TY  - JOUR
AU  - Ilić, Nevena
AU  - Davidović, Slađana
AU  - Milić, Marija
AU  - Rajilić-Stojanović, Mirjana
AU  - Pecarski, Danijela
AU  - Ivancic-Santek, Mirela
AU  - Mihajlovski, Katarina
AU  - Dimitrijević-Branković, Suzana
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5001
AB  - This study aimed at the exploitation of lignocellulosic wastes for the evaluation of the newly isolated white-rot fungal strains enzymatic potential for bioethanol production. The isolates belonging to Basidiomycetes, Fomes fomentarius TMF2, Schizophyllum commune TMF3, and Bjerkandera adusta TMF1, could synthesize extracellular laccase and various hydrolase while growing on lignocellulosic waste materials. More specifically, for the first time, F. fomentarius TMF2 synthesized laccase using sunflower meal as a substrate. This substrate could stimulate B. adusta TMF1 for carboxymethyl cellulase and Avicelase production. The isolate B. adusta TMF1 was able to produce amylase during its growth on brewer's spent grain, which is up to now the best result reported for this activity of any B. adusta strain. Soybean meal was the most potent substrate for stimulating pectinase production by B. adusta TMF1 and S. commune TMF3. While growing on brewer 's spent grain, B. adusta TMF1 and S. commune TMF3 produced high levels of xylanase. Spent coffee residues were for the first time tested as a substrate for hydrolase production by selected fungal species. Also, this is the first attempt where the produced enzymes by isolate B. adusta TMF1 were used for lignocellulose hydrolysis of brewer's spent grain and corn stover for bioethanol production, where under non-optimized conditions 0.94 g/L and 0.86 g/L of bioethanol could be produced, respectively. This study showed that novel white-rot fungal isolates, especially B. adusta TMF1, could grow on unexploited, low-cost lignocellulosic substrates and to produce biotechnological value-added products within environmental and economical accepted processes.
T2  - Biomass Conversion and Biorefinery
T1  - Valorization of lignocellulosic wastes for extracellular enzyme production by novel Basidiomycetes: screening, hydrolysis, and bioethanol production
EP  - 17186
SP  - 17175
VL  - 13
DO  - 10.1007/s13399-021-02145-x
ER  - 

@article{
author = "Ilić, Nevena and Davidović, Slađana and Milić, Marija and Rajilić-Stojanović, Mirjana and Pecarski, Danijela and Ivancic-Santek, Mirela and Mihajlovski, Katarina and Dimitrijević-Branković, Suzana",
year = "2022",
abstract = "This study aimed at the exploitation of lignocellulosic wastes for the evaluation of the newly isolated white-rot fungal strains enzymatic potential for bioethanol production. The isolates belonging to Basidiomycetes, Fomes fomentarius TMF2, Schizophyllum commune TMF3, and Bjerkandera adusta TMF1, could synthesize extracellular laccase and various hydrolase while growing on lignocellulosic waste materials. More specifically, for the first time, F. fomentarius TMF2 synthesized laccase using sunflower meal as a substrate. This substrate could stimulate B. adusta TMF1 for carboxymethyl cellulase and Avicelase production. The isolate B. adusta TMF1 was able to produce amylase during its growth on brewer's spent grain, which is up to now the best result reported for this activity of any B. adusta strain. Soybean meal was the most potent substrate for stimulating pectinase production by B. adusta TMF1 and S. commune TMF3. While growing on brewer 's spent grain, B. adusta TMF1 and S. commune TMF3 produced high levels of xylanase. Spent coffee residues were for the first time tested as a substrate for hydrolase production by selected fungal species. Also, this is the first attempt where the produced enzymes by isolate B. adusta TMF1 were used for lignocellulose hydrolysis of brewer's spent grain and corn stover for bioethanol production, where under non-optimized conditions 0.94 g/L and 0.86 g/L of bioethanol could be produced, respectively. This study showed that novel white-rot fungal isolates, especially B. adusta TMF1, could grow on unexploited, low-cost lignocellulosic substrates and to produce biotechnological value-added products within environmental and economical accepted processes.",
journal = "Biomass Conversion and Biorefinery",
title = "Valorization of lignocellulosic wastes for extracellular enzyme production by novel Basidiomycetes: screening, hydrolysis, and bioethanol production",
pages = "17186-17175",
volume = "13",
doi = "10.1007/s13399-021-02145-x"
}

Ilić, N., Davidović, S., Milić, M., Rajilić-Stojanović, M., Pecarski, D., Ivancic-Santek, M., Mihajlovski, K.,& Dimitrijević-Branković, S.. (2022). Valorization of lignocellulosic wastes for extracellular enzyme production by novel Basidiomycetes: screening, hydrolysis, and bioethanol production. in Biomass Conversion and Biorefinery, 13, 17175-17186.
https://doi.org/10.1007/s13399-021-02145-x

Ilić N, Davidović S, Milić M, Rajilić-Stojanović M, Pecarski D, Ivancic-Santek M, Mihajlovski K, Dimitrijević-Branković S. Valorization of lignocellulosic wastes for extracellular enzyme production by novel Basidiomycetes: screening, hydrolysis, and bioethanol production. in Biomass Conversion and Biorefinery. 2022;13:17175-17186.
doi:10.1007/s13399-021-02145-x .

Ilić, Nevena, Davidović, Slađana, Milić, Marija, Rajilić-Stojanović, Mirjana, Pecarski, Danijela, Ivancic-Santek, Mirela, Mihajlovski, Katarina, Dimitrijević-Branković, Suzana, "Valorization of lignocellulosic wastes for extracellular enzyme production by novel Basidiomycetes: screening, hydrolysis, and bioethanol production" in Biomass Conversion and Biorefinery, 13 (2022):17175-17186,
https://doi.org/10.1007/s13399-021-02145-x . .

TY  - JOUR
AU  - Pavlović, Neda
AU  - Mijalković, Jelena
AU  - Đorđević, Verica
AU  - Pecarski, Danijela
AU  - Bugarski, Branko
AU  - Knežević-Jugović, Zorica
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5384
AB  - This study presents the state-of-art research about the assembly of soy proteins in nanocarriers, liposomes, and its design includes different physicochemical strategies and approaches: two-step enzymatic hydrolysis of soy concentrate, hydrolysate encapsulation by using phospholipids and cholesterol, and application of ultrasonication. Achieved results revealed that ultrasonication, together with cholesterol addition into phospholipid layers, improved the stability of nanoliposomes, and a maximum EE value of 60.5 % was obtained. Average size of peptide-loaded nanoliposomes was found to be from 191.1 to 286.7 nm, with a ζ potential of −25.5 to −34.6 mV, and a polydispersity index of 0.250–0.390. Ultrasound-assisted encapsulation process did not lead to a decrease in the antioxidant activity of the trapped peptides. FTIR has indicated an effective hydrophobic interaction between phosphatidylcholine and hydrolysate peptides. TEM and SEM have confirmed the spherical nanocarrier structure and unilamelarity. Prolonged gastrointestinal release and stability of peptides have been enabled by liposome nanocarriers.
PB  - Elsevier Ltd.
T2  - Food Chemistry: X
T1  - Ultrasonication for production of nanoliposomes with encapsulated soy protein concentrate hydrolysate: Process optimization, vesicle characteristics and in vitro digestion
SP  - 100370
VL  - 15
DO  - 10.1016/j.fochx.2022.100370
ER  - 

@article{
author = "Pavlović, Neda and Mijalković, Jelena and Đorđević, Verica and Pecarski, Danijela and Bugarski, Branko and Knežević-Jugović, Zorica",
year = "2022",
abstract = "This study presents the state-of-art research about the assembly of soy proteins in nanocarriers, liposomes, and its design includes different physicochemical strategies and approaches: two-step enzymatic hydrolysis of soy concentrate, hydrolysate encapsulation by using phospholipids and cholesterol, and application of ultrasonication. Achieved results revealed that ultrasonication, together with cholesterol addition into phospholipid layers, improved the stability of nanoliposomes, and a maximum EE value of 60.5 % was obtained. Average size of peptide-loaded nanoliposomes was found to be from 191.1 to 286.7 nm, with a ζ potential of −25.5 to −34.6 mV, and a polydispersity index of 0.250–0.390. Ultrasound-assisted encapsulation process did not lead to a decrease in the antioxidant activity of the trapped peptides. FTIR has indicated an effective hydrophobic interaction between phosphatidylcholine and hydrolysate peptides. TEM and SEM have confirmed the spherical nanocarrier structure and unilamelarity. Prolonged gastrointestinal release and stability of peptides have been enabled by liposome nanocarriers.",
publisher = "Elsevier Ltd.",
journal = "Food Chemistry: X",
title = "Ultrasonication for production of nanoliposomes with encapsulated soy protein concentrate hydrolysate: Process optimization, vesicle characteristics and in vitro digestion",
pages = "100370",
volume = "15",
doi = "10.1016/j.fochx.2022.100370"
}

Pavlović, N., Mijalković, J., Đorđević, V., Pecarski, D., Bugarski, B.,& Knežević-Jugović, Z.. (2022). Ultrasonication for production of nanoliposomes with encapsulated soy protein concentrate hydrolysate: Process optimization, vesicle characteristics and in vitro digestion. in Food Chemistry: X
Elsevier Ltd.., 15, 100370.
https://doi.org/10.1016/j.fochx.2022.100370

Pavlović N, Mijalković J, Đorđević V, Pecarski D, Bugarski B, Knežević-Jugović Z. Ultrasonication for production of nanoliposomes with encapsulated soy protein concentrate hydrolysate: Process optimization, vesicle characteristics and in vitro digestion. in Food Chemistry: X. 2022;15:100370.
doi:10.1016/j.fochx.2022.100370 .

Pavlović, Neda, Mijalković, Jelena, Đorđević, Verica, Pecarski, Danijela, Bugarski, Branko, Knežević-Jugović, Zorica, "Ultrasonication for production of nanoliposomes with encapsulated soy protein concentrate hydrolysate: Process optimization, vesicle characteristics and in vitro digestion" in Food Chemistry: X, 15 (2022):100370,
https://doi.org/10.1016/j.fochx.2022.100370 . .

TY  - JOUR
AU  - Mihajlovski, Katarina
AU  - Milić, Marija
AU  - Pecarski, Danijela
AU  - Dimitrijević-Branković, Suzana
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4853
AB  - A recent trend in sustainable bioethanol production is the use of agricultural waste or food waste as an inexpensive and the most available feedstock. Bread waste is the major food waste that could be successfully used for the production of bioethanol. The aim of this study was to optimize ethanol production by the response surface methodology (RSM) using waste bread hydrolysate. Waste bread hydrolysate was obtained using crude hydrolytic enzymes that produce bacterial isolate Hymenobacter sp. CKS3. The influence of time of fermentation (24-72 h) and waste brewer's yeast inoculum (1-4 %) on ethanol production was studied. The optimal conditions, obtained by central composite design (CCD), were 48.6 h of fermentation and 2.85 % of inoculum. Under these conditions, a maximum of 2.06 % of ethanol concentration was reached. The obtained ethanol concentration was in good correlation, coefficient of 0.858, with yeast cell yield. The results obtained in this study imply that waste bread hydrolysate could be used as a biomass source for biofuel production with multiple benefits relating to environmental protection, reduction of production costs, and saving fossil fuels.
T2  - Journal of the Serbian Chemical Society
T1  - Statistical optimization of bioethanol production from waste bread hydrolysate
EP  - 662
IS  - 7-8
SP  - 651
VL  - 86
DO  - 10.2298/JSC210308032M
ER  - 

@article{
author = "Mihajlovski, Katarina and Milić, Marija and Pecarski, Danijela and Dimitrijević-Branković, Suzana",
year = "2021",
abstract = "A recent trend in sustainable bioethanol production is the use of agricultural waste or food waste as an inexpensive and the most available feedstock. Bread waste is the major food waste that could be successfully used for the production of bioethanol. The aim of this study was to optimize ethanol production by the response surface methodology (RSM) using waste bread hydrolysate. Waste bread hydrolysate was obtained using crude hydrolytic enzymes that produce bacterial isolate Hymenobacter sp. CKS3. The influence of time of fermentation (24-72 h) and waste brewer's yeast inoculum (1-4 %) on ethanol production was studied. The optimal conditions, obtained by central composite design (CCD), were 48.6 h of fermentation and 2.85 % of inoculum. Under these conditions, a maximum of 2.06 % of ethanol concentration was reached. The obtained ethanol concentration was in good correlation, coefficient of 0.858, with yeast cell yield. The results obtained in this study imply that waste bread hydrolysate could be used as a biomass source for biofuel production with multiple benefits relating to environmental protection, reduction of production costs, and saving fossil fuels.",
journal = "Journal of the Serbian Chemical Society",
title = "Statistical optimization of bioethanol production from waste bread hydrolysate",
pages = "662-651",
number = "7-8",
volume = "86",
doi = "10.2298/JSC210308032M"
}

Mihajlovski, K., Milić, M., Pecarski, D.,& Dimitrijević-Branković, S.. (2021). Statistical optimization of bioethanol production from waste bread hydrolysate. in Journal of the Serbian Chemical Society, 86(7-8), 651-662.
https://doi.org/10.2298/JSC210308032M

Mihajlovski K, Milić M, Pecarski D, Dimitrijević-Branković S. Statistical optimization of bioethanol production from waste bread hydrolysate. in Journal of the Serbian Chemical Society. 2021;86(7-8):651-662.
doi:10.2298/JSC210308032M .

Mihajlovski, Katarina, Milić, Marija, Pecarski, Danijela, Dimitrijević-Branković, Suzana, "Statistical optimization of bioethanol production from waste bread hydrolysate" in Journal of the Serbian Chemical Society, 86, no. 7-8 (2021):651-662,
https://doi.org/10.2298/JSC210308032M . .

TY  - JOUR
AU  - Mihajlovski, Katarina
AU  - Pecarski, Danijela
AU  - Rajilić-Stojanović, Mirjana
AU  - Dimitrijević-Branković, Suzana
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4827
AB  - During the last few decades, energy demand is increasing rapidly. Concerning this, the use of renewables - lignocellulose biomass, for bioethanol production, as an efficient alternative to replacing fossil fuels, is highly recommended. In this study, valorization of two agricultural wastes was used for various hydrolytic enzyme production by Hymenobacter sp. CKS3, lignocellulosic hydrolysis, and bioethanol production. Conditions for obtaining maximum enzyme production, using agro-industrial waste - molasses and corn stover, were statistically optimized. Under the optimal conditions, in a medium containing 5.0% corn stover, 2.5% molasses, and during 94.55 h (similar to 4 days) of fermentation, the maximum enzymatic activity was achieved - CMCase 1.11 IU/ml, Avicelase 0.92 IU/ml, and pectinase 3.69 IU/ml. The obtained crude enzyme mixture was further used for enzymatic hydrolysis of non-treated corn stover and bioethanol production. The reducing sugar yield of 3.85 g/l was obtained under optimal conditions (corn stover 6.6% and time of hydrolysis 78.8 h (similar to 3 days and 7 h)). Scanning electron microscopy revealed structural changes in corn stover samples after enzymatic hydrolysis. Under non-optimized conditions, 0.37% of ethanol was produced by waste brewer's yeast. The obtained results show that bacteria belonging to the genus Hymenobacter have a still unexplored enzymatic potential that could be used for sustainable production of biotechnologically value-added products including biofuels. Specifically, for the first time, a soil bacterium, classified within the genus Hymenobacter, was used for cellulases (CMCase and Avicelase) and pectinase production.
T2  - Environmental Technology & Innovation
T1  - Valorization of corn stover and molasses for enzyme synthesis, lignocellulosic hydrolysis and bioethanol production by Hymenobacter sp. CKS3
VL  - 23
DO  - 10.1016/j.eti.2021.101627
ER  - 

@article{
author = "Mihajlovski, Katarina and Pecarski, Danijela and Rajilić-Stojanović, Mirjana and Dimitrijević-Branković, Suzana",
year = "2021",
abstract = "During the last few decades, energy demand is increasing rapidly. Concerning this, the use of renewables - lignocellulose biomass, for bioethanol production, as an efficient alternative to replacing fossil fuels, is highly recommended. In this study, valorization of two agricultural wastes was used for various hydrolytic enzyme production by Hymenobacter sp. CKS3, lignocellulosic hydrolysis, and bioethanol production. Conditions for obtaining maximum enzyme production, using agro-industrial waste - molasses and corn stover, were statistically optimized. Under the optimal conditions, in a medium containing 5.0% corn stover, 2.5% molasses, and during 94.55 h (similar to 4 days) of fermentation, the maximum enzymatic activity was achieved - CMCase 1.11 IU/ml, Avicelase 0.92 IU/ml, and pectinase 3.69 IU/ml. The obtained crude enzyme mixture was further used for enzymatic hydrolysis of non-treated corn stover and bioethanol production. The reducing sugar yield of 3.85 g/l was obtained under optimal conditions (corn stover 6.6% and time of hydrolysis 78.8 h (similar to 3 days and 7 h)). Scanning electron microscopy revealed structural changes in corn stover samples after enzymatic hydrolysis. Under non-optimized conditions, 0.37% of ethanol was produced by waste brewer's yeast. The obtained results show that bacteria belonging to the genus Hymenobacter have a still unexplored enzymatic potential that could be used for sustainable production of biotechnologically value-added products including biofuels. Specifically, for the first time, a soil bacterium, classified within the genus Hymenobacter, was used for cellulases (CMCase and Avicelase) and pectinase production.",
journal = "Environmental Technology & Innovation",
title = "Valorization of corn stover and molasses for enzyme synthesis, lignocellulosic hydrolysis and bioethanol production by Hymenobacter sp. CKS3",
volume = "23",
doi = "10.1016/j.eti.2021.101627"
}

Mihajlovski, K., Pecarski, D., Rajilić-Stojanović, M.,& Dimitrijević-Branković, S.. (2021). Valorization of corn stover and molasses for enzyme synthesis, lignocellulosic hydrolysis and bioethanol production by Hymenobacter sp. CKS3. in Environmental Technology & Innovation, 23.
https://doi.org/10.1016/j.eti.2021.101627

Mihajlovski K, Pecarski D, Rajilić-Stojanović M, Dimitrijević-Branković S. Valorization of corn stover and molasses for enzyme synthesis, lignocellulosic hydrolysis and bioethanol production by Hymenobacter sp. CKS3. in Environmental Technology & Innovation. 2021;23.
doi:10.1016/j.eti.2021.101627 .

Mihajlovski, Katarina, Pecarski, Danijela, Rajilić-Stojanović, Mirjana, Dimitrijević-Branković, Suzana, "Valorization of corn stover and molasses for enzyme synthesis, lignocellulosic hydrolysis and bioethanol production by Hymenobacter sp. CKS3" in Environmental Technology & Innovation, 23 (2021),
https://doi.org/10.1016/j.eti.2021.101627 . .

TY  - JOUR
AU  - Pecarski, Danijela
AU  - Knežević-Jugović, Zorica
AU  - Dimitrijević-Branković, Suzana
AU  - Mihajilovski, Katarina
AU  - Janković, Slobodan
PY  - 2014
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2616
AB  - The aim of this study was to evaluate and compare the antimicrobial activity of four essential oils that belong to Lamiaceae family (sage, oregano, thyme) and eucalyptus oil. Gas chromatography coupled with mass spectrometry (GC-MS) revealed that the highest percent of essential oils 98.93% include three classes of compounds - monoterpene hydrocarbons, aromatic hydrocarbons and oxidized monoterpenes. The main components are oxidized monoterpenes: carvacrol (59.03%); thymol (36.12%), eucalyptol (20.66%), hydrocarbon monoterpenes: limonene (30.96%) and a-pinene (12.21%) and aromatic monoterpene, p-cymene (22.25%) All essential oils showed great potential of antimicrobial activity against several bacteria and yeast C. albicans, using the agar diffusion method with wells. Minimum inhibitory concentration (MIC) for the essential oils has been determined by the broth dilution method and valued in the range from 1 to 5 μL/m, depending on the essential oil and bacteria tested, and up to 100 μL/ml for C. albicans. The essential oils of Lamiaceae family exhibited a strong antibacterial activity for tested microorganisms, while the essential oils of thyme were especially recognized.
AB  - Ova studija se bavi ispitivanjem i upoređivanjem antimikrobnog dejstva etarskih ulja familije Lamiaceae: timijana, žalfije, origana i etarskog ulja eukaliptusa. Gasnom htomatografijom sa masenom spektrometrijom (GC-MS) je određena hemijska struktura i zastupljenost najvažnijih komponenata, pri čemu 98,92% sadržaja etarskog ulja zauzimaju tri klase jedinjenja, ugljovodonični monoterpeni, aromatični ugljovodonici i oksidovani monoterpeni, dok ostalim grupama jedinjena pripada manje od 1% sadržaja. Glavne komponenete ispitanih etarskih ulja su oksidovani monoterpeni: karvakrol (59,03%), timol (36,12%), eukaliptol (20,66%), i ugljovodonični monoterpeni: limonen (30,96%), a-pinen (12,21%), kao i aromatični monoterpen p-cimen (22,25%). Sva etarska ulja su pokazala značajnu anitimikrobnu aktivnst protiv svih testiranih bakterija i C.albicans, metodom difuzije u bunarčićima na agarnoj podlozi. Minimalna inhibitorna koncentracija je određena dilucionom metodom, i utvrđeno je da se vrednosti za MIC kreću u opsegu od 1μL/ml - 5 μL/ml za testirane bakterije, zavisno od ispitivanog etarskog ulja i bakterije koja se testira, i do 100 μL/ml za C. albicans. Etarska ulja familije Lamiaceae pokazuju snažno antimikrobno dejstvo, među kojima se posebno izdvaja etarsko ulje timijana.
PB  - Faculty of Technology, Novi Sad
T2  - Acta periodica technologica
T1  - Comparative analysis of the chemical composition and antimicrobal activities of some of Lamiaceae family species and eucaliptus (Eucaliptus globules M)
T1  - Komparativna analiza hemijskog sastava i antimikrobne aktivnosti nekih etarskih ulja familije Lamiaceae i etarskog ulja eukaliptusa (Eucaliptus globules M)
EP  - 213
IS  - 45
SP  - 201
DO  - 10.2298/APT1445201P
ER  - 

@article{
author = "Pecarski, Danijela and Knežević-Jugović, Zorica and Dimitrijević-Branković, Suzana and Mihajilovski, Katarina and Janković, Slobodan",
year = "2014",
abstract = "The aim of this study was to evaluate and compare the antimicrobial activity of four essential oils that belong to Lamiaceae family (sage, oregano, thyme) and eucalyptus oil. Gas chromatography coupled with mass spectrometry (GC-MS) revealed that the highest percent of essential oils 98.93% include three classes of compounds - monoterpene hydrocarbons, aromatic hydrocarbons and oxidized monoterpenes. The main components are oxidized monoterpenes: carvacrol (59.03%); thymol (36.12%), eucalyptol (20.66%), hydrocarbon monoterpenes: limonene (30.96%) and a-pinene (12.21%) and aromatic monoterpene, p-cymene (22.25%) All essential oils showed great potential of antimicrobial activity against several bacteria and yeast C. albicans, using the agar diffusion method with wells. Minimum inhibitory concentration (MIC) for the essential oils has been determined by the broth dilution method and valued in the range from 1 to 5 μL/m, depending on the essential oil and bacteria tested, and up to 100 μL/ml for C. albicans. The essential oils of Lamiaceae family exhibited a strong antibacterial activity for tested microorganisms, while the essential oils of thyme were especially recognized., Ova studija se bavi ispitivanjem i upoređivanjem antimikrobnog dejstva etarskih ulja familije Lamiaceae: timijana, žalfije, origana i etarskog ulja eukaliptusa. Gasnom htomatografijom sa masenom spektrometrijom (GC-MS) je određena hemijska struktura i zastupljenost najvažnijih komponenata, pri čemu 98,92% sadržaja etarskog ulja zauzimaju tri klase jedinjenja, ugljovodonični monoterpeni, aromatični ugljovodonici i oksidovani monoterpeni, dok ostalim grupama jedinjena pripada manje od 1% sadržaja. Glavne komponenete ispitanih etarskih ulja su oksidovani monoterpeni: karvakrol (59,03%), timol (36,12%), eukaliptol (20,66%), i ugljovodonični monoterpeni: limonen (30,96%), a-pinen (12,21%), kao i aromatični monoterpen p-cimen (22,25%). Sva etarska ulja su pokazala značajnu anitimikrobnu aktivnst protiv svih testiranih bakterija i C.albicans, metodom difuzije u bunarčićima na agarnoj podlozi. Minimalna inhibitorna koncentracija je određena dilucionom metodom, i utvrđeno je da se vrednosti za MIC kreću u opsegu od 1μL/ml - 5 μL/ml za testirane bakterije, zavisno od ispitivanog etarskog ulja i bakterije koja se testira, i do 100 μL/ml za C. albicans. Etarska ulja familije Lamiaceae pokazuju snažno antimikrobno dejstvo, među kojima se posebno izdvaja etarsko ulje timijana.",
publisher = "Faculty of Technology, Novi Sad",
journal = "Acta periodica technologica",
title = "Comparative analysis of the chemical composition and antimicrobal activities of some of Lamiaceae family species and eucaliptus (Eucaliptus globules M), Komparativna analiza hemijskog sastava i antimikrobne aktivnosti nekih etarskih ulja familije Lamiaceae i etarskog ulja eukaliptusa (Eucaliptus globules M)",
pages = "213-201",
number = "45",
doi = "10.2298/APT1445201P"
}

Pecarski, D., Knežević-Jugović, Z., Dimitrijević-Branković, S., Mihajilovski, K.,& Janković, S.. (2014). Comparative analysis of the chemical composition and antimicrobal activities of some of Lamiaceae family species and eucaliptus (Eucaliptus globules M). in Acta periodica technologica
Faculty of Technology, Novi Sad.(45), 201-213.
https://doi.org/10.2298/APT1445201P

Pecarski D, Knežević-Jugović Z, Dimitrijević-Branković S, Mihajilovski K, Janković S. Comparative analysis of the chemical composition and antimicrobal activities of some of Lamiaceae family species and eucaliptus (Eucaliptus globules M). in Acta periodica technologica. 2014;(45):201-213.
doi:10.2298/APT1445201P .

Pecarski, Danijela, Knežević-Jugović, Zorica, Dimitrijević-Branković, Suzana, Mihajilovski, Katarina, Janković, Slobodan, "Comparative analysis of the chemical composition and antimicrobal activities of some of Lamiaceae family species and eucaliptus (Eucaliptus globules M)" in Acta periodica technologica, no. 45 (2014):201-213,
https://doi.org/10.2298/APT1445201P . .

TY  - JOUR
AU  - Pecarski, Danijela
AU  - Knežević-Jugović, Zorica
AU  - Dimitrijević-Branković, Suzana
AU  - Mihajilovski, Katarina
AU  - Janković, Slobodan
PY  - 2014
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2643
AB  - Development of an available drug delivery system is of great interest considering the therapeutic effects of formulations with violate essential oils, especially assuming the fact that using essential oils as antimicrobial agents is a rather expanded in antimicrobial therapy nowadays. In this paper, the chitosan microparticles with encapsulated thyme essential oil were prepared in the emulsion by cross-linking method. The effect of thyme oil and glutaraldehyde initial concentrations on particle size, morphology, and particle size distribution was investigated. In addition, the influence of these parameters on the encapsulation of thyme oil in chitosan microparticles, concerning thyme oil loadings and encapsulation efficiency was also tested. The particles showed a spherical shape with an average diameter of 4.71±1.42 to 13.65±4.34 μm, depending on the concentration of the essential oil and glutaraldehyde that were used. The diameter of microparticles appeared to increase with increasing the thyme essential oil concentration, and decreased with the increase of glutaraldehyde concentration. It was shown that the concentration of glutaraldehyde did not affect the degree of encapsulation, but the increase in the initial concentration of thyme oil increased the degree of encapsulation of this essential oil in chitosan microparticles. All particles containing thyme essential oil, as well as essential oil of thyme itself showed significant antimicrobial activity against Staphylococcus aureus ATCC 25923, Esherichia coli ATCC 25922, Candida albicans ATCC 24433 and Enterococcus faecalis ATCC 25929. This study showed a great potential of the use of thyme essential oil as an antimicrobial agent, especially when encapsulated in a drug delivery system with controlled release of the active antimicrobial component.
AB  - Etarska ulja kao antimikrobni agensi su sve više u upoterbi u antimikrobnoj terapiji, pa je i razvoj odgovarajućeg drug delivery sistema sa etarskim uljem od velikog terapijskog značaja. Inkapsulacija isparljivih etrskih ulja u odgovarajuće čestice je od velikog značaja da bi se žaštitile bioaktivne komponente, koje su sastavni deo etarskih ulja i koje su osetljive na direktno delovanje svetlosti, temperature i oksidacije jer ovi faktori menjaju njihove biohemijske osobine. Hitozan je biodegradibilan, biokompatibilan, mukoadhezivan i netoksičan prirodni polimer koji kao takav ima ogroman potencijal kao nosač aktivnih supstanci u farmaceutskim formulacijama. Hitozanske mikročestice sa inkapsuliranim etarskim uljem timijana su izrađene emulzionom metodom uz primenu urežavajućeg agensa. Varirane su koncentracije etarskog ulja timijana i glutaraldehida kao umreživača, i praćen je njhov uticaj na veličinu čestica, količinu inkapsuliranog timijana i efikasnost inkapsulacije. Hitozanske mikročestice su imale sferičan oblik sa prosečnim prečnikom koji je bio u opsegu od 4.71±1.42 to 13.65±4.34 μm, u zavisnosti od koncentracije etarskog ulja i glutaraldehida koji su korišćeni pri izradi čestica. Prečnik čestica, odnosno veličina čestica, je rasla sa povećanjem koncentracije etarskog ulja timijana i smanjivala se sa povećanjem koncentracije glutaraldehida. Pokazano je da koncentracija glutaraldehida nema uticaja na stepen inkapsulacije etarskog ulja timijana u hitozanske mikročestice, dok sa porastom koncentracije etarskog ulja timijana raste količina inkapsuliranog ulja i efikasnost inkapsulacije. Sve mikročestice, uključujući i samo etarsko ulje timijana, su pokazali značajnu antimikrobnu aktivnost protiv Staphylococcus aureus ATCC 25923, Esherichia coli ATCC 25922, Candida albicans ATCC 24433 i Enterococcus faecalis ATCC 25929. Ova studija je pokazala veliki antimikrobni potencijal etarskog ulja timijana inkorporiranog u hitozanske mikročestice kao odgovarajuću farmaceutsku formulaciju sa kontrolisanim oslobađanjem aktivne antimikrobne komponente.
PB  - Association of Chemical Engineers of Serbia
T2  - Hemijska industrija
T1  - Preparation, characterization and antimicrobial activity of chitosan microparticles with thyme essential oil
T1  - Izrada, karakterizacija i antimikrobna aktivnost hitozanskih mikročestica sa etarskim uljem timijana
EP  - 729
IS  - 6
SP  - 721
VL  - 68
DO  - 10.2298/HEMIND140415048P
ER  - 

@article{
author = "Pecarski, Danijela and Knežević-Jugović, Zorica and Dimitrijević-Branković, Suzana and Mihajilovski, Katarina and Janković, Slobodan",
year = "2014",
abstract = "Development of an available drug delivery system is of great interest considering the therapeutic effects of formulations with violate essential oils, especially assuming the fact that using essential oils as antimicrobial agents is a rather expanded in antimicrobial therapy nowadays. In this paper, the chitosan microparticles with encapsulated thyme essential oil were prepared in the emulsion by cross-linking method. The effect of thyme oil and glutaraldehyde initial concentrations on particle size, morphology, and particle size distribution was investigated. In addition, the influence of these parameters on the encapsulation of thyme oil in chitosan microparticles, concerning thyme oil loadings and encapsulation efficiency was also tested. The particles showed a spherical shape with an average diameter of 4.71±1.42 to 13.65±4.34 μm, depending on the concentration of the essential oil and glutaraldehyde that were used. The diameter of microparticles appeared to increase with increasing the thyme essential oil concentration, and decreased with the increase of glutaraldehyde concentration. It was shown that the concentration of glutaraldehyde did not affect the degree of encapsulation, but the increase in the initial concentration of thyme oil increased the degree of encapsulation of this essential oil in chitosan microparticles. All particles containing thyme essential oil, as well as essential oil of thyme itself showed significant antimicrobial activity against Staphylococcus aureus ATCC 25923, Esherichia coli ATCC 25922, Candida albicans ATCC 24433 and Enterococcus faecalis ATCC 25929. This study showed a great potential of the use of thyme essential oil as an antimicrobial agent, especially when encapsulated in a drug delivery system with controlled release of the active antimicrobial component., Etarska ulja kao antimikrobni agensi su sve više u upoterbi u antimikrobnoj terapiji, pa je i razvoj odgovarajućeg drug delivery sistema sa etarskim uljem od velikog terapijskog značaja. Inkapsulacija isparljivih etrskih ulja u odgovarajuće čestice je od velikog značaja da bi se žaštitile bioaktivne komponente, koje su sastavni deo etarskih ulja i koje su osetljive na direktno delovanje svetlosti, temperature i oksidacije jer ovi faktori menjaju njihove biohemijske osobine. Hitozan je biodegradibilan, biokompatibilan, mukoadhezivan i netoksičan prirodni polimer koji kao takav ima ogroman potencijal kao nosač aktivnih supstanci u farmaceutskim formulacijama. Hitozanske mikročestice sa inkapsuliranim etarskim uljem timijana su izrađene emulzionom metodom uz primenu urežavajućeg agensa. Varirane su koncentracije etarskog ulja timijana i glutaraldehida kao umreživača, i praćen je njhov uticaj na veličinu čestica, količinu inkapsuliranog timijana i efikasnost inkapsulacije. Hitozanske mikročestice su imale sferičan oblik sa prosečnim prečnikom koji je bio u opsegu od 4.71±1.42 to 13.65±4.34 μm, u zavisnosti od koncentracije etarskog ulja i glutaraldehida koji su korišćeni pri izradi čestica. Prečnik čestica, odnosno veličina čestica, je rasla sa povećanjem koncentracije etarskog ulja timijana i smanjivala se sa povećanjem koncentracije glutaraldehida. Pokazano je da koncentracija glutaraldehida nema uticaja na stepen inkapsulacije etarskog ulja timijana u hitozanske mikročestice, dok sa porastom koncentracije etarskog ulja timijana raste količina inkapsuliranog ulja i efikasnost inkapsulacije. Sve mikročestice, uključujući i samo etarsko ulje timijana, su pokazali značajnu antimikrobnu aktivnost protiv Staphylococcus aureus ATCC 25923, Esherichia coli ATCC 25922, Candida albicans ATCC 24433 i Enterococcus faecalis ATCC 25929. Ova studija je pokazala veliki antimikrobni potencijal etarskog ulja timijana inkorporiranog u hitozanske mikročestice kao odgovarajuću farmaceutsku formulaciju sa kontrolisanim oslobađanjem aktivne antimikrobne komponente.",
publisher = "Association of Chemical Engineers of Serbia",
journal = "Hemijska industrija",
title = "Preparation, characterization and antimicrobial activity of chitosan microparticles with thyme essential oil, Izrada, karakterizacija i antimikrobna aktivnost hitozanskih mikročestica sa etarskim uljem timijana",
pages = "729-721",
number = "6",
volume = "68",
doi = "10.2298/HEMIND140415048P"
}

Pecarski, D., Knežević-Jugović, Z., Dimitrijević-Branković, S., Mihajilovski, K.,& Janković, S.. (2014). Preparation, characterization and antimicrobial activity of chitosan microparticles with thyme essential oil. in Hemijska industrija
Association of Chemical Engineers of Serbia., 68(6), 721-729.
https://doi.org/10.2298/HEMIND140415048P

Pecarski D, Knežević-Jugović Z, Dimitrijević-Branković S, Mihajilovski K, Janković S. Preparation, characterization and antimicrobial activity of chitosan microparticles with thyme essential oil. in Hemijska industrija. 2014;68(6):721-729.
doi:10.2298/HEMIND140415048P .

Pecarski, Danijela, Knežević-Jugović, Zorica, Dimitrijević-Branković, Suzana, Mihajilovski, Katarina, Janković, Slobodan, "Preparation, characterization and antimicrobial activity of chitosan microparticles with thyme essential oil" in Hemijska industrija, 68, no. 6 (2014):721-729,
https://doi.org/10.2298/HEMIND140415048P . .