TY  - CONF
AU  - Stevanović, Milena
AU  - Đošić, Marija
AU  - Janković, Ana
AU  - Sprecher, Christoph Martin
AU  - Vukašinović-Sekulić, Maja
AU  - Mišković-Stanković, Vesna
PY  - 2024
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7442
AB  - Tissue engineering strives for innovative solutions in addressing challenges associated with contemporary bone tissue implants. This study focuses on the electrophoretic deposition (EPD) of hydroxyapatite-based bioceramic composites containing antibacterial agents onto titanium surfaces. Two composite coatings, hydroxyapa-tite/chitosan (HAP/CS) and hydroxyapatite/chitosan/gentamicin (HAP/CS/Gent) were developed to combat issues such as poor adhesion, limited antibacterial potential, limited bioactivity, and potential toxicity of implant materials [1].
PB  - Savez hemijskih inženjera Srbije
C3  - Hemijska industrija - Supplementary Issue - ExcellMater Conference 2024 Abstracts
T1  - Innovative hydroxyapatite-based coatings for bone implants: A multifaceted approach
IS  - 1S
SP  - 58
VL  - 78
UR  - https://hdl.handle.net/21.15107/rcub_technorep_7442
ER  - 

@conference{
author = "Stevanović, Milena and Đošić, Marija and Janković, Ana and Sprecher, Christoph Martin and Vukašinović-Sekulić, Maja and Mišković-Stanković, Vesna",
year = "2024",
abstract = "Tissue engineering strives for innovative solutions in addressing challenges associated with contemporary bone tissue implants. This study focuses on the electrophoretic deposition (EPD) of hydroxyapatite-based bioceramic composites containing antibacterial agents onto titanium surfaces. Two composite coatings, hydroxyapa-tite/chitosan (HAP/CS) and hydroxyapatite/chitosan/gentamicin (HAP/CS/Gent) were developed to combat issues such as poor adhesion, limited antibacterial potential, limited bioactivity, and potential toxicity of implant materials [1].",
publisher = "Savez hemijskih inženjera Srbije",
journal = "Hemijska industrija - Supplementary Issue - ExcellMater Conference 2024 Abstracts",
title = "Innovative hydroxyapatite-based coatings for bone implants: A multifaceted approach",
number = "1S",
pages = "58",
volume = "78",
url = "https://hdl.handle.net/21.15107/rcub_technorep_7442"
}

Stevanović, M., Đošić, M., Janković, A., Sprecher, C. M., Vukašinović-Sekulić, M.,& Mišković-Stanković, V.. (2024). Innovative hydroxyapatite-based coatings for bone implants: A multifaceted approach. in Hemijska industrija - Supplementary Issue - ExcellMater Conference 2024 Abstracts
Savez hemijskih inženjera Srbije., 78(1S), 58.
https://hdl.handle.net/21.15107/rcub_technorep_7442

Stevanović M, Đošić M, Janković A, Sprecher CM, Vukašinović-Sekulić M, Mišković-Stanković V. Innovative hydroxyapatite-based coatings for bone implants: A multifaceted approach. in Hemijska industrija - Supplementary Issue - ExcellMater Conference 2024 Abstracts. 2024;78(1S):58.
https://hdl.handle.net/21.15107/rcub_technorep_7442 .

Stevanović, Milena, Đošić, Marija, Janković, Ana, Sprecher, Christoph Martin, Vukašinović-Sekulić, Maja, Mišković-Stanković, Vesna, "Innovative hydroxyapatite-based coatings for bone implants: A multifaceted approach" in Hemijska industrija - Supplementary Issue - ExcellMater Conference 2024 Abstracts, 78, no. 1S (2024):58,
https://hdl.handle.net/21.15107/rcub_technorep_7442 .

TY  - JOUR
AU  - Đošić, Marija
AU  - Janković, Ana
AU  - Stevanović, Milena
AU  - Stojanović, Jovica
AU  - Vukašinović-Sekulić, Maja
AU  - Kojić, Vesna
AU  - Mišković-Stanković, Vesna
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6364
AB  - Electrophoretic deposition (EPD) was employed to fabricate composite coatings aimed for orthopedic implants. Hydroxyapatite (HAP), poly(vinyl alcohol) (PVA) and chitosan (CS) coatings, with or without antibiotic gentamicin (Gent) were deposited in a single-step process from aqueous suspension, using EPD. Polymer/mineral composite coatings were formed with the aim to mimic the natural composition of bone tissue, along with improved coatings antibacterial properties, at the same time serving as a drug carrier, allowing local administration of antibiotic. Structural and morphological characterization of HAP/PVA/CS and HAP/PVA/CS/Gent coatings was investigated by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), thermogravimetric analysis (TG/DTG), and scanning electron microscopy (SEM) proving carbonate-substituted HAP. The presence of gentamicin contributed to the bactericidal activity of composite HAP/PVA/CS/Gent coating against Staphylococcus aureus and Escherichia coli. Cytotoxicity studies were performed towards human MRC-5 and mice L929 fibroblast cell lines, and both investigated samples (HAP/PVA/CS and HAP/PVA/CS/Gent) expressed good biocompatibility, without causing toxic effect on tested cells. Biomineralization potential was investigated by measuring the alkaline phosphatase activity, demonstrating high potential of HAP/PVA/CS/Gent to induce the growth of new HAP layer. Therefore, the present investigation proposes the production of composite coatings from non-toxic (aqueous) suspensions, consisting of biocompatible and bioactive components, along with antibiotic gentamicin, improving the antibacterial coatings' properties.
PB  - Elsevier Ltd.
T2  - Materials Chemistry and Physics
T1  - Hydroxyapatite/poly(vinyl alcohol)/chitosan coating with gentamicin for orthopedic implants
SP  - 127766
VL  - 303
DO  - 10.1016/j.matchemphys.2023.127766
ER  - 

@article{
author = "Đošić, Marija and Janković, Ana and Stevanović, Milena and Stojanović, Jovica and Vukašinović-Sekulić, Maja and Kojić, Vesna and Mišković-Stanković, Vesna",
year = "2023",
abstract = "Electrophoretic deposition (EPD) was employed to fabricate composite coatings aimed for orthopedic implants. Hydroxyapatite (HAP), poly(vinyl alcohol) (PVA) and chitosan (CS) coatings, with or without antibiotic gentamicin (Gent) were deposited in a single-step process from aqueous suspension, using EPD. Polymer/mineral composite coatings were formed with the aim to mimic the natural composition of bone tissue, along with improved coatings antibacterial properties, at the same time serving as a drug carrier, allowing local administration of antibiotic. Structural and morphological characterization of HAP/PVA/CS and HAP/PVA/CS/Gent coatings was investigated by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), thermogravimetric analysis (TG/DTG), and scanning electron microscopy (SEM) proving carbonate-substituted HAP. The presence of gentamicin contributed to the bactericidal activity of composite HAP/PVA/CS/Gent coating against Staphylococcus aureus and Escherichia coli. Cytotoxicity studies were performed towards human MRC-5 and mice L929 fibroblast cell lines, and both investigated samples (HAP/PVA/CS and HAP/PVA/CS/Gent) expressed good biocompatibility, without causing toxic effect on tested cells. Biomineralization potential was investigated by measuring the alkaline phosphatase activity, demonstrating high potential of HAP/PVA/CS/Gent to induce the growth of new HAP layer. Therefore, the present investigation proposes the production of composite coatings from non-toxic (aqueous) suspensions, consisting of biocompatible and bioactive components, along with antibiotic gentamicin, improving the antibacterial coatings' properties.",
publisher = "Elsevier Ltd.",
journal = "Materials Chemistry and Physics",
title = "Hydroxyapatite/poly(vinyl alcohol)/chitosan coating with gentamicin for orthopedic implants",
pages = "127766",
volume = "303",
doi = "10.1016/j.matchemphys.2023.127766"
}

Đošić, M., Janković, A., Stevanović, M., Stojanović, J., Vukašinović-Sekulić, M., Kojić, V.,& Mišković-Stanković, V.. (2023). Hydroxyapatite/poly(vinyl alcohol)/chitosan coating with gentamicin for orthopedic implants. in Materials Chemistry and Physics
Elsevier Ltd.., 303, 127766.
https://doi.org/10.1016/j.matchemphys.2023.127766

Đošić M, Janković A, Stevanović M, Stojanović J, Vukašinović-Sekulić M, Kojić V, Mišković-Stanković V. Hydroxyapatite/poly(vinyl alcohol)/chitosan coating with gentamicin for orthopedic implants. in Materials Chemistry and Physics. 2023;303:127766.
doi:10.1016/j.matchemphys.2023.127766 .

Đošić, Marija, Janković, Ana, Stevanović, Milena, Stojanović, Jovica, Vukašinović-Sekulić, Maja, Kojić, Vesna, Mišković-Stanković, Vesna, "Hydroxyapatite/poly(vinyl alcohol)/chitosan coating with gentamicin for orthopedic implants" in Materials Chemistry and Physics, 303 (2023):127766,
https://doi.org/10.1016/j.matchemphys.2023.127766 . .

TY  - JOUR
AU  - Jaćimović, Nevena
AU  - Đošić, Marija
AU  - Janković, Ana
AU  - Grujić, Svetlana
AU  - Matić Bujagić, Ivana
AU  - Stojanović, Jovica
AU  - Vukašinović-Sekulić, Maja
AU  - Kojić, Vesna
AU  - Mišković-Stanković, Vesna
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7260
AB  - Biocomposite hydroxyapatite/poly(vinyl alcohol)/chitosan/gentamicin coatings were fabricated on titanium by an electrophoretic deposition process (EPD) from an aqueous suspension using the constant voltage method. Characterization of the deposited coatings was performed by scanning electron micros-copy with field emission (FE-SEM), Fourier transform infrared spectroscopy (FT-IR), and X-ray diffraction (XRD) after immersion in simulated body fluid (SBF) at 37 °C. The concentration of the released gentamicin was determined using high-performance liquid chromatography (HPLC) coupled with a mass spectrometer (MS). The release profile confirmed an initial “burst-release effect” with ~ 30 % of the gen-tamicin released in the first 48 h, which could be of assistance in the prevention of biofilm formation. An-tibacterial activity was proven by the agar diffusion method on Staphylococcus aureus TL and Escherich-ia coli ATCC 25922 bacterial strains. Cytotoxicity was tested by the dye exclusion test (DET) on MRC-5 and L929 fibroblast cell lines, with both coatings expressing nontoxicity. The results showed the high ap-plicability potential of a poly(vinyl alcohol)-based biocomposite coating for medical purposes.
PB  - Society of Chemists and Technologists of Macedonia
T2  - Macedonian Journal of Chemistry and Chemical Engineering
T1  - Single-step, electrophoretically deposited hydroxyapatite/poly(vinyl alcohol)/chitosan/gentamicin coating for biomedical applications
EP  - 262
IS  - 2
SP  - 249
VL  - 42
DO  - 10.20450/mjcce.2023.2775
ER  - 

@article{
author = "Jaćimović, Nevena and Đošić, Marija and Janković, Ana and Grujić, Svetlana and Matić Bujagić, Ivana and Stojanović, Jovica and Vukašinović-Sekulić, Maja and Kojić, Vesna and Mišković-Stanković, Vesna",
year = "2023",
abstract = "Biocomposite hydroxyapatite/poly(vinyl alcohol)/chitosan/gentamicin coatings were fabricated on titanium by an electrophoretic deposition process (EPD) from an aqueous suspension using the constant voltage method. Characterization of the deposited coatings was performed by scanning electron micros-copy with field emission (FE-SEM), Fourier transform infrared spectroscopy (FT-IR), and X-ray diffraction (XRD) after immersion in simulated body fluid (SBF) at 37 °C. The concentration of the released gentamicin was determined using high-performance liquid chromatography (HPLC) coupled with a mass spectrometer (MS). The release profile confirmed an initial “burst-release effect” with ~ 30 % of the gen-tamicin released in the first 48 h, which could be of assistance in the prevention of biofilm formation. An-tibacterial activity was proven by the agar diffusion method on Staphylococcus aureus TL and Escherich-ia coli ATCC 25922 bacterial strains. Cytotoxicity was tested by the dye exclusion test (DET) on MRC-5 and L929 fibroblast cell lines, with both coatings expressing nontoxicity. The results showed the high ap-plicability potential of a poly(vinyl alcohol)-based biocomposite coating for medical purposes.",
publisher = "Society of Chemists and Technologists of Macedonia",
journal = "Macedonian Journal of Chemistry and Chemical Engineering",
title = "Single-step, electrophoretically deposited hydroxyapatite/poly(vinyl alcohol)/chitosan/gentamicin coating for biomedical applications",
pages = "262-249",
number = "2",
volume = "42",
doi = "10.20450/mjcce.2023.2775"
}

Jaćimović, N., Đošić, M., Janković, A., Grujić, S., Matić Bujagić, I., Stojanović, J., Vukašinović-Sekulić, M., Kojić, V.,& Mišković-Stanković, V.. (2023). Single-step, electrophoretically deposited hydroxyapatite/poly(vinyl alcohol)/chitosan/gentamicin coating for biomedical applications. in Macedonian Journal of Chemistry and Chemical Engineering
Society of Chemists and Technologists of Macedonia., 42(2), 249-262.
https://doi.org/10.20450/mjcce.2023.2775

Jaćimović N, Đošić M, Janković A, Grujić S, Matić Bujagić I, Stojanović J, Vukašinović-Sekulić M, Kojić V, Mišković-Stanković V. Single-step, electrophoretically deposited hydroxyapatite/poly(vinyl alcohol)/chitosan/gentamicin coating for biomedical applications. in Macedonian Journal of Chemistry and Chemical Engineering. 2023;42(2):249-262.
doi:10.20450/mjcce.2023.2775 .

Jaćimović, Nevena, Đošić, Marija, Janković, Ana, Grujić, Svetlana, Matić Bujagić, Ivana, Stojanović, Jovica, Vukašinović-Sekulić, Maja, Kojić, Vesna, Mišković-Stanković, Vesna, "Single-step, electrophoretically deposited hydroxyapatite/poly(vinyl alcohol)/chitosan/gentamicin coating for biomedical applications" in Macedonian Journal of Chemistry and Chemical Engineering, 42, no. 2 (2023):249-262,
https://doi.org/10.20450/mjcce.2023.2775 . .

TY  - JOUR
AU  - Popović, Aleksandra S.
AU  - Gvozdenović, Milica M.
AU  - Janković, Ana
AU  - Jugović, Branimir
AU  - Grgur, Branimir
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6594
AB  - Electrochemical Energy Storage (EES) technologies are playing a significant role in the aspirations to decrease the usage of fossil fuels and move toward an environmentally conscious society. Due to the importance of EES technologies, more researchers are looking for an efficient and effective electrode material, which is the most important part of the EES system that possibly could result in much-needed advancements in the field. However, incoming researchers have a diverse backgrounds and as newcomers to the electrochemical community, they sometimes lack familiarity with the core concepts, well-established procedures, and methodologies that define the standards of the discipline. This issue's importance has been acknowledged, and various publications have been written to guide researchers in doing accurate evaluations. However, to the best of our knowledge, even though these publications demonstrate the methodologies and procedures for approaching the existing challenges none of them address the offered topic with an actual example. To address this gap, we present a step-by-step procedure for the electrochemical analysis of polypyrrole, a widely utilized conducting polymer with significant potential as an electrode material for supercapacitors and batteries.
PB  - Elsevier BV
T2  - Synthetic Metals
T1  - Polypyrrole on graphite: An exemplary model system for comprehensive electrochemical analysis of energy storage materials
SP  - 117386
VL  - 297
DO  - 10.1016/j.synthmet.2023.117386
UR  - https://hdl.handle.net/21.15107/rcub_dais_14707
ER  - 

@article{
author = "Popović, Aleksandra S. and Gvozdenović, Milica M. and Janković, Ana and Jugović, Branimir and Grgur, Branimir",
year = "2023",
abstract = "Electrochemical Energy Storage (EES) technologies are playing a significant role in the aspirations to decrease the usage of fossil fuels and move toward an environmentally conscious society. Due to the importance of EES technologies, more researchers are looking for an efficient and effective electrode material, which is the most important part of the EES system that possibly could result in much-needed advancements in the field. However, incoming researchers have a diverse backgrounds and as newcomers to the electrochemical community, they sometimes lack familiarity with the core concepts, well-established procedures, and methodologies that define the standards of the discipline. This issue's importance has been acknowledged, and various publications have been written to guide researchers in doing accurate evaluations. However, to the best of our knowledge, even though these publications demonstrate the methodologies and procedures for approaching the existing challenges none of them address the offered topic with an actual example. To address this gap, we present a step-by-step procedure for the electrochemical analysis of polypyrrole, a widely utilized conducting polymer with significant potential as an electrode material for supercapacitors and batteries.",
publisher = "Elsevier BV",
journal = "Synthetic Metals",
title = "Polypyrrole on graphite: An exemplary model system for comprehensive electrochemical analysis of energy storage materials",
pages = "117386",
volume = "297",
doi = "10.1016/j.synthmet.2023.117386",
url = "https://hdl.handle.net/21.15107/rcub_dais_14707"
}

Popović, A. S., Gvozdenović, M. M., Janković, A., Jugović, B.,& Grgur, B.. (2023). Polypyrrole on graphite: An exemplary model system for comprehensive electrochemical analysis of energy storage materials. in Synthetic Metals
Elsevier BV., 297, 117386.
https://doi.org/10.1016/j.synthmet.2023.117386
https://hdl.handle.net/21.15107/rcub_dais_14707

Popović AS, Gvozdenović MM, Janković A, Jugović B, Grgur B. Polypyrrole on graphite: An exemplary model system for comprehensive electrochemical analysis of energy storage materials. in Synthetic Metals. 2023;297:117386.
doi:10.1016/j.synthmet.2023.117386
https://hdl.handle.net/21.15107/rcub_dais_14707 .

Popović, Aleksandra S., Gvozdenović, Milica M., Janković, Ana, Jugović, Branimir, Grgur, Branimir, "Polypyrrole on graphite: An exemplary model system for comprehensive electrochemical analysis of energy storage materials" in Synthetic Metals, 297 (2023):117386,
https://doi.org/10.1016/j.synthmet.2023.117386 .,
https://hdl.handle.net/21.15107/rcub_dais_14707 .

TY  - JOUR
AU  - Vanheuverzwijn, Jérome
AU  - Maillard, Eloise-Eliane
AU  - Mahat, Amal
AU  - Fowler, Lee
AU  - Monteyne, Daniel
AU  - Bonnaud, Leïla
AU  - Landercy, Nicolas
AU  - Hemberg, Axel
AU  - Janković, Ana
AU  - Meyer, Franck
AU  - Mišković-Stanković, Vesna
AU  - Stevanović, Milena
AU  - Mirica, Codruta
AU  - Pérez-Morga, David
AU  - Luginbuehl, Reto
AU  - Combes, Christèle
AU  - Furtos, Gabriel
AU  - Fontaine, Véronique
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6381
AB  - Medical implants have improved the quality of life of many patients. However, surgical intervention may eventually lead to implant microbial contamination. The aims of this research were to develop an easy, robust, quantitative assay to assess surface antimicrobial activities, especially the anti-nascent biofilm activity, and to identify control surfaces, allowing for international comparisons. Using new antimicrobial assays to assess the inhibition of nascent biofilm during persistent contact or after transient contact with bacteria, we show that the 5 cent Euro coin or other metal-based antibacterial coins can be used as positive controls, as more than 4 log reduction on bacterial survival was observed when using either S. aureus or P. aeruginosa as targets. The methods and controls described here could be useful to develop an easy, flexible and standardizable assay to assess relevant antimicrobial activities of new implant materials developed by industries and academics.
PB  - MDPI
T2  - Microorganisms
T1  - Easy, Flexible and Standardizable Anti-Nascent Biofilm Activity Assay to Assess Implant Materials
IS  - 4
SP  - 1023
VL  - 11
DO  - 10.3390/microorganisms11041023
ER  - 

@article{
author = "Vanheuverzwijn, Jérome and Maillard, Eloise-Eliane and Mahat, Amal and Fowler, Lee and Monteyne, Daniel and Bonnaud, Leïla and Landercy, Nicolas and Hemberg, Axel and Janković, Ana and Meyer, Franck and Mišković-Stanković, Vesna and Stevanović, Milena and Mirica, Codruta and Pérez-Morga, David and Luginbuehl, Reto and Combes, Christèle and Furtos, Gabriel and Fontaine, Véronique",
year = "2023",
abstract = "Medical implants have improved the quality of life of many patients. However, surgical intervention may eventually lead to implant microbial contamination. The aims of this research were to develop an easy, robust, quantitative assay to assess surface antimicrobial activities, especially the anti-nascent biofilm activity, and to identify control surfaces, allowing for international comparisons. Using new antimicrobial assays to assess the inhibition of nascent biofilm during persistent contact or after transient contact with bacteria, we show that the 5 cent Euro coin or other metal-based antibacterial coins can be used as positive controls, as more than 4 log reduction on bacterial survival was observed when using either S. aureus or P. aeruginosa as targets. The methods and controls described here could be useful to develop an easy, flexible and standardizable assay to assess relevant antimicrobial activities of new implant materials developed by industries and academics.",
publisher = "MDPI",
journal = "Microorganisms",
title = "Easy, Flexible and Standardizable Anti-Nascent Biofilm Activity Assay to Assess Implant Materials",
number = "4",
pages = "1023",
volume = "11",
doi = "10.3390/microorganisms11041023"
}

Vanheuverzwijn, J., Maillard, E., Mahat, A., Fowler, L., Monteyne, D., Bonnaud, L., Landercy, N., Hemberg, A., Janković, A., Meyer, F., Mišković-Stanković, V., Stevanović, M., Mirica, C., Pérez-Morga, D., Luginbuehl, R., Combes, C., Furtos, G.,& Fontaine, V.. (2023). Easy, Flexible and Standardizable Anti-Nascent Biofilm Activity Assay to Assess Implant Materials. in Microorganisms
MDPI., 11(4), 1023.
https://doi.org/10.3390/microorganisms11041023

Vanheuverzwijn J, Maillard E, Mahat A, Fowler L, Monteyne D, Bonnaud L, Landercy N, Hemberg A, Janković A, Meyer F, Mišković-Stanković V, Stevanović M, Mirica C, Pérez-Morga D, Luginbuehl R, Combes C, Furtos G, Fontaine V. Easy, Flexible and Standardizable Anti-Nascent Biofilm Activity Assay to Assess Implant Materials. in Microorganisms. 2023;11(4):1023.
doi:10.3390/microorganisms11041023 .

Vanheuverzwijn, Jérome, Maillard, Eloise-Eliane, Mahat, Amal, Fowler, Lee, Monteyne, Daniel, Bonnaud, Leïla, Landercy, Nicolas, Hemberg, Axel, Janković, Ana, Meyer, Franck, Mišković-Stanković, Vesna, Stevanović, Milena, Mirica, Codruta, Pérez-Morga, David, Luginbuehl, Reto, Combes, Christèle, Furtos, Gabriel, Fontaine, Véronique, "Easy, Flexible and Standardizable Anti-Nascent Biofilm Activity Assay to Assess Implant Materials" in Microorganisms, 11, no. 4 (2023):1023,
https://doi.org/10.3390/microorganisms11041023 . .

TY  - CONF
AU  - Nešović, Katarina
AU  - Janković, Ana
AU  - Surudžić, Rade
AU  - Vukašinović-Sekulić, Maja
AU  - Kojić, Vesna
AU  - Radetić, Tamara
AU  - Mišković-Stanković, Vesna
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6761
AB  - Recent advances in biomaterials science are increasingly focused
on (re)search for next-generation soft tissue regeneration solutions.
Hydrogel-based materials are becoming a focal point to address
many tissue engineering requirements, possessing exceptional biocompatibility,
with tailorable shape, structure and mechanical
properties, as well as infinite possibilities drug delivery. The widespread
antibiotics use has led to aggravated resistance issues in many
bacterial strains, so the focus is increasingly shifted towards alternative
antibacterial solutions such as silver nanoparticles (AgNPs).
In this work, we have developed novel composite materials based on
poly(vinyl alcohol)/chitosan (PVA/CHI) and poly(vinyl alcohol)/
chitosan/graphene (PVA/CHI/Gr) hydrogels with silver nanoparticles
obtained through green in situ constant-voltage electrochemical
synthesis. The obtained AgNPs’ properties, sizes and size
distributions were examined using UV-visible spectroscopy, dynamic
light scattering and transmission electron microscopy. In vitro
swelling and silver release kinetics were monitored in the simulated
physiological conditions (pH7.4, 37  C). The obtained release and
swelling isotherms were fitted with several theoretical models that
helped discern the mechanisms of these processes. Tensile testing
was carried out to examine the composites’ mechanical behavior and
elasticity – important properties for soft tissue engineering materials.
Finally, the excellent antibacterial activity of the obtained silver/
poly(vinyl alcohol)/chitosan (Ag/PVA/CHI) and silver/poly(vinyl
alcohol)/chitosan/graphene (Ag/PVA/CHI/Gr) hydrogels against
Staphylococcus aureus and Escherichia coli was confirmed quantitatively
by colony counting method, and their non-toxicity was
verified towards two model fibroblast cell lines (MRC-5 and L929)
utilizing the MTT assay. Through thorough characterization, the
obtained nanocomposite hydrogel materials were identified as strong
candidates for active antibacterial soft tissue engineering materials.
PB  - Mary Ann Liebert, Inc.
C3  - Tissue Engineering Part A
T1  - Silver Nanoparticles-Loaded Poly(Vinyl Alcohol)/Chitosan/Graphene Hydrogels Obtained by Electrochemical Synthesis
IS  - S1
SP  - S-132
VL  - 28
DO  - 10.1089/ten.tea.2022.29025.abstracts
ER  - 

@conference{
author = "Nešović, Katarina and Janković, Ana and Surudžić, Rade and Vukašinović-Sekulić, Maja and Kojić, Vesna and Radetić, Tamara and Mišković-Stanković, Vesna",
year = "2022",
abstract = "Recent advances in biomaterials science are increasingly focused
on (re)search for next-generation soft tissue regeneration solutions.
Hydrogel-based materials are becoming a focal point to address
many tissue engineering requirements, possessing exceptional biocompatibility,
with tailorable shape, structure and mechanical
properties, as well as infinite possibilities drug delivery. The widespread
antibiotics use has led to aggravated resistance issues in many
bacterial strains, so the focus is increasingly shifted towards alternative
antibacterial solutions such as silver nanoparticles (AgNPs).
In this work, we have developed novel composite materials based on
poly(vinyl alcohol)/chitosan (PVA/CHI) and poly(vinyl alcohol)/
chitosan/graphene (PVA/CHI/Gr) hydrogels with silver nanoparticles
obtained through green in situ constant-voltage electrochemical
synthesis. The obtained AgNPs’ properties, sizes and size
distributions were examined using UV-visible spectroscopy, dynamic
light scattering and transmission electron microscopy. In vitro
swelling and silver release kinetics were monitored in the simulated
physiological conditions (pH7.4, 37  C). The obtained release and
swelling isotherms were fitted with several theoretical models that
helped discern the mechanisms of these processes. Tensile testing
was carried out to examine the composites’ mechanical behavior and
elasticity – important properties for soft tissue engineering materials.
Finally, the excellent antibacterial activity of the obtained silver/
poly(vinyl alcohol)/chitosan (Ag/PVA/CHI) and silver/poly(vinyl
alcohol)/chitosan/graphene (Ag/PVA/CHI/Gr) hydrogels against
Staphylococcus aureus and Escherichia coli was confirmed quantitatively
by colony counting method, and their non-toxicity was
verified towards two model fibroblast cell lines (MRC-5 and L929)
utilizing the MTT assay. Through thorough characterization, the
obtained nanocomposite hydrogel materials were identified as strong
candidates for active antibacterial soft tissue engineering materials.",
publisher = "Mary Ann Liebert, Inc.",
journal = "Tissue Engineering Part A",
title = "Silver Nanoparticles-Loaded Poly(Vinyl Alcohol)/Chitosan/Graphene Hydrogels Obtained by Electrochemical Synthesis",
number = "S1",
pages = "S-132",
volume = "28",
doi = "10.1089/ten.tea.2022.29025.abstracts"
}

Nešović, K., Janković, A., Surudžić, R., Vukašinović-Sekulić, M., Kojić, V., Radetić, T.,& Mišković-Stanković, V.. (2022). Silver Nanoparticles-Loaded Poly(Vinyl Alcohol)/Chitosan/Graphene Hydrogels Obtained by Electrochemical Synthesis. in Tissue Engineering Part A
Mary Ann Liebert, Inc.., 28(S1), S-132.
https://doi.org/10.1089/ten.tea.2022.29025.abstracts

Nešović K, Janković A, Surudžić R, Vukašinović-Sekulić M, Kojić V, Radetić T, Mišković-Stanković V. Silver Nanoparticles-Loaded Poly(Vinyl Alcohol)/Chitosan/Graphene Hydrogels Obtained by Electrochemical Synthesis. in Tissue Engineering Part A. 2022;28(S1):S-132.
doi:10.1089/ten.tea.2022.29025.abstracts .

Nešović, Katarina, Janković, Ana, Surudžić, Rade, Vukašinović-Sekulić, Maja, Kojić, Vesna, Radetić, Tamara, Mišković-Stanković, Vesna, "Silver Nanoparticles-Loaded Poly(Vinyl Alcohol)/Chitosan/Graphene Hydrogels Obtained by Electrochemical Synthesis" in Tissue Engineering Part A, 28, no. S1 (2022):S-132,
https://doi.org/10.1089/ten.tea.2022.29025.abstracts . .

TY  - JOUR
AU  - Stevanović, Milena
AU  - Đošić, Marija
AU  - Janković, Ana
AU  - Kojić, Vesna
AU  - Stojanović, Jovica
AU  - Grujić, Svetlana
AU  - Matić-Bujagić, Ivana
AU  - Rhee, Kyong Yop
AU  - Mišković-Stanković, Vesna
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4839
AB  - This paper describes a promising electrophoretic deposition (EPD) approach for developing composite coatings based on chitosan with the additional components of hydroxyapatite, graphene, and gentamicin on titanium substrate. Bioactive properties were investigated in vitro by immersing the coatings in simulated body fluid (SBF) at 37 degrees C. The newly formed biomimetic layer on the top of the deposited chitosan-based coatings on Ti was confirmed by X-ray diffraction, Fourier transform infrared spectroscopy, field emission scanning electron microscopy and electrochemical measurements, while coatings' bioactivity was proved by alkaline phosphatase activity assay in MRC-5 and L929 tested cell lines. The biocompatibility towards MRC-5 and L929 cell lines was investigated by dye exclusion test (DET) implying the non-cytotoxic effect of coatings. Gentamicin release studies were monitored during 21-day by high-performance liquid chromatography coupled with mass spectrometry, indicating rapid release of gentamicin (approximate to 40%) in the first 48 h and more than 60% after 14 days.
PB  - Elsevier B.V.
T2  - Journal of Materials Research and Technology
T1  - The chitosan-based bioactive composite coating on titanium
EP  - 4474
SP  - 4461
VL  - 15
DO  - 10.1016/j.jmrt.2021.10.072
ER  - 

@article{
author = "Stevanović, Milena and Đošić, Marija and Janković, Ana and Kojić, Vesna and Stojanović, Jovica and Grujić, Svetlana and Matić-Bujagić, Ivana and Rhee, Kyong Yop and Mišković-Stanković, Vesna",
year = "2021",
abstract = "This paper describes a promising electrophoretic deposition (EPD) approach for developing composite coatings based on chitosan with the additional components of hydroxyapatite, graphene, and gentamicin on titanium substrate. Bioactive properties were investigated in vitro by immersing the coatings in simulated body fluid (SBF) at 37 degrees C. The newly formed biomimetic layer on the top of the deposited chitosan-based coatings on Ti was confirmed by X-ray diffraction, Fourier transform infrared spectroscopy, field emission scanning electron microscopy and electrochemical measurements, while coatings' bioactivity was proved by alkaline phosphatase activity assay in MRC-5 and L929 tested cell lines. The biocompatibility towards MRC-5 and L929 cell lines was investigated by dye exclusion test (DET) implying the non-cytotoxic effect of coatings. Gentamicin release studies were monitored during 21-day by high-performance liquid chromatography coupled with mass spectrometry, indicating rapid release of gentamicin (approximate to 40%) in the first 48 h and more than 60% after 14 days.",
publisher = "Elsevier B.V.",
journal = "Journal of Materials Research and Technology",
title = "The chitosan-based bioactive composite coating on titanium",
pages = "4474-4461",
volume = "15",
doi = "10.1016/j.jmrt.2021.10.072"
}

Stevanović, M., Đošić, M., Janković, A., Kojić, V., Stojanović, J., Grujić, S., Matić-Bujagić, I., Rhee, K. Y.,& Mišković-Stanković, V.. (2021). The chitosan-based bioactive composite coating on titanium. in Journal of Materials Research and Technology
Elsevier B.V.., 15, 4461-4474.
https://doi.org/10.1016/j.jmrt.2021.10.072

Stevanović M, Đošić M, Janković A, Kojić V, Stojanović J, Grujić S, Matić-Bujagić I, Rhee KY, Mišković-Stanković V. The chitosan-based bioactive composite coating on titanium. in Journal of Materials Research and Technology. 2021;15:4461-4474.
doi:10.1016/j.jmrt.2021.10.072 .

Stevanović, Milena, Đošić, Marija, Janković, Ana, Kojić, Vesna, Stojanović, Jovica, Grujić, Svetlana, Matić-Bujagić, Ivana, Rhee, Kyong Yop, Mišković-Stanković, Vesna, "The chitosan-based bioactive composite coating on titanium" in Journal of Materials Research and Technology, 15 (2021):4461-4474,
https://doi.org/10.1016/j.jmrt.2021.10.072 . .

TY  - JOUR
AU  - Djosic, Marija
AU  - Janković, Ana
AU  - Mišković-Stanković, Vesna
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4792
AB  - Current trends in biomaterials science address the issue of integrating artificial materials as orthopedic or dental implants with biological materials, e.g., patients' bone tissue. Problems arise due to the simple fact that any surface that promotes biointegration and facilitates osteointegration may also provide a good platform for the rapid growth of bacterial colonies. Infected implant surfaces easily lead to biofilm formation that poses a major healthcare concern since it could have destructive effects and ultimately endanger the patients' life. As of late, research has centered on designing coatings that would eliminate possible infection but neglected to aid bone mineralization. Other strategies yielded surfaces that could promote osseointegration but failed to prevent microbial susceptibility. Needless to say, in order to assure prolonged implant functionality, both coating functions are indispensable and should be addressed simultaneously. This review summarizes progress in designing multifunctional implant coatings that serve as carriers of antibacterial agents with the primary intention of inhibiting bacterial growth on the implant-tissue interface, while still promoting osseointegration.
T2  - Materials
T1  - Electrophoretic Deposition of Biocompatible and Bioactive Hydroxyapatite-Based Coatings on Titanium
IS  - 18
VL  - 14
DO  - 10.3390/ma14185391
ER  - 

@article{
author = "Djosic, Marija and Janković, Ana and Mišković-Stanković, Vesna",
year = "2021",
abstract = "Current trends in biomaterials science address the issue of integrating artificial materials as orthopedic or dental implants with biological materials, e.g., patients' bone tissue. Problems arise due to the simple fact that any surface that promotes biointegration and facilitates osteointegration may also provide a good platform for the rapid growth of bacterial colonies. Infected implant surfaces easily lead to biofilm formation that poses a major healthcare concern since it could have destructive effects and ultimately endanger the patients' life. As of late, research has centered on designing coatings that would eliminate possible infection but neglected to aid bone mineralization. Other strategies yielded surfaces that could promote osseointegration but failed to prevent microbial susceptibility. Needless to say, in order to assure prolonged implant functionality, both coating functions are indispensable and should be addressed simultaneously. This review summarizes progress in designing multifunctional implant coatings that serve as carriers of antibacterial agents with the primary intention of inhibiting bacterial growth on the implant-tissue interface, while still promoting osseointegration.",
journal = "Materials",
title = "Electrophoretic Deposition of Biocompatible and Bioactive Hydroxyapatite-Based Coatings on Titanium",
number = "18",
volume = "14",
doi = "10.3390/ma14185391"
}

Djosic, M., Janković, A.,& Mišković-Stanković, V.. (2021). Electrophoretic Deposition of Biocompatible and Bioactive Hydroxyapatite-Based Coatings on Titanium. in Materials, 14(18).
https://doi.org/10.3390/ma14185391

Djosic M, Janković A, Mišković-Stanković V. Electrophoretic Deposition of Biocompatible and Bioactive Hydroxyapatite-Based Coatings on Titanium. in Materials. 2021;14(18).
doi:10.3390/ma14185391 .

Djosic, Marija, Janković, Ana, Mišković-Stanković, Vesna, "Electrophoretic Deposition of Biocompatible and Bioactive Hydroxyapatite-Based Coatings on Titanium" in Materials, 14, no. 18 (2021),
https://doi.org/10.3390/ma14185391 . .

TY  - CONF
AU  - Jaćimović, Nevena
AU  - Đošić, Marija
AU  - Janković, Ana
AU  - Mišković-Stanković, Vesna
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5029
AB  - Composite bioceramic coatings have been attracting increasing attention of the scientific
public in recent decades due to the good potential of their use for medical purposes. The
main goal of this scientific research is to show the characterization of the obtained
hydroxyapatite (HAP), chitosan (CS) and polyvinyl alcohol (PVA) bioceramic composite
coatings loaded with gentamicin (Gent), on titanium (Ti) substrate using the single-step
electrophoretic deposition process. Titanium, traditionally used for the purpose of replacing
parts of damaged bone tissue, has shown good mechanical properties and biocompatibility
through many years of orthopedic practice. Hydroxyapatite provided the HAP/CS/PVA/Gent
composite coating with osteoinductivity, while the presence of chitosan and polyvinyl
alcohol contributed to its adhesive and mild analgesic properties. The antibacterial agent
gentamicin was added to the HAP/CS/PVA composite, which ensured its direct antibacterial
effect at the implantation site. The coatings were deposited using the cathodic electrophoretic
deposition process, with the Ti plate as the cathode, at a constant voltage of 7V, in the range
of the deposition time from 5 to 12 min. To demonstrate the presumed bioactivity,
HAP/CS/PVA/Gent coatings were immersed in simulated body fluid (SBF) at 37⁰C for
periods of 7 and 14 days. Characterization of HAP/CS/PVA/Gent coatings after immersion
was performed through employment of Fourier transform infrared spectroscopy (FTIR), X ray diffraction (XRD), and field emission scanning electron microscopy (SEM) techniques.
Antibacterial activity was tested by agar diffusion method on two bacterial strains,
Echerichia coli and Staphylococcus aureus with a positive response to both bacterial strains.
The final conclusions of this research suggest a good potential for utilization of
HAP/CS/PVA/Gent bioceramic composite coating, applied through electrophoretic
deposition process on Ti substrate, for medical purposes in the replacement and healing of
damaged bone tissue.
PB  - Institute of Technical Sciences of SASA
C3  - Nineteenth Young Researchers’ Conference - Materials Science and Engineering: Program and the Book of Abstracts
T1  - Electrochemical composite bioceramic coatings based on hydroxyapatite, chitosan and polyvinyl alcohol loaded with gentamicin
SP  - 7
VL  - 18
UR  - https://hdl.handle.net/21.15107/rcub_technorep_5029
ER  - 

@conference{
author = "Jaćimović, Nevena and Đošić, Marija and Janković, Ana and Mišković-Stanković, Vesna",
year = "2021",
abstract = "Composite bioceramic coatings have been attracting increasing attention of the scientific
public in recent decades due to the good potential of their use for medical purposes. The
main goal of this scientific research is to show the characterization of the obtained
hydroxyapatite (HAP), chitosan (CS) and polyvinyl alcohol (PVA) bioceramic composite
coatings loaded with gentamicin (Gent), on titanium (Ti) substrate using the single-step
electrophoretic deposition process. Titanium, traditionally used for the purpose of replacing
parts of damaged bone tissue, has shown good mechanical properties and biocompatibility
through many years of orthopedic practice. Hydroxyapatite provided the HAP/CS/PVA/Gent
composite coating with osteoinductivity, while the presence of chitosan and polyvinyl
alcohol contributed to its adhesive and mild analgesic properties. The antibacterial agent
gentamicin was added to the HAP/CS/PVA composite, which ensured its direct antibacterial
effect at the implantation site. The coatings were deposited using the cathodic electrophoretic
deposition process, with the Ti plate as the cathode, at a constant voltage of 7V, in the range
of the deposition time from 5 to 12 min. To demonstrate the presumed bioactivity,
HAP/CS/PVA/Gent coatings were immersed in simulated body fluid (SBF) at 37⁰C for
periods of 7 and 14 days. Characterization of HAP/CS/PVA/Gent coatings after immersion
was performed through employment of Fourier transform infrared spectroscopy (FTIR), X ray diffraction (XRD), and field emission scanning electron microscopy (SEM) techniques.
Antibacterial activity was tested by agar diffusion method on two bacterial strains,
Echerichia coli and Staphylococcus aureus with a positive response to both bacterial strains.
The final conclusions of this research suggest a good potential for utilization of
HAP/CS/PVA/Gent bioceramic composite coating, applied through electrophoretic
deposition process on Ti substrate, for medical purposes in the replacement and healing of
damaged bone tissue.",
publisher = "Institute of Technical Sciences of SASA",
journal = "Nineteenth Young Researchers’ Conference - Materials Science and Engineering: Program and the Book of Abstracts",
title = "Electrochemical composite bioceramic coatings based on hydroxyapatite, chitosan and polyvinyl alcohol loaded with gentamicin",
pages = "7",
volume = "18",
url = "https://hdl.handle.net/21.15107/rcub_technorep_5029"
}

Jaćimović, N., Đošić, M., Janković, A.,& Mišković-Stanković, V.. (2021). Electrochemical composite bioceramic coatings based on hydroxyapatite, chitosan and polyvinyl alcohol loaded with gentamicin. in Nineteenth Young Researchers’ Conference - Materials Science and Engineering: Program and the Book of Abstracts
Institute of Technical Sciences of SASA., 18, 7.
https://hdl.handle.net/21.15107/rcub_technorep_5029

Jaćimović N, Đošić M, Janković A, Mišković-Stanković V. Electrochemical composite bioceramic coatings based on hydroxyapatite, chitosan and polyvinyl alcohol loaded with gentamicin. in Nineteenth Young Researchers’ Conference - Materials Science and Engineering: Program and the Book of Abstracts. 2021;18:7.
https://hdl.handle.net/21.15107/rcub_technorep_5029 .

Jaćimović, Nevena, Đošić, Marija, Janković, Ana, Mišković-Stanković, Vesna, "Electrochemical composite bioceramic coatings based on hydroxyapatite, chitosan and polyvinyl alcohol loaded with gentamicin" in Nineteenth Young Researchers’ Conference - Materials Science and Engineering: Program and the Book of Abstracts, 18 (2021):7,
https://hdl.handle.net/21.15107/rcub_technorep_5029 .

TY  - JOUR
AU  - Nešović, Katarina
AU  - Janković, Ana
AU  - Radetić, Tamara
AU  - Perić-Grujić, Aleksandra
AU  - Vukašinović-Sekulić, Maja
AU  - Kojić, Vesna
AU  - Rhee, Kyong Yop
AU  - Mišković-Stanković, Vesna
PY  - 2020
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4495
AB  - Polymer-based hydrogel materials are excellent candidates for new-generation wound dressings with improved properties, such as high sorption ability, good mechanical properties and low adhesiveness. Cross-linked hydrogel matrices also serve as excellent carriers for controlled release of antibacterial agents, such as silver nanoparticles (AgNPs), which are preferred over conventional antibiotics due to low propensity to induce bacterial resistance. In this work, we aim to produce novel silver/poly(vinyl alcohol)/chitosan (Ag/PVA/CHI) hydrogels for wound dressing applications. The electrochemical AgNPs synthesis provided facile and green method for the reduction of Ag+ ions inside the hydrogel matrices, without the need to use toxic chemical reducing agents. The formation of AgNPs was confirmed using UV-visible spectroscopy, scanning and transmission electron microscopy. Release kinetics was investigated in modified phosphate buffer solution at 37 degrees C to mimic physiological conditions. Release profiles indicated "burst release" behavior, which is beneficial for wound dressing applications. The antibacterial activity was evaluated against Staphylococcus aureus and Escherichia coli strains using disc-diffusion test, and non-toxicity of hydrogels was proved by dye-exclusion test. The obtained results confirmed strong potential of Ag/PVA/CHI hydrogels for biomedical applications.
PB  - Int Assoc Physical Chemists-Iapc, Zagreb
T2  - Journal of Electrochemical Science and Engineering
T1  - Poly(vinyl alcohol)/chitosan hydrogels with electrochemically synthesized silver nanoparticles for wound dressing applications
EP  - 198
IS  - 2
SP  - 185
VL  - 10
DO  - 10.5599/jese.732
ER  - 

@article{
author = "Nešović, Katarina and Janković, Ana and Radetić, Tamara and Perić-Grujić, Aleksandra and Vukašinović-Sekulić, Maja and Kojić, Vesna and Rhee, Kyong Yop and Mišković-Stanković, Vesna",
year = "2020",
abstract = "Polymer-based hydrogel materials are excellent candidates for new-generation wound dressings with improved properties, such as high sorption ability, good mechanical properties and low adhesiveness. Cross-linked hydrogel matrices also serve as excellent carriers for controlled release of antibacterial agents, such as silver nanoparticles (AgNPs), which are preferred over conventional antibiotics due to low propensity to induce bacterial resistance. In this work, we aim to produce novel silver/poly(vinyl alcohol)/chitosan (Ag/PVA/CHI) hydrogels for wound dressing applications. The electrochemical AgNPs synthesis provided facile and green method for the reduction of Ag+ ions inside the hydrogel matrices, without the need to use toxic chemical reducing agents. The formation of AgNPs was confirmed using UV-visible spectroscopy, scanning and transmission electron microscopy. Release kinetics was investigated in modified phosphate buffer solution at 37 degrees C to mimic physiological conditions. Release profiles indicated "burst release" behavior, which is beneficial for wound dressing applications. The antibacterial activity was evaluated against Staphylococcus aureus and Escherichia coli strains using disc-diffusion test, and non-toxicity of hydrogels was proved by dye-exclusion test. The obtained results confirmed strong potential of Ag/PVA/CHI hydrogels for biomedical applications.",
publisher = "Int Assoc Physical Chemists-Iapc, Zagreb",
journal = "Journal of Electrochemical Science and Engineering",
title = "Poly(vinyl alcohol)/chitosan hydrogels with electrochemically synthesized silver nanoparticles for wound dressing applications",
pages = "198-185",
number = "2",
volume = "10",
doi = "10.5599/jese.732"
}

Nešović, K., Janković, A., Radetić, T., Perić-Grujić, A., Vukašinović-Sekulić, M., Kojić, V., Rhee, K. Y.,& Mišković-Stanković, V.. (2020). Poly(vinyl alcohol)/chitosan hydrogels with electrochemically synthesized silver nanoparticles for wound dressing applications. in Journal of Electrochemical Science and Engineering
Int Assoc Physical Chemists-Iapc, Zagreb., 10(2), 185-198.
https://doi.org/10.5599/jese.732

Nešović K, Janković A, Radetić T, Perić-Grujić A, Vukašinović-Sekulić M, Kojić V, Rhee KY, Mišković-Stanković V. Poly(vinyl alcohol)/chitosan hydrogels with electrochemically synthesized silver nanoparticles for wound dressing applications. in Journal of Electrochemical Science and Engineering. 2020;10(2):185-198.
doi:10.5599/jese.732 .

Nešović, Katarina, Janković, Ana, Radetić, Tamara, Perić-Grujić, Aleksandra, Vukašinović-Sekulić, Maja, Kojić, Vesna, Rhee, Kyong Yop, Mišković-Stanković, Vesna, "Poly(vinyl alcohol)/chitosan hydrogels with electrochemically synthesized silver nanoparticles for wound dressing applications" in Journal of Electrochemical Science and Engineering, 10, no. 2 (2020):185-198,
https://doi.org/10.5599/jese.732 . .

TY  - JOUR
AU  - Stevanović, Milena
AU  - Đošić, Marija
AU  - Janković, Ana
AU  - Kojić, Vesna
AU  - Vukašinović-Sekulić, Maja
AU  - Stojanović, Jovica
AU  - Odović, Jadranka
AU  - Crevar-Sakač, Milkica
AU  - Kyong Yop, Rhee
AU  - Mišković-Stanković, Vesna
PY  - 2020
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4501
AB  - Electrophoretic deposition process (EPD) was successfully used for obtaining graphene (Gr)-reinforced composite coating based on hydroxyapatite (HAP), chitosan (CS), and antibiotic gentamicin (Gent), from aqueous suspension. The deposition process was performed as a single step process at a constant voltage (5 V, deposition time 12 min) on pure titanium foils. The influence of graphene was examined through detailed physicochemical and biological characterization. Fourier transform infrared spectroscopy, field emission scanning electron microscopy, thermogravimetric analysis, X-ray diffraction, Raman, and X-ray photoelectron analyses confirmed the formation of composite HAP/CS/Gr and HAP/CS/Gr/Gent coatings on Ti. Obtained coatings had porous, uniform, fracture-free surfaces, suggesting strong interfacial interaction between HAP, CS, and Gr. Large specific area of graphene enabled strong bonding with chitosan, acting as nanofiller throughout the polymer matrix. Gentamicin addition strongly improved the antibacterial activity of HAP/CS/Gr/Gent coating that was confirmed by antibacterial activity kinetics in suspension and agar diffusion testing, while results indicated more pronounced antibacterial effect againstStaphylococcus aureus(bactericidal, viable cells number reduction  gt 3 logarithmic units) compared toEscherichia coli(bacteriostatic, lt 3 logarithmic units).MTT assay indicated low cytotoxicity (75% cell viability) against MRC-5 and L929 (70% cell viability) tested cell lines, indicating good biocompatibility of HAP/CS/Gr/Gent coating. Therefore, electrodeposited HAP/CS/Gr/Gent coating on Ti can be considered as a prospective material for bone tissue engineering as a hard tissue implant.
PB  - Wiley, Hoboken
T2  - Journal of Biomedical Materials Research Part A
T1  - Antibacterial graphene-based hydroxyapatite/chitosan coating with gentamicin for potential applications in bone tissue engineering
EP  - 2189
IS  - 11
SP  - 2175
VL  - 108
DO  - 10.1002/jbm.a.36974
ER  - 

@article{
author = "Stevanović, Milena and Đošić, Marija and Janković, Ana and Kojić, Vesna and Vukašinović-Sekulić, Maja and Stojanović, Jovica and Odović, Jadranka and Crevar-Sakač, Milkica and Kyong Yop, Rhee and Mišković-Stanković, Vesna",
year = "2020",
abstract = "Electrophoretic deposition process (EPD) was successfully used for obtaining graphene (Gr)-reinforced composite coating based on hydroxyapatite (HAP), chitosan (CS), and antibiotic gentamicin (Gent), from aqueous suspension. The deposition process was performed as a single step process at a constant voltage (5 V, deposition time 12 min) on pure titanium foils. The influence of graphene was examined through detailed physicochemical and biological characterization. Fourier transform infrared spectroscopy, field emission scanning electron microscopy, thermogravimetric analysis, X-ray diffraction, Raman, and X-ray photoelectron analyses confirmed the formation of composite HAP/CS/Gr and HAP/CS/Gr/Gent coatings on Ti. Obtained coatings had porous, uniform, fracture-free surfaces, suggesting strong interfacial interaction between HAP, CS, and Gr. Large specific area of graphene enabled strong bonding with chitosan, acting as nanofiller throughout the polymer matrix. Gentamicin addition strongly improved the antibacterial activity of HAP/CS/Gr/Gent coating that was confirmed by antibacterial activity kinetics in suspension and agar diffusion testing, while results indicated more pronounced antibacterial effect againstStaphylococcus aureus(bactericidal, viable cells number reduction  gt 3 logarithmic units) compared toEscherichia coli(bacteriostatic, lt 3 logarithmic units).MTT assay indicated low cytotoxicity (75% cell viability) against MRC-5 and L929 (70% cell viability) tested cell lines, indicating good biocompatibility of HAP/CS/Gr/Gent coating. Therefore, electrodeposited HAP/CS/Gr/Gent coating on Ti can be considered as a prospective material for bone tissue engineering as a hard tissue implant.",
publisher = "Wiley, Hoboken",
journal = "Journal of Biomedical Materials Research Part A",
title = "Antibacterial graphene-based hydroxyapatite/chitosan coating with gentamicin for potential applications in bone tissue engineering",
pages = "2189-2175",
number = "11",
volume = "108",
doi = "10.1002/jbm.a.36974"
}

Stevanović, M., Đošić, M., Janković, A., Kojić, V., Vukašinović-Sekulić, M., Stojanović, J., Odović, J., Crevar-Sakač, M., Kyong Yop, R.,& Mišković-Stanković, V.. (2020). Antibacterial graphene-based hydroxyapatite/chitosan coating with gentamicin for potential applications in bone tissue engineering. in Journal of Biomedical Materials Research Part A
Wiley, Hoboken., 108(11), 2175-2189.
https://doi.org/10.1002/jbm.a.36974

Stevanović M, Đošić M, Janković A, Kojić V, Vukašinović-Sekulić M, Stojanović J, Odović J, Crevar-Sakač M, Kyong Yop R, Mišković-Stanković V. Antibacterial graphene-based hydroxyapatite/chitosan coating with gentamicin for potential applications in bone tissue engineering. in Journal of Biomedical Materials Research Part A. 2020;108(11):2175-2189.
doi:10.1002/jbm.a.36974 .

Stevanović, Milena, Đošić, Marija, Janković, Ana, Kojić, Vesna, Vukašinović-Sekulić, Maja, Stojanović, Jovica, Odović, Jadranka, Crevar-Sakač, Milkica, Kyong Yop, Rhee, Mišković-Stanković, Vesna, "Antibacterial graphene-based hydroxyapatite/chitosan coating with gentamicin for potential applications in bone tissue engineering" in Journal of Biomedical Materials Research Part A, 108, no. 11 (2020):2175-2189,
https://doi.org/10.1002/jbm.a.36974 . .

TY  - JOUR
AU  - Stevanović, Milena
AU  - Đošić, Marija
AU  - Janković, Ana
AU  - Kojić, Vesna
AU  - Vukašinović-Sekulić, Maja
AU  - Stojanović, Jovica
AU  - Odović, Jadranka
AU  - Crevar-Sakač, Milkica
AU  - Kyong Yop, Rhee
AU  - Mišković-Stanković, Vesna
PY  - 2020
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4725
AB  - Electrophoretic deposition process (EPD) was successfully used for obtaining graphene (Gr)-reinforced composite coating based on hydroxyapatite (HAP), chitosan (CS), and antibiotic gentamicin (Gent), from aqueous suspension. The deposition process was performed as a single step process at a constant voltage (5 V, deposition time 12 min) on pure titanium foils. The influence of graphene was examined through detailed physicochemical and biological characterization. Fourier transform infrared spectroscopy, field emission scanning electron microscopy, thermogravimetric analysis, X-ray diffraction, Raman, and X-ray photoelectron analyses confirmed the formation of composite HAP/CS/Gr and HAP/CS/Gr/Gent coatings on Ti. Obtained coatings had porous, uniform, fracture-free surfaces, suggesting strong interfacial interaction between HAP, CS, and Gr. Large specific area of graphene enabled strong bonding with chitosan, acting as nanofiller throughout the polymer matrix. Gentamicin addition strongly improved the antibacterial activity of HAP/CS/Gr/Gent coating that was confirmed by antibacterial activity kinetics in suspension and agar diffusion testing, while results indicated more pronounced antibacterial effect againstStaphylococcus aureus(bactericidal, viable cells number reduction  gt 3 logarithmic units) compared toEscherichia coli(bacteriostatic, lt 3 logarithmic units).MTT assay indicated low cytotoxicity (75% cell viability) against MRC-5 and L929 (70% cell viability) tested cell lines, indicating good biocompatibility of HAP/CS/Gr/Gent coating. Therefore, electrodeposited HAP/CS/Gr/Gent coating on Ti can be considered as a prospective material for bone tissue engineering as a hard tissue implant.
PB  - Wiley, Hoboken
T2  - Journal of Biomedical Materials Research Part A
T1  - Antibacterial graphene-based hydroxyapatite/chitosan coating with gentamicin for potential applications in bone tissue engineering
EP  - 2189
IS  - 11
SP  - 2175
VL  - 108
DO  - 10.1002/jbm.a.36974
ER  - 

@article{
author = "Stevanović, Milena and Đošić, Marija and Janković, Ana and Kojić, Vesna and Vukašinović-Sekulić, Maja and Stojanović, Jovica and Odović, Jadranka and Crevar-Sakač, Milkica and Kyong Yop, Rhee and Mišković-Stanković, Vesna",
year = "2020",
abstract = "Electrophoretic deposition process (EPD) was successfully used for obtaining graphene (Gr)-reinforced composite coating based on hydroxyapatite (HAP), chitosan (CS), and antibiotic gentamicin (Gent), from aqueous suspension. The deposition process was performed as a single step process at a constant voltage (5 V, deposition time 12 min) on pure titanium foils. The influence of graphene was examined through detailed physicochemical and biological characterization. Fourier transform infrared spectroscopy, field emission scanning electron microscopy, thermogravimetric analysis, X-ray diffraction, Raman, and X-ray photoelectron analyses confirmed the formation of composite HAP/CS/Gr and HAP/CS/Gr/Gent coatings on Ti. Obtained coatings had porous, uniform, fracture-free surfaces, suggesting strong interfacial interaction between HAP, CS, and Gr. Large specific area of graphene enabled strong bonding with chitosan, acting as nanofiller throughout the polymer matrix. Gentamicin addition strongly improved the antibacterial activity of HAP/CS/Gr/Gent coating that was confirmed by antibacterial activity kinetics in suspension and agar diffusion testing, while results indicated more pronounced antibacterial effect againstStaphylococcus aureus(bactericidal, viable cells number reduction  gt 3 logarithmic units) compared toEscherichia coli(bacteriostatic, lt 3 logarithmic units).MTT assay indicated low cytotoxicity (75% cell viability) against MRC-5 and L929 (70% cell viability) tested cell lines, indicating good biocompatibility of HAP/CS/Gr/Gent coating. Therefore, electrodeposited HAP/CS/Gr/Gent coating on Ti can be considered as a prospective material for bone tissue engineering as a hard tissue implant.",
publisher = "Wiley, Hoboken",
journal = "Journal of Biomedical Materials Research Part A",
title = "Antibacterial graphene-based hydroxyapatite/chitosan coating with gentamicin for potential applications in bone tissue engineering",
pages = "2189-2175",
number = "11",
volume = "108",
doi = "10.1002/jbm.a.36974"
}

Stevanović, M., Đošić, M., Janković, A., Kojić, V., Vukašinović-Sekulić, M., Stojanović, J., Odović, J., Crevar-Sakač, M., Kyong Yop, R.,& Mišković-Stanković, V.. (2020). Antibacterial graphene-based hydroxyapatite/chitosan coating with gentamicin for potential applications in bone tissue engineering. in Journal of Biomedical Materials Research Part A
Wiley, Hoboken., 108(11), 2175-2189.
https://doi.org/10.1002/jbm.a.36974

Stevanović M, Đošić M, Janković A, Kojić V, Vukašinović-Sekulić M, Stojanović J, Odović J, Crevar-Sakač M, Kyong Yop R, Mišković-Stanković V. Antibacterial graphene-based hydroxyapatite/chitosan coating with gentamicin for potential applications in bone tissue engineering. in Journal of Biomedical Materials Research Part A. 2020;108(11):2175-2189.
doi:10.1002/jbm.a.36974 .

Stevanović, Milena, Đošić, Marija, Janković, Ana, Kojić, Vesna, Vukašinović-Sekulić, Maja, Stojanović, Jovica, Odović, Jadranka, Crevar-Sakač, Milkica, Kyong Yop, Rhee, Mišković-Stanković, Vesna, "Antibacterial graphene-based hydroxyapatite/chitosan coating with gentamicin for potential applications in bone tissue engineering" in Journal of Biomedical Materials Research Part A, 108, no. 11 (2020):2175-2189,
https://doi.org/10.1002/jbm.a.36974 . .

TY  - JOUR
AU  - Stevanović, Milena
AU  - Đošić, Marija
AU  - Janković, Ana
AU  - Nešović, Katarina
AU  - Kojić, Vesna
AU  - Stojanović, Jovica
AU  - Grujić, Svetlana
AU  - Matić-Bujagić, Ivana
AU  - Rhee, Kyong Yop
AU  - Mišković-Stanković, Vesna
PY  - 2020
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4498
AB  - The electrophoretic deposition process (EPD) was utilized to produce bioactive hydroxyapatite/chitosan (HAP/CS) and hydroxyapatite/chitosan/gentamicin (HAP/CS/Gent) coatings on titanium. The bioactivity of newly synthesized composite coatings was investigated in the simulated body fluid (SBF) and examined by X-ray diffraction, Fourier transform infrared spectroscopy, and field emission scanning electron microscopy. The obtained results revealed carbonate-substituted hydroxyapatite after immersion in SBF, emphasizing the similarity of the biomimetically grown HAP with the naturally occurring apatite in the bone. The formation of biomimetic HAP was confirmed by electrochemical impedance spectroscopy and polarization measurements, through the decrease in corrosion current density and coating capacitance values after 28-day immersion in SBF. The osseointegration ability was further validated by measuring the alkaline phosphatase activity (ALP) indicating the favorable osseopromotive properties of deposited coatings (significant increase in ALP levels for both HAP/CS (3.206 U mL(-1)) and HAP/CS/Gent (4.039 U mL(-1)) coatings, compared to the control (0.900 U mL(-1))). Drug-release kinetics was investigated in deionized water at 37 degrees C by high-performance liquid chromatography coupled with mass spectrometry. Release profiles revealed the beneficial "burst-release effect" (similar to 21% of gentamicin released in the first 48 h) as a potentially promising solution against the biofilm formation in the initial period. When tested against human and mice fibroblast cells (MRC-5 and L929), both composite coatings showed a noncytotoxic effect (viability  gt 85%), providing a promising basis for further medical application trials.
PB  - Amer Chemical Soc, Washington
T2  - ACS Omega
T1  - Assessing the Bioactivity of Gentamicin-Preloaded Hydroxyapatite/Chitosan Composite Coating on Titanium Substrate
EP  - 15445
IS  - 25
SP  - 15433
VL  - 5
DO  - 10.1021/acsomega.0c01583
ER  - 

@article{
author = "Stevanović, Milena and Đošić, Marija and Janković, Ana and Nešović, Katarina and Kojić, Vesna and Stojanović, Jovica and Grujić, Svetlana and Matić-Bujagić, Ivana and Rhee, Kyong Yop and Mišković-Stanković, Vesna",
year = "2020",
abstract = "The electrophoretic deposition process (EPD) was utilized to produce bioactive hydroxyapatite/chitosan (HAP/CS) and hydroxyapatite/chitosan/gentamicin (HAP/CS/Gent) coatings on titanium. The bioactivity of newly synthesized composite coatings was investigated in the simulated body fluid (SBF) and examined by X-ray diffraction, Fourier transform infrared spectroscopy, and field emission scanning electron microscopy. The obtained results revealed carbonate-substituted hydroxyapatite after immersion in SBF, emphasizing the similarity of the biomimetically grown HAP with the naturally occurring apatite in the bone. The formation of biomimetic HAP was confirmed by electrochemical impedance spectroscopy and polarization measurements, through the decrease in corrosion current density and coating capacitance values after 28-day immersion in SBF. The osseointegration ability was further validated by measuring the alkaline phosphatase activity (ALP) indicating the favorable osseopromotive properties of deposited coatings (significant increase in ALP levels for both HAP/CS (3.206 U mL(-1)) and HAP/CS/Gent (4.039 U mL(-1)) coatings, compared to the control (0.900 U mL(-1))). Drug-release kinetics was investigated in deionized water at 37 degrees C by high-performance liquid chromatography coupled with mass spectrometry. Release profiles revealed the beneficial "burst-release effect" (similar to 21% of gentamicin released in the first 48 h) as a potentially promising solution against the biofilm formation in the initial period. When tested against human and mice fibroblast cells (MRC-5 and L929), both composite coatings showed a noncytotoxic effect (viability  gt 85%), providing a promising basis for further medical application trials.",
publisher = "Amer Chemical Soc, Washington",
journal = "ACS Omega",
title = "Assessing the Bioactivity of Gentamicin-Preloaded Hydroxyapatite/Chitosan Composite Coating on Titanium Substrate",
pages = "15445-15433",
number = "25",
volume = "5",
doi = "10.1021/acsomega.0c01583"
}

Stevanović, M., Đošić, M., Janković, A., Nešović, K., Kojić, V., Stojanović, J., Grujić, S., Matić-Bujagić, I., Rhee, K. Y.,& Mišković-Stanković, V.. (2020). Assessing the Bioactivity of Gentamicin-Preloaded Hydroxyapatite/Chitosan Composite Coating on Titanium Substrate. in ACS Omega
Amer Chemical Soc, Washington., 5(25), 15433-15445.
https://doi.org/10.1021/acsomega.0c01583

Stevanović M, Đošić M, Janković A, Nešović K, Kojić V, Stojanović J, Grujić S, Matić-Bujagić I, Rhee KY, Mišković-Stanković V. Assessing the Bioactivity of Gentamicin-Preloaded Hydroxyapatite/Chitosan Composite Coating on Titanium Substrate. in ACS Omega. 2020;5(25):15433-15445.
doi:10.1021/acsomega.0c01583 .

Stevanović, Milena, Đošić, Marija, Janković, Ana, Nešović, Katarina, Kojić, Vesna, Stojanović, Jovica, Grujić, Svetlana, Matić-Bujagić, Ivana, Rhee, Kyong Yop, Mišković-Stanković, Vesna, "Assessing the Bioactivity of Gentamicin-Preloaded Hydroxyapatite/Chitosan Composite Coating on Titanium Substrate" in ACS Omega, 5, no. 25 (2020):15433-15445,
https://doi.org/10.1021/acsomega.0c01583 . .

TY  - JOUR
AU  - Nešović, Katarina
AU  - Janković, Ana
AU  - Perić-Grujić, Aleksandra
AU  - Vukašinović-Sekulić, Maja
AU  - Radetić, Tamara
AU  - Živković, Ljiljana
AU  - Park, Soo Jin
AU  - Rhee, Kyong Yop
AU  - Mišković-Stanković, Vesna
PY  - 2019
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4239
AB  - Silver nanoparticles (AgNPs) were synthesized by in situ electrochemical reduction of Ag+ ions in poly (vinyl alcohol)/chitosan/graphene (PVA/CHI/Gr) hydrogel matrices with different concentrations of chitosan. The physicochemical properties of nanocomposite hydrogels were investigated by UV-vis spectroscopy (UV-vis), dynamic light scattering (DLS), transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), field-emission scanning electron microscopy (FE-SEM) and Fourier transform infrared spectroscopy (FT-IR), thermal characteristics were determined by differential scanning calorimetry (DSC) and mechanical properties were measured by tensile test. The swelling studies were carried out in phosphate buffer to simulate natural physiological environment and data were fitted by several kinetic models to determine the diffusion mechanism and diffusion coefficients of the swelling medium through the hydrogel matrices. It was shown that the presence of silver nanoparticles increased the uptake capability and equilibrium swelling degree of the composite hydrogels. The antibacterial activity was confirmed against Escherichia coli and Staphylococcus aureus, while the hydrogels without AgNPs exhibited antibacterial properties due to the presence of chitosan. With the addition of AgNPs, the samples showed stronger activity and fast reduction in the number of colonies, confirming the synergistic effect of chitosan and AgNPs on the antibacterial activity.
PB  - Elsevier Science Inc, New York
T2  - Journal of Industrial and Engineering Chemistry
T1  - Kinetic models of swelling and thermal stability of silver/poly(vinyl Check far alcohol)/chitosan/graphene hydrogels
EP  - 96
SP  - 83
VL  - 77
DO  - 10.1016/j.jiec.2019.04.022
ER  - 

@article{
author = "Nešović, Katarina and Janković, Ana and Perić-Grujić, Aleksandra and Vukašinović-Sekulić, Maja and Radetić, Tamara and Živković, Ljiljana and Park, Soo Jin and Rhee, Kyong Yop and Mišković-Stanković, Vesna",
year = "2019",
abstract = "Silver nanoparticles (AgNPs) were synthesized by in situ electrochemical reduction of Ag+ ions in poly (vinyl alcohol)/chitosan/graphene (PVA/CHI/Gr) hydrogel matrices with different concentrations of chitosan. The physicochemical properties of nanocomposite hydrogels were investigated by UV-vis spectroscopy (UV-vis), dynamic light scattering (DLS), transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), field-emission scanning electron microscopy (FE-SEM) and Fourier transform infrared spectroscopy (FT-IR), thermal characteristics were determined by differential scanning calorimetry (DSC) and mechanical properties were measured by tensile test. The swelling studies were carried out in phosphate buffer to simulate natural physiological environment and data were fitted by several kinetic models to determine the diffusion mechanism and diffusion coefficients of the swelling medium through the hydrogel matrices. It was shown that the presence of silver nanoparticles increased the uptake capability and equilibrium swelling degree of the composite hydrogels. The antibacterial activity was confirmed against Escherichia coli and Staphylococcus aureus, while the hydrogels without AgNPs exhibited antibacterial properties due to the presence of chitosan. With the addition of AgNPs, the samples showed stronger activity and fast reduction in the number of colonies, confirming the synergistic effect of chitosan and AgNPs on the antibacterial activity.",
publisher = "Elsevier Science Inc, New York",
journal = "Journal of Industrial and Engineering Chemistry",
title = "Kinetic models of swelling and thermal stability of silver/poly(vinyl Check far alcohol)/chitosan/graphene hydrogels",
pages = "96-83",
volume = "77",
doi = "10.1016/j.jiec.2019.04.022"
}

Nešović, K., Janković, A., Perić-Grujić, A., Vukašinović-Sekulić, M., Radetić, T., Živković, L., Park, S. J., Rhee, K. Y.,& Mišković-Stanković, V.. (2019). Kinetic models of swelling and thermal stability of silver/poly(vinyl Check far alcohol)/chitosan/graphene hydrogels. in Journal of Industrial and Engineering Chemistry
Elsevier Science Inc, New York., 77, 83-96.
https://doi.org/10.1016/j.jiec.2019.04.022

Nešović K, Janković A, Perić-Grujić A, Vukašinović-Sekulić M, Radetić T, Živković L, Park SJ, Rhee KY, Mišković-Stanković V. Kinetic models of swelling and thermal stability of silver/poly(vinyl Check far alcohol)/chitosan/graphene hydrogels. in Journal of Industrial and Engineering Chemistry. 2019;77:83-96.
doi:10.1016/j.jiec.2019.04.022 .

Nešović, Katarina, Janković, Ana, Perić-Grujić, Aleksandra, Vukašinović-Sekulić, Maja, Radetić, Tamara, Živković, Ljiljana, Park, Soo Jin, Rhee, Kyong Yop, Mišković-Stanković, Vesna, "Kinetic models of swelling and thermal stability of silver/poly(vinyl Check far alcohol)/chitosan/graphene hydrogels" in Journal of Industrial and Engineering Chemistry, 77 (2019):83-96,
https://doi.org/10.1016/j.jiec.2019.04.022 . .

TY  - JOUR
AU  - Nešović, Katarina
AU  - Janković, Ana
AU  - Perić-Grujić, Aleksandra
AU  - Vukašinović-Sekulić, Maja
AU  - Radetić, Tamara
AU  - Živković, Ljiljana
AU  - Park, Soo Jin
AU  - Rhee, Kyong Yop
AU  - Mišković-Stanković, Vesna
PY  - 2019
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5044
AB  - Silver nanoparticles (AgNPs) were synthesized by in situ electrochemical reduction of Ag+ ions in poly(vinyl alcohol)/chitosan/graphene (PVA/CHI/Gr) hydrogel matrices with different concentrations of chitosan. The physicochemical properties of nanocomposite hydrogels were investigated by UV–vis spectroscopy (UV–vis), dynamic light scattering (DLS), transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), field-emission scanning electron microscopy (FE-SEM) and Fourier-transform infrared spectroscopy (FT-IR), thermal characteristics were determined by differential scanning calorimetry (DSC) and mechanical properties were measured by tensile test. The swelling studies were carried out in phosphate buffer to simulate natural physiological environment and data were fitted by several kinetic models to determine the diffusion mechanism and diffusion coefficients of the swelling medium through the hydrogel matrices. It was shown that the presence of silver nanoparticles increased the uptake capability and equilibrium swelling degree of the composite hydrogels. The antibacterial activity was confirmed against Escherichia coli and Staphylococcus aureus, while the hydrogels without AgNPs exhibited antibacterial properties due to the presence of chitosan. With the addition of AgNPs, the samples showed stronger activity and fast reduction in the number of colonies, confirming the synergistic effect of chitosan and AgNPs on the antibacterial activity.
PB  - Korean Society of Industrial Engineering Chemistry
T2  - Journal of Industrial and Engineering Chemistry
T1  - Kinetic models of swelling and thermal stability of silver/poly(vinyl alcohol)/chitosan/graphene hydrogels
EP  - 96
SP  - 83
VL  - 77
DO  - 10.1016/j.jiec.2019.04.022
ER  - 

@article{
author = "Nešović, Katarina and Janković, Ana and Perić-Grujić, Aleksandra and Vukašinović-Sekulić, Maja and Radetić, Tamara and Živković, Ljiljana and Park, Soo Jin and Rhee, Kyong Yop and Mišković-Stanković, Vesna",
year = "2019",
abstract = "Silver nanoparticles (AgNPs) were synthesized by in situ electrochemical reduction of Ag+ ions in poly(vinyl alcohol)/chitosan/graphene (PVA/CHI/Gr) hydrogel matrices with different concentrations of chitosan. The physicochemical properties of nanocomposite hydrogels were investigated by UV–vis spectroscopy (UV–vis), dynamic light scattering (DLS), transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), field-emission scanning electron microscopy (FE-SEM) and Fourier-transform infrared spectroscopy (FT-IR), thermal characteristics were determined by differential scanning calorimetry (DSC) and mechanical properties were measured by tensile test. The swelling studies were carried out in phosphate buffer to simulate natural physiological environment and data were fitted by several kinetic models to determine the diffusion mechanism and diffusion coefficients of the swelling medium through the hydrogel matrices. It was shown that the presence of silver nanoparticles increased the uptake capability and equilibrium swelling degree of the composite hydrogels. The antibacterial activity was confirmed against Escherichia coli and Staphylococcus aureus, while the hydrogels without AgNPs exhibited antibacterial properties due to the presence of chitosan. With the addition of AgNPs, the samples showed stronger activity and fast reduction in the number of colonies, confirming the synergistic effect of chitosan and AgNPs on the antibacterial activity.",
publisher = "Korean Society of Industrial Engineering Chemistry",
journal = "Journal of Industrial and Engineering Chemistry",
title = "Kinetic models of swelling and thermal stability of silver/poly(vinyl alcohol)/chitosan/graphene hydrogels",
pages = "96-83",
volume = "77",
doi = "10.1016/j.jiec.2019.04.022"
}

Nešović, K., Janković, A., Perić-Grujić, A., Vukašinović-Sekulić, M., Radetić, T., Živković, L., Park, S. J., Rhee, K. Y.,& Mišković-Stanković, V.. (2019). Kinetic models of swelling and thermal stability of silver/poly(vinyl alcohol)/chitosan/graphene hydrogels. in Journal of Industrial and Engineering Chemistry
Korean Society of Industrial Engineering Chemistry., 77, 83-96.
https://doi.org/10.1016/j.jiec.2019.04.022

Nešović K, Janković A, Perić-Grujić A, Vukašinović-Sekulić M, Radetić T, Živković L, Park SJ, Rhee KY, Mišković-Stanković V. Kinetic models of swelling and thermal stability of silver/poly(vinyl alcohol)/chitosan/graphene hydrogels. in Journal of Industrial and Engineering Chemistry. 2019;77:83-96.
doi:10.1016/j.jiec.2019.04.022 .

Nešović, Katarina, Janković, Ana, Perić-Grujić, Aleksandra, Vukašinović-Sekulić, Maja, Radetić, Tamara, Živković, Ljiljana, Park, Soo Jin, Rhee, Kyong Yop, Mišković-Stanković, Vesna, "Kinetic models of swelling and thermal stability of silver/poly(vinyl alcohol)/chitosan/graphene hydrogels" in Journal of Industrial and Engineering Chemistry, 77 (2019):83-96,
https://doi.org/10.1016/j.jiec.2019.04.022 . .

TY  - CONF
AU  - Mišković-Stanković, Vesna
AU  - Nešović, Katarina
AU  - Janković, Ana
AU  - Radetić, Tamara
AU  - Perić-Grujić, Aleksandra
AU  - Vukašinović-Sekulić, Maja
AU  - Kojić, Vesna
AU  - Yop Rhee, Kyong
PY  - 2019
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7245
AB  - In recent times, polymer-based hydrogel materials have presented themselves as excellent candidates for
new-generation wound dressings with improved properties, such as high sorption ability, good mechanical
properties and low adhesiveness [1]. Additionally, cross linked hydrogel matrices serve as excellent carriers
for controlled release of antibacterial agents, such as silver nanoparticles (Ag/NPs), which are preferred over
conventional antibiotics due to multi-phase mechanism of action and low susceptibility to induce bacterial
resistance [2]. In this work, we aim to produce novel silver/poly(vinyl alcohol)/chitosan (Ag/PVA/CHI)
hydrogels for wound dressing applications. The electrochemical route for AgNPs synthesis provided facile
and green method for the reduction of Ag+
ions inside the hydrogel matrices, without the need to use toxic
chemical reducing agents [3]. The effect of chitosan content on the synthesis yield, antibacterial properties,
swelling and release kinetics was investigated. The formation of AgNPs was confirmed using UV-visible
spectroscopy through the appearance of plasmon resonant peaks at around 400 nm (Figure 1a), whereas
transmission electron microscopy (TEM) proved the incorporation of both single and polycrystalline spherical
AgNPs with diameters of 5-10 nm (Figure 1b). The swelling and silver release kinetics were investigated in
modified phosphate buffer solutions (pH 7.4) at 37 °C to mimic physiological conditions. The obtained
swelling isotherms and release profiles were fitted with different models to obtain kinetic and diffusion
parameters (Figure 1c). The antibacterial activity was evaluated against Staphylococcus aureus TL and
Escherichia coli ATCC 25922 bacterial strains using an in-suspension test, and non-toxicity of both silvercontaining and silver-free hydrogels was proved by MTT cytotoxicity test. The obtained results confirmed
strong potential of Ag/PVA/CHI hydrogels for biomedical applications.
PB  - International Association of Physical Chemists
C3  - Book of abstracts / 7th Regional Symposium on Electrochemistry – South East Europe & 8th Kurt Schwabe Symposium, Split, Croatia, May 27-30, 2019
T1  - Poly(vinyl alcohol)/chitosan hydrogels with electrochemically synthesized silver nanoparticles for wound dressing applications
SP  - 121
UR  - https://hdl.handle.net/21.15107/rcub_technorep_7245
ER  - 

@conference{
author = "Mišković-Stanković, Vesna and Nešović, Katarina and Janković, Ana and Radetić, Tamara and Perić-Grujić, Aleksandra and Vukašinović-Sekulić, Maja and Kojić, Vesna and Yop Rhee, Kyong",
year = "2019",
abstract = "In recent times, polymer-based hydrogel materials have presented themselves as excellent candidates for
new-generation wound dressings with improved properties, such as high sorption ability, good mechanical
properties and low adhesiveness [1]. Additionally, cross linked hydrogel matrices serve as excellent carriers
for controlled release of antibacterial agents, such as silver nanoparticles (Ag/NPs), which are preferred over
conventional antibiotics due to multi-phase mechanism of action and low susceptibility to induce bacterial
resistance [2]. In this work, we aim to produce novel silver/poly(vinyl alcohol)/chitosan (Ag/PVA/CHI)
hydrogels for wound dressing applications. The electrochemical route for AgNPs synthesis provided facile
and green method for the reduction of Ag+
ions inside the hydrogel matrices, without the need to use toxic
chemical reducing agents [3]. The effect of chitosan content on the synthesis yield, antibacterial properties,
swelling and release kinetics was investigated. The formation of AgNPs was confirmed using UV-visible
spectroscopy through the appearance of plasmon resonant peaks at around 400 nm (Figure 1a), whereas
transmission electron microscopy (TEM) proved the incorporation of both single and polycrystalline spherical
AgNPs with diameters of 5-10 nm (Figure 1b). The swelling and silver release kinetics were investigated in
modified phosphate buffer solutions (pH 7.4) at 37 °C to mimic physiological conditions. The obtained
swelling isotherms and release profiles were fitted with different models to obtain kinetic and diffusion
parameters (Figure 1c). The antibacterial activity was evaluated against Staphylococcus aureus TL and
Escherichia coli ATCC 25922 bacterial strains using an in-suspension test, and non-toxicity of both silvercontaining and silver-free hydrogels was proved by MTT cytotoxicity test. The obtained results confirmed
strong potential of Ag/PVA/CHI hydrogels for biomedical applications.",
publisher = "International Association of Physical Chemists",
journal = "Book of abstracts / 7th Regional Symposium on Electrochemistry – South East Europe & 8th Kurt Schwabe Symposium, Split, Croatia, May 27-30, 2019",
title = "Poly(vinyl alcohol)/chitosan hydrogels with electrochemically synthesized silver nanoparticles for wound dressing applications",
pages = "121",
url = "https://hdl.handle.net/21.15107/rcub_technorep_7245"
}

Mišković-Stanković, V., Nešović, K., Janković, A., Radetić, T., Perić-Grujić, A., Vukašinović-Sekulić, M., Kojić, V.,& Yop Rhee, K.. (2019). Poly(vinyl alcohol)/chitosan hydrogels with electrochemically synthesized silver nanoparticles for wound dressing applications. in Book of abstracts / 7th Regional Symposium on Electrochemistry – South East Europe & 8th Kurt Schwabe Symposium, Split, Croatia, May 27-30, 2019
International Association of Physical Chemists., 121.
https://hdl.handle.net/21.15107/rcub_technorep_7245

Mišković-Stanković V, Nešović K, Janković A, Radetić T, Perić-Grujić A, Vukašinović-Sekulić M, Kojić V, Yop Rhee K. Poly(vinyl alcohol)/chitosan hydrogels with electrochemically synthesized silver nanoparticles for wound dressing applications. in Book of abstracts / 7th Regional Symposium on Electrochemistry – South East Europe & 8th Kurt Schwabe Symposium, Split, Croatia, May 27-30, 2019. 2019;:121.
https://hdl.handle.net/21.15107/rcub_technorep_7245 .

Mišković-Stanković, Vesna, Nešović, Katarina, Janković, Ana, Radetić, Tamara, Perić-Grujić, Aleksandra, Vukašinović-Sekulić, Maja, Kojić, Vesna, Yop Rhee, Kyong, "Poly(vinyl alcohol)/chitosan hydrogels with electrochemically synthesized silver nanoparticles for wound dressing applications" in Book of abstracts / 7th Regional Symposium on Electrochemistry – South East Europe & 8th Kurt Schwabe Symposium, Split, Croatia, May 27-30, 2019 (2019):121,
https://hdl.handle.net/21.15107/rcub_technorep_7245 .

TY  - JOUR
AU  - Nešović, Katarina
AU  - Janković, Ana
AU  - Radetić, Tamara
AU  - Vukašinović-Sekulić, Maja
AU  - Kojić, Vesna
AU  - Živković, Ljiljana
AU  - Perić-Grujić, Aleksandra
AU  - Rhee, Kyong Yop
AU  - Mišković-Stanković, Vesna
PY  - 2019
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4113
AB  - In this work, we provide a facile path for the preparation of wound dressing materials with improved characteristics based on biocompatible chitosan and poly(vinyl alcohol) hydrogels with embedded silver nanoparticles (AgNPs) as a potent antimicrobial agent. The hydrogels were prepared via freezing-thawing method, whereas AgNPs were synthesized via an in situ electrochemical route. The excellent physicochemical properties of the obtained nanocomposite hydrogels were validated using different spectroscopic and microscopic techniques. The formation of sub-10 nm AgNPs was confirmed by UV-visible spectroscopy, dynamic light scattering and transmission electron microscopy. Swelling and silver release profiles were validated using several diffusion models. The hydrogels were proven to be non-cytotoxic using MTT test and possessed powerful antibacterial activity against Staphylococcus aureus and Escherichia coli, as determined by test in suspension, which affirmed their feasibility for wound dressing applications.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - European Polymer Journal
T1  - Chitosan-based hydrogel wound dressings with electrochemically incorporated silver nanoparticles - In vitro study
VL  - 121
DO  - 10.1016/j.eurpolymj.2019.109257
ER  - 

@article{
author = "Nešović, Katarina and Janković, Ana and Radetić, Tamara and Vukašinović-Sekulić, Maja and Kojić, Vesna and Živković, Ljiljana and Perić-Grujić, Aleksandra and Rhee, Kyong Yop and Mišković-Stanković, Vesna",
year = "2019",
abstract = "In this work, we provide a facile path for the preparation of wound dressing materials with improved characteristics based on biocompatible chitosan and poly(vinyl alcohol) hydrogels with embedded silver nanoparticles (AgNPs) as a potent antimicrobial agent. The hydrogels were prepared via freezing-thawing method, whereas AgNPs were synthesized via an in situ electrochemical route. The excellent physicochemical properties of the obtained nanocomposite hydrogels were validated using different spectroscopic and microscopic techniques. The formation of sub-10 nm AgNPs was confirmed by UV-visible spectroscopy, dynamic light scattering and transmission electron microscopy. Swelling and silver release profiles were validated using several diffusion models. The hydrogels were proven to be non-cytotoxic using MTT test and possessed powerful antibacterial activity against Staphylococcus aureus and Escherichia coli, as determined by test in suspension, which affirmed their feasibility for wound dressing applications.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "European Polymer Journal",
title = "Chitosan-based hydrogel wound dressings with electrochemically incorporated silver nanoparticles - In vitro study",
volume = "121",
doi = "10.1016/j.eurpolymj.2019.109257"
}

Nešović, K., Janković, A., Radetić, T., Vukašinović-Sekulić, M., Kojić, V., Živković, L., Perić-Grujić, A., Rhee, K. Y.,& Mišković-Stanković, V.. (2019). Chitosan-based hydrogel wound dressings with electrochemically incorporated silver nanoparticles - In vitro study. in European Polymer Journal
Pergamon-Elsevier Science Ltd, Oxford., 121.
https://doi.org/10.1016/j.eurpolymj.2019.109257

Nešović K, Janković A, Radetić T, Vukašinović-Sekulić M, Kojić V, Živković L, Perić-Grujić A, Rhee KY, Mišković-Stanković V. Chitosan-based hydrogel wound dressings with electrochemically incorporated silver nanoparticles - In vitro study. in European Polymer Journal. 2019;121.
doi:10.1016/j.eurpolymj.2019.109257 .

Nešović, Katarina, Janković, Ana, Radetić, Tamara, Vukašinović-Sekulić, Maja, Kojić, Vesna, Živković, Ljiljana, Perić-Grujić, Aleksandra, Rhee, Kyong Yop, Mišković-Stanković, Vesna, "Chitosan-based hydrogel wound dressings with electrochemically incorporated silver nanoparticles - In vitro study" in European Polymer Journal, 121 (2019),
https://doi.org/10.1016/j.eurpolymj.2019.109257 . .

TY  - CONF
AU  - Nešović, Katarina
AU  - Janković, Ana
AU  - Radetić, Tamara
AU  - Kojić, Vesna
AU  - Mišković-Stanković, Vesna
PY  - 2019
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7246
AB  - Nauka i inženjerstvo biomaterijala u poslednje vreme pokazuju sve veću težnju ka
pronalaženju novih materijala za obloge za rane, gde su naročito važna poboljšana svojstva
kao što su apsorpciona moć, mehanička svojstva i niska adhezivnost. Umrežene matrice
hidrogelova su odlični potencijalni nosači za kontrolisano otpuštanje antibakterijskih
agenasa, kao što su nanočestice srebra. Hitozan je posebno interesantna komponenta
obloga za rane, zbog svoje prirodne antibakterijske aktivnosti i sposobnosti stabilizacije
nanočestica. Cilj ovog rada je priprema novih obloga za rane na bazi polivinil-alkohola i
hitozana sa elektrohemijski sintetisanim nanočesticama srebra, u obliku hidrogela. Dobijene
su sferne nanočestice srebra, prečnika oko 5-10 nm, što je potvrđeno UV-vidljivom
spektroskopijom i transmisionom elektronskom mikroskopijom. Bubrenje i otpuštanje
srebra je praćeno u fosfatnom puferu koji imitira fiziološke uslove, dok je antibakterijska
aktivnost potvrđena na bakterijskim sojevima Staphylococcus aureus i Escherichia coli.
Dobijeni netoksični hidrogelovi (što je utvrđeno MTT testom) imaju izuzetan potencijal za
primene kao obloge za rane nove generacije.
AB  - Recently, the biomaterials science and engineering fields have seen a shift towards novel
hydrogel materials, especially for wound dressing applications where improved properties,
such as high sorption ability, good mechanical properties and low adhesiveness are of
utmost importance. The cross linked hydrogel matrices are excellent potential carriers for
controlled release of antibacterial agents, such as silver nanoparticles (AgNPs). Chitosan is
an especially attractive option for wound dressing applications, because of its intrinsic
antibacterial activity and the ability to stabilize AgNPs. The aim of this work is the production
of poly(vinyl alcohol) and chitosan based hydrogel matrices with electrochemically
synthesized AgNPs for wound dressing materials applications. The obtained AgNPs were
spherical with 5-10 nm diameters, as confirmed by UV-visible spectroscopy and
transmission electron microscopy. The swelling and release behaviors of AgNP-embedded
hydrogels were evaluated in phosphate buffer, mimicking physiological environment, and
the antibacterial activity was confirmed against both Staphylococcus aureus and Escherichia
coli. The obtained non-toxic hydrogel materials (as affirmed by MTT test) have excellent
potential to be used as novel antibacterial wound dressings.
PB  - Beograd : Srpsko hemijsko društvo
C3  - Kratki izvodi radova / 56. savetovanje Srpskog hemijskog društva , Niš 7. i 8. juni 2019
T1  - Novi materijali za obloge za rane sa elektrohemijski sintetisanim nanočesticama srebra
T1  - Novel wound dressing materials containing electrochemically synthesized silver nanoparticles
SP  - 27
UR  - https://hdl.handle.net/21.15107/rcub_technorep_7246
ER  - 

@conference{
author = "Nešović, Katarina and Janković, Ana and Radetić, Tamara and Kojić, Vesna and Mišković-Stanković, Vesna",
year = "2019",
abstract = "Nauka i inženjerstvo biomaterijala u poslednje vreme pokazuju sve veću težnju ka
pronalaženju novih materijala za obloge za rane, gde su naročito važna poboljšana svojstva
kao što su apsorpciona moć, mehanička svojstva i niska adhezivnost. Umrežene matrice
hidrogelova su odlični potencijalni nosači za kontrolisano otpuštanje antibakterijskih
agenasa, kao što su nanočestice srebra. Hitozan je posebno interesantna komponenta
obloga za rane, zbog svoje prirodne antibakterijske aktivnosti i sposobnosti stabilizacije
nanočestica. Cilj ovog rada je priprema novih obloga za rane na bazi polivinil-alkohola i
hitozana sa elektrohemijski sintetisanim nanočesticama srebra, u obliku hidrogela. Dobijene
su sferne nanočestice srebra, prečnika oko 5-10 nm, što je potvrđeno UV-vidljivom
spektroskopijom i transmisionom elektronskom mikroskopijom. Bubrenje i otpuštanje
srebra je praćeno u fosfatnom puferu koji imitira fiziološke uslove, dok je antibakterijska
aktivnost potvrđena na bakterijskim sojevima Staphylococcus aureus i Escherichia coli.
Dobijeni netoksični hidrogelovi (što je utvrđeno MTT testom) imaju izuzetan potencijal za
primene kao obloge za rane nove generacije., Recently, the biomaterials science and engineering fields have seen a shift towards novel
hydrogel materials, especially for wound dressing applications where improved properties,
such as high sorption ability, good mechanical properties and low adhesiveness are of
utmost importance. The cross linked hydrogel matrices are excellent potential carriers for
controlled release of antibacterial agents, such as silver nanoparticles (AgNPs). Chitosan is
an especially attractive option for wound dressing applications, because of its intrinsic
antibacterial activity and the ability to stabilize AgNPs. The aim of this work is the production
of poly(vinyl alcohol) and chitosan based hydrogel matrices with electrochemically
synthesized AgNPs for wound dressing materials applications. The obtained AgNPs were
spherical with 5-10 nm diameters, as confirmed by UV-visible spectroscopy and
transmission electron microscopy. The swelling and release behaviors of AgNP-embedded
hydrogels were evaluated in phosphate buffer, mimicking physiological environment, and
the antibacterial activity was confirmed against both Staphylococcus aureus and Escherichia
coli. The obtained non-toxic hydrogel materials (as affirmed by MTT test) have excellent
potential to be used as novel antibacterial wound dressings.",
publisher = "Beograd : Srpsko hemijsko društvo",
journal = "Kratki izvodi radova / 56. savetovanje Srpskog hemijskog društva , Niš 7. i 8. juni 2019",
title = "Novi materijali za obloge za rane sa elektrohemijski sintetisanim nanočesticama srebra, Novel wound dressing materials containing electrochemically synthesized silver nanoparticles",
pages = "27",
url = "https://hdl.handle.net/21.15107/rcub_technorep_7246"
}

Nešović, K., Janković, A., Radetić, T., Kojić, V.,& Mišković-Stanković, V.. (2019). Novi materijali za obloge za rane sa elektrohemijski sintetisanim nanočesticama srebra. in Kratki izvodi radova / 56. savetovanje Srpskog hemijskog društva , Niš 7. i 8. juni 2019
Beograd : Srpsko hemijsko društvo., 27.
https://hdl.handle.net/21.15107/rcub_technorep_7246

Nešović K, Janković A, Radetić T, Kojić V, Mišković-Stanković V. Novi materijali za obloge za rane sa elektrohemijski sintetisanim nanočesticama srebra. in Kratki izvodi radova / 56. savetovanje Srpskog hemijskog društva , Niš 7. i 8. juni 2019. 2019;:27.
https://hdl.handle.net/21.15107/rcub_technorep_7246 .

Nešović, Katarina, Janković, Ana, Radetić, Tamara, Kojić, Vesna, Mišković-Stanković, Vesna, "Novi materijali za obloge za rane sa elektrohemijski sintetisanim nanočesticama srebra" in Kratki izvodi radova / 56. savetovanje Srpskog hemijskog društva , Niš 7. i 8. juni 2019 (2019):27,
https://hdl.handle.net/21.15107/rcub_technorep_7246 .

TY  - JOUR
AU  - Stevanović, Milena
AU  - Đošić, Marija
AU  - Janković, Ana
AU  - Rhee, Kyong Yop
AU  - Mišković-Stanković, Vesna
PY  - 2019
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4101
AB  - Increasing need for improved, compatible bone tissue implants led to the intensive research of novel biomaterials, especially hydroxyapatite (HAP)-based composite materials on titanium and titanium alloy surfaces. Owing to its excellent biocompatibility and osteoinductivity properties, hydroxyapatite is often used as part of composite biomaterials aimed for orthopedic implant applications. In order to overcome persistent problems of bacterial infection, various antimicrobial agents and materials and their incorporation in such medical devices were investigated. This paper represents a comprehensive review of single-step electrodeposition on titanium of hydroxyapatite/chitosan/graphene composite coatings loaded with silver and antibiotic gentamicin as antibacterial agents. The improvement of mechanical and adhesive properties of deposited composite coatings was achieved by graphene and chitosan addition, while desirable antibacterial properties were introduced by including antibiotic gentamicin and silver. The biocompatibility of electrodeposited HAP and HAP-based composite coatings was evaluated by MTT testing, indicating a non-cytotoxic effect and high potential for future medical use as orthopedic implant coating.
PB  - Srpsko hemijsko društvo, Beograd
T2  - Journal of the Serbian Chemical Society
T1  - Electrophoretically deposited hydroxyapatite-based composite coatings loaded with silver and gentamicin as antibacterial agents
EP  - 1304
IS  - 11
SP  - 1287
VL  - 84
DO  - 10.2298/JSC190821092S
ER  - 

@article{
author = "Stevanović, Milena and Đošić, Marija and Janković, Ana and Rhee, Kyong Yop and Mišković-Stanković, Vesna",
year = "2019",
abstract = "Increasing need for improved, compatible bone tissue implants led to the intensive research of novel biomaterials, especially hydroxyapatite (HAP)-based composite materials on titanium and titanium alloy surfaces. Owing to its excellent biocompatibility and osteoinductivity properties, hydroxyapatite is often used as part of composite biomaterials aimed for orthopedic implant applications. In order to overcome persistent problems of bacterial infection, various antimicrobial agents and materials and their incorporation in such medical devices were investigated. This paper represents a comprehensive review of single-step electrodeposition on titanium of hydroxyapatite/chitosan/graphene composite coatings loaded with silver and antibiotic gentamicin as antibacterial agents. The improvement of mechanical and adhesive properties of deposited composite coatings was achieved by graphene and chitosan addition, while desirable antibacterial properties were introduced by including antibiotic gentamicin and silver. The biocompatibility of electrodeposited HAP and HAP-based composite coatings was evaluated by MTT testing, indicating a non-cytotoxic effect and high potential for future medical use as orthopedic implant coating.",
publisher = "Srpsko hemijsko društvo, Beograd",
journal = "Journal of the Serbian Chemical Society",
title = "Electrophoretically deposited hydroxyapatite-based composite coatings loaded with silver and gentamicin as antibacterial agents",
pages = "1304-1287",
number = "11",
volume = "84",
doi = "10.2298/JSC190821092S"
}

Stevanović, M., Đošić, M., Janković, A., Rhee, K. Y.,& Mišković-Stanković, V.. (2019). Electrophoretically deposited hydroxyapatite-based composite coatings loaded with silver and gentamicin as antibacterial agents. in Journal of the Serbian Chemical Society
Srpsko hemijsko društvo, Beograd., 84(11), 1287-1304.
https://doi.org/10.2298/JSC190821092S

Stevanović M, Đošić M, Janković A, Rhee KY, Mišković-Stanković V. Electrophoretically deposited hydroxyapatite-based composite coatings loaded with silver and gentamicin as antibacterial agents. in Journal of the Serbian Chemical Society. 2019;84(11):1287-1304.
doi:10.2298/JSC190821092S .

Stevanović, Milena, Đošić, Marija, Janković, Ana, Rhee, Kyong Yop, Mišković-Stanković, Vesna, "Electrophoretically deposited hydroxyapatite-based composite coatings loaded with silver and gentamicin as antibacterial agents" in Journal of the Serbian Chemical Society, 84, no. 11 (2019):1287-1304,
https://doi.org/10.2298/JSC190821092S . .

TY  - CONF
AU  - Nešović, Katarina
AU  - Janković, Ana
AU  - Vukašinović-Sekulić, Maja
AU  - Perić-Grujić, Aleksandra
AU  - Mišković-Stanković, Vesna
PY  - 2018
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7386
AB  - In this work, we prepared hydrogels based on PVA, CHI and Gr, and used them
as a porous matrix to synthesize silver nanoparticles by an in situ electrochemical
method [3,4]. The hydrogels were prepared from PVA/CHI/Gr colloid dispersions
using a simple and effective freezing-thawing method for gelation, without the use
of chemical cross linkers. The hydrogels were then swollen in AgNO3 solution to
saturate with Ag+ ions and subsequently subjected to constant-voltage electrical
current, to reduce silver ions and to obtain AgNPs. This electrochemical method
is fast and green, as there is no need for the use of any (potentially toxic) chemical
cross linkers and reducing agents, rendering the final product non-toxic and safe for
medical applications.
PB  - Belgrade : Serbian Academy of Sciences and Arts
C3  - Program ; Book of Abstracts / First International Conference on Electron Microscopy of Nanostructures ELMINA 2018, August 27-29, 2018, Belgrade, Serbia = Прва међународна конференција о електронској микроскопији наноструктура ELMINA 2018, 27-29 август 2018. Београд, Србија
T1  - Nanocomposite Hydrogels Based on Poly(vinyl alcohol) and Chitosan with Silver Nanoparticles and Graphene Aimed for Wound Dressing Applications
EP  - 242
SP  - 240
UR  - https://hdl.handle.net/21.15107/rcub_technorep_7386
ER  - 

@conference{
author = "Nešović, Katarina and Janković, Ana and Vukašinović-Sekulić, Maja and Perić-Grujić, Aleksandra and Mišković-Stanković, Vesna",
year = "2018",
abstract = "In this work, we prepared hydrogels based on PVA, CHI and Gr, and used them
as a porous matrix to synthesize silver nanoparticles by an in situ electrochemical
method [3,4]. The hydrogels were prepared from PVA/CHI/Gr colloid dispersions
using a simple and effective freezing-thawing method for gelation, without the use
of chemical cross linkers. The hydrogels were then swollen in AgNO3 solution to
saturate with Ag+ ions and subsequently subjected to constant-voltage electrical
current, to reduce silver ions and to obtain AgNPs. This electrochemical method
is fast and green, as there is no need for the use of any (potentially toxic) chemical
cross linkers and reducing agents, rendering the final product non-toxic and safe for
medical applications.",
publisher = "Belgrade : Serbian Academy of Sciences and Arts",
journal = "Program ; Book of Abstracts / First International Conference on Electron Microscopy of Nanostructures ELMINA 2018, August 27-29, 2018, Belgrade, Serbia = Прва међународна конференција о електронској микроскопији наноструктура ELMINA 2018, 27-29 август 2018. Београд, Србија",
title = "Nanocomposite Hydrogels Based on Poly(vinyl alcohol) and Chitosan with Silver Nanoparticles and Graphene Aimed for Wound Dressing Applications",
pages = "242-240",
url = "https://hdl.handle.net/21.15107/rcub_technorep_7386"
}

Nešović, K., Janković, A., Vukašinović-Sekulić, M., Perić-Grujić, A.,& Mišković-Stanković, V.. (2018). Nanocomposite Hydrogels Based on Poly(vinyl alcohol) and Chitosan with Silver Nanoparticles and Graphene Aimed for Wound Dressing Applications. in Program ; Book of Abstracts / First International Conference on Electron Microscopy of Nanostructures ELMINA 2018, August 27-29, 2018, Belgrade, Serbia = Прва међународна конференција о електронској микроскопији наноструктура ELMINA 2018, 27-29 август 2018. Београд, Србија
Belgrade : Serbian Academy of Sciences and Arts., 240-242.
https://hdl.handle.net/21.15107/rcub_technorep_7386

Nešović K, Janković A, Vukašinović-Sekulić M, Perić-Grujić A, Mišković-Stanković V. Nanocomposite Hydrogels Based on Poly(vinyl alcohol) and Chitosan with Silver Nanoparticles and Graphene Aimed for Wound Dressing Applications. in Program ; Book of Abstracts / First International Conference on Electron Microscopy of Nanostructures ELMINA 2018, August 27-29, 2018, Belgrade, Serbia = Прва међународна конференција о електронској микроскопији наноструктура ELMINA 2018, 27-29 август 2018. Београд, Србија. 2018;:240-242.
https://hdl.handle.net/21.15107/rcub_technorep_7386 .

Nešović, Katarina, Janković, Ana, Vukašinović-Sekulić, Maja, Perić-Grujić, Aleksandra, Mišković-Stanković, Vesna, "Nanocomposite Hydrogels Based on Poly(vinyl alcohol) and Chitosan with Silver Nanoparticles and Graphene Aimed for Wound Dressing Applications" in Program ; Book of Abstracts / First International Conference on Electron Microscopy of Nanostructures ELMINA 2018, August 27-29, 2018, Belgrade, Serbia = Прва међународна конференција о електронској микроскопији наноструктура ELMINA 2018, 27-29 август 2018. Београд, Србија (2018):240-242,
https://hdl.handle.net/21.15107/rcub_technorep_7386 .

TY  - JOUR
AU  - Stevanović, Milena
AU  - Đošić, Marija
AU  - Janković, Ana
AU  - Kojić, Vesna
AU  - Vukašinović-Sekulić, Maja
AU  - Stojanović, Jovica
AU  - Odović, Jadranka
AU  - Crevar-Sakač, Milkica
AU  - Rhee, Kyong Yop
AU  - Mišković-Stanković, Vesna
PY  - 2018
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3899
AB  - Composite coating of antibiotic gentamicin (Gent), natural polymer chitosan (CS), and hydroxyapatite (HAP) was successfully assessed by applying the electrophoretic deposition (EPD) technique. EPD was performed under optimized deposition conditions (5 V, 12 min) on pure titanium plates, to obtain HAP/CS and HAP/CS/Gent composite coatings in a single step from three-component aqueous suspension, with favorable antibacterial properties. Composite coatings were characterized by X-ray diffraction (XRD), field emission scanning electron microscopy, Fourier transform infrared spectroscopy, thermogravimetric analysis, and X-ray photoelectron analysis, confirming the formation of composite HAP/CS and HAP/CS/Gent coatings on the titanium surface, which is due to intermolecular hydrogen bonds. Employing the XRD technique, HAP was detected by obtaining the characteristic diffraction maximums. Good antibacterial activity of the composite coating loaded with antibiotic (HAP/CS/Gent) was confirmed against Staphylococcus aureus and Escherichia coli, pointing to the high potential for bioapplication. Introduction of gentamicin in HAP/CS/Gent coating caused very mild cytotoxicity in the tested cell lines MRC-5 and L929. MTT testing was used to evaluate cell viability, and HAP/CS was classified as noncytotoxic.
PB  - Amer Chemical Soc, Washington
T2  - ACS Biomaterials Science & Engineering
T1  - Gentamicin-Loaded Bioactive Hydroxyapatite/Chitosan Composite Coating Electrodeposited on Titanium
EP  - 4007
IS  - 12
SP  - 3994
VL  - 4
DO  - 10.1021/acsbiomaterials.8b00859
ER  - 

@article{
author = "Stevanović, Milena and Đošić, Marija and Janković, Ana and Kojić, Vesna and Vukašinović-Sekulić, Maja and Stojanović, Jovica and Odović, Jadranka and Crevar-Sakač, Milkica and Rhee, Kyong Yop and Mišković-Stanković, Vesna",
year = "2018",
abstract = "Composite coating of antibiotic gentamicin (Gent), natural polymer chitosan (CS), and hydroxyapatite (HAP) was successfully assessed by applying the electrophoretic deposition (EPD) technique. EPD was performed under optimized deposition conditions (5 V, 12 min) on pure titanium plates, to obtain HAP/CS and HAP/CS/Gent composite coatings in a single step from three-component aqueous suspension, with favorable antibacterial properties. Composite coatings were characterized by X-ray diffraction (XRD), field emission scanning electron microscopy, Fourier transform infrared spectroscopy, thermogravimetric analysis, and X-ray photoelectron analysis, confirming the formation of composite HAP/CS and HAP/CS/Gent coatings on the titanium surface, which is due to intermolecular hydrogen bonds. Employing the XRD technique, HAP was detected by obtaining the characteristic diffraction maximums. Good antibacterial activity of the composite coating loaded with antibiotic (HAP/CS/Gent) was confirmed against Staphylococcus aureus and Escherichia coli, pointing to the high potential for bioapplication. Introduction of gentamicin in HAP/CS/Gent coating caused very mild cytotoxicity in the tested cell lines MRC-5 and L929. MTT testing was used to evaluate cell viability, and HAP/CS was classified as noncytotoxic.",
publisher = "Amer Chemical Soc, Washington",
journal = "ACS Biomaterials Science & Engineering",
title = "Gentamicin-Loaded Bioactive Hydroxyapatite/Chitosan Composite Coating Electrodeposited on Titanium",
pages = "4007-3994",
number = "12",
volume = "4",
doi = "10.1021/acsbiomaterials.8b00859"
}

Stevanović, M., Đošić, M., Janković, A., Kojić, V., Vukašinović-Sekulić, M., Stojanović, J., Odović, J., Crevar-Sakač, M., Rhee, K. Y.,& Mišković-Stanković, V.. (2018). Gentamicin-Loaded Bioactive Hydroxyapatite/Chitosan Composite Coating Electrodeposited on Titanium. in ACS Biomaterials Science & Engineering
Amer Chemical Soc, Washington., 4(12), 3994-4007.
https://doi.org/10.1021/acsbiomaterials.8b00859

Stevanović M, Đošić M, Janković A, Kojić V, Vukašinović-Sekulić M, Stojanović J, Odović J, Crevar-Sakač M, Rhee KY, Mišković-Stanković V. Gentamicin-Loaded Bioactive Hydroxyapatite/Chitosan Composite Coating Electrodeposited on Titanium. in ACS Biomaterials Science & Engineering. 2018;4(12):3994-4007.
doi:10.1021/acsbiomaterials.8b00859 .

Stevanović, Milena, Đošić, Marija, Janković, Ana, Kojić, Vesna, Vukašinović-Sekulić, Maja, Stojanović, Jovica, Odović, Jadranka, Crevar-Sakač, Milkica, Rhee, Kyong Yop, Mišković-Stanković, Vesna, "Gentamicin-Loaded Bioactive Hydroxyapatite/Chitosan Composite Coating Electrodeposited on Titanium" in ACS Biomaterials Science & Engineering, 4, no. 12 (2018):3994-4007,
https://doi.org/10.1021/acsbiomaterials.8b00859 . .

TY  - JOUR
AU  - Đošić, Marija
AU  - Janković, Ana
AU  - Rhee, Kyong Yop
AU  - Mišković-Stanković, Vesna
PY  - 2018
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3820
AB  - Fast ongoing research of graphene-based nanomaterials reveals that their exceptional properties could be utized for biomedical applications, especially in tissue engineering and drug delivery. Here, we summarized the results obtained on hydroxyapatite based biocomposite coatings produced by electrophoretic deposition on titanium substrate and reinforced by nanosized graphene, as potential candidates for hard tissue implants.
AB  - Brzi razvoj istraživanja nanomaterijala koji sadrže grafen otkrio je da se njihova izuzetna svojstva mogu koristiti za biomedicinske primene, posebno u inženjerstvu tkiva i kao nosači lekova. U ovom radu su prikazani rezultati istraživanja biokompozitnih prevlaka na bazi hidroksiapatita koje su dobijene elektroforetskim taloženjem na titanskom supstratu i ojačane nanočestičnim grafenom, kao potencijalnih kandidata za implante koštanog tkiva.
PB  - Engineering Society for Corrosion, Belgrade, Serbia
T2  - Zaštita materijala
T1  - Graphene reinforced hydroxyapatite biocomposite coatings obtained by electrophoretic deposition on titanium
T1  - Biokompozitne prevlake hidroksiapatita ojačane grafenom, dobijene elektroforetskim taloženjem na titanu
EP  - 306
IS  - 2
SP  - 293
VL  - 59
DO  - 10.5937/ZasMat1802293D
ER  - 

@article{
author = "Đošić, Marija and Janković, Ana and Rhee, Kyong Yop and Mišković-Stanković, Vesna",
year = "2018",
abstract = "Fast ongoing research of graphene-based nanomaterials reveals that their exceptional properties could be utized for biomedical applications, especially in tissue engineering and drug delivery. Here, we summarized the results obtained on hydroxyapatite based biocomposite coatings produced by electrophoretic deposition on titanium substrate and reinforced by nanosized graphene, as potential candidates for hard tissue implants., Brzi razvoj istraživanja nanomaterijala koji sadrže grafen otkrio je da se njihova izuzetna svojstva mogu koristiti za biomedicinske primene, posebno u inženjerstvu tkiva i kao nosači lekova. U ovom radu su prikazani rezultati istraživanja biokompozitnih prevlaka na bazi hidroksiapatita koje su dobijene elektroforetskim taloženjem na titanskom supstratu i ojačane nanočestičnim grafenom, kao potencijalnih kandidata za implante koštanog tkiva.",
publisher = "Engineering Society for Corrosion, Belgrade, Serbia",
journal = "Zaštita materijala",
title = "Graphene reinforced hydroxyapatite biocomposite coatings obtained by electrophoretic deposition on titanium, Biokompozitne prevlake hidroksiapatita ojačane grafenom, dobijene elektroforetskim taloženjem na titanu",
pages = "306-293",
number = "2",
volume = "59",
doi = "10.5937/ZasMat1802293D"
}

Đošić, M., Janković, A., Rhee, K. Y.,& Mišković-Stanković, V.. (2018). Graphene reinforced hydroxyapatite biocomposite coatings obtained by electrophoretic deposition on titanium. in Zaštita materijala
Engineering Society for Corrosion, Belgrade, Serbia., 59(2), 293-306.
https://doi.org/10.5937/ZasMat1802293D

Đošić M, Janković A, Rhee KY, Mišković-Stanković V. Graphene reinforced hydroxyapatite biocomposite coatings obtained by electrophoretic deposition on titanium. in Zaštita materijala. 2018;59(2):293-306.
doi:10.5937/ZasMat1802293D .

Đošić, Marija, Janković, Ana, Rhee, Kyong Yop, Mišković-Stanković, Vesna, "Graphene reinforced hydroxyapatite biocomposite coatings obtained by electrophoretic deposition on titanium" in Zaštita materijala, 59, no. 2 (2018):293-306,
https://doi.org/10.5937/ZasMat1802293D . .

TY  - JOUR
AU  - Nešović, Katarina
AU  - Janković, Ana
AU  - Kojić, Vesna
AU  - Vukašinović-Sekulić, Maja
AU  - Perić-Grujić, Aleksandra
AU  - Rhee, Kyong Yop
AU  - Mišković-Stanković, Vesna
PY  - 2018
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3942
AB  - This study presents the synthesis of novel silver/poly(vinyl alcohol)/chitosan/graphene (Ag/PVA/CHI/Gr) nanocomposite hydrogels by in situ electrochemical reduction of silver ions in the hydrogel matrix and their thorough characterization by UV-visible and Raman spectroscopies, field-emission scanning electron microscopy (FE-SEM), X-ray photoelectron spectroscopy (XPS), and silver release measurements. The influence of chitosan content on the incorporation and stabilization of silver nanoparticles (AgNPs) was investigated, and we found that hydrogels with higher chitosan content contain higher amounts of AgNPs. In addition, the cytotoxicity and antibacterial activity of Ag/PVA/CHI/Gr nanocomposite hydrogels were evaluated. Based on in vitro investigations, the obtained materials exhibit diffusion-controlled release profiles over 28 days, strong antibacterial activity against Staphylococcus aureus and Escherichia coli bacterial strains, and no cytotoxicity toward human and mice fibroblast cell lines.
PB  - Elsevier Sci Ltd, Oxford
T2  - Composites Part B-Engineering
T1  - Silver/poly(vinyl alcohol)/chitosan/graphene hydrogels - Synthesis, biological and physicochemical properties and silver release kinetics
EP  - 185
SP  - 175
VL  - 154
DO  - 10.1016/j.compositesb.2018.08.005
ER  - 

@article{
author = "Nešović, Katarina and Janković, Ana and Kojić, Vesna and Vukašinović-Sekulić, Maja and Perić-Grujić, Aleksandra and Rhee, Kyong Yop and Mišković-Stanković, Vesna",
year = "2018",
abstract = "This study presents the synthesis of novel silver/poly(vinyl alcohol)/chitosan/graphene (Ag/PVA/CHI/Gr) nanocomposite hydrogels by in situ electrochemical reduction of silver ions in the hydrogel matrix and their thorough characterization by UV-visible and Raman spectroscopies, field-emission scanning electron microscopy (FE-SEM), X-ray photoelectron spectroscopy (XPS), and silver release measurements. The influence of chitosan content on the incorporation and stabilization of silver nanoparticles (AgNPs) was investigated, and we found that hydrogels with higher chitosan content contain higher amounts of AgNPs. In addition, the cytotoxicity and antibacterial activity of Ag/PVA/CHI/Gr nanocomposite hydrogels were evaluated. Based on in vitro investigations, the obtained materials exhibit diffusion-controlled release profiles over 28 days, strong antibacterial activity against Staphylococcus aureus and Escherichia coli bacterial strains, and no cytotoxicity toward human and mice fibroblast cell lines.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Composites Part B-Engineering",
title = "Silver/poly(vinyl alcohol)/chitosan/graphene hydrogels - Synthesis, biological and physicochemical properties and silver release kinetics",
pages = "185-175",
volume = "154",
doi = "10.1016/j.compositesb.2018.08.005"
}

Nešović, K., Janković, A., Kojić, V., Vukašinović-Sekulić, M., Perić-Grujić, A., Rhee, K. Y.,& Mišković-Stanković, V.. (2018). Silver/poly(vinyl alcohol)/chitosan/graphene hydrogels - Synthesis, biological and physicochemical properties and silver release kinetics. in Composites Part B-Engineering
Elsevier Sci Ltd, Oxford., 154, 175-185.
https://doi.org/10.1016/j.compositesb.2018.08.005

Nešović K, Janković A, Kojić V, Vukašinović-Sekulić M, Perić-Grujić A, Rhee KY, Mišković-Stanković V. Silver/poly(vinyl alcohol)/chitosan/graphene hydrogels - Synthesis, biological and physicochemical properties and silver release kinetics. in Composites Part B-Engineering. 2018;154:175-185.
doi:10.1016/j.compositesb.2018.08.005 .

Nešović, Katarina, Janković, Ana, Kojić, Vesna, Vukašinović-Sekulić, Maja, Perić-Grujić, Aleksandra, Rhee, Kyong Yop, Mišković-Stanković, Vesna, "Silver/poly(vinyl alcohol)/chitosan/graphene hydrogels - Synthesis, biological and physicochemical properties and silver release kinetics" in Composites Part B-Engineering, 154 (2018):175-185,
https://doi.org/10.1016/j.compositesb.2018.08.005 . .

TY  - JOUR
AU  - Đošić, Marija
AU  - Eraković, Sanja
AU  - Janković, Ana
AU  - Vukašinović-Sekulić, Maja
AU  - Matić, Ivana Z.
AU  - Stojanović, Jovica
AU  - Rhee, Kyong Yop
AU  - Mišković-Stanković, Vesna
AU  - Park, Soo-Jin
PY  - 2017
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3703
AB  - Graphene (Gr) and natural polymer chitosan (CS) were introduced to hydroxyapatite (HAP) to produce a three-component composite coating, which was fabricated by cathodic electrophoretic deposition on Ti substrates in an ethanol suspension. These HAP/CS/Gr coatings were characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), thermogravimetric analysis (TGA), X-ray photoelectron spectroscopy (XPS) and electrochemical measurements and found that the graphene into HAP/CS composites significantly improves their morphology, thermal stability, and bioactivity. Both HAP/CS and HAP/CS/Gr composite coatings are classified as non-cytotoxic when tested against healthy peripheral blood mononuclear cells (PBMC), while antibacterial activity against Staphylococcus aureus and Escherichia coil could not be verified.
PB  - Elsevier Science Inc, New York
T2  - Journal of Industrial and Engineering Chemistry
T1  - In vitro investigation of electrophoretically deposited bioactive hydroxyapatite/chitosan coatings reinforced by graphene
EP  - 347
SP  - 336
VL  - 47
DO  - 10.1016/j.jiec.2016.12.004
ER  - 

@article{
author = "Đošić, Marija and Eraković, Sanja and Janković, Ana and Vukašinović-Sekulić, Maja and Matić, Ivana Z. and Stojanović, Jovica and Rhee, Kyong Yop and Mišković-Stanković, Vesna and Park, Soo-Jin",
year = "2017",
abstract = "Graphene (Gr) and natural polymer chitosan (CS) were introduced to hydroxyapatite (HAP) to produce a three-component composite coating, which was fabricated by cathodic electrophoretic deposition on Ti substrates in an ethanol suspension. These HAP/CS/Gr coatings were characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), thermogravimetric analysis (TGA), X-ray photoelectron spectroscopy (XPS) and electrochemical measurements and found that the graphene into HAP/CS composites significantly improves their morphology, thermal stability, and bioactivity. Both HAP/CS and HAP/CS/Gr composite coatings are classified as non-cytotoxic when tested against healthy peripheral blood mononuclear cells (PBMC), while antibacterial activity against Staphylococcus aureus and Escherichia coil could not be verified.",
publisher = "Elsevier Science Inc, New York",
journal = "Journal of Industrial and Engineering Chemistry",
title = "In vitro investigation of electrophoretically deposited bioactive hydroxyapatite/chitosan coatings reinforced by graphene",
pages = "347-336",
volume = "47",
doi = "10.1016/j.jiec.2016.12.004"
}

Đošić, M., Eraković, S., Janković, A., Vukašinović-Sekulić, M., Matić, I. Z., Stojanović, J., Rhee, K. Y., Mišković-Stanković, V.,& Park, S.. (2017). In vitro investigation of electrophoretically deposited bioactive hydroxyapatite/chitosan coatings reinforced by graphene. in Journal of Industrial and Engineering Chemistry
Elsevier Science Inc, New York., 47, 336-347.
https://doi.org/10.1016/j.jiec.2016.12.004

Đošić M, Eraković S, Janković A, Vukašinović-Sekulić M, Matić IZ, Stojanović J, Rhee KY, Mišković-Stanković V, Park S. In vitro investigation of electrophoretically deposited bioactive hydroxyapatite/chitosan coatings reinforced by graphene. in Journal of Industrial and Engineering Chemistry. 2017;47:336-347.
doi:10.1016/j.jiec.2016.12.004 .

Đošić, Marija, Eraković, Sanja, Janković, Ana, Vukašinović-Sekulić, Maja, Matić, Ivana Z., Stojanović, Jovica, Rhee, Kyong Yop, Mišković-Stanković, Vesna, Park, Soo-Jin, "In vitro investigation of electrophoretically deposited bioactive hydroxyapatite/chitosan coatings reinforced by graphene" in Journal of Industrial and Engineering Chemistry, 47 (2017):336-347,
https://doi.org/10.1016/j.jiec.2016.12.004 . .

TY  - JOUR
AU  - Surudžić, Rade
AU  - Janković, Ana
AU  - Vukašinović-Sekulić, Maja
AU  - Perić-Grujić, Aleksandra
AU  - Rhee, Kyong Yop
AU  - Mišković-Stanković, Vesna
PY  - 2017
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3594
AB  - This work focuses on the optimization of electrochemical synthesis of silver nanoparticles in poly(vinyl alcohol) colloid solutions, films and hydrogel discs with the ability for immobilization of silver nanoparticles and their progressive release, as a new, prospective generation of medical wound dressings. Incorporation of silver nanoparticles into polyvinyl alcohol and polyvinyl alcohol/graphene matrices, with the aim to determine the effect of silver and graphene, was achieved by the electrochemical synthesis at different values of constant current density between 10 and 40 mA cm(-2) and for different time between 10 and 75 min, followed by drying (to obtain films) or freezing-thawing method (to obtain hydrogels). Synthesized nanocomposites were characterized by UV-Visible spectroscopy (UV-Vis), transmission electron microscopy (TEM), cyclic voltammetry (CV), Fourier transform infrared spectroscopy (FT-IR), Raman spectroscopy and atomic absorption spectroscopy (AAS). Additionally, antibacterial activity of thus prepared samples was evaluated against pathogenic bacteria strains Staphylococcus aureus and Escherichia coli using agar diffusion test. The results indicated that both silver/polyvinyl alcohol and silver polyvinyl alcohol/graphene nanocomposites are excellent candidates for soft tissue implants and wound dressings.
PB  - Bulgarian Acad Science, Sofia
T2  - Bulgarian Chemical Communications
T1  - Optimization of the electrochemical synthesis of silver nanoparticles in poly(vinyl alcohol) colloid solutions
EP  - 193
SP  - 186
VL  - 49
UR  - https://hdl.handle.net/21.15107/rcub_technorep_3594
ER  - 

@article{
author = "Surudžić, Rade and Janković, Ana and Vukašinović-Sekulić, Maja and Perić-Grujić, Aleksandra and Rhee, Kyong Yop and Mišković-Stanković, Vesna",
year = "2017",
abstract = "This work focuses on the optimization of electrochemical synthesis of silver nanoparticles in poly(vinyl alcohol) colloid solutions, films and hydrogel discs with the ability for immobilization of silver nanoparticles and their progressive release, as a new, prospective generation of medical wound dressings. Incorporation of silver nanoparticles into polyvinyl alcohol and polyvinyl alcohol/graphene matrices, with the aim to determine the effect of silver and graphene, was achieved by the electrochemical synthesis at different values of constant current density between 10 and 40 mA cm(-2) and for different time between 10 and 75 min, followed by drying (to obtain films) or freezing-thawing method (to obtain hydrogels). Synthesized nanocomposites were characterized by UV-Visible spectroscopy (UV-Vis), transmission electron microscopy (TEM), cyclic voltammetry (CV), Fourier transform infrared spectroscopy (FT-IR), Raman spectroscopy and atomic absorption spectroscopy (AAS). Additionally, antibacterial activity of thus prepared samples was evaluated against pathogenic bacteria strains Staphylococcus aureus and Escherichia coli using agar diffusion test. The results indicated that both silver/polyvinyl alcohol and silver polyvinyl alcohol/graphene nanocomposites are excellent candidates for soft tissue implants and wound dressings.",
publisher = "Bulgarian Acad Science, Sofia",
journal = "Bulgarian Chemical Communications",
title = "Optimization of the electrochemical synthesis of silver nanoparticles in poly(vinyl alcohol) colloid solutions",
pages = "193-186",
volume = "49",
url = "https://hdl.handle.net/21.15107/rcub_technorep_3594"
}

Surudžić, R., Janković, A., Vukašinović-Sekulić, M., Perić-Grujić, A., Rhee, K. Y.,& Mišković-Stanković, V.. (2017). Optimization of the electrochemical synthesis of silver nanoparticles in poly(vinyl alcohol) colloid solutions. in Bulgarian Chemical Communications
Bulgarian Acad Science, Sofia., 49, 186-193.
https://hdl.handle.net/21.15107/rcub_technorep_3594

Surudžić R, Janković A, Vukašinović-Sekulić M, Perić-Grujić A, Rhee KY, Mišković-Stanković V. Optimization of the electrochemical synthesis of silver nanoparticles in poly(vinyl alcohol) colloid solutions. in Bulgarian Chemical Communications. 2017;49:186-193.
https://hdl.handle.net/21.15107/rcub_technorep_3594 .

Surudžić, Rade, Janković, Ana, Vukašinović-Sekulić, Maja, Perić-Grujić, Aleksandra, Rhee, Kyong Yop, Mišković-Stanković, Vesna, "Optimization of the electrochemical synthesis of silver nanoparticles in poly(vinyl alcohol) colloid solutions" in Bulgarian Chemical Communications, 49 (2017):186-193,
https://hdl.handle.net/21.15107/rcub_technorep_3594 .