected behaviour with respect to the change of particle size. Further analysis, shown in this chapter, showed that, if the dependency of solubility from the concentration in the solid phase is not accounted for, the model tends to overestimate the yield of the extraction. The new model is so far, the most realistic model and one of the most demanding models regarding experimental work and mathematical tools for the description of mass transfer in fixed beds.",
journal = "Mathematical Research Summaries",
booktitle = "Advances in mathematical modeling of supercritical extraction processes",
pages = "102-101",
volume = "2",
url = "https://hdl.handle.net/21.15107/rcub_technorep_3489"
}

Meyer, F., Stamenić, M., Žižović, I.,& Eggers, R.. (2017). Advances in mathematical modeling of supercritical extraction processes. in Mathematical Research Summaries, 2, 101-102.
https://hdl.handle.net/21.15107/rcub_technorep_3489

Meyer F, Stamenić M, Žižović I, Eggers R. Advances in mathematical modeling of supercritical extraction processes. in Mathematical Research Summaries. 2017;2:101-102.
https://hdl.handle.net/21.15107/rcub_technorep_3489 .

Meyer, Florian, Stamenić, Marko, Žižović, Irena, Eggers, Rudolf, "Advances in mathematical modeling of supercritical extraction processes" in Mathematical Research Summaries, 2 (2017):101-102,
https://hdl.handle.net/21.15107/rcub_technorep_3489 .

TY  - JOUR
AU  - Ivanović, Jasna
AU  - Knauer, Sandra
AU  - Fanovich, Alejandra
AU  - Milovanović, Stoja
AU  - Stamenić, Marko
AU  - Jaeger, Philip
AU  - Žižović, Irena
AU  - Eggers, Rudolf
PY  - 2016
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3407
AB  - The potential of supercritical carbon dioxide (scCO(2)) processing of polycaprolactone (Pa) and polycaprolactone-hydroxyapatite (PCL-HA) composites for obtaining functional porous scaffolds at moderate temperatures (35-40 degrees C) was analyzed and quantified. The effect of scCO(2) sorption kinetics on the swelling, foam morphology and thermal behavior of the PCL and PCL-HA composites was studied. Sorption isotherms were determined using magnetic suspension balance at 10-30 MPa and 35-40 degrees C. Influence of the pressure, HA amount (10-20%) and procedure for HA powder preparation on the sorption kinetics was discussed. Supercritical solvent impregnation (SSI) of PCL and PCL-HA with thymol was investigated as environmentally friendly way to produce functional scaffolds with controlled microstructure. Moderately high pressures (13-17 MPa) and 10% of HA were proven to be favorable for creation of the PCL scaffolds with satisfying foam microstructure (mean pore size similar to 200-300 mu m), filler distribution and thymol impregnation yields (12-18%). This was due to the satisfactory high scCO(2) solubility (0.25-0.30 g/g) and mass transfer rate (similar to 10(-10) m(2)/s) in the polymer phase as well as its great plasticizing effect (-Delta T-m = 26.4-27.4 degrees C). Higher saturation of the polymer phase with scCO(2) (0.30-0.55 g/g), filler amount (20%) and thymol solubility in scCO(2) ( gt 5.5 g/kg) at pressures above 20 MPa resulted in decreased mass transfer rate and SSI efficiency.
PB  - Elsevier, Amsterdam
T2  - Journal of Supercritical Fluids
T1  - Supercritical CO2 sorption kinetics and thymol impregnation of PCL and PCL-HA
EP  - 498
SP  - 486
VL  - 107
DO  - 10.1016/j.supflu.2015.07.001
ER  - 

@article{
author = "Ivanović, Jasna and Knauer, Sandra and Fanovich, Alejandra and Milovanović, Stoja and Stamenić, Marko and Jaeger, Philip and Žižović, Irena and Eggers, Rudolf",
year = "2016",
abstract = "The potential of supercritical carbon dioxide (scCO(2)) processing of polycaprolactone (Pa) and polycaprolactone-hydroxyapatite (PCL-HA) composites for obtaining functional porous scaffolds at moderate temperatures (35-40 degrees C) was analyzed and quantified. The effect of scCO(2) sorption kinetics on the swelling, foam morphology and thermal behavior of the PCL and PCL-HA composites was studied. Sorption isotherms were determined using magnetic suspension balance at 10-30 MPa and 35-40 degrees C. Influence of the pressure, HA amount (10-20%) and procedure for HA powder preparation on the sorption kinetics was discussed. Supercritical solvent impregnation (SSI) of PCL and PCL-HA with thymol was investigated as environmentally friendly way to produce functional scaffolds with controlled microstructure. Moderately high pressures (13-17 MPa) and 10% of HA were proven to be favorable for creation of the PCL scaffolds with satisfying foam microstructure (mean pore size similar to 200-300 mu m), filler distribution and thymol impregnation yields (12-18%). This was due to the satisfactory high scCO(2) solubility (0.25-0.30 g/g) and mass transfer rate (similar to 10(-10) m(2)/s) in the polymer phase as well as its great plasticizing effect (-Delta T-m = 26.4-27.4 degrees C). Higher saturation of the polymer phase with scCO(2) (0.30-0.55 g/g), filler amount (20%) and thymol solubility in scCO(2) ( gt 5.5 g/kg) at pressures above 20 MPa resulted in decreased mass transfer rate and SSI efficiency.",
publisher = "Elsevier, Amsterdam",
journal = "Journal of Supercritical Fluids",
title = "Supercritical CO2 sorption kinetics and thymol impregnation of PCL and PCL-HA",
pages = "498-486",
volume = "107",
doi = "10.1016/j.supflu.2015.07.001"
}

Ivanović, J., Knauer, S., Fanovich, A., Milovanović, S., Stamenić, M., Jaeger, P., Žižović, I.,& Eggers, R.. (2016). Supercritical CO2 sorption kinetics and thymol impregnation of PCL and PCL-HA. in Journal of Supercritical Fluids
Elsevier, Amsterdam., 107, 486-498.
https://doi.org/10.1016/j.supflu.2015.07.001

Ivanović J, Knauer S, Fanovich A, Milovanović S, Stamenić M, Jaeger P, Žižović I, Eggers R. Supercritical CO2 sorption kinetics and thymol impregnation of PCL and PCL-HA. in Journal of Supercritical Fluids. 2016;107:486-498.
doi:10.1016/j.supflu.2015.07.001 .

Ivanović, Jasna, Knauer, Sandra, Fanovich, Alejandra, Milovanović, Stoja, Stamenić, Marko, Jaeger, Philip, Žižović, Irena, Eggers, Rudolf, "Supercritical CO2 sorption kinetics and thymol impregnation of PCL and PCL-HA" in Journal of Supercritical Fluids, 107 (2016):486-498,
https://doi.org/10.1016/j.supflu.2015.07.001 . .

TY  - JOUR
AU  - Bogdanović, Aleksandra
AU  - Tadić, Vanja
AU  - Stamenić, Marko
AU  - Petrović, Slobodan
AU  - Skala, Dejan
PY  - 2016
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3405
AB  - Pretreatment of fenugreek (Trigonella foenum-graecum L, Fabaceae) seeds was achieved using the step of defatting and acid hydrolysis. The obtained hydro-isolate (HI) was used as initial feed for diosgenin isolation by supercritical CO2 extraction (SC-CO2). Preliminary tests performed at conditions resulting in SC-CO2 density ranging from 800 to 920 kg/m(3) indicated that at some pressure, temperature as well as consumption of supercritical fluids the optimal working conditions for diosgenin isolation could be determined. For this purpose, the following range of working conditions of SC-CO2 were tested by using Central Composite Rotatable Design (CCRD) and Response Surface Methodology (RSM): pressure from 16.6 to 33.4 MPa, temperature from 30 to 50 degrees C and consumption of SC-CO2 from 11.6 to 28.4 g(co2)/g(dm). The result of this investigation indicated that maximum yield of 0.81 mg diosgenin from 1 g of fenugreek seeds on dry basis (about 1.3% of total extract) at 24.6 MPa, 43.5 degrees C, and 20.87 g(co2)/g(dm) could be obtained.
PB  - Elsevier Science Bv, Amsterdam
T2  - Journal of Supercritical Fluids
T1  - Supercritical carbon dioxide extraction of Trigonella foenum-graecum L. seeds: Process optimization using response surface methodology
EP  - 50
SP  - 44
VL  - 107
DO  - 10.1016/j.supflu.2015.08.003
ER  - 

@article{
author = "Bogdanović, Aleksandra and Tadić, Vanja and Stamenić, Marko and Petrović, Slobodan and Skala, Dejan",
year = "2016",
abstract = "Pretreatment of fenugreek (Trigonella foenum-graecum L, Fabaceae) seeds was achieved using the step of defatting and acid hydrolysis. The obtained hydro-isolate (HI) was used as initial feed for diosgenin isolation by supercritical CO2 extraction (SC-CO2). Preliminary tests performed at conditions resulting in SC-CO2 density ranging from 800 to 920 kg/m(3) indicated that at some pressure, temperature as well as consumption of supercritical fluids the optimal working conditions for diosgenin isolation could be determined. For this purpose, the following range of working conditions of SC-CO2 were tested by using Central Composite Rotatable Design (CCRD) and Response Surface Methodology (RSM): pressure from 16.6 to 33.4 MPa, temperature from 30 to 50 degrees C and consumption of SC-CO2 from 11.6 to 28.4 g(co2)/g(dm). The result of this investigation indicated that maximum yield of 0.81 mg diosgenin from 1 g of fenugreek seeds on dry basis (about 1.3% of total extract) at 24.6 MPa, 43.5 degrees C, and 20.87 g(co2)/g(dm) could be obtained.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Journal of Supercritical Fluids",
title = "Supercritical carbon dioxide extraction of Trigonella foenum-graecum L. seeds: Process optimization using response surface methodology",
pages = "50-44",
volume = "107",
doi = "10.1016/j.supflu.2015.08.003"
}

Bogdanović, A., Tadić, V., Stamenić, M., Petrović, S.,& Skala, D.. (2016). Supercritical carbon dioxide extraction of Trigonella foenum-graecum L. seeds: Process optimization using response surface methodology. in Journal of Supercritical Fluids
Elsevier Science Bv, Amsterdam., 107, 44-50.
https://doi.org/10.1016/j.supflu.2015.08.003

Bogdanović A, Tadić V, Stamenić M, Petrović S, Skala D. Supercritical carbon dioxide extraction of Trigonella foenum-graecum L. seeds: Process optimization using response surface methodology. in Journal of Supercritical Fluids. 2016;107:44-50.
doi:10.1016/j.supflu.2015.08.003 .

Bogdanović, Aleksandra, Tadić, Vanja, Stamenić, Marko, Petrović, Slobodan, Skala, Dejan, "Supercritical carbon dioxide extraction of Trigonella foenum-graecum L. seeds: Process optimization using response surface methodology" in Journal of Supercritical Fluids, 107 (2016):44-50,
https://doi.org/10.1016/j.supflu.2015.08.003 . .

TY  - JOUR
AU  - Milovanović, Stoja
AU  - Stamenić, Marko
AU  - Marković, Darka
AU  - Ivanović, Jasna
AU  - Žižović, Irena
PY  - 2015
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3043
AB  - Present work was aimed to investigate supercritical solvent impregnation of thymol into cellulose acetate in order to explore the possibility of developing a new functionalized solvent-free material with antimicrobial properties. Impregnation experiments were carried out with supercritical carbon dioxide at 10, 15 and 20 MPa, and at 35 and 50 degrees C. The time of impregnation was varied from 2 to 44h. Samples of impregnated cellulose acetate were tested against selected microorganisms. Thymol release kinetic studies were performed and obtained results were correlated. The impregnation yield varied from 4.51% to 72.26% depending on the operating conditions and impregnation time. Morphology of the samples changed significantly with the high impregnation yields, implying the impact of thymol on the cellulose acetate. The rate of impregnation was dependent on the impregnation conditions. Strong antimicrobial effect of impregnated samples against Escherichia colt, Staphylococcus aureus and Candida albicans strains with microbial reduction up to 99.9% was observed. Depending on the impregnation yield, samples of impregnated cellulose acetate have continuously released thymol in period from 2 to 21 days.
PB  - Elsevier Science Bv, Amsterdam
T2  - Journal of Supercritical Fluids
T1  - Supercritical impregnation of cellulose acetate with thymol
EP  - 115
SP  - 107
VL  - 97
DO  - 10.1016/j.supflu.2014.11.011
ER  - 

@article{
author = "Milovanović, Stoja and Stamenić, Marko and Marković, Darka and Ivanović, Jasna and Žižović, Irena",
year = "2015",
abstract = "Present work was aimed to investigate supercritical solvent impregnation of thymol into cellulose acetate in order to explore the possibility of developing a new functionalized solvent-free material with antimicrobial properties. Impregnation experiments were carried out with supercritical carbon dioxide at 10, 15 and 20 MPa, and at 35 and 50 degrees C. The time of impregnation was varied from 2 to 44h. Samples of impregnated cellulose acetate were tested against selected microorganisms. Thymol release kinetic studies were performed and obtained results were correlated. The impregnation yield varied from 4.51% to 72.26% depending on the operating conditions and impregnation time. Morphology of the samples changed significantly with the high impregnation yields, implying the impact of thymol on the cellulose acetate. The rate of impregnation was dependent on the impregnation conditions. Strong antimicrobial effect of impregnated samples against Escherichia colt, Staphylococcus aureus and Candida albicans strains with microbial reduction up to 99.9% was observed. Depending on the impregnation yield, samples of impregnated cellulose acetate have continuously released thymol in period from 2 to 21 days.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Journal of Supercritical Fluids",
title = "Supercritical impregnation of cellulose acetate with thymol",
pages = "115-107",
volume = "97",
doi = "10.1016/j.supflu.2014.11.011"
}

Milovanović, S., Stamenić, M., Marković, D., Ivanović, J.,& Žižović, I.. (2015). Supercritical impregnation of cellulose acetate with thymol. in Journal of Supercritical Fluids
Elsevier Science Bv, Amsterdam., 97, 107-115.
https://doi.org/10.1016/j.supflu.2014.11.011

Milovanović S, Stamenić M, Marković D, Ivanović J, Žižović I. Supercritical impregnation of cellulose acetate with thymol. in Journal of Supercritical Fluids. 2015;97:107-115.
doi:10.1016/j.supflu.2014.11.011 .

Milovanović, Stoja, Stamenić, Marko, Marković, Darka, Ivanović, Jasna, Žižović, Irena, "Supercritical impregnation of cellulose acetate with thymol" in Journal of Supercritical Fluids, 97 (2015):107-115,
https://doi.org/10.1016/j.supflu.2014.11.011 . .

TY  - CONF
AU  - Marković, Darka
AU  - Milovanović, Stoja
AU  - Stamenić, Marko
AU  - Jokić, Bojan
AU  - Žižović, Irena
AU  - Radetić, Maja
PY  - 2014
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6498
AB  - This study discusses the possibility of impregnation of corona pre-treated polypropylene (PP) non-woven fabric with thymol in supercritical carbon dioxide. Corona treatment of PP fibers caused significant morphological changes. FTIR measurements revealed that ester bond between OH group of thymol and carbonyl group of corona treated PP fibers has been established. Antibacterial activity of corona activated PP non-woven fabrics impregnated with thymol was tested against Gram-negative bacteria E. coli and Gram-positive bacteria S. aureus. Maximum bacteria reduction was provided.
PB  - Institute of Physics, Belgrade, Serbia
PB  - Klett izdavačka kuća doo, Belgrade, Serbia
C3  - 27th Summer School and International Symposium on the Physics of Ionized Gases, August 26-29, 2014, Belgrade, Serbia
T1  - The Impregnation of Corona Activated Polypropylene Non-woven Fabric with Thymol in Supercritical Carbon-dioxide
EP  - 422
SP  - 419
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6498
ER  - 

@conference{
author = "Marković, Darka and Milovanović, Stoja and Stamenić, Marko and Jokić, Bojan and Žižović, Irena and Radetić, Maja",
year = "2014",
abstract = "This study discusses the possibility of impregnation of corona pre-treated polypropylene (PP) non-woven fabric with thymol in supercritical carbon dioxide. Corona treatment of PP fibers caused significant morphological changes. FTIR measurements revealed that ester bond between OH group of thymol and carbonyl group of corona treated PP fibers has been established. Antibacterial activity of corona activated PP non-woven fabrics impregnated with thymol was tested against Gram-negative bacteria E. coli and Gram-positive bacteria S. aureus. Maximum bacteria reduction was provided.",
publisher = "Institute of Physics, Belgrade, Serbia, Klett izdavačka kuća doo, Belgrade, Serbia",
journal = "27th Summer School and International Symposium on the Physics of Ionized Gases, August 26-29, 2014, Belgrade, Serbia",
title = "The Impregnation of Corona Activated Polypropylene Non-woven Fabric with Thymol in Supercritical Carbon-dioxide",
pages = "422-419",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6498"
}

Marković, D., Milovanović, S., Stamenić, M., Jokić, B., Žižović, I.,& Radetić, M.. (2014). The Impregnation of Corona Activated Polypropylene Non-woven Fabric with Thymol in Supercritical Carbon-dioxide. in 27th Summer School and International Symposium on the Physics of Ionized Gases, August 26-29, 2014, Belgrade, Serbia
Institute of Physics, Belgrade, Serbia., 419-422.
https://hdl.handle.net/21.15107/rcub_technorep_6498

Marković D, Milovanović S, Stamenić M, Jokić B, Žižović I, Radetić M. The Impregnation of Corona Activated Polypropylene Non-woven Fabric with Thymol in Supercritical Carbon-dioxide. in 27th Summer School and International Symposium on the Physics of Ionized Gases, August 26-29, 2014, Belgrade, Serbia. 2014;:419-422.
https://hdl.handle.net/21.15107/rcub_technorep_6498 .

Marković, Darka, Milovanović, Stoja, Stamenić, Marko, Jokić, Bojan, Žižović, Irena, Radetić, Maja, "The Impregnation of Corona Activated Polypropylene Non-woven Fabric with Thymol in Supercritical Carbon-dioxide" in 27th Summer School and International Symposium on the Physics of Ionized Gases, August 26-29, 2014, Belgrade, Serbia (2014):419-422,
https://hdl.handle.net/21.15107/rcub_technorep_6498 .

TY  - CHAP
AU  - Ivanović, Jasna
AU  - Milovanović, Stoja
AU  - Stamenić, Marko
AU  - Fanovich, Marija Alejandra
AU  - Jaeger, Philip
AU  - Žižović, Irena
PY  - 2014
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2566
AB  - Supercritical carbon dioxide (sc2) is known as a good solvent for a wide range of bioactive compounds from natural sources. Extraction of bioactive compounds from plant material using sc2 is a favorable technique for producing solvent-free extracts suitable for a wide range of applications in pharmaceutical, biomedical, cosmetic and food industries. Incorporation of drugs or other bioactive substances into polymeric matrices by conventional techniques is connected to some significant drawbacks (use of organic solvents, undesired substances reactions and/or degradation, low incorporation yields and heterogeneous dispersion). Supercritical solvent impregnation (SSI), and namely impregnation using sc2, has been proposed as an alternative methodology to overcome most of these problems. The main advantages of this technique are avoidance of organic solvents and possibility to work at relatively low operational temperatures and withhydrophobic drugs/substances which cannot be impregnated by aqueous solution/suspension soaking methods. Additionally, unique physical and transport properties of sc2 can be used simultaneously for impregnation of biopolymers andtailoring the chemistry and morphology thereof to obtain the desired microstructure of the final product. The present chapter is particularly aimed to demonstrate feasibility of incorporating of thyme extracts into different solid matrices by using a methodology that combines the supercritical fluid extraction (SFE) and SSI. Compared to both processes to be conducted separately, the advantage of using one single integrated process is basically to save energy and time by avoiding an intermediate decompression procedure. Representative commercial carriers with relevance for pharmaceutical, biomedical and food applications (cotton gauze, polypropylene non-woven fabrics, chitosan and starch gels, cellulose acetate and polycaprolactone) were used to test feasibility of thyme extract incorporation by the coupled SFE-SSI process. By setting the relevant parameters of each process separately, the challenge of this methodology aims to harmonize these parameters in order to increase the extraction and impregnation efficiencies as well as to obtain an even distribution of the solute within the solid matrix.
T2  - Handbook on Supercritical Fluids: Fundamentals, Properties and Applications
T1  - Application of an integrated supercritical extraction and impregnation process for incorporation of thyme extracts into different carriers
EP  - 280
SP  - 258
UR  - https://hdl.handle.net/21.15107/rcub_technorep_2566
ER  - 

@inbook{
author = "Ivanović, Jasna and Milovanović, Stoja and Stamenić, Marko and Fanovich, Marija Alejandra and Jaeger, Philip and Žižović, Irena",
year = "2014",
abstract = "Supercritical carbon dioxide (sc2) is known as a good solvent for a wide range of bioactive compounds from natural sources. Extraction of bioactive compounds from plant material using sc2 is a favorable technique for producing solvent-free extracts suitable for a wide range of applications in pharmaceutical, biomedical, cosmetic and food industries. Incorporation of drugs or other bioactive substances into polymeric matrices by conventional techniques is connected to some significant drawbacks (use of organic solvents, undesired substances reactions and/or degradation, low incorporation yields and heterogeneous dispersion). Supercritical solvent impregnation (SSI), and namely impregnation using sc2, has been proposed as an alternative methodology to overcome most of these problems. The main advantages of this technique are avoidance of organic solvents and possibility to work at relatively low operational temperatures and withhydrophobic drugs/substances which cannot be impregnated by aqueous solution/suspension soaking methods. Additionally, unique physical and transport properties of sc2 can be used simultaneously for impregnation of biopolymers andtailoring the chemistry and morphology thereof to obtain the desired microstructure of the final product. The present chapter is particularly aimed to demonstrate feasibility of incorporating of thyme extracts into different solid matrices by using a methodology that combines the supercritical fluid extraction (SFE) and SSI. Compared to both processes to be conducted separately, the advantage of using one single integrated process is basically to save energy and time by avoiding an intermediate decompression procedure. Representative commercial carriers with relevance for pharmaceutical, biomedical and food applications (cotton gauze, polypropylene non-woven fabrics, chitosan and starch gels, cellulose acetate and polycaprolactone) were used to test feasibility of thyme extract incorporation by the coupled SFE-SSI process. By setting the relevant parameters of each process separately, the challenge of this methodology aims to harmonize these parameters in order to increase the extraction and impregnation efficiencies as well as to obtain an even distribution of the solute within the solid matrix.",
journal = "Handbook on Supercritical Fluids: Fundamentals, Properties and Applications",
booktitle = "Application of an integrated supercritical extraction and impregnation process for incorporation of thyme extracts into different carriers",
pages = "280-258",
url = "https://hdl.handle.net/21.15107/rcub_technorep_2566"
}

Ivanović, J., Milovanović, S., Stamenić, M., Fanovich, M. A., Jaeger, P.,& Žižović, I.. (2014). Application of an integrated supercritical extraction and impregnation process for incorporation of thyme extracts into different carriers. in Handbook on Supercritical Fluids: Fundamentals, Properties and Applications, 258-280.
https://hdl.handle.net/21.15107/rcub_technorep_2566

Ivanović J, Milovanović S, Stamenić M, Fanovich MA, Jaeger P, Žižović I. Application of an integrated supercritical extraction and impregnation process for incorporation of thyme extracts into different carriers. in Handbook on Supercritical Fluids: Fundamentals, Properties and Applications. 2014;:258-280.
https://hdl.handle.net/21.15107/rcub_technorep_2566 .

Ivanović, Jasna, Milovanović, Stoja, Stamenić, Marko, Fanovich, Marija Alejandra, Jaeger, Philip, Žižović, Irena, "Application of an integrated supercritical extraction and impregnation process for incorporation of thyme extracts into different carriers" in Handbook on Supercritical Fluids: Fundamentals, Properties and Applications (2014):258-280,
https://hdl.handle.net/21.15107/rcub_technorep_2566 .

TY  - JOUR
AU  - Ivanović, Jasna
AU  - Meyer, Florian
AU  - Stamenić, Marko
AU  - Jaeger, Philip
AU  - Žižović, Irena
AU  - Eggers, Rudolf
PY  - 2014
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2665
AB  - A proper method was to be developed for the pretreatment of different types of natural materials in order to increase the efficiency of supercritical fluid extraction (SFE) regarding yield and active substance content. The effects of different mechanical pretreatments were investigated: flaking, impact plus shearing, and cutting plus grinding. Additionally, the influence of intermediate rapid gas decompression was studied. Significant effects of the applied pretreatments on the yield (increase by up to 1350 %) were shown for SFE of selected oilseeds, artemisia aerial parts, and the lichen Usnea barbata. Flaking was the most effective pretreatment for increasing the yield of SFE of fennel seeds, artemisia aerial parts, and Usnea lichen, while impact plus shearing was optimal for processing amaranth, milk thistle, and oat seeds. The use of defined optimal pretreatments led to notable increases in the contents of usnic acid (50 %) and artemisinin (300 %).
PB  - Wiley-VCH Verlag Gmbh, Weinheim
T2  - Chemical Engineering & Technology
T1  - Pretreatment of Natural Materials Used for Supercritical Fluid Extraction of Commercial Phytopharmaceuticals
EP  - 1611
IS  - 9
SP  - 1606
VL  - 37
DO  - 10.1002/ceat.201300715
ER  - 

@article{
author = "Ivanović, Jasna and Meyer, Florian and Stamenić, Marko and Jaeger, Philip and Žižović, Irena and Eggers, Rudolf",
year = "2014",
abstract = "A proper method was to be developed for the pretreatment of different types of natural materials in order to increase the efficiency of supercritical fluid extraction (SFE) regarding yield and active substance content. The effects of different mechanical pretreatments were investigated: flaking, impact plus shearing, and cutting plus grinding. Additionally, the influence of intermediate rapid gas decompression was studied. Significant effects of the applied pretreatments on the yield (increase by up to 1350 %) were shown for SFE of selected oilseeds, artemisia aerial parts, and the lichen Usnea barbata. Flaking was the most effective pretreatment for increasing the yield of SFE of fennel seeds, artemisia aerial parts, and Usnea lichen, while impact plus shearing was optimal for processing amaranth, milk thistle, and oat seeds. The use of defined optimal pretreatments led to notable increases in the contents of usnic acid (50 %) and artemisinin (300 %).",
publisher = "Wiley-VCH Verlag Gmbh, Weinheim",
journal = "Chemical Engineering & Technology",
title = "Pretreatment of Natural Materials Used for Supercritical Fluid Extraction of Commercial Phytopharmaceuticals",
pages = "1611-1606",
number = "9",
volume = "37",
doi = "10.1002/ceat.201300715"
}

Ivanović, J., Meyer, F., Stamenić, M., Jaeger, P., Žižović, I.,& Eggers, R.. (2014). Pretreatment of Natural Materials Used for Supercritical Fluid Extraction of Commercial Phytopharmaceuticals. in Chemical Engineering & Technology
Wiley-VCH Verlag Gmbh, Weinheim., 37(9), 1606-1611.
https://doi.org/10.1002/ceat.201300715

Ivanović J, Meyer F, Stamenić M, Jaeger P, Žižović I, Eggers R. Pretreatment of Natural Materials Used for Supercritical Fluid Extraction of Commercial Phytopharmaceuticals. in Chemical Engineering & Technology. 2014;37(9):1606-1611.
doi:10.1002/ceat.201300715 .

Ivanović, Jasna, Meyer, Florian, Stamenić, Marko, Jaeger, Philip, Žižović, Irena, Eggers, Rudolf, "Pretreatment of Natural Materials Used for Supercritical Fluid Extraction of Commercial Phytopharmaceuticals" in Chemical Engineering & Technology, 37, no. 9 (2014):1606-1611,
https://doi.org/10.1002/ceat.201300715 . .

TY  - JOUR
AU  - Ivanović, Jasna
AU  - Tadić, Vanja
AU  - Dimitrijević, Suzana
AU  - Stamenić, Marko
AU  - Petrović, Slobodan
AU  - Žižović, Irena
PY  - 2014
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2675
AB  - For the first time, an ultrasound-assisted extraction was used to investigate isolation of anthocyanin-containing extracts from the blackberry cultivar "Cacanska Bestrna" widely grown in Serbia. Sonication time and temperature influence on the yield, active substances content and antioxidant activity of the extracts isolated from blackberry fruit pur e was discussed. Ultrasound-assisted extraction with acidified ethanol at room and moderately high temperature (40 degrees C) enabled rapid isolation (15-30 min) of anthocyanin-containing blackberry extracts with the high yields (5.3-6.3%). Both increase of the sonication temperature and time within studied ranges had positive effect on the extraction yield. The HPLC analysis revealed the presence of the anthocyanins in the investigated samples. After hydrolysis of all the samples, aglycone-cyanidin was detected in the samples. Determined cyanidin content in the obtained blackberry extracts (0.7-1.0%) was up to 20 times higher than in the blackberry juice. Cyanidin content was positively affected by sonication time and temperature increase. The antioxidant properties of the extracts were analyzed by the ferric reducing antioxidant power (FRAP) and 2,2-diphenyl-1-picryihydrazyl (DPPH) radical scavenging capacity assays. Positive effect of increase of the sonication time and/or temperature on the scavenging activity of the extracts against free DPPH radicals and thus lower IC50 values was demonstrated. Strong linear correlation of DPPH radical scavenging capacities of the extracts with content of cyanidin and tannins was established. FRAP values significantly correlated with total tannin content in the extracts. The analyses of composition and antioxidant properties in this study revealed great potential of blackberry ultrasonic extracts of the cultivar "Cacanska Bestrna" for further use in food and pharmaceutical industries.
PB  - Elsevier, Amsterdam
T2  - Industrial Crops and Products
T1  - Antioxidant properties of the anthocyanin-containing ultrasonic extract from blackberry cultivar "Cacanska Bestrna"
EP  - 281
SP  - 274
VL  - 53
DO  - 10.1016/j.indcrop.2013.12.048
ER  - 

@article{
author = "Ivanović, Jasna and Tadić, Vanja and Dimitrijević, Suzana and Stamenić, Marko and Petrović, Slobodan and Žižović, Irena",
year = "2014",
abstract = "For the first time, an ultrasound-assisted extraction was used to investigate isolation of anthocyanin-containing extracts from the blackberry cultivar "Cacanska Bestrna" widely grown in Serbia. Sonication time and temperature influence on the yield, active substances content and antioxidant activity of the extracts isolated from blackberry fruit pur e was discussed. Ultrasound-assisted extraction with acidified ethanol at room and moderately high temperature (40 degrees C) enabled rapid isolation (15-30 min) of anthocyanin-containing blackberry extracts with the high yields (5.3-6.3%). Both increase of the sonication temperature and time within studied ranges had positive effect on the extraction yield. The HPLC analysis revealed the presence of the anthocyanins in the investigated samples. After hydrolysis of all the samples, aglycone-cyanidin was detected in the samples. Determined cyanidin content in the obtained blackberry extracts (0.7-1.0%) was up to 20 times higher than in the blackberry juice. Cyanidin content was positively affected by sonication time and temperature increase. The antioxidant properties of the extracts were analyzed by the ferric reducing antioxidant power (FRAP) and 2,2-diphenyl-1-picryihydrazyl (DPPH) radical scavenging capacity assays. Positive effect of increase of the sonication time and/or temperature on the scavenging activity of the extracts against free DPPH radicals and thus lower IC50 values was demonstrated. Strong linear correlation of DPPH radical scavenging capacities of the extracts with content of cyanidin and tannins was established. FRAP values significantly correlated with total tannin content in the extracts. The analyses of composition and antioxidant properties in this study revealed great potential of blackberry ultrasonic extracts of the cultivar "Cacanska Bestrna" for further use in food and pharmaceutical industries.",
publisher = "Elsevier, Amsterdam",
journal = "Industrial Crops and Products",
title = "Antioxidant properties of the anthocyanin-containing ultrasonic extract from blackberry cultivar "Cacanska Bestrna"",
pages = "281-274",
volume = "53",
doi = "10.1016/j.indcrop.2013.12.048"
}

Ivanović, J., Tadić, V., Dimitrijević, S., Stamenić, M., Petrović, S.,& Žižović, I.. (2014). Antioxidant properties of the anthocyanin-containing ultrasonic extract from blackberry cultivar "Cacanska Bestrna". in Industrial Crops and Products
Elsevier, Amsterdam., 53, 274-281.
https://doi.org/10.1016/j.indcrop.2013.12.048

Ivanović J, Tadić V, Dimitrijević S, Stamenić M, Petrović S, Žižović I. Antioxidant properties of the anthocyanin-containing ultrasonic extract from blackberry cultivar "Cacanska Bestrna". in Industrial Crops and Products. 2014;53:274-281.
doi:10.1016/j.indcrop.2013.12.048 .

Ivanović, Jasna, Tadić, Vanja, Dimitrijević, Suzana, Stamenić, Marko, Petrović, Slobodan, Žižović, Irena, "Antioxidant properties of the anthocyanin-containing ultrasonic extract from blackberry cultivar "Cacanska Bestrna"" in Industrial Crops and Products, 53 (2014):274-281,
https://doi.org/10.1016/j.indcrop.2013.12.048 . .

TY  - JOUR
AU  - Stamenić, Marko
AU  - Ivanović, Jasna
AU  - Grujić, Slavica
AU  - Milovanović, Stoja
AU  - Žižović, Irena
AU  - Petrović, Slobodan
PY  - 2014
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2764
AB  - The present study was aimed to compare different mathematical models used to simulate process of supercritical extraction from commercially important Lamiaceae herbs. Supercritical extractions with carbon dioxide from rosemary, sage, thyme and lavender originated from the southern Balkan region were performed at 10MPa and 40 degrees C. Obtained extraction curves were simulated by the model based on the heat transfer analogy, Sovova's model and the model on the micro-scale. The models were used to analyse mass transfer phenomena and to quantify mass transfer parameters in the fluid and/or solid phase as well as the solubility of the solute in supercritical phase.
PB  - Wiley, Hoboken
T2  - Canadian Journal of Chemical Engineering
T1  - Comparative analysis of mathematical models for supercritical extraction simulation from industrially valuable lamiaceae herbs
EP  - 81
IS  - 1
SP  - 75
VL  - 92
DO  - 10.1002/cjce.21789
ER  - 

@article{
author = "Stamenić, Marko and Ivanović, Jasna and Grujić, Slavica and Milovanović, Stoja and Žižović, Irena and Petrović, Slobodan",
year = "2014",
abstract = "The present study was aimed to compare different mathematical models used to simulate process of supercritical extraction from commercially important Lamiaceae herbs. Supercritical extractions with carbon dioxide from rosemary, sage, thyme and lavender originated from the southern Balkan region were performed at 10MPa and 40 degrees C. Obtained extraction curves were simulated by the model based on the heat transfer analogy, Sovova's model and the model on the micro-scale. The models were used to analyse mass transfer phenomena and to quantify mass transfer parameters in the fluid and/or solid phase as well as the solubility of the solute in supercritical phase.",
publisher = "Wiley, Hoboken",
journal = "Canadian Journal of Chemical Engineering",
title = "Comparative analysis of mathematical models for supercritical extraction simulation from industrially valuable lamiaceae herbs",
pages = "81-75",
number = "1",
volume = "92",
doi = "10.1002/cjce.21789"
}

Stamenić, M., Ivanović, J., Grujić, S., Milovanović, S., Žižović, I.,& Petrović, S.. (2014). Comparative analysis of mathematical models for supercritical extraction simulation from industrially valuable lamiaceae herbs. in Canadian Journal of Chemical Engineering
Wiley, Hoboken., 92(1), 75-81.
https://doi.org/10.1002/cjce.21789

Stamenić M, Ivanović J, Grujić S, Milovanović S, Žižović I, Petrović S. Comparative analysis of mathematical models for supercritical extraction simulation from industrially valuable lamiaceae herbs. in Canadian Journal of Chemical Engineering. 2014;92(1):75-81.
doi:10.1002/cjce.21789 .

Stamenić, Marko, Ivanović, Jasna, Grujić, Slavica, Milovanović, Stoja, Žižović, Irena, Petrović, Slobodan, "Comparative analysis of mathematical models for supercritical extraction simulation from industrially valuable lamiaceae herbs" in Canadian Journal of Chemical Engineering, 92, no. 1 (2014):75-81,
https://doi.org/10.1002/cjce.21789 . .

TY  - CHAP
AU  - Meyer, Florian
AU  - Stamenić, Marko
AU  - Žižović, Irena
AU  - Eggers, Rudolf
PY  - 2014
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2568
AB  - A new trend in mathematical modeling of supercritical extraction process from plant material is presented.The most of the previously published models considered properties of the fixed bed of plant material during the extraction to be constant. This assumption might be quite true in the case of plant materials with relatively low quantity of extractables. However, the fixed bed property change may occur in the case of extraction from material with high quantities of extractables. Recently, mathematical model which took into account particle density, bed porosity and particle diameter as variables was derived. In this chapter, the model is further improved by introducing variable solubility of the extract in supercritical fluid. On the basis of new experimental results on the binary equilibrium of the system supercritical fluid - extract present in solid, a relationship between solubility in supercritical fluid and oil content in solid was established. The new model was derived which took into account particle density, bed porosity, particle diameter and solubility in supercritical fluid as variables. The model was verified on results from experiments in which the kintecis of supercritical extraction from rapseed was analyzed. Parameters of the model showed expected behaviour with respect to the change of particle size. Further analysis, shown in this chapter, showed that, if the dependency of solubility from the concentration in the solid phase is not accounted for, the model tends to overestimate the yield of the extraction.The new model is so far, the most realistic model and one of the most demanding models regarding experimental work and mathematical tools for the description of mass transfer in fixed beds.
T2  - Mathematical Modeling in Social Sciences and Engineering
T1  - Advances in Mathematical Modeling of Supercritical Extraction Processes
EP  - 70
SP  - 59
UR  - https://hdl.handle.net/21.15107/rcub_technorep_2568
ER  - 

@inbook{
author = "Meyer, Florian and Stamenić, Marko and Žižović, Irena and Eggers, Rudolf",
year = "2014",
abstract = "A new trend in mathematical modeling of supercritical extraction process from plant material is presented.The most of the previously published models considered properties of the fixed bed of plant material during the extraction to be constant. This assumption might be quite true in the case of plant materials with relatively low quantity of extractables. However, the fixed bed property change may occur in the case of extraction from material with high quantities of extractables. Recently, mathematical model which took into account particle density, bed porosity and particle diameter as variables was derived. In this chapter, the model is further improved by introducing variable solubility of the extract in supercritical fluid. On the basis of new experimental results on the binary equilibrium of the system supercritical fluid - extract present in solid, a relationship between solubility in supercritical fluid and oil content in solid was established. The new model was derived which took into account particle density, bed porosity, particle diameter and solubility in supercritical fluid as variables. The model was verified on results from experiments in which the kintecis of supercritical extraction from rapseed was analyzed. Parameters of the model showed expected behaviour with respect to the change of particle size. Further analysis, shown in this chapter, showed that, if the dependency of solubility from the concentration in the solid phase is not accounted for, the model tends to overestimate the yield of the extraction.The new model is so far, the most realistic model and one of the most demanding models regarding experimental work and mathematical tools for the description of mass transfer in fixed beds.",
journal = "Mathematical Modeling in Social Sciences and Engineering",
booktitle = "Advances in Mathematical Modeling of Supercritical Extraction Processes",
pages = "70-59",
url = "https://hdl.handle.net/21.15107/rcub_technorep_2568"
}

Meyer, F., Stamenić, M., Žižović, I.,& Eggers, R.. (2014). Advances in Mathematical Modeling of Supercritical Extraction Processes. in Mathematical Modeling in Social Sciences and Engineering, 59-70.
https://hdl.handle.net/21.15107/rcub_technorep_2568

Meyer F, Stamenić M, Žižović I, Eggers R. Advances in Mathematical Modeling of Supercritical Extraction Processes. in Mathematical Modeling in Social Sciences and Engineering. 2014;:59-70.
https://hdl.handle.net/21.15107/rcub_technorep_2568 .

Meyer, Florian, Stamenić, Marko, Žižović, Irena, Eggers, Rudolf, "Advances in Mathematical Modeling of Supercritical Extraction Processes" in Mathematical Modeling in Social Sciences and Engineering (2014):59-70,
https://hdl.handle.net/21.15107/rcub_technorep_2568 .

TY  - JOUR
AU  - Stamenić, Marko
AU  - Vulić, Jelena
AU  - Đilas, Sonja
AU  - Mišić, Dušan
AU  - Tadić, Vanja
AU  - Petrović, Slobodan
AU  - Žižović, Irena
PY  - 2014
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2655
AB  - The antioxidant and antibacterial properties of Greek oregano extracts obtained by fractional supercritical fluid extraction (SFE) with carbon dioxide were investigated and compared with the properties of essential oil obtained by hydrodistillation. According to DPPH., hydroxyl radical and superoxide anion radical scavenging activity assays, the supercritical extracts expressed stronger antioxidant activity comparing to the essential oil. The most effective was the supercritical extract obtained by fractional extraction at 30 MPa and 100 degrees C after the volatile fraction had been extracted at lower pressure. At the same time this extract showed strong antibacterial activity against staphylococci, including MASA strain, but did not affect Escherichia coli of normal intestinal flora. The essential oil obtained by hydrodistillation showed stronger antibacterial activity against E. coli, Salmonella and Klebsiella pneumoniae, comparing to the supercritical extracts but at the same affected the normal gut flora.
PB  - Elsevier Sci Ltd, Oxford
T2  - Food Chemistry
T1  - Free-radical scavenging activity and antibacterial impact of Greek oregano isolates obtained by SFE
EP  - 315
SP  - 307
VL  - 165
DO  - 10.1016/j.foodchem.2014.05.091
ER  - 

@article{
author = "Stamenić, Marko and Vulić, Jelena and Đilas, Sonja and Mišić, Dušan and Tadić, Vanja and Petrović, Slobodan and Žižović, Irena",
year = "2014",
abstract = "The antioxidant and antibacterial properties of Greek oregano extracts obtained by fractional supercritical fluid extraction (SFE) with carbon dioxide were investigated and compared with the properties of essential oil obtained by hydrodistillation. According to DPPH., hydroxyl radical and superoxide anion radical scavenging activity assays, the supercritical extracts expressed stronger antioxidant activity comparing to the essential oil. The most effective was the supercritical extract obtained by fractional extraction at 30 MPa and 100 degrees C after the volatile fraction had been extracted at lower pressure. At the same time this extract showed strong antibacterial activity against staphylococci, including MASA strain, but did not affect Escherichia coli of normal intestinal flora. The essential oil obtained by hydrodistillation showed stronger antibacterial activity against E. coli, Salmonella and Klebsiella pneumoniae, comparing to the supercritical extracts but at the same affected the normal gut flora.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Food Chemistry",
title = "Free-radical scavenging activity and antibacterial impact of Greek oregano isolates obtained by SFE",
pages = "315-307",
volume = "165",
doi = "10.1016/j.foodchem.2014.05.091"
}

Stamenić, M., Vulić, J., Đilas, S., Mišić, D., Tadić, V., Petrović, S.,& Žižović, I.. (2014). Free-radical scavenging activity and antibacterial impact of Greek oregano isolates obtained by SFE. in Food Chemistry
Elsevier Sci Ltd, Oxford., 165, 307-315.
https://doi.org/10.1016/j.foodchem.2014.05.091

Stamenić M, Vulić J, Đilas S, Mišić D, Tadić V, Petrović S, Žižović I. Free-radical scavenging activity and antibacterial impact of Greek oregano isolates obtained by SFE. in Food Chemistry. 2014;165:307-315.
doi:10.1016/j.foodchem.2014.05.091 .

Stamenić, Marko, Vulić, Jelena, Đilas, Sonja, Mišić, Dušan, Tadić, Vanja, Petrović, Slobodan, Žižović, Irena, "Free-radical scavenging activity and antibacterial impact of Greek oregano isolates obtained by SFE" in Food Chemistry, 165 (2014):307-315,
https://doi.org/10.1016/j.foodchem.2014.05.091 . .

TY  - CONF
AU  - Milovanović, Stoja
AU  - Stamenić, Marko
AU  - Marković, Darka
AU  - Radetić, Maja
AU  - Žižović, Irena
PY  - 2013
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6503
PB  - Association of Chemical Engineers of Serbia, Belgrade
C3  - Proc. 6th International Symposium on High Pressure Processes Technology, 8-11 September, 2013, Belgrade, Serbia
T1  - Solubility of Thymol in Supercritical Carbon Dioxide and its Impregnation on Cotton Gauze
EP  - 173
SP  - 169
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6503
ER  - 

@conference{
author = "Milovanović, Stoja and Stamenić, Marko and Marković, Darka and Radetić, Maja and Žižović, Irena",
year = "2013",
publisher = "Association of Chemical Engineers of Serbia, Belgrade",
journal = "Proc. 6th International Symposium on High Pressure Processes Technology, 8-11 September, 2013, Belgrade, Serbia",
title = "Solubility of Thymol in Supercritical Carbon Dioxide and its Impregnation on Cotton Gauze",
pages = "173-169",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6503"
}

Milovanović, S., Stamenić, M., Marković, D., Radetić, M.,& Žižović, I.. (2013). Solubility of Thymol in Supercritical Carbon Dioxide and its Impregnation on Cotton Gauze. in Proc. 6th International Symposium on High Pressure Processes Technology, 8-11 September, 2013, Belgrade, Serbia
Association of Chemical Engineers of Serbia, Belgrade., 169-173.
https://hdl.handle.net/21.15107/rcub_technorep_6503

Milovanović S, Stamenić M, Marković D, Radetić M, Žižović I. Solubility of Thymol in Supercritical Carbon Dioxide and its Impregnation on Cotton Gauze. in Proc. 6th International Symposium on High Pressure Processes Technology, 8-11 September, 2013, Belgrade, Serbia. 2013;:169-173.
https://hdl.handle.net/21.15107/rcub_technorep_6503 .

Milovanović, Stoja, Stamenić, Marko, Marković, Darka, Radetić, Maja, Žižović, Irena, "Solubility of Thymol in Supercritical Carbon Dioxide and its Impregnation on Cotton Gauze" in Proc. 6th International Symposium on High Pressure Processes Technology, 8-11 September, 2013, Belgrade, Serbia (2013):169-173,
https://hdl.handle.net/21.15107/rcub_technorep_6503 .

TY  - JOUR
AU  - Milovanović, Stoja
AU  - Stamenić, Marko
AU  - Marković, Darka
AU  - Radetić, Maja
AU  - Žižović, Irena
PY  - 2013
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2497
AB  - Through this study, an attempt has been made to evaluate the solubility of thymol in supercritical carbon dioxide as well to investigate a prospect of its impregnation on cotton gauze on laboratory scale. Solubility of thymol in supercritical carbon dioxide was determined at temperatures of 35 degrees C, 40 degrees C and 50 degrees C, and pressures ranging from 7.8 to 25 MPa (CO2 density range 335.89-849.60 kg/m(3)) using a static method. The solubility data were correlated using semi-empirical equations introduced by Chrastil, Adachi and Lu and del Valle and Aguilera. Taking into account obtained results, temperature of 35 degrees C and pressure of 15.5 MPa were selected as operating conditions for the impregnation process. Impregnation of cotton gauze with thymol was performed in a cell using carbon dioxide as a solvent. Kinetics of the process was determined and modeled. Masses of thymol on cotton gauzes after 2 h and 24 h of impregnation were 11% and 19.6%, respectively. FT-IR analysis confirmed the presence of thymol on the surface of the cotton fibers. The impregnated gauze provided strong antimicrobial activity against tested strains of Escherichia coli, Staphylococcus aureus, Bacillus subtilis, Enterococcus faecalis and Candida albicans.
PB  - Elsevier Science Bv, Amsterdam
T2  - Journal of Supercritical Fluids
T1  - Solubility of thymol in supercritical carbon dioxide and its impregnation on cotton gauze
EP  - 181
SP  - 173
VL  - 84
DO  - 10.1016/j.supflu.2013.10.003
ER  - 

@article{
author = "Milovanović, Stoja and Stamenić, Marko and Marković, Darka and Radetić, Maja and Žižović, Irena",
year = "2013",
abstract = "Through this study, an attempt has been made to evaluate the solubility of thymol in supercritical carbon dioxide as well to investigate a prospect of its impregnation on cotton gauze on laboratory scale. Solubility of thymol in supercritical carbon dioxide was determined at temperatures of 35 degrees C, 40 degrees C and 50 degrees C, and pressures ranging from 7.8 to 25 MPa (CO2 density range 335.89-849.60 kg/m(3)) using a static method. The solubility data were correlated using semi-empirical equations introduced by Chrastil, Adachi and Lu and del Valle and Aguilera. Taking into account obtained results, temperature of 35 degrees C and pressure of 15.5 MPa were selected as operating conditions for the impregnation process. Impregnation of cotton gauze with thymol was performed in a cell using carbon dioxide as a solvent. Kinetics of the process was determined and modeled. Masses of thymol on cotton gauzes after 2 h and 24 h of impregnation were 11% and 19.6%, respectively. FT-IR analysis confirmed the presence of thymol on the surface of the cotton fibers. The impregnated gauze provided strong antimicrobial activity against tested strains of Escherichia coli, Staphylococcus aureus, Bacillus subtilis, Enterococcus faecalis and Candida albicans.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Journal of Supercritical Fluids",
title = "Solubility of thymol in supercritical carbon dioxide and its impregnation on cotton gauze",
pages = "181-173",
volume = "84",
doi = "10.1016/j.supflu.2013.10.003"
}

Milovanović, S., Stamenić, M., Marković, D., Radetić, M.,& Žižović, I.. (2013). Solubility of thymol in supercritical carbon dioxide and its impregnation on cotton gauze. in Journal of Supercritical Fluids
Elsevier Science Bv, Amsterdam., 84, 173-181.
https://doi.org/10.1016/j.supflu.2013.10.003

Milovanović S, Stamenić M, Marković D, Radetić M, Žižović I. Solubility of thymol in supercritical carbon dioxide and its impregnation on cotton gauze. in Journal of Supercritical Fluids. 2013;84:173-181.
doi:10.1016/j.supflu.2013.10.003 .

Milovanović, Stoja, Stamenić, Marko, Marković, Darka, Radetić, Maja, Žižović, Irena, "Solubility of thymol in supercritical carbon dioxide and its impregnation on cotton gauze" in Journal of Supercritical Fluids, 84 (2013):173-181,
https://doi.org/10.1016/j.supflu.2013.10.003 . .

TY  - JOUR
AU  - Stamenić, Marko
AU  - Žižović, Irena
PY  - 2013
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2485
AB  - This article deals with mathematical tools used for solving equations of the improved mathematical model on the micro-scale for the process of supercritical fluid extraction of essential oils from glandular trichomes. Glandular trichomes are secretory structures of Lamiaceae plant family and as such represent the sites of essential oil synthesis and storage. It was previously noticed that during the extraction with carbon dioxide these secretory structures undergo cracking due to the solvent dissolving into the essential oil phase. In this study, the process of extraction is thoroughly analysed and mathematically presented on the fixed bed scale as well as on the single trichome scale. The finite differences method was applied for solving differential equations of the model. This included dividing the extractor vessel into twenty spatial, and extraction time into ten thousand time increments. Cracking time distribution of glandular trichomes in the form of Gamma distribution was incorporated in each of the twenty spatial increments. The model was applied to simulate experimental results of supercritical extraction from several species of the Lamiaceae family. The deviation of the model results from the experimental data was 9.6-35.7% lower for the improved model than for the model without the cracking time distribution function.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Computers & Chemical Engineering
T1  - The mathematics of modelling the supercritical fluid extraction of essential oils from glandular trichomes
EP  - 95
SP  - 89
VL  - 48
DO  - 10.1016/j.compchemeng.2012.08.006
ER  - 

@article{
author = "Stamenić, Marko and Žižović, Irena",
year = "2013",
abstract = "This article deals with mathematical tools used for solving equations of the improved mathematical model on the micro-scale for the process of supercritical fluid extraction of essential oils from glandular trichomes. Glandular trichomes are secretory structures of Lamiaceae plant family and as such represent the sites of essential oil synthesis and storage. It was previously noticed that during the extraction with carbon dioxide these secretory structures undergo cracking due to the solvent dissolving into the essential oil phase. In this study, the process of extraction is thoroughly analysed and mathematically presented on the fixed bed scale as well as on the single trichome scale. The finite differences method was applied for solving differential equations of the model. This included dividing the extractor vessel into twenty spatial, and extraction time into ten thousand time increments. Cracking time distribution of glandular trichomes in the form of Gamma distribution was incorporated in each of the twenty spatial increments. The model was applied to simulate experimental results of supercritical extraction from several species of the Lamiaceae family. The deviation of the model results from the experimental data was 9.6-35.7% lower for the improved model than for the model without the cracking time distribution function.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Computers & Chemical Engineering",
title = "The mathematics of modelling the supercritical fluid extraction of essential oils from glandular trichomes",
pages = "95-89",
volume = "48",
doi = "10.1016/j.compchemeng.2012.08.006"
}

Stamenić, M.,& Žižović, I.. (2013). The mathematics of modelling the supercritical fluid extraction of essential oils from glandular trichomes. in Computers & Chemical Engineering
Pergamon-Elsevier Science Ltd, Oxford., 48, 89-95.
https://doi.org/10.1016/j.compchemeng.2012.08.006

Stamenić M, Žižović I. The mathematics of modelling the supercritical fluid extraction of essential oils from glandular trichomes. in Computers & Chemical Engineering. 2013;48:89-95.
doi:10.1016/j.compchemeng.2012.08.006 .

Stamenić, Marko, Žižović, Irena, "The mathematics of modelling the supercritical fluid extraction of essential oils from glandular trichomes" in Computers & Chemical Engineering, 48 (2013):89-95,
https://doi.org/10.1016/j.compchemeng.2012.08.006 . .

TY  - JOUR
AU  - Žižović, Irena
AU  - Ivanović, Jasna
AU  - Mišić, Dušan
AU  - Stamenić, Marko
AU  - Đorđević, Sofija
AU  - Kukić-Marković, Jelena
AU  - Petrović, Slobodan
PY  - 2012
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2123
AB  - The number of cases of fatal infections in humans and animals caused by multiresistant and panresistant bacterial strains has been dramatically increased over the past 10 years on almost every continent. Therefore, implementation of newer technologies in order to develop processes for isolation of substances with strong antibacterial activities from natural sources is of increasing interest. In this study, advantage of high pressure processing and supercritical fluid extraction with carbon dioxide in isolation of active principles from lichen Usnea barbata L was presented. Supercritical extraction showed extremely higher selectivity toward usnic acid compared to the conventional techniques tested. In order to simulate the process and estimate mass transfer coefficients, the new mathematical model of Sovova (2011) was applied. Supercritical extract obtained at 40 C and 30 MPa showed stronger antibacterial activity against the most of tested strains compared to pure usnic acid and extracts obtained using ethanol as a solvent. Further, supercritical extract with lower usnic acid content expressed stronger antibacterial activity against the majority of tested strains including MRSA strains compared to the pure usnic acid and the supercritical extract with the higher usnic acid content. The same extract was far more effective than ampicillin against Streptococcus uberis, all tested Enterococcus faecalis, MRSA and MRSA ATCC 33591. Against few tested strains, the supercritical extract was superior to erythromycin and penicillin as well. These findings justified possible benefits of inclusion of supercritical extracts into pharmaceutical and cosmetic formulations. Applied mathematical model described the SFE process with high accuracy.
PB  - Elsevier Science Bv, Amsterdam
T2  - Journal of Supercritical Fluids
T1  - SFE as a superior technique for isolation of extracts with strong antibacterial activities from lichen Usnea barbata L.
EP  - 14
SP  - 7
VL  - 72
DO  - 10.1016/j.supflu.2012.07.018
ER  - 

@article{
author = "Žižović, Irena and Ivanović, Jasna and Mišić, Dušan and Stamenić, Marko and Đorđević, Sofija and Kukić-Marković, Jelena and Petrović, Slobodan",
year = "2012",
abstract = "The number of cases of fatal infections in humans and animals caused by multiresistant and panresistant bacterial strains has been dramatically increased over the past 10 years on almost every continent. Therefore, implementation of newer technologies in order to develop processes for isolation of substances with strong antibacterial activities from natural sources is of increasing interest. In this study, advantage of high pressure processing and supercritical fluid extraction with carbon dioxide in isolation of active principles from lichen Usnea barbata L was presented. Supercritical extraction showed extremely higher selectivity toward usnic acid compared to the conventional techniques tested. In order to simulate the process and estimate mass transfer coefficients, the new mathematical model of Sovova (2011) was applied. Supercritical extract obtained at 40 C and 30 MPa showed stronger antibacterial activity against the most of tested strains compared to pure usnic acid and extracts obtained using ethanol as a solvent. Further, supercritical extract with lower usnic acid content expressed stronger antibacterial activity against the majority of tested strains including MRSA strains compared to the pure usnic acid and the supercritical extract with the higher usnic acid content. The same extract was far more effective than ampicillin against Streptococcus uberis, all tested Enterococcus faecalis, MRSA and MRSA ATCC 33591. Against few tested strains, the supercritical extract was superior to erythromycin and penicillin as well. These findings justified possible benefits of inclusion of supercritical extracts into pharmaceutical and cosmetic formulations. Applied mathematical model described the SFE process with high accuracy.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Journal of Supercritical Fluids",
title = "SFE as a superior technique for isolation of extracts with strong antibacterial activities from lichen Usnea barbata L.",
pages = "14-7",
volume = "72",
doi = "10.1016/j.supflu.2012.07.018"
}

Žižović, I., Ivanović, J., Mišić, D., Stamenić, M., Đorđević, S., Kukić-Marković, J.,& Petrović, S.. (2012). SFE as a superior technique for isolation of extracts with strong antibacterial activities from lichen Usnea barbata L.. in Journal of Supercritical Fluids
Elsevier Science Bv, Amsterdam., 72, 7-14.
https://doi.org/10.1016/j.supflu.2012.07.018

Žižović I, Ivanović J, Mišić D, Stamenić M, Đorđević S, Kukić-Marković J, Petrović S. SFE as a superior technique for isolation of extracts with strong antibacterial activities from lichen Usnea barbata L.. in Journal of Supercritical Fluids. 2012;72:7-14.
doi:10.1016/j.supflu.2012.07.018 .

Žižović, Irena, Ivanović, Jasna, Mišić, Dušan, Stamenić, Marko, Đorđević, Sofija, Kukić-Marković, Jelena, Petrović, Slobodan, "SFE as a superior technique for isolation of extracts with strong antibacterial activities from lichen Usnea barbata L." in Journal of Supercritical Fluids, 72 (2012):7-14,
https://doi.org/10.1016/j.supflu.2012.07.018 . .

TY  - JOUR
AU  - Meyer, Florian
AU  - Stamenić, Marko
AU  - Žižović, Irena
AU  - Eggers, Rudolf
PY  - 2012
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2167
AB  - Properties of the fixed bed of plant material during the extraction with scCO(2) from rapeseed were investigated for the two types of mechanical pretreatments: flaking and impact milling. Obtained results indicated that particle density, bulk density of the bed and consequently the bed porosity changed during the extraction. In the case of flaked rapeseed, the change of particle diameter was also detected and quantified. On the basis of experimental data linear relationships between the particle density/bed porosity/mean particle diameter and oil content in solid were established. Mathematical model based on the mass balances for the supercritical and solid phase which took into account particle density, bed porosity and particle diameter (for flaked plant material) as variables was derived. The proposed model described experimental data with high accuracy. Possible error due to neglecting the change of the fixed bed properties was presented as well.
PB  - Elsevier Science Bv, Amsterdam
T2  - Journal of Supercritical Fluids
T1  - Fixed bed property changes during scCO(2) extraction of natural materials - Experiments and modeling
EP  - 149
SP  - 140
VL  - 72
DO  - 10.1016/j.supflu.2012.08.022
ER  - 

@article{
author = "Meyer, Florian and Stamenić, Marko and Žižović, Irena and Eggers, Rudolf",
year = "2012",
abstract = "Properties of the fixed bed of plant material during the extraction with scCO(2) from rapeseed were investigated for the two types of mechanical pretreatments: flaking and impact milling. Obtained results indicated that particle density, bulk density of the bed and consequently the bed porosity changed during the extraction. In the case of flaked rapeseed, the change of particle diameter was also detected and quantified. On the basis of experimental data linear relationships between the particle density/bed porosity/mean particle diameter and oil content in solid were established. Mathematical model based on the mass balances for the supercritical and solid phase which took into account particle density, bed porosity and particle diameter (for flaked plant material) as variables was derived. The proposed model described experimental data with high accuracy. Possible error due to neglecting the change of the fixed bed properties was presented as well.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Journal of Supercritical Fluids",
title = "Fixed bed property changes during scCO(2) extraction of natural materials - Experiments and modeling",
pages = "149-140",
volume = "72",
doi = "10.1016/j.supflu.2012.08.022"
}

Meyer, F., Stamenić, M., Žižović, I.,& Eggers, R.. (2012). Fixed bed property changes during scCO(2) extraction of natural materials - Experiments and modeling. in Journal of Supercritical Fluids
Elsevier Science Bv, Amsterdam., 72, 140-149.
https://doi.org/10.1016/j.supflu.2012.08.022

Meyer F, Stamenić M, Žižović I, Eggers R. Fixed bed property changes during scCO(2) extraction of natural materials - Experiments and modeling. in Journal of Supercritical Fluids. 2012;72:140-149.
doi:10.1016/j.supflu.2012.08.022 .

Meyer, Florian, Stamenić, Marko, Žižović, Irena, Eggers, Rudolf, "Fixed bed property changes during scCO(2) extraction of natural materials - Experiments and modeling" in Journal of Supercritical Fluids, 72 (2012):140-149,
https://doi.org/10.1016/j.supflu.2012.08.022 . .