TY  - JOUR
AU  - Vanheuverzwijn, Jérome
AU  - Maillard, Eloise-Eliane
AU  - Mahat, Amal
AU  - Fowler, Lee
AU  - Monteyne, Daniel
AU  - Bonnaud, Leïla
AU  - Landercy, Nicolas
AU  - Hemberg, Axel
AU  - Janković, Ana
AU  - Meyer, Franck
AU  - Mišković-Stanković, Vesna
AU  - Stevanović, Milena
AU  - Mirica, Codruta
AU  - Pérez-Morga, David
AU  - Luginbuehl, Reto
AU  - Combes, Christèle
AU  - Furtos, Gabriel
AU  - Fontaine, Véronique
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6381
AB  - Medical implants have improved the quality of life of many patients. However, surgical intervention may eventually lead to implant microbial contamination. The aims of this research were to develop an easy, robust, quantitative assay to assess surface antimicrobial activities, especially the anti-nascent biofilm activity, and to identify control surfaces, allowing for international comparisons. Using new antimicrobial assays to assess the inhibition of nascent biofilm during persistent contact or after transient contact with bacteria, we show that the 5 cent Euro coin or other metal-based antibacterial coins can be used as positive controls, as more than 4 log reduction on bacterial survival was observed when using either S. aureus or P. aeruginosa as targets. The methods and controls described here could be useful to develop an easy, flexible and standardizable assay to assess relevant antimicrobial activities of new implant materials developed by industries and academics.
PB  - MDPI
T2  - Microorganisms
T1  - Easy, Flexible and Standardizable Anti-Nascent Biofilm Activity Assay to Assess Implant Materials
IS  - 4
SP  - 1023
VL  - 11
DO  - 10.3390/microorganisms11041023
ER  - 

@article{
author = "Vanheuverzwijn, Jérome and Maillard, Eloise-Eliane and Mahat, Amal and Fowler, Lee and Monteyne, Daniel and Bonnaud, Leïla and Landercy, Nicolas and Hemberg, Axel and Janković, Ana and Meyer, Franck and Mišković-Stanković, Vesna and Stevanović, Milena and Mirica, Codruta and Pérez-Morga, David and Luginbuehl, Reto and Combes, Christèle and Furtos, Gabriel and Fontaine, Véronique",
year = "2023",
abstract = "Medical implants have improved the quality of life of many patients. However, surgical intervention may eventually lead to implant microbial contamination. The aims of this research were to develop an easy, robust, quantitative assay to assess surface antimicrobial activities, especially the anti-nascent biofilm activity, and to identify control surfaces, allowing for international comparisons. Using new antimicrobial assays to assess the inhibition of nascent biofilm during persistent contact or after transient contact with bacteria, we show that the 5 cent Euro coin or other metal-based antibacterial coins can be used as positive controls, as more than 4 log reduction on bacterial survival was observed when using either S. aureus or P. aeruginosa as targets. The methods and controls described here could be useful to develop an easy, flexible and standardizable assay to assess relevant antimicrobial activities of new implant materials developed by industries and academics.",
publisher = "MDPI",
journal = "Microorganisms",
title = "Easy, Flexible and Standardizable Anti-Nascent Biofilm Activity Assay to Assess Implant Materials",
number = "4",
pages = "1023",
volume = "11",
doi = "10.3390/microorganisms11041023"
}

Vanheuverzwijn, J., Maillard, E., Mahat, A., Fowler, L., Monteyne, D., Bonnaud, L., Landercy, N., Hemberg, A., Janković, A., Meyer, F., Mišković-Stanković, V., Stevanović, M., Mirica, C., Pérez-Morga, D., Luginbuehl, R., Combes, C., Furtos, G.,& Fontaine, V.. (2023). Easy, Flexible and Standardizable Anti-Nascent Biofilm Activity Assay to Assess Implant Materials. in Microorganisms
MDPI., 11(4), 1023.
https://doi.org/10.3390/microorganisms11041023

Vanheuverzwijn J, Maillard E, Mahat A, Fowler L, Monteyne D, Bonnaud L, Landercy N, Hemberg A, Janković A, Meyer F, Mišković-Stanković V, Stevanović M, Mirica C, Pérez-Morga D, Luginbuehl R, Combes C, Furtos G, Fontaine V. Easy, Flexible and Standardizable Anti-Nascent Biofilm Activity Assay to Assess Implant Materials. in Microorganisms. 2023;11(4):1023.
doi:10.3390/microorganisms11041023 .

Vanheuverzwijn, Jérome, Maillard, Eloise-Eliane, Mahat, Amal, Fowler, Lee, Monteyne, Daniel, Bonnaud, Leïla, Landercy, Nicolas, Hemberg, Axel, Janković, Ana, Meyer, Franck, Mišković-Stanković, Vesna, Stevanović, Milena, Mirica, Codruta, Pérez-Morga, David, Luginbuehl, Reto, Combes, Christèle, Furtos, Gabriel, Fontaine, Véronique, "Easy, Flexible and Standardizable Anti-Nascent Biofilm Activity Assay to Assess Implant Materials" in Microorganisms, 11, no. 4 (2023):1023,
https://doi.org/10.3390/microorganisms11041023 . .