TY  - JOUR
AU  - Dimitrijević, Snežana
AU  - Milić, Marija
AU  - Tadić, Vanja
AU  - Maksimović, Svetolik
AU  - Filipović, Vladimir
AU  - Dimitrijević-Branković, Suzana
AU  - Miljković, Miona
AU  - Salamon, Ivan
PY  - 2024-02
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7036
AB  - Within this investigation, the effect of conventional and sophisticated extraction method was evaluated on the chemical composition of black cumin seeds (Nigella sativa L.) essential oils and their bioactive characteristics. Soxhlet extraction at 60 °C by hexane (SEH) and petroleum ether (SEP) was adopted as traditionally used technique, while the supercritical extraction with CO2 (SFE), under the pressure of 10 MPa and temperature of 40 °C, was performed as a modern method, expected to overcome the drawbacks of the conventional solvent extraction. The results showed that SFE enabled up to ten times higher extraction yields, compared to essential oils derived after Soxhlet extraction, however, the bioactive compounds content, such as polyphenols, flavonoids and carotenoids, were extracted in higher quantities in the oil from Soxhlet extraction performance. Thereby, the strongest antioxidant agent was found to be the essential oil obtained from SEP, followed by the oil from SFE and oil obtained from SEH, respectively. The GC-MS analysis revealed that the content of thymoquinone was the highest in the essential oil from SEP (69.58%), and the obtained values are among the highest reported within the available literature data. Also, this oil sample contained about eleven times higher thymoquinone quantities than the essential oil obtained by SFE, which was mostly constituted of a methyl linoleate, a fatty acid methyl ester of linoleic acid. In addition, the essential oil from SEP exerted a value added antimicrobial properties, where it was found to be particularly effective in suppression of the Gram-positive bacteria growth.
PB  - Elsevier B.V.
T2  - Sustainable Chemistry and Pharmacy
T1  - Black cumin essential oil as a valuable source of bioactive compounds: Evaluation of the conventional vs. modern extraction technique
SP  - 101390
VL  - 37
DO  - 10.1016/j.scp.2023.101390
ER  - 

@article{
author = "Dimitrijević, Snežana and Milić, Marija and Tadić, Vanja and Maksimović, Svetolik and Filipović, Vladimir and Dimitrijević-Branković, Suzana and Miljković, Miona and Salamon, Ivan",
year = "2024-02",
abstract = "Within this investigation, the effect of conventional and sophisticated extraction method was evaluated on the chemical composition of black cumin seeds (Nigella sativa L.) essential oils and their bioactive characteristics. Soxhlet extraction at 60 °C by hexane (SEH) and petroleum ether (SEP) was adopted as traditionally used technique, while the supercritical extraction with CO2 (SFE), under the pressure of 10 MPa and temperature of 40 °C, was performed as a modern method, expected to overcome the drawbacks of the conventional solvent extraction. The results showed that SFE enabled up to ten times higher extraction yields, compared to essential oils derived after Soxhlet extraction, however, the bioactive compounds content, such as polyphenols, flavonoids and carotenoids, were extracted in higher quantities in the oil from Soxhlet extraction performance. Thereby, the strongest antioxidant agent was found to be the essential oil obtained from SEP, followed by the oil from SFE and oil obtained from SEH, respectively. The GC-MS analysis revealed that the content of thymoquinone was the highest in the essential oil from SEP (69.58%), and the obtained values are among the highest reported within the available literature data. Also, this oil sample contained about eleven times higher thymoquinone quantities than the essential oil obtained by SFE, which was mostly constituted of a methyl linoleate, a fatty acid methyl ester of linoleic acid. In addition, the essential oil from SEP exerted a value added antimicrobial properties, where it was found to be particularly effective in suppression of the Gram-positive bacteria growth.",
publisher = "Elsevier B.V.",
journal = "Sustainable Chemistry and Pharmacy",
title = "Black cumin essential oil as a valuable source of bioactive compounds: Evaluation of the conventional vs. modern extraction technique",
pages = "101390",
volume = "37",
doi = "10.1016/j.scp.2023.101390"
}

Dimitrijević, S., Milić, M., Tadić, V., Maksimović, S., Filipović, V., Dimitrijević-Branković, S., Miljković, M.,& Salamon, I.. (2024-02). Black cumin essential oil as a valuable source of bioactive compounds: Evaluation of the conventional vs. modern extraction technique. in Sustainable Chemistry and Pharmacy
Elsevier B.V.., 37, 101390.
https://doi.org/10.1016/j.scp.2023.101390

Dimitrijević S, Milić M, Tadić V, Maksimović S, Filipović V, Dimitrijević-Branković S, Miljković M, Salamon I. Black cumin essential oil as a valuable source of bioactive compounds: Evaluation of the conventional vs. modern extraction technique. in Sustainable Chemistry and Pharmacy. 2024;37:101390.
doi:10.1016/j.scp.2023.101390 .

Dimitrijević, Snežana, Milić, Marija, Tadić, Vanja, Maksimović, Svetolik, Filipović, Vladimir, Dimitrijević-Branković, Suzana, Miljković, Miona, Salamon, Ivan, "Black cumin essential oil as a valuable source of bioactive compounds: Evaluation of the conventional vs. modern extraction technique" in Sustainable Chemistry and Pharmacy, 37 (2024-02):101390,
https://doi.org/10.1016/j.scp.2023.101390 . .

TY  - CONF
AU  - Ilić, Nevena
AU  - Davidović, Slađana
AU  - Miljković, Miona
AU  - Radovanović, Neda
AU  - Dimitrijević-Branković, Suzana
AU  - Mihajlovski, Katarina
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6943
AB  - In recent decades, enzyme immobilization on different supports occupies an important place in the modern biotechnology, given that it allows the design of green and sustainable production processes. Nanoparticles are very efficient supports for enzyme immobilization. The enzyme immobilized on nanoparticles is more stable than its soluble form and can be used in several operative cycles. In this study, the potential of modified titanium dioxide nanoparticles (Degussa P25, TiO2) for crude fungal laccase (47.43 U/g) immobilization was investigated. The TiO2 nanoparticles were modified with (3-glycidyloxypropyl) trimetoxylane under different conditions in order to obtain support with an optimal concentration of epoxy groups for immobilization. The obtained TiO2 nanoparticles had different concentrations of epoxy groups on their surface, exactly 950 μmol/g (TiO2M1) and 500 μmol/g (TiO2M2), respectively. The immobilization was carried out at pH 5 and at room temperature for 4 h. The residual activity of immobilized laccase on TiO2M2 was 33.40 %, while the residual activity of immobilized laccase on TiO2M1 was 17.39 %. The immobilization efficiency was 26.75 % and 20.18 % for laccase immobilized on TiO2M2 and immobilized laccase on TiO2M1, respectively. The immobilization of crude fungal laccase on TiO2M2 was further optimized by testing the influence of different contact time between laccase and TiO2M2 (2 h ,3 h, 3,5 h and 4 h) as well as the effect of various pH values (pH 4, pH 5 and pH 6) on immobilization. The highest residual activity of 35.04 % and immobilization efficiency of 28.95 % were obtained for optimal contact time of 3.5 h between crude fungal laccase and TiO2M2. The lowest residual activity (30.38 %) and immobilization efficiency (20.18 %) were obtained when immobilization was carried out at room temperature and at pH 5 for 2 h. The pH values had significant effect on immobilization. The optimal pH value was pH 5 with highest residual activity of 35.04 %, while the lowest residual activity of 14. 83 % was at pH 6. The residual activity of 21.21 % and immobilization efficency of 19.52 % were obtained when crude fungal laccase immobilized at pH 4. The immobilized laccase on TiO2M2 was successfully used in 5 cycles of guaiacol oxidation. In the 2nd cycle, the immobilized laccase had residual activity of about 60%, while after 3rd cycle, immobilized laccase had residual activity of 40 %. The lowest residual activity of 14.83 %, immobilized laccase had after 5th cycle.
PB  - Zvornik : University of East Sarajevo Faculty of Technology
C3  - Proceedings / VIII International Congress "Engineering, Environment and Materials in Process Industry", EEM2023, Jahorina, March 20-23, 2023
T1  - IMMOBILIZATION OF CRUDE FUNGAL LACCASE FROM GANODERMA SPP. ON MODIFIED TITANIUM DIOXIDE NANOPARTICLES
EP  - 196
SP  - 187
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6943
ER  - 

@conference{
author = "Ilić, Nevena and Davidović, Slađana and Miljković, Miona and Radovanović, Neda and Dimitrijević-Branković, Suzana and Mihajlovski, Katarina",
year = "2023",
abstract = "In recent decades, enzyme immobilization on different supports occupies an important place in the modern biotechnology, given that it allows the design of green and sustainable production processes. Nanoparticles are very efficient supports for enzyme immobilization. The enzyme immobilized on nanoparticles is more stable than its soluble form and can be used in several operative cycles. In this study, the potential of modified titanium dioxide nanoparticles (Degussa P25, TiO2) for crude fungal laccase (47.43 U/g) immobilization was investigated. The TiO2 nanoparticles were modified with (3-glycidyloxypropyl) trimetoxylane under different conditions in order to obtain support with an optimal concentration of epoxy groups for immobilization. The obtained TiO2 nanoparticles had different concentrations of epoxy groups on their surface, exactly 950 μmol/g (TiO2M1) and 500 μmol/g (TiO2M2), respectively. The immobilization was carried out at pH 5 and at room temperature for 4 h. The residual activity of immobilized laccase on TiO2M2 was 33.40 %, while the residual activity of immobilized laccase on TiO2M1 was 17.39 %. The immobilization efficiency was 26.75 % and 20.18 % for laccase immobilized on TiO2M2 and immobilized laccase on TiO2M1, respectively. The immobilization of crude fungal laccase on TiO2M2 was further optimized by testing the influence of different contact time between laccase and TiO2M2 (2 h ,3 h, 3,5 h and 4 h) as well as the effect of various pH values (pH 4, pH 5 and pH 6) on immobilization. The highest residual activity of 35.04 % and immobilization efficiency of 28.95 % were obtained for optimal contact time of 3.5 h between crude fungal laccase and TiO2M2. The lowest residual activity (30.38 %) and immobilization efficiency (20.18 %) were obtained when immobilization was carried out at room temperature and at pH 5 for 2 h. The pH values had significant effect on immobilization. The optimal pH value was pH 5 with highest residual activity of 35.04 %, while the lowest residual activity of 14. 83 % was at pH 6. The residual activity of 21.21 % and immobilization efficency of 19.52 % were obtained when crude fungal laccase immobilized at pH 4. The immobilized laccase on TiO2M2 was successfully used in 5 cycles of guaiacol oxidation. In the 2nd cycle, the immobilized laccase had residual activity of about 60%, while after 3rd cycle, immobilized laccase had residual activity of 40 %. The lowest residual activity of 14.83 %, immobilized laccase had after 5th cycle.",
publisher = "Zvornik : University of East Sarajevo Faculty of Technology",
journal = "Proceedings / VIII International Congress "Engineering, Environment and Materials in Process Industry", EEM2023, Jahorina, March 20-23, 2023",
title = "IMMOBILIZATION OF CRUDE FUNGAL LACCASE FROM GANODERMA SPP. ON MODIFIED TITANIUM DIOXIDE NANOPARTICLES",
pages = "196-187",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6943"
}

Ilić, N., Davidović, S., Miljković, M., Radovanović, N., Dimitrijević-Branković, S.,& Mihajlovski, K.. (2023). IMMOBILIZATION OF CRUDE FUNGAL LACCASE FROM GANODERMA SPP. ON MODIFIED TITANIUM DIOXIDE NANOPARTICLES. in Proceedings / VIII International Congress "Engineering, Environment and Materials in Process Industry", EEM2023, Jahorina, March 20-23, 2023
Zvornik : University of East Sarajevo Faculty of Technology., 187-196.
https://hdl.handle.net/21.15107/rcub_technorep_6943

Ilić N, Davidović S, Miljković M, Radovanović N, Dimitrijević-Branković S, Mihajlovski K. IMMOBILIZATION OF CRUDE FUNGAL LACCASE FROM GANODERMA SPP. ON MODIFIED TITANIUM DIOXIDE NANOPARTICLES. in Proceedings / VIII International Congress "Engineering, Environment and Materials in Process Industry", EEM2023, Jahorina, March 20-23, 2023. 2023;:187-196.
https://hdl.handle.net/21.15107/rcub_technorep_6943 .

Ilić, Nevena, Davidović, Slađana, Miljković, Miona, Radovanović, Neda, Dimitrijević-Branković, Suzana, Mihajlovski, Katarina, "IMMOBILIZATION OF CRUDE FUNGAL LACCASE FROM GANODERMA SPP. ON MODIFIED TITANIUM DIOXIDE NANOPARTICLES" in Proceedings / VIII International Congress "Engineering, Environment and Materials in Process Industry", EEM2023, Jahorina, March 20-23, 2023 (2023):187-196,
https://hdl.handle.net/21.15107/rcub_technorep_6943 .

TY  - CONF
AU  - Davidović, Slađana
AU  - Miljković, Miona
AU  - Dimitrijević-Branković, Suzana
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7021
AB  - The use of edible films and coatings made of natural materials such as
polysaccharides provides a sustainable technological alternative to traditional plastic food
packaging materials as one of the contemporary methods for preserving food items and
guaranteeing their quality and freshness throughout their shelf lives. This is especially
important for fresh fruits as highly perishable.
The aim of the present study was to investigate the effect of dextran-based coating on
strawberry (Fragaria × ananassa) quality attributes during refrigerated storage. Dextran was
synthesized using dextransucrase from Leuconostoc mesenteroides T3. Coating solution was
made by dissolving 12g of dextran in 300 mL distilled water. Glycerol (25% w/w) and Tween
80 (5% w/w) were added as plasticizer and surfactant, respectively. Strawberries were coated
by dipping in coating solution or water (control) and after drying were placed in plastic trays
and stored at 8 °C. The influence of coating on strawberry quality was assessed through
measuring the percentage of weight loss (WL), total soluble solids (TSS) and titratable
acidity (TA) of the coated fruits and compared with the control uncoated samples.
The percentage of WL increased during storage in both coated and uncoated
strawberries and although it was less for the uncoated strawberries, the difference was not
significant. The TSS content was lower in dextran-coated fruit during the entire storage
period. Since TSS is an indicator of the ripeness of the fruit it could be concluded that dextran
coating slows down the ripening, thus increasing the shelf life. Unexpectedly, coated
strawberries exhibited lower TA values. However, the relative changes were significantly
smaller among the coated samples group, implying that the coating slows down the
respiration process in which the acids are consumed.
Overall, the results of this work show that the dextran coating has the potential to
preserve quality of strawberries during cold storage.
PB  - Belgrade : University, Faculty of Technology and Metallurgy
C3  - Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade
T1  - EFFECT OF DEXTRAN COATING ON STRAWBERRY QUALITY DURING REFRIGERATED STORAGE
SP  - 83
UR  - https://hdl.handle.net/21.15107/rcub_technorep_7021
ER  - 

@conference{
author = "Davidović, Slađana and Miljković, Miona and Dimitrijević-Branković, Suzana",
year = "2023",
abstract = "The use of edible films and coatings made of natural materials such as
polysaccharides provides a sustainable technological alternative to traditional plastic food
packaging materials as one of the contemporary methods for preserving food items and
guaranteeing their quality and freshness throughout their shelf lives. This is especially
important for fresh fruits as highly perishable.
The aim of the present study was to investigate the effect of dextran-based coating on
strawberry (Fragaria × ananassa) quality attributes during refrigerated storage. Dextran was
synthesized using dextransucrase from Leuconostoc mesenteroides T3. Coating solution was
made by dissolving 12g of dextran in 300 mL distilled water. Glycerol (25% w/w) and Tween
80 (5% w/w) were added as plasticizer and surfactant, respectively. Strawberries were coated
by dipping in coating solution or water (control) and after drying were placed in plastic trays
and stored at 8 °C. The influence of coating on strawberry quality was assessed through
measuring the percentage of weight loss (WL), total soluble solids (TSS) and titratable
acidity (TA) of the coated fruits and compared with the control uncoated samples.
The percentage of WL increased during storage in both coated and uncoated
strawberries and although it was less for the uncoated strawberries, the difference was not
significant. The TSS content was lower in dextran-coated fruit during the entire storage
period. Since TSS is an indicator of the ripeness of the fruit it could be concluded that dextran
coating slows down the ripening, thus increasing the shelf life. Unexpectedly, coated
strawberries exhibited lower TA values. However, the relative changes were significantly
smaller among the coated samples group, implying that the coating slows down the
respiration process in which the acids are consumed.
Overall, the results of this work show that the dextran coating has the potential to
preserve quality of strawberries during cold storage.",
publisher = "Belgrade : University, Faculty of Technology and Metallurgy",
journal = "Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade",
title = "EFFECT OF DEXTRAN COATING ON STRAWBERRY QUALITY DURING REFRIGERATED STORAGE",
pages = "83",
url = "https://hdl.handle.net/21.15107/rcub_technorep_7021"
}

Davidović, S., Miljković, M.,& Dimitrijević-Branković, S.. (2023). EFFECT OF DEXTRAN COATING ON STRAWBERRY QUALITY DURING REFRIGERATED STORAGE. in Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade
Belgrade : University, Faculty of Technology and Metallurgy., 83.
https://hdl.handle.net/21.15107/rcub_technorep_7021

Davidović S, Miljković M, Dimitrijević-Branković S. EFFECT OF DEXTRAN COATING ON STRAWBERRY QUALITY DURING REFRIGERATED STORAGE. in Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade. 2023;:83.
https://hdl.handle.net/21.15107/rcub_technorep_7021 .

Davidović, Slađana, Miljković, Miona, Dimitrijević-Branković, Suzana, "EFFECT OF DEXTRAN COATING ON STRAWBERRY QUALITY DURING REFRIGERATED STORAGE" in Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade (2023):83,
https://hdl.handle.net/21.15107/rcub_technorep_7021 .

TY  - CONF
AU  - Davidović, Slađana
AU  - Miljković, Miona
AU  - Ilić, Nevena
AU  - Milošević, Jelena
AU  - Dimitrijević-Branković, Suzana
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7030
AB  - By-products of soybean oil production are high-quality protein supplements for
animal feed. However, they are rich in anti-nutritive factors and indigestible components,
among which the special attention is focused on galactooligosaccharides, due to lack of αgalactosidase in monogastric animals. Enzymatic treatment of soy-based products and
microbial fermentation are employed for overcoming these issues.
The main goal of this study was to apply fermentation with a selected strain of black
yeast-like fungus (Aureobasidium spp.) of the soybean products in order to obtain highprotein soy-based animal feed. First, screening for an appropriate strain of microorganism
among ten natural isolates from grapes has been performed. Keeping in mind complex
structure of soybean polysaccharides the first selection criterion was the ability of growth in
the presence of arabinose, xylose, galactose, mannose, raffinose, or soybean oil as sole
carbon source. More important, enzymatic activity of α-galactosidase was detected in new
isolates. The highest α-galactosidase producer was selected for cultivation on soybean meal.
The selected strain was characterized in terms of physiological and technological properties
and identified as Aureobasidium pullulans P8. In order to obtain high protein soybean meal, it
has been utilized in two types of fermentation, i.e. solid state (SSF) and submerged (SMF)
under varying conditions. Maximal protein content (61.11% based on dry weight) was
obtained after 5 days of SMF at 30 °C and 10% of dry matter, while SSF produced 57.78%
protein after 7 days of incubation at 30 °C with substrate contained 30% of dry matter.
Extracellular enzymatic activities of cellulase, pectinase, amylase, xylanase and αgalactosidase were detected in supernatant after SMF, indicating its potential reusability for
hydrolysis of new batch of soybean substrate.
This investigation revealed the versatile extracellular enzymatic potential of newly
isolated black yeast-like fungus Aureobasidium pullulans P8 and its potential for production
of high protein soybean meal.
PB  - Belgrade : University, Faculty of Technology and Metallurgy
C3  - Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade, 2023
T1  - VALORIZATION OF SOYBEAN MEAL FOR PRODUCTION OF HIGH PROTEIN ANIMAL FEED AND VALUE-ADDED PRODUCTS USING NEW STRAIN OF AUREOBASIDIUM PULLULANS
SP  - 84
UR  - https://hdl.handle.net/21.15107/rcub_technorep_7030
ER  - 

@conference{
author = "Davidović, Slađana and Miljković, Miona and Ilić, Nevena and Milošević, Jelena and Dimitrijević-Branković, Suzana",
year = "2023",
abstract = "By-products of soybean oil production are high-quality protein supplements for
animal feed. However, they are rich in anti-nutritive factors and indigestible components,
among which the special attention is focused on galactooligosaccharides, due to lack of αgalactosidase in monogastric animals. Enzymatic treatment of soy-based products and
microbial fermentation are employed for overcoming these issues.
The main goal of this study was to apply fermentation with a selected strain of black
yeast-like fungus (Aureobasidium spp.) of the soybean products in order to obtain highprotein soy-based animal feed. First, screening for an appropriate strain of microorganism
among ten natural isolates from grapes has been performed. Keeping in mind complex
structure of soybean polysaccharides the first selection criterion was the ability of growth in
the presence of arabinose, xylose, galactose, mannose, raffinose, or soybean oil as sole
carbon source. More important, enzymatic activity of α-galactosidase was detected in new
isolates. The highest α-galactosidase producer was selected for cultivation on soybean meal.
The selected strain was characterized in terms of physiological and technological properties
and identified as Aureobasidium pullulans P8. In order to obtain high protein soybean meal, it
has been utilized in two types of fermentation, i.e. solid state (SSF) and submerged (SMF)
under varying conditions. Maximal protein content (61.11% based on dry weight) was
obtained after 5 days of SMF at 30 °C and 10% of dry matter, while SSF produced 57.78%
protein after 7 days of incubation at 30 °C with substrate contained 30% of dry matter.
Extracellular enzymatic activities of cellulase, pectinase, amylase, xylanase and αgalactosidase were detected in supernatant after SMF, indicating its potential reusability for
hydrolysis of new batch of soybean substrate.
This investigation revealed the versatile extracellular enzymatic potential of newly
isolated black yeast-like fungus Aureobasidium pullulans P8 and its potential for production
of high protein soybean meal.",
publisher = "Belgrade : University, Faculty of Technology and Metallurgy",
journal = "Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade, 2023",
title = "VALORIZATION OF SOYBEAN MEAL FOR PRODUCTION OF HIGH PROTEIN ANIMAL FEED AND VALUE-ADDED PRODUCTS USING NEW STRAIN OF AUREOBASIDIUM PULLULANS",
pages = "84",
url = "https://hdl.handle.net/21.15107/rcub_technorep_7030"
}

Davidović, S., Miljković, M., Ilić, N., Milošević, J.,& Dimitrijević-Branković, S.. (2023). VALORIZATION OF SOYBEAN MEAL FOR PRODUCTION OF HIGH PROTEIN ANIMAL FEED AND VALUE-ADDED PRODUCTS USING NEW STRAIN OF AUREOBASIDIUM PULLULANS. in Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade, 2023
Belgrade : University, Faculty of Technology and Metallurgy., 84.
https://hdl.handle.net/21.15107/rcub_technorep_7030

Davidović S, Miljković M, Ilić N, Milošević J, Dimitrijević-Branković S. VALORIZATION OF SOYBEAN MEAL FOR PRODUCTION OF HIGH PROTEIN ANIMAL FEED AND VALUE-ADDED PRODUCTS USING NEW STRAIN OF AUREOBASIDIUM PULLULANS. in Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade, 2023. 2023;:84.
https://hdl.handle.net/21.15107/rcub_technorep_7030 .

Davidović, Slađana, Miljković, Miona, Ilić, Nevena, Milošević, Jelena, Dimitrijević-Branković, Suzana, "VALORIZATION OF SOYBEAN MEAL FOR PRODUCTION OF HIGH PROTEIN ANIMAL FEED AND VALUE-ADDED PRODUCTS USING NEW STRAIN OF AUREOBASIDIUM PULLULANS" in Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade, 2023 (2023):84,
https://hdl.handle.net/21.15107/rcub_technorep_7030 .

TY  - JOUR
AU  - Nešić, Aleksandra
AU  - Meseldžija, Slađana
AU  - Davidović, Slađana
AU  - Miljković, Miona
AU  - Onjia, Antonije
AU  - Dimitrijević, Suzana
AU  - Santagata, Gabriella
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6613
AB  - In this study, pectin-based films blended with alginate and crosslinked with copper ions for potential application in agriculture were investigated. Copper was selected as crosslinking agent, due to its key role as a plant micronutrient, antifungal ability, and focal activity in the pectin crosslinking process. FTIR analysis confirmed the interactions between pectin, alginate and copper salt. The inclusion of alginate in film improved the binding activity of copper ions, providing denser 3d network with enhanced physical-chemical properties. The crosslinked film containing 60 wt% of pectin and 40 wt% of alginate had the highest tensile strength (59.8 MPa), highest UV barrier (transmission of light=0%), and the lowest swelling degree (121%) and water vapor permeability (0.320 ng/ m s Pa). Moreover, the release of copper ions from this formulation after 28 days of immersion in water and soil was 9 and 13 ppm, respectively. The biodegradation rate after 28 days of exposure in the soil was 54%. Nevertheless, all crosslinked pectin-alginate films inhibited the growth of the Fusarium and Aspergillus phytopathogens and demonstrated non-phytotoxicity on germination and growth of wheat. Hence, the system developed in this work can potentially be used as novel, bio-active, tunable plant protective biodegradable foliar spray in agriculture sector.
PB  - Elsevier B.V.
T2  - Industrial Crops and Products
T1  - Characterization of antifungal citrus pectin-based films for potential agricultural application
SP  - 117386
VL  - 204
DO  - 10.1016/j.indcrop.2023.117386
ER  - 

@article{
author = "Nešić, Aleksandra and Meseldžija, Slađana and Davidović, Slađana and Miljković, Miona and Onjia, Antonije and Dimitrijević, Suzana and Santagata, Gabriella",
year = "2023",
abstract = "In this study, pectin-based films blended with alginate and crosslinked with copper ions for potential application in agriculture were investigated. Copper was selected as crosslinking agent, due to its key role as a plant micronutrient, antifungal ability, and focal activity in the pectin crosslinking process. FTIR analysis confirmed the interactions between pectin, alginate and copper salt. The inclusion of alginate in film improved the binding activity of copper ions, providing denser 3d network with enhanced physical-chemical properties. The crosslinked film containing 60 wt% of pectin and 40 wt% of alginate had the highest tensile strength (59.8 MPa), highest UV barrier (transmission of light=0%), and the lowest swelling degree (121%) and water vapor permeability (0.320 ng/ m s Pa). Moreover, the release of copper ions from this formulation after 28 days of immersion in water and soil was 9 and 13 ppm, respectively. The biodegradation rate after 28 days of exposure in the soil was 54%. Nevertheless, all crosslinked pectin-alginate films inhibited the growth of the Fusarium and Aspergillus phytopathogens and demonstrated non-phytotoxicity on germination and growth of wheat. Hence, the system developed in this work can potentially be used as novel, bio-active, tunable plant protective biodegradable foliar spray in agriculture sector.",
publisher = "Elsevier B.V.",
journal = "Industrial Crops and Products",
title = "Characterization of antifungal citrus pectin-based films for potential agricultural application",
pages = "117386",
volume = "204",
doi = "10.1016/j.indcrop.2023.117386"
}

Nešić, A., Meseldžija, S., Davidović, S., Miljković, M., Onjia, A., Dimitrijević, S.,& Santagata, G.. (2023). Characterization of antifungal citrus pectin-based films for potential agricultural application. in Industrial Crops and Products
Elsevier B.V.., 204, 117386.
https://doi.org/10.1016/j.indcrop.2023.117386

Nešić A, Meseldžija S, Davidović S, Miljković M, Onjia A, Dimitrijević S, Santagata G. Characterization of antifungal citrus pectin-based films for potential agricultural application. in Industrial Crops and Products. 2023;204:117386.
doi:10.1016/j.indcrop.2023.117386 .

Nešić, Aleksandra, Meseldžija, Slađana, Davidović, Slađana, Miljković, Miona, Onjia, Antonije, Dimitrijević, Suzana, Santagata, Gabriella, "Characterization of antifungal citrus pectin-based films for potential agricultural application" in Industrial Crops and Products, 204 (2023):117386,
https://doi.org/10.1016/j.indcrop.2023.117386 . .

TY  - CONF
AU  - Ilić, Nevena
AU  - Kukučka, Andrej
AU  - Milić, Marija
AU  - Milutinović, Milica
AU  - Miljković, Miona
AU  - Davidović, Slađana
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5938
AB  - Antimicrobial properties of silver nanoparticles (Ag NPs) are well known and widely exploited. Various approaches have been applied for preparation of Ag NPs. Nowadays, „green“ methods are attracting more attention since they are eco-friendly and generally cheap. Moreover, incorporation of Ag NPs into various natural polymers enables preparation of biocompatible antimicrobial materials with controlled releasing of Ag NPs. Polymeric material also provides long term stability of Ag NPs. The aim of this study was to develop antimicrobial materials based on silver nanoparticles using green approach. Reduction of silver was performed by plant extracts of horsetail (Equisetum arvense L.) and cocoa (Theobroma cacao); the obtained nanoparticles were designated as EA Ag NPs and TC Ag NPs, respectively. The effect of extract concentration on particle size distribution and antimicrobial activity against Gram-negative bacteria Escherichia coli and Gram-positive bacteria Staphylococcus aureus were investigated. The smallest particles with the narrowest size distribution (between 46 nm and 91 nm) were obtained with the lowest tested concentration (10% v/v) of plant extract for both horsetail and cacao. These samples also showed the highest antibacterial activities, so they were selected for preparation of nanocomposite films based on agar-agar and chitosan. It has been shown that incorporation of both EA and TC Ag NPs significantly improved antibacterial properties of the films against E. coli (≈80% higher inhibition in comparison to the pristine agar-agar/chitosan films). In the case of S. aures, TC Ag NPs incorporated in agar-agar/chitosan films enhanced inhibition of the film for 60%. On the other side, incorporation of EA Ag NPs into agar-agar/chitosan film increased the growth inhibition for only 20%. This might be due to the contribution of cocoa extract itself, i.e. some active components that inhibit the growth of S. aureus. Our results show that agar-agar/chitosan films with incorporated green synthesized Ag NPs have potential application in the areas where usage of biodegradable and biocompatible materials with high antibacterial activity is desired, such as food package, wound healing, coatings for medical devices etc.
C3  - Proceedings,VII International Congress “ Engineering, Environment and Materials in Process Industry
T1  - Synthesis and characterization of agar-agar-chitosan composite films incorporated with green synthesized silver nanoparticles
EP  - 469
SP  - 461
UR  - https://hdl.handle.net/21.15107/rcub_technorep_5938
ER  - 

@conference{
author = "Ilić, Nevena and Kukučka, Andrej and Milić, Marija and Milutinović, Milica and Miljković, Miona and Davidović, Slađana",
year = "2021",
abstract = "Antimicrobial properties of silver nanoparticles (Ag NPs) are well known and widely exploited. Various approaches have been applied for preparation of Ag NPs. Nowadays, „green“ methods are attracting more attention since they are eco-friendly and generally cheap. Moreover, incorporation of Ag NPs into various natural polymers enables preparation of biocompatible antimicrobial materials with controlled releasing of Ag NPs. Polymeric material also provides long term stability of Ag NPs. The aim of this study was to develop antimicrobial materials based on silver nanoparticles using green approach. Reduction of silver was performed by plant extracts of horsetail (Equisetum arvense L.) and cocoa (Theobroma cacao); the obtained nanoparticles were designated as EA Ag NPs and TC Ag NPs, respectively. The effect of extract concentration on particle size distribution and antimicrobial activity against Gram-negative bacteria Escherichia coli and Gram-positive bacteria Staphylococcus aureus were investigated. The smallest particles with the narrowest size distribution (between 46 nm and 91 nm) were obtained with the lowest tested concentration (10% v/v) of plant extract for both horsetail and cacao. These samples also showed the highest antibacterial activities, so they were selected for preparation of nanocomposite films based on agar-agar and chitosan. It has been shown that incorporation of both EA and TC Ag NPs significantly improved antibacterial properties of the films against E. coli (≈80% higher inhibition in comparison to the pristine agar-agar/chitosan films). In the case of S. aures, TC Ag NPs incorporated in agar-agar/chitosan films enhanced inhibition of the film for 60%. On the other side, incorporation of EA Ag NPs into agar-agar/chitosan film increased the growth inhibition for only 20%. This might be due to the contribution of cocoa extract itself, i.e. some active components that inhibit the growth of S. aureus. Our results show that agar-agar/chitosan films with incorporated green synthesized Ag NPs have potential application in the areas where usage of biodegradable and biocompatible materials with high antibacterial activity is desired, such as food package, wound healing, coatings for medical devices etc.",
journal = "Proceedings,VII International Congress “ Engineering, Environment and Materials in Process Industry",
title = "Synthesis and characterization of agar-agar-chitosan composite films incorporated with green synthesized silver nanoparticles",
pages = "469-461",
url = "https://hdl.handle.net/21.15107/rcub_technorep_5938"
}

Ilić, N., Kukučka, A., Milić, M., Milutinović, M., Miljković, M.,& Davidović, S.. (2021). Synthesis and characterization of agar-agar-chitosan composite films incorporated with green synthesized silver nanoparticles. in Proceedings,VII International Congress “ Engineering, Environment and Materials in Process Industry, 461-469.
https://hdl.handle.net/21.15107/rcub_technorep_5938

Ilić N, Kukučka A, Milić M, Milutinović M, Miljković M, Davidović S. Synthesis and characterization of agar-agar-chitosan composite films incorporated with green synthesized silver nanoparticles. in Proceedings,VII International Congress “ Engineering, Environment and Materials in Process Industry. 2021;:461-469.
https://hdl.handle.net/21.15107/rcub_technorep_5938 .

Ilić, Nevena, Kukučka, Andrej, Milić, Marija, Milutinović, Milica, Miljković, Miona, Davidović, Slađana, "Synthesis and characterization of agar-agar-chitosan composite films incorporated with green synthesized silver nanoparticles" in Proceedings,VII International Congress “ Engineering, Environment and Materials in Process Industry (2021):461-469,
https://hdl.handle.net/21.15107/rcub_technorep_5938 .

TY  - CONF
AU  - Ilić, Nevena
AU  - Lazić, Vesna
AU  - Radovanović, Neda
AU  - Mihajlovski, Katarina
AU  - Davidović, Slađana
AU  - Miljković, Miona
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5927
AB  - Silver and titanium dioxide nanoparticles (NPs) are the most produced NPs worldwide due to their antimicrobial properties. These NPs are widely used in many fields including agriculture, where they can inhibit the growth of plant pathogens and stimulate plant growth. However, there are concerns about nanoparticles reaching the environment, as they can also manifest adverse effects. In this study, the effects of TiO2 and two types of Ag NPs (synthesized by chemical reduction and “green” by dextrane) on soil microorganisms and organic beans were investigated. Two different species of rhizospheric soil microorganisms, namely actinomycetes Streptomyces microflavus and nitrogen-fixing soil bacteria Rhizobium sp. were exposed to the low concentration of NPs. For that purpose, microorganisms were grown in nutrient media supplemented with 0.2 mM NPs. After 24 h of exposure, the numbers of viable cells of both tested microorganisms were higher in the samples compared to the controls (media without NPs) for all three NPs, suggesting their stimulatory effect on the soil microorganisms' growth. The highest positive effect was observed for Rhizobium sp. treated with TiO2 NPs (the number of viable cells increased for two orders of magnitude, from ~106 to ~108 CFU/mL). Ag NPs showed equal influence on both tested microorganisms, with dextrane-coated Ag NPs were more effective. In the experiment with organic mung beans, the same colloid solutions of Ag NPs and TiO2 NPs had a stimulating effect on the germination and root length of this plant, also. The number of germinated seeds was the same as in the control, while the average root length was higher in the samples than in the control. The average root length was 1.47 cm in the control. Seeds treated with dextrane-coated Ag NPs had an average root length even 82 % higher compared to the control. The stimulatory effect was also observed in the case of seeds treated with Ag NPs synthesized by chemical reduction and TiO2 NPs, where the average root lengths were higher for 40% and 76 %, respectively than in the control. Germination index values, which are higher than 100%, suggesting that tested nanoparticles can be used as fertilizers in agriculture. Moreover, the obtained results show that silver nanoparticles and titanium dioxide nanoparticles have a stimulatory effect on the soil bacteria and organic mung bean, which prove their safety for this kind of application.
C3  - Proceedings,VII International Congress “ Engineering, Environment and Materials in Process Industry
T1  - Investigation of the influence of different nanoparticles on the growth of soil microorganisms and organic mung bean
EP  - 460
SP  - 451
UR  - https://hdl.handle.net/21.15107/rcub_technorep_5927
ER  - 

@conference{
author = "Ilić, Nevena and Lazić, Vesna and Radovanović, Neda and Mihajlovski, Katarina and Davidović, Slađana and Miljković, Miona",
year = "2021",
abstract = "Silver and titanium dioxide nanoparticles (NPs) are the most produced NPs worldwide due to their antimicrobial properties. These NPs are widely used in many fields including agriculture, where they can inhibit the growth of plant pathogens and stimulate plant growth. However, there are concerns about nanoparticles reaching the environment, as they can also manifest adverse effects. In this study, the effects of TiO2 and two types of Ag NPs (synthesized by chemical reduction and “green” by dextrane) on soil microorganisms and organic beans were investigated. Two different species of rhizospheric soil microorganisms, namely actinomycetes Streptomyces microflavus and nitrogen-fixing soil bacteria Rhizobium sp. were exposed to the low concentration of NPs. For that purpose, microorganisms were grown in nutrient media supplemented with 0.2 mM NPs. After 24 h of exposure, the numbers of viable cells of both tested microorganisms were higher in the samples compared to the controls (media without NPs) for all three NPs, suggesting their stimulatory effect on the soil microorganisms' growth. The highest positive effect was observed for Rhizobium sp. treated with TiO2 NPs (the number of viable cells increased for two orders of magnitude, from ~106 to ~108 CFU/mL). Ag NPs showed equal influence on both tested microorganisms, with dextrane-coated Ag NPs were more effective. In the experiment with organic mung beans, the same colloid solutions of Ag NPs and TiO2 NPs had a stimulating effect on the germination and root length of this plant, also. The number of germinated seeds was the same as in the control, while the average root length was higher in the samples than in the control. The average root length was 1.47 cm in the control. Seeds treated with dextrane-coated Ag NPs had an average root length even 82 % higher compared to the control. The stimulatory effect was also observed in the case of seeds treated with Ag NPs synthesized by chemical reduction and TiO2 NPs, where the average root lengths were higher for 40% and 76 %, respectively than in the control. Germination index values, which are higher than 100%, suggesting that tested nanoparticles can be used as fertilizers in agriculture. Moreover, the obtained results show that silver nanoparticles and titanium dioxide nanoparticles have a stimulatory effect on the soil bacteria and organic mung bean, which prove their safety for this kind of application.",
journal = "Proceedings,VII International Congress “ Engineering, Environment and Materials in Process Industry",
title = "Investigation of the influence of different nanoparticles on the growth of soil microorganisms and organic mung bean",
pages = "460-451",
url = "https://hdl.handle.net/21.15107/rcub_technorep_5927"
}

Ilić, N., Lazić, V., Radovanović, N., Mihajlovski, K., Davidović, S.,& Miljković, M.. (2021). Investigation of the influence of different nanoparticles on the growth of soil microorganisms and organic mung bean. in Proceedings,VII International Congress “ Engineering, Environment and Materials in Process Industry, 451-460.
https://hdl.handle.net/21.15107/rcub_technorep_5927

Ilić N, Lazić V, Radovanović N, Mihajlovski K, Davidović S, Miljković M. Investigation of the influence of different nanoparticles on the growth of soil microorganisms and organic mung bean. in Proceedings,VII International Congress “ Engineering, Environment and Materials in Process Industry. 2021;:451-460.
https://hdl.handle.net/21.15107/rcub_technorep_5927 .

Ilić, Nevena, Lazić, Vesna, Radovanović, Neda, Mihajlovski, Katarina, Davidović, Slađana, Miljković, Miona, "Investigation of the influence of different nanoparticles on the growth of soil microorganisms and organic mung bean" in Proceedings,VII International Congress “ Engineering, Environment and Materials in Process Industry (2021):451-460,
https://hdl.handle.net/21.15107/rcub_technorep_5927 .

TY  - JOUR
AU  - Miljković, Miona
AU  - Davidović, Slađana
AU  - Đukić-Vuković, Aleksandra
AU  - Ilic, Mila, V
AU  - Simović, Milica
AU  - Rajilić-Stojanović, Mirjana
AU  - Dimitrijević-Branković, Suzana
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4791
AB  - Dextransucrase (DS) is a glucosyltransferase (E. C. 2.4.1.5) that catalyzes the transfer of glucosyl residues from sucrose to dextran polymer and liberates fructose. This enzyme isassociated with a wide application range of dextran and oligosaccharides. DS production by Leuconostoc mesenteroidesT3 was optimized using a Central Composite Design under the Response Surface Methodology. Three variables were chosen for optimization: distillery stillage, sucrose and manganese concentration. The results showed that sucrose and manganese concentrations had a positive linear effect on DS production while all variable interactions (stillage-manganese, stillage-sucrose, and sucrose-manganese) had significant influences on the DS production. The maximal DS yield of 3.391 +/- 0.131 U cm-3, was obtained in the medium with 64.33 % distillery stillage concentration, 5.30% sucrose concentration and 0.022 % manganese concentration. Our study revealed the potential of distillery stillage combined with sugar beet molasses, supplemented with sucrose and manganese to be employed as a valuable medium growth for lactic acid bacteria and production of DS. Also, taking into consideration the origin of the substrates, utilization of industrial by-products in this way has a great environmental relevance and is in accordance with circular economy.
T2  - Hemijska industrija
T1  - Utilization of agro-industrial by-products as substrates for dextransucrase production by Leuconostoc mesenteroides T3: process optimization using response surface methodology
EP  - 146
IS  - 3
SP  - 135
VL  - 75
DO  - 10.2298/HEMIND200710015M
ER  - 

@article{
author = "Miljković, Miona and Davidović, Slađana and Đukić-Vuković, Aleksandra and Ilic, Mila, V and Simović, Milica and Rajilić-Stojanović, Mirjana and Dimitrijević-Branković, Suzana",
year = "2021",
abstract = "Dextransucrase (DS) is a glucosyltransferase (E. C. 2.4.1.5) that catalyzes the transfer of glucosyl residues from sucrose to dextran polymer and liberates fructose. This enzyme isassociated with a wide application range of dextran and oligosaccharides. DS production by Leuconostoc mesenteroidesT3 was optimized using a Central Composite Design under the Response Surface Methodology. Three variables were chosen for optimization: distillery stillage, sucrose and manganese concentration. The results showed that sucrose and manganese concentrations had a positive linear effect on DS production while all variable interactions (stillage-manganese, stillage-sucrose, and sucrose-manganese) had significant influences on the DS production. The maximal DS yield of 3.391 +/- 0.131 U cm-3, was obtained in the medium with 64.33 % distillery stillage concentration, 5.30% sucrose concentration and 0.022 % manganese concentration. Our study revealed the potential of distillery stillage combined with sugar beet molasses, supplemented with sucrose and manganese to be employed as a valuable medium growth for lactic acid bacteria and production of DS. Also, taking into consideration the origin of the substrates, utilization of industrial by-products in this way has a great environmental relevance and is in accordance with circular economy.",
journal = "Hemijska industrija",
title = "Utilization of agro-industrial by-products as substrates for dextransucrase production by Leuconostoc mesenteroides T3: process optimization using response surface methodology",
pages = "146-135",
number = "3",
volume = "75",
doi = "10.2298/HEMIND200710015M"
}

Miljković, M., Davidović, S., Đukić-Vuković, A., Ilic, M. V., Simović, M., Rajilić-Stojanović, M.,& Dimitrijević-Branković, S.. (2021). Utilization of agro-industrial by-products as substrates for dextransucrase production by Leuconostoc mesenteroides T3: process optimization using response surface methodology. in Hemijska industrija, 75(3), 135-146.
https://doi.org/10.2298/HEMIND200710015M

Miljković M, Davidović S, Đukić-Vuković A, Ilic MV, Simović M, Rajilić-Stojanović M, Dimitrijević-Branković S. Utilization of agro-industrial by-products as substrates for dextransucrase production by Leuconostoc mesenteroides T3: process optimization using response surface methodology. in Hemijska industrija. 2021;75(3):135-146.
doi:10.2298/HEMIND200710015M .

Miljković, Miona, Davidović, Slađana, Đukić-Vuković, Aleksandra, Ilic, Mila, V, Simović, Milica, Rajilić-Stojanović, Mirjana, Dimitrijević-Branković, Suzana, "Utilization of agro-industrial by-products as substrates for dextransucrase production by Leuconostoc mesenteroides T3: process optimization using response surface methodology" in Hemijska industrija, 75, no. 3 (2021):135-146,
https://doi.org/10.2298/HEMIND200710015M . .

TY  - JOUR
AU  - Davidović, Slađana
AU  - Miljković, Miona
AU  - Gordic, Milan
AU  - Cabrera-Barjas, Gustavo
AU  - Nesic, Aleksandra
AU  - Dimitrijević-Branković, Suzana
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4942
AB  - The development of edible films and coatings in the food packaging industry presents one of the modern strategies for protecting food products and ensuring their freshness and quality during their shelf lives. The application of microbial polysaccharides to the development of food package materials, as an alternative option to the commonly used plastic materials, is both economic and environmentally favorable. New edible films were developed using dextran from lactic acid bacterium Leuconostoc mesenteroides T3, and additionally plasticized by different concentrations of polyglycerol. The best tensile strength of the films was obtained using a formulation that contained 10 wt% of polyglycerol, which corresponded to a value of 4.6 MPa. The most flexible formulation, with elongation at break of 602%, was obtained with 30 wt% of polyglycerol. Water vapor permeability values of the films synthesized in this study were in the range of (3.45-8.81) * 10(-12) g/m s Pa. Such low values indicated that they could be efficient in preventing fruit from drying out during storage. Thus, the film formulations were used to coat blueberries in order to assess their quality during a storage time of 21 days at 8 degrees C. The results showed that dextran/polyglycerol films could be efficient in extending the shelf life of blueberries, which was evidenced by lower weight loss and total sugar solids values, as well as a delay in titratable acidity, in comparison to the uncoated blueberries.
T2  - Polymers
T1  - Dextran-Based Edible Coatings to Prolong the Shelf Life of Blueberries
IS  - 23
VL  - 13
DO  - 10.3390/polym13234252
ER  - 

@article{
author = "Davidović, Slađana and Miljković, Miona and Gordic, Milan and Cabrera-Barjas, Gustavo and Nesic, Aleksandra and Dimitrijević-Branković, Suzana",
year = "2021",
abstract = "The development of edible films and coatings in the food packaging industry presents one of the modern strategies for protecting food products and ensuring their freshness and quality during their shelf lives. The application of microbial polysaccharides to the development of food package materials, as an alternative option to the commonly used plastic materials, is both economic and environmentally favorable. New edible films were developed using dextran from lactic acid bacterium Leuconostoc mesenteroides T3, and additionally plasticized by different concentrations of polyglycerol. The best tensile strength of the films was obtained using a formulation that contained 10 wt% of polyglycerol, which corresponded to a value of 4.6 MPa. The most flexible formulation, with elongation at break of 602%, was obtained with 30 wt% of polyglycerol. Water vapor permeability values of the films synthesized in this study were in the range of (3.45-8.81) * 10(-12) g/m s Pa. Such low values indicated that they could be efficient in preventing fruit from drying out during storage. Thus, the film formulations were used to coat blueberries in order to assess their quality during a storage time of 21 days at 8 degrees C. The results showed that dextran/polyglycerol films could be efficient in extending the shelf life of blueberries, which was evidenced by lower weight loss and total sugar solids values, as well as a delay in titratable acidity, in comparison to the uncoated blueberries.",
journal = "Polymers",
title = "Dextran-Based Edible Coatings to Prolong the Shelf Life of Blueberries",
number = "23",
volume = "13",
doi = "10.3390/polym13234252"
}

Davidović, S., Miljković, M., Gordic, M., Cabrera-Barjas, G., Nesic, A.,& Dimitrijević-Branković, S.. (2021). Dextran-Based Edible Coatings to Prolong the Shelf Life of Blueberries. in Polymers, 13(23).
https://doi.org/10.3390/polym13234252

Davidović S, Miljković M, Gordic M, Cabrera-Barjas G, Nesic A, Dimitrijević-Branković S. Dextran-Based Edible Coatings to Prolong the Shelf Life of Blueberries. in Polymers. 2021;13(23).
doi:10.3390/polym13234252 .

Davidović, Slađana, Miljković, Miona, Gordic, Milan, Cabrera-Barjas, Gustavo, Nesic, Aleksandra, Dimitrijević-Branković, Suzana, "Dextran-Based Edible Coatings to Prolong the Shelf Life of Blueberries" in Polymers, 13, no. 23 (2021),
https://doi.org/10.3390/polym13234252 . .

TY  - JOUR
AU  - Miljković, Miona
AU  - Lazić, Vesna
AU  - Davidović, Slađana
AU  - Milivojević, Ana
AU  - Papan, Jelena
AU  - Fernandes, Margarida M.
AU  - Lanceros-Mendez, Senentxu
AU  - Ahrenkiel, Scott Phillip
AU  - Nedeljković, Jovan
PY  - 2020
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4351
AB  - The aim of this study was the development of a non-toxic, biosynthetic antimicrobial agent which selectively acts on only one type of microorganism, and preserves the microbiota. Antimicrobial performance of biosynthesized silver nanoparticles (Ag NPs) by horsetail (Equisetum arvense L.) extract was examined against Gram-negative bacteria Escherichia coli and Gram-positive bacteria Staphylococcus aureus, as well as yeasts Candida albicans and Saccharomyces boulardii. Also, the cytotoxicity of Ag NPs was examined toward pre-osteoblast cells. The synthetic conditions-concentration of extract, temperature, and pH-were optimized to prepare silver colloids with different particle size distributions and long-term stability. The obtained samples were characterized using transmission electron microscopy, X-ray diffraction analysis, and absorption spectroscopy. The smaller-sized Ag NPs ( 10-20 nm), prepared at a lower temperature (20 degrees C), showed better antimicrobial performance against E. coli compared to larger ones ( 40-60 nm), prepared at high temperature (100 degrees C). On the other hand, both samples did not display any toxic action against bacteria S. aureus, or yeasts C. albicans and S. boulardii. Non-cytotoxic behavior of Ag NPs toward pre-osteoblast cells was observed for the concentrations of silver  lt = 2.25 and  lt = 4.5 mg L-1 for 10-20 and 40-60 nm-sized Ag NPs, respectively. Biosynthesized Ag NPs by horsetail extract display selective toxic action against E. coli at the ecologically acceptable concentration level.
PB  - Springer, Dordrecht
T2  - Journal of Inorganic and Organometallic Polymers and Materials
T1  - Selective Antimicrobial Performance of Biosynthesized Silver Nanoparticles by Horsetail Extract Against E. coli
EP  - 2607
IS  - 7
SP  - 2598
VL  - 30
DO  - 10.1007/s10904-019-01402-x
ER  - 

@article{
author = "Miljković, Miona and Lazić, Vesna and Davidović, Slađana and Milivojević, Ana and Papan, Jelena and Fernandes, Margarida M. and Lanceros-Mendez, Senentxu and Ahrenkiel, Scott Phillip and Nedeljković, Jovan",
year = "2020",
abstract = "The aim of this study was the development of a non-toxic, biosynthetic antimicrobial agent which selectively acts on only one type of microorganism, and preserves the microbiota. Antimicrobial performance of biosynthesized silver nanoparticles (Ag NPs) by horsetail (Equisetum arvense L.) extract was examined against Gram-negative bacteria Escherichia coli and Gram-positive bacteria Staphylococcus aureus, as well as yeasts Candida albicans and Saccharomyces boulardii. Also, the cytotoxicity of Ag NPs was examined toward pre-osteoblast cells. The synthetic conditions-concentration of extract, temperature, and pH-were optimized to prepare silver colloids with different particle size distributions and long-term stability. The obtained samples were characterized using transmission electron microscopy, X-ray diffraction analysis, and absorption spectroscopy. The smaller-sized Ag NPs ( 10-20 nm), prepared at a lower temperature (20 degrees C), showed better antimicrobial performance against E. coli compared to larger ones ( 40-60 nm), prepared at high temperature (100 degrees C). On the other hand, both samples did not display any toxic action against bacteria S. aureus, or yeasts C. albicans and S. boulardii. Non-cytotoxic behavior of Ag NPs toward pre-osteoblast cells was observed for the concentrations of silver  lt = 2.25 and  lt = 4.5 mg L-1 for 10-20 and 40-60 nm-sized Ag NPs, respectively. Biosynthesized Ag NPs by horsetail extract display selective toxic action against E. coli at the ecologically acceptable concentration level.",
publisher = "Springer, Dordrecht",
journal = "Journal of Inorganic and Organometallic Polymers and Materials",
title = "Selective Antimicrobial Performance of Biosynthesized Silver Nanoparticles by Horsetail Extract Against E. coli",
pages = "2607-2598",
number = "7",
volume = "30",
doi = "10.1007/s10904-019-01402-x"
}

Miljković, M., Lazić, V., Davidović, S., Milivojević, A., Papan, J., Fernandes, M. M., Lanceros-Mendez, S., Ahrenkiel, S. P.,& Nedeljković, J.. (2020). Selective Antimicrobial Performance of Biosynthesized Silver Nanoparticles by Horsetail Extract Against E. coli. in Journal of Inorganic and Organometallic Polymers and Materials
Springer, Dordrecht., 30(7), 2598-2607.
https://doi.org/10.1007/s10904-019-01402-x

Miljković M, Lazić V, Davidović S, Milivojević A, Papan J, Fernandes MM, Lanceros-Mendez S, Ahrenkiel SP, Nedeljković J. Selective Antimicrobial Performance of Biosynthesized Silver Nanoparticles by Horsetail Extract Against E. coli. in Journal of Inorganic and Organometallic Polymers and Materials. 2020;30(7):2598-2607.
doi:10.1007/s10904-019-01402-x .

Miljković, Miona, Lazić, Vesna, Davidović, Slađana, Milivojević, Ana, Papan, Jelena, Fernandes, Margarida M., Lanceros-Mendez, Senentxu, Ahrenkiel, Scott Phillip, Nedeljković, Jovan, "Selective Antimicrobial Performance of Biosynthesized Silver Nanoparticles by Horsetail Extract Against E. coli" in Journal of Inorganic and Organometallic Polymers and Materials, 30, no. 7 (2020):2598-2607,
https://doi.org/10.1007/s10904-019-01402-x . .

TY  - JOUR
AU  - Davidović, Slađana
AU  - Lazić, Vesna M.
AU  - Miljković, Miona
AU  - Gordić, Milan V.
AU  - Sekulić, Milica
AU  - Marinović-Cincović, Milena
AU  - Ratnayake, Ishara S.
AU  - Ahrenkiel, Scott Phillip
AU  - Nedeljković, Jovan
PY  - 2019
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4081
AB  - The antibacterial ability of in situ prepared nanometer-sized silver particles, immobilized in agar-agar films, was studied as a function of the concentration of co-dopant, magnesium ions. Content of inorganic components in hybrid films was determined using inductively coupled plasma optic emission spectroscopy, and found to be low (  lt  2 wt.-%). Morphology of prepared hybrid films, studied by transmission electron microscopy, revealed the presence of non-agglomerated and randomly distributed 10-20 nm silver nanoparticles (Ag NPs) within the agar-agar matrices. Fourier-transform infrared spectroscopy indicated the distinct chemical interaction between Ag NPs and polymer chains. Thermogravimetric analysis, as well as the determination of tensile strength, Young's modulus, and elongation at break showed improvement of thermal stability and mechanical properties of agar-agar matrices upon the incorporation of Ag NPs due to high compatibility between the hydrophilic organic component and inorganic components. The complete microbial reduction of Gram-positive bacteria Staphylococcus aureus was observed for all agar-silver films, while satisfactory results were observed for Gram-negative bacteria Pseudomonas aeruginosa (  gt = 99.6%). The release of Ag+ ions is suppressed by the increase of the concentration of Mg2+ ions and it was found to be significantly smaller (  lt = 0.24 ppm) than the harmful ecological level (1 ppm).
PB  - Elsevier Sci Ltd, Oxford
T2  - Carbohydrate Polymers
T1  - Antibacterial ability of immobilized silver nanoparticles in agar-agar films co-doped with magnesium ions
VL  - 224
DO  - 10.1016/j.carbpol.2019.115187
ER  - 

@article{
author = "Davidović, Slađana and Lazić, Vesna M. and Miljković, Miona and Gordić, Milan V. and Sekulić, Milica and Marinović-Cincović, Milena and Ratnayake, Ishara S. and Ahrenkiel, Scott Phillip and Nedeljković, Jovan",
year = "2019",
abstract = "The antibacterial ability of in situ prepared nanometer-sized silver particles, immobilized in agar-agar films, was studied as a function of the concentration of co-dopant, magnesium ions. Content of inorganic components in hybrid films was determined using inductively coupled plasma optic emission spectroscopy, and found to be low (  lt  2 wt.-%). Morphology of prepared hybrid films, studied by transmission electron microscopy, revealed the presence of non-agglomerated and randomly distributed 10-20 nm silver nanoparticles (Ag NPs) within the agar-agar matrices. Fourier-transform infrared spectroscopy indicated the distinct chemical interaction between Ag NPs and polymer chains. Thermogravimetric analysis, as well as the determination of tensile strength, Young's modulus, and elongation at break showed improvement of thermal stability and mechanical properties of agar-agar matrices upon the incorporation of Ag NPs due to high compatibility between the hydrophilic organic component and inorganic components. The complete microbial reduction of Gram-positive bacteria Staphylococcus aureus was observed for all agar-silver films, while satisfactory results were observed for Gram-negative bacteria Pseudomonas aeruginosa (  gt = 99.6%). The release of Ag+ ions is suppressed by the increase of the concentration of Mg2+ ions and it was found to be significantly smaller (  lt = 0.24 ppm) than the harmful ecological level (1 ppm).",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Carbohydrate Polymers",
title = "Antibacterial ability of immobilized silver nanoparticles in agar-agar films co-doped with magnesium ions",
volume = "224",
doi = "10.1016/j.carbpol.2019.115187"
}

Davidović, S., Lazić, V. M., Miljković, M., Gordić, M. V., Sekulić, M., Marinović-Cincović, M., Ratnayake, I. S., Ahrenkiel, S. P.,& Nedeljković, J.. (2019). Antibacterial ability of immobilized silver nanoparticles in agar-agar films co-doped with magnesium ions. in Carbohydrate Polymers
Elsevier Sci Ltd, Oxford., 224.
https://doi.org/10.1016/j.carbpol.2019.115187

Davidović S, Lazić VM, Miljković M, Gordić MV, Sekulić M, Marinović-Cincović M, Ratnayake IS, Ahrenkiel SP, Nedeljković J. Antibacterial ability of immobilized silver nanoparticles in agar-agar films co-doped with magnesium ions. in Carbohydrate Polymers. 2019;224.
doi:10.1016/j.carbpol.2019.115187 .

Davidović, Slađana, Lazić, Vesna M., Miljković, Miona, Gordić, Milan V., Sekulić, Milica, Marinović-Cincović, Milena, Ratnayake, Ishara S., Ahrenkiel, Scott Phillip, Nedeljković, Jovan, "Antibacterial ability of immobilized silver nanoparticles in agar-agar films co-doped with magnesium ions" in Carbohydrate Polymers, 224 (2019),
https://doi.org/10.1016/j.carbpol.2019.115187 . .

TY  - CONF
AU  - Radovanović, Neda
AU  - Miljković, Miona
AU  - Davidović, Slađana
AU  - Malagurski, Ivana
AU  - Gordić, Milan
AU  - Nešić, Aleksandra
AU  - Dimitrijević-Branković, Suzana
PY  - 2018
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6286
C3  - Polychar 26 World Forum on Advanced Materials
T1  - ACTIVE AGAR MINERALIZED COMPOSITE FILMS INTENDED FOR FOOD PACKAGING
SP  - 94
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6286
ER  - 

@conference{
author = "Radovanović, Neda and Miljković, Miona and Davidović, Slađana and Malagurski, Ivana and Gordić, Milan and Nešić, Aleksandra and Dimitrijević-Branković, Suzana",
year = "2018",
journal = "Polychar 26 World Forum on Advanced Materials",
title = "ACTIVE AGAR MINERALIZED COMPOSITE FILMS INTENDED FOR FOOD PACKAGING",
pages = "94",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6286"
}

Radovanović, N., Miljković, M., Davidović, S., Malagurski, I., Gordić, M., Nešić, A.,& Dimitrijević-Branković, S.. (2018). ACTIVE AGAR MINERALIZED COMPOSITE FILMS INTENDED FOR FOOD PACKAGING. in Polychar 26 World Forum on Advanced Materials, 94.
https://hdl.handle.net/21.15107/rcub_technorep_6286

Radovanović N, Miljković M, Davidović S, Malagurski I, Gordić M, Nešić A, Dimitrijević-Branković S. ACTIVE AGAR MINERALIZED COMPOSITE FILMS INTENDED FOR FOOD PACKAGING. in Polychar 26 World Forum on Advanced Materials. 2018;:94.
https://hdl.handle.net/21.15107/rcub_technorep_6286 .

Radovanović, Neda, Miljković, Miona, Davidović, Slađana, Malagurski, Ivana, Gordić, Milan, Nešić, Aleksandra, Dimitrijević-Branković, Suzana, "ACTIVE AGAR MINERALIZED COMPOSITE FILMS INTENDED FOR FOOD PACKAGING" in Polychar 26 World Forum on Advanced Materials (2018):94,
https://hdl.handle.net/21.15107/rcub_technorep_6286 .

TY  - JOUR
AU  - Miljković, Miona
AU  - Lazić, Vesna M.
AU  - Banjanac, Katarina
AU  - Davidović, Slađana
AU  - Bezbradica, Dejan
AU  - Marinković, Aleksandar
AU  - Sredojević, Dušan
AU  - Nedeljković, Jovan
AU  - Dimitrijević-Branković, Suzana
PY  - 2018
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4014
AB  - The TiO2 based hybrid supports with different functional groups (amino, glutaraldehyde or epoxy) were prepared and their influence on immobilization of dextransucrase (DS) was studied. Novel synthetic route for surface modification of TiO2 with amino and glutaraldehyde groups was developed taking advantage of charge transfer complex (CTC) formation between surface Ti atoms and salicylate-type of ligand (5 aminosalicylic acid (5-ASA)). The proposed coordination of 5-ASA to the surface of TiO2 powder and optical properties of CTC was presented. The pristine TiO2 and amino functionalized TiO2 have higher sorption capacity for DS (12.6 and 12.0 mg g(-1), respectively) compared to glutaraldehyde and epoxy activated supports (9.6 and 9.8 mg g(-1) respectively). However, immobilized enzyme to either glutaraldehyde or epoxy functionalized TiO2 have almost two times higher expressed activities compared to pristine TiO2 support (258, 235 and 142 IU g(-1), respectively). Thermal stability of enzyme immobilized on glutaraldehyde and epoxy functionalized supports was studied at 40 degrees C, as well as operational stability under long-run working conditions in repeated cycles. After five cycles, DS imobilized on glutaraldehyde activated support retained almost 70% of its initial expressed activity, while, after five cycles, performance of DS immobilized on epoxy activated support was significantly lower (15%).
PB  - Elsevier Science Bv, Amsterdam
T2  - International Journal of Biological Macromolecules
T1  - Immobilization of dextransucrase on functionalized TiO2 supports
EP  - 1223
SP  - 1216
VL  - 114
DO  - 10.1016/j.ijbiomac.2018.04.027
ER  - 

@article{
author = "Miljković, Miona and Lazić, Vesna M. and Banjanac, Katarina and Davidović, Slađana and Bezbradica, Dejan and Marinković, Aleksandar and Sredojević, Dušan and Nedeljković, Jovan and Dimitrijević-Branković, Suzana",
year = "2018",
abstract = "The TiO2 based hybrid supports with different functional groups (amino, glutaraldehyde or epoxy) were prepared and their influence on immobilization of dextransucrase (DS) was studied. Novel synthetic route for surface modification of TiO2 with amino and glutaraldehyde groups was developed taking advantage of charge transfer complex (CTC) formation between surface Ti atoms and salicylate-type of ligand (5 aminosalicylic acid (5-ASA)). The proposed coordination of 5-ASA to the surface of TiO2 powder and optical properties of CTC was presented. The pristine TiO2 and amino functionalized TiO2 have higher sorption capacity for DS (12.6 and 12.0 mg g(-1), respectively) compared to glutaraldehyde and epoxy activated supports (9.6 and 9.8 mg g(-1) respectively). However, immobilized enzyme to either glutaraldehyde or epoxy functionalized TiO2 have almost two times higher expressed activities compared to pristine TiO2 support (258, 235 and 142 IU g(-1), respectively). Thermal stability of enzyme immobilized on glutaraldehyde and epoxy functionalized supports was studied at 40 degrees C, as well as operational stability under long-run working conditions in repeated cycles. After five cycles, DS imobilized on glutaraldehyde activated support retained almost 70% of its initial expressed activity, while, after five cycles, performance of DS immobilized on epoxy activated support was significantly lower (15%).",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "International Journal of Biological Macromolecules",
title = "Immobilization of dextransucrase on functionalized TiO2 supports",
pages = "1223-1216",
volume = "114",
doi = "10.1016/j.ijbiomac.2018.04.027"
}

Miljković, M., Lazić, V. M., Banjanac, K., Davidović, S., Bezbradica, D., Marinković, A., Sredojević, D., Nedeljković, J.,& Dimitrijević-Branković, S.. (2018). Immobilization of dextransucrase on functionalized TiO2 supports. in International Journal of Biological Macromolecules
Elsevier Science Bv, Amsterdam., 114, 1216-1223.
https://doi.org/10.1016/j.ijbiomac.2018.04.027

Miljković M, Lazić VM, Banjanac K, Davidović S, Bezbradica D, Marinković A, Sredojević D, Nedeljković J, Dimitrijević-Branković S. Immobilization of dextransucrase on functionalized TiO2 supports. in International Journal of Biological Macromolecules. 2018;114:1216-1223.
doi:10.1016/j.ijbiomac.2018.04.027 .

Miljković, Miona, Lazić, Vesna M., Banjanac, Katarina, Davidović, Slađana, Bezbradica, Dejan, Marinković, Aleksandar, Sredojević, Dušan, Nedeljković, Jovan, Dimitrijević-Branković, Suzana, "Immobilization of dextransucrase on functionalized TiO2 supports" in International Journal of Biological Macromolecules, 114 (2018):1216-1223,
https://doi.org/10.1016/j.ijbiomac.2018.04.027 . .

TY  - JOUR
AU  - Davidović, Slađana
AU  - Miljković, Miona
AU  - Tomić, Miloš
AU  - Gordić, Milan V.
AU  - Nešić, Aleksandra
AU  - Dimitrijević, Suzana
PY  - 2018
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4017
AB  - The aim of this study was to develop dextran-based edible films plasticized by sorbitol. In order to optimise the film-forming formulation, response surface methodology was used. The influence of dextran and sorbitol concentration on the mechanical and water vapour barrier properties of obtained films was investigated. The results showed that both parameters exhibited significant effect on the water vapour permeability of a film. Both dextran and sorbitol concentration had significant influence on tensile strength and elongation at break, whereas only sorbitol concentration had significant effect on Young's modulus. After optimisation by desirability approach, it was found that a film made of 3.40 wt% of dextran and 20.43 wt% of sorbitol showed the lowest water vapour permeability and the highest tensile strength and elasticity.
PB  - Elsevier Sci Ltd, Oxford
T2  - Carbohydrate Polymers
T1  - Response surface methodology for optimisation of edible coatings based on dextran from Leuconostoc mesenteroides T3
EP  - 213
SP  - 207
VL  - 184
DO  - 10.1016/j.carbpol.2017.12.061
ER  - 

@article{
author = "Davidović, Slađana and Miljković, Miona and Tomić, Miloš and Gordić, Milan V. and Nešić, Aleksandra and Dimitrijević, Suzana",
year = "2018",
abstract = "The aim of this study was to develop dextran-based edible films plasticized by sorbitol. In order to optimise the film-forming formulation, response surface methodology was used. The influence of dextran and sorbitol concentration on the mechanical and water vapour barrier properties of obtained films was investigated. The results showed that both parameters exhibited significant effect on the water vapour permeability of a film. Both dextran and sorbitol concentration had significant influence on tensile strength and elongation at break, whereas only sorbitol concentration had significant effect on Young's modulus. After optimisation by desirability approach, it was found that a film made of 3.40 wt% of dextran and 20.43 wt% of sorbitol showed the lowest water vapour permeability and the highest tensile strength and elasticity.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Carbohydrate Polymers",
title = "Response surface methodology for optimisation of edible coatings based on dextran from Leuconostoc mesenteroides T3",
pages = "213-207",
volume = "184",
doi = "10.1016/j.carbpol.2017.12.061"
}

Davidović, S., Miljković, M., Tomić, M., Gordić, M. V., Nešić, A.,& Dimitrijević, S.. (2018). Response surface methodology for optimisation of edible coatings based on dextran from Leuconostoc mesenteroides T3. in Carbohydrate Polymers
Elsevier Sci Ltd, Oxford., 184, 207-213.
https://doi.org/10.1016/j.carbpol.2017.12.061

Davidović S, Miljković M, Tomić M, Gordić MV, Nešić A, Dimitrijević S. Response surface methodology for optimisation of edible coatings based on dextran from Leuconostoc mesenteroides T3. in Carbohydrate Polymers. 2018;184:207-213.
doi:10.1016/j.carbpol.2017.12.061 .

Davidović, Slađana, Miljković, Miona, Tomić, Miloš, Gordić, Milan V., Nešić, Aleksandra, Dimitrijević, Suzana, "Response surface methodology for optimisation of edible coatings based on dextran from Leuconostoc mesenteroides T3" in Carbohydrate Polymers, 184 (2018):207-213,
https://doi.org/10.1016/j.carbpol.2017.12.061 . .

TY  - CONF
AU  - Davidović, Slađana
AU  - Miljković, Miona
AU  - Radovanović, Neda
AU  - Gordić, Milan
AU  - Nešić, Aleksandra
AU  - Dimitrijević, Suzana
PY  - 2018
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6204
AB  - Current trends in food biotechnology are focused on replacing synthetic polymers used for food packages by the natural ones. Biopolymers are made by sustainable processes in a cheap way. Moreover, they are nontoxic and can serve as a good alternative to traditional (petroleum) food packaging, due to great film-formation properties in a form of edible films and coatings for food products. Various polysaccharides, proteins, and lipids have been intensively investigated as edible materials that could improve the food quality, freshness, and provide food safety. However, natural polymers usually lack of antioxidative and/or antimicrobial properties. Therefore, many synthetic and natural additives can be incorporated into edible films. Among them, extracts of medicinal plants are giving more attention due to their safeness and proved health benefits.
The aim of this study was to obtain edible films based on dextran, with antioxidative and antimicrobial properties. Dextran obtained in our lab was used for preparing edible film with sorbitol as a plasticizer, while pulverized waste biomass obtained from the processing of medicinal herb yarrow (Achillea millefolium) was used as a source of polyphenols. It has previously been shown1 that dextran based films had excellent mechanical and water vapor barrier properties. In this work, spray dried ethanol extract of Achillea millefolium dust (AME) was incorporated into dextran films in concentrations 2, 3, and 4% (w/v). The composite films were analyzed regarding antioxidative, antimicrobial, and mechanical properties. All films showed high antioxidative activity (90% reduction of DPPH radical). However, to obtain high antimicrobial activity (higher than 50%), 4% of AME was required. Composite films showed antimicrobial activity against three Gram-positive bacteria (Staphylococcus aureus, Listeria monocytogenes and Enterococcus faecalis and one Gram-negative bacterium (Pseudomonas aeruginosa). Incorporation of AME into dextran film showed positive effect on tensile strength and negative effect on elongation. Composite film with the best mechanical properties (tensile strength 3.5 MPa and elongation at break 37%) was the film containing 4% AME.
C3  - Polychar 26 World Forum on Advanced Materials
T1  - ANTIOXIDANT AND ANTIMICROBIAL EDIBLE FILMS BASED ON DEXTRAN CONTAINING POLYPHENOLS FROM YARROW EXTRACT
SP  - 72
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6204
ER  - 

@conference{
author = "Davidović, Slađana and Miljković, Miona and Radovanović, Neda and Gordić, Milan and Nešić, Aleksandra and Dimitrijević, Suzana",
year = "2018",
abstract = "Current trends in food biotechnology are focused on replacing synthetic polymers used for food packages by the natural ones. Biopolymers are made by sustainable processes in a cheap way. Moreover, they are nontoxic and can serve as a good alternative to traditional (petroleum) food packaging, due to great film-formation properties in a form of edible films and coatings for food products. Various polysaccharides, proteins, and lipids have been intensively investigated as edible materials that could improve the food quality, freshness, and provide food safety. However, natural polymers usually lack of antioxidative and/or antimicrobial properties. Therefore, many synthetic and natural additives can be incorporated into edible films. Among them, extracts of medicinal plants are giving more attention due to their safeness and proved health benefits.
The aim of this study was to obtain edible films based on dextran, with antioxidative and antimicrobial properties. Dextran obtained in our lab was used for preparing edible film with sorbitol as a plasticizer, while pulverized waste biomass obtained from the processing of medicinal herb yarrow (Achillea millefolium) was used as a source of polyphenols. It has previously been shown1 that dextran based films had excellent mechanical and water vapor barrier properties. In this work, spray dried ethanol extract of Achillea millefolium dust (AME) was incorporated into dextran films in concentrations 2, 3, and 4% (w/v). The composite films were analyzed regarding antioxidative, antimicrobial, and mechanical properties. All films showed high antioxidative activity (90% reduction of DPPH radical). However, to obtain high antimicrobial activity (higher than 50%), 4% of AME was required. Composite films showed antimicrobial activity against three Gram-positive bacteria (Staphylococcus aureus, Listeria monocytogenes and Enterococcus faecalis and one Gram-negative bacterium (Pseudomonas aeruginosa). Incorporation of AME into dextran film showed positive effect on tensile strength and negative effect on elongation. Composite film with the best mechanical properties (tensile strength 3.5 MPa and elongation at break 37%) was the film containing 4% AME.",
journal = "Polychar 26 World Forum on Advanced Materials",
title = "ANTIOXIDANT AND ANTIMICROBIAL EDIBLE FILMS BASED ON DEXTRAN CONTAINING POLYPHENOLS FROM YARROW EXTRACT",
pages = "72",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6204"
}

Davidović, S., Miljković, M., Radovanović, N., Gordić, M., Nešić, A.,& Dimitrijević, S.. (2018). ANTIOXIDANT AND ANTIMICROBIAL EDIBLE FILMS BASED ON DEXTRAN CONTAINING POLYPHENOLS FROM YARROW EXTRACT. in Polychar 26 World Forum on Advanced Materials, 72.
https://hdl.handle.net/21.15107/rcub_technorep_6204

Davidović S, Miljković M, Radovanović N, Gordić M, Nešić A, Dimitrijević S. ANTIOXIDANT AND ANTIMICROBIAL EDIBLE FILMS BASED ON DEXTRAN CONTAINING POLYPHENOLS FROM YARROW EXTRACT. in Polychar 26 World Forum on Advanced Materials. 2018;:72.
https://hdl.handle.net/21.15107/rcub_technorep_6204 .

Davidović, Slađana, Miljković, Miona, Radovanović, Neda, Gordić, Milan, Nešić, Aleksandra, Dimitrijević, Suzana, "ANTIOXIDANT AND ANTIMICROBIAL EDIBLE FILMS BASED ON DEXTRAN CONTAINING POLYPHENOLS FROM YARROW EXTRACT" in Polychar 26 World Forum on Advanced Materials (2018):72,
https://hdl.handle.net/21.15107/rcub_technorep_6204 .

TY  - CONF
AU  - Miljković, Miona
AU  - Davidović, Slađana
AU  - Radovanović, Neda
AU  - Gordić, Milan
AU  - Carević, Milica
AU  - Nešić, Aleksandra
AU  - Dimitrijević, Suzana
PY  - 2018
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6239
AB  - Dextransucrase (DS), the extracellular enzyme is of immense industrial importance, due to ability to produce dextran and oligosaccharides (OS). Worldwide interest in OS has been increasing, since they have been accorded the prebiotic status. However, the industrial application of DS for OS synthesis is limited, due to low yield of enzyme production and its low catalytic activity. Hence, there is a great interest for development of new technologies that can provide improved performance of biocatalyst. Enzyme immobilization technology is considered to be a crucial step for cheaper and more efficient usage of DS. Entrapment is one of the widely investigated immobilization methods, where enzymes are enclosed or confined within the polymer matrix without altering their native structure, developing bioreactors for commercial applications. Different matrices such as polyacryl-amide gel, alginate beads and agar–agar have been used for the entrapment of different enzymes and among them agar–agar is a biocompatible, non-toxic and strong solidifying agent for immobilization of various enzymes.
In this work, the entrapment of DS was initiated by different quantity (1:9, 1:4 and 1:1) of dialyzed enzyme into agar- agar solution. Agar solution was prepared in distilled water by vigorous shaking at 100°C, autoclaved and was allowed to cool to 40–45°C. Afterwards, enzyme was incorporated and mixed thoroughly. This mixture was immediately poured into a clean Petri plate and left to solidify at room temperature. Polymer films with and without immobilized DS were analyzed in terms of enzyme activity and reusability and mechanical properties (tensile strength, elongation at break and elastic modulus). In order to remove un-entrapped enzyme the films were washed with double deionized water and sodium acetate buffer (pH 5.4) three times prior to enzymatic assay. For quantitative analysis of samples for OS production a Dionex Ultimate 3000 HPLC system was used. 
Results showed that maximum immobilization yield (74.11%) was achieved by use of 2 % agar and (1:9) enzyme: agar ratio. HPLC analysis confirmed the similar trend of OS formation in immobilized samples compared to free enzyme. The lowest tested fraction of enzyme immobilized into polymer matrix (1:9) improved tensile strength of films in comparison with control film, whereas higher concentration of enzyme led to decrease in mechanical resistance of films. Scanning electron microscopy of agar films before and after DS entrapment revealed significant morphological change on the matrix surface. Considering the economic feasibility, the entrapped DS indicated imperative recycling efficiency up to six reaction cycles. The results of this study revealed that an easily available and inexpensive matrix could be successfully employed for DS immobilization and OS production.
C3  - PolyChar 26 World Forum on Advanced Materials
T1  - DEXTRANSUCRASE ENTRAPMENT AS AN EFFICIENT ALTERNATIVE FOR INCREASED RECYCLING EFFICIENCY OF FREE ENZYME WITHIN AGAR-AGAR FILM MATRIX
SP  - 88
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6239
ER  - 

@conference{
author = "Miljković, Miona and Davidović, Slađana and Radovanović, Neda and Gordić, Milan and Carević, Milica and Nešić, Aleksandra and Dimitrijević, Suzana",
year = "2018",
abstract = "Dextransucrase (DS), the extracellular enzyme is of immense industrial importance, due to ability to produce dextran and oligosaccharides (OS). Worldwide interest in OS has been increasing, since they have been accorded the prebiotic status. However, the industrial application of DS for OS synthesis is limited, due to low yield of enzyme production and its low catalytic activity. Hence, there is a great interest for development of new technologies that can provide improved performance of biocatalyst. Enzyme immobilization technology is considered to be a crucial step for cheaper and more efficient usage of DS. Entrapment is one of the widely investigated immobilization methods, where enzymes are enclosed or confined within the polymer matrix without altering their native structure, developing bioreactors for commercial applications. Different matrices such as polyacryl-amide gel, alginate beads and agar–agar have been used for the entrapment of different enzymes and among them agar–agar is a biocompatible, non-toxic and strong solidifying agent for immobilization of various enzymes.
In this work, the entrapment of DS was initiated by different quantity (1:9, 1:4 and 1:1) of dialyzed enzyme into agar- agar solution. Agar solution was prepared in distilled water by vigorous shaking at 100°C, autoclaved and was allowed to cool to 40–45°C. Afterwards, enzyme was incorporated and mixed thoroughly. This mixture was immediately poured into a clean Petri plate and left to solidify at room temperature. Polymer films with and without immobilized DS were analyzed in terms of enzyme activity and reusability and mechanical properties (tensile strength, elongation at break and elastic modulus). In order to remove un-entrapped enzyme the films were washed with double deionized water and sodium acetate buffer (pH 5.4) three times prior to enzymatic assay. For quantitative analysis of samples for OS production a Dionex Ultimate 3000 HPLC system was used. 
Results showed that maximum immobilization yield (74.11%) was achieved by use of 2 % agar and (1:9) enzyme: agar ratio. HPLC analysis confirmed the similar trend of OS formation in immobilized samples compared to free enzyme. The lowest tested fraction of enzyme immobilized into polymer matrix (1:9) improved tensile strength of films in comparison with control film, whereas higher concentration of enzyme led to decrease in mechanical resistance of films. Scanning electron microscopy of agar films before and after DS entrapment revealed significant morphological change on the matrix surface. Considering the economic feasibility, the entrapped DS indicated imperative recycling efficiency up to six reaction cycles. The results of this study revealed that an easily available and inexpensive matrix could be successfully employed for DS immobilization and OS production.",
journal = "PolyChar 26 World Forum on Advanced Materials",
title = "DEXTRANSUCRASE ENTRAPMENT AS AN EFFICIENT ALTERNATIVE FOR INCREASED RECYCLING EFFICIENCY OF FREE ENZYME WITHIN AGAR-AGAR FILM MATRIX",
pages = "88",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6239"
}

Miljković, M., Davidović, S., Radovanović, N., Gordić, M., Carević, M., Nešić, A.,& Dimitrijević, S.. (2018). DEXTRANSUCRASE ENTRAPMENT AS AN EFFICIENT ALTERNATIVE FOR INCREASED RECYCLING EFFICIENCY OF FREE ENZYME WITHIN AGAR-AGAR FILM MATRIX. in PolyChar 26 World Forum on Advanced Materials, 88.
https://hdl.handle.net/21.15107/rcub_technorep_6239

Miljković M, Davidović S, Radovanović N, Gordić M, Carević M, Nešić A, Dimitrijević S. DEXTRANSUCRASE ENTRAPMENT AS AN EFFICIENT ALTERNATIVE FOR INCREASED RECYCLING EFFICIENCY OF FREE ENZYME WITHIN AGAR-AGAR FILM MATRIX. in PolyChar 26 World Forum on Advanced Materials. 2018;:88.
https://hdl.handle.net/21.15107/rcub_technorep_6239 .

Miljković, Miona, Davidović, Slađana, Radovanović, Neda, Gordić, Milan, Carević, Milica, Nešić, Aleksandra, Dimitrijević, Suzana, "DEXTRANSUCRASE ENTRAPMENT AS AN EFFICIENT ALTERNATIVE FOR INCREASED RECYCLING EFFICIENCY OF FREE ENZYME WITHIN AGAR-AGAR FILM MATRIX" in PolyChar 26 World Forum on Advanced Materials (2018):88,
https://hdl.handle.net/21.15107/rcub_technorep_6239 .

TY  - JOUR
AU  - Radovanović, Neda
AU  - Davidović, Slađana
AU  - Miljković, Miona
AU  - Pavlović, Marija
AU  - Buntić, Aneta
AU  - Lazić, Vesna
AU  - Mihajlovski, Katarina
PY  - 2018
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3815
AB  - Amylases are industrially important enzymes which could convert starch to glucose, maltose and oligosaccharides. A bacterial strain designated as Paenibacillus chitinolyticus CKS1which was isolated from the soil of the coniferous forest, produced β-amylases using different commercial and waste substrates. Maximum β-amylases activity of 0.820 U/mL was obtained using a sugar alcohol-isomaltidex (0.5% w/v), as a substrate for microorganism growth and enzyme production. After 48 h of fermentation in a medium that contained starch (0.5%, w/v) and 0.05% v/v of ethanol, CKS1 produced β-amylase with the activity of 0.518 U/mL. The latest trends in enzyme production include utilisation of various waste products, mainly of agroindustrial origin, as a substrate for microorganisms growth. The strain CKS1 was also able to grow and produce β-amylases by using plant waste material. The plant waste substrate (PWS) contained plant biomass that is left after the ethanol extraction of various medicinal herbs (marigold and chamomile flowers, artichoke leaf, lemon balm leaf, nettle leaf, thyme leaf, yarrow shoot, yellow gentian shoot, primrose shoot, valerian shoot and chestnut and hawthorn seeds).This mixture of dried plant biomass is dissposed as such as a waste. In a medium with 0.1% (w/v) of PWS, CKS1 produced β-amylases with a maximum activity of 0.569 U/mL. The results show the potential of utilising waste plant biomass, left after ethanol extraction of medicinal herbs, in production of amylases. The application of microorganisms in β-amylase production using waste substrate is economically and environmentally accepted.
AB  - Amilaze predstavljaju grupu industrijski veoma važnih enzima koji hidrolizuju skrob do glukoze, maltoze i različitih oligosaharida. Bakterijski izolat Paenibacillus chitinolyticus CKS1, izolovan iz zemljišta četinarske šume, pokazao je sposobnost proizvodnje β-amilaza tokom svog rasta na različitim komercijalnim ali i na otpadnim supstratima. Maksimalna aktivnosti β-amilaza koja je iznosila 0,820 U/mL postignuta je korišćenjem izomaltideksa, šećernog alkohola, u koncentraciji od 0,5% (w/v), kao supstrata za rast mikroorganizma i proizvodnju enzima. U podlozi sa skrobom (0,5% w/v) i sa dodatkom 0,05% (v/v) etanola, nakon 48h fermentacije, maksimum aktivnosti β-amilaza iznosila je 0,518 U/mL. Najnoviji trendovi u proizvodnji enzima odnose se na korišćenje različitih otpadnih sirovina agroindustrijskog porekla kao supstrata za rast mikroorganizma. Soj CKS1 je pokazao mogućnost korišćenja otpadnog biljnog materijala kao supstrata za rast i proizvodnju enzima amilaza. Otpadni biljni supstrat (PWS), činila je biljna masa zaostala nakon etanolne ekstrakcije različitog lekovitog bilja (cveta nevena i kamilice, lista matičnjaka, artičoke, koprive, timijana, nadzemnog dela hajdučke trave, lincure, jagorčevine i valerijane i semena divljeg kestena i gloga). Ova biljna biomasa, iz koje su ekstrahovane bioaktivne materije, meša se i odlaže kao takva u vidu otpada. Nakon rasta u podlozi sa 1,0 % (w/v) PWS, soj CKS1 proizveo je β-amilaze sa aktivnošću od 0,569 U/mL. Ovi reziltati ukazuju na mogućnost iskorišćenja otpadne biljne biomase, zaostale nakon alkoholne ekstrakcije lekovitog bilja, u procesima ekonomičnije proizvodnje amilaza. Korišćenje otpadne sirovine u mikrobnim procesima proizvodnje enzima je i ekološki mnogo prihvatljivije usled uticaja na smanjene skladištenja otpada a samim tim i sveukupnog zagađenje životne sredine.
PB  - Nacionalno društvo za procesnu tehniku i energetiku u poljoprivredi, Novi Sad
T2  - Journal on Processing and Energy in Agriculture
T1  - β-amylase production by a novel strain Paenibacillus chitinolyticus CKS1 using commercial and waste substrates
T1  - Proizvodnja β-amilaza pomoću novog bakterijskog soja Paenibacillus chitinolyticus CKS1 na komercijalnim i otpadnim supstratima
EP  - 22
IS  - 1
SP  - 18
VL  - 22
DO  - 10.5937/JPEA1801018R
ER  - 

@article{
author = "Radovanović, Neda and Davidović, Slađana and Miljković, Miona and Pavlović, Marija and Buntić, Aneta and Lazić, Vesna and Mihajlovski, Katarina",
year = "2018",
abstract = "Amylases are industrially important enzymes which could convert starch to glucose, maltose and oligosaccharides. A bacterial strain designated as Paenibacillus chitinolyticus CKS1which was isolated from the soil of the coniferous forest, produced β-amylases using different commercial and waste substrates. Maximum β-amylases activity of 0.820 U/mL was obtained using a sugar alcohol-isomaltidex (0.5% w/v), as a substrate for microorganism growth and enzyme production. After 48 h of fermentation in a medium that contained starch (0.5%, w/v) and 0.05% v/v of ethanol, CKS1 produced β-amylase with the activity of 0.518 U/mL. The latest trends in enzyme production include utilisation of various waste products, mainly of agroindustrial origin, as a substrate for microorganisms growth. The strain CKS1 was also able to grow and produce β-amylases by using plant waste material. The plant waste substrate (PWS) contained plant biomass that is left after the ethanol extraction of various medicinal herbs (marigold and chamomile flowers, artichoke leaf, lemon balm leaf, nettle leaf, thyme leaf, yarrow shoot, yellow gentian shoot, primrose shoot, valerian shoot and chestnut and hawthorn seeds).This mixture of dried plant biomass is dissposed as such as a waste. In a medium with 0.1% (w/v) of PWS, CKS1 produced β-amylases with a maximum activity of 0.569 U/mL. The results show the potential of utilising waste plant biomass, left after ethanol extraction of medicinal herbs, in production of amylases. The application of microorganisms in β-amylase production using waste substrate is economically and environmentally accepted., Amilaze predstavljaju grupu industrijski veoma važnih enzima koji hidrolizuju skrob do glukoze, maltoze i različitih oligosaharida. Bakterijski izolat Paenibacillus chitinolyticus CKS1, izolovan iz zemljišta četinarske šume, pokazao je sposobnost proizvodnje β-amilaza tokom svog rasta na različitim komercijalnim ali i na otpadnim supstratima. Maksimalna aktivnosti β-amilaza koja je iznosila 0,820 U/mL postignuta je korišćenjem izomaltideksa, šećernog alkohola, u koncentraciji od 0,5% (w/v), kao supstrata za rast mikroorganizma i proizvodnju enzima. U podlozi sa skrobom (0,5% w/v) i sa dodatkom 0,05% (v/v) etanola, nakon 48h fermentacije, maksimum aktivnosti β-amilaza iznosila je 0,518 U/mL. Najnoviji trendovi u proizvodnji enzima odnose se na korišćenje različitih otpadnih sirovina agroindustrijskog porekla kao supstrata za rast mikroorganizma. Soj CKS1 je pokazao mogućnost korišćenja otpadnog biljnog materijala kao supstrata za rast i proizvodnju enzima amilaza. Otpadni biljni supstrat (PWS), činila je biljna masa zaostala nakon etanolne ekstrakcije različitog lekovitog bilja (cveta nevena i kamilice, lista matičnjaka, artičoke, koprive, timijana, nadzemnog dela hajdučke trave, lincure, jagorčevine i valerijane i semena divljeg kestena i gloga). Ova biljna biomasa, iz koje su ekstrahovane bioaktivne materije, meša se i odlaže kao takva u vidu otpada. Nakon rasta u podlozi sa 1,0 % (w/v) PWS, soj CKS1 proizveo je β-amilaze sa aktivnošću od 0,569 U/mL. Ovi reziltati ukazuju na mogućnost iskorišćenja otpadne biljne biomase, zaostale nakon alkoholne ekstrakcije lekovitog bilja, u procesima ekonomičnije proizvodnje amilaza. Korišćenje otpadne sirovine u mikrobnim procesima proizvodnje enzima je i ekološki mnogo prihvatljivije usled uticaja na smanjene skladištenja otpada a samim tim i sveukupnog zagađenje životne sredine.",
publisher = "Nacionalno društvo za procesnu tehniku i energetiku u poljoprivredi, Novi Sad",
journal = "Journal on Processing and Energy in Agriculture",
title = "β-amylase production by a novel strain Paenibacillus chitinolyticus CKS1 using commercial and waste substrates, Proizvodnja β-amilaza pomoću novog bakterijskog soja Paenibacillus chitinolyticus CKS1 na komercijalnim i otpadnim supstratima",
pages = "22-18",
number = "1",
volume = "22",
doi = "10.5937/JPEA1801018R"
}

Radovanović, N., Davidović, S., Miljković, M., Pavlović, M., Buntić, A., Lazić, V.,& Mihajlovski, K.. (2018). β-amylase production by a novel strain Paenibacillus chitinolyticus CKS1 using commercial and waste substrates. in Journal on Processing and Energy in Agriculture
Nacionalno društvo za procesnu tehniku i energetiku u poljoprivredi, Novi Sad., 22(1), 18-22.
https://doi.org/10.5937/JPEA1801018R

Radovanović N, Davidović S, Miljković M, Pavlović M, Buntić A, Lazić V, Mihajlovski K. β-amylase production by a novel strain Paenibacillus chitinolyticus CKS1 using commercial and waste substrates. in Journal on Processing and Energy in Agriculture. 2018;22(1):18-22.
doi:10.5937/JPEA1801018R .

Radovanović, Neda, Davidović, Slađana, Miljković, Miona, Pavlović, Marija, Buntić, Aneta, Lazić, Vesna, Mihajlovski, Katarina, "β-amylase production by a novel strain Paenibacillus chitinolyticus CKS1 using commercial and waste substrates" in Journal on Processing and Energy in Agriculture, 22, no. 1 (2018):18-22,
https://doi.org/10.5937/JPEA1801018R . .

TY  - JOUR
AU  - Davidović, Slađana
AU  - Miljković, Miona
AU  - Radovanović, Neda
AU  - Dimitrijević, Suzana
AU  - Nešić, Aleksandra
PY  - 2017
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6183
AB  - In this work, antimicrobial agar based nanocomposite films containing silver nanoparticles were prepared. For the synthesis, a masterbatch of in situ stabilized silver nanoparticles produced in agar solution was used. The incorporation of silver nanoparticles improved the mechanical and water vapor barrier resistance of films, in comparison to the neat agar film without silver. Agar/Ag films showed high antimicrobial activity against Staphylococcus aureus, Escherichia coli and Candida albicans. The migration of silver nanoparticles from the agar films, assessed by food contact tests, was less than 0.05 mg/g which is under the legal limits. These results indicated that the agar films incorporated with silver nanoparticles have potential to be used as packaging material.
T2  - Journal of International Scientific Publications, Agriculture & Food
T1  - PHYSICO-CHEMICAL PROPERTIES OF AGAR/SILVER NANOCOMPOSITE FILMS INTENDED FOR FOOD PACKAGING APPLICATION
EP  - 146
SP  - 139
VL  - 5
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6183
ER  - 

@article{
author = "Davidović, Slađana and Miljković, Miona and Radovanović, Neda and Dimitrijević, Suzana and Nešić, Aleksandra",
year = "2017",
abstract = "In this work, antimicrobial agar based nanocomposite films containing silver nanoparticles were prepared. For the synthesis, a masterbatch of in situ stabilized silver nanoparticles produced in agar solution was used. The incorporation of silver nanoparticles improved the mechanical and water vapor barrier resistance of films, in comparison to the neat agar film without silver. Agar/Ag films showed high antimicrobial activity against Staphylococcus aureus, Escherichia coli and Candida albicans. The migration of silver nanoparticles from the agar films, assessed by food contact tests, was less than 0.05 mg/g which is under the legal limits. These results indicated that the agar films incorporated with silver nanoparticles have potential to be used as packaging material.",
journal = "Journal of International Scientific Publications, Agriculture & Food",
title = "PHYSICO-CHEMICAL PROPERTIES OF AGAR/SILVER NANOCOMPOSITE FILMS INTENDED FOR FOOD PACKAGING APPLICATION",
pages = "146-139",
volume = "5",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6183"
}

Davidović, S., Miljković, M., Radovanović, N., Dimitrijević, S.,& Nešić, A.. (2017). PHYSICO-CHEMICAL PROPERTIES OF AGAR/SILVER NANOCOMPOSITE FILMS INTENDED FOR FOOD PACKAGING APPLICATION. in Journal of International Scientific Publications, Agriculture & Food, 5, 139-146.
https://hdl.handle.net/21.15107/rcub_technorep_6183

Davidović S, Miljković M, Radovanović N, Dimitrijević S, Nešić A. PHYSICO-CHEMICAL PROPERTIES OF AGAR/SILVER NANOCOMPOSITE FILMS INTENDED FOR FOOD PACKAGING APPLICATION. in Journal of International Scientific Publications, Agriculture & Food. 2017;5:139-146.
https://hdl.handle.net/21.15107/rcub_technorep_6183 .

Davidović, Slađana, Miljković, Miona, Radovanović, Neda, Dimitrijević, Suzana, Nešić, Aleksandra, "PHYSICO-CHEMICAL PROPERTIES OF AGAR/SILVER NANOCOMPOSITE FILMS INTENDED FOR FOOD PACKAGING APPLICATION" in Journal of International Scientific Publications, Agriculture & Food, 5 (2017):139-146,
https://hdl.handle.net/21.15107/rcub_technorep_6183 .

TY  - JOUR
AU  - Miljković, Miona
AU  - Davidović, Slađana
AU  - Davidović, Slađana
AU  - Radovanović, Neda
AU  - Dimitrijević, Suzana
PY  - 2017
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6247
AB  - In this work, the pectin/oregano oil nanoemulsion coatings were investigated, in order to prolong the shelf-life of cheese. The water vapor barrier, mechanical and antimicrobial activity of prepared nanoemulsion coatings were examined. In addition, the textural properties of uncoated and pectin/oregano oil coated cheeses were followed for 2 weeks. The results showed that obtained coatings possessed high antimicrobial activity toward E. coli, S. aureus and C. albicans and satisfied mechanical and water vapor barrier resistance. The coatings were also effective on reducing losses of weight and firmness of cheeses. Overall, antimicrobial nanoemulsion/edible films from renewable sources were successfully produced and showed high potential to be applicable in food industry to prolong the shelf-life of cheeses.
PB  - Sciences Invest
T2  - Journal of International Scientific Publications Agriculture & Food
T1  - The use of nanoemulsion-based edible coatings to prolong the shelflife of cheese
EP  - 138
SP  - 131
VL  - 5
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6247
ER  - 

@article{
author = "Miljković, Miona and Davidović, Slađana and Davidović, Slađana and Radovanović, Neda and Dimitrijević, Suzana",
year = "2017",
abstract = "In this work, the pectin/oregano oil nanoemulsion coatings were investigated, in order to prolong the shelf-life of cheese. The water vapor barrier, mechanical and antimicrobial activity of prepared nanoemulsion coatings were examined. In addition, the textural properties of uncoated and pectin/oregano oil coated cheeses were followed for 2 weeks. The results showed that obtained coatings possessed high antimicrobial activity toward E. coli, S. aureus and C. albicans and satisfied mechanical and water vapor barrier resistance. The coatings were also effective on reducing losses of weight and firmness of cheeses. Overall, antimicrobial nanoemulsion/edible films from renewable sources were successfully produced and showed high potential to be applicable in food industry to prolong the shelf-life of cheeses.",
publisher = "Sciences Invest",
journal = "Journal of International Scientific Publications Agriculture & Food",
title = "The use of nanoemulsion-based edible coatings to prolong the shelflife of cheese",
pages = "138-131",
volume = "5",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6247"
}

Miljković, M., Davidović, S., Davidović, S., Radovanović, N.,& Dimitrijević, S.. (2017). The use of nanoemulsion-based edible coatings to prolong the shelflife of cheese. in Journal of International Scientific Publications Agriculture & Food
Sciences Invest., 5, 131-138.
https://hdl.handle.net/21.15107/rcub_technorep_6247

Miljković M, Davidović S, Davidović S, Radovanović N, Dimitrijević S. The use of nanoemulsion-based edible coatings to prolong the shelflife of cheese. in Journal of International Scientific Publications Agriculture & Food. 2017;5:131-138.
https://hdl.handle.net/21.15107/rcub_technorep_6247 .

Miljković, Miona, Davidović, Slađana, Davidović, Slađana, Radovanović, Neda, Dimitrijević, Suzana, "The use of nanoemulsion-based edible coatings to prolong the shelflife of cheese" in Journal of International Scientific Publications Agriculture & Food, 5 (2017):131-138,
https://hdl.handle.net/21.15107/rcub_technorep_6247 .

TY  - JOUR
AU  - Miljković, Miona
AU  - Davidović, Slađana
AU  - Kralj, Slavko
AU  - Šiler-Marinković, Slavica
AU  - Rajilić-Stojanović, Mirjana
AU  - Dimitrijević-Branković, Suzana
PY  - 2017
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3632
AB  - The production of dextransucrase (DS) by Leuconostoc mesenteroides T3, novel isolate from water kefir grain, was studied and optimized. Bacterial supernatant reached activity of 3.1 U/ml when the culture was grown at 23 degrees C and under static culture condition using classical Tsuchiya medium for DS production. The increase of sucrose concentration to 7% led to an increase of DS activity by 52% compared to the control. Medium with 2% beef extract and 1% yeast extract resulted in 4.52 U/ml, which was 47% higher than in the control (with 2% yeast extract). Finally, the increase of K2HPO4 concentration from 2 to 3% resulted in the increased enzyme activity by 28%. Enzyme purified by polyethylene glycol 400 fractionation displayed maximum activity at 30 degrees C and pH 5.4. Zymogram analysis confirmed the presence of DS of approximately 180 kDa. The addition of divalent cations Ca2+, Mg2+, Fe2+ and Co2+ led to a minor increase of DS activity, while the addition of Mn2+ was the most prominent with 73% increase. These findings classify dextransucrase from Leuconostoc mesenteroides T3 as promising candidate for production of dextran, which has numerous applications in various industries.
PB  - Savez hemijskih inženjera, Beograd
T2  - Hemijska industrija
T1  - Characterization of dextransucrase from Leuconostoc mesenteroides T3, water kefir grains isolate
EP  - 360
IS  - 4
SP  - 351
VL  - 71
DO  - 10.2298/HEMIND160421046M
ER  - 

@article{
author = "Miljković, Miona and Davidović, Slađana and Kralj, Slavko and Šiler-Marinković, Slavica and Rajilić-Stojanović, Mirjana and Dimitrijević-Branković, Suzana",
year = "2017",
abstract = "The production of dextransucrase (DS) by Leuconostoc mesenteroides T3, novel isolate from water kefir grain, was studied and optimized. Bacterial supernatant reached activity of 3.1 U/ml when the culture was grown at 23 degrees C and under static culture condition using classical Tsuchiya medium for DS production. The increase of sucrose concentration to 7% led to an increase of DS activity by 52% compared to the control. Medium with 2% beef extract and 1% yeast extract resulted in 4.52 U/ml, which was 47% higher than in the control (with 2% yeast extract). Finally, the increase of K2HPO4 concentration from 2 to 3% resulted in the increased enzyme activity by 28%. Enzyme purified by polyethylene glycol 400 fractionation displayed maximum activity at 30 degrees C and pH 5.4. Zymogram analysis confirmed the presence of DS of approximately 180 kDa. The addition of divalent cations Ca2+, Mg2+, Fe2+ and Co2+ led to a minor increase of DS activity, while the addition of Mn2+ was the most prominent with 73% increase. These findings classify dextransucrase from Leuconostoc mesenteroides T3 as promising candidate for production of dextran, which has numerous applications in various industries.",
publisher = "Savez hemijskih inženjera, Beograd",
journal = "Hemijska industrija",
title = "Characterization of dextransucrase from Leuconostoc mesenteroides T3, water kefir grains isolate",
pages = "360-351",
number = "4",
volume = "71",
doi = "10.2298/HEMIND160421046M"
}

Miljković, M., Davidović, S., Kralj, S., Šiler-Marinković, S., Rajilić-Stojanović, M.,& Dimitrijević-Branković, S.. (2017). Characterization of dextransucrase from Leuconostoc mesenteroides T3, water kefir grains isolate. in Hemijska industrija
Savez hemijskih inženjera, Beograd., 71(4), 351-360.
https://doi.org/10.2298/HEMIND160421046M

Miljković M, Davidović S, Kralj S, Šiler-Marinković S, Rajilić-Stojanović M, Dimitrijević-Branković S. Characterization of dextransucrase from Leuconostoc mesenteroides T3, water kefir grains isolate. in Hemijska industrija. 2017;71(4):351-360.
doi:10.2298/HEMIND160421046M .

Miljković, Miona, Davidović, Slađana, Kralj, Slavko, Šiler-Marinković, Slavica, Rajilić-Stojanović, Mirjana, Dimitrijević-Branković, Suzana, "Characterization of dextransucrase from Leuconostoc mesenteroides T3, water kefir grains isolate" in Hemijska industrija, 71, no. 4 (2017):351-360,
https://doi.org/10.2298/HEMIND160421046M . .

TY  - JOUR
AU  - Nešić, Aleksandra
AU  - Gordić, Milan V.
AU  - Onjia, Antonije
AU  - Davidović, Slađana
AU  - Miljković, Miona
AU  - Dimitrijević-Branković, Suzana
PY  - 2017
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3665
AB  - In this work, antimicrobial bioinspired films made from chitosan incorporated with triclosan were investigated. The tensile strenght of these films were in the range of 33 and 39 MPa, which presented satisfied mechanical stability comparable to the synthetic-based packages commonly used in industry. The addition of triclosan enhanced thermal stability and antimicrobial activity of chitosan films against Escherichia coli and Staphylococus aureus. Results obtained in this work demonstrated that chitosan/triclosan films could be potentially used as an eco-sustainable package in healthcare sector to prevent infections/contaminations.
PB  - Elsevier, Amsterdam
T2  - Materials Letters
T1  - Chitosan-triclosan films for potential use as bio-antimicrobial bags in healthcare sector
EP  - 371
SP  - 368
VL  - 186
DO  - 10.1016/j.matlet.2016.10.028
ER  - 

@article{
author = "Nešić, Aleksandra and Gordić, Milan V. and Onjia, Antonije and Davidović, Slađana and Miljković, Miona and Dimitrijević-Branković, Suzana",
year = "2017",
abstract = "In this work, antimicrobial bioinspired films made from chitosan incorporated with triclosan were investigated. The tensile strenght of these films were in the range of 33 and 39 MPa, which presented satisfied mechanical stability comparable to the synthetic-based packages commonly used in industry. The addition of triclosan enhanced thermal stability and antimicrobial activity of chitosan films against Escherichia coli and Staphylococus aureus. Results obtained in this work demonstrated that chitosan/triclosan films could be potentially used as an eco-sustainable package in healthcare sector to prevent infections/contaminations.",
publisher = "Elsevier, Amsterdam",
journal = "Materials Letters",
title = "Chitosan-triclosan films for potential use as bio-antimicrobial bags in healthcare sector",
pages = "371-368",
volume = "186",
doi = "10.1016/j.matlet.2016.10.028"
}

Nešić, A., Gordić, M. V., Onjia, A., Davidović, S., Miljković, M.,& Dimitrijević-Branković, S.. (2017). Chitosan-triclosan films for potential use as bio-antimicrobial bags in healthcare sector. in Materials Letters
Elsevier, Amsterdam., 186, 368-371.
https://doi.org/10.1016/j.matlet.2016.10.028

Nešić A, Gordić MV, Onjia A, Davidović S, Miljković M, Dimitrijević-Branković S. Chitosan-triclosan films for potential use as bio-antimicrobial bags in healthcare sector. in Materials Letters. 2017;186:368-371.
doi:10.1016/j.matlet.2016.10.028 .

Nešić, Aleksandra, Gordić, Milan V., Onjia, Antonije, Davidović, Slađana, Miljković, Miona, Dimitrijević-Branković, Suzana, "Chitosan-triclosan films for potential use as bio-antimicrobial bags in healthcare sector" in Materials Letters, 186 (2017):368-371,
https://doi.org/10.1016/j.matlet.2016.10.028 . .

TY  - JOUR
AU  - Krezović, Bojana D.
AU  - Miljković, Miona
AU  - Stojanović, Sanja T.
AU  - Najman, Stevo
AU  - Filipović, Jovanka
AU  - Tomić, Simonida
PY  - 2017
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3709
AB  - The aim of this work was to synthesize a series of semi-interpenetrating polymer networks (semi-IPNs) of 2-hydroxyethyl acrylate and itaconic acid, in the presence of poly(N-vinylpyrrolidone). Samples were synthesized by free radical copolymerization with constant PVP and variable IA content and characterized for structural, morphological, thermal, swelling, drug release, antibacterial and cytotoxic properties. The chemical structure of samples was confirmed by Fourier-transform infrared spectroscopy (FTIR). Scanning electron microscopy (SEM) was used to examine morphology of samples and glass transition temperatures were determined by differential scanning calorimetry (DCS). The pH and temperature sensitivity was confirmed by measuring the dependence of the degree of swelling on pH and temperature. All samples show volume phase transition temperature (VPTT) around 47 degrees C. Drug release profiles were investigated using vitamin B-3 as a model drug. The vitamin B-3 transport mechanism was studied by fitting experimental data to five different model equations and calculating the corresponding parameters. It was also observed that IA content has a marked influence on the release profiles of vitamin B-3, so the rate of drug release can be designed by changing the HEA/IA ratio. Cell viability, performed using MTT test, was high for all samples and all concentration of extract used. The antibacterial activity of hydrogels was determined against Pseudomonas aeruginosa. It turns out that IA content and time of exposure have an influence on the antibacterial potential. All samples showed satisfied cell viability. Due to these characteristics, P(HEA/IA)/PVP semi-IPNs represent interesting biomaterials for biomedical applications.
PB  - Inst Chemical Engineers, Rugby
T2  - Chemical Engineering Research & Design
T1  - Structural, thermal, mechanical, swelling, drug release, antibacterial and cytotoxic properties of P(HEMA)/PVP semi-IPN hydrogels
EP  - 380
SP  - 368
VL  - 121
DO  - 10.1016/j.cherd.2017.03.030
ER  - 

@article{
author = "Krezović, Bojana D. and Miljković, Miona and Stojanović, Sanja T. and Najman, Stevo and Filipović, Jovanka and Tomić, Simonida",
year = "2017",
abstract = "The aim of this work was to synthesize a series of semi-interpenetrating polymer networks (semi-IPNs) of 2-hydroxyethyl acrylate and itaconic acid, in the presence of poly(N-vinylpyrrolidone). Samples were synthesized by free radical copolymerization with constant PVP and variable IA content and characterized for structural, morphological, thermal, swelling, drug release, antibacterial and cytotoxic properties. The chemical structure of samples was confirmed by Fourier-transform infrared spectroscopy (FTIR). Scanning electron microscopy (SEM) was used to examine morphology of samples and glass transition temperatures were determined by differential scanning calorimetry (DCS). The pH and temperature sensitivity was confirmed by measuring the dependence of the degree of swelling on pH and temperature. All samples show volume phase transition temperature (VPTT) around 47 degrees C. Drug release profiles were investigated using vitamin B-3 as a model drug. The vitamin B-3 transport mechanism was studied by fitting experimental data to five different model equations and calculating the corresponding parameters. It was also observed that IA content has a marked influence on the release profiles of vitamin B-3, so the rate of drug release can be designed by changing the HEA/IA ratio. Cell viability, performed using MTT test, was high for all samples and all concentration of extract used. The antibacterial activity of hydrogels was determined against Pseudomonas aeruginosa. It turns out that IA content and time of exposure have an influence on the antibacterial potential. All samples showed satisfied cell viability. Due to these characteristics, P(HEA/IA)/PVP semi-IPNs represent interesting biomaterials for biomedical applications.",
publisher = "Inst Chemical Engineers, Rugby",
journal = "Chemical Engineering Research & Design",
title = "Structural, thermal, mechanical, swelling, drug release, antibacterial and cytotoxic properties of P(HEMA)/PVP semi-IPN hydrogels",
pages = "380-368",
volume = "121",
doi = "10.1016/j.cherd.2017.03.030"
}

Krezović, B. D., Miljković, M., Stojanović, S. T., Najman, S., Filipović, J.,& Tomić, S.. (2017). Structural, thermal, mechanical, swelling, drug release, antibacterial and cytotoxic properties of P(HEMA)/PVP semi-IPN hydrogels. in Chemical Engineering Research & Design
Inst Chemical Engineers, Rugby., 121, 368-380.
https://doi.org/10.1016/j.cherd.2017.03.030

Krezović BD, Miljković M, Stojanović ST, Najman S, Filipović J, Tomić S. Structural, thermal, mechanical, swelling, drug release, antibacterial and cytotoxic properties of P(HEMA)/PVP semi-IPN hydrogels. in Chemical Engineering Research & Design. 2017;121:368-380.
doi:10.1016/j.cherd.2017.03.030 .

Krezović, Bojana D., Miljković, Miona, Stojanović, Sanja T., Najman, Stevo, Filipović, Jovanka, Tomić, Simonida, "Structural, thermal, mechanical, swelling, drug release, antibacterial and cytotoxic properties of P(HEMA)/PVP semi-IPN hydrogels" in Chemical Engineering Research & Design, 121 (2017):368-380,
https://doi.org/10.1016/j.cherd.2017.03.030 . .

TY  - JOUR
AU  - Dojčilović, Radovan
AU  - Pajović, Jelena D.
AU  - Božanić, Dušan K.
AU  - Bogdanović, Una
AU  - Vodnik, Vesna
AU  - Dimitrijević-Branković, Suzana
AU  - Miljković, Miona
AU  - Kascakova, Slavka
AU  - Refregiers, Matthieu
AU  - Đoković, Vladimir
PY  - 2017
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3726
AB  - The interaction of the tryptophan functionalized Ag nanoparticles and live Candida albicans cells was studied by synchrotron excitation deep-ultraviolet (DUV) fluorescence imaging at the DISCO beamline of Synchrotron SOLEIL. DUV imaging showed that incubation of the fungus with functionalized nanoparticles results in significant increase in the fluorescence signal. The analysis of the images revealed that the interaction of the nanoparticles with (pseudo)hyphae polymorphs of the diploid fungus was less pronounced than in the case of yeast cells or budding spores. The changes in the intensity of the fluorescence signals of the cells after incubation were followed in [327-353 nm] and [370-410 nm] spectral ranges that correspond to the fluorescence of tryptophan in non-polar and polar environment, respectively. As a consequence of the environmental sensitivity of the silver-tryptophan fluorescent nanoprobe, we were able to determine the possible accumulation sites of the nanoparticles. The analysis of the intensity decay kinetics showed that the photobleaching effects were more pronounced in the case of the functionalized nanoparticle treated cells. The results of time-integrated emission in the mentioned spectral ranges suggested that the nanoparticles penetrate the cells, but that the majority of the nanoparticles attach to the cells' surfaces.
PB  - Elsevier Science Bv, Amsterdam
T2  - Colloids and Surfaces B-Biointerfaces
T1  - Interaction of amino acid-functionalized silver nanoparticles and Candida albicans polymorphs: A deep-UV fluorescence imaging study
EP  - 348
SP  - 341
VL  - 155
DO  - 10.1016/j.colsurfb.2017.04.044
ER  - 

@article{
author = "Dojčilović, Radovan and Pajović, Jelena D. and Božanić, Dušan K. and Bogdanović, Una and Vodnik, Vesna and Dimitrijević-Branković, Suzana and Miljković, Miona and Kascakova, Slavka and Refregiers, Matthieu and Đoković, Vladimir",
year = "2017",
abstract = "The interaction of the tryptophan functionalized Ag nanoparticles and live Candida albicans cells was studied by synchrotron excitation deep-ultraviolet (DUV) fluorescence imaging at the DISCO beamline of Synchrotron SOLEIL. DUV imaging showed that incubation of the fungus with functionalized nanoparticles results in significant increase in the fluorescence signal. The analysis of the images revealed that the interaction of the nanoparticles with (pseudo)hyphae polymorphs of the diploid fungus was less pronounced than in the case of yeast cells or budding spores. The changes in the intensity of the fluorescence signals of the cells after incubation were followed in [327-353 nm] and [370-410 nm] spectral ranges that correspond to the fluorescence of tryptophan in non-polar and polar environment, respectively. As a consequence of the environmental sensitivity of the silver-tryptophan fluorescent nanoprobe, we were able to determine the possible accumulation sites of the nanoparticles. The analysis of the intensity decay kinetics showed that the photobleaching effects were more pronounced in the case of the functionalized nanoparticle treated cells. The results of time-integrated emission in the mentioned spectral ranges suggested that the nanoparticles penetrate the cells, but that the majority of the nanoparticles attach to the cells' surfaces.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Colloids and Surfaces B-Biointerfaces",
title = "Interaction of amino acid-functionalized silver nanoparticles and Candida albicans polymorphs: A deep-UV fluorescence imaging study",
pages = "348-341",
volume = "155",
doi = "10.1016/j.colsurfb.2017.04.044"
}

Dojčilović, R., Pajović, J. D., Božanić, D. K., Bogdanović, U., Vodnik, V., Dimitrijević-Branković, S., Miljković, M., Kascakova, S., Refregiers, M.,& Đoković, V.. (2017). Interaction of amino acid-functionalized silver nanoparticles and Candida albicans polymorphs: A deep-UV fluorescence imaging study. in Colloids and Surfaces B-Biointerfaces
Elsevier Science Bv, Amsterdam., 155, 341-348.
https://doi.org/10.1016/j.colsurfb.2017.04.044

Dojčilović R, Pajović JD, Božanić DK, Bogdanović U, Vodnik V, Dimitrijević-Branković S, Miljković M, Kascakova S, Refregiers M, Đoković V. Interaction of amino acid-functionalized silver nanoparticles and Candida albicans polymorphs: A deep-UV fluorescence imaging study. in Colloids and Surfaces B-Biointerfaces. 2017;155:341-348.
doi:10.1016/j.colsurfb.2017.04.044 .

Dojčilović, Radovan, Pajović, Jelena D., Božanić, Dušan K., Bogdanović, Una, Vodnik, Vesna, Dimitrijević-Branković, Suzana, Miljković, Miona, Kascakova, Slavka, Refregiers, Matthieu, Đoković, Vladimir, "Interaction of amino acid-functionalized silver nanoparticles and Candida albicans polymorphs: A deep-UV fluorescence imaging study" in Colloids and Surfaces B-Biointerfaces, 155 (2017):341-348,
https://doi.org/10.1016/j.colsurfb.2017.04.044 . .

TY  - CONF
AU  - Miljković, Miona
AU  - Davidović, Slađana
AU  - Nešić, Aleksandra
AU  - Mihajlovski, Katarina
AU  - Dimitrijević, Suzana
PY  - 2016
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6241
AB  - Dextransucrase (DS) is extracellular enzyme that catalyses the synthesis of dextran using
sucrose as a substrate, mainly produced by microorganisms belonging to the genera
Lactobacillus, Leuconostoc and Streptococcus. The aim of this study was usage of byproducts
from sugar refineries, molasses and sugar beet pulp (SBP) as a cheap substrate for
DS production. Fermentation process was performed by a natural isolate from water kefir
grain Leuconostoc mesenteroides T3. Molasses (ethanol Factory Alpis, Kovin, Serbia) with
addition of SBP (Fibrex 620, Nordic Sugar, Denmark) was used as fermentation media.
Molasses was diluted in distilledwater to a concentration of 2.5%. SBP was grounded in a
mortarand pestle to a particle size of 500–800μm. Four different treatments were
performed on the SBP before it was added into molasses: NaOH extraction, enzymatic
pretreatment (Viskozym), biological pretreatment (Paenibacillus chitinolyticus CKS1) and
combination of microwave and ultrasound treatment. The enzyme activity was determined
by measuring the concentration of released reducing sugars by DNS method at 540nm,
using fructose as a standard.Combination of microwave and ultrasound treatment as well
as biological pretreatment resulted in low DS activity. The best results of 1.45U/ml were
obtained with alkali and enzymatic pretreatments. Considering that enzymatic treatment
is environmental friendly it provides suitable method for SBP pretreatment for enhanced
DS production on molasses.
C3  - SOLID URBAN WASTE MANAGEMENT XXI IUPAC CHEMRAWN CONFERENCE
T1  - USAGE OF BY-PRODUCTS FROM SUGAR INDUSTRY AS A CHEAP SUBSTRATE FOR DEXTRANSUCRASE PRODUCTION Different treatments of sugar beet pulp for enhanced dextransucrase production on molasses
SP  - 77
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6241
ER  - 

@conference{
author = "Miljković, Miona and Davidović, Slađana and Nešić, Aleksandra and Mihajlovski, Katarina and Dimitrijević, Suzana",
year = "2016",
abstract = "Dextransucrase (DS) is extracellular enzyme that catalyses the synthesis of dextran using
sucrose as a substrate, mainly produced by microorganisms belonging to the genera
Lactobacillus, Leuconostoc and Streptococcus. The aim of this study was usage of byproducts
from sugar refineries, molasses and sugar beet pulp (SBP) as a cheap substrate for
DS production. Fermentation process was performed by a natural isolate from water kefir
grain Leuconostoc mesenteroides T3. Molasses (ethanol Factory Alpis, Kovin, Serbia) with
addition of SBP (Fibrex 620, Nordic Sugar, Denmark) was used as fermentation media.
Molasses was diluted in distilledwater to a concentration of 2.5%. SBP was grounded in a
mortarand pestle to a particle size of 500–800μm. Four different treatments were
performed on the SBP before it was added into molasses: NaOH extraction, enzymatic
pretreatment (Viskozym), biological pretreatment (Paenibacillus chitinolyticus CKS1) and
combination of microwave and ultrasound treatment. The enzyme activity was determined
by measuring the concentration of released reducing sugars by DNS method at 540nm,
using fructose as a standard.Combination of microwave and ultrasound treatment as well
as biological pretreatment resulted in low DS activity. The best results of 1.45U/ml were
obtained with alkali and enzymatic pretreatments. Considering that enzymatic treatment
is environmental friendly it provides suitable method for SBP pretreatment for enhanced
DS production on molasses.",
journal = "SOLID URBAN WASTE MANAGEMENT XXI IUPAC CHEMRAWN CONFERENCE",
title = "USAGE OF BY-PRODUCTS FROM SUGAR INDUSTRY AS A CHEAP SUBSTRATE FOR DEXTRANSUCRASE PRODUCTION Different treatments of sugar beet pulp for enhanced dextransucrase production on molasses",
pages = "77",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6241"
}

Miljković, M., Davidović, S., Nešić, A., Mihajlovski, K.,& Dimitrijević, S.. (2016). USAGE OF BY-PRODUCTS FROM SUGAR INDUSTRY AS A CHEAP SUBSTRATE FOR DEXTRANSUCRASE PRODUCTION Different treatments of sugar beet pulp for enhanced dextransucrase production on molasses. in SOLID URBAN WASTE MANAGEMENT XXI IUPAC CHEMRAWN CONFERENCE, 77.
https://hdl.handle.net/21.15107/rcub_technorep_6241

Miljković M, Davidović S, Nešić A, Mihajlovski K, Dimitrijević S. USAGE OF BY-PRODUCTS FROM SUGAR INDUSTRY AS A CHEAP SUBSTRATE FOR DEXTRANSUCRASE PRODUCTION Different treatments of sugar beet pulp for enhanced dextransucrase production on molasses. in SOLID URBAN WASTE MANAGEMENT XXI IUPAC CHEMRAWN CONFERENCE. 2016;:77.
https://hdl.handle.net/21.15107/rcub_technorep_6241 .

Miljković, Miona, Davidović, Slađana, Nešić, Aleksandra, Mihajlovski, Katarina, Dimitrijević, Suzana, "USAGE OF BY-PRODUCTS FROM SUGAR INDUSTRY AS A CHEAP SUBSTRATE FOR DEXTRANSUCRASE PRODUCTION Different treatments of sugar beet pulp for enhanced dextransucrase production on molasses" in SOLID URBAN WASTE MANAGEMENT XXI IUPAC CHEMRAWN CONFERENCE (2016):77,
https://hdl.handle.net/21.15107/rcub_technorep_6241 .

TY  - CONF
AU  - Davidović, Slađana
AU  - Miljković, Miona
AU  - Radovanović, Neda
AU  - Lazic, Vesna
AU  - Nešić, Aleksandra
AU  - Dimitrijević, Suzana
PY  - 2016
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6182
AB  - Antimicrobial potential of a film made from dextran incorporated with silver nanoparticles (Ag NPs) against Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus was investigated. First, the colloidal Ag NPs solutions of three different concentrations: 0.5, 1.0 and 2.0 mmol L-1 were made. The next step was preparation of dextran/nanosilver films. Disc diffusion method on agar and quantitative test in liquid medium were performed to evaluate the antibacterial activity of prepared composites. The results obtained in this work demonstrated that dextran-based film incorporated with Ag NPs could be used as a coating to prevent contaminations of working surfaces.
C3  - NANO FOR HEALTH
T1  - Antimicrobial properties of dextran-based coatings incorporated with silver nanoparticles
SP  - 137
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6182
ER  - 

@conference{
author = "Davidović, Slađana and Miljković, Miona and Radovanović, Neda and Lazic, Vesna and Nešić, Aleksandra and Dimitrijević, Suzana",
year = "2016",
abstract = "Antimicrobial potential of a film made from dextran incorporated with silver nanoparticles (Ag NPs) against Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus was investigated. First, the colloidal Ag NPs solutions of three different concentrations: 0.5, 1.0 and 2.0 mmol L-1 were made. The next step was preparation of dextran/nanosilver films. Disc diffusion method on agar and quantitative test in liquid medium were performed to evaluate the antibacterial activity of prepared composites. The results obtained in this work demonstrated that dextran-based film incorporated with Ag NPs could be used as a coating to prevent contaminations of working surfaces.",
journal = "NANO FOR HEALTH",
title = "Antimicrobial properties of dextran-based coatings incorporated with silver nanoparticles",
pages = "137",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6182"
}

Davidović, S., Miljković, M., Radovanović, N., Lazic, V., Nešić, A.,& Dimitrijević, S.. (2016). Antimicrobial properties of dextran-based coatings incorporated with silver nanoparticles. in NANO FOR HEALTH, 137.
https://hdl.handle.net/21.15107/rcub_technorep_6182

Davidović S, Miljković M, Radovanović N, Lazic V, Nešić A, Dimitrijević S. Antimicrobial properties of dextran-based coatings incorporated with silver nanoparticles. in NANO FOR HEALTH. 2016;:137.
https://hdl.handle.net/21.15107/rcub_technorep_6182 .

Davidović, Slađana, Miljković, Miona, Radovanović, Neda, Lazic, Vesna, Nešić, Aleksandra, Dimitrijević, Suzana, "Antimicrobial properties of dextran-based coatings incorporated with silver nanoparticles" in NANO FOR HEALTH (2016):137,
https://hdl.handle.net/21.15107/rcub_technorep_6182 .