TY  - CONF
AU  - Stevanović, Milena
AU  - Đošić, Marija
AU  - Janković, Ana
AU  - Sprecher, Christoph Martin
AU  - Vukašinović-Sekulić, Maja
AU  - Mišković-Stanković, Vesna
PY  - 2024
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7442
AB  - Tissue engineering strives for innovative solutions in addressing challenges associated with contemporary bone tissue implants. This study focuses on the electrophoretic deposition (EPD) of hydroxyapatite-based bioceramic composites containing antibacterial agents onto titanium surfaces. Two composite coatings, hydroxyapa-tite/chitosan (HAP/CS) and hydroxyapatite/chitosan/gentamicin (HAP/CS/Gent) were developed to combat issues such as poor adhesion, limited antibacterial potential, limited bioactivity, and potential toxicity of implant materials [1].
PB  - Savez hemijskih inženjera Srbije
C3  - Hemijska industrija - Supplementary Issue - ExcellMater Conference 2024 Abstracts
T1  - Innovative hydroxyapatite-based coatings for bone implants: A multifaceted approach
IS  - 1S
SP  - 58
VL  - 78
UR  - https://hdl.handle.net/21.15107/rcub_technorep_7442
ER  - 

@conference{
author = "Stevanović, Milena and Đošić, Marija and Janković, Ana and Sprecher, Christoph Martin and Vukašinović-Sekulić, Maja and Mišković-Stanković, Vesna",
year = "2024",
abstract = "Tissue engineering strives for innovative solutions in addressing challenges associated with contemporary bone tissue implants. This study focuses on the electrophoretic deposition (EPD) of hydroxyapatite-based bioceramic composites containing antibacterial agents onto titanium surfaces. Two composite coatings, hydroxyapa-tite/chitosan (HAP/CS) and hydroxyapatite/chitosan/gentamicin (HAP/CS/Gent) were developed to combat issues such as poor adhesion, limited antibacterial potential, limited bioactivity, and potential toxicity of implant materials [1].",
publisher = "Savez hemijskih inženjera Srbije",
journal = "Hemijska industrija - Supplementary Issue - ExcellMater Conference 2024 Abstracts",
title = "Innovative hydroxyapatite-based coatings for bone implants: A multifaceted approach",
number = "1S",
pages = "58",
volume = "78",
url = "https://hdl.handle.net/21.15107/rcub_technorep_7442"
}

Stevanović, M., Đošić, M., Janković, A., Sprecher, C. M., Vukašinović-Sekulić, M.,& Mišković-Stanković, V.. (2024). Innovative hydroxyapatite-based coatings for bone implants: A multifaceted approach. in Hemijska industrija - Supplementary Issue - ExcellMater Conference 2024 Abstracts
Savez hemijskih inženjera Srbije., 78(1S), 58.
https://hdl.handle.net/21.15107/rcub_technorep_7442

Stevanović M, Đošić M, Janković A, Sprecher CM, Vukašinović-Sekulić M, Mišković-Stanković V. Innovative hydroxyapatite-based coatings for bone implants: A multifaceted approach. in Hemijska industrija - Supplementary Issue - ExcellMater Conference 2024 Abstracts. 2024;78(1S):58.
https://hdl.handle.net/21.15107/rcub_technorep_7442 .

Stevanović, Milena, Đošić, Marija, Janković, Ana, Sprecher, Christoph Martin, Vukašinović-Sekulić, Maja, Mišković-Stanković, Vesna, "Innovative hydroxyapatite-based coatings for bone implants: A multifaceted approach" in Hemijska industrija - Supplementary Issue - ExcellMater Conference 2024 Abstracts, 78, no. 1S (2024):58,
https://hdl.handle.net/21.15107/rcub_technorep_7442 .

TY  - CONF
AU  - Vukašinović Sekulić, Maja
AU  - Rakin, Marica
AU  - Bulatović, Maja
AU  - Krunić, Tanja
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7187
AB  - The aim of this study is to compare the antimicrobial activity of clove
essential oil against probiotic strains isolated from different pharmaceutical
preparations currently available on the local market (Lacticaseibacillus rhamnosus GG,
Lactiplantibacillus plantarum 299v, Lactobacillus acidophilus, Saccharomyces
boulardii).
PB  - Leskovac : Faculty of Technology
C3  - Book of abstracts / 15th International Symposium "Novel Technologies and Sustainable Development" Leskovac, October, 20-21, 2023
T1  - Comparison of the antimicrobial activity of clove essential oil against probiotic microorganisms
SP  - 51
UR  - https://hdl.handle.net/21.15107/rcub_technorep_7187
ER  - 

@conference{
author = "Vukašinović Sekulić, Maja and Rakin, Marica and Bulatović, Maja and Krunić, Tanja",
year = "2023",
abstract = "The aim of this study is to compare the antimicrobial activity of clove
essential oil against probiotic strains isolated from different pharmaceutical
preparations currently available on the local market (Lacticaseibacillus rhamnosus GG,
Lactiplantibacillus plantarum 299v, Lactobacillus acidophilus, Saccharomyces
boulardii).",
publisher = "Leskovac : Faculty of Technology",
journal = "Book of abstracts / 15th International Symposium "Novel Technologies and Sustainable Development" Leskovac, October, 20-21, 2023",
title = "Comparison of the antimicrobial activity of clove essential oil against probiotic microorganisms",
pages = "51",
url = "https://hdl.handle.net/21.15107/rcub_technorep_7187"
}

Vukašinović Sekulić, M., Rakin, M., Bulatović, M.,& Krunić, T.. (2023). Comparison of the antimicrobial activity of clove essential oil against probiotic microorganisms. in Book of abstracts / 15th International Symposium "Novel Technologies and Sustainable Development" Leskovac, October, 20-21, 2023
Leskovac : Faculty of Technology., 51.
https://hdl.handle.net/21.15107/rcub_technorep_7187

Vukašinović Sekulić M, Rakin M, Bulatović M, Krunić T. Comparison of the antimicrobial activity of clove essential oil against probiotic microorganisms. in Book of abstracts / 15th International Symposium "Novel Technologies and Sustainable Development" Leskovac, October, 20-21, 2023. 2023;:51.
https://hdl.handle.net/21.15107/rcub_technorep_7187 .

Vukašinović Sekulić, Maja, Rakin, Marica, Bulatović, Maja, Krunić, Tanja, "Comparison of the antimicrobial activity of clove essential oil against probiotic microorganisms" in Book of abstracts / 15th International Symposium "Novel Technologies and Sustainable Development" Leskovac, October, 20-21, 2023 (2023):51,
https://hdl.handle.net/21.15107/rcub_technorep_7187 .

TY  - CONF
AU  - Rakin, Marica
AU  - Bulatović, Maja
AU  - Živanović, Sara
AU  - Milutinović, Milica
AU  - Rakin, Duška
AU  - Zarić, Danica
AU  - Vukašinović-Sekulić, Maja
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7013
AB  - The search for waste minimization possibilities and the valorization of by-products are key to
good management and improved sustainability in the food industry. Although dairy products
still remain at the forefront of probiotic food development, in recent years non-dairy food
products have increased in popularity owing to their unique characteristics and advantages.
Due to its beneficial properties, caused by the presence of various bioactive compounds, the
almond parts that remain after almond milk production can be considered promising sources
of ingredients for the development of non-dairy food products.
The aim of this study was to examine the potential and possibility of using a surplus product
that remains after almond milk production. The antioxidant properties of the remained surplus
product were characterized based on the polyphenol, flavonoid, and anthocyanin content as
well as FRAP, DPPH, and ABTS antioxidant activity. The fermented beverage that combines
the properties of almonds and probiotic bacteria, formulated using inulin, lyophilized fruit and
aroma, was evaluated for physicochemical, microbiological, and sensory properties during cold
storage (4 °C, 21 day).
The surplus product that remains after the almond milk production contains 61.4 mg GAE/100g
DW, 16.11 mg QE/100g DW, and 0.993 mg CYE/100g DW of polyphenols, flavonoids, and
anthocyanins (respectively), and expresses DPPH, FRAP and ABTS antioxidant activity of
71.56 mg DW/mL, 50.47 mg DW/mL and 53.11 mg DW/mL (respectively).
The fermentation of surplus product that remains after almond milk production leads to the
production of the beverage with satisfactory values of quality parameters as follows: pH value
of 4,75, titratable acidity 28,8 °SH, syneresis 7,5%, viable cell count of 7.77 log (CFU mL-1),
and sensory characteristics value of 10, that is stable during 21 day of cold storage.
A new probiotic almond-based beverage that combines the properties of both almonds and
probiotics can be considered as promising step toward the circular bioeconomy.
PB  - Novi Sad : Faculty of Technology
C3  - Proceedings of the 2nd International Conference on Advanced Production and Processing ICAPP 2022, Novi Sad
T1  - Probiotic almond-based beverage: promising step towards a circular bioeconomy
EP  - 64
SP  - 54
UR  - https://hdl.handle.net/21.15107/rcub_technorep_7013
ER  - 

@conference{
author = "Rakin, Marica and Bulatović, Maja and Živanović, Sara and Milutinović, Milica and Rakin, Duška and Zarić, Danica and Vukašinović-Sekulić, Maja",
year = "2023",
abstract = "The search for waste minimization possibilities and the valorization of by-products are key to
good management and improved sustainability in the food industry. Although dairy products
still remain at the forefront of probiotic food development, in recent years non-dairy food
products have increased in popularity owing to their unique characteristics and advantages.
Due to its beneficial properties, caused by the presence of various bioactive compounds, the
almond parts that remain after almond milk production can be considered promising sources
of ingredients for the development of non-dairy food products.
The aim of this study was to examine the potential and possibility of using a surplus product
that remains after almond milk production. The antioxidant properties of the remained surplus
product were characterized based on the polyphenol, flavonoid, and anthocyanin content as
well as FRAP, DPPH, and ABTS antioxidant activity. The fermented beverage that combines
the properties of almonds and probiotic bacteria, formulated using inulin, lyophilized fruit and
aroma, was evaluated for physicochemical, microbiological, and sensory properties during cold
storage (4 °C, 21 day).
The surplus product that remains after the almond milk production contains 61.4 mg GAE/100g
DW, 16.11 mg QE/100g DW, and 0.993 mg CYE/100g DW of polyphenols, flavonoids, and
anthocyanins (respectively), and expresses DPPH, FRAP and ABTS antioxidant activity of
71.56 mg DW/mL, 50.47 mg DW/mL and 53.11 mg DW/mL (respectively).
The fermentation of surplus product that remains after almond milk production leads to the
production of the beverage with satisfactory values of quality parameters as follows: pH value
of 4,75, titratable acidity 28,8 °SH, syneresis 7,5%, viable cell count of 7.77 log (CFU mL-1),
and sensory characteristics value of 10, that is stable during 21 day of cold storage.
A new probiotic almond-based beverage that combines the properties of both almonds and
probiotics can be considered as promising step toward the circular bioeconomy.",
publisher = "Novi Sad : Faculty of Technology",
journal = "Proceedings of the 2nd International Conference on Advanced Production and Processing ICAPP 2022, Novi Sad",
title = "Probiotic almond-based beverage: promising step towards a circular bioeconomy",
pages = "64-54",
url = "https://hdl.handle.net/21.15107/rcub_technorep_7013"
}

Rakin, M., Bulatović, M., Živanović, S., Milutinović, M., Rakin, D., Zarić, D.,& Vukašinović-Sekulić, M.. (2023). Probiotic almond-based beverage: promising step towards a circular bioeconomy. in Proceedings of the 2nd International Conference on Advanced Production and Processing ICAPP 2022, Novi Sad
Novi Sad : Faculty of Technology., 54-64.
https://hdl.handle.net/21.15107/rcub_technorep_7013

Rakin M, Bulatović M, Živanović S, Milutinović M, Rakin D, Zarić D, Vukašinović-Sekulić M. Probiotic almond-based beverage: promising step towards a circular bioeconomy. in Proceedings of the 2nd International Conference on Advanced Production and Processing ICAPP 2022, Novi Sad. 2023;:54-64.
https://hdl.handle.net/21.15107/rcub_technorep_7013 .

Rakin, Marica, Bulatović, Maja, Živanović, Sara, Milutinović, Milica, Rakin, Duška, Zarić, Danica, Vukašinović-Sekulić, Maja, "Probiotic almond-based beverage: promising step towards a circular bioeconomy" in Proceedings of the 2nd International Conference on Advanced Production and Processing ICAPP 2022, Novi Sad (2023):54-64,
https://hdl.handle.net/21.15107/rcub_technorep_7013 .

TY  - JOUR
AU  - Đošić, Marija
AU  - Janković, Ana
AU  - Stevanović, Milena
AU  - Stojanović, Jovica
AU  - Vukašinović-Sekulić, Maja
AU  - Kojić, Vesna
AU  - Mišković-Stanković, Vesna
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6364
AB  - Electrophoretic deposition (EPD) was employed to fabricate composite coatings aimed for orthopedic implants. Hydroxyapatite (HAP), poly(vinyl alcohol) (PVA) and chitosan (CS) coatings, with or without antibiotic gentamicin (Gent) were deposited in a single-step process from aqueous suspension, using EPD. Polymer/mineral composite coatings were formed with the aim to mimic the natural composition of bone tissue, along with improved coatings antibacterial properties, at the same time serving as a drug carrier, allowing local administration of antibiotic. Structural and morphological characterization of HAP/PVA/CS and HAP/PVA/CS/Gent coatings was investigated by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), thermogravimetric analysis (TG/DTG), and scanning electron microscopy (SEM) proving carbonate-substituted HAP. The presence of gentamicin contributed to the bactericidal activity of composite HAP/PVA/CS/Gent coating against Staphylococcus aureus and Escherichia coli. Cytotoxicity studies were performed towards human MRC-5 and mice L929 fibroblast cell lines, and both investigated samples (HAP/PVA/CS and HAP/PVA/CS/Gent) expressed good biocompatibility, without causing toxic effect on tested cells. Biomineralization potential was investigated by measuring the alkaline phosphatase activity, demonstrating high potential of HAP/PVA/CS/Gent to induce the growth of new HAP layer. Therefore, the present investigation proposes the production of composite coatings from non-toxic (aqueous) suspensions, consisting of biocompatible and bioactive components, along with antibiotic gentamicin, improving the antibacterial coatings' properties.
PB  - Elsevier Ltd.
T2  - Materials Chemistry and Physics
T1  - Hydroxyapatite/poly(vinyl alcohol)/chitosan coating with gentamicin for orthopedic implants
SP  - 127766
VL  - 303
DO  - 10.1016/j.matchemphys.2023.127766
ER  - 

@article{
author = "Đošić, Marija and Janković, Ana and Stevanović, Milena and Stojanović, Jovica and Vukašinović-Sekulić, Maja and Kojić, Vesna and Mišković-Stanković, Vesna",
year = "2023",
abstract = "Electrophoretic deposition (EPD) was employed to fabricate composite coatings aimed for orthopedic implants. Hydroxyapatite (HAP), poly(vinyl alcohol) (PVA) and chitosan (CS) coatings, with or without antibiotic gentamicin (Gent) were deposited in a single-step process from aqueous suspension, using EPD. Polymer/mineral composite coatings were formed with the aim to mimic the natural composition of bone tissue, along with improved coatings antibacterial properties, at the same time serving as a drug carrier, allowing local administration of antibiotic. Structural and morphological characterization of HAP/PVA/CS and HAP/PVA/CS/Gent coatings was investigated by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), thermogravimetric analysis (TG/DTG), and scanning electron microscopy (SEM) proving carbonate-substituted HAP. The presence of gentamicin contributed to the bactericidal activity of composite HAP/PVA/CS/Gent coating against Staphylococcus aureus and Escherichia coli. Cytotoxicity studies were performed towards human MRC-5 and mice L929 fibroblast cell lines, and both investigated samples (HAP/PVA/CS and HAP/PVA/CS/Gent) expressed good biocompatibility, without causing toxic effect on tested cells. Biomineralization potential was investigated by measuring the alkaline phosphatase activity, demonstrating high potential of HAP/PVA/CS/Gent to induce the growth of new HAP layer. Therefore, the present investigation proposes the production of composite coatings from non-toxic (aqueous) suspensions, consisting of biocompatible and bioactive components, along with antibiotic gentamicin, improving the antibacterial coatings' properties.",
publisher = "Elsevier Ltd.",
journal = "Materials Chemistry and Physics",
title = "Hydroxyapatite/poly(vinyl alcohol)/chitosan coating with gentamicin for orthopedic implants",
pages = "127766",
volume = "303",
doi = "10.1016/j.matchemphys.2023.127766"
}

Đošić, M., Janković, A., Stevanović, M., Stojanović, J., Vukašinović-Sekulić, M., Kojić, V.,& Mišković-Stanković, V.. (2023). Hydroxyapatite/poly(vinyl alcohol)/chitosan coating with gentamicin for orthopedic implants. in Materials Chemistry and Physics
Elsevier Ltd.., 303, 127766.
https://doi.org/10.1016/j.matchemphys.2023.127766

Đošić M, Janković A, Stevanović M, Stojanović J, Vukašinović-Sekulić M, Kojić V, Mišković-Stanković V. Hydroxyapatite/poly(vinyl alcohol)/chitosan coating with gentamicin for orthopedic implants. in Materials Chemistry and Physics. 2023;303:127766.
doi:10.1016/j.matchemphys.2023.127766 .

Đošić, Marija, Janković, Ana, Stevanović, Milena, Stojanović, Jovica, Vukašinović-Sekulić, Maja, Kojić, Vesna, Mišković-Stanković, Vesna, "Hydroxyapatite/poly(vinyl alcohol)/chitosan coating with gentamicin for orthopedic implants" in Materials Chemistry and Physics, 303 (2023):127766,
https://doi.org/10.1016/j.matchemphys.2023.127766 . .

TY  - JOUR
AU  - Jaćimović, Nevena
AU  - Đošić, Marija
AU  - Janković, Ana
AU  - Grujić, Svetlana
AU  - Matić Bujagić, Ivana
AU  - Stojanović, Jovica
AU  - Vukašinović-Sekulić, Maja
AU  - Kojić, Vesna
AU  - Mišković-Stanković, Vesna
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7260
AB  - Biocomposite hydroxyapatite/poly(vinyl alcohol)/chitosan/gentamicin coatings were fabricated on titanium by an electrophoretic deposition process (EPD) from an aqueous suspension using the constant voltage method. Characterization of the deposited coatings was performed by scanning electron micros-copy with field emission (FE-SEM), Fourier transform infrared spectroscopy (FT-IR), and X-ray diffraction (XRD) after immersion in simulated body fluid (SBF) at 37 °C. The concentration of the released gentamicin was determined using high-performance liquid chromatography (HPLC) coupled with a mass spectrometer (MS). The release profile confirmed an initial “burst-release effect” with ~ 30 % of the gen-tamicin released in the first 48 h, which could be of assistance in the prevention of biofilm formation. An-tibacterial activity was proven by the agar diffusion method on Staphylococcus aureus TL and Escherich-ia coli ATCC 25922 bacterial strains. Cytotoxicity was tested by the dye exclusion test (DET) on MRC-5 and L929 fibroblast cell lines, with both coatings expressing nontoxicity. The results showed the high ap-plicability potential of a poly(vinyl alcohol)-based biocomposite coating for medical purposes.
PB  - Society of Chemists and Technologists of Macedonia
T2  - Macedonian Journal of Chemistry and Chemical Engineering
T1  - Single-step, electrophoretically deposited hydroxyapatite/poly(vinyl alcohol)/chitosan/gentamicin coating for biomedical applications
EP  - 262
IS  - 2
SP  - 249
VL  - 42
DO  - 10.20450/mjcce.2023.2775
ER  - 

@article{
author = "Jaćimović, Nevena and Đošić, Marija and Janković, Ana and Grujić, Svetlana and Matić Bujagić, Ivana and Stojanović, Jovica and Vukašinović-Sekulić, Maja and Kojić, Vesna and Mišković-Stanković, Vesna",
year = "2023",
abstract = "Biocomposite hydroxyapatite/poly(vinyl alcohol)/chitosan/gentamicin coatings were fabricated on titanium by an electrophoretic deposition process (EPD) from an aqueous suspension using the constant voltage method. Characterization of the deposited coatings was performed by scanning electron micros-copy with field emission (FE-SEM), Fourier transform infrared spectroscopy (FT-IR), and X-ray diffraction (XRD) after immersion in simulated body fluid (SBF) at 37 °C. The concentration of the released gentamicin was determined using high-performance liquid chromatography (HPLC) coupled with a mass spectrometer (MS). The release profile confirmed an initial “burst-release effect” with ~ 30 % of the gen-tamicin released in the first 48 h, which could be of assistance in the prevention of biofilm formation. An-tibacterial activity was proven by the agar diffusion method on Staphylococcus aureus TL and Escherich-ia coli ATCC 25922 bacterial strains. Cytotoxicity was tested by the dye exclusion test (DET) on MRC-5 and L929 fibroblast cell lines, with both coatings expressing nontoxicity. The results showed the high ap-plicability potential of a poly(vinyl alcohol)-based biocomposite coating for medical purposes.",
publisher = "Society of Chemists and Technologists of Macedonia",
journal = "Macedonian Journal of Chemistry and Chemical Engineering",
title = "Single-step, electrophoretically deposited hydroxyapatite/poly(vinyl alcohol)/chitosan/gentamicin coating for biomedical applications",
pages = "262-249",
number = "2",
volume = "42",
doi = "10.20450/mjcce.2023.2775"
}

Jaćimović, N., Đošić, M., Janković, A., Grujić, S., Matić Bujagić, I., Stojanović, J., Vukašinović-Sekulić, M., Kojić, V.,& Mišković-Stanković, V.. (2023). Single-step, electrophoretically deposited hydroxyapatite/poly(vinyl alcohol)/chitosan/gentamicin coating for biomedical applications. in Macedonian Journal of Chemistry and Chemical Engineering
Society of Chemists and Technologists of Macedonia., 42(2), 249-262.
https://doi.org/10.20450/mjcce.2023.2775

Jaćimović N, Đošić M, Janković A, Grujić S, Matić Bujagić I, Stojanović J, Vukašinović-Sekulić M, Kojić V, Mišković-Stanković V. Single-step, electrophoretically deposited hydroxyapatite/poly(vinyl alcohol)/chitosan/gentamicin coating for biomedical applications. in Macedonian Journal of Chemistry and Chemical Engineering. 2023;42(2):249-262.
doi:10.20450/mjcce.2023.2775 .

Jaćimović, Nevena, Đošić, Marija, Janković, Ana, Grujić, Svetlana, Matić Bujagić, Ivana, Stojanović, Jovica, Vukašinović-Sekulić, Maja, Kojić, Vesna, Mišković-Stanković, Vesna, "Single-step, electrophoretically deposited hydroxyapatite/poly(vinyl alcohol)/chitosan/gentamicin coating for biomedical applications" in Macedonian Journal of Chemistry and Chemical Engineering, 42, no. 2 (2023):249-262,
https://doi.org/10.20450/mjcce.2023.2775 . .

TY  - JOUR
AU  - Vukašinović, Mila
AU  - Pantelić, Ivana
AU  - Savić, Sanela
AU  - Cekić, Nebojša
AU  - Vukašinović Sekulić, Maja
AU  - Antić Stanković, Jelena
AU  - Božić, Dragana D.
AU  - Tošić, Anđela
AU  - Tamburić, Slobodanka
AU  - Savić, Snežana D.
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7071
AB  - Bioactive peptides are promising cosmetic active ingredients that can improve skin healthand appearance. They exhibit a broad spectrum of activity, including anti-aging, antioxidant, an-timicrobial, and anti-inflammatory effects. The aim of this study was to develop a safe, stable, andefficacious environmentally friendly (“green”) emulsion using a milk protein hydrolysate as a modelactive ingredient. Potential emulsions were formulated with biodegradable emollients, stabilizedwith naturally derived mixed emulsifier, and prepared by cold process. They were evaluated forrheological behavior (continuous rotation and oscillation tests), physical stability (dynamic me-chanical thermal analysis—DMTA test), and texture profiles, as well as cytotoxic, antioxidant, andantimicrobial effects. Rheological characterization revealed shear-thinning flow behavior with yieldpoint from continuous rotation tests and predominantly elastic character from oscillation (amplitudeand frequency sweep) tests, with small structural change detected in the DMTA test. These resultsimplied satisfactory rheological properties and good stability. Texture analysis revealed acceptablespreadability and substantivity of the emulsions. The protein hydrolysate showed antioxidant activity.The developed emulsions showed low antibacterial activity against selected microorganisms, butthis was due to the action of preservatives, not peptides. All potential emulsions showed a desirablesafety profile. The results obtained provide the basis for the next stage of formulation development,i.e., in vivo efficacy tests.
PB  - MDPI
T2  - Cosmetics
T1  - Development of a “Green” Emulsion with a Milk Protein Hydrolysate: An Evaluation of Rheology, Texture, In Vitro Bioactivity, and Safety
IS  - 6
SP  - 162
VL  - 10
DO  - 10.3390/cosmetics10060162
ER  - 

@article{
author = "Vukašinović, Mila and Pantelić, Ivana and Savić, Sanela and Cekić, Nebojša and Vukašinović Sekulić, Maja and Antić Stanković, Jelena and Božić, Dragana D. and Tošić, Anđela and Tamburić, Slobodanka and Savić, Snežana D.",
year = "2023",
abstract = "Bioactive peptides are promising cosmetic active ingredients that can improve skin healthand appearance. They exhibit a broad spectrum of activity, including anti-aging, antioxidant, an-timicrobial, and anti-inflammatory effects. The aim of this study was to develop a safe, stable, andefficacious environmentally friendly (“green”) emulsion using a milk protein hydrolysate as a modelactive ingredient. Potential emulsions were formulated with biodegradable emollients, stabilizedwith naturally derived mixed emulsifier, and prepared by cold process. They were evaluated forrheological behavior (continuous rotation and oscillation tests), physical stability (dynamic me-chanical thermal analysis—DMTA test), and texture profiles, as well as cytotoxic, antioxidant, andantimicrobial effects. Rheological characterization revealed shear-thinning flow behavior with yieldpoint from continuous rotation tests and predominantly elastic character from oscillation (amplitudeand frequency sweep) tests, with small structural change detected in the DMTA test. These resultsimplied satisfactory rheological properties and good stability. Texture analysis revealed acceptablespreadability and substantivity of the emulsions. The protein hydrolysate showed antioxidant activity.The developed emulsions showed low antibacterial activity against selected microorganisms, butthis was due to the action of preservatives, not peptides. All potential emulsions showed a desirablesafety profile. The results obtained provide the basis for the next stage of formulation development,i.e., in vivo efficacy tests.",
publisher = "MDPI",
journal = "Cosmetics",
title = "Development of a “Green” Emulsion with a Milk Protein Hydrolysate: An Evaluation of Rheology, Texture, In Vitro Bioactivity, and Safety",
number = "6",
pages = "162",
volume = "10",
doi = "10.3390/cosmetics10060162"
}

Vukašinović, M., Pantelić, I., Savić, S., Cekić, N., Vukašinović Sekulić, M., Antić Stanković, J., Božić, D. D., Tošić, A., Tamburić, S.,& Savić, S. D.. (2023). Development of a “Green” Emulsion with a Milk Protein Hydrolysate: An Evaluation of Rheology, Texture, In Vitro Bioactivity, and Safety. in Cosmetics
MDPI., 10(6), 162.
https://doi.org/10.3390/cosmetics10060162

Vukašinović M, Pantelić I, Savić S, Cekić N, Vukašinović Sekulić M, Antić Stanković J, Božić DD, Tošić A, Tamburić S, Savić SD. Development of a “Green” Emulsion with a Milk Protein Hydrolysate: An Evaluation of Rheology, Texture, In Vitro Bioactivity, and Safety. in Cosmetics. 2023;10(6):162.
doi:10.3390/cosmetics10060162 .

Vukašinović, Mila, Pantelić, Ivana, Savić, Sanela, Cekić, Nebojša, Vukašinović Sekulić, Maja, Antić Stanković, Jelena, Božić, Dragana D., Tošić, Anđela, Tamburić, Slobodanka, Savić, Snežana D., "Development of a “Green” Emulsion with a Milk Protein Hydrolysate: An Evaluation of Rheology, Texture, In Vitro Bioactivity, and Safety" in Cosmetics, 10, no. 6 (2023):162,
https://doi.org/10.3390/cosmetics10060162 . .

TY  - JOUR
AU  - Vidović, Srđan
AU  - Stojkovska, Jasmina
AU  - Stevanović, Milan
AU  - Balanč, Bojana
AU  - Vukašinović-Sekulić, Maja
AU  - Marinković, Aleksandar
AU  - Obradović, Bojana
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5105
AB  - In this work, nanocomposite fibres and microfibres based on alginate and poly(vinyl alcohol) (PVA) with silver nanoparticles (AgNPs) were produced and characterized for potential application as antibacterial wound dressings. PVA/Ag/Na-alginate colloid solution was used for the preparation of the fibres by a simple extrusion technique followed by freezing-thawing cycles. UV-Visible spectroscopy confirmed successful preservation of AgNPs in fibres while Fourier transform infrared spectroscopy has shown a balanced combined effect on the Ca-alginate spatial arrangement with the addition of both AgNPs and PVA. The presence of PVA in fibres induced an increase in the swelling degree as compared with that of Ag/Ca-alginate fibres (approx. 28 versus approx. 14). Still, the initially produced PVA/Ca-alginate fibres were mechanically weaker than Ca-alginate fibres, but after drying and rehydration exhibited better mechanical properties. Also, the obtained fibres released AgNPs and/or silver ions at the concentration of approximately 2.6 μg cm -3 leading to bacteriostatic effects against Staphylococcus aureus and Escherichia coli. These results are relevant for practical utilization of the fibres, which could be stored and applied in the dry form with preserved mechanical stability, sorption capacity and antibacterial activity.
PB  - Royal Society Publishing
T2  - Royal Society Open Science
T1  - Effects of poly(vinyl alcohol) blending with Ag/alginate solutions to form nanocomposite fibres for potential use as antibacterial wound dressings
IS  - 3
SP  - 211517
VL  - 9
DO  - 10.1098/rsos.211517
ER  - 

@article{
author = "Vidović, Srđan and Stojkovska, Jasmina and Stevanović, Milan and Balanč, Bojana and Vukašinović-Sekulić, Maja and Marinković, Aleksandar and Obradović, Bojana",
year = "2022",
abstract = "In this work, nanocomposite fibres and microfibres based on alginate and poly(vinyl alcohol) (PVA) with silver nanoparticles (AgNPs) were produced and characterized for potential application as antibacterial wound dressings. PVA/Ag/Na-alginate colloid solution was used for the preparation of the fibres by a simple extrusion technique followed by freezing-thawing cycles. UV-Visible spectroscopy confirmed successful preservation of AgNPs in fibres while Fourier transform infrared spectroscopy has shown a balanced combined effect on the Ca-alginate spatial arrangement with the addition of both AgNPs and PVA. The presence of PVA in fibres induced an increase in the swelling degree as compared with that of Ag/Ca-alginate fibres (approx. 28 versus approx. 14). Still, the initially produced PVA/Ca-alginate fibres were mechanically weaker than Ca-alginate fibres, but after drying and rehydration exhibited better mechanical properties. Also, the obtained fibres released AgNPs and/or silver ions at the concentration of approximately 2.6 μg cm -3 leading to bacteriostatic effects against Staphylococcus aureus and Escherichia coli. These results are relevant for practical utilization of the fibres, which could be stored and applied in the dry form with preserved mechanical stability, sorption capacity and antibacterial activity.",
publisher = "Royal Society Publishing",
journal = "Royal Society Open Science",
title = "Effects of poly(vinyl alcohol) blending with Ag/alginate solutions to form nanocomposite fibres for potential use as antibacterial wound dressings",
number = "3",
pages = "211517",
volume = "9",
doi = "10.1098/rsos.211517"
}

Vidović, S., Stojkovska, J., Stevanović, M., Balanč, B., Vukašinović-Sekulić, M., Marinković, A.,& Obradović, B.. (2022). Effects of poly(vinyl alcohol) blending with Ag/alginate solutions to form nanocomposite fibres for potential use as antibacterial wound dressings. in Royal Society Open Science
Royal Society Publishing., 9(3), 211517.
https://doi.org/10.1098/rsos.211517

Vidović S, Stojkovska J, Stevanović M, Balanč B, Vukašinović-Sekulić M, Marinković A, Obradović B. Effects of poly(vinyl alcohol) blending with Ag/alginate solutions to form nanocomposite fibres for potential use as antibacterial wound dressings. in Royal Society Open Science. 2022;9(3):211517.
doi:10.1098/rsos.211517 .

Vidović, Srđan, Stojkovska, Jasmina, Stevanović, Milan, Balanč, Bojana, Vukašinović-Sekulić, Maja, Marinković, Aleksandar, Obradović, Bojana, "Effects of poly(vinyl alcohol) blending with Ag/alginate solutions to form nanocomposite fibres for potential use as antibacterial wound dressings" in Royal Society Open Science, 9, no. 3 (2022):211517,
https://doi.org/10.1098/rsos.211517 . .

TY  - CONF
AU  - Nešović, Katarina
AU  - Janković, Ana
AU  - Surudžić, Rade
AU  - Vukašinović-Sekulić, Maja
AU  - Kojić, Vesna
AU  - Radetić, Tamara
AU  - Mišković-Stanković, Vesna
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6761
AB  - Recent advances in biomaterials science are increasingly focused
on (re)search for next-generation soft tissue regeneration solutions.
Hydrogel-based materials are becoming a focal point to address
many tissue engineering requirements, possessing exceptional biocompatibility,
with tailorable shape, structure and mechanical
properties, as well as infinite possibilities drug delivery. The widespread
antibiotics use has led to aggravated resistance issues in many
bacterial strains, so the focus is increasingly shifted towards alternative
antibacterial solutions such as silver nanoparticles (AgNPs).
In this work, we have developed novel composite materials based on
poly(vinyl alcohol)/chitosan (PVA/CHI) and poly(vinyl alcohol)/
chitosan/graphene (PVA/CHI/Gr) hydrogels with silver nanoparticles
obtained through green in situ constant-voltage electrochemical
synthesis. The obtained AgNPs’ properties, sizes and size
distributions were examined using UV-visible spectroscopy, dynamic
light scattering and transmission electron microscopy. In vitro
swelling and silver release kinetics were monitored in the simulated
physiological conditions (pH7.4, 37  C). The obtained release and
swelling isotherms were fitted with several theoretical models that
helped discern the mechanisms of these processes. Tensile testing
was carried out to examine the composites’ mechanical behavior and
elasticity – important properties for soft tissue engineering materials.
Finally, the excellent antibacterial activity of the obtained silver/
poly(vinyl alcohol)/chitosan (Ag/PVA/CHI) and silver/poly(vinyl
alcohol)/chitosan/graphene (Ag/PVA/CHI/Gr) hydrogels against
Staphylococcus aureus and Escherichia coli was confirmed quantitatively
by colony counting method, and their non-toxicity was
verified towards two model fibroblast cell lines (MRC-5 and L929)
utilizing the MTT assay. Through thorough characterization, the
obtained nanocomposite hydrogel materials were identified as strong
candidates for active antibacterial soft tissue engineering materials.
PB  - Mary Ann Liebert, Inc.
C3  - Tissue Engineering Part A
T1  - Silver Nanoparticles-Loaded Poly(Vinyl Alcohol)/Chitosan/Graphene Hydrogels Obtained by Electrochemical Synthesis
IS  - S1
SP  - S-132
VL  - 28
DO  - 10.1089/ten.tea.2022.29025.abstracts
ER  - 

@conference{
author = "Nešović, Katarina and Janković, Ana and Surudžić, Rade and Vukašinović-Sekulić, Maja and Kojić, Vesna and Radetić, Tamara and Mišković-Stanković, Vesna",
year = "2022",
abstract = "Recent advances in biomaterials science are increasingly focused
on (re)search for next-generation soft tissue regeneration solutions.
Hydrogel-based materials are becoming a focal point to address
many tissue engineering requirements, possessing exceptional biocompatibility,
with tailorable shape, structure and mechanical
properties, as well as infinite possibilities drug delivery. The widespread
antibiotics use has led to aggravated resistance issues in many
bacterial strains, so the focus is increasingly shifted towards alternative
antibacterial solutions such as silver nanoparticles (AgNPs).
In this work, we have developed novel composite materials based on
poly(vinyl alcohol)/chitosan (PVA/CHI) and poly(vinyl alcohol)/
chitosan/graphene (PVA/CHI/Gr) hydrogels with silver nanoparticles
obtained through green in situ constant-voltage electrochemical
synthesis. The obtained AgNPs’ properties, sizes and size
distributions were examined using UV-visible spectroscopy, dynamic
light scattering and transmission electron microscopy. In vitro
swelling and silver release kinetics were monitored in the simulated
physiological conditions (pH7.4, 37  C). The obtained release and
swelling isotherms were fitted with several theoretical models that
helped discern the mechanisms of these processes. Tensile testing
was carried out to examine the composites’ mechanical behavior and
elasticity – important properties for soft tissue engineering materials.
Finally, the excellent antibacterial activity of the obtained silver/
poly(vinyl alcohol)/chitosan (Ag/PVA/CHI) and silver/poly(vinyl
alcohol)/chitosan/graphene (Ag/PVA/CHI/Gr) hydrogels against
Staphylococcus aureus and Escherichia coli was confirmed quantitatively
by colony counting method, and their non-toxicity was
verified towards two model fibroblast cell lines (MRC-5 and L929)
utilizing the MTT assay. Through thorough characterization, the
obtained nanocomposite hydrogel materials were identified as strong
candidates for active antibacterial soft tissue engineering materials.",
publisher = "Mary Ann Liebert, Inc.",
journal = "Tissue Engineering Part A",
title = "Silver Nanoparticles-Loaded Poly(Vinyl Alcohol)/Chitosan/Graphene Hydrogels Obtained by Electrochemical Synthesis",
number = "S1",
pages = "S-132",
volume = "28",
doi = "10.1089/ten.tea.2022.29025.abstracts"
}

Nešović, K., Janković, A., Surudžić, R., Vukašinović-Sekulić, M., Kojić, V., Radetić, T.,& Mišković-Stanković, V.. (2022). Silver Nanoparticles-Loaded Poly(Vinyl Alcohol)/Chitosan/Graphene Hydrogels Obtained by Electrochemical Synthesis. in Tissue Engineering Part A
Mary Ann Liebert, Inc.., 28(S1), S-132.
https://doi.org/10.1089/ten.tea.2022.29025.abstracts

Nešović K, Janković A, Surudžić R, Vukašinović-Sekulić M, Kojić V, Radetić T, Mišković-Stanković V. Silver Nanoparticles-Loaded Poly(Vinyl Alcohol)/Chitosan/Graphene Hydrogels Obtained by Electrochemical Synthesis. in Tissue Engineering Part A. 2022;28(S1):S-132.
doi:10.1089/ten.tea.2022.29025.abstracts .

Nešović, Katarina, Janković, Ana, Surudžić, Rade, Vukašinović-Sekulić, Maja, Kojić, Vesna, Radetić, Tamara, Mišković-Stanković, Vesna, "Silver Nanoparticles-Loaded Poly(Vinyl Alcohol)/Chitosan/Graphene Hydrogels Obtained by Electrochemical Synthesis" in Tissue Engineering Part A, 28, no. S1 (2022):S-132,
https://doi.org/10.1089/ten.tea.2022.29025.abstracts . .

TY  - CONF
AU  - Vukašinović Sekulić, Maja
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6077
AB  - Candida albicans is pathogenic yeast species present as a part of the normal microbiota mucosal surface oral and vaginal cavities and digestive tract of warm-blooded animals and humans, without causing any health problems. Intensive growth and infection can occur as a result of excessive use of broad-spectrum antibiotics, corticosteroids, in immunosuppressive individuals, so as under the influence of numerous external factors that weaken the body's defence system. Since antibiotics stimulate the growth of Candida albicans, treatment is based on the use of antimycotic drugs and dietary adjustments, when healing process can sometimes take several weeks. In addition, long-term use of antimycotic drugs causes side effects (nausea, vomiting, diarrhea) and their frequent use contributes development of resistant strains of Candida albicans, which further complicates curing. In order to alleviate treatment, recent years great interest is focused on finding and application of natural preparations, such as essential oils of spices and medicinal herbs, which possess numerous biological properties, including antifungal activity. The aim of this study was to evaluate the antifungal activity of essential oils of various spices and medicinal herbs against Candida albicans in order to find an alternative treatment for candidiasis. The antifungal activity of twenty essential oils and herbal tinctures was investigated by agar well diffusion and broth macrodilution methods, and the obtained results were compared with the effect of antimycotic drugs (nystatin) and commercial preparations present in the local marketplace. Based on the results obtained by the agar well diffusion method, undiluted essential oils and herbal tinctures can be divided into five groups, according to the diameter of inhibition zone they form. Very strong antifungal activity was observed with essential oils of oregano, basil, thyme and cinnamon, with a diameter of the inhibition zones greater than 40mm, while strong activity was also noticed with essential oil of lemon balm (34.5 mm). Essential oils of clove, ginger, mint, bay and lavender have shown moderate antifungal activity (20-30 mm) , while weak activity was registered with essential oils of rosemary, star anise and turmeric (16-20 mm). Very weak antifungal efficacy was observed with sage and immortelle essential oils (12-15 mm), while antifungal activity was not present with essential oils of frankincense and black pepper, as well with herbal tinctures of garlic and wild garlic. The lowest values for minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) were achieved with essential oils which had very strong and strong antifungal activity determined by agar well diffusion method, as undiluted and diluted in 96% ethanol. According to the results obtained in this study, the essential oils of cinnamon, oregano, basil, thyme and lemon balm can be used, instead of nystatin, in infections caused by Candida albicans strains.
PB  - Novi Sad : Matica srpska
C3  - Book of abstracts / The 7th international scientific meeting "Mycology, mycotoxicology, and mycoses", 2-3 June 2022, Novi Sad
T1  - Application of essential oils of spices and medicinal herbs against Candida albicans
SP  - 42
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6077
ER  - 

@conference{
author = "Vukašinović Sekulić, Maja",
year = "2022",
abstract = "Candida albicans is pathogenic yeast species present as a part of the normal microbiota mucosal surface oral and vaginal cavities and digestive tract of warm-blooded animals and humans, without causing any health problems. Intensive growth and infection can occur as a result of excessive use of broad-spectrum antibiotics, corticosteroids, in immunosuppressive individuals, so as under the influence of numerous external factors that weaken the body's defence system. Since antibiotics stimulate the growth of Candida albicans, treatment is based on the use of antimycotic drugs and dietary adjustments, when healing process can sometimes take several weeks. In addition, long-term use of antimycotic drugs causes side effects (nausea, vomiting, diarrhea) and their frequent use contributes development of resistant strains of Candida albicans, which further complicates curing. In order to alleviate treatment, recent years great interest is focused on finding and application of natural preparations, such as essential oils of spices and medicinal herbs, which possess numerous biological properties, including antifungal activity. The aim of this study was to evaluate the antifungal activity of essential oils of various spices and medicinal herbs against Candida albicans in order to find an alternative treatment for candidiasis. The antifungal activity of twenty essential oils and herbal tinctures was investigated by agar well diffusion and broth macrodilution methods, and the obtained results were compared with the effect of antimycotic drugs (nystatin) and commercial preparations present in the local marketplace. Based on the results obtained by the agar well diffusion method, undiluted essential oils and herbal tinctures can be divided into five groups, according to the diameter of inhibition zone they form. Very strong antifungal activity was observed with essential oils of oregano, basil, thyme and cinnamon, with a diameter of the inhibition zones greater than 40mm, while strong activity was also noticed with essential oil of lemon balm (34.5 mm). Essential oils of clove, ginger, mint, bay and lavender have shown moderate antifungal activity (20-30 mm) , while weak activity was registered with essential oils of rosemary, star anise and turmeric (16-20 mm). Very weak antifungal efficacy was observed with sage and immortelle essential oils (12-15 mm), while antifungal activity was not present with essential oils of frankincense and black pepper, as well with herbal tinctures of garlic and wild garlic. The lowest values for minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) were achieved with essential oils which had very strong and strong antifungal activity determined by agar well diffusion method, as undiluted and diluted in 96% ethanol. According to the results obtained in this study, the essential oils of cinnamon, oregano, basil, thyme and lemon balm can be used, instead of nystatin, in infections caused by Candida albicans strains.",
publisher = "Novi Sad : Matica srpska",
journal = "Book of abstracts / The 7th international scientific meeting "Mycology, mycotoxicology, and mycoses", 2-3 June 2022, Novi Sad",
title = "Application of essential oils of spices and medicinal herbs against Candida albicans",
pages = "42",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6077"
}

Vukašinović Sekulić, M.. (2022). Application of essential oils of spices and medicinal herbs against Candida albicans. in Book of abstracts / The 7th international scientific meeting "Mycology, mycotoxicology, and mycoses", 2-3 June 2022, Novi Sad
Novi Sad : Matica srpska., 42.
https://hdl.handle.net/21.15107/rcub_technorep_6077

Vukašinović Sekulić M. Application of essential oils of spices and medicinal herbs against Candida albicans. in Book of abstracts / The 7th international scientific meeting "Mycology, mycotoxicology, and mycoses", 2-3 June 2022, Novi Sad. 2022;:42.
https://hdl.handle.net/21.15107/rcub_technorep_6077 .

Vukašinović Sekulić, Maja, "Application of essential oils of spices and medicinal herbs against Candida albicans" in Book of abstracts / The 7th international scientific meeting "Mycology, mycotoxicology, and mycoses", 2-3 June 2022, Novi Sad (2022):42,
https://hdl.handle.net/21.15107/rcub_technorep_6077 .

TY  - JOUR
AU  - Jakovetić Tanasković, Sonja
AU  - Šekuljica, Nataša
AU  - Jovanović, Jelena
AU  - Gazikalović, Ivana
AU  - Grbavčić, Sanja
AU  - Đorđević, Nikola
AU  - Vukašinović-Sekulić, Maja
AU  - Hao, Jian
AU  - Luković, Nevena
AU  - Knežević-Jugović, Zorica
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4848
AB  - Wheat bran is a by-product of wheat flour production used as a nutritious and economic feed ingredient, but since it possesses several anti-nutritional factors that entrap nutrients and interfere with digestion and absorption, its application is limited. The utilization of organic-rich wastes for the production of value-added products and nutritional improvement of food is a means to an end - sustainability. Here we propose addressing these challenges via fermentation by Bacillus sp. TMF-2, a strain that produces several enzymes and simultaneously, wheat bran of higher nutritional quality. Following solid-state fermentation, the soluble phenolic content of wheat bran nearly triplicated compared to the raw bran. The total proportion of polyphenols along with the antioxidant capacity and free radical scavenging rate were significantly improved, particularly the ability to reduce Fe3+ increased by 10-fold. Additionally, the activity of hydrolytic enzymes: amylase, cellulase, pectinase, mannanase, protease, and phytase increased during fermentation, whereas the level of anti-nutrient, phytic acid decreased. Among all produced enzymes, there is a special emphasis on the protease prone to degrade gluten thus resulting in gluten-free wheat bran. The results of this research could contribute to a beneficial procedure for the natural modification of wheat bran, which further augments the health benefits and utilization of wheat bran.
PB  - Academic Press
T2  - Journal of Cereal Science
T1  - Upgrading of valuable food component contents and anti-nutritional factors depletion by solid-state fermentation: A way to valorize wheat bran for nutrition
SP  - 103159
VL  - 99
DO  - 10.1016/j.jcs.2020.103159
ER  - 

@article{
author = "Jakovetić Tanasković, Sonja and Šekuljica, Nataša and Jovanović, Jelena and Gazikalović, Ivana and Grbavčić, Sanja and Đorđević, Nikola and Vukašinović-Sekulić, Maja and Hao, Jian and Luković, Nevena and Knežević-Jugović, Zorica",
year = "2021",
abstract = "Wheat bran is a by-product of wheat flour production used as a nutritious and economic feed ingredient, but since it possesses several anti-nutritional factors that entrap nutrients and interfere with digestion and absorption, its application is limited. The utilization of organic-rich wastes for the production of value-added products and nutritional improvement of food is a means to an end - sustainability. Here we propose addressing these challenges via fermentation by Bacillus sp. TMF-2, a strain that produces several enzymes and simultaneously, wheat bran of higher nutritional quality. Following solid-state fermentation, the soluble phenolic content of wheat bran nearly triplicated compared to the raw bran. The total proportion of polyphenols along with the antioxidant capacity and free radical scavenging rate were significantly improved, particularly the ability to reduce Fe3+ increased by 10-fold. Additionally, the activity of hydrolytic enzymes: amylase, cellulase, pectinase, mannanase, protease, and phytase increased during fermentation, whereas the level of anti-nutrient, phytic acid decreased. Among all produced enzymes, there is a special emphasis on the protease prone to degrade gluten thus resulting in gluten-free wheat bran. The results of this research could contribute to a beneficial procedure for the natural modification of wheat bran, which further augments the health benefits and utilization of wheat bran.",
publisher = "Academic Press",
journal = "Journal of Cereal Science",
title = "Upgrading of valuable food component contents and anti-nutritional factors depletion by solid-state fermentation: A way to valorize wheat bran for nutrition",
pages = "103159",
volume = "99",
doi = "10.1016/j.jcs.2020.103159"
}

Jakovetić Tanasković, S., Šekuljica, N., Jovanović, J., Gazikalović, I., Grbavčić, S., Đorđević, N., Vukašinović-Sekulić, M., Hao, J., Luković, N.,& Knežević-Jugović, Z.. (2021). Upgrading of valuable food component contents and anti-nutritional factors depletion by solid-state fermentation: A way to valorize wheat bran for nutrition. in Journal of Cereal Science
Academic Press., 99, 103159.
https://doi.org/10.1016/j.jcs.2020.103159

Jakovetić Tanasković S, Šekuljica N, Jovanović J, Gazikalović I, Grbavčić S, Đorđević N, Vukašinović-Sekulić M, Hao J, Luković N, Knežević-Jugović Z. Upgrading of valuable food component contents and anti-nutritional factors depletion by solid-state fermentation: A way to valorize wheat bran for nutrition. in Journal of Cereal Science. 2021;99:103159.
doi:10.1016/j.jcs.2020.103159 .

Jakovetić Tanasković, Sonja, Šekuljica, Nataša, Jovanović, Jelena, Gazikalović, Ivana, Grbavčić, Sanja, Đorđević, Nikola, Vukašinović-Sekulić, Maja, Hao, Jian, Luković, Nevena, Knežević-Jugović, Zorica, "Upgrading of valuable food component contents and anti-nutritional factors depletion by solid-state fermentation: A way to valorize wheat bran for nutrition" in Journal of Cereal Science, 99 (2021):103159,
https://doi.org/10.1016/j.jcs.2020.103159 . .

TY  - JOUR
AU  - Volić, Mina M.
AU  - Đorđević, Verica B.
AU  - Vukašinović-Sekulić, Maja
AU  - Obradović, Nataša S.
AU  - Knežević-Jugović, Zorica D.
AU  - Bugarski, Branko M.
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5916
AB  - The aim of this study was to develop a stable hydrogel carrier system for thyme essential oil (TEO) that could protect its sensitive polyphenol compounds. The impact of wall material (soy protein and alginate) on encapsulation efficiency and thymol release in simulated gastrointestinal conditions, was investigated. The release of thymol was ~ 80 % and 20 % in simulated gastric and pancreatic solutions, respectively. Thyme essential oil plays an important role as an antimicrobial and antioxidant agent. Results indicated that encapsulated TEO inside the hydrogel matrix exhibited antioxidant activity demonstrated by CUPRAC and ABTS analysis, even after thermal treatment of the beads, indicating the metal chelate effect as dominant. In vitro antimicrobial activity of encapsulated TEO has been studied against several pathogenic microorganisms such as Escherichia coli, Staphylococcus aureus, Bacillus cereus and Candida albicans. Beads coded as Ca-A1.5/SP1.5 showed anti-Candida albicans activity, while modified bead formulations Ca-A1.5/SP1.5* and Ca-A1.5/SP0.25** showed bactericidal activity against Escherichia coli and Staphylococcus aureus.
PB  - University of Novi Sad, Faculty of Technology
T2  - Acta Periodica Technologica
T1  - ANTIOXIDANT and ANTIMICROBIAL CAPACITY of ENCAPSULATED THYME ESSENTIAL OIL in ALGINATE and SOY PROTEIN-BASED CARRIERS
EP  - 12
IS  - 52
SP  - 1
DO  - 10.2298/APT2152001V
ER  - 

@article{
author = "Volić, Mina M. and Đorđević, Verica B. and Vukašinović-Sekulić, Maja and Obradović, Nataša S. and Knežević-Jugović, Zorica D. and Bugarski, Branko M.",
year = "2021",
abstract = "The aim of this study was to develop a stable hydrogel carrier system for thyme essential oil (TEO) that could protect its sensitive polyphenol compounds. The impact of wall material (soy protein and alginate) on encapsulation efficiency and thymol release in simulated gastrointestinal conditions, was investigated. The release of thymol was ~ 80 % and 20 % in simulated gastric and pancreatic solutions, respectively. Thyme essential oil plays an important role as an antimicrobial and antioxidant agent. Results indicated that encapsulated TEO inside the hydrogel matrix exhibited antioxidant activity demonstrated by CUPRAC and ABTS analysis, even after thermal treatment of the beads, indicating the metal chelate effect as dominant. In vitro antimicrobial activity of encapsulated TEO has been studied against several pathogenic microorganisms such as Escherichia coli, Staphylococcus aureus, Bacillus cereus and Candida albicans. Beads coded as Ca-A1.5/SP1.5 showed anti-Candida albicans activity, while modified bead formulations Ca-A1.5/SP1.5* and Ca-A1.5/SP0.25** showed bactericidal activity against Escherichia coli and Staphylococcus aureus.",
publisher = "University of Novi Sad, Faculty of Technology",
journal = "Acta Periodica Technologica",
title = "ANTIOXIDANT and ANTIMICROBIAL CAPACITY of ENCAPSULATED THYME ESSENTIAL OIL in ALGINATE and SOY PROTEIN-BASED CARRIERS",
pages = "12-1",
number = "52",
doi = "10.2298/APT2152001V"
}

Volić, M. M., Đorđević, V. B., Vukašinović-Sekulić, M., Obradović, N. S., Knežević-Jugović, Z. D.,& Bugarski, B. M.. (2021). ANTIOXIDANT and ANTIMICROBIAL CAPACITY of ENCAPSULATED THYME ESSENTIAL OIL in ALGINATE and SOY PROTEIN-BASED CARRIERS. in Acta Periodica Technologica
University of Novi Sad, Faculty of Technology.(52), 1-12.
https://doi.org/10.2298/APT2152001V

Volić MM, Đorđević VB, Vukašinović-Sekulić M, Obradović NS, Knežević-Jugović ZD, Bugarski BM. ANTIOXIDANT and ANTIMICROBIAL CAPACITY of ENCAPSULATED THYME ESSENTIAL OIL in ALGINATE and SOY PROTEIN-BASED CARRIERS. in Acta Periodica Technologica. 2021;(52):1-12.
doi:10.2298/APT2152001V .

Volić, Mina M., Đorđević, Verica B., Vukašinović-Sekulić, Maja, Obradović, Nataša S., Knežević-Jugović, Zorica D., Bugarski, Branko M., "ANTIOXIDANT and ANTIMICROBIAL CAPACITY of ENCAPSULATED THYME ESSENTIAL OIL in ALGINATE and SOY PROTEIN-BASED CARRIERS" in Acta Periodica Technologica, no. 52 (2021):1-12,
https://doi.org/10.2298/APT2152001V . .

TY  - CONF
AU  - Vukašinović-Sekulić, Maja
AU  - Mitrić, Aleksandra
AU  - Bulatović, Maja
AU  - Rakin, Marica
AU  - Krunić, Tanja
PY  - 2020
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6114
AB  - Background: In the recent years, food industry have been intensively working to replace chemical preservatives with natural, effective and nontoxic compounds which could inactivate or inhibit growth of spoilage and pathogenic microorganisms. Among natural antimicrobial agents, extracts and essential oils derived from plant and spices, in addition to contributing to the taste and flavor of food, also can reduce or eliminate a wide variety of bacterial species.
Objectives: Purpose of this study was to evaluate the antibacterial effect of clove essential oil in milk against foodborne pathogenic and spoilage bacteria, which is a frequent cause of milk spoilage and food-borne infection and intoxication worldwide.
Methods: Antibacterial activity of clove essential oil in milk with different content of milk fat (0.5 and 3.2% w/v) was examined by agar well diffusion method, while the influence of milk components on minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) were estimated by macrodilution method.
Results: The data obtained by an agar well diffusion method confirmed that milk is good medium for clove essential oil dissolution, especially milk with 0,5% milk fat, as inhibition zones were noticed for Bacillus subtilis up to 1,5% (v/v), Escherichia coli, Salmonella Enteritidis, Shigella sonnei, Listeria monocytogenes till 3,1% (v/v), while as a most resistant were Staphylococcus aureus, Bacillus cereus and Listeria innocua (6,2% v/v). Lower values for MIC and MBC were no-
ticed in milk with 0,5% milk fat, which were almost three to five fold higher values from that achieved in nutrient broth, depending of bacterial species tested.
PB  - Federation of European Microbiological Societies
C3  - Federation of European Microbiological Societies
T1  - Antibacterial activity of clove essential oil in milk against food-borne pathogenic and spoilage bacteria
SP  - 162
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6114
ER  - 

@conference{
author = "Vukašinović-Sekulić, Maja and Mitrić, Aleksandra and Bulatović, Maja and Rakin, Marica and Krunić, Tanja",
year = "2020",
abstract = "Background: In the recent years, food industry have been intensively working to replace chemical preservatives with natural, effective and nontoxic compounds which could inactivate or inhibit growth of spoilage and pathogenic microorganisms. Among natural antimicrobial agents, extracts and essential oils derived from plant and spices, in addition to contributing to the taste and flavor of food, also can reduce or eliminate a wide variety of bacterial species.
Objectives: Purpose of this study was to evaluate the antibacterial effect of clove essential oil in milk against foodborne pathogenic and spoilage bacteria, which is a frequent cause of milk spoilage and food-borne infection and intoxication worldwide.
Methods: Antibacterial activity of clove essential oil in milk with different content of milk fat (0.5 and 3.2% w/v) was examined by agar well diffusion method, while the influence of milk components on minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) were estimated by macrodilution method.
Results: The data obtained by an agar well diffusion method confirmed that milk is good medium for clove essential oil dissolution, especially milk with 0,5% milk fat, as inhibition zones were noticed for Bacillus subtilis up to 1,5% (v/v), Escherichia coli, Salmonella Enteritidis, Shigella sonnei, Listeria monocytogenes till 3,1% (v/v), while as a most resistant were Staphylococcus aureus, Bacillus cereus and Listeria innocua (6,2% v/v). Lower values for MIC and MBC were no-
ticed in milk with 0,5% milk fat, which were almost three to five fold higher values from that achieved in nutrient broth, depending of bacterial species tested.",
publisher = "Federation of European Microbiological Societies",
journal = "Federation of European Microbiological Societies",
title = "Antibacterial activity of clove essential oil in milk against food-borne pathogenic and spoilage bacteria",
pages = "162",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6114"
}

Vukašinović-Sekulić, M., Mitrić, A., Bulatović, M., Rakin, M.,& Krunić, T.. (2020). Antibacterial activity of clove essential oil in milk against food-borne pathogenic and spoilage bacteria. in Federation of European Microbiological Societies
Federation of European Microbiological Societies., 162.
https://hdl.handle.net/21.15107/rcub_technorep_6114

Vukašinović-Sekulić M, Mitrić A, Bulatović M, Rakin M, Krunić T. Antibacterial activity of clove essential oil in milk against food-borne pathogenic and spoilage bacteria. in Federation of European Microbiological Societies. 2020;:162.
https://hdl.handle.net/21.15107/rcub_technorep_6114 .

Vukašinović-Sekulić, Maja, Mitrić, Aleksandra, Bulatović, Maja, Rakin, Marica, Krunić, Tanja, "Antibacterial activity of clove essential oil in milk against food-borne pathogenic and spoilage bacteria" in Federation of European Microbiological Societies (2020):162,
https://hdl.handle.net/21.15107/rcub_technorep_6114 .

TY  - JOUR
AU  - Nešović, Katarina
AU  - Janković, Ana
AU  - Radetić, Tamara
AU  - Perić-Grujić, Aleksandra
AU  - Vukašinović-Sekulić, Maja
AU  - Kojić, Vesna
AU  - Rhee, Kyong Yop
AU  - Mišković-Stanković, Vesna
PY  - 2020
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4495
AB  - Polymer-based hydrogel materials are excellent candidates for new-generation wound dressings with improved properties, such as high sorption ability, good mechanical properties and low adhesiveness. Cross-linked hydrogel matrices also serve as excellent carriers for controlled release of antibacterial agents, such as silver nanoparticles (AgNPs), which are preferred over conventional antibiotics due to low propensity to induce bacterial resistance. In this work, we aim to produce novel silver/poly(vinyl alcohol)/chitosan (Ag/PVA/CHI) hydrogels for wound dressing applications. The electrochemical AgNPs synthesis provided facile and green method for the reduction of Ag+ ions inside the hydrogel matrices, without the need to use toxic chemical reducing agents. The formation of AgNPs was confirmed using UV-visible spectroscopy, scanning and transmission electron microscopy. Release kinetics was investigated in modified phosphate buffer solution at 37 degrees C to mimic physiological conditions. Release profiles indicated "burst release" behavior, which is beneficial for wound dressing applications. The antibacterial activity was evaluated against Staphylococcus aureus and Escherichia coli strains using disc-diffusion test, and non-toxicity of hydrogels was proved by dye-exclusion test. The obtained results confirmed strong potential of Ag/PVA/CHI hydrogels for biomedical applications.
PB  - Int Assoc Physical Chemists-Iapc, Zagreb
T2  - Journal of Electrochemical Science and Engineering
T1  - Poly(vinyl alcohol)/chitosan hydrogels with electrochemically synthesized silver nanoparticles for wound dressing applications
EP  - 198
IS  - 2
SP  - 185
VL  - 10
DO  - 10.5599/jese.732
ER  - 

@article{
author = "Nešović, Katarina and Janković, Ana and Radetić, Tamara and Perić-Grujić, Aleksandra and Vukašinović-Sekulić, Maja and Kojić, Vesna and Rhee, Kyong Yop and Mišković-Stanković, Vesna",
year = "2020",
abstract = "Polymer-based hydrogel materials are excellent candidates for new-generation wound dressings with improved properties, such as high sorption ability, good mechanical properties and low adhesiveness. Cross-linked hydrogel matrices also serve as excellent carriers for controlled release of antibacterial agents, such as silver nanoparticles (AgNPs), which are preferred over conventional antibiotics due to low propensity to induce bacterial resistance. In this work, we aim to produce novel silver/poly(vinyl alcohol)/chitosan (Ag/PVA/CHI) hydrogels for wound dressing applications. The electrochemical AgNPs synthesis provided facile and green method for the reduction of Ag+ ions inside the hydrogel matrices, without the need to use toxic chemical reducing agents. The formation of AgNPs was confirmed using UV-visible spectroscopy, scanning and transmission electron microscopy. Release kinetics was investigated in modified phosphate buffer solution at 37 degrees C to mimic physiological conditions. Release profiles indicated "burst release" behavior, which is beneficial for wound dressing applications. The antibacterial activity was evaluated against Staphylococcus aureus and Escherichia coli strains using disc-diffusion test, and non-toxicity of hydrogels was proved by dye-exclusion test. The obtained results confirmed strong potential of Ag/PVA/CHI hydrogels for biomedical applications.",
publisher = "Int Assoc Physical Chemists-Iapc, Zagreb",
journal = "Journal of Electrochemical Science and Engineering",
title = "Poly(vinyl alcohol)/chitosan hydrogels with electrochemically synthesized silver nanoparticles for wound dressing applications",
pages = "198-185",
number = "2",
volume = "10",
doi = "10.5599/jese.732"
}

Nešović, K., Janković, A., Radetić, T., Perić-Grujić, A., Vukašinović-Sekulić, M., Kojić, V., Rhee, K. Y.,& Mišković-Stanković, V.. (2020). Poly(vinyl alcohol)/chitosan hydrogels with electrochemically synthesized silver nanoparticles for wound dressing applications. in Journal of Electrochemical Science and Engineering
Int Assoc Physical Chemists-Iapc, Zagreb., 10(2), 185-198.
https://doi.org/10.5599/jese.732

Nešović K, Janković A, Radetić T, Perić-Grujić A, Vukašinović-Sekulić M, Kojić V, Rhee KY, Mišković-Stanković V. Poly(vinyl alcohol)/chitosan hydrogels with electrochemically synthesized silver nanoparticles for wound dressing applications. in Journal of Electrochemical Science and Engineering. 2020;10(2):185-198.
doi:10.5599/jese.732 .

Nešović, Katarina, Janković, Ana, Radetić, Tamara, Perić-Grujić, Aleksandra, Vukašinović-Sekulić, Maja, Kojić, Vesna, Rhee, Kyong Yop, Mišković-Stanković, Vesna, "Poly(vinyl alcohol)/chitosan hydrogels with electrochemically synthesized silver nanoparticles for wound dressing applications" in Journal of Electrochemical Science and Engineering, 10, no. 2 (2020):185-198,
https://doi.org/10.5599/jese.732 . .

TY  - JOUR
AU  - Stevanović, Milena
AU  - Đošić, Marija
AU  - Janković, Ana
AU  - Kojić, Vesna
AU  - Vukašinović-Sekulić, Maja
AU  - Stojanović, Jovica
AU  - Odović, Jadranka
AU  - Crevar-Sakač, Milkica
AU  - Kyong Yop, Rhee
AU  - Mišković-Stanković, Vesna
PY  - 2020
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4501
AB  - Electrophoretic deposition process (EPD) was successfully used for obtaining graphene (Gr)-reinforced composite coating based on hydroxyapatite (HAP), chitosan (CS), and antibiotic gentamicin (Gent), from aqueous suspension. The deposition process was performed as a single step process at a constant voltage (5 V, deposition time 12 min) on pure titanium foils. The influence of graphene was examined through detailed physicochemical and biological characterization. Fourier transform infrared spectroscopy, field emission scanning electron microscopy, thermogravimetric analysis, X-ray diffraction, Raman, and X-ray photoelectron analyses confirmed the formation of composite HAP/CS/Gr and HAP/CS/Gr/Gent coatings on Ti. Obtained coatings had porous, uniform, fracture-free surfaces, suggesting strong interfacial interaction between HAP, CS, and Gr. Large specific area of graphene enabled strong bonding with chitosan, acting as nanofiller throughout the polymer matrix. Gentamicin addition strongly improved the antibacterial activity of HAP/CS/Gr/Gent coating that was confirmed by antibacterial activity kinetics in suspension and agar diffusion testing, while results indicated more pronounced antibacterial effect againstStaphylococcus aureus(bactericidal, viable cells number reduction  gt 3 logarithmic units) compared toEscherichia coli(bacteriostatic, lt 3 logarithmic units).MTT assay indicated low cytotoxicity (75% cell viability) against MRC-5 and L929 (70% cell viability) tested cell lines, indicating good biocompatibility of HAP/CS/Gr/Gent coating. Therefore, electrodeposited HAP/CS/Gr/Gent coating on Ti can be considered as a prospective material for bone tissue engineering as a hard tissue implant.
PB  - Wiley, Hoboken
T2  - Journal of Biomedical Materials Research Part A
T1  - Antibacterial graphene-based hydroxyapatite/chitosan coating with gentamicin for potential applications in bone tissue engineering
EP  - 2189
IS  - 11
SP  - 2175
VL  - 108
DO  - 10.1002/jbm.a.36974
ER  - 

@article{
author = "Stevanović, Milena and Đošić, Marija and Janković, Ana and Kojić, Vesna and Vukašinović-Sekulić, Maja and Stojanović, Jovica and Odović, Jadranka and Crevar-Sakač, Milkica and Kyong Yop, Rhee and Mišković-Stanković, Vesna",
year = "2020",
abstract = "Electrophoretic deposition process (EPD) was successfully used for obtaining graphene (Gr)-reinforced composite coating based on hydroxyapatite (HAP), chitosan (CS), and antibiotic gentamicin (Gent), from aqueous suspension. The deposition process was performed as a single step process at a constant voltage (5 V, deposition time 12 min) on pure titanium foils. The influence of graphene was examined through detailed physicochemical and biological characterization. Fourier transform infrared spectroscopy, field emission scanning electron microscopy, thermogravimetric analysis, X-ray diffraction, Raman, and X-ray photoelectron analyses confirmed the formation of composite HAP/CS/Gr and HAP/CS/Gr/Gent coatings on Ti. Obtained coatings had porous, uniform, fracture-free surfaces, suggesting strong interfacial interaction between HAP, CS, and Gr. Large specific area of graphene enabled strong bonding with chitosan, acting as nanofiller throughout the polymer matrix. Gentamicin addition strongly improved the antibacterial activity of HAP/CS/Gr/Gent coating that was confirmed by antibacterial activity kinetics in suspension and agar diffusion testing, while results indicated more pronounced antibacterial effect againstStaphylococcus aureus(bactericidal, viable cells number reduction  gt 3 logarithmic units) compared toEscherichia coli(bacteriostatic, lt 3 logarithmic units).MTT assay indicated low cytotoxicity (75% cell viability) against MRC-5 and L929 (70% cell viability) tested cell lines, indicating good biocompatibility of HAP/CS/Gr/Gent coating. Therefore, electrodeposited HAP/CS/Gr/Gent coating on Ti can be considered as a prospective material for bone tissue engineering as a hard tissue implant.",
publisher = "Wiley, Hoboken",
journal = "Journal of Biomedical Materials Research Part A",
title = "Antibacterial graphene-based hydroxyapatite/chitosan coating with gentamicin for potential applications in bone tissue engineering",
pages = "2189-2175",
number = "11",
volume = "108",
doi = "10.1002/jbm.a.36974"
}

Stevanović, M., Đošić, M., Janković, A., Kojić, V., Vukašinović-Sekulić, M., Stojanović, J., Odović, J., Crevar-Sakač, M., Kyong Yop, R.,& Mišković-Stanković, V.. (2020). Antibacterial graphene-based hydroxyapatite/chitosan coating with gentamicin for potential applications in bone tissue engineering. in Journal of Biomedical Materials Research Part A
Wiley, Hoboken., 108(11), 2175-2189.
https://doi.org/10.1002/jbm.a.36974

Stevanović M, Đošić M, Janković A, Kojić V, Vukašinović-Sekulić M, Stojanović J, Odović J, Crevar-Sakač M, Kyong Yop R, Mišković-Stanković V. Antibacterial graphene-based hydroxyapatite/chitosan coating with gentamicin for potential applications in bone tissue engineering. in Journal of Biomedical Materials Research Part A. 2020;108(11):2175-2189.
doi:10.1002/jbm.a.36974 .

Stevanović, Milena, Đošić, Marija, Janković, Ana, Kojić, Vesna, Vukašinović-Sekulić, Maja, Stojanović, Jovica, Odović, Jadranka, Crevar-Sakač, Milkica, Kyong Yop, Rhee, Mišković-Stanković, Vesna, "Antibacterial graphene-based hydroxyapatite/chitosan coating with gentamicin for potential applications in bone tissue engineering" in Journal of Biomedical Materials Research Part A, 108, no. 11 (2020):2175-2189,
https://doi.org/10.1002/jbm.a.36974 . .

TY  - JOUR
AU  - Stevanović, Milena
AU  - Đošić, Marija
AU  - Janković, Ana
AU  - Kojić, Vesna
AU  - Vukašinović-Sekulić, Maja
AU  - Stojanović, Jovica
AU  - Odović, Jadranka
AU  - Crevar-Sakač, Milkica
AU  - Kyong Yop, Rhee
AU  - Mišković-Stanković, Vesna
PY  - 2020
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4725
AB  - Electrophoretic deposition process (EPD) was successfully used for obtaining graphene (Gr)-reinforced composite coating based on hydroxyapatite (HAP), chitosan (CS), and antibiotic gentamicin (Gent), from aqueous suspension. The deposition process was performed as a single step process at a constant voltage (5 V, deposition time 12 min) on pure titanium foils. The influence of graphene was examined through detailed physicochemical and biological characterization. Fourier transform infrared spectroscopy, field emission scanning electron microscopy, thermogravimetric analysis, X-ray diffraction, Raman, and X-ray photoelectron analyses confirmed the formation of composite HAP/CS/Gr and HAP/CS/Gr/Gent coatings on Ti. Obtained coatings had porous, uniform, fracture-free surfaces, suggesting strong interfacial interaction between HAP, CS, and Gr. Large specific area of graphene enabled strong bonding with chitosan, acting as nanofiller throughout the polymer matrix. Gentamicin addition strongly improved the antibacterial activity of HAP/CS/Gr/Gent coating that was confirmed by antibacterial activity kinetics in suspension and agar diffusion testing, while results indicated more pronounced antibacterial effect againstStaphylococcus aureus(bactericidal, viable cells number reduction  gt 3 logarithmic units) compared toEscherichia coli(bacteriostatic, lt 3 logarithmic units).MTT assay indicated low cytotoxicity (75% cell viability) against MRC-5 and L929 (70% cell viability) tested cell lines, indicating good biocompatibility of HAP/CS/Gr/Gent coating. Therefore, electrodeposited HAP/CS/Gr/Gent coating on Ti can be considered as a prospective material for bone tissue engineering as a hard tissue implant.
PB  - Wiley, Hoboken
T2  - Journal of Biomedical Materials Research Part A
T1  - Antibacterial graphene-based hydroxyapatite/chitosan coating with gentamicin for potential applications in bone tissue engineering
EP  - 2189
IS  - 11
SP  - 2175
VL  - 108
DO  - 10.1002/jbm.a.36974
ER  - 

@article{
author = "Stevanović, Milena and Đošić, Marija and Janković, Ana and Kojić, Vesna and Vukašinović-Sekulić, Maja and Stojanović, Jovica and Odović, Jadranka and Crevar-Sakač, Milkica and Kyong Yop, Rhee and Mišković-Stanković, Vesna",
year = "2020",
abstract = "Electrophoretic deposition process (EPD) was successfully used for obtaining graphene (Gr)-reinforced composite coating based on hydroxyapatite (HAP), chitosan (CS), and antibiotic gentamicin (Gent), from aqueous suspension. The deposition process was performed as a single step process at a constant voltage (5 V, deposition time 12 min) on pure titanium foils. The influence of graphene was examined through detailed physicochemical and biological characterization. Fourier transform infrared spectroscopy, field emission scanning electron microscopy, thermogravimetric analysis, X-ray diffraction, Raman, and X-ray photoelectron analyses confirmed the formation of composite HAP/CS/Gr and HAP/CS/Gr/Gent coatings on Ti. Obtained coatings had porous, uniform, fracture-free surfaces, suggesting strong interfacial interaction between HAP, CS, and Gr. Large specific area of graphene enabled strong bonding with chitosan, acting as nanofiller throughout the polymer matrix. Gentamicin addition strongly improved the antibacterial activity of HAP/CS/Gr/Gent coating that was confirmed by antibacterial activity kinetics in suspension and agar diffusion testing, while results indicated more pronounced antibacterial effect againstStaphylococcus aureus(bactericidal, viable cells number reduction  gt 3 logarithmic units) compared toEscherichia coli(bacteriostatic, lt 3 logarithmic units).MTT assay indicated low cytotoxicity (75% cell viability) against MRC-5 and L929 (70% cell viability) tested cell lines, indicating good biocompatibility of HAP/CS/Gr/Gent coating. Therefore, electrodeposited HAP/CS/Gr/Gent coating on Ti can be considered as a prospective material for bone tissue engineering as a hard tissue implant.",
publisher = "Wiley, Hoboken",
journal = "Journal of Biomedical Materials Research Part A",
title = "Antibacterial graphene-based hydroxyapatite/chitosan coating with gentamicin for potential applications in bone tissue engineering",
pages = "2189-2175",
number = "11",
volume = "108",
doi = "10.1002/jbm.a.36974"
}

Stevanović, M., Đošić, M., Janković, A., Kojić, V., Vukašinović-Sekulić, M., Stojanović, J., Odović, J., Crevar-Sakač, M., Kyong Yop, R.,& Mišković-Stanković, V.. (2020). Antibacterial graphene-based hydroxyapatite/chitosan coating with gentamicin for potential applications in bone tissue engineering. in Journal of Biomedical Materials Research Part A
Wiley, Hoboken., 108(11), 2175-2189.
https://doi.org/10.1002/jbm.a.36974

Stevanović M, Đošić M, Janković A, Kojić V, Vukašinović-Sekulić M, Stojanović J, Odović J, Crevar-Sakač M, Kyong Yop R, Mišković-Stanković V. Antibacterial graphene-based hydroxyapatite/chitosan coating with gentamicin for potential applications in bone tissue engineering. in Journal of Biomedical Materials Research Part A. 2020;108(11):2175-2189.
doi:10.1002/jbm.a.36974 .

Stevanović, Milena, Đošić, Marija, Janković, Ana, Kojić, Vesna, Vukašinović-Sekulić, Maja, Stojanović, Jovica, Odović, Jadranka, Crevar-Sakač, Milkica, Kyong Yop, Rhee, Mišković-Stanković, Vesna, "Antibacterial graphene-based hydroxyapatite/chitosan coating with gentamicin for potential applications in bone tissue engineering" in Journal of Biomedical Materials Research Part A, 108, no. 11 (2020):2175-2189,
https://doi.org/10.1002/jbm.a.36974 . .

TY  - CONF
AU  - Vukašinović-Sekulić, Maja
AU  - Rakin, Marica
AU  - Bulatović, Maja
AU  - Krunić, Tanja
PY  - 2019
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6116
AB  - Object and goals of the study: Clove essential oil is a clear, colourless to yellow liquid obtained from dried aromatic unopened floral buds of an evergreen tree Syzygium aromaticum. It possess strong characteristic sweet and spicy odour and a warm, almost burning and spicy flavour, wherefore it has been used as a flavouring agent in food industries. Beside influence on sensory properties of foods, it has also antimicrobial and antioxidant activities and can be used as a natural food additive, instead of chemical preservatives, to extend products shelf-life. Many intrinsic properties of the food (fat/protein/water content, antioxidants, preservatives, pH, salt and other additives), as also extrinsic determinants (temperature, packaging in vacuum/gas/air, characteristics of microorganisms) can have impact on antimicrobial activity of essential oils. The objective of this study was to assess influence of organic matter on the antibacterial activity of clove essential oil against Staphylococcus aureus, one of food-borne pathogenic bacteria species which is frequent cause of food infection and intoxication worldwide. 
The experimental methods applied: Antibacterial activity of clove oil was determined by the agar well diffusion and broth macrodilution methods against two strains of Staphylococcus aureus, with a different sensitivity to the most frequently used antibiotics. Clove oil was purchased from the local market and before screening antibacterial activity it was dissolved in appropriate concentration in sterilized physiological saline solution (0.85% w/v) supplemented with Tween 80 at final concentration of 0.5% (v/v), nutrition broth and milk with different content of milk fat (0.5 and 3.2% w/v) and total solid (4.5 and 9% w/v). Influence of organic matter on the antibacterial activity of clove essential oil was estimated based on diameter of inhibition zones, minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC).
Results obtained: The results of antibacterial activity obtained by agar well diffusion method have indicated that clove essential oil is better dissolved in milk samples with 0.5% and 3.2% (w/v) milk fat than in nutrition broth and physiological saline solution with Tween 80, since with these samples clear zones of inhibition were noticed till clove oil concentration 3.1% (v/v) and 6.2 (v/v), respectively. On the other hand, the results of broth macrodilution method have shown that the lowest values for MIC and MBC could be achieved when clove oil is added in physiological saline solution with Tween 80, afterwards both values have progressively increased, as content of organic matter in a media was higher.  
Conclusions: Presence of high levels of fat and/or protein in foodstuffs could protect the microbial cells from the action of the essential oils, which is the reason that a greater concentration of essential oils is needed to achieve the same effect in foods, compared to laboratory media. Also, greater availability of nutrients in foods may enable microorganisms to repair damaged cells faster and to make effect of application of essential oils in food matrices more unpredictable.
PB  - Faculty of Technology, Leskovac
C3  - Faculty of Technology, Leskovac
T1  - Influence of organic matter on the antibacterial activity of clove essential oil against Staphylococcus aureus
SP  - 59
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6116
ER  - 

@conference{
author = "Vukašinović-Sekulić, Maja and Rakin, Marica and Bulatović, Maja and Krunić, Tanja",
year = "2019",
abstract = "Object and goals of the study: Clove essential oil is a clear, colourless to yellow liquid obtained from dried aromatic unopened floral buds of an evergreen tree Syzygium aromaticum. It possess strong characteristic sweet and spicy odour and a warm, almost burning and spicy flavour, wherefore it has been used as a flavouring agent in food industries. Beside influence on sensory properties of foods, it has also antimicrobial and antioxidant activities and can be used as a natural food additive, instead of chemical preservatives, to extend products shelf-life. Many intrinsic properties of the food (fat/protein/water content, antioxidants, preservatives, pH, salt and other additives), as also extrinsic determinants (temperature, packaging in vacuum/gas/air, characteristics of microorganisms) can have impact on antimicrobial activity of essential oils. The objective of this study was to assess influence of organic matter on the antibacterial activity of clove essential oil against Staphylococcus aureus, one of food-borne pathogenic bacteria species which is frequent cause of food infection and intoxication worldwide. 
The experimental methods applied: Antibacterial activity of clove oil was determined by the agar well diffusion and broth macrodilution methods against two strains of Staphylococcus aureus, with a different sensitivity to the most frequently used antibiotics. Clove oil was purchased from the local market and before screening antibacterial activity it was dissolved in appropriate concentration in sterilized physiological saline solution (0.85% w/v) supplemented with Tween 80 at final concentration of 0.5% (v/v), nutrition broth and milk with different content of milk fat (0.5 and 3.2% w/v) and total solid (4.5 and 9% w/v). Influence of organic matter on the antibacterial activity of clove essential oil was estimated based on diameter of inhibition zones, minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC).
Results obtained: The results of antibacterial activity obtained by agar well diffusion method have indicated that clove essential oil is better dissolved in milk samples with 0.5% and 3.2% (w/v) milk fat than in nutrition broth and physiological saline solution with Tween 80, since with these samples clear zones of inhibition were noticed till clove oil concentration 3.1% (v/v) and 6.2 (v/v), respectively. On the other hand, the results of broth macrodilution method have shown that the lowest values for MIC and MBC could be achieved when clove oil is added in physiological saline solution with Tween 80, afterwards both values have progressively increased, as content of organic matter in a media was higher.  
Conclusions: Presence of high levels of fat and/or protein in foodstuffs could protect the microbial cells from the action of the essential oils, which is the reason that a greater concentration of essential oils is needed to achieve the same effect in foods, compared to laboratory media. Also, greater availability of nutrients in foods may enable microorganisms to repair damaged cells faster and to make effect of application of essential oils in food matrices more unpredictable.",
publisher = "Faculty of Technology, Leskovac",
journal = "Faculty of Technology, Leskovac",
title = "Influence of organic matter on the antibacterial activity of clove essential oil against Staphylococcus aureus",
pages = "59",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6116"
}

Vukašinović-Sekulić, M., Rakin, M., Bulatović, M.,& Krunić, T.. (2019). Influence of organic matter on the antibacterial activity of clove essential oil against Staphylococcus aureus. in Faculty of Technology, Leskovac
Faculty of Technology, Leskovac., 59.
https://hdl.handle.net/21.15107/rcub_technorep_6116

Vukašinović-Sekulić M, Rakin M, Bulatović M, Krunić T. Influence of organic matter on the antibacterial activity of clove essential oil against Staphylococcus aureus. in Faculty of Technology, Leskovac. 2019;:59.
https://hdl.handle.net/21.15107/rcub_technorep_6116 .

Vukašinović-Sekulić, Maja, Rakin, Marica, Bulatović, Maja, Krunić, Tanja, "Influence of organic matter on the antibacterial activity of clove essential oil against Staphylococcus aureus" in Faculty of Technology, Leskovac (2019):59,
https://hdl.handle.net/21.15107/rcub_technorep_6116 .

TY  - JOUR
AU  - Nešović, Katarina
AU  - Janković, Ana
AU  - Perić-Grujić, Aleksandra
AU  - Vukašinović-Sekulić, Maja
AU  - Radetić, Tamara
AU  - Živković, Ljiljana
AU  - Park, Soo Jin
AU  - Rhee, Kyong Yop
AU  - Mišković-Stanković, Vesna
PY  - 2019
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4239
AB  - Silver nanoparticles (AgNPs) were synthesized by in situ electrochemical reduction of Ag+ ions in poly (vinyl alcohol)/chitosan/graphene (PVA/CHI/Gr) hydrogel matrices with different concentrations of chitosan. The physicochemical properties of nanocomposite hydrogels were investigated by UV-vis spectroscopy (UV-vis), dynamic light scattering (DLS), transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), field-emission scanning electron microscopy (FE-SEM) and Fourier transform infrared spectroscopy (FT-IR), thermal characteristics were determined by differential scanning calorimetry (DSC) and mechanical properties were measured by tensile test. The swelling studies were carried out in phosphate buffer to simulate natural physiological environment and data were fitted by several kinetic models to determine the diffusion mechanism and diffusion coefficients of the swelling medium through the hydrogel matrices. It was shown that the presence of silver nanoparticles increased the uptake capability and equilibrium swelling degree of the composite hydrogels. The antibacterial activity was confirmed against Escherichia coli and Staphylococcus aureus, while the hydrogels without AgNPs exhibited antibacterial properties due to the presence of chitosan. With the addition of AgNPs, the samples showed stronger activity and fast reduction in the number of colonies, confirming the synergistic effect of chitosan and AgNPs on the antibacterial activity.
PB  - Elsevier Science Inc, New York
T2  - Journal of Industrial and Engineering Chemistry
T1  - Kinetic models of swelling and thermal stability of silver/poly(vinyl Check far alcohol)/chitosan/graphene hydrogels
EP  - 96
SP  - 83
VL  - 77
DO  - 10.1016/j.jiec.2019.04.022
ER  - 

@article{
author = "Nešović, Katarina and Janković, Ana and Perić-Grujić, Aleksandra and Vukašinović-Sekulić, Maja and Radetić, Tamara and Živković, Ljiljana and Park, Soo Jin and Rhee, Kyong Yop and Mišković-Stanković, Vesna",
year = "2019",
abstract = "Silver nanoparticles (AgNPs) were synthesized by in situ electrochemical reduction of Ag+ ions in poly (vinyl alcohol)/chitosan/graphene (PVA/CHI/Gr) hydrogel matrices with different concentrations of chitosan. The physicochemical properties of nanocomposite hydrogels were investigated by UV-vis spectroscopy (UV-vis), dynamic light scattering (DLS), transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), field-emission scanning electron microscopy (FE-SEM) and Fourier transform infrared spectroscopy (FT-IR), thermal characteristics were determined by differential scanning calorimetry (DSC) and mechanical properties were measured by tensile test. The swelling studies were carried out in phosphate buffer to simulate natural physiological environment and data were fitted by several kinetic models to determine the diffusion mechanism and diffusion coefficients of the swelling medium through the hydrogel matrices. It was shown that the presence of silver nanoparticles increased the uptake capability and equilibrium swelling degree of the composite hydrogels. The antibacterial activity was confirmed against Escherichia coli and Staphylococcus aureus, while the hydrogels without AgNPs exhibited antibacterial properties due to the presence of chitosan. With the addition of AgNPs, the samples showed stronger activity and fast reduction in the number of colonies, confirming the synergistic effect of chitosan and AgNPs on the antibacterial activity.",
publisher = "Elsevier Science Inc, New York",
journal = "Journal of Industrial and Engineering Chemistry",
title = "Kinetic models of swelling and thermal stability of silver/poly(vinyl Check far alcohol)/chitosan/graphene hydrogels",
pages = "96-83",
volume = "77",
doi = "10.1016/j.jiec.2019.04.022"
}

Nešović, K., Janković, A., Perić-Grujić, A., Vukašinović-Sekulić, M., Radetić, T., Živković, L., Park, S. J., Rhee, K. Y.,& Mišković-Stanković, V.. (2019). Kinetic models of swelling and thermal stability of silver/poly(vinyl Check far alcohol)/chitosan/graphene hydrogels. in Journal of Industrial and Engineering Chemistry
Elsevier Science Inc, New York., 77, 83-96.
https://doi.org/10.1016/j.jiec.2019.04.022

Nešović K, Janković A, Perić-Grujić A, Vukašinović-Sekulić M, Radetić T, Živković L, Park SJ, Rhee KY, Mišković-Stanković V. Kinetic models of swelling and thermal stability of silver/poly(vinyl Check far alcohol)/chitosan/graphene hydrogels. in Journal of Industrial and Engineering Chemistry. 2019;77:83-96.
doi:10.1016/j.jiec.2019.04.022 .

Nešović, Katarina, Janković, Ana, Perić-Grujić, Aleksandra, Vukašinović-Sekulić, Maja, Radetić, Tamara, Živković, Ljiljana, Park, Soo Jin, Rhee, Kyong Yop, Mišković-Stanković, Vesna, "Kinetic models of swelling and thermal stability of silver/poly(vinyl Check far alcohol)/chitosan/graphene hydrogels" in Journal of Industrial and Engineering Chemistry, 77 (2019):83-96,
https://doi.org/10.1016/j.jiec.2019.04.022 . .

TY  - JOUR
AU  - Nešović, Katarina
AU  - Janković, Ana
AU  - Perić-Grujić, Aleksandra
AU  - Vukašinović-Sekulić, Maja
AU  - Radetić, Tamara
AU  - Živković, Ljiljana
AU  - Park, Soo Jin
AU  - Rhee, Kyong Yop
AU  - Mišković-Stanković, Vesna
PY  - 2019
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5044
AB  - Silver nanoparticles (AgNPs) were synthesized by in situ electrochemical reduction of Ag+ ions in poly(vinyl alcohol)/chitosan/graphene (PVA/CHI/Gr) hydrogel matrices with different concentrations of chitosan. The physicochemical properties of nanocomposite hydrogels were investigated by UV–vis spectroscopy (UV–vis), dynamic light scattering (DLS), transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), field-emission scanning electron microscopy (FE-SEM) and Fourier-transform infrared spectroscopy (FT-IR), thermal characteristics were determined by differential scanning calorimetry (DSC) and mechanical properties were measured by tensile test. The swelling studies were carried out in phosphate buffer to simulate natural physiological environment and data were fitted by several kinetic models to determine the diffusion mechanism and diffusion coefficients of the swelling medium through the hydrogel matrices. It was shown that the presence of silver nanoparticles increased the uptake capability and equilibrium swelling degree of the composite hydrogels. The antibacterial activity was confirmed against Escherichia coli and Staphylococcus aureus, while the hydrogels without AgNPs exhibited antibacterial properties due to the presence of chitosan. With the addition of AgNPs, the samples showed stronger activity and fast reduction in the number of colonies, confirming the synergistic effect of chitosan and AgNPs on the antibacterial activity.
PB  - Korean Society of Industrial Engineering Chemistry
T2  - Journal of Industrial and Engineering Chemistry
T1  - Kinetic models of swelling and thermal stability of silver/poly(vinyl alcohol)/chitosan/graphene hydrogels
EP  - 96
SP  - 83
VL  - 77
DO  - 10.1016/j.jiec.2019.04.022
ER  - 

@article{
author = "Nešović, Katarina and Janković, Ana and Perić-Grujić, Aleksandra and Vukašinović-Sekulić, Maja and Radetić, Tamara and Živković, Ljiljana and Park, Soo Jin and Rhee, Kyong Yop and Mišković-Stanković, Vesna",
year = "2019",
abstract = "Silver nanoparticles (AgNPs) were synthesized by in situ electrochemical reduction of Ag+ ions in poly(vinyl alcohol)/chitosan/graphene (PVA/CHI/Gr) hydrogel matrices with different concentrations of chitosan. The physicochemical properties of nanocomposite hydrogels were investigated by UV–vis spectroscopy (UV–vis), dynamic light scattering (DLS), transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), field-emission scanning electron microscopy (FE-SEM) and Fourier-transform infrared spectroscopy (FT-IR), thermal characteristics were determined by differential scanning calorimetry (DSC) and mechanical properties were measured by tensile test. The swelling studies were carried out in phosphate buffer to simulate natural physiological environment and data were fitted by several kinetic models to determine the diffusion mechanism and diffusion coefficients of the swelling medium through the hydrogel matrices. It was shown that the presence of silver nanoparticles increased the uptake capability and equilibrium swelling degree of the composite hydrogels. The antibacterial activity was confirmed against Escherichia coli and Staphylococcus aureus, while the hydrogels without AgNPs exhibited antibacterial properties due to the presence of chitosan. With the addition of AgNPs, the samples showed stronger activity and fast reduction in the number of colonies, confirming the synergistic effect of chitosan and AgNPs on the antibacterial activity.",
publisher = "Korean Society of Industrial Engineering Chemistry",
journal = "Journal of Industrial and Engineering Chemistry",
title = "Kinetic models of swelling and thermal stability of silver/poly(vinyl alcohol)/chitosan/graphene hydrogels",
pages = "96-83",
volume = "77",
doi = "10.1016/j.jiec.2019.04.022"
}

Nešović, K., Janković, A., Perić-Grujić, A., Vukašinović-Sekulić, M., Radetić, T., Živković, L., Park, S. J., Rhee, K. Y.,& Mišković-Stanković, V.. (2019). Kinetic models of swelling and thermal stability of silver/poly(vinyl alcohol)/chitosan/graphene hydrogels. in Journal of Industrial and Engineering Chemistry
Korean Society of Industrial Engineering Chemistry., 77, 83-96.
https://doi.org/10.1016/j.jiec.2019.04.022

Nešović K, Janković A, Perić-Grujić A, Vukašinović-Sekulić M, Radetić T, Živković L, Park SJ, Rhee KY, Mišković-Stanković V. Kinetic models of swelling and thermal stability of silver/poly(vinyl alcohol)/chitosan/graphene hydrogels. in Journal of Industrial and Engineering Chemistry. 2019;77:83-96.
doi:10.1016/j.jiec.2019.04.022 .

Nešović, Katarina, Janković, Ana, Perić-Grujić, Aleksandra, Vukašinović-Sekulić, Maja, Radetić, Tamara, Živković, Ljiljana, Park, Soo Jin, Rhee, Kyong Yop, Mišković-Stanković, Vesna, "Kinetic models of swelling and thermal stability of silver/poly(vinyl alcohol)/chitosan/graphene hydrogels" in Journal of Industrial and Engineering Chemistry, 77 (2019):83-96,
https://doi.org/10.1016/j.jiec.2019.04.022 . .

TY  - CONF
AU  - Mišković-Stanković, Vesna
AU  - Nešović, Katarina
AU  - Janković, Ana
AU  - Radetić, Tamara
AU  - Perić-Grujić, Aleksandra
AU  - Vukašinović-Sekulić, Maja
AU  - Kojić, Vesna
AU  - Yop Rhee, Kyong
PY  - 2019
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7245
AB  - In recent times, polymer-based hydrogel materials have presented themselves as excellent candidates for
new-generation wound dressings with improved properties, such as high sorption ability, good mechanical
properties and low adhesiveness [1]. Additionally, cross linked hydrogel matrices serve as excellent carriers
for controlled release of antibacterial agents, such as silver nanoparticles (Ag/NPs), which are preferred over
conventional antibiotics due to multi-phase mechanism of action and low susceptibility to induce bacterial
resistance [2]. In this work, we aim to produce novel silver/poly(vinyl alcohol)/chitosan (Ag/PVA/CHI)
hydrogels for wound dressing applications. The electrochemical route for AgNPs synthesis provided facile
and green method for the reduction of Ag+
ions inside the hydrogel matrices, without the need to use toxic
chemical reducing agents [3]. The effect of chitosan content on the synthesis yield, antibacterial properties,
swelling and release kinetics was investigated. The formation of AgNPs was confirmed using UV-visible
spectroscopy through the appearance of plasmon resonant peaks at around 400 nm (Figure 1a), whereas
transmission electron microscopy (TEM) proved the incorporation of both single and polycrystalline spherical
AgNPs with diameters of 5-10 nm (Figure 1b). The swelling and silver release kinetics were investigated in
modified phosphate buffer solutions (pH 7.4) at 37 °C to mimic physiological conditions. The obtained
swelling isotherms and release profiles were fitted with different models to obtain kinetic and diffusion
parameters (Figure 1c). The antibacterial activity was evaluated against Staphylococcus aureus TL and
Escherichia coli ATCC 25922 bacterial strains using an in-suspension test, and non-toxicity of both silvercontaining and silver-free hydrogels was proved by MTT cytotoxicity test. The obtained results confirmed
strong potential of Ag/PVA/CHI hydrogels for biomedical applications.
PB  - International Association of Physical Chemists
C3  - Book of abstracts / 7th Regional Symposium on Electrochemistry – South East Europe & 8th Kurt Schwabe Symposium, Split, Croatia, May 27-30, 2019
T1  - Poly(vinyl alcohol)/chitosan hydrogels with electrochemically synthesized silver nanoparticles for wound dressing applications
SP  - 121
UR  - https://hdl.handle.net/21.15107/rcub_technorep_7245
ER  - 

@conference{
author = "Mišković-Stanković, Vesna and Nešović, Katarina and Janković, Ana and Radetić, Tamara and Perić-Grujić, Aleksandra and Vukašinović-Sekulić, Maja and Kojić, Vesna and Yop Rhee, Kyong",
year = "2019",
abstract = "In recent times, polymer-based hydrogel materials have presented themselves as excellent candidates for
new-generation wound dressings with improved properties, such as high sorption ability, good mechanical
properties and low adhesiveness [1]. Additionally, cross linked hydrogel matrices serve as excellent carriers
for controlled release of antibacterial agents, such as silver nanoparticles (Ag/NPs), which are preferred over
conventional antibiotics due to multi-phase mechanism of action and low susceptibility to induce bacterial
resistance [2]. In this work, we aim to produce novel silver/poly(vinyl alcohol)/chitosan (Ag/PVA/CHI)
hydrogels for wound dressing applications. The electrochemical route for AgNPs synthesis provided facile
and green method for the reduction of Ag+
ions inside the hydrogel matrices, without the need to use toxic
chemical reducing agents [3]. The effect of chitosan content on the synthesis yield, antibacterial properties,
swelling and release kinetics was investigated. The formation of AgNPs was confirmed using UV-visible
spectroscopy through the appearance of plasmon resonant peaks at around 400 nm (Figure 1a), whereas
transmission electron microscopy (TEM) proved the incorporation of both single and polycrystalline spherical
AgNPs with diameters of 5-10 nm (Figure 1b). The swelling and silver release kinetics were investigated in
modified phosphate buffer solutions (pH 7.4) at 37 °C to mimic physiological conditions. The obtained
swelling isotherms and release profiles were fitted with different models to obtain kinetic and diffusion
parameters (Figure 1c). The antibacterial activity was evaluated against Staphylococcus aureus TL and
Escherichia coli ATCC 25922 bacterial strains using an in-suspension test, and non-toxicity of both silvercontaining and silver-free hydrogels was proved by MTT cytotoxicity test. The obtained results confirmed
strong potential of Ag/PVA/CHI hydrogels for biomedical applications.",
publisher = "International Association of Physical Chemists",
journal = "Book of abstracts / 7th Regional Symposium on Electrochemistry – South East Europe & 8th Kurt Schwabe Symposium, Split, Croatia, May 27-30, 2019",
title = "Poly(vinyl alcohol)/chitosan hydrogels with electrochemically synthesized silver nanoparticles for wound dressing applications",
pages = "121",
url = "https://hdl.handle.net/21.15107/rcub_technorep_7245"
}

Mišković-Stanković, V., Nešović, K., Janković, A., Radetić, T., Perić-Grujić, A., Vukašinović-Sekulić, M., Kojić, V.,& Yop Rhee, K.. (2019). Poly(vinyl alcohol)/chitosan hydrogels with electrochemically synthesized silver nanoparticles for wound dressing applications. in Book of abstracts / 7th Regional Symposium on Electrochemistry – South East Europe & 8th Kurt Schwabe Symposium, Split, Croatia, May 27-30, 2019
International Association of Physical Chemists., 121.
https://hdl.handle.net/21.15107/rcub_technorep_7245

Mišković-Stanković V, Nešović K, Janković A, Radetić T, Perić-Grujić A, Vukašinović-Sekulić M, Kojić V, Yop Rhee K. Poly(vinyl alcohol)/chitosan hydrogels with electrochemically synthesized silver nanoparticles for wound dressing applications. in Book of abstracts / 7th Regional Symposium on Electrochemistry – South East Europe & 8th Kurt Schwabe Symposium, Split, Croatia, May 27-30, 2019. 2019;:121.
https://hdl.handle.net/21.15107/rcub_technorep_7245 .

Mišković-Stanković, Vesna, Nešović, Katarina, Janković, Ana, Radetić, Tamara, Perić-Grujić, Aleksandra, Vukašinović-Sekulić, Maja, Kojić, Vesna, Yop Rhee, Kyong, "Poly(vinyl alcohol)/chitosan hydrogels with electrochemically synthesized silver nanoparticles for wound dressing applications" in Book of abstracts / 7th Regional Symposium on Electrochemistry – South East Europe & 8th Kurt Schwabe Symposium, Split, Croatia, May 27-30, 2019 (2019):121,
https://hdl.handle.net/21.15107/rcub_technorep_7245 .

TY  - JOUR
AU  - Salim, Abdalla Ali
AU  - Grbavčić, Sanja
AU  - Šekuljica, Nataša
AU  - Vukašinović-Sekulić, Maja
AU  - Jovanović, Jelena
AU  - Jakovetić Tanasković, Sonja
AU  - Luković, Nevena
AU  - Knežević-Jugović, Zorica
PY  - 2019
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4065
AB  - Protease, cellulase, and alpha-amylase producing Bacillus subtilis strain was cultivated by solid-state fermentation technique using soybean meal as a substrate. The aim of the present study was to establish a highly efficient enzymes' extraction method as a first stage in downstream processing. The conventional extraction procedure was optimized by determining pH, stirring rate, solid/liquid ratio, and time of extraction on enzymes' recoveries from fermented soybean meal. Yields of leached enzymes were compared to the amounts of enzymes that are achieved with ultrasound-assisted extraction (UAE). UAE was established to be superior method for obtaining higher yields of proteases (up to 330 IU) and alpha-amylases (825 IU), under significantly shorter extraction time and gaining more concentrated product. However, the obtained model predicts that conventional process led to a product with a higher cellulolytic activity ( gt = 7.5 IU).
PB  - Wiley, Hoboken
T2  - Biotechnology and Applied Biochemistry
T1  - Enzyme production by solid-state fermentation on soybean meal: A comparative study of conventional and ultrasound-assisted extraction methods
EP  - 368
IS  - 3
SP  - 361
VL  - 66
DO  - 10.1002/bab.1732
ER  - 

@article{
author = "Salim, Abdalla Ali and Grbavčić, Sanja and Šekuljica, Nataša and Vukašinović-Sekulić, Maja and Jovanović, Jelena and Jakovetić Tanasković, Sonja and Luković, Nevena and Knežević-Jugović, Zorica",
year = "2019",
abstract = "Protease, cellulase, and alpha-amylase producing Bacillus subtilis strain was cultivated by solid-state fermentation technique using soybean meal as a substrate. The aim of the present study was to establish a highly efficient enzymes' extraction method as a first stage in downstream processing. The conventional extraction procedure was optimized by determining pH, stirring rate, solid/liquid ratio, and time of extraction on enzymes' recoveries from fermented soybean meal. Yields of leached enzymes were compared to the amounts of enzymes that are achieved with ultrasound-assisted extraction (UAE). UAE was established to be superior method for obtaining higher yields of proteases (up to 330 IU) and alpha-amylases (825 IU), under significantly shorter extraction time and gaining more concentrated product. However, the obtained model predicts that conventional process led to a product with a higher cellulolytic activity ( gt = 7.5 IU).",
publisher = "Wiley, Hoboken",
journal = "Biotechnology and Applied Biochemistry",
title = "Enzyme production by solid-state fermentation on soybean meal: A comparative study of conventional and ultrasound-assisted extraction methods",
pages = "368-361",
number = "3",
volume = "66",
doi = "10.1002/bab.1732"
}

Salim, A. A., Grbavčić, S., Šekuljica, N., Vukašinović-Sekulić, M., Jovanović, J., Jakovetić Tanasković, S., Luković, N.,& Knežević-Jugović, Z.. (2019). Enzyme production by solid-state fermentation on soybean meal: A comparative study of conventional and ultrasound-assisted extraction methods. in Biotechnology and Applied Biochemistry
Wiley, Hoboken., 66(3), 361-368.
https://doi.org/10.1002/bab.1732

Salim AA, Grbavčić S, Šekuljica N, Vukašinović-Sekulić M, Jovanović J, Jakovetić Tanasković S, Luković N, Knežević-Jugović Z. Enzyme production by solid-state fermentation on soybean meal: A comparative study of conventional and ultrasound-assisted extraction methods. in Biotechnology and Applied Biochemistry. 2019;66(3):361-368.
doi:10.1002/bab.1732 .

Salim, Abdalla Ali, Grbavčić, Sanja, Šekuljica, Nataša, Vukašinović-Sekulić, Maja, Jovanović, Jelena, Jakovetić Tanasković, Sonja, Luković, Nevena, Knežević-Jugović, Zorica, "Enzyme production by solid-state fermentation on soybean meal: A comparative study of conventional and ultrasound-assisted extraction methods" in Biotechnology and Applied Biochemistry, 66, no. 3 (2019):361-368,
https://doi.org/10.1002/bab.1732 . .

TY  - JOUR
AU  - Nešović, Katarina
AU  - Janković, Ana
AU  - Radetić, Tamara
AU  - Vukašinović-Sekulić, Maja
AU  - Kojić, Vesna
AU  - Živković, Ljiljana
AU  - Perić-Grujić, Aleksandra
AU  - Rhee, Kyong Yop
AU  - Mišković-Stanković, Vesna
PY  - 2019
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4113
AB  - In this work, we provide a facile path for the preparation of wound dressing materials with improved characteristics based on biocompatible chitosan and poly(vinyl alcohol) hydrogels with embedded silver nanoparticles (AgNPs) as a potent antimicrobial agent. The hydrogels were prepared via freezing-thawing method, whereas AgNPs were synthesized via an in situ electrochemical route. The excellent physicochemical properties of the obtained nanocomposite hydrogels were validated using different spectroscopic and microscopic techniques. The formation of sub-10 nm AgNPs was confirmed by UV-visible spectroscopy, dynamic light scattering and transmission electron microscopy. Swelling and silver release profiles were validated using several diffusion models. The hydrogels were proven to be non-cytotoxic using MTT test and possessed powerful antibacterial activity against Staphylococcus aureus and Escherichia coli, as determined by test in suspension, which affirmed their feasibility for wound dressing applications.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - European Polymer Journal
T1  - Chitosan-based hydrogel wound dressings with electrochemically incorporated silver nanoparticles - In vitro study
VL  - 121
DO  - 10.1016/j.eurpolymj.2019.109257
ER  - 

@article{
author = "Nešović, Katarina and Janković, Ana and Radetić, Tamara and Vukašinović-Sekulić, Maja and Kojić, Vesna and Živković, Ljiljana and Perić-Grujić, Aleksandra and Rhee, Kyong Yop and Mišković-Stanković, Vesna",
year = "2019",
abstract = "In this work, we provide a facile path for the preparation of wound dressing materials with improved characteristics based on biocompatible chitosan and poly(vinyl alcohol) hydrogels with embedded silver nanoparticles (AgNPs) as a potent antimicrobial agent. The hydrogels were prepared via freezing-thawing method, whereas AgNPs were synthesized via an in situ electrochemical route. The excellent physicochemical properties of the obtained nanocomposite hydrogels were validated using different spectroscopic and microscopic techniques. The formation of sub-10 nm AgNPs was confirmed by UV-visible spectroscopy, dynamic light scattering and transmission electron microscopy. Swelling and silver release profiles were validated using several diffusion models. The hydrogels were proven to be non-cytotoxic using MTT test and possessed powerful antibacterial activity against Staphylococcus aureus and Escherichia coli, as determined by test in suspension, which affirmed their feasibility for wound dressing applications.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "European Polymer Journal",
title = "Chitosan-based hydrogel wound dressings with electrochemically incorporated silver nanoparticles - In vitro study",
volume = "121",
doi = "10.1016/j.eurpolymj.2019.109257"
}

Nešović, K., Janković, A., Radetić, T., Vukašinović-Sekulić, M., Kojić, V., Živković, L., Perić-Grujić, A., Rhee, K. Y.,& Mišković-Stanković, V.. (2019). Chitosan-based hydrogel wound dressings with electrochemically incorporated silver nanoparticles - In vitro study. in European Polymer Journal
Pergamon-Elsevier Science Ltd, Oxford., 121.
https://doi.org/10.1016/j.eurpolymj.2019.109257

Nešović K, Janković A, Radetić T, Vukašinović-Sekulić M, Kojić V, Živković L, Perić-Grujić A, Rhee KY, Mišković-Stanković V. Chitosan-based hydrogel wound dressings with electrochemically incorporated silver nanoparticles - In vitro study. in European Polymer Journal. 2019;121.
doi:10.1016/j.eurpolymj.2019.109257 .

Nešović, Katarina, Janković, Ana, Radetić, Tamara, Vukašinović-Sekulić, Maja, Kojić, Vesna, Živković, Ljiljana, Perić-Grujić, Aleksandra, Rhee, Kyong Yop, Mišković-Stanković, Vesna, "Chitosan-based hydrogel wound dressings with electrochemically incorporated silver nanoparticles - In vitro study" in European Polymer Journal, 121 (2019),
https://doi.org/10.1016/j.eurpolymj.2019.109257 . .

TY  - JOUR
AU  - Kostić, Danijela
AU  - Vukašinović-Sekulić, Maja
AU  - Armentano, Ilaria
AU  - Torres, Luigi
AU  - Obradović, Bojana
PY  - 2019
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4161
AB  - Novel multifunctional composite poly(lactic acid) (PLA) films with alginate microbeads containing silver nanoparticles (AgNPs) were developed for potential antimicrobial food packaging applications. AgNPs, 10-20 nm in size, were synthesized in a Na-alginate solution by a hydrothermal method yielding a sterile, pH neutral colloid solution of low viscosity that was electrostatically extruded to produce Ag/alginate microbeads (190 mu m in size) with retained AgNPs. Dried microbeads were uniformly dispersed in PLA films with retained AgNPs as confirmed by UV-Vis spectroscopy and scanning electron microscopy. The films were characterized regarding thermal and mechanical properties as well as silver release in different food simulants. Results show that PLA matrix served as a diffusion barrier so that the released silver concentration in water after 10 days was within the prescribed limit of 0.05 mg kg(-1) while the films induced inhibitory effects against Staphylococcus aureus in the agar diffusion test.
PB  - Elsevier Science Bv, Amsterdam
T2  - Materials Science & Engineering C-Materials for Biological Applications
T1  - Multifunctional ternary composite films based on PLA and Ag/alginate microbeads: Physical characterization and silver release kinetics
EP  - 1168
SP  - 1159
VL  - 98
DO  - 10.1016/j.msec.2019.01.074
ER  - 

@article{
author = "Kostić, Danijela and Vukašinović-Sekulić, Maja and Armentano, Ilaria and Torres, Luigi and Obradović, Bojana",
year = "2019",
abstract = "Novel multifunctional composite poly(lactic acid) (PLA) films with alginate microbeads containing silver nanoparticles (AgNPs) were developed for potential antimicrobial food packaging applications. AgNPs, 10-20 nm in size, were synthesized in a Na-alginate solution by a hydrothermal method yielding a sterile, pH neutral colloid solution of low viscosity that was electrostatically extruded to produce Ag/alginate microbeads (190 mu m in size) with retained AgNPs. Dried microbeads were uniformly dispersed in PLA films with retained AgNPs as confirmed by UV-Vis spectroscopy and scanning electron microscopy. The films were characterized regarding thermal and mechanical properties as well as silver release in different food simulants. Results show that PLA matrix served as a diffusion barrier so that the released silver concentration in water after 10 days was within the prescribed limit of 0.05 mg kg(-1) while the films induced inhibitory effects against Staphylococcus aureus in the agar diffusion test.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Materials Science & Engineering C-Materials for Biological Applications",
title = "Multifunctional ternary composite films based on PLA and Ag/alginate microbeads: Physical characterization and silver release kinetics",
pages = "1168-1159",
volume = "98",
doi = "10.1016/j.msec.2019.01.074"
}

Kostić, D., Vukašinović-Sekulić, M., Armentano, I., Torres, L.,& Obradović, B.. (2019). Multifunctional ternary composite films based on PLA and Ag/alginate microbeads: Physical characterization and silver release kinetics. in Materials Science & Engineering C-Materials for Biological Applications
Elsevier Science Bv, Amsterdam., 98, 1159-1168.
https://doi.org/10.1016/j.msec.2019.01.074

Kostić D, Vukašinović-Sekulić M, Armentano I, Torres L, Obradović B. Multifunctional ternary composite films based on PLA and Ag/alginate microbeads: Physical characterization and silver release kinetics. in Materials Science & Engineering C-Materials for Biological Applications. 2019;98:1159-1168.
doi:10.1016/j.msec.2019.01.074 .

Kostić, Danijela, Vukašinović-Sekulić, Maja, Armentano, Ilaria, Torres, Luigi, Obradović, Bojana, "Multifunctional ternary composite films based on PLA and Ag/alginate microbeads: Physical characterization and silver release kinetics" in Materials Science & Engineering C-Materials for Biological Applications, 98 (2019):1159-1168,
https://doi.org/10.1016/j.msec.2019.01.074 . .

TY  - CONF
AU  - Nešović, Katarina
AU  - Janković, Ana
AU  - Vukašinović-Sekulić, Maja
AU  - Perić-Grujić, Aleksandra
AU  - Mišković-Stanković, Vesna
PY  - 2018
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7386
AB  - In this work, we prepared hydrogels based on PVA, CHI and Gr, and used them
as a porous matrix to synthesize silver nanoparticles by an in situ electrochemical
method [3,4]. The hydrogels were prepared from PVA/CHI/Gr colloid dispersions
using a simple and effective freezing-thawing method for gelation, without the use
of chemical cross linkers. The hydrogels were then swollen in AgNO3 solution to
saturate with Ag+ ions and subsequently subjected to constant-voltage electrical
current, to reduce silver ions and to obtain AgNPs. This electrochemical method
is fast and green, as there is no need for the use of any (potentially toxic) chemical
cross linkers and reducing agents, rendering the final product non-toxic and safe for
medical applications.
PB  - Belgrade : Serbian Academy of Sciences and Arts
C3  - Program ; Book of Abstracts / First International Conference on Electron Microscopy of Nanostructures ELMINA 2018, August 27-29, 2018, Belgrade, Serbia = Прва међународна конференција о електронској микроскопији наноструктура ELMINA 2018, 27-29 август 2018. Београд, Србија
T1  - Nanocomposite Hydrogels Based on Poly(vinyl alcohol) and Chitosan with Silver Nanoparticles and Graphene Aimed for Wound Dressing Applications
EP  - 242
SP  - 240
UR  - https://hdl.handle.net/21.15107/rcub_technorep_7386
ER  - 

@conference{
author = "Nešović, Katarina and Janković, Ana and Vukašinović-Sekulić, Maja and Perić-Grujić, Aleksandra and Mišković-Stanković, Vesna",
year = "2018",
abstract = "In this work, we prepared hydrogels based on PVA, CHI and Gr, and used them
as a porous matrix to synthesize silver nanoparticles by an in situ electrochemical
method [3,4]. The hydrogels were prepared from PVA/CHI/Gr colloid dispersions
using a simple and effective freezing-thawing method for gelation, without the use
of chemical cross linkers. The hydrogels were then swollen in AgNO3 solution to
saturate with Ag+ ions and subsequently subjected to constant-voltage electrical
current, to reduce silver ions and to obtain AgNPs. This electrochemical method
is fast and green, as there is no need for the use of any (potentially toxic) chemical
cross linkers and reducing agents, rendering the final product non-toxic and safe for
medical applications.",
publisher = "Belgrade : Serbian Academy of Sciences and Arts",
journal = "Program ; Book of Abstracts / First International Conference on Electron Microscopy of Nanostructures ELMINA 2018, August 27-29, 2018, Belgrade, Serbia = Прва међународна конференција о електронској микроскопији наноструктура ELMINA 2018, 27-29 август 2018. Београд, Србија",
title = "Nanocomposite Hydrogels Based on Poly(vinyl alcohol) and Chitosan with Silver Nanoparticles and Graphene Aimed for Wound Dressing Applications",
pages = "242-240",
url = "https://hdl.handle.net/21.15107/rcub_technorep_7386"
}

Nešović, K., Janković, A., Vukašinović-Sekulić, M., Perić-Grujić, A.,& Mišković-Stanković, V.. (2018). Nanocomposite Hydrogels Based on Poly(vinyl alcohol) and Chitosan with Silver Nanoparticles and Graphene Aimed for Wound Dressing Applications. in Program ; Book of Abstracts / First International Conference on Electron Microscopy of Nanostructures ELMINA 2018, August 27-29, 2018, Belgrade, Serbia = Прва међународна конференција о електронској микроскопији наноструктура ELMINA 2018, 27-29 август 2018. Београд, Србија
Belgrade : Serbian Academy of Sciences and Arts., 240-242.
https://hdl.handle.net/21.15107/rcub_technorep_7386

Nešović K, Janković A, Vukašinović-Sekulić M, Perić-Grujić A, Mišković-Stanković V. Nanocomposite Hydrogels Based on Poly(vinyl alcohol) and Chitosan with Silver Nanoparticles and Graphene Aimed for Wound Dressing Applications. in Program ; Book of Abstracts / First International Conference on Electron Microscopy of Nanostructures ELMINA 2018, August 27-29, 2018, Belgrade, Serbia = Прва међународна конференција о електронској микроскопији наноструктура ELMINA 2018, 27-29 август 2018. Београд, Србија. 2018;:240-242.
https://hdl.handle.net/21.15107/rcub_technorep_7386 .

Nešović, Katarina, Janković, Ana, Vukašinović-Sekulić, Maja, Perić-Grujić, Aleksandra, Mišković-Stanković, Vesna, "Nanocomposite Hydrogels Based on Poly(vinyl alcohol) and Chitosan with Silver Nanoparticles and Graphene Aimed for Wound Dressing Applications" in Program ; Book of Abstracts / First International Conference on Electron Microscopy of Nanostructures ELMINA 2018, August 27-29, 2018, Belgrade, Serbia = Прва међународна конференција о електронској микроскопији наноструктура ELMINA 2018, 27-29 август 2018. Београд, Србија (2018):240-242,
https://hdl.handle.net/21.15107/rcub_technorep_7386 .

TY  - JOUR
AU  - Embiriekah, Salem
AU  - Bulatović, Maja
AU  - Gnjatović, Marija Lj
AU  - Vukašinović-Sekulić, Maja
AU  - Krunić, Tanja
AU  - Zarić, Danica
AU  - Rakin, Marica
PY  - 2018
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3926
AB  - The aim of this study was to examine the bioactive potential of hydrolysate powders produced by enzymatic and microbial hydrolysis of whey proteins followed by spray drying, in order to reveal which one of these processes result in a product with significantly improved functional properties. Hydrolysate powders produced by the two different biotechnological processes were compared based on their antioxidant (DPPH and FTC), antibacterial as well as erythrocyte membrane stabilizing activities. The performed tests revealed that the concentration of at least 178.4 mg mL(-1) of the whey protein hydrolysate powder, produced by tryptic digestion, could inhibit the process of lipid peroxidation by 50 %, suppress the microbial contamination caused by S. aureus ATCC25923, B. cereus ATCC 11778 and L. monocytogenes, and provide the antioxidant and membrane stabilizing activities greater than 50 %. On the other hand, the hydrolysate powder obtained by whey fermentation at the concentration of at least 811.5 mg mL(-1) achieved 50 % of all tested bioactivities, with the emphasis on the significantly more pronounced antibacterial activity against all tested strains. In that sense, tryptic hydrolysis could be highlighted as an optimal process that provides production of the whey hydrolysate with pronounced bioactive properties that could be considered as a very promising natural food supplement.
PB  - Savez hemijskih inženjera, Beograd
T2  - Hemijska industrija
T1  - Comparative analysis of functionality of spray dried whey protein hydrolysates obtained by enzymatic and microbial hydrolysis
EP  - 274
IS  - 5
SP  - 265
VL  - 72
DO  - 10.2298/HEMIND171121019E
ER  - 

@article{
author = "Embiriekah, Salem and Bulatović, Maja and Gnjatović, Marija Lj and Vukašinović-Sekulić, Maja and Krunić, Tanja and Zarić, Danica and Rakin, Marica",
year = "2018",
abstract = "The aim of this study was to examine the bioactive potential of hydrolysate powders produced by enzymatic and microbial hydrolysis of whey proteins followed by spray drying, in order to reveal which one of these processes result in a product with significantly improved functional properties. Hydrolysate powders produced by the two different biotechnological processes were compared based on their antioxidant (DPPH and FTC), antibacterial as well as erythrocyte membrane stabilizing activities. The performed tests revealed that the concentration of at least 178.4 mg mL(-1) of the whey protein hydrolysate powder, produced by tryptic digestion, could inhibit the process of lipid peroxidation by 50 %, suppress the microbial contamination caused by S. aureus ATCC25923, B. cereus ATCC 11778 and L. monocytogenes, and provide the antioxidant and membrane stabilizing activities greater than 50 %. On the other hand, the hydrolysate powder obtained by whey fermentation at the concentration of at least 811.5 mg mL(-1) achieved 50 % of all tested bioactivities, with the emphasis on the significantly more pronounced antibacterial activity against all tested strains. In that sense, tryptic hydrolysis could be highlighted as an optimal process that provides production of the whey hydrolysate with pronounced bioactive properties that could be considered as a very promising natural food supplement.",
publisher = "Savez hemijskih inženjera, Beograd",
journal = "Hemijska industrija",
title = "Comparative analysis of functionality of spray dried whey protein hydrolysates obtained by enzymatic and microbial hydrolysis",
pages = "274-265",
number = "5",
volume = "72",
doi = "10.2298/HEMIND171121019E"
}

Embiriekah, S., Bulatović, M., Gnjatović, M. L., Vukašinović-Sekulić, M., Krunić, T., Zarić, D.,& Rakin, M.. (2018). Comparative analysis of functionality of spray dried whey protein hydrolysates obtained by enzymatic and microbial hydrolysis. in Hemijska industrija
Savez hemijskih inženjera, Beograd., 72(5), 265-274.
https://doi.org/10.2298/HEMIND171121019E

Embiriekah S, Bulatović M, Gnjatović ML, Vukašinović-Sekulić M, Krunić T, Zarić D, Rakin M. Comparative analysis of functionality of spray dried whey protein hydrolysates obtained by enzymatic and microbial hydrolysis. in Hemijska industrija. 2018;72(5):265-274.
doi:10.2298/HEMIND171121019E .

Embiriekah, Salem, Bulatović, Maja, Gnjatović, Marija Lj, Vukašinović-Sekulić, Maja, Krunić, Tanja, Zarić, Danica, Rakin, Marica, "Comparative analysis of functionality of spray dried whey protein hydrolysates obtained by enzymatic and microbial hydrolysis" in Hemijska industrija, 72, no. 5 (2018):265-274,
https://doi.org/10.2298/HEMIND171121019E . .

TY  - CONF
AU  - Vukašinović-Sekulić, Maja
AU  - Bulatović, Maja
AU  - Rakin, Marica
AU  - Krunić, Tanja
PY  - 2018
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6120
AB  - INTRODUCTION: Food infection and intoxication are still a major problem worldwide, in spite of the modern methods used to preserve food. Application of synthetic preservatives may lead to negative health consequences and growing public interest is now focussing toward discovery of novel substances from natural sources which will be safer and without any side effects. Among natural antimicrobial agents, spices could be promising alternative to synthetic additives, since at the same time they enhance aroma and flavour and control food-borne pathogens and spoilage bacteria in processed foods. Clove (Syzygium aromaticum) is aromatic spice commonly used in the cuisine of Asian, African, and the Near and Middle East countries. It has many biological properties which mainly derived from high level of eugenol (72-90%) in its essential oil. AIM: The purpose of this study was to evaluate antibacterial activity of commercial clove essential oil against a range of food-born pathogenic bacteria such as Escherichia coli, Salmonella enteritidis, Shigella sonnei, Staphylococcus aureus, Listeria monocytogenes and Bacillus cereus. 
METHODS USED: Antibacterial activity of clove oil and its dilution was determined by agar well diffusion method and the diameters of clear inhibition zones around the wells were measured. For minimal inhibitory concentration (MIC) is taken the lowest dilution of the clove essential oil that caused a diameter of inhibition zone of 1mm. 
RESULT: The results of the agar well diffusion test showed that the clove essential oil possessed strong antibacterial activity against all bacteria species tested with zones of inhibition ranging from 16 to 23 mm. The slightly higher antibacterial activity was noticed against Gram-negative bacteria (MIC 1,5% v/v) as compared to Gram-positive bacteria (MIC 3% v/v). 
CONCLUSION: Clove essential oil could be a potentially source of novel antibacterial compounds against food-borne pathogenic and spoilage bacteria, alone or in combination with other preservation methods.
PB  - UDRUŽENJE MIKROBIOLOGA SRBIJE
C3  - UDRUŽENJE MIKROBIOLOGA SRBIJE
T1  - Antibacterial activity of clove essential oil against food-borne pathogenic bacteria
SP  - 238
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6120
ER  - 

@conference{
author = "Vukašinović-Sekulić, Maja and Bulatović, Maja and Rakin, Marica and Krunić, Tanja",
year = "2018",
abstract = "INTRODUCTION: Food infection and intoxication are still a major problem worldwide, in spite of the modern methods used to preserve food. Application of synthetic preservatives may lead to negative health consequences and growing public interest is now focussing toward discovery of novel substances from natural sources which will be safer and without any side effects. Among natural antimicrobial agents, spices could be promising alternative to synthetic additives, since at the same time they enhance aroma and flavour and control food-borne pathogens and spoilage bacteria in processed foods. Clove (Syzygium aromaticum) is aromatic spice commonly used in the cuisine of Asian, African, and the Near and Middle East countries. It has many biological properties which mainly derived from high level of eugenol (72-90%) in its essential oil. AIM: The purpose of this study was to evaluate antibacterial activity of commercial clove essential oil against a range of food-born pathogenic bacteria such as Escherichia coli, Salmonella enteritidis, Shigella sonnei, Staphylococcus aureus, Listeria monocytogenes and Bacillus cereus. 
METHODS USED: Antibacterial activity of clove oil and its dilution was determined by agar well diffusion method and the diameters of clear inhibition zones around the wells were measured. For minimal inhibitory concentration (MIC) is taken the lowest dilution of the clove essential oil that caused a diameter of inhibition zone of 1mm. 
RESULT: The results of the agar well diffusion test showed that the clove essential oil possessed strong antibacterial activity against all bacteria species tested with zones of inhibition ranging from 16 to 23 mm. The slightly higher antibacterial activity was noticed against Gram-negative bacteria (MIC 1,5% v/v) as compared to Gram-positive bacteria (MIC 3% v/v). 
CONCLUSION: Clove essential oil could be a potentially source of novel antibacterial compounds against food-borne pathogenic and spoilage bacteria, alone or in combination with other preservation methods.",
publisher = "UDRUŽENJE MIKROBIOLOGA SRBIJE",
journal = "UDRUŽENJE MIKROBIOLOGA SRBIJE",
title = "Antibacterial activity of clove essential oil against food-borne pathogenic bacteria",
pages = "238",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6120"
}

Vukašinović-Sekulić, M., Bulatović, M., Rakin, M.,& Krunić, T.. (2018). Antibacterial activity of clove essential oil against food-borne pathogenic bacteria. in UDRUŽENJE MIKROBIOLOGA SRBIJE
UDRUŽENJE MIKROBIOLOGA SRBIJE., 238.
https://hdl.handle.net/21.15107/rcub_technorep_6120

Vukašinović-Sekulić M, Bulatović M, Rakin M, Krunić T. Antibacterial activity of clove essential oil against food-borne pathogenic bacteria. in UDRUŽENJE MIKROBIOLOGA SRBIJE. 2018;:238.
https://hdl.handle.net/21.15107/rcub_technorep_6120 .

Vukašinović-Sekulić, Maja, Bulatović, Maja, Rakin, Marica, Krunić, Tanja, "Antibacterial activity of clove essential oil against food-borne pathogenic bacteria" in UDRUŽENJE MIKROBIOLOGA SRBIJE (2018):238,
https://hdl.handle.net/21.15107/rcub_technorep_6120 .