TY  - JOUR
AU  - Lunić, Tanja
AU  - Lađarević, Jelena
AU  - Mandić, Marija
AU  - Veruševski, Vanja
AU  - Božić-Nedeljković, Biljana
AU  - Mijin, Dušan
AU  - Božić, Bojan
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4728
AB  - Series of twenty-four 2-pyridone derivatives were screened and selected using the antioxidant ABTS assay
in order to find the most prominent compounds for further in vitro and in silico analyses. Seven derivatives with the highest antioxidant activities were selected and evaluated by β-carotene bleaching assay,
where all of the compounds exhibited better activities than standard antioxidant substance, vitamin C.
Furthermore, seven selected 2-pyridones exhibited significant anti-neuroinflammatory activity by reducing the production of pro-inflammatory mediators (ROS, NO, IL-6, and TNF-α) by LPS-stimulated BV2
microglial cells. Moreover, neuroprotective activity in microglia supernatant transfer model system on
SH-SY5Y neuronal cells has been observed. Anti-neuroinflammatory mechanisms of selected 2-pyridones
have been investigated by molecular docking studies of the TLR4 signaling pathway proteins as well as
inducible nitric oxide synthase. The present study suggests that selected 2-pyridone derivatives can alleviate neuroinflammation in microglial cells and protect the surrounding neuronal cells from damage,
which qualifies them for further investigations towards neurodegenerative diseases associated with inflammation.
PB  - Elsevier B.V.
T2  - Journal of Molecular Structure
T1  - Antioxidant and neuroprotective activities of selected 2-pyridones: In vitro and in silico study
SP  - 132546
VL  - 1256
DO  - 10.1016/j.molstruc.2022.132546
ER  - 

@article{
author = "Lunić, Tanja and Lađarević, Jelena and Mandić, Marija and Veruševski, Vanja and Božić-Nedeljković, Biljana and Mijin, Dušan and Božić, Bojan",
year = "2022",
abstract = "Series of twenty-four 2-pyridone derivatives were screened and selected using the antioxidant ABTS assay
in order to find the most prominent compounds for further in vitro and in silico analyses. Seven derivatives with the highest antioxidant activities were selected and evaluated by β-carotene bleaching assay,
where all of the compounds exhibited better activities than standard antioxidant substance, vitamin C.
Furthermore, seven selected 2-pyridones exhibited significant anti-neuroinflammatory activity by reducing the production of pro-inflammatory mediators (ROS, NO, IL-6, and TNF-α) by LPS-stimulated BV2
microglial cells. Moreover, neuroprotective activity in microglia supernatant transfer model system on
SH-SY5Y neuronal cells has been observed. Anti-neuroinflammatory mechanisms of selected 2-pyridones
have been investigated by molecular docking studies of the TLR4 signaling pathway proteins as well as
inducible nitric oxide synthase. The present study suggests that selected 2-pyridone derivatives can alleviate neuroinflammation in microglial cells and protect the surrounding neuronal cells from damage,
which qualifies them for further investigations towards neurodegenerative diseases associated with inflammation.",
publisher = "Elsevier B.V.",
journal = "Journal of Molecular Structure",
title = "Antioxidant and neuroprotective activities of selected 2-pyridones: In vitro and in silico study",
pages = "132546",
volume = "1256",
doi = "10.1016/j.molstruc.2022.132546"
}

Lunić, T., Lađarević, J., Mandić, M., Veruševski, V., Božić-Nedeljković, B., Mijin, D.,& Božić, B.. (2022). Antioxidant and neuroprotective activities of selected 2-pyridones: In vitro and in silico study. in Journal of Molecular Structure
Elsevier B.V.., 1256, 132546.
https://doi.org/10.1016/j.molstruc.2022.132546

Lunić T, Lađarević J, Mandić M, Veruševski V, Božić-Nedeljković B, Mijin D, Božić B. Antioxidant and neuroprotective activities of selected 2-pyridones: In vitro and in silico study. in Journal of Molecular Structure. 2022;1256:132546.
doi:10.1016/j.molstruc.2022.132546 .

Lunić, Tanja, Lađarević, Jelena, Mandić, Marija, Veruševski, Vanja, Božić-Nedeljković, Biljana, Mijin, Dušan, Božić, Bojan, "Antioxidant and neuroprotective activities of selected 2-pyridones: In vitro and in silico study" in Journal of Molecular Structure, 1256 (2022):132546,
https://doi.org/10.1016/j.molstruc.2022.132546 . .

TY  - JOUR
AU  - Vuković, Jovana
AU  - Perić-Grujić, Aleksandra
AU  - Mitić-Culafić, Dragana S.
AU  - Božić-Nedeljković, Biljana
AU  - Tomić, Simonida
PY  - 2020
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4345
AB  - Since the management of infections becomes prior global healthcare issue, the "post antibiotic era" requires innovative and interdisciplinary approach. As an alternative to widespread and, nowdays mostly uneffective, antibiotic treatment of infections, the series of hydrogels were developed and further investigated as novel antibacterial biomaterials. The hydrogels based on 2-hydroxyethyl acrylate and itaconic acid were synthesized and used for silver(I) ions incorporation. The structural, thermal and swelling characteristics were examined by Fourier transform infrared spectroscopy, differential scanning calorimetry, and swelling study conducted in wide range of pHs at 37 degrees C. Results confirmed the expected structure, while the glass transition temperatures (T-g) of the hydrogels were detected in range of 10-37 degrees C. The in vitro release study revealed suitability of these pH sensitive hydrogels as the systems for topical delivery of silver(I) ions. Performed MTT test and Comet assay proved biocompatibility of the hydrogels, as well as the absence of acute genotoxic effect on human fibroblast cells (MRC-5). The hydrogels exhibited satisfying antibacterial activity against methicillin sensitive Staphylococcus aureus (MSSA) and methicillin resistant Staphylococcus aureus (MRSA), indicating the capacity to treat the life-threatening infections.
PB  - Polymer Soc Korea, Seoul
T2  - Macromolecular Research
T1  - Antibacterial Activity of pH-Sensitive Silver(I)/Poly(2-hydroxyethyl acrylate/itaconic acid) Hydrogels
EP  - 389
IS  - 4
SP  - 382
VL  - 28
DO  - 10.1007/s13233-020-8050-z
ER  - 

@article{
author = "Vuković, Jovana and Perić-Grujić, Aleksandra and Mitić-Culafić, Dragana S. and Božić-Nedeljković, Biljana and Tomić, Simonida",
year = "2020",
abstract = "Since the management of infections becomes prior global healthcare issue, the "post antibiotic era" requires innovative and interdisciplinary approach. As an alternative to widespread and, nowdays mostly uneffective, antibiotic treatment of infections, the series of hydrogels were developed and further investigated as novel antibacterial biomaterials. The hydrogels based on 2-hydroxyethyl acrylate and itaconic acid were synthesized and used for silver(I) ions incorporation. The structural, thermal and swelling characteristics were examined by Fourier transform infrared spectroscopy, differential scanning calorimetry, and swelling study conducted in wide range of pHs at 37 degrees C. Results confirmed the expected structure, while the glass transition temperatures (T-g) of the hydrogels were detected in range of 10-37 degrees C. The in vitro release study revealed suitability of these pH sensitive hydrogels as the systems for topical delivery of silver(I) ions. Performed MTT test and Comet assay proved biocompatibility of the hydrogels, as well as the absence of acute genotoxic effect on human fibroblast cells (MRC-5). The hydrogels exhibited satisfying antibacterial activity against methicillin sensitive Staphylococcus aureus (MSSA) and methicillin resistant Staphylococcus aureus (MRSA), indicating the capacity to treat the life-threatening infections.",
publisher = "Polymer Soc Korea, Seoul",
journal = "Macromolecular Research",
title = "Antibacterial Activity of pH-Sensitive Silver(I)/Poly(2-hydroxyethyl acrylate/itaconic acid) Hydrogels",
pages = "389-382",
number = "4",
volume = "28",
doi = "10.1007/s13233-020-8050-z"
}

Vuković, J., Perić-Grujić, A., Mitić-Culafić, D. S., Božić-Nedeljković, B.,& Tomić, S.. (2020). Antibacterial Activity of pH-Sensitive Silver(I)/Poly(2-hydroxyethyl acrylate/itaconic acid) Hydrogels. in Macromolecular Research
Polymer Soc Korea, Seoul., 28(4), 382-389.
https://doi.org/10.1007/s13233-020-8050-z

Vuković J, Perić-Grujić A, Mitić-Culafić DS, Božić-Nedeljković B, Tomić S. Antibacterial Activity of pH-Sensitive Silver(I)/Poly(2-hydroxyethyl acrylate/itaconic acid) Hydrogels. in Macromolecular Research. 2020;28(4):382-389.
doi:10.1007/s13233-020-8050-z .

Vuković, Jovana, Perić-Grujić, Aleksandra, Mitić-Culafić, Dragana S., Božić-Nedeljković, Biljana, Tomić, Simonida, "Antibacterial Activity of pH-Sensitive Silver(I)/Poly(2-hydroxyethyl acrylate/itaconic acid) Hydrogels" in Macromolecular Research, 28, no. 4 (2020):382-389,
https://doi.org/10.1007/s13233-020-8050-z . .

TY  - JOUR
AU  - Lađarević, Jelena
AU  - Božić, Bojan
AU  - Matović, Luka
AU  - Božić-Nedeljković, Biljana
AU  - Mijin, Dušan
PY  - 2019
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4312
AB  - A series of novel 5-(4-substituted phenylazo)-1-carboxymethyl-3-cyano-6-hydroxy-4-methyl-2-pyridones had been synthesized and characterized. The study includes spectral determination of the possible tautomeric forms and the intramolecular hydrogen bonding pattern. The influence of the bifurcated intramolecular hydrogen bond on the spectral properties has been discussed. The dyes exist in hydrazone form both solid state and in most of the employed solvents. Equilibrium between hydrazone and anion forms has been studied in N,N-dimethylformamide and N,N-dimethylacetamide. For quantitative elucidation of solvent effects on UV-Vis absorption maxima, a linear solvation energy relationship concept has been employed using Kamlet-Taft's and Catalan's equations. Antioxidative activity of the synthesized dyes has been chemically tested and two derivatives (hydroxy and methoxy) are qualified as promising antioxidant molecules.
PB  - Elsevier Sci Ltd, Oxford
T2  - Dyes and Pigments
T1  - Role of the bifurcated intramolecular hydrogen bond on the physicochemical profile of the novel azo pyridone dyes
EP  - 572
SP  - 562
VL  - 162
DO  - 10.1016/j.dyepig.2018.10.058
ER  - 

@article{
author = "Lađarević, Jelena and Božić, Bojan and Matović, Luka and Božić-Nedeljković, Biljana and Mijin, Dušan",
year = "2019",
abstract = "A series of novel 5-(4-substituted phenylazo)-1-carboxymethyl-3-cyano-6-hydroxy-4-methyl-2-pyridones had been synthesized and characterized. The study includes spectral determination of the possible tautomeric forms and the intramolecular hydrogen bonding pattern. The influence of the bifurcated intramolecular hydrogen bond on the spectral properties has been discussed. The dyes exist in hydrazone form both solid state and in most of the employed solvents. Equilibrium between hydrazone and anion forms has been studied in N,N-dimethylformamide and N,N-dimethylacetamide. For quantitative elucidation of solvent effects on UV-Vis absorption maxima, a linear solvation energy relationship concept has been employed using Kamlet-Taft's and Catalan's equations. Antioxidative activity of the synthesized dyes has been chemically tested and two derivatives (hydroxy and methoxy) are qualified as promising antioxidant molecules.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Dyes and Pigments",
title = "Role of the bifurcated intramolecular hydrogen bond on the physicochemical profile of the novel azo pyridone dyes",
pages = "572-562",
volume = "162",
doi = "10.1016/j.dyepig.2018.10.058"
}

Lađarević, J., Božić, B., Matović, L., Božić-Nedeljković, B.,& Mijin, D.. (2019). Role of the bifurcated intramolecular hydrogen bond on the physicochemical profile of the novel azo pyridone dyes. in Dyes and Pigments
Elsevier Sci Ltd, Oxford., 162, 562-572.
https://doi.org/10.1016/j.dyepig.2018.10.058

Lađarević J, Božić B, Matović L, Božić-Nedeljković B, Mijin D. Role of the bifurcated intramolecular hydrogen bond on the physicochemical profile of the novel azo pyridone dyes. in Dyes and Pigments. 2019;162:562-572.
doi:10.1016/j.dyepig.2018.10.058 .

Lađarević, Jelena, Božić, Bojan, Matović, Luka, Božić-Nedeljković, Biljana, Mijin, Dušan, "Role of the bifurcated intramolecular hydrogen bond on the physicochemical profile of the novel azo pyridone dyes" in Dyes and Pigments, 162 (2019):562-572,
https://doi.org/10.1016/j.dyepig.2018.10.058 . .

TY  - JOUR
AU  - Lazić, Anita M.
AU  - Radovanović, Lidija D.
AU  - Božić, Bojan Đ.
AU  - Božić Nedeljković, Biljana Đ.
AU  - Vitnik, Vesna D.
AU  - Vitnik, Željko J.
AU  - Rogan, Jelena R.
AU  - Valentić, Nataša V.
AU  - Ušćumlić, Gordana S.
AU  - Trišović, Nemanja P.
PY  - 2019
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4324
AB  - Two series of cycloalkanespiro-5-hydantoins, namely cyclo-hexanespiro-5-hydantoins and cycloheptanespiro-5-hydantoins with a 4-substituted benzyl or a 2-(4-substituted phenyl)-2-oxoethyl group at N3 position, were synthesized and their effects on proliferation of human colon (HCT-116), leukemia (K562) and breast (MDA-MB-231) cancer cell lines were tested. For comparison, we also described the 5,5-diphenylhydantoin analogues. The structural features of the investigated compounds were characterized by elemental analysis, FT-IR, UV-Vis, H-1 and C-13 NMR spectroscopy and X-ray crystallography. Regarding their structure-activity relationships, it was shown that the substitution on the benzyl moiety with the methoxy, chloro or bromo group potentiated the antiproliferative activity relative to the parent compounds, while an increase in the size of the cycloalkyl group resulted mostly in a decrease of the antiproliferative activity. The single crystal X-ray analysis revealed the existence of dimers and chains formed by the N-H center dot center dot center dot center dot O hydrogen bonds. The analysis of the molecular descriptors of Lipinski demonstrated that all investigated compounds obeyed the rule of five. To further understand their geometry and electronic structure, OFT calculations with B3LYP method using 6-311++G(d,p) basic set were performed. In this context, the UV-Vis spectra of the investigated compounds were analyzed in detail, whereby the predicted absorption spectra from DFT calculation matched the experimentally obtained ones, with a good correlation. The interesting physico-chemical and pharmacologically relevant properties of the investigated compounds warrant their further investigation.
PB  - Elsevier Science Bv, Amsterdam
T2  - Journal of Molecular Structure
T1  - Synthesis, structural characterization, DFT calculations and antiproliferative evaluation of novel spirohydantoin derivatives containing a substituted benzyl moiety
EP  - 62
SP  - 48
VL  - 1180
DO  - 10.1016/j.molstruc.2018.11.071
ER  - 

@article{
author = "Lazić, Anita M. and Radovanović, Lidija D. and Božić, Bojan Đ. and Božić Nedeljković, Biljana Đ. and Vitnik, Vesna D. and Vitnik, Željko J. and Rogan, Jelena R. and Valentić, Nataša V. and Ušćumlić, Gordana S. and Trišović, Nemanja P.",
year = "2019",
abstract = "Two series of cycloalkanespiro-5-hydantoins, namely cyclo-hexanespiro-5-hydantoins and cycloheptanespiro-5-hydantoins with a 4-substituted benzyl or a 2-(4-substituted phenyl)-2-oxoethyl group at N3 position, were synthesized and their effects on proliferation of human colon (HCT-116), leukemia (K562) and breast (MDA-MB-231) cancer cell lines were tested. For comparison, we also described the 5,5-diphenylhydantoin analogues. The structural features of the investigated compounds were characterized by elemental analysis, FT-IR, UV-Vis, H-1 and C-13 NMR spectroscopy and X-ray crystallography. Regarding their structure-activity relationships, it was shown that the substitution on the benzyl moiety with the methoxy, chloro or bromo group potentiated the antiproliferative activity relative to the parent compounds, while an increase in the size of the cycloalkyl group resulted mostly in a decrease of the antiproliferative activity. The single crystal X-ray analysis revealed the existence of dimers and chains formed by the N-H center dot center dot center dot center dot O hydrogen bonds. The analysis of the molecular descriptors of Lipinski demonstrated that all investigated compounds obeyed the rule of five. To further understand their geometry and electronic structure, OFT calculations with B3LYP method using 6-311++G(d,p) basic set were performed. In this context, the UV-Vis spectra of the investigated compounds were analyzed in detail, whereby the predicted absorption spectra from DFT calculation matched the experimentally obtained ones, with a good correlation. The interesting physico-chemical and pharmacologically relevant properties of the investigated compounds warrant their further investigation.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Journal of Molecular Structure",
title = "Synthesis, structural characterization, DFT calculations and antiproliferative evaluation of novel spirohydantoin derivatives containing a substituted benzyl moiety",
pages = "62-48",
volume = "1180",
doi = "10.1016/j.molstruc.2018.11.071"
}

Lazić, A. M., Radovanović, L. D., Božić, B. Đ., Božić Nedeljković, B. Đ., Vitnik, V. D., Vitnik, Ž. J., Rogan, J. R., Valentić, N. V., Ušćumlić, G. S.,& Trišović, N. P.. (2019). Synthesis, structural characterization, DFT calculations and antiproliferative evaluation of novel spirohydantoin derivatives containing a substituted benzyl moiety. in Journal of Molecular Structure
Elsevier Science Bv, Amsterdam., 1180, 48-62.
https://doi.org/10.1016/j.molstruc.2018.11.071

Lazić AM, Radovanović LD, Božić BĐ, Božić Nedeljković BĐ, Vitnik VD, Vitnik ŽJ, Rogan JR, Valentić NV, Ušćumlić GS, Trišović NP. Synthesis, structural characterization, DFT calculations and antiproliferative evaluation of novel spirohydantoin derivatives containing a substituted benzyl moiety. in Journal of Molecular Structure. 2019;1180:48-62.
doi:10.1016/j.molstruc.2018.11.071 .

Lazić, Anita M., Radovanović, Lidija D., Božić, Bojan Đ., Božić Nedeljković, Biljana Đ., Vitnik, Vesna D., Vitnik, Željko J., Rogan, Jelena R., Valentić, Nataša V., Ušćumlić, Gordana S., Trišović, Nemanja P., "Synthesis, structural characterization, DFT calculations and antiproliferative evaluation of novel spirohydantoin derivatives containing a substituted benzyl moiety" in Journal of Molecular Structure, 1180 (2019):48-62,
https://doi.org/10.1016/j.molstruc.2018.11.071 . .

TY  - JOUR
AU  - Tot, Kristina
AU  - Lazić, Anita
AU  - Božić, Biljana
AU  - Mandić, Anamarija
AU  - Đaković-Sekulić, Tatjana
PY  - 2019
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4111
AB  - Hydantois have been identified as constituents of a number of pharmacologically active molecules. In the present study, we have examined in vitro antiproliferative activity against human colon cancer cell lines HCT-116 of three series of 3-(4-substituted benzyl)-hydantoins with various substituent attached in position 5 of the hydantoin ring. Since the investigated compounds have recently been synthesized and show antiproliferative activity, a good understanding of the properties of the potential drug responsible for their pharmacokinetics is an important goal for their further development. One of the important properties is lipophilicity. Lipophilicity has been assessed by reversed-phase liquid chromatography (high-performance thin-layer chromatography and high-pressure liquid chromatography) by means of direct and indirect (using calibration curve) methods. Chromatographic lipophilicity indices in addition to calculated logP values were compared by hierarchical cluster analysis. The linear solvation energy relationship approach was used to understand and compare the types and relative strength of the molecular interactions that occur in the chromatographic as well as in the n-octanol-water partitioning systems. Finally, correlation between in silico pharmacokinetic predictors and antiproliferative activity was examined. Preliminary quantitative structure-activity relationship modeling indicates that pharmacokinetic predictors capture only one-quarter of all chemical features that are important for antiproliferative activity itself. Among selected descriptors are chromatographic lipophilicity indices.
PB  - Wiley, Hoboken
T2  - Biomedical Chromatography
T1  - QSAR characterization of new synthesized hydantoins with antiproliferative activity
IS  - 8
VL  - 33
DO  - 10.1002/bmc.4539
ER  - 

@article{
author = "Tot, Kristina and Lazić, Anita and Božić, Biljana and Mandić, Anamarija and Đaković-Sekulić, Tatjana",
year = "2019",
abstract = "Hydantois have been identified as constituents of a number of pharmacologically active molecules. In the present study, we have examined in vitro antiproliferative activity against human colon cancer cell lines HCT-116 of three series of 3-(4-substituted benzyl)-hydantoins with various substituent attached in position 5 of the hydantoin ring. Since the investigated compounds have recently been synthesized and show antiproliferative activity, a good understanding of the properties of the potential drug responsible for their pharmacokinetics is an important goal for their further development. One of the important properties is lipophilicity. Lipophilicity has been assessed by reversed-phase liquid chromatography (high-performance thin-layer chromatography and high-pressure liquid chromatography) by means of direct and indirect (using calibration curve) methods. Chromatographic lipophilicity indices in addition to calculated logP values were compared by hierarchical cluster analysis. The linear solvation energy relationship approach was used to understand and compare the types and relative strength of the molecular interactions that occur in the chromatographic as well as in the n-octanol-water partitioning systems. Finally, correlation between in silico pharmacokinetic predictors and antiproliferative activity was examined. Preliminary quantitative structure-activity relationship modeling indicates that pharmacokinetic predictors capture only one-quarter of all chemical features that are important for antiproliferative activity itself. Among selected descriptors are chromatographic lipophilicity indices.",
publisher = "Wiley, Hoboken",
journal = "Biomedical Chromatography",
title = "QSAR characterization of new synthesized hydantoins with antiproliferative activity",
number = "8",
volume = "33",
doi = "10.1002/bmc.4539"
}

Tot, K., Lazić, A., Božić, B., Mandić, A.,& Đaković-Sekulić, T.. (2019). QSAR characterization of new synthesized hydantoins with antiproliferative activity. in Biomedical Chromatography
Wiley, Hoboken., 33(8).
https://doi.org/10.1002/bmc.4539

Tot K, Lazić A, Božić B, Mandić A, Đaković-Sekulić T. QSAR characterization of new synthesized hydantoins with antiproliferative activity. in Biomedical Chromatography. 2019;33(8).
doi:10.1002/bmc.4539 .

Tot, Kristina, Lazić, Anita, Božić, Biljana, Mandić, Anamarija, Đaković-Sekulić, Tatjana, "QSAR characterization of new synthesized hydantoins with antiproliferative activity" in Biomedical Chromatography, 33, no. 8 (2019),
https://doi.org/10.1002/bmc.4539 . .

TY  - JOUR
AU  - Babić, Marija
AU  - Božić, Bojan
AU  - Božić, Biljana
AU  - Ušćumlić, Gordana
AU  - Tomić, Simonida
PY  - 2018
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3979
AB  - The discovery of novel biodegradable biomaterials able to support and control cellular activity as well as development of an enhanced and efficient method for their fabrication, are of paramount importance in the field of tissue engineering. This study highlights the design of novel degradable hydrogels based on gelatin and hydroxyethyl (meth)acrylates using the innovative combined two-step sequential microwave-assisted and UV photo-polymerization technique. Chemical composition, morphology, swelling capacity and degradation rate of the synthesized hydrogels were evaluated by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), swelling and weight loss measurements. As an initial step for evaluation of performance of the hydrogels in the biological environment, the in vitro biocompatibility of these hydrogels, was evaluated using L929 mouse fibroblasts. Obtained results demonstrated that the hydrogels possess a porous morphology with interconnected pores, 50% in vitro degradation after 7 months, and satisfied biocompatibility on L929 fibroblast cells. These unique performances of the hydrogels make them promising candidates for in vivo evaluation in clinical studies aiming at tissue regeneration.
PB  - Elsevier Science Bv, Amsterdam
T2  - Materials Letters
T1  - The innovative combined microwave-assisted and photo-polymerization technique for synthesis of the novel degradable hydroxyethyl (meth)acrylate/gelatin based scaffolds
EP  - 240
SP  - 236
VL  - 213
DO  - 10.1016/j.matlet.2017.11.087
ER  - 

@article{
author = "Babić, Marija and Božić, Bojan and Božić, Biljana and Ušćumlić, Gordana and Tomić, Simonida",
year = "2018",
abstract = "The discovery of novel biodegradable biomaterials able to support and control cellular activity as well as development of an enhanced and efficient method for their fabrication, are of paramount importance in the field of tissue engineering. This study highlights the design of novel degradable hydrogels based on gelatin and hydroxyethyl (meth)acrylates using the innovative combined two-step sequential microwave-assisted and UV photo-polymerization technique. Chemical composition, morphology, swelling capacity and degradation rate of the synthesized hydrogels were evaluated by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), swelling and weight loss measurements. As an initial step for evaluation of performance of the hydrogels in the biological environment, the in vitro biocompatibility of these hydrogels, was evaluated using L929 mouse fibroblasts. Obtained results demonstrated that the hydrogels possess a porous morphology with interconnected pores, 50% in vitro degradation after 7 months, and satisfied biocompatibility on L929 fibroblast cells. These unique performances of the hydrogels make them promising candidates for in vivo evaluation in clinical studies aiming at tissue regeneration.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Materials Letters",
title = "The innovative combined microwave-assisted and photo-polymerization technique for synthesis of the novel degradable hydroxyethyl (meth)acrylate/gelatin based scaffolds",
pages = "240-236",
volume = "213",
doi = "10.1016/j.matlet.2017.11.087"
}

Babić, M., Božić, B., Božić, B., Ušćumlić, G.,& Tomić, S.. (2018). The innovative combined microwave-assisted and photo-polymerization technique for synthesis of the novel degradable hydroxyethyl (meth)acrylate/gelatin based scaffolds. in Materials Letters
Elsevier Science Bv, Amsterdam., 213, 236-240.
https://doi.org/10.1016/j.matlet.2017.11.087

Babić M, Božić B, Božić B, Ušćumlić G, Tomić S. The innovative combined microwave-assisted and photo-polymerization technique for synthesis of the novel degradable hydroxyethyl (meth)acrylate/gelatin based scaffolds. in Materials Letters. 2018;213:236-240.
doi:10.1016/j.matlet.2017.11.087 .

Babić, Marija, Božić, Bojan, Božić, Biljana, Ušćumlić, Gordana, Tomić, Simonida, "The innovative combined microwave-assisted and photo-polymerization technique for synthesis of the novel degradable hydroxyethyl (meth)acrylate/gelatin based scaffolds" in Materials Letters, 213 (2018):236-240,
https://doi.org/10.1016/j.matlet.2017.11.087 . .

TY  - JOUR
AU  - Mijin, Dušan
AU  - Božić-Nedeljković, Biljana
AU  - Božić, Bojan
AU  - Kovrlija, Ilijana
AU  - Lađarević, Jelena
AU  - Ušćumlić, Gordana
PY  - 2018
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4008
AB  - New azo dyes bearing 2-pyridone and benzimidazole moieties were prepared using diazotization of 4-(1H-benzo[d]imidazol-2-yl)aniline and coupling of the obtained diazonium salt with substituted 3-cyano-2-pyridones. Obtained compounds were characterized via UV-Vis, FT-IR, and H-1 and C-13 NMR spectroscopy as well as by elemental analysis data. The UV-Vis spectra of the synthesized dyes were measured in thirteen solvents of different properties at room temperature. Solvatochromism and tautomerism of novel azo dyes were discussed. An MTT (3,4,5-dimethyl-thiazol-2-yl)-2,5-diphenyl tetrazolium bromide) test was performed to prove the biocompatibility of the investigated dyes. The investigated dyes exhibited satisfying antiproliferative activities against both tumor cell lines, MDA-MB-231 and HCT-116, demonstrating the potent capacity for treatment of tumors.
PB  - Scientific Technical Research Council Turkey-Tubitak, Ankara
T2  - Turkish Journal of Chemistry
T1  - Synthesis, solvatochromism, and biological activity of novel azo dyes bearing 2-pyridone and benzimidazole moieties
EP  - 907
IS  - 3
SP  - 896
VL  - 42
DO  - 10.3906/kim-1711-97
ER  - 

@article{
author = "Mijin, Dušan and Božić-Nedeljković, Biljana and Božić, Bojan and Kovrlija, Ilijana and Lađarević, Jelena and Ušćumlić, Gordana",
year = "2018",
abstract = "New azo dyes bearing 2-pyridone and benzimidazole moieties were prepared using diazotization of 4-(1H-benzo[d]imidazol-2-yl)aniline and coupling of the obtained diazonium salt with substituted 3-cyano-2-pyridones. Obtained compounds were characterized via UV-Vis, FT-IR, and H-1 and C-13 NMR spectroscopy as well as by elemental analysis data. The UV-Vis spectra of the synthesized dyes were measured in thirteen solvents of different properties at room temperature. Solvatochromism and tautomerism of novel azo dyes were discussed. An MTT (3,4,5-dimethyl-thiazol-2-yl)-2,5-diphenyl tetrazolium bromide) test was performed to prove the biocompatibility of the investigated dyes. The investigated dyes exhibited satisfying antiproliferative activities against both tumor cell lines, MDA-MB-231 and HCT-116, demonstrating the potent capacity for treatment of tumors.",
publisher = "Scientific Technical Research Council Turkey-Tubitak, Ankara",
journal = "Turkish Journal of Chemistry",
title = "Synthesis, solvatochromism, and biological activity of novel azo dyes bearing 2-pyridone and benzimidazole moieties",
pages = "907-896",
number = "3",
volume = "42",
doi = "10.3906/kim-1711-97"
}

Mijin, D., Božić-Nedeljković, B., Božić, B., Kovrlija, I., Lađarević, J.,& Ušćumlić, G.. (2018). Synthesis, solvatochromism, and biological activity of novel azo dyes bearing 2-pyridone and benzimidazole moieties. in Turkish Journal of Chemistry
Scientific Technical Research Council Turkey-Tubitak, Ankara., 42(3), 896-907.
https://doi.org/10.3906/kim-1711-97

Mijin D, Božić-Nedeljković B, Božić B, Kovrlija I, Lađarević J, Ušćumlić G. Synthesis, solvatochromism, and biological activity of novel azo dyes bearing 2-pyridone and benzimidazole moieties. in Turkish Journal of Chemistry. 2018;42(3):896-907.
doi:10.3906/kim-1711-97 .

Mijin, Dušan, Božić-Nedeljković, Biljana, Božić, Bojan, Kovrlija, Ilijana, Lađarević, Jelena, Ušćumlić, Gordana, "Synthesis, solvatochromism, and biological activity of novel azo dyes bearing 2-pyridone and benzimidazole moieties" in Turkish Journal of Chemistry, 42, no. 3 (2018):896-907,
https://doi.org/10.3906/kim-1711-97 . .

TY  - JOUR
AU  - Filipović, Vuk
AU  - Božić-Nedeljković, Biljana
AU  - Vukomanović, Marija
AU  - Tomić, Simonida
PY  - 2018
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3874
AB  - Gelatin hydrogels have great potential in regenerative medicine but their weak mechanical properties are a major drawback for the load-bearing applications, such as scaffolds for tissue engineering. To overcome this deficiency, novel biodegradable hydrogels with improved mechanical properties were prepared by combining gelatine with 2-hydroxyethyl methacrylate (HEMA), using a double network synthetic procedure. The first, superporous and mechanically strong network, was obtained by free radical polymerization of HEMA at cryogenic temperature, in the presence of gelatin. Degradable poly (beta-amino ester) (PBAE) macromers of different chemical composition or molecular weight were used as crosslinkers to introduce hydrolytically labile bonds in PHEMA. The second gelatin network was formed by crosslinking gelatin with glutaraldehyde. For comparison, a set of biodegradable PHEMA networks was obtained by polymerization of HEMA at cryogenic temperature. All samples were characterized revealing that mechanical strength, swelling behavior and degradation rate as well as high biocompatibility of new IPNs are in accordance with values required for scaffolds in tissue engineering applications and that tuning of these properties is accomplished by simply using different PBAE macromers.
PB  - Elsevier Sci Ltd, Oxford
T2  - Polymer Testing
T1  - Biocompatible and degradable scaffolds based on 2-hydroxyethyl methacrylate, gelatin and poly(beta amino ester) crosslinkers
EP  - 278
SP  - 270
VL  - 68
DO  - 10.1016/j.polymertesting.2018.04.024
ER  - 

@article{
author = "Filipović, Vuk and Božić-Nedeljković, Biljana and Vukomanović, Marija and Tomić, Simonida",
year = "2018",
abstract = "Gelatin hydrogels have great potential in regenerative medicine but their weak mechanical properties are a major drawback for the load-bearing applications, such as scaffolds for tissue engineering. To overcome this deficiency, novel biodegradable hydrogels with improved mechanical properties were prepared by combining gelatine with 2-hydroxyethyl methacrylate (HEMA), using a double network synthetic procedure. The first, superporous and mechanically strong network, was obtained by free radical polymerization of HEMA at cryogenic temperature, in the presence of gelatin. Degradable poly (beta-amino ester) (PBAE) macromers of different chemical composition or molecular weight were used as crosslinkers to introduce hydrolytically labile bonds in PHEMA. The second gelatin network was formed by crosslinking gelatin with glutaraldehyde. For comparison, a set of biodegradable PHEMA networks was obtained by polymerization of HEMA at cryogenic temperature. All samples were characterized revealing that mechanical strength, swelling behavior and degradation rate as well as high biocompatibility of new IPNs are in accordance with values required for scaffolds in tissue engineering applications and that tuning of these properties is accomplished by simply using different PBAE macromers.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Polymer Testing",
title = "Biocompatible and degradable scaffolds based on 2-hydroxyethyl methacrylate, gelatin and poly(beta amino ester) crosslinkers",
pages = "278-270",
volume = "68",
doi = "10.1016/j.polymertesting.2018.04.024"
}

Filipović, V., Božić-Nedeljković, B., Vukomanović, M.,& Tomić, S.. (2018). Biocompatible and degradable scaffolds based on 2-hydroxyethyl methacrylate, gelatin and poly(beta amino ester) crosslinkers. in Polymer Testing
Elsevier Sci Ltd, Oxford., 68, 270-278.
https://doi.org/10.1016/j.polymertesting.2018.04.024

Filipović V, Božić-Nedeljković B, Vukomanović M, Tomić S. Biocompatible and degradable scaffolds based on 2-hydroxyethyl methacrylate, gelatin and poly(beta amino ester) crosslinkers. in Polymer Testing. 2018;68:270-278.
doi:10.1016/j.polymertesting.2018.04.024 .

Filipović, Vuk, Božić-Nedeljković, Biljana, Vukomanović, Marija, Tomić, Simonida, "Biocompatible and degradable scaffolds based on 2-hydroxyethyl methacrylate, gelatin and poly(beta amino ester) crosslinkers" in Polymer Testing, 68 (2018):270-278,
https://doi.org/10.1016/j.polymertesting.2018.04.024 . .

TY  - JOUR
AU  - Božić, Bojan
AU  - Rogan, Jelena
AU  - Poleti, Dejan
AU  - Rančić, Milica
AU  - Trišović, Nemanja
AU  - Božić, Biljana
AU  - Ušćumlić, Gordana
PY  - 2017
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3607
AB  - A series of six novel 2-(5-arylidene-2,4-dioxotetrahydrothiazole-3-yl) propanoic acids and six corresponding methyl esters were synthesized. All compounds were characterized by melting points, elemental analysis, FT-IR, H-1 and C-13 NMR spectroscopy. Crystal structure of methyl-2-(5-(4-methoxybenzylidene)-2,4-dioxotetrahydrothiazole-3-yl) propionate was confirmed by X-ray analysis. The antiproliferative activity of all synthesized compounds against human colon cancer, breast cancer and myelogenous leukemia cell lines, i.e. HCT-116, MDA-231 and K562, respectively, was evaluated. The results indicate that antiproliferative activity of the synthesized esters is better than the activity of the corresponding acids. All synthesized compounds showed significant antiproliferative effects against HCT116 cells in all tested concentrations (0.01-100 lM). Moreover, in vitro antimicrobial activity against a wide range of tested microorganisms was examined.
PB  - Elsevier Science Bv, Amsterdam
T2  - Arabian Journal of Chemistry
T1  - Synthesis, characterization and biological activity of 2-(5-arylidene-2,4-dioxotetrahydrothiazole-3-yl) propanoic acid derivatives
EP  - S2643
SP  - S2637
VL  - 10
DO  - 10.1016/j.arabjc.2013.10.002
ER  - 

@article{
author = "Božić, Bojan and Rogan, Jelena and Poleti, Dejan and Rančić, Milica and Trišović, Nemanja and Božić, Biljana and Ušćumlić, Gordana",
year = "2017",
abstract = "A series of six novel 2-(5-arylidene-2,4-dioxotetrahydrothiazole-3-yl) propanoic acids and six corresponding methyl esters were synthesized. All compounds were characterized by melting points, elemental analysis, FT-IR, H-1 and C-13 NMR spectroscopy. Crystal structure of methyl-2-(5-(4-methoxybenzylidene)-2,4-dioxotetrahydrothiazole-3-yl) propionate was confirmed by X-ray analysis. The antiproliferative activity of all synthesized compounds against human colon cancer, breast cancer and myelogenous leukemia cell lines, i.e. HCT-116, MDA-231 and K562, respectively, was evaluated. The results indicate that antiproliferative activity of the synthesized esters is better than the activity of the corresponding acids. All synthesized compounds showed significant antiproliferative effects against HCT116 cells in all tested concentrations (0.01-100 lM). Moreover, in vitro antimicrobial activity against a wide range of tested microorganisms was examined.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Arabian Journal of Chemistry",
title = "Synthesis, characterization and biological activity of 2-(5-arylidene-2,4-dioxotetrahydrothiazole-3-yl) propanoic acid derivatives",
pages = "S2643-S2637",
volume = "10",
doi = "10.1016/j.arabjc.2013.10.002"
}

Božić, B., Rogan, J., Poleti, D., Rančić, M., Trišović, N., Božić, B.,& Ušćumlić, G.. (2017). Synthesis, characterization and biological activity of 2-(5-arylidene-2,4-dioxotetrahydrothiazole-3-yl) propanoic acid derivatives. in Arabian Journal of Chemistry
Elsevier Science Bv, Amsterdam., 10, S2637-S2643.
https://doi.org/10.1016/j.arabjc.2013.10.002

Božić B, Rogan J, Poleti D, Rančić M, Trišović N, Božić B, Ušćumlić G. Synthesis, characterization and biological activity of 2-(5-arylidene-2,4-dioxotetrahydrothiazole-3-yl) propanoic acid derivatives. in Arabian Journal of Chemistry. 2017;10:S2637-S2643.
doi:10.1016/j.arabjc.2013.10.002 .

Božić, Bojan, Rogan, Jelena, Poleti, Dejan, Rančić, Milica, Trišović, Nemanja, Božić, Biljana, Ušćumlić, Gordana, "Synthesis, characterization and biological activity of 2-(5-arylidene-2,4-dioxotetrahydrothiazole-3-yl) propanoic acid derivatives" in Arabian Journal of Chemistry, 10 (2017):S2637-S2643,
https://doi.org/10.1016/j.arabjc.2013.10.002 . .

TY  - CONF
AU  - Lazić, Anita M.
AU  - Radovanović, Lidija D.
AU  - Božić, Biljana Đ.
AU  - Ušćumlić, Gordana S.
PY  - 2017
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6764
AB  - U okviru proučavanja uticaja strukture na biološku aktivnost derivata cikloalkanspiro-5-hidantoina,
u radu je testirana citotoksičnost novih serija jedinjenja: 3-(4-supstituisanih benzil)-1,3-
-diazaspiro[4.5]dekan-2,4-diona i 3-(4-supstituisanih benzil)-1,3-diazaspiro[4.6] undekan-2,4-
diona. Citotoksična aktivnost određena je MTT testom prema ćelijskoj liniji humanog karcinoma
dojke (MDA-MB-231). Strukturne karakteristike jedinjenja su određene rendgenskom strukturnom
analizom kao i odgovarajućim spektroskopskim metodama. Testirana jedinjenja su pokazala
statistički značajnu antiproliferativnu aktivnost prema ćelijskoj liniji MDA-MB-231 u proučavanom
opsegu koncentracija. Naročito su se istakli derivati koji u okviru svoje strukture sadrže kao
supstituente atome halogena i nitro-grupu. Rezultati su upoređeni sa prethodno određenom
antiproliferativnom aktivnošću za 3-(4-supstituisane benzil)-5,5-difenilhidantoine. Diskutovan je
uticaj strukture na antiproliferativnu aktivnost proučavanih jedinjenja.
AB  - In order to investigate the influence of the structure on the antiproliferative activity of the cycloalkanespiro-5-hydantoine derivatives, the cytotoxity of the new series of compounds: 3-(4- substituted benzyl)-1,3-diazaspiro[4.5]decane-2,4-dione and 3-(4-substituted benzyl)-1,3- -diazaspiro[4.6]undecane-2,4-dione has been tested. Cytotoxic activity was determined by the MTT assay againist the cell line of human breast cancer (MDA-MB-231). The structural characteristics of the compounds are determined by X-ray structural analysis, as well as by appropriate spectroscopic methods. The tested compounds showed statistically significant antiproliferative activity to the MDA-MB-231 cell line in the studied concentration range. Among the most active are derivatives containing as substituents halogen atoms and a nitro group. The results were compared with a predetermined antiproliferative activity for 3-(4-substituted benzil)-5,5- -diphenylhydantoines. The influence of structure on the antiproliferative activity of the studied compounds is also discussed.
PB  - Beograd : Srpsko hemijsko društvo
C3  - Kratki izvodi i Knjiga radova / 54. savetovanje Srpskog hemijskog društva i 5. konferencija mladih hemičara Srbije, Beograd 29. i 30. septembar 2017
T1  - Uticaj strukture na antiproliferativnu aktivnost derivata cikloalkanspiro-5-hidantoina
T1  - The influence of the structure on the antiproliferative activity of the cycloalkanespiro-5-hydantoin derivatives
SP  - 103
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6764
ER  - 

@conference{
author = "Lazić, Anita M. and Radovanović, Lidija D. and Božić, Biljana Đ. and Ušćumlić, Gordana S.",
year = "2017",
abstract = "U okviru proučavanja uticaja strukture na biološku aktivnost derivata cikloalkanspiro-5-hidantoina,
u radu je testirana citotoksičnost novih serija jedinjenja: 3-(4-supstituisanih benzil)-1,3-
-diazaspiro[4.5]dekan-2,4-diona i 3-(4-supstituisanih benzil)-1,3-diazaspiro[4.6] undekan-2,4-
diona. Citotoksična aktivnost određena je MTT testom prema ćelijskoj liniji humanog karcinoma
dojke (MDA-MB-231). Strukturne karakteristike jedinjenja su određene rendgenskom strukturnom
analizom kao i odgovarajućim spektroskopskim metodama. Testirana jedinjenja su pokazala
statistički značajnu antiproliferativnu aktivnost prema ćelijskoj liniji MDA-MB-231 u proučavanom
opsegu koncentracija. Naročito su se istakli derivati koji u okviru svoje strukture sadrže kao
supstituente atome halogena i nitro-grupu. Rezultati su upoređeni sa prethodno određenom
antiproliferativnom aktivnošću za 3-(4-supstituisane benzil)-5,5-difenilhidantoine. Diskutovan je
uticaj strukture na antiproliferativnu aktivnost proučavanih jedinjenja., In order to investigate the influence of the structure on the antiproliferative activity of the cycloalkanespiro-5-hydantoine derivatives, the cytotoxity of the new series of compounds: 3-(4- substituted benzyl)-1,3-diazaspiro[4.5]decane-2,4-dione and 3-(4-substituted benzyl)-1,3- -diazaspiro[4.6]undecane-2,4-dione has been tested. Cytotoxic activity was determined by the MTT assay againist the cell line of human breast cancer (MDA-MB-231). The structural characteristics of the compounds are determined by X-ray structural analysis, as well as by appropriate spectroscopic methods. The tested compounds showed statistically significant antiproliferative activity to the MDA-MB-231 cell line in the studied concentration range. Among the most active are derivatives containing as substituents halogen atoms and a nitro group. The results were compared with a predetermined antiproliferative activity for 3-(4-substituted benzil)-5,5- -diphenylhydantoines. The influence of structure on the antiproliferative activity of the studied compounds is also discussed.",
publisher = "Beograd : Srpsko hemijsko društvo",
journal = "Kratki izvodi i Knjiga radova / 54. savetovanje Srpskog hemijskog društva i 5. konferencija mladih hemičara Srbije, Beograd 29. i 30. septembar 2017",
title = "Uticaj strukture na antiproliferativnu aktivnost derivata cikloalkanspiro-5-hidantoina, The influence of the structure on the antiproliferative activity of the cycloalkanespiro-5-hydantoin derivatives",
pages = "103",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6764"
}

Lazić, A. M., Radovanović, L. D., Božić, B. Đ.,& Ušćumlić, G. S.. (2017). Uticaj strukture na antiproliferativnu aktivnost derivata cikloalkanspiro-5-hidantoina. in Kratki izvodi i Knjiga radova / 54. savetovanje Srpskog hemijskog društva i 5. konferencija mladih hemičara Srbije, Beograd 29. i 30. septembar 2017
Beograd : Srpsko hemijsko društvo., 103.
https://hdl.handle.net/21.15107/rcub_technorep_6764

Lazić AM, Radovanović LD, Božić BĐ, Ušćumlić GS. Uticaj strukture na antiproliferativnu aktivnost derivata cikloalkanspiro-5-hidantoina. in Kratki izvodi i Knjiga radova / 54. savetovanje Srpskog hemijskog društva i 5. konferencija mladih hemičara Srbije, Beograd 29. i 30. septembar 2017. 2017;:103.
https://hdl.handle.net/21.15107/rcub_technorep_6764 .

Lazić, Anita M., Radovanović, Lidija D., Božić, Biljana Đ., Ušćumlić, Gordana S., "Uticaj strukture na antiproliferativnu aktivnost derivata cikloalkanspiro-5-hidantoina" in Kratki izvodi i Knjiga radova / 54. savetovanje Srpskog hemijskog društva i 5. konferencija mladih hemičara Srbije, Beograd 29. i 30. septembar 2017 (2017):103,
https://hdl.handle.net/21.15107/rcub_technorep_6764 .

TY  - CONF
AU  - Lazić, Anita M.
AU  - Božić, Bojan Đ.
AU  - Božić, Biljana Đ.
AU  - Ušćumlić, Gordana S.
PY  - 2016
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6763
AB  - Spirohidantoini pripadaju grupi jedinjenja koja ispoljavaju širok spektar biološke aktivnosti. Do
sada su derivati spirohidantoina identifikovani kao antikonvulzivi, antiaritmici, antidijabetici i
antitumorni agensi. U cilju ispitivanja biološke aktivnosti, testirana je citotoksičnost novih serija
jedinjenja: 3-(4-supstituisanih benzil)-1,3-diazaspiro[4.5]dekan-2,4-diona, 3-(2-(4-supstituisanih
fenil)-2-oksoetil)-1,3-diazaspiro-[4.5]-dekan-2,4-diona, 3-(4-supstituisanih benzil)-1,3-diazaspiro[4.6]undekan-2,4-diona i 3-(2-(4-supstituisanih fenil)-2-oksoetil)-1,3-diazaspiro[4.6]undekan-
-2,4-diona. Citotoksična aktivnost određena je MTT testom prema ćelijskim linijama humane
mijeloidne leukemije (K562), raka debelog creva (HCT-116) i raka dojke (MDA-MB-231). Testirana
jedinjenja pokazala su značajnu citotoksičnost prema svim ćelijskim linijama, a naročito su se
istakli derivati koji u okviru svoje strukture sadrže halogene kao supstituente. Uticaj sternih i
elektronskih svojstava supstituenata na aktivnost proučavanih jedinjenja, analiziran je primenom
QSAR modela.
AB  - Spiroydantoins belong to a group of compounds exhibiting a wide range of biological activities. Until now, the spirohydantoin derivatives are identified as anticonvulsants, antiarrhythmic drugs, antidiabetic agents and antitumor agents. In order to investigate the biological activity was tested on the cytotoxicity of the new series of compounds: 3-(4-substituted benzyl)-1,3-diazaspiro[4.5]decane-2,4-dione, 3-(2-(4-substituted phenyl)-2-oxoethyl)-1,3-diazaspiro[4.5]decane2,4-dione, 3-(4-substituted benzyl)-1,3--diazaspiro-[4.6]undecane-2,4-dione and 3-(2-(4-substituted phenyl)-2-oxoethyl)-1,3-diazaspiro[4.6]-undecane-2,4-dione. Cytotoxic activity was assessed by MTT assay cell lines of human myeloid leukemia (K562), colon (HCT-116) and breast cancer cells (MDA-MB-231). The test compounds showed a significant cytotoxicity to all cell lines, and in particular the derivatives containig within its structure halogens as substituents. The effect of steric and electronic properties of the substituents on activity of studed compounds, was analyzed by QSAR models.
PB  - Beograd : Srpsko hemijsko društvo
C3  - Kratki izvodi radova / Četvrta konferencija mladih hemičara Srbije, Beograd, 5. novembar 2016
T1  - Dizajn, sinteza i antiproliferativna aktivnost novih cikloalkanspiro-5-hidantoinskih derivata: Veza između strukture i aktivnosti
T1  - Design, synthesis and antiproliferative evaluation of novel cycloalkane-spiro-5-hydantoin derivatives: A structure-activity relationship study
SP  - 56
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6763
ER  - 

@conference{
author = "Lazić, Anita M. and Božić, Bojan Đ. and Božić, Biljana Đ. and Ušćumlić, Gordana S.",
year = "2016",
abstract = "Spirohidantoini pripadaju grupi jedinjenja koja ispoljavaju širok spektar biološke aktivnosti. Do
sada su derivati spirohidantoina identifikovani kao antikonvulzivi, antiaritmici, antidijabetici i
antitumorni agensi. U cilju ispitivanja biološke aktivnosti, testirana je citotoksičnost novih serija
jedinjenja: 3-(4-supstituisanih benzil)-1,3-diazaspiro[4.5]dekan-2,4-diona, 3-(2-(4-supstituisanih
fenil)-2-oksoetil)-1,3-diazaspiro-[4.5]-dekan-2,4-diona, 3-(4-supstituisanih benzil)-1,3-diazaspiro[4.6]undekan-2,4-diona i 3-(2-(4-supstituisanih fenil)-2-oksoetil)-1,3-diazaspiro[4.6]undekan-
-2,4-diona. Citotoksična aktivnost određena je MTT testom prema ćelijskim linijama humane
mijeloidne leukemije (K562), raka debelog creva (HCT-116) i raka dojke (MDA-MB-231). Testirana
jedinjenja pokazala su značajnu citotoksičnost prema svim ćelijskim linijama, a naročito su se
istakli derivati koji u okviru svoje strukture sadrže halogene kao supstituente. Uticaj sternih i
elektronskih svojstava supstituenata na aktivnost proučavanih jedinjenja, analiziran je primenom
QSAR modela., Spiroydantoins belong to a group of compounds exhibiting a wide range of biological activities. Until now, the spirohydantoin derivatives are identified as anticonvulsants, antiarrhythmic drugs, antidiabetic agents and antitumor agents. In order to investigate the biological activity was tested on the cytotoxicity of the new series of compounds: 3-(4-substituted benzyl)-1,3-diazaspiro[4.5]decane-2,4-dione, 3-(2-(4-substituted phenyl)-2-oxoethyl)-1,3-diazaspiro[4.5]decane2,4-dione, 3-(4-substituted benzyl)-1,3--diazaspiro-[4.6]undecane-2,4-dione and 3-(2-(4-substituted phenyl)-2-oxoethyl)-1,3-diazaspiro[4.6]-undecane-2,4-dione. Cytotoxic activity was assessed by MTT assay cell lines of human myeloid leukemia (K562), colon (HCT-116) and breast cancer cells (MDA-MB-231). The test compounds showed a significant cytotoxicity to all cell lines, and in particular the derivatives containig within its structure halogens as substituents. The effect of steric and electronic properties of the substituents on activity of studed compounds, was analyzed by QSAR models.",
publisher = "Beograd : Srpsko hemijsko društvo",
journal = "Kratki izvodi radova / Četvrta konferencija mladih hemičara Srbije, Beograd, 5. novembar 2016",
title = "Dizajn, sinteza i antiproliferativna aktivnost novih cikloalkanspiro-5-hidantoinskih derivata: Veza između strukture i aktivnosti, Design, synthesis and antiproliferative evaluation of novel cycloalkane-spiro-5-hydantoin derivatives: A structure-activity relationship study",
pages = "56",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6763"
}

Lazić, A. M., Božić, B. Đ., Božić, B. Đ.,& Ušćumlić, G. S.. (2016). Dizajn, sinteza i antiproliferativna aktivnost novih cikloalkanspiro-5-hidantoinskih derivata: Veza između strukture i aktivnosti. in Kratki izvodi radova / Četvrta konferencija mladih hemičara Srbije, Beograd, 5. novembar 2016
Beograd : Srpsko hemijsko društvo., 56.
https://hdl.handle.net/21.15107/rcub_technorep_6763

Lazić AM, Božić BĐ, Božić BĐ, Ušćumlić GS. Dizajn, sinteza i antiproliferativna aktivnost novih cikloalkanspiro-5-hidantoinskih derivata: Veza između strukture i aktivnosti. in Kratki izvodi radova / Četvrta konferencija mladih hemičara Srbije, Beograd, 5. novembar 2016. 2016;:56.
https://hdl.handle.net/21.15107/rcub_technorep_6763 .

Lazić, Anita M., Božić, Bojan Đ., Božić, Biljana Đ., Ušćumlić, Gordana S., "Dizajn, sinteza i antiproliferativna aktivnost novih cikloalkanspiro-5-hidantoinskih derivata: Veza između strukture i aktivnosti" in Kratki izvodi radova / Četvrta konferencija mladih hemičara Srbije, Beograd, 5. novembar 2016 (2016):56,
https://hdl.handle.net/21.15107/rcub_technorep_6763 .

TY  - JOUR
AU  - Babić, Marija
AU  - Božić, Bojan
AU  - Božić, Biljana
AU  - Filipović, Jovanka
AU  - Ušćumlić, Gordana
AU  - Tomić, Simonida
PY  - 2016
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3409
AB  - A series of dual-sensitive poly(2-hydroxypropyl acrylate/itaconic acid) (P(HPA/IA)) hydrogels were synthesized and evaluated as potential highly effective antiproliferative drug delivery system. Investigated hydrophobic antiproliferative agent, Ni(II) complex with Oxaprozin, was successfully synthesized and efficiently loaded into the'"intelligent" P(HPA/IA) hydrogels. Swelling studies showed that loaded agent did not annul pH- and temperature-sensitivity of the investigated hydrogels. In vitro antiproliferative activity of investigated complex against human cervical (HeLa) and melanoma cancer (FemX) cell lines was tested. The results of in vitro release study at different pH values confirmed synthesized hydrogels loaded with investigated complex as a highly effective pH-triggered drug delivery system for the advanced anticancer therapy as well as for the targeted treatment of intestine/colon cancers.
PB  - Elsevier, Amsterdam
T2  - Materials Letters
T1  - Evaluation of novel antiproliferative controlled drug delivery system based on poly(2-hydroxypropyl acrylate/itaconic acid) hydrogels and nickel complex with Oxaprozin
EP  - 217
SP  - 214
VL  - 163
DO  - 10.1016/j.matlet.2015.10.078
ER  - 

@article{
author = "Babić, Marija and Božić, Bojan and Božić, Biljana and Filipović, Jovanka and Ušćumlić, Gordana and Tomić, Simonida",
year = "2016",
abstract = "A series of dual-sensitive poly(2-hydroxypropyl acrylate/itaconic acid) (P(HPA/IA)) hydrogels were synthesized and evaluated as potential highly effective antiproliferative drug delivery system. Investigated hydrophobic antiproliferative agent, Ni(II) complex with Oxaprozin, was successfully synthesized and efficiently loaded into the'"intelligent" P(HPA/IA) hydrogels. Swelling studies showed that loaded agent did not annul pH- and temperature-sensitivity of the investigated hydrogels. In vitro antiproliferative activity of investigated complex against human cervical (HeLa) and melanoma cancer (FemX) cell lines was tested. The results of in vitro release study at different pH values confirmed synthesized hydrogels loaded with investigated complex as a highly effective pH-triggered drug delivery system for the advanced anticancer therapy as well as for the targeted treatment of intestine/colon cancers.",
publisher = "Elsevier, Amsterdam",
journal = "Materials Letters",
title = "Evaluation of novel antiproliferative controlled drug delivery system based on poly(2-hydroxypropyl acrylate/itaconic acid) hydrogels and nickel complex with Oxaprozin",
pages = "217-214",
volume = "163",
doi = "10.1016/j.matlet.2015.10.078"
}

Babić, M., Božić, B., Božić, B., Filipović, J., Ušćumlić, G.,& Tomić, S.. (2016). Evaluation of novel antiproliferative controlled drug delivery system based on poly(2-hydroxypropyl acrylate/itaconic acid) hydrogels and nickel complex with Oxaprozin. in Materials Letters
Elsevier, Amsterdam., 163, 214-217.
https://doi.org/10.1016/j.matlet.2015.10.078

Babić M, Božić B, Božić B, Filipović J, Ušćumlić G, Tomić S. Evaluation of novel antiproliferative controlled drug delivery system based on poly(2-hydroxypropyl acrylate/itaconic acid) hydrogels and nickel complex with Oxaprozin. in Materials Letters. 2016;163:214-217.
doi:10.1016/j.matlet.2015.10.078 .

Babić, Marija, Božić, Bojan, Božić, Biljana, Filipović, Jovanka, Ušćumlić, Gordana, Tomić, Simonida, "Evaluation of novel antiproliferative controlled drug delivery system based on poly(2-hydroxypropyl acrylate/itaconic acid) hydrogels and nickel complex with Oxaprozin" in Materials Letters, 163 (2016):214-217,
https://doi.org/10.1016/j.matlet.2015.10.078 . .

TY  - JOUR
AU  - Đaković-Sekulić, Tatjana
AU  - Smolinski, Adam
AU  - Trišović, Nemanja
AU  - Ušćumlić, Gordana
AU  - Božić, Biljana
PY  - 2015
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3114
AB  - Cancer is the major health problem affecting the mankind of today. Most of the drugs used in traditional chemotherapy are very limited and the discovery of novel, more active, more selective and less toxic ones is still very intensive. A chemometric approach was applied in the study of antiproliferative activity against human colon cancer and breast cancer as well as in the study of lipophilicity of 3-(4-substitutedbenzyl)-5-ethyl-5-phenyl- and 3-(4-substituted benzyl)-5,5-diphenylhydantoins. Hierarchical clustering analysis (HCA) shows that the investigated hydantoins have higher antiproliferative activity against human breast cancer cells than against human colon cancer cells. However, some hydantoins at the highest applied concentration reverse antiproliferative effect, higher against the human colon cancer cells and lower against human breast cancer cells. Principal component analysis (PCA) gives better insight into the activity of hydantoins related to their structural changes. It distinguishes more active compounds from the less active ones according to various criteria. Generally, more lipophilic 5,5-diphenylhydantoins exhibit a higher antiproliferative activity comparing to less lipophilic 5-ethyl-5-phenylhydantoins. Also, a substituent attached to benzyl moieties affects the activity additionally. The activity is particularly pronounced for compounds with cyano, methyl, chloro and bromo group. Halogen substituent were superior in antiproliferative capacity particularly in the series of 5,5-diphenylhydantoins.
PB  - Soc Brasileira Quimica, Sao Paulo
T2  - Journal of the Brazilian Chemical Society
T1  - Chemometric Study of the Antiproliferative Activity of Some New Hydantoin Derivatives: Assessment of Activity and Chromatographic Lipophilicity Data
EP  - 1386
IS  - 7
SP  - 1379
VL  - 26
DO  - 10.5935/0103-5053.20150106
ER  - 

@article{
author = "Đaković-Sekulić, Tatjana and Smolinski, Adam and Trišović, Nemanja and Ušćumlić, Gordana and Božić, Biljana",
year = "2015",
abstract = "Cancer is the major health problem affecting the mankind of today. Most of the drugs used in traditional chemotherapy are very limited and the discovery of novel, more active, more selective and less toxic ones is still very intensive. A chemometric approach was applied in the study of antiproliferative activity against human colon cancer and breast cancer as well as in the study of lipophilicity of 3-(4-substitutedbenzyl)-5-ethyl-5-phenyl- and 3-(4-substituted benzyl)-5,5-diphenylhydantoins. Hierarchical clustering analysis (HCA) shows that the investigated hydantoins have higher antiproliferative activity against human breast cancer cells than against human colon cancer cells. However, some hydantoins at the highest applied concentration reverse antiproliferative effect, higher against the human colon cancer cells and lower against human breast cancer cells. Principal component analysis (PCA) gives better insight into the activity of hydantoins related to their structural changes. It distinguishes more active compounds from the less active ones according to various criteria. Generally, more lipophilic 5,5-diphenylhydantoins exhibit a higher antiproliferative activity comparing to less lipophilic 5-ethyl-5-phenylhydantoins. Also, a substituent attached to benzyl moieties affects the activity additionally. The activity is particularly pronounced for compounds with cyano, methyl, chloro and bromo group. Halogen substituent were superior in antiproliferative capacity particularly in the series of 5,5-diphenylhydantoins.",
publisher = "Soc Brasileira Quimica, Sao Paulo",
journal = "Journal of the Brazilian Chemical Society",
title = "Chemometric Study of the Antiproliferative Activity of Some New Hydantoin Derivatives: Assessment of Activity and Chromatographic Lipophilicity Data",
pages = "1386-1379",
number = "7",
volume = "26",
doi = "10.5935/0103-5053.20150106"
}

Đaković-Sekulić, T., Smolinski, A., Trišović, N., Ušćumlić, G.,& Božić, B.. (2015). Chemometric Study of the Antiproliferative Activity of Some New Hydantoin Derivatives: Assessment of Activity and Chromatographic Lipophilicity Data. in Journal of the Brazilian Chemical Society
Soc Brasileira Quimica, Sao Paulo., 26(7), 1379-1386.
https://doi.org/10.5935/0103-5053.20150106

Đaković-Sekulić T, Smolinski A, Trišović N, Ušćumlić G, Božić B. Chemometric Study of the Antiproliferative Activity of Some New Hydantoin Derivatives: Assessment of Activity and Chromatographic Lipophilicity Data. in Journal of the Brazilian Chemical Society. 2015;26(7):1379-1386.
doi:10.5935/0103-5053.20150106 .

Đaković-Sekulić, Tatjana, Smolinski, Adam, Trišović, Nemanja, Ušćumlić, Gordana, Božić, Biljana, "Chemometric Study of the Antiproliferative Activity of Some New Hydantoin Derivatives: Assessment of Activity and Chromatographic Lipophilicity Data" in Journal of the Brazilian Chemical Society, 26, no. 7 (2015):1379-1386,
https://doi.org/10.5935/0103-5053.20150106 . .

TY  - JOUR
AU  - Babić, Marija
AU  - Božić, Bojan
AU  - Božić, Biljana
AU  - Filipović, Jovanka M.
AU  - Ušćumlić, Gordana
AU  - Tomić, Simonida
PY  - 2015
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3126
AB  - A series of dual-sensitive poly(2-hydroxyethyl acrylate/itaconic acid) (P(HEA/IA)) hydrogels were synthesized and evaluated as drug delivery systems for potential antiproliferative agents. Investigated hydrophobic compounds, Mn(II) and Zn(II) complexes with Oxaprozin, were efficiently loaded into the P(HEA/IA) hydrogels, which was confirmed by FTIR and UV-Vis spectroscopy. Swelling studies, conducted in the physiological pH range of 2.20-8.00 and in temperature range of 30-50 A degrees C, demonstrated that loaded transition metal complexes in P(HEA/IA) hydrogels did not annul pH and temperature sensitivity of the hydrogels. In vitro antiproliferative activity of Mn(II) and Zn(II) complexes with Oxaprozin against human cervical (HeLa) and melanoma cancer (Fem X) cell lines was tested. Results of in vitro release study investigated at different pH conditions confirmed P(HEA/IA) hydrogels as a highly effective pH-triggered drug delivery system for hydrophobic antiproliferative agents. These performances indicate that P(HEA/IA) hydrogels loaded with transition metal complexes can be further studied as a promising candidate for anticancer therapy, as well as for targeted treatment of intestine/colon cancers.
PB  - Springer, New York
T2  - Journal of Materials Science
T1  - Evaluation of poly(hydroxyethyl acrylate/itaconic acid) hydrogels for controlled delivery of transition metal complexes with Oxaprozin as potential antiproliferative agents
EP  - 6219
IS  - 18
SP  - 6208
VL  - 50
DO  - 10.1007/s10853-015-9179-6
ER  - 

@article{
author = "Babić, Marija and Božić, Bojan and Božić, Biljana and Filipović, Jovanka M. and Ušćumlić, Gordana and Tomić, Simonida",
year = "2015",
abstract = "A series of dual-sensitive poly(2-hydroxyethyl acrylate/itaconic acid) (P(HEA/IA)) hydrogels were synthesized and evaluated as drug delivery systems for potential antiproliferative agents. Investigated hydrophobic compounds, Mn(II) and Zn(II) complexes with Oxaprozin, were efficiently loaded into the P(HEA/IA) hydrogels, which was confirmed by FTIR and UV-Vis spectroscopy. Swelling studies, conducted in the physiological pH range of 2.20-8.00 and in temperature range of 30-50 A degrees C, demonstrated that loaded transition metal complexes in P(HEA/IA) hydrogels did not annul pH and temperature sensitivity of the hydrogels. In vitro antiproliferative activity of Mn(II) and Zn(II) complexes with Oxaprozin against human cervical (HeLa) and melanoma cancer (Fem X) cell lines was tested. Results of in vitro release study investigated at different pH conditions confirmed P(HEA/IA) hydrogels as a highly effective pH-triggered drug delivery system for hydrophobic antiproliferative agents. These performances indicate that P(HEA/IA) hydrogels loaded with transition metal complexes can be further studied as a promising candidate for anticancer therapy, as well as for targeted treatment of intestine/colon cancers.",
publisher = "Springer, New York",
journal = "Journal of Materials Science",
title = "Evaluation of poly(hydroxyethyl acrylate/itaconic acid) hydrogels for controlled delivery of transition metal complexes with Oxaprozin as potential antiproliferative agents",
pages = "6219-6208",
number = "18",
volume = "50",
doi = "10.1007/s10853-015-9179-6"
}

Babić, M., Božić, B., Božić, B., Filipović, J. M., Ušćumlić, G.,& Tomić, S.. (2015). Evaluation of poly(hydroxyethyl acrylate/itaconic acid) hydrogels for controlled delivery of transition metal complexes with Oxaprozin as potential antiproliferative agents. in Journal of Materials Science
Springer, New York., 50(18), 6208-6219.
https://doi.org/10.1007/s10853-015-9179-6

Babić M, Božić B, Božić B, Filipović JM, Ušćumlić G, Tomić S. Evaluation of poly(hydroxyethyl acrylate/itaconic acid) hydrogels for controlled delivery of transition metal complexes with Oxaprozin as potential antiproliferative agents. in Journal of Materials Science. 2015;50(18):6208-6219.
doi:10.1007/s10853-015-9179-6 .

Babić, Marija, Božić, Bojan, Božić, Biljana, Filipović, Jovanka M., Ušćumlić, Gordana, Tomić, Simonida, "Evaluation of poly(hydroxyethyl acrylate/itaconic acid) hydrogels for controlled delivery of transition metal complexes with Oxaprozin as potential antiproliferative agents" in Journal of Materials Science, 50, no. 18 (2015):6208-6219,
https://doi.org/10.1007/s10853-015-9179-6 . .

TY  - JOUR
AU  - Hmuda, Sleem F.
AU  - Trišović, Nemanja
AU  - Rogan, Jelena
AU  - Poleti, Dejan
AU  - Vitnik, Željko
AU  - Vitnik, Vesna
AU  - Valentić, Nataša
AU  - Božić, Biljana
AU  - Ušćumlić, Gordana
PY  - 2014
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2862
AB  - Two new series of hydantoin derivatives, 3-(4-substituted benzyl)-5,5-diphenyl- and 3-(4-substituted benzyl)-5-ethyl-5-phenylhydantoins, were synthesized and their antiproliferative activity was tested against human colon cancer HCT-116 and breast cancer MDA-MB-231 cell lines. The presence of different substituents on both hydantoin and benzyl moieties changed the antiproliferative activity of the investigated hydantoins, whereby most of the compounds showed superior antiproliferative activity against MDA-MB-231 than against the HCT-116 cell line. The structure of three compounds was studied by single-crystal X-ray diffraction. The general structural characteristic is the presence of N-H center dot center dot center dot O hydrogen bonds in crystal packings. The molecular geometry and bonding features of the investigated hydantoins in the ground states were calculated using the density functional method. The relationship between structure and antiproliferative activity was discussed. The data presented in this investigation afford guidelines for the preparation of new hydantoin derivatives with greater antiproliferative activity.
PB  - Springer Wien, Wien
T2  - Monatshefte Fur Chemie
T1  - New derivatives of hydantoin as potential antiproliferative agents: biological and structural characterization in combination with quantum chemical calculations
EP  - 833
IS  - 5
SP  - 821
VL  - 145
DO  - 10.1007/s00706-013-1149-6
ER  - 

@article{
author = "Hmuda, Sleem F. and Trišović, Nemanja and Rogan, Jelena and Poleti, Dejan and Vitnik, Željko and Vitnik, Vesna and Valentić, Nataša and Božić, Biljana and Ušćumlić, Gordana",
year = "2014",
abstract = "Two new series of hydantoin derivatives, 3-(4-substituted benzyl)-5,5-diphenyl- and 3-(4-substituted benzyl)-5-ethyl-5-phenylhydantoins, were synthesized and their antiproliferative activity was tested against human colon cancer HCT-116 and breast cancer MDA-MB-231 cell lines. The presence of different substituents on both hydantoin and benzyl moieties changed the antiproliferative activity of the investigated hydantoins, whereby most of the compounds showed superior antiproliferative activity against MDA-MB-231 than against the HCT-116 cell line. The structure of three compounds was studied by single-crystal X-ray diffraction. The general structural characteristic is the presence of N-H center dot center dot center dot O hydrogen bonds in crystal packings. The molecular geometry and bonding features of the investigated hydantoins in the ground states were calculated using the density functional method. The relationship between structure and antiproliferative activity was discussed. The data presented in this investigation afford guidelines for the preparation of new hydantoin derivatives with greater antiproliferative activity.",
publisher = "Springer Wien, Wien",
journal = "Monatshefte Fur Chemie",
title = "New derivatives of hydantoin as potential antiproliferative agents: biological and structural characterization in combination with quantum chemical calculations",
pages = "833-821",
number = "5",
volume = "145",
doi = "10.1007/s00706-013-1149-6"
}

Hmuda, S. F., Trišović, N., Rogan, J., Poleti, D., Vitnik, Ž., Vitnik, V., Valentić, N., Božić, B.,& Ušćumlić, G.. (2014). New derivatives of hydantoin as potential antiproliferative agents: biological and structural characterization in combination with quantum chemical calculations. in Monatshefte Fur Chemie
Springer Wien, Wien., 145(5), 821-833.
https://doi.org/10.1007/s00706-013-1149-6

Hmuda SF, Trišović N, Rogan J, Poleti D, Vitnik Ž, Vitnik V, Valentić N, Božić B, Ušćumlić G. New derivatives of hydantoin as potential antiproliferative agents: biological and structural characterization in combination with quantum chemical calculations. in Monatshefte Fur Chemie. 2014;145(5):821-833.
doi:10.1007/s00706-013-1149-6 .

Hmuda, Sleem F., Trišović, Nemanja, Rogan, Jelena, Poleti, Dejan, Vitnik, Željko, Vitnik, Vesna, Valentić, Nataša, Božić, Biljana, Ušćumlić, Gordana, "New derivatives of hydantoin as potential antiproliferative agents: biological and structural characterization in combination with quantum chemical calculations" in Monatshefte Fur Chemie, 145, no. 5 (2014):821-833,
https://doi.org/10.1007/s00706-013-1149-6 . .

TY  - JOUR
AU  - Obradović, Ana
AU  - Zizić, Jovana
AU  - Trišović, Nemanja
AU  - Božić, Bojan
AU  - Ušćumlić, Gordana
AU  - Božić, Biljana
AU  - Marković, Snežana
PY  - 2013
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2427
AB  - Antioxidant effects of twelve 3-substituted-5,5-diphenylhydantoins in concentrations of 0.01, 0.1, 1, 10, and 100 mu M on human colon cancer cell line HCT-116 were determined 24 h after treatment. The concentrations of superoxide anion radical (O(2)radical anion), nitrites (NO2-), and total glutathione were determined spectrophotometrically. Results indicated that treatment with all compounds induced a decrease in O(2)radical anion and an increase in NO(2)(-)concentrations in HCT-116 cells. Treatment with all compounds resulted in an increase in glutathione, and this indicates changes in redox homeostasis. Based on the obtained results, the investigated hydantoins act as antioxidants because they decrease the production of superoxide anion radical and increase concentrations of glutathione, but they also induce an increase in nitrite production.
PB  - Tubitak Scientific & Technical Research Council Turkey, Ankara
T2  - Turkish Journal of Biology
T1  - Evaluation of antioxidative effects of twelve 3-substituted-5,5-diphenylhydantoins on human colon cancer cell line HCT-116
EP  - 747
IS  - 6
SP  - 741
VL  - 37
DO  - 10.3906/biy-1302-15
ER  - 

@article{
author = "Obradović, Ana and Zizić, Jovana and Trišović, Nemanja and Božić, Bojan and Ušćumlić, Gordana and Božić, Biljana and Marković, Snežana",
year = "2013",
abstract = "Antioxidant effects of twelve 3-substituted-5,5-diphenylhydantoins in concentrations of 0.01, 0.1, 1, 10, and 100 mu M on human colon cancer cell line HCT-116 were determined 24 h after treatment. The concentrations of superoxide anion radical (O(2)radical anion), nitrites (NO2-), and total glutathione were determined spectrophotometrically. Results indicated that treatment with all compounds induced a decrease in O(2)radical anion and an increase in NO(2)(-)concentrations in HCT-116 cells. Treatment with all compounds resulted in an increase in glutathione, and this indicates changes in redox homeostasis. Based on the obtained results, the investigated hydantoins act as antioxidants because they decrease the production of superoxide anion radical and increase concentrations of glutathione, but they also induce an increase in nitrite production.",
publisher = "Tubitak Scientific & Technical Research Council Turkey, Ankara",
journal = "Turkish Journal of Biology",
title = "Evaluation of antioxidative effects of twelve 3-substituted-5,5-diphenylhydantoins on human colon cancer cell line HCT-116",
pages = "747-741",
number = "6",
volume = "37",
doi = "10.3906/biy-1302-15"
}

Obradović, A., Zizić, J., Trišović, N., Božić, B., Ušćumlić, G., Božić, B.,& Marković, S.. (2013). Evaluation of antioxidative effects of twelve 3-substituted-5,5-diphenylhydantoins on human colon cancer cell line HCT-116. in Turkish Journal of Biology
Tubitak Scientific & Technical Research Council Turkey, Ankara., 37(6), 741-747.
https://doi.org/10.3906/biy-1302-15

Obradović A, Zizić J, Trišović N, Božić B, Ušćumlić G, Božić B, Marković S. Evaluation of antioxidative effects of twelve 3-substituted-5,5-diphenylhydantoins on human colon cancer cell line HCT-116. in Turkish Journal of Biology. 2013;37(6):741-747.
doi:10.3906/biy-1302-15 .

Obradović, Ana, Zizić, Jovana, Trišović, Nemanja, Božić, Bojan, Ušćumlić, Gordana, Božić, Biljana, Marković, Snežana, "Evaluation of antioxidative effects of twelve 3-substituted-5,5-diphenylhydantoins on human colon cancer cell line HCT-116" in Turkish Journal of Biology, 37, no. 6 (2013):741-747,
https://doi.org/10.3906/biy-1302-15 . .

TY  - JOUR
AU  - Božić, Bojan
AU  - Rogan, Jelena
AU  - Poleti, Dejan
AU  - Trišović, Nemanja
AU  - Božić, Biljana
AU  - Ušćumlić, Gordana
PY  - 2012
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1970
AB  - A series of novel Mn(II), Co(II), Ni(II), Cu(II) and Zn(II) complexes with oxaprozin (Hoxa), a nonsteroidal anti-inflammatory drug, has been synthesized. The drug and complexes have been characterized by elemental and thermogravimetric (TG) analysis, Fourier transform (FT)-IR, H-1-NMR, C-13-NMR, UV-Vis spectroscopy and magnetic susceptibility measurements. The (pseudo)octahedral geometry has been proposed for all complexes based on electronic spectra and magnetic moments. With exception of the Cu(II) complex, where bridging bidentate mode of COO groups has been found, FT-IR spectra confirmed chelately coordinated COO groups in the other complexes. The general formula of the complexes is [M(H2O)(2)(oxa)(2)]center dot xH(2)O, with x=2 for M=Mn, Co and Ni and x=1.5 for Zn. The binuclear Cu(II) complex, [Cu-2(H2O)(2)(OH)(oxa)(3)]center dot 2H(2)O, has strong Cu-Cu interactions of antiferromagnetic type. The complexes and Hoxa did not exhibit the cytotoxic effect to peritoneal macrophages. For the first time these complexes have been tested for their in vitro antiproliferative activity against human colon and breast cancer cell lines, HCT-116 and MDA-231, respectively. For all investigated compounds significant antiproliferative effects have been observed. Ni(II) complex has been shown to be a promising antiproliferative agent exerting excellent activity against HCT-116 even in nanomolar concentrations.
T2  - Chemical and Pharmaceutical Bulletin
T1  - Synthesis, Characterization and Antiproliferative Activity of Transition Metal Complexes with 3-(4,5-Diphenyl-1,3-oxazol-2-yl)propanoic Acid (Oxaprozin)
EP  - 869
IS  - 7
SP  - 865
VL  - 60
DO  - 10.1248/cpb.c12-00185
ER  - 

@article{
author = "Božić, Bojan and Rogan, Jelena and Poleti, Dejan and Trišović, Nemanja and Božić, Biljana and Ušćumlić, Gordana",
year = "2012",
abstract = "A series of novel Mn(II), Co(II), Ni(II), Cu(II) and Zn(II) complexes with oxaprozin (Hoxa), a nonsteroidal anti-inflammatory drug, has been synthesized. The drug and complexes have been characterized by elemental and thermogravimetric (TG) analysis, Fourier transform (FT)-IR, H-1-NMR, C-13-NMR, UV-Vis spectroscopy and magnetic susceptibility measurements. The (pseudo)octahedral geometry has been proposed for all complexes based on electronic spectra and magnetic moments. With exception of the Cu(II) complex, where bridging bidentate mode of COO groups has been found, FT-IR spectra confirmed chelately coordinated COO groups in the other complexes. The general formula of the complexes is [M(H2O)(2)(oxa)(2)]center dot xH(2)O, with x=2 for M=Mn, Co and Ni and x=1.5 for Zn. The binuclear Cu(II) complex, [Cu-2(H2O)(2)(OH)(oxa)(3)]center dot 2H(2)O, has strong Cu-Cu interactions of antiferromagnetic type. The complexes and Hoxa did not exhibit the cytotoxic effect to peritoneal macrophages. For the first time these complexes have been tested for their in vitro antiproliferative activity against human colon and breast cancer cell lines, HCT-116 and MDA-231, respectively. For all investigated compounds significant antiproliferative effects have been observed. Ni(II) complex has been shown to be a promising antiproliferative agent exerting excellent activity against HCT-116 even in nanomolar concentrations.",
journal = "Chemical and Pharmaceutical Bulletin",
title = "Synthesis, Characterization and Antiproliferative Activity of Transition Metal Complexes with 3-(4,5-Diphenyl-1,3-oxazol-2-yl)propanoic Acid (Oxaprozin)",
pages = "869-865",
number = "7",
volume = "60",
doi = "10.1248/cpb.c12-00185"
}

Božić, B., Rogan, J., Poleti, D., Trišović, N., Božić, B.,& Ušćumlić, G.. (2012). Synthesis, Characterization and Antiproliferative Activity of Transition Metal Complexes with 3-(4,5-Diphenyl-1,3-oxazol-2-yl)propanoic Acid (Oxaprozin). in Chemical and Pharmaceutical Bulletin, 60(7), 865-869.
https://doi.org/10.1248/cpb.c12-00185

Božić B, Rogan J, Poleti D, Trišović N, Božić B, Ušćumlić G. Synthesis, Characterization and Antiproliferative Activity of Transition Metal Complexes with 3-(4,5-Diphenyl-1,3-oxazol-2-yl)propanoic Acid (Oxaprozin). in Chemical and Pharmaceutical Bulletin. 2012;60(7):865-869.
doi:10.1248/cpb.c12-00185 .

Božić, Bojan, Rogan, Jelena, Poleti, Dejan, Trišović, Nemanja, Božić, Biljana, Ušćumlić, Gordana, "Synthesis, Characterization and Antiproliferative Activity of Transition Metal Complexes with 3-(4,5-Diphenyl-1,3-oxazol-2-yl)propanoic Acid (Oxaprozin)" in Chemical and Pharmaceutical Bulletin, 60, no. 7 (2012):865-869,
https://doi.org/10.1248/cpb.c12-00185 . .

TY  - JOUR
AU  - Trišović, Nemanja
AU  - Božić, Bojan
AU  - Obradović, Ana
AU  - Stefanović, Olgica
AU  - Marković, Snežana
AU  - Čomić, Ljiljana
AU  - Božić, Biljana
AU  - Ušćumlić, Gordana
PY  - 2011
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1797
AB  - A series of twelve 3-substituted-5,5-diphenylhydantoins was synthesized, including some whose anticonvulsant activities have already been reported in the literature. Their antiproliferative activities against HCT-116 human colon carcinoma cells were evaluated to determine structure-activity relationships. Almost all of the compounds exhibited statistically significant antiproliferative effects at a concentration of 100 μM, while the derivative bearing a benzyl group was active even at lower concentrations. Moreover, their in vitro antibacterial activities against Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 25923 and clinical isolates of Escherichia coli, Proteus mirabilis, Pseudomonas aeruginosa, Enterococcus faecalis and Staphylococcus aureus were evaluated. Only the 3-isopropyl and 3-benzyl derivatives showed weak antibacterial activities against the Gram-positive bacterium E. faecalis and the Gram-negative bacteria E. coli ATCC 25922 and E. coli.
AB  - Sintetisana je serija od dvanaest 3-supstituisanih-5,5-difenilhidantoina, koja obuhvata neke od derivata čije su antikonvulzivne aktivnosti poznate u literaturi. Određena je njihova antiproliferativna aktivnost prema ćelijskoj liniji humanog karcinoma kolona, kako bi se utvrdio uticaj strukture na aktivnost. Skoro sva jedinjenja ispoljavaju antiproliferativan efekat u koncentraciji od 100 μM, dok je derivat sa benzil grupom aktivan i u nižim koncentracijama. Dodatno je određena i antibakterijska aktivnost proučavanih jedinjenja prema Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 25923 i kliničkim izolatima Escherichia coli, Proteus mirabilis, Pseudomonas aeruginosa, Enterococcus faecalis i Staphylococcus aureus. 3-Izopropil i 3-benzil derivati pokazuju slabu aktivnost prema gram-pozitivnoj bakteriji E. faecalis i gram-negativnim bakterijama E. coli ATCC 25922 i E. coli.
PB  - Serbian Chemical Society, Belgrade
T2  - Journal of the Serbian Chemical Society
T1  - Structure-activity relationships of 3-substituted-5,5-diphenylhydantoins as potential antiproliferative and antimicrobial agents
T1  - Uticaj strukture na antiproliferativnu i antibakterijsku aktivnost 3-supstituisanih-5,5-difenilhidantoina
EP  - 1606
IS  - 12
SP  - 1597
VL  - 76
UR  - https://hdl.handle.net/21.15107/rcub_technorep_1797
ER  - 

@article{
author = "Trišović, Nemanja and Božić, Bojan and Obradović, Ana and Stefanović, Olgica and Marković, Snežana and Čomić, Ljiljana and Božić, Biljana and Ušćumlić, Gordana",
year = "2011",
abstract = "A series of twelve 3-substituted-5,5-diphenylhydantoins was synthesized, including some whose anticonvulsant activities have already been reported in the literature. Their antiproliferative activities against HCT-116 human colon carcinoma cells were evaluated to determine structure-activity relationships. Almost all of the compounds exhibited statistically significant antiproliferative effects at a concentration of 100 μM, while the derivative bearing a benzyl group was active even at lower concentrations. Moreover, their in vitro antibacterial activities against Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 25923 and clinical isolates of Escherichia coli, Proteus mirabilis, Pseudomonas aeruginosa, Enterococcus faecalis and Staphylococcus aureus were evaluated. Only the 3-isopropyl and 3-benzyl derivatives showed weak antibacterial activities against the Gram-positive bacterium E. faecalis and the Gram-negative bacteria E. coli ATCC 25922 and E. coli., Sintetisana je serija od dvanaest 3-supstituisanih-5,5-difenilhidantoina, koja obuhvata neke od derivata čije su antikonvulzivne aktivnosti poznate u literaturi. Određena je njihova antiproliferativna aktivnost prema ćelijskoj liniji humanog karcinoma kolona, kako bi se utvrdio uticaj strukture na aktivnost. Skoro sva jedinjenja ispoljavaju antiproliferativan efekat u koncentraciji od 100 μM, dok je derivat sa benzil grupom aktivan i u nižim koncentracijama. Dodatno je određena i antibakterijska aktivnost proučavanih jedinjenja prema Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 25923 i kliničkim izolatima Escherichia coli, Proteus mirabilis, Pseudomonas aeruginosa, Enterococcus faecalis i Staphylococcus aureus. 3-Izopropil i 3-benzil derivati pokazuju slabu aktivnost prema gram-pozitivnoj bakteriji E. faecalis i gram-negativnim bakterijama E. coli ATCC 25922 i E. coli.",
publisher = "Serbian Chemical Society, Belgrade",
journal = "Journal of the Serbian Chemical Society",
title = "Structure-activity relationships of 3-substituted-5,5-diphenylhydantoins as potential antiproliferative and antimicrobial agents, Uticaj strukture na antiproliferativnu i antibakterijsku aktivnost 3-supstituisanih-5,5-difenilhidantoina",
pages = "1606-1597",
number = "12",
volume = "76",
url = "https://hdl.handle.net/21.15107/rcub_technorep_1797"
}

Trišović, N., Božić, B., Obradović, A., Stefanović, O., Marković, S., Čomić, L., Božić, B.,& Ušćumlić, G.. (2011). Structure-activity relationships of 3-substituted-5,5-diphenylhydantoins as potential antiproliferative and antimicrobial agents. in Journal of the Serbian Chemical Society
Serbian Chemical Society, Belgrade., 76(12), 1597-1606.
https://hdl.handle.net/21.15107/rcub_technorep_1797

Trišović N, Božić B, Obradović A, Stefanović O, Marković S, Čomić L, Božić B, Ušćumlić G. Structure-activity relationships of 3-substituted-5,5-diphenylhydantoins as potential antiproliferative and antimicrobial agents. in Journal of the Serbian Chemical Society. 2011;76(12):1597-1606.
https://hdl.handle.net/21.15107/rcub_technorep_1797 .

Trišović, Nemanja, Božić, Bojan, Obradović, Ana, Stefanović, Olgica, Marković, Snežana, Čomić, Ljiljana, Božić, Biljana, Ušćumlić, Gordana, "Structure-activity relationships of 3-substituted-5,5-diphenylhydantoins as potential antiproliferative and antimicrobial agents" in Journal of the Serbian Chemical Society, 76, no. 12 (2011):1597-1606,
https://hdl.handle.net/21.15107/rcub_technorep_1797 .