TY  - CONF
AU  - Ilić, Nevena
AU  - Davidović, Slađana
AU  - Miljković, Miona
AU  - Radovanović, Neda
AU  - Dimitrijević-Branković, Suzana
AU  - Mihajlovski, Katarina
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6943
AB  - In recent decades, enzyme immobilization on different supports occupies an important place in the modern biotechnology, given that it allows the design of green and sustainable production processes. Nanoparticles are very efficient supports for enzyme immobilization. The enzyme immobilized on nanoparticles is more stable than its soluble form and can be used in several operative cycles. In this study, the potential of modified titanium dioxide nanoparticles (Degussa P25, TiO2) for crude fungal laccase (47.43 U/g) immobilization was investigated. The TiO2 nanoparticles were modified with (3-glycidyloxypropyl) trimetoxylane under different conditions in order to obtain support with an optimal concentration of epoxy groups for immobilization. The obtained TiO2 nanoparticles had different concentrations of epoxy groups on their surface, exactly 950 μmol/g (TiO2M1) and 500 μmol/g (TiO2M2), respectively. The immobilization was carried out at pH 5 and at room temperature for 4 h. The residual activity of immobilized laccase on TiO2M2 was 33.40 %, while the residual activity of immobilized laccase on TiO2M1 was 17.39 %. The immobilization efficiency was 26.75 % and 20.18 % for laccase immobilized on TiO2M2 and immobilized laccase on TiO2M1, respectively. The immobilization of crude fungal laccase on TiO2M2 was further optimized by testing the influence of different contact time between laccase and TiO2M2 (2 h ,3 h, 3,5 h and 4 h) as well as the effect of various pH values (pH 4, pH 5 and pH 6) on immobilization. The highest residual activity of 35.04 % and immobilization efficiency of 28.95 % were obtained for optimal contact time of 3.5 h between crude fungal laccase and TiO2M2. The lowest residual activity (30.38 %) and immobilization efficiency (20.18 %) were obtained when immobilization was carried out at room temperature and at pH 5 for 2 h. The pH values had significant effect on immobilization. The optimal pH value was pH 5 with highest residual activity of 35.04 %, while the lowest residual activity of 14. 83 % was at pH 6. The residual activity of 21.21 % and immobilization efficency of 19.52 % were obtained when crude fungal laccase immobilized at pH 4. The immobilized laccase on TiO2M2 was successfully used in 5 cycles of guaiacol oxidation. In the 2nd cycle, the immobilized laccase had residual activity of about 60%, while after 3rd cycle, immobilized laccase had residual activity of 40 %. The lowest residual activity of 14.83 %, immobilized laccase had after 5th cycle.
PB  - Zvornik : University of East Sarajevo Faculty of Technology
C3  - Proceedings / VIII International Congress "Engineering, Environment and Materials in Process Industry", EEM2023, Jahorina, March 20-23, 2023
T1  - IMMOBILIZATION OF CRUDE FUNGAL LACCASE FROM GANODERMA SPP. ON MODIFIED TITANIUM DIOXIDE NANOPARTICLES
EP  - 196
SP  - 187
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6943
ER  - 

@conference{
author = "Ilić, Nevena and Davidović, Slađana and Miljković, Miona and Radovanović, Neda and Dimitrijević-Branković, Suzana and Mihajlovski, Katarina",
year = "2023",
abstract = "In recent decades, enzyme immobilization on different supports occupies an important place in the modern biotechnology, given that it allows the design of green and sustainable production processes. Nanoparticles are very efficient supports for enzyme immobilization. The enzyme immobilized on nanoparticles is more stable than its soluble form and can be used in several operative cycles. In this study, the potential of modified titanium dioxide nanoparticles (Degussa P25, TiO2) for crude fungal laccase (47.43 U/g) immobilization was investigated. The TiO2 nanoparticles were modified with (3-glycidyloxypropyl) trimetoxylane under different conditions in order to obtain support with an optimal concentration of epoxy groups for immobilization. The obtained TiO2 nanoparticles had different concentrations of epoxy groups on their surface, exactly 950 μmol/g (TiO2M1) and 500 μmol/g (TiO2M2), respectively. The immobilization was carried out at pH 5 and at room temperature for 4 h. The residual activity of immobilized laccase on TiO2M2 was 33.40 %, while the residual activity of immobilized laccase on TiO2M1 was 17.39 %. The immobilization efficiency was 26.75 % and 20.18 % for laccase immobilized on TiO2M2 and immobilized laccase on TiO2M1, respectively. The immobilization of crude fungal laccase on TiO2M2 was further optimized by testing the influence of different contact time between laccase and TiO2M2 (2 h ,3 h, 3,5 h and 4 h) as well as the effect of various pH values (pH 4, pH 5 and pH 6) on immobilization. The highest residual activity of 35.04 % and immobilization efficiency of 28.95 % were obtained for optimal contact time of 3.5 h between crude fungal laccase and TiO2M2. The lowest residual activity (30.38 %) and immobilization efficiency (20.18 %) were obtained when immobilization was carried out at room temperature and at pH 5 for 2 h. The pH values had significant effect on immobilization. The optimal pH value was pH 5 with highest residual activity of 35.04 %, while the lowest residual activity of 14. 83 % was at pH 6. The residual activity of 21.21 % and immobilization efficency of 19.52 % were obtained when crude fungal laccase immobilized at pH 4. The immobilized laccase on TiO2M2 was successfully used in 5 cycles of guaiacol oxidation. In the 2nd cycle, the immobilized laccase had residual activity of about 60%, while after 3rd cycle, immobilized laccase had residual activity of 40 %. The lowest residual activity of 14.83 %, immobilized laccase had after 5th cycle.",
publisher = "Zvornik : University of East Sarajevo Faculty of Technology",
journal = "Proceedings / VIII International Congress "Engineering, Environment and Materials in Process Industry", EEM2023, Jahorina, March 20-23, 2023",
title = "IMMOBILIZATION OF CRUDE FUNGAL LACCASE FROM GANODERMA SPP. ON MODIFIED TITANIUM DIOXIDE NANOPARTICLES",
pages = "196-187",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6943"
}

Ilić, N., Davidović, S., Miljković, M., Radovanović, N., Dimitrijević-Branković, S.,& Mihajlovski, K.. (2023). IMMOBILIZATION OF CRUDE FUNGAL LACCASE FROM GANODERMA SPP. ON MODIFIED TITANIUM DIOXIDE NANOPARTICLES. in Proceedings / VIII International Congress "Engineering, Environment and Materials in Process Industry", EEM2023, Jahorina, March 20-23, 2023
Zvornik : University of East Sarajevo Faculty of Technology., 187-196.
https://hdl.handle.net/21.15107/rcub_technorep_6943

Ilić N, Davidović S, Miljković M, Radovanović N, Dimitrijević-Branković S, Mihajlovski K. IMMOBILIZATION OF CRUDE FUNGAL LACCASE FROM GANODERMA SPP. ON MODIFIED TITANIUM DIOXIDE NANOPARTICLES. in Proceedings / VIII International Congress "Engineering, Environment and Materials in Process Industry", EEM2023, Jahorina, March 20-23, 2023. 2023;:187-196.
https://hdl.handle.net/21.15107/rcub_technorep_6943 .

Ilić, Nevena, Davidović, Slađana, Miljković, Miona, Radovanović, Neda, Dimitrijević-Branković, Suzana, Mihajlovski, Katarina, "IMMOBILIZATION OF CRUDE FUNGAL LACCASE FROM GANODERMA SPP. ON MODIFIED TITANIUM DIOXIDE NANOPARTICLES" in Proceedings / VIII International Congress "Engineering, Environment and Materials in Process Industry", EEM2023, Jahorina, March 20-23, 2023 (2023):187-196,
https://hdl.handle.net/21.15107/rcub_technorep_6943 .

TY  - CONF
AU  - Ilić, Nevena
AU  - Lazić, Vesna
AU  - Radovanović, Neda
AU  - Mihajlovski, Katarina
AU  - Davidović, Slađana
AU  - Miljković, Miona
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5927
AB  - Silver and titanium dioxide nanoparticles (NPs) are the most produced NPs worldwide due to their antimicrobial properties. These NPs are widely used in many fields including agriculture, where they can inhibit the growth of plant pathogens and stimulate plant growth. However, there are concerns about nanoparticles reaching the environment, as they can also manifest adverse effects. In this study, the effects of TiO2 and two types of Ag NPs (synthesized by chemical reduction and “green” by dextrane) on soil microorganisms and organic beans were investigated. Two different species of rhizospheric soil microorganisms, namely actinomycetes Streptomyces microflavus and nitrogen-fixing soil bacteria Rhizobium sp. were exposed to the low concentration of NPs. For that purpose, microorganisms were grown in nutrient media supplemented with 0.2 mM NPs. After 24 h of exposure, the numbers of viable cells of both tested microorganisms were higher in the samples compared to the controls (media without NPs) for all three NPs, suggesting their stimulatory effect on the soil microorganisms' growth. The highest positive effect was observed for Rhizobium sp. treated with TiO2 NPs (the number of viable cells increased for two orders of magnitude, from ~106 to ~108 CFU/mL). Ag NPs showed equal influence on both tested microorganisms, with dextrane-coated Ag NPs were more effective. In the experiment with organic mung beans, the same colloid solutions of Ag NPs and TiO2 NPs had a stimulating effect on the germination and root length of this plant, also. The number of germinated seeds was the same as in the control, while the average root length was higher in the samples than in the control. The average root length was 1.47 cm in the control. Seeds treated with dextrane-coated Ag NPs had an average root length even 82 % higher compared to the control. The stimulatory effect was also observed in the case of seeds treated with Ag NPs synthesized by chemical reduction and TiO2 NPs, where the average root lengths were higher for 40% and 76 %, respectively than in the control. Germination index values, which are higher than 100%, suggesting that tested nanoparticles can be used as fertilizers in agriculture. Moreover, the obtained results show that silver nanoparticles and titanium dioxide nanoparticles have a stimulatory effect on the soil bacteria and organic mung bean, which prove their safety for this kind of application.
C3  - Proceedings,VII International Congress “ Engineering, Environment and Materials in Process Industry
T1  - Investigation of the influence of different nanoparticles on the growth of soil microorganisms and organic mung bean
EP  - 460
SP  - 451
UR  - https://hdl.handle.net/21.15107/rcub_technorep_5927
ER  - 

@conference{
author = "Ilić, Nevena and Lazić, Vesna and Radovanović, Neda and Mihajlovski, Katarina and Davidović, Slađana and Miljković, Miona",
year = "2021",
abstract = "Silver and titanium dioxide nanoparticles (NPs) are the most produced NPs worldwide due to their antimicrobial properties. These NPs are widely used in many fields including agriculture, where they can inhibit the growth of plant pathogens and stimulate plant growth. However, there are concerns about nanoparticles reaching the environment, as they can also manifest adverse effects. In this study, the effects of TiO2 and two types of Ag NPs (synthesized by chemical reduction and “green” by dextrane) on soil microorganisms and organic beans were investigated. Two different species of rhizospheric soil microorganisms, namely actinomycetes Streptomyces microflavus and nitrogen-fixing soil bacteria Rhizobium sp. were exposed to the low concentration of NPs. For that purpose, microorganisms were grown in nutrient media supplemented with 0.2 mM NPs. After 24 h of exposure, the numbers of viable cells of both tested microorganisms were higher in the samples compared to the controls (media without NPs) for all three NPs, suggesting their stimulatory effect on the soil microorganisms' growth. The highest positive effect was observed for Rhizobium sp. treated with TiO2 NPs (the number of viable cells increased for two orders of magnitude, from ~106 to ~108 CFU/mL). Ag NPs showed equal influence on both tested microorganisms, with dextrane-coated Ag NPs were more effective. In the experiment with organic mung beans, the same colloid solutions of Ag NPs and TiO2 NPs had a stimulating effect on the germination and root length of this plant, also. The number of germinated seeds was the same as in the control, while the average root length was higher in the samples than in the control. The average root length was 1.47 cm in the control. Seeds treated with dextrane-coated Ag NPs had an average root length even 82 % higher compared to the control. The stimulatory effect was also observed in the case of seeds treated with Ag NPs synthesized by chemical reduction and TiO2 NPs, where the average root lengths were higher for 40% and 76 %, respectively than in the control. Germination index values, which are higher than 100%, suggesting that tested nanoparticles can be used as fertilizers in agriculture. Moreover, the obtained results show that silver nanoparticles and titanium dioxide nanoparticles have a stimulatory effect on the soil bacteria and organic mung bean, which prove their safety for this kind of application.",
journal = "Proceedings,VII International Congress “ Engineering, Environment and Materials in Process Industry",
title = "Investigation of the influence of different nanoparticles on the growth of soil microorganisms and organic mung bean",
pages = "460-451",
url = "https://hdl.handle.net/21.15107/rcub_technorep_5927"
}

Ilić, N., Lazić, V., Radovanović, N., Mihajlovski, K., Davidović, S.,& Miljković, M.. (2021). Investigation of the influence of different nanoparticles on the growth of soil microorganisms and organic mung bean. in Proceedings,VII International Congress “ Engineering, Environment and Materials in Process Industry, 451-460.
https://hdl.handle.net/21.15107/rcub_technorep_5927

Ilić N, Lazić V, Radovanović N, Mihajlovski K, Davidović S, Miljković M. Investigation of the influence of different nanoparticles on the growth of soil microorganisms and organic mung bean. in Proceedings,VII International Congress “ Engineering, Environment and Materials in Process Industry. 2021;:451-460.
https://hdl.handle.net/21.15107/rcub_technorep_5927 .

Ilić, Nevena, Lazić, Vesna, Radovanović, Neda, Mihajlovski, Katarina, Davidović, Slađana, Miljković, Miona, "Investigation of the influence of different nanoparticles on the growth of soil microorganisms and organic mung bean" in Proceedings,VII International Congress “ Engineering, Environment and Materials in Process Industry (2021):451-460,
https://hdl.handle.net/21.15107/rcub_technorep_5927 .

TY  - JOUR
AU  - Cabrera-Barjas, Gustavo
AU  - Radovanović, Neda
AU  - Arrepol, Gaston Bravo
AU  - de la Torre, Alexander F.
AU  - Valdes, Oscar
AU  - Nesic, Aleksandra
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4785
AB  - The efficient use of waste from food processing industry is one of the innovative approaches within sustainable development, because it can be transferred into added value products, which could improve economic, energetic and environmental sectors. In this context, the squid pen waste from seafood industry was used as raw material to obtain nanofibrous beta-chitin films. In order to extend functionality of obtained films, elderberry extract obtained from biomass was added at different concentrations. The tensile strength of chitin-elderberry extract films was improved by 52%, elongation at break by 153% and water vapor barrier by 65%. The obtained material showed distinct color change when subjected to acidic or basic solutions. It was proven by CIELab color analysis that all color changes could be easily perceived visually. In addition, the obtained nanofibrous film was successfully used to monitor the freshness of Hake fish. Namely, when the film was introduced in a package that contained fresh fish, its color was efficiently changed within the time during the storage at 4 degrees C. The obtained results demonstrated that food processing waste could be efficiently valorized, and could give sustainable food package design as a spoilage indicator of high protein food.
T2  - International Journal of Biological Macromolecules
T1  - Valorization of food waste to produce intelligent nanofibrous beta-chitin films
EP  - 99
SP  - 92
VL  - 186
DO  - 10.1016/j.ijbiomac.2021.07.045
ER  - 

@article{
author = "Cabrera-Barjas, Gustavo and Radovanović, Neda and Arrepol, Gaston Bravo and de la Torre, Alexander F. and Valdes, Oscar and Nesic, Aleksandra",
year = "2021",
abstract = "The efficient use of waste from food processing industry is one of the innovative approaches within sustainable development, because it can be transferred into added value products, which could improve economic, energetic and environmental sectors. In this context, the squid pen waste from seafood industry was used as raw material to obtain nanofibrous beta-chitin films. In order to extend functionality of obtained films, elderberry extract obtained from biomass was added at different concentrations. The tensile strength of chitin-elderberry extract films was improved by 52%, elongation at break by 153% and water vapor barrier by 65%. The obtained material showed distinct color change when subjected to acidic or basic solutions. It was proven by CIELab color analysis that all color changes could be easily perceived visually. In addition, the obtained nanofibrous film was successfully used to monitor the freshness of Hake fish. Namely, when the film was introduced in a package that contained fresh fish, its color was efficiently changed within the time during the storage at 4 degrees C. The obtained results demonstrated that food processing waste could be efficiently valorized, and could give sustainable food package design as a spoilage indicator of high protein food.",
journal = "International Journal of Biological Macromolecules",
title = "Valorization of food waste to produce intelligent nanofibrous beta-chitin films",
pages = "99-92",
volume = "186",
doi = "10.1016/j.ijbiomac.2021.07.045"
}

Cabrera-Barjas, G., Radovanović, N., Arrepol, G. B., de la Torre, A. F., Valdes, O.,& Nesic, A.. (2021). Valorization of food waste to produce intelligent nanofibrous beta-chitin films. in International Journal of Biological Macromolecules, 186, 92-99.
https://doi.org/10.1016/j.ijbiomac.2021.07.045

Cabrera-Barjas G, Radovanović N, Arrepol GB, de la Torre AF, Valdes O, Nesic A. Valorization of food waste to produce intelligent nanofibrous beta-chitin films. in International Journal of Biological Macromolecules. 2021;186:92-99.
doi:10.1016/j.ijbiomac.2021.07.045 .

Cabrera-Barjas, Gustavo, Radovanović, Neda, Arrepol, Gaston Bravo, de la Torre, Alexander F., Valdes, Oscar, Nesic, Aleksandra, "Valorization of food waste to produce intelligent nanofibrous beta-chitin films" in International Journal of Biological Macromolecules, 186 (2021):92-99,
https://doi.org/10.1016/j.ijbiomac.2021.07.045 . .

TY  - JOUR
AU  - Nešić, Aleksandra
AU  - Cabrera-Barjas, Gustavo
AU  - Dimitrijević-Branković, Suzana
AU  - Davidović, Slađana
AU  - Radovanović, Neda
AU  - Delattre, Cédric
PY  - 2020
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4479
AB  - The use of polysaccharide-based materials presents an eco-friendly technological solution, by reducing dependence on fossil resources while reducing a product's carbon footprint, when compared to conventional plastic packaging materials. This review discusses the potential of polysaccharides as a raw material to produce multifunctional materials for food packaging applications. The covered areas include the recent innovations and properties of the polysaccharide-based materials. Emphasis is given to hemicelluloses, marine polysaccharides, and bacterial exopolysaccharides and their potential application in the latest trends of food packaging materials, including edible coatings, intelligent films, and thermo-insulated aerogel packaging.
PB  - MDPI, Basel
T2  - Molecules
T1  - Prospect of Polysaccharide-Based Materials as Advanced Food Packaging
IS  - 1
VL  - 25
DO  - 10.3390/molecules25010135
ER  - 

@article{
author = "Nešić, Aleksandra and Cabrera-Barjas, Gustavo and Dimitrijević-Branković, Suzana and Davidović, Slađana and Radovanović, Neda and Delattre, Cédric",
year = "2020",
abstract = "The use of polysaccharide-based materials presents an eco-friendly technological solution, by reducing dependence on fossil resources while reducing a product's carbon footprint, when compared to conventional plastic packaging materials. This review discusses the potential of polysaccharides as a raw material to produce multifunctional materials for food packaging applications. The covered areas include the recent innovations and properties of the polysaccharide-based materials. Emphasis is given to hemicelluloses, marine polysaccharides, and bacterial exopolysaccharides and their potential application in the latest trends of food packaging materials, including edible coatings, intelligent films, and thermo-insulated aerogel packaging.",
publisher = "MDPI, Basel",
journal = "Molecules",
title = "Prospect of Polysaccharide-Based Materials as Advanced Food Packaging",
number = "1",
volume = "25",
doi = "10.3390/molecules25010135"
}

Nešić, A., Cabrera-Barjas, G., Dimitrijević-Branković, S., Davidović, S., Radovanović, N.,& Delattre, C.. (2020). Prospect of Polysaccharide-Based Materials as Advanced Food Packaging. in Molecules
MDPI, Basel., 25(1).
https://doi.org/10.3390/molecules25010135

Nešić A, Cabrera-Barjas G, Dimitrijević-Branković S, Davidović S, Radovanović N, Delattre C. Prospect of Polysaccharide-Based Materials as Advanced Food Packaging. in Molecules. 2020;25(1).
doi:10.3390/molecules25010135 .

Nešić, Aleksandra, Cabrera-Barjas, Gustavo, Dimitrijević-Branković, Suzana, Davidović, Slađana, Radovanović, Neda, Delattre, Cédric, "Prospect of Polysaccharide-Based Materials as Advanced Food Packaging" in Molecules, 25, no. 1 (2020),
https://doi.org/10.3390/molecules25010135 . .

TY  - JOUR
AU  - Radovanović, Neda
AU  - Malagurski, Ivana
AU  - Lević, Steva
AU  - Gordić, Milan V.
AU  - Petrović, Jelena
AU  - Pavlović, Vladimir B.
AU  - Mitrić, Miodrag
AU  - Nešić, Aleksandra
AU  - Dimitrijević-Branković, Suzana
PY  - 2019
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4054
AB  - New agar-based composite films with increasing Cu-carbonate and Cu-phosphate mineral phase content were prepared by in situ mineralization and solvent casting method. SEM and optical analysis revealed that Cu-carbonate phase had better compatibility with agar matrix than Cu-phosphate phase. Incorporation of both mineral phases improved mechanical and water vapor barrier properties of the obtained mineralized films, in concentration dependent manner. When 5 mM of carbonate precursor was incorporated into agar matrix, mechanical resistance was enchanced for 44% and water vapor barrier property for 40%. The release of Cu (II) was higher in acidic conditions for both mineralized composites and remained in the range of specific release limits for this metal. In addition, both mineralized composite films exhibited distinctive antimicrobial activity against Gram-negative (Escherichia coli) and Gram-positive (Staphylococcus aureus) bacteria. Overall, the Cu-carbonate and Cu-phosphate mineralized agar films showed potential to be used for food packaging materials, agriculture or medical purposes.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - European Polymer Journal
T1  - Tailoring the physico-chemical and antimicrobial properties of agar-based films by in situ formation of Cu-mineral phase
EP  - 358
SP  - 352
VL  - 119
DO  - 10.1016/j.eurpolymj.2019.08.004
ER  - 

@article{
author = "Radovanović, Neda and Malagurski, Ivana and Lević, Steva and Gordić, Milan V. and Petrović, Jelena and Pavlović, Vladimir B. and Mitrić, Miodrag and Nešić, Aleksandra and Dimitrijević-Branković, Suzana",
year = "2019",
abstract = "New agar-based composite films with increasing Cu-carbonate and Cu-phosphate mineral phase content were prepared by in situ mineralization and solvent casting method. SEM and optical analysis revealed that Cu-carbonate phase had better compatibility with agar matrix than Cu-phosphate phase. Incorporation of both mineral phases improved mechanical and water vapor barrier properties of the obtained mineralized films, in concentration dependent manner. When 5 mM of carbonate precursor was incorporated into agar matrix, mechanical resistance was enchanced for 44% and water vapor barrier property for 40%. The release of Cu (II) was higher in acidic conditions for both mineralized composites and remained in the range of specific release limits for this metal. In addition, both mineralized composite films exhibited distinctive antimicrobial activity against Gram-negative (Escherichia coli) and Gram-positive (Staphylococcus aureus) bacteria. Overall, the Cu-carbonate and Cu-phosphate mineralized agar films showed potential to be used for food packaging materials, agriculture or medical purposes.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "European Polymer Journal",
title = "Tailoring the physico-chemical and antimicrobial properties of agar-based films by in situ formation of Cu-mineral phase",
pages = "358-352",
volume = "119",
doi = "10.1016/j.eurpolymj.2019.08.004"
}

Radovanović, N., Malagurski, I., Lević, S., Gordić, M. V., Petrović, J., Pavlović, V. B., Mitrić, M., Nešić, A.,& Dimitrijević-Branković, S.. (2019). Tailoring the physico-chemical and antimicrobial properties of agar-based films by in situ formation of Cu-mineral phase. in European Polymer Journal
Pergamon-Elsevier Science Ltd, Oxford., 119, 352-358.
https://doi.org/10.1016/j.eurpolymj.2019.08.004

Radovanović N, Malagurski I, Lević S, Gordić MV, Petrović J, Pavlović VB, Mitrić M, Nešić A, Dimitrijević-Branković S. Tailoring the physico-chemical and antimicrobial properties of agar-based films by in situ formation of Cu-mineral phase. in European Polymer Journal. 2019;119:352-358.
doi:10.1016/j.eurpolymj.2019.08.004 .

Radovanović, Neda, Malagurski, Ivana, Lević, Steva, Gordić, Milan V., Petrović, Jelena, Pavlović, Vladimir B., Mitrić, Miodrag, Nešić, Aleksandra, Dimitrijević-Branković, Suzana, "Tailoring the physico-chemical and antimicrobial properties of agar-based films by in situ formation of Cu-mineral phase" in European Polymer Journal, 119 (2019):352-358,
https://doi.org/10.1016/j.eurpolymj.2019.08.004 . .

TY  - JOUR
AU  - Radovanović, Neda
AU  - Malagurski, Ivana
AU  - Lević, Steva
AU  - Nešić, Aleksandra
AU  - Cabrera-Barjas, Gustavo
AU  - Kalušević, Ana
AU  - Nedović, Viktor
AU  - Pavlović, Vladimir
AU  - Dimitrijević-Branković, Suzana
PY  - 2019
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4048
AB  - Agar-based composites with different Zn-carbonate mineral phase content were prepared by in situ mineralization and the solvent casting method. The mineral phase within the composite films was identified as hydrozincite, Zn-5(CO3)(2)(OH)(6). The presence of the mineral phase improved, both mechanical and water vapor permeability properties of the obtained composite films, in a concentration-dependent manner. The release of zinc ions from composite films is in accepted levels (up to 2.5%), and sufficient to provide complete inhibition growth of S. Aureus. The results of this study suggest that agar/Zn-carbonate composites could be potentially used as affordable, eco-friendly and functional materials with tunable properties for food packaging, agriculture or biomedical application. In situ procedure offers possibilities for tailoring the physical-chemical properties of composite films, by varying the Zn-mineral phase load.
PB  - Elsevier, Amsterdam
T2  - Materials Letters
T1  - Influence of different concentrations of Zn-carbonate phase on physical-chemical properties of antimicrobial agar composite films
VL  - 255
DO  - 10.1016/j.matlet.2019.126572
ER  - 

@article{
author = "Radovanović, Neda and Malagurski, Ivana and Lević, Steva and Nešić, Aleksandra and Cabrera-Barjas, Gustavo and Kalušević, Ana and Nedović, Viktor and Pavlović, Vladimir and Dimitrijević-Branković, Suzana",
year = "2019",
abstract = "Agar-based composites with different Zn-carbonate mineral phase content were prepared by in situ mineralization and the solvent casting method. The mineral phase within the composite films was identified as hydrozincite, Zn-5(CO3)(2)(OH)(6). The presence of the mineral phase improved, both mechanical and water vapor permeability properties of the obtained composite films, in a concentration-dependent manner. The release of zinc ions from composite films is in accepted levels (up to 2.5%), and sufficient to provide complete inhibition growth of S. Aureus. The results of this study suggest that agar/Zn-carbonate composites could be potentially used as affordable, eco-friendly and functional materials with tunable properties for food packaging, agriculture or biomedical application. In situ procedure offers possibilities for tailoring the physical-chemical properties of composite films, by varying the Zn-mineral phase load.",
publisher = "Elsevier, Amsterdam",
journal = "Materials Letters",
title = "Influence of different concentrations of Zn-carbonate phase on physical-chemical properties of antimicrobial agar composite films",
volume = "255",
doi = "10.1016/j.matlet.2019.126572"
}

Radovanović, N., Malagurski, I., Lević, S., Nešić, A., Cabrera-Barjas, G., Kalušević, A., Nedović, V., Pavlović, V.,& Dimitrijević-Branković, S.. (2019). Influence of different concentrations of Zn-carbonate phase on physical-chemical properties of antimicrobial agar composite films. in Materials Letters
Elsevier, Amsterdam., 255.
https://doi.org/10.1016/j.matlet.2019.126572

Radovanović N, Malagurski I, Lević S, Nešić A, Cabrera-Barjas G, Kalušević A, Nedović V, Pavlović V, Dimitrijević-Branković S. Influence of different concentrations of Zn-carbonate phase on physical-chemical properties of antimicrobial agar composite films. in Materials Letters. 2019;255.
doi:10.1016/j.matlet.2019.126572 .

Radovanović, Neda, Malagurski, Ivana, Lević, Steva, Nešić, Aleksandra, Cabrera-Barjas, Gustavo, Kalušević, Ana, Nedović, Viktor, Pavlović, Vladimir, Dimitrijević-Branković, Suzana, "Influence of different concentrations of Zn-carbonate phase on physical-chemical properties of antimicrobial agar composite films" in Materials Letters, 255 (2019),
https://doi.org/10.1016/j.matlet.2019.126572 . .

TY  - CONF
AU  - Radovanović, Neda
AU  - Miljković, Miona
AU  - Davidović, Slađana
AU  - Malagurski, Ivana
AU  - Gordić, Milan
AU  - Nešić, Aleksandra
AU  - Dimitrijević-Branković, Suzana
PY  - 2018
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6286
C3  - Polychar 26 World Forum on Advanced Materials
T1  - ACTIVE AGAR MINERALIZED COMPOSITE FILMS INTENDED FOR FOOD PACKAGING
SP  - 94
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6286
ER  - 

@conference{
author = "Radovanović, Neda and Miljković, Miona and Davidović, Slađana and Malagurski, Ivana and Gordić, Milan and Nešić, Aleksandra and Dimitrijević-Branković, Suzana",
year = "2018",
journal = "Polychar 26 World Forum on Advanced Materials",
title = "ACTIVE AGAR MINERALIZED COMPOSITE FILMS INTENDED FOR FOOD PACKAGING",
pages = "94",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6286"
}

Radovanović, N., Miljković, M., Davidović, S., Malagurski, I., Gordić, M., Nešić, A.,& Dimitrijević-Branković, S.. (2018). ACTIVE AGAR MINERALIZED COMPOSITE FILMS INTENDED FOR FOOD PACKAGING. in Polychar 26 World Forum on Advanced Materials, 94.
https://hdl.handle.net/21.15107/rcub_technorep_6286

Radovanović N, Miljković M, Davidović S, Malagurski I, Gordić M, Nešić A, Dimitrijević-Branković S. ACTIVE AGAR MINERALIZED COMPOSITE FILMS INTENDED FOR FOOD PACKAGING. in Polychar 26 World Forum on Advanced Materials. 2018;:94.
https://hdl.handle.net/21.15107/rcub_technorep_6286 .

Radovanović, Neda, Miljković, Miona, Davidović, Slađana, Malagurski, Ivana, Gordić, Milan, Nešić, Aleksandra, Dimitrijević-Branković, Suzana, "ACTIVE AGAR MINERALIZED COMPOSITE FILMS INTENDED FOR FOOD PACKAGING" in Polychar 26 World Forum on Advanced Materials (2018):94,
https://hdl.handle.net/21.15107/rcub_technorep_6286 .

TY  - CONF
AU  - Davidović, Slađana
AU  - Miljković, Miona
AU  - Radovanović, Neda
AU  - Gordić, Milan
AU  - Nešić, Aleksandra
AU  - Dimitrijević, Suzana
PY  - 2018
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6204
AB  - Current trends in food biotechnology are focused on replacing synthetic polymers used for food packages by the natural ones. Biopolymers are made by sustainable processes in a cheap way. Moreover, they are nontoxic and can serve as a good alternative to traditional (petroleum) food packaging, due to great film-formation properties in a form of edible films and coatings for food products. Various polysaccharides, proteins, and lipids have been intensively investigated as edible materials that could improve the food quality, freshness, and provide food safety. However, natural polymers usually lack of antioxidative and/or antimicrobial properties. Therefore, many synthetic and natural additives can be incorporated into edible films. Among them, extracts of medicinal plants are giving more attention due to their safeness and proved health benefits.
The aim of this study was to obtain edible films based on dextran, with antioxidative and antimicrobial properties. Dextran obtained in our lab was used for preparing edible film with sorbitol as a plasticizer, while pulverized waste biomass obtained from the processing of medicinal herb yarrow (Achillea millefolium) was used as a source of polyphenols. It has previously been shown1 that dextran based films had excellent mechanical and water vapor barrier properties. In this work, spray dried ethanol extract of Achillea millefolium dust (AME) was incorporated into dextran films in concentrations 2, 3, and 4% (w/v). The composite films were analyzed regarding antioxidative, antimicrobial, and mechanical properties. All films showed high antioxidative activity (90% reduction of DPPH radical). However, to obtain high antimicrobial activity (higher than 50%), 4% of AME was required. Composite films showed antimicrobial activity against three Gram-positive bacteria (Staphylococcus aureus, Listeria monocytogenes and Enterococcus faecalis and one Gram-negative bacterium (Pseudomonas aeruginosa). Incorporation of AME into dextran film showed positive effect on tensile strength and negative effect on elongation. Composite film with the best mechanical properties (tensile strength 3.5 MPa and elongation at break 37%) was the film containing 4% AME.
C3  - Polychar 26 World Forum on Advanced Materials
T1  - ANTIOXIDANT AND ANTIMICROBIAL EDIBLE FILMS BASED ON DEXTRAN CONTAINING POLYPHENOLS FROM YARROW EXTRACT
SP  - 72
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6204
ER  - 

@conference{
author = "Davidović, Slađana and Miljković, Miona and Radovanović, Neda and Gordić, Milan and Nešić, Aleksandra and Dimitrijević, Suzana",
year = "2018",
abstract = "Current trends in food biotechnology are focused on replacing synthetic polymers used for food packages by the natural ones. Biopolymers are made by sustainable processes in a cheap way. Moreover, they are nontoxic and can serve as a good alternative to traditional (petroleum) food packaging, due to great film-formation properties in a form of edible films and coatings for food products. Various polysaccharides, proteins, and lipids have been intensively investigated as edible materials that could improve the food quality, freshness, and provide food safety. However, natural polymers usually lack of antioxidative and/or antimicrobial properties. Therefore, many synthetic and natural additives can be incorporated into edible films. Among them, extracts of medicinal plants are giving more attention due to their safeness and proved health benefits.
The aim of this study was to obtain edible films based on dextran, with antioxidative and antimicrobial properties. Dextran obtained in our lab was used for preparing edible film with sorbitol as a plasticizer, while pulverized waste biomass obtained from the processing of medicinal herb yarrow (Achillea millefolium) was used as a source of polyphenols. It has previously been shown1 that dextran based films had excellent mechanical and water vapor barrier properties. In this work, spray dried ethanol extract of Achillea millefolium dust (AME) was incorporated into dextran films in concentrations 2, 3, and 4% (w/v). The composite films were analyzed regarding antioxidative, antimicrobial, and mechanical properties. All films showed high antioxidative activity (90% reduction of DPPH radical). However, to obtain high antimicrobial activity (higher than 50%), 4% of AME was required. Composite films showed antimicrobial activity against three Gram-positive bacteria (Staphylococcus aureus, Listeria monocytogenes and Enterococcus faecalis and one Gram-negative bacterium (Pseudomonas aeruginosa). Incorporation of AME into dextran film showed positive effect on tensile strength and negative effect on elongation. Composite film with the best mechanical properties (tensile strength 3.5 MPa and elongation at break 37%) was the film containing 4% AME.",
journal = "Polychar 26 World Forum on Advanced Materials",
title = "ANTIOXIDANT AND ANTIMICROBIAL EDIBLE FILMS BASED ON DEXTRAN CONTAINING POLYPHENOLS FROM YARROW EXTRACT",
pages = "72",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6204"
}

Davidović, S., Miljković, M., Radovanović, N., Gordić, M., Nešić, A.,& Dimitrijević, S.. (2018). ANTIOXIDANT AND ANTIMICROBIAL EDIBLE FILMS BASED ON DEXTRAN CONTAINING POLYPHENOLS FROM YARROW EXTRACT. in Polychar 26 World Forum on Advanced Materials, 72.
https://hdl.handle.net/21.15107/rcub_technorep_6204

Davidović S, Miljković M, Radovanović N, Gordić M, Nešić A, Dimitrijević S. ANTIOXIDANT AND ANTIMICROBIAL EDIBLE FILMS BASED ON DEXTRAN CONTAINING POLYPHENOLS FROM YARROW EXTRACT. in Polychar 26 World Forum on Advanced Materials. 2018;:72.
https://hdl.handle.net/21.15107/rcub_technorep_6204 .

Davidović, Slađana, Miljković, Miona, Radovanović, Neda, Gordić, Milan, Nešić, Aleksandra, Dimitrijević, Suzana, "ANTIOXIDANT AND ANTIMICROBIAL EDIBLE FILMS BASED ON DEXTRAN CONTAINING POLYPHENOLS FROM YARROW EXTRACT" in Polychar 26 World Forum on Advanced Materials (2018):72,
https://hdl.handle.net/21.15107/rcub_technorep_6204 .

TY  - CONF
AU  - Miljković, Miona
AU  - Davidović, Slađana
AU  - Radovanović, Neda
AU  - Gordić, Milan
AU  - Carević, Milica
AU  - Nešić, Aleksandra
AU  - Dimitrijević, Suzana
PY  - 2018
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6239
AB  - Dextransucrase (DS), the extracellular enzyme is of immense industrial importance, due to ability to produce dextran and oligosaccharides (OS). Worldwide interest in OS has been increasing, since they have been accorded the prebiotic status. However, the industrial application of DS for OS synthesis is limited, due to low yield of enzyme production and its low catalytic activity. Hence, there is a great interest for development of new technologies that can provide improved performance of biocatalyst. Enzyme immobilization technology is considered to be a crucial step for cheaper and more efficient usage of DS. Entrapment is one of the widely investigated immobilization methods, where enzymes are enclosed or confined within the polymer matrix without altering their native structure, developing bioreactors for commercial applications. Different matrices such as polyacryl-amide gel, alginate beads and agar–agar have been used for the entrapment of different enzymes and among them agar–agar is a biocompatible, non-toxic and strong solidifying agent for immobilization of various enzymes.
In this work, the entrapment of DS was initiated by different quantity (1:9, 1:4 and 1:1) of dialyzed enzyme into agar- agar solution. Agar solution was prepared in distilled water by vigorous shaking at 100°C, autoclaved and was allowed to cool to 40–45°C. Afterwards, enzyme was incorporated and mixed thoroughly. This mixture was immediately poured into a clean Petri plate and left to solidify at room temperature. Polymer films with and without immobilized DS were analyzed in terms of enzyme activity and reusability and mechanical properties (tensile strength, elongation at break and elastic modulus). In order to remove un-entrapped enzyme the films were washed with double deionized water and sodium acetate buffer (pH 5.4) three times prior to enzymatic assay. For quantitative analysis of samples for OS production a Dionex Ultimate 3000 HPLC system was used. 
Results showed that maximum immobilization yield (74.11%) was achieved by use of 2 % agar and (1:9) enzyme: agar ratio. HPLC analysis confirmed the similar trend of OS formation in immobilized samples compared to free enzyme. The lowest tested fraction of enzyme immobilized into polymer matrix (1:9) improved tensile strength of films in comparison with control film, whereas higher concentration of enzyme led to decrease in mechanical resistance of films. Scanning electron microscopy of agar films before and after DS entrapment revealed significant morphological change on the matrix surface. Considering the economic feasibility, the entrapped DS indicated imperative recycling efficiency up to six reaction cycles. The results of this study revealed that an easily available and inexpensive matrix could be successfully employed for DS immobilization and OS production.
C3  - PolyChar 26 World Forum on Advanced Materials
T1  - DEXTRANSUCRASE ENTRAPMENT AS AN EFFICIENT ALTERNATIVE FOR INCREASED RECYCLING EFFICIENCY OF FREE ENZYME WITHIN AGAR-AGAR FILM MATRIX
SP  - 88
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6239
ER  - 

@conference{
author = "Miljković, Miona and Davidović, Slađana and Radovanović, Neda and Gordić, Milan and Carević, Milica and Nešić, Aleksandra and Dimitrijević, Suzana",
year = "2018",
abstract = "Dextransucrase (DS), the extracellular enzyme is of immense industrial importance, due to ability to produce dextran and oligosaccharides (OS). Worldwide interest in OS has been increasing, since they have been accorded the prebiotic status. However, the industrial application of DS for OS synthesis is limited, due to low yield of enzyme production and its low catalytic activity. Hence, there is a great interest for development of new technologies that can provide improved performance of biocatalyst. Enzyme immobilization technology is considered to be a crucial step for cheaper and more efficient usage of DS. Entrapment is one of the widely investigated immobilization methods, where enzymes are enclosed or confined within the polymer matrix without altering their native structure, developing bioreactors for commercial applications. Different matrices such as polyacryl-amide gel, alginate beads and agar–agar have been used for the entrapment of different enzymes and among them agar–agar is a biocompatible, non-toxic and strong solidifying agent for immobilization of various enzymes.
In this work, the entrapment of DS was initiated by different quantity (1:9, 1:4 and 1:1) of dialyzed enzyme into agar- agar solution. Agar solution was prepared in distilled water by vigorous shaking at 100°C, autoclaved and was allowed to cool to 40–45°C. Afterwards, enzyme was incorporated and mixed thoroughly. This mixture was immediately poured into a clean Petri plate and left to solidify at room temperature. Polymer films with and without immobilized DS were analyzed in terms of enzyme activity and reusability and mechanical properties (tensile strength, elongation at break and elastic modulus). In order to remove un-entrapped enzyme the films were washed with double deionized water and sodium acetate buffer (pH 5.4) three times prior to enzymatic assay. For quantitative analysis of samples for OS production a Dionex Ultimate 3000 HPLC system was used. 
Results showed that maximum immobilization yield (74.11%) was achieved by use of 2 % agar and (1:9) enzyme: agar ratio. HPLC analysis confirmed the similar trend of OS formation in immobilized samples compared to free enzyme. The lowest tested fraction of enzyme immobilized into polymer matrix (1:9) improved tensile strength of films in comparison with control film, whereas higher concentration of enzyme led to decrease in mechanical resistance of films. Scanning electron microscopy of agar films before and after DS entrapment revealed significant morphological change on the matrix surface. Considering the economic feasibility, the entrapped DS indicated imperative recycling efficiency up to six reaction cycles. The results of this study revealed that an easily available and inexpensive matrix could be successfully employed for DS immobilization and OS production.",
journal = "PolyChar 26 World Forum on Advanced Materials",
title = "DEXTRANSUCRASE ENTRAPMENT AS AN EFFICIENT ALTERNATIVE FOR INCREASED RECYCLING EFFICIENCY OF FREE ENZYME WITHIN AGAR-AGAR FILM MATRIX",
pages = "88",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6239"
}

Miljković, M., Davidović, S., Radovanović, N., Gordić, M., Carević, M., Nešić, A.,& Dimitrijević, S.. (2018). DEXTRANSUCRASE ENTRAPMENT AS AN EFFICIENT ALTERNATIVE FOR INCREASED RECYCLING EFFICIENCY OF FREE ENZYME WITHIN AGAR-AGAR FILM MATRIX. in PolyChar 26 World Forum on Advanced Materials, 88.
https://hdl.handle.net/21.15107/rcub_technorep_6239

Miljković M, Davidović S, Radovanović N, Gordić M, Carević M, Nešić A, Dimitrijević S. DEXTRANSUCRASE ENTRAPMENT AS AN EFFICIENT ALTERNATIVE FOR INCREASED RECYCLING EFFICIENCY OF FREE ENZYME WITHIN AGAR-AGAR FILM MATRIX. in PolyChar 26 World Forum on Advanced Materials. 2018;:88.
https://hdl.handle.net/21.15107/rcub_technorep_6239 .

Miljković, Miona, Davidović, Slađana, Radovanović, Neda, Gordić, Milan, Carević, Milica, Nešić, Aleksandra, Dimitrijević, Suzana, "DEXTRANSUCRASE ENTRAPMENT AS AN EFFICIENT ALTERNATIVE FOR INCREASED RECYCLING EFFICIENCY OF FREE ENZYME WITHIN AGAR-AGAR FILM MATRIX" in PolyChar 26 World Forum on Advanced Materials (2018):88,
https://hdl.handle.net/21.15107/rcub_technorep_6239 .

TY  - JOUR
AU  - Radovanović, Neda
AU  - Davidović, Slađana
AU  - Miljković, Miona
AU  - Pavlović, Marija
AU  - Buntić, Aneta
AU  - Lazić, Vesna
AU  - Mihajlovski, Katarina
PY  - 2018
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3815
AB  - Amylases are industrially important enzymes which could convert starch to glucose, maltose and oligosaccharides. A bacterial strain designated as Paenibacillus chitinolyticus CKS1which was isolated from the soil of the coniferous forest, produced β-amylases using different commercial and waste substrates. Maximum β-amylases activity of 0.820 U/mL was obtained using a sugar alcohol-isomaltidex (0.5% w/v), as a substrate for microorganism growth and enzyme production. After 48 h of fermentation in a medium that contained starch (0.5%, w/v) and 0.05% v/v of ethanol, CKS1 produced β-amylase with the activity of 0.518 U/mL. The latest trends in enzyme production include utilisation of various waste products, mainly of agroindustrial origin, as a substrate for microorganisms growth. The strain CKS1 was also able to grow and produce β-amylases by using plant waste material. The plant waste substrate (PWS) contained plant biomass that is left after the ethanol extraction of various medicinal herbs (marigold and chamomile flowers, artichoke leaf, lemon balm leaf, nettle leaf, thyme leaf, yarrow shoot, yellow gentian shoot, primrose shoot, valerian shoot and chestnut and hawthorn seeds).This mixture of dried plant biomass is dissposed as such as a waste. In a medium with 0.1% (w/v) of PWS, CKS1 produced β-amylases with a maximum activity of 0.569 U/mL. The results show the potential of utilising waste plant biomass, left after ethanol extraction of medicinal herbs, in production of amylases. The application of microorganisms in β-amylase production using waste substrate is economically and environmentally accepted.
AB  - Amilaze predstavljaju grupu industrijski veoma važnih enzima koji hidrolizuju skrob do glukoze, maltoze i različitih oligosaharida. Bakterijski izolat Paenibacillus chitinolyticus CKS1, izolovan iz zemljišta četinarske šume, pokazao je sposobnost proizvodnje β-amilaza tokom svog rasta na različitim komercijalnim ali i na otpadnim supstratima. Maksimalna aktivnosti β-amilaza koja je iznosila 0,820 U/mL postignuta je korišćenjem izomaltideksa, šećernog alkohola, u koncentraciji od 0,5% (w/v), kao supstrata za rast mikroorganizma i proizvodnju enzima. U podlozi sa skrobom (0,5% w/v) i sa dodatkom 0,05% (v/v) etanola, nakon 48h fermentacije, maksimum aktivnosti β-amilaza iznosila je 0,518 U/mL. Najnoviji trendovi u proizvodnji enzima odnose se na korišćenje različitih otpadnih sirovina agroindustrijskog porekla kao supstrata za rast mikroorganizma. Soj CKS1 je pokazao mogućnost korišćenja otpadnog biljnog materijala kao supstrata za rast i proizvodnju enzima amilaza. Otpadni biljni supstrat (PWS), činila je biljna masa zaostala nakon etanolne ekstrakcije različitog lekovitog bilja (cveta nevena i kamilice, lista matičnjaka, artičoke, koprive, timijana, nadzemnog dela hajdučke trave, lincure, jagorčevine i valerijane i semena divljeg kestena i gloga). Ova biljna biomasa, iz koje su ekstrahovane bioaktivne materije, meša se i odlaže kao takva u vidu otpada. Nakon rasta u podlozi sa 1,0 % (w/v) PWS, soj CKS1 proizveo je β-amilaze sa aktivnošću od 0,569 U/mL. Ovi reziltati ukazuju na mogućnost iskorišćenja otpadne biljne biomase, zaostale nakon alkoholne ekstrakcije lekovitog bilja, u procesima ekonomičnije proizvodnje amilaza. Korišćenje otpadne sirovine u mikrobnim procesima proizvodnje enzima je i ekološki mnogo prihvatljivije usled uticaja na smanjene skladištenja otpada a samim tim i sveukupnog zagađenje životne sredine.
PB  - Nacionalno društvo za procesnu tehniku i energetiku u poljoprivredi, Novi Sad
T2  - Journal on Processing and Energy in Agriculture
T1  - β-amylase production by a novel strain Paenibacillus chitinolyticus CKS1 using commercial and waste substrates
T1  - Proizvodnja β-amilaza pomoću novog bakterijskog soja Paenibacillus chitinolyticus CKS1 na komercijalnim i otpadnim supstratima
EP  - 22
IS  - 1
SP  - 18
VL  - 22
DO  - 10.5937/JPEA1801018R
ER  - 

@article{
author = "Radovanović, Neda and Davidović, Slađana and Miljković, Miona and Pavlović, Marija and Buntić, Aneta and Lazić, Vesna and Mihajlovski, Katarina",
year = "2018",
abstract = "Amylases are industrially important enzymes which could convert starch to glucose, maltose and oligosaccharides. A bacterial strain designated as Paenibacillus chitinolyticus CKS1which was isolated from the soil of the coniferous forest, produced β-amylases using different commercial and waste substrates. Maximum β-amylases activity of 0.820 U/mL was obtained using a sugar alcohol-isomaltidex (0.5% w/v), as a substrate for microorganism growth and enzyme production. After 48 h of fermentation in a medium that contained starch (0.5%, w/v) and 0.05% v/v of ethanol, CKS1 produced β-amylase with the activity of 0.518 U/mL. The latest trends in enzyme production include utilisation of various waste products, mainly of agroindustrial origin, as a substrate for microorganisms growth. The strain CKS1 was also able to grow and produce β-amylases by using plant waste material. The plant waste substrate (PWS) contained plant biomass that is left after the ethanol extraction of various medicinal herbs (marigold and chamomile flowers, artichoke leaf, lemon balm leaf, nettle leaf, thyme leaf, yarrow shoot, yellow gentian shoot, primrose shoot, valerian shoot and chestnut and hawthorn seeds).This mixture of dried plant biomass is dissposed as such as a waste. In a medium with 0.1% (w/v) of PWS, CKS1 produced β-amylases with a maximum activity of 0.569 U/mL. The results show the potential of utilising waste plant biomass, left after ethanol extraction of medicinal herbs, in production of amylases. The application of microorganisms in β-amylase production using waste substrate is economically and environmentally accepted., Amilaze predstavljaju grupu industrijski veoma važnih enzima koji hidrolizuju skrob do glukoze, maltoze i različitih oligosaharida. Bakterijski izolat Paenibacillus chitinolyticus CKS1, izolovan iz zemljišta četinarske šume, pokazao je sposobnost proizvodnje β-amilaza tokom svog rasta na različitim komercijalnim ali i na otpadnim supstratima. Maksimalna aktivnosti β-amilaza koja je iznosila 0,820 U/mL postignuta je korišćenjem izomaltideksa, šećernog alkohola, u koncentraciji od 0,5% (w/v), kao supstrata za rast mikroorganizma i proizvodnju enzima. U podlozi sa skrobom (0,5% w/v) i sa dodatkom 0,05% (v/v) etanola, nakon 48h fermentacije, maksimum aktivnosti β-amilaza iznosila je 0,518 U/mL. Najnoviji trendovi u proizvodnji enzima odnose se na korišćenje različitih otpadnih sirovina agroindustrijskog porekla kao supstrata za rast mikroorganizma. Soj CKS1 je pokazao mogućnost korišćenja otpadnog biljnog materijala kao supstrata za rast i proizvodnju enzima amilaza. Otpadni biljni supstrat (PWS), činila je biljna masa zaostala nakon etanolne ekstrakcije različitog lekovitog bilja (cveta nevena i kamilice, lista matičnjaka, artičoke, koprive, timijana, nadzemnog dela hajdučke trave, lincure, jagorčevine i valerijane i semena divljeg kestena i gloga). Ova biljna biomasa, iz koje su ekstrahovane bioaktivne materije, meša se i odlaže kao takva u vidu otpada. Nakon rasta u podlozi sa 1,0 % (w/v) PWS, soj CKS1 proizveo je β-amilaze sa aktivnošću od 0,569 U/mL. Ovi reziltati ukazuju na mogućnost iskorišćenja otpadne biljne biomase, zaostale nakon alkoholne ekstrakcije lekovitog bilja, u procesima ekonomičnije proizvodnje amilaza. Korišćenje otpadne sirovine u mikrobnim procesima proizvodnje enzima je i ekološki mnogo prihvatljivije usled uticaja na smanjene skladištenja otpada a samim tim i sveukupnog zagađenje životne sredine.",
publisher = "Nacionalno društvo za procesnu tehniku i energetiku u poljoprivredi, Novi Sad",
journal = "Journal on Processing and Energy in Agriculture",
title = "β-amylase production by a novel strain Paenibacillus chitinolyticus CKS1 using commercial and waste substrates, Proizvodnja β-amilaza pomoću novog bakterijskog soja Paenibacillus chitinolyticus CKS1 na komercijalnim i otpadnim supstratima",
pages = "22-18",
number = "1",
volume = "22",
doi = "10.5937/JPEA1801018R"
}

Radovanović, N., Davidović, S., Miljković, M., Pavlović, M., Buntić, A., Lazić, V.,& Mihajlovski, K.. (2018). β-amylase production by a novel strain Paenibacillus chitinolyticus CKS1 using commercial and waste substrates. in Journal on Processing and Energy in Agriculture
Nacionalno društvo za procesnu tehniku i energetiku u poljoprivredi, Novi Sad., 22(1), 18-22.
https://doi.org/10.5937/JPEA1801018R

Radovanović N, Davidović S, Miljković M, Pavlović M, Buntić A, Lazić V, Mihajlovski K. β-amylase production by a novel strain Paenibacillus chitinolyticus CKS1 using commercial and waste substrates. in Journal on Processing and Energy in Agriculture. 2018;22(1):18-22.
doi:10.5937/JPEA1801018R .

Radovanović, Neda, Davidović, Slađana, Miljković, Miona, Pavlović, Marija, Buntić, Aneta, Lazić, Vesna, Mihajlovski, Katarina, "β-amylase production by a novel strain Paenibacillus chitinolyticus CKS1 using commercial and waste substrates" in Journal on Processing and Energy in Agriculture, 22, no. 1 (2018):18-22,
https://doi.org/10.5937/JPEA1801018R . .

TY  - JOUR
AU  - Radovanović, Neda
AU  - Milutinović, Milica
AU  - Mihajlovski, Katarina
AU  - Jović, Jelena M.
AU  - Nastasijević, Branislav J.
AU  - Rajilić-Stojanović, Mirjana
AU  - Dimitrijević-Branković, Suzana
PY  - 2018
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3948
AB  - In the current study, the biocontrol potential of a novel strain Bacillus sp. PPM3 isolated from marine sediment from the Red Sea in Hurghada, Egypt is recognized. This novel strain was selected out of 32 isolates based on its ability to suppress the growth of four plant pathogenic fungi: Aspergillus flavus, Fusariurn graminearum, Mucor sp. and Alternaria sp. The new marine strain was identified and characterized by phenotypic and molecular approaches. The culture filtrate of Bacillus sp. PPM3 suppressed the growth and spore germination of all tested fungi in vitro with the highest value of inhibition reported for Mucor sp. (97.5%). The antifungal effect of the culture filtrate from the strain PPM3 was due to production of highly stable secondary metabolites resistant to extreme pH, temperature and enzymatic treatments. A PCR analysis confirmed the expression of genes involved in the synthesis of antifungal lipopeptides: iturin, bacillomycin D, mycosubtilin and surfactin. In a greenhouse experiment strain PPM3 effectively reduced disease incidence of F. graminearum in maize plants and displayed additional plant growth stimulating effect. The results show that novel marine strain PPM3 could have a potential in commercial application as biocontrol agent for treatment of various plant diseases caused by soil-borne and postharvest pathogenic fungi.
PB  - Academic Press Ltd- Elsevier Science Ltd, London
T2  - Microbial Pathogenesis
T1  - Biocontrol and plant stimulating potential of novel strain Bacillus sp. PPM3 isolated from marine sediment
EP  - 78
SP  - 71
VL  - 120
DO  - 10.1016/j.micpath.2018.04.056
ER  - 

@article{
author = "Radovanović, Neda and Milutinović, Milica and Mihajlovski, Katarina and Jović, Jelena M. and Nastasijević, Branislav J. and Rajilić-Stojanović, Mirjana and Dimitrijević-Branković, Suzana",
year = "2018",
abstract = "In the current study, the biocontrol potential of a novel strain Bacillus sp. PPM3 isolated from marine sediment from the Red Sea in Hurghada, Egypt is recognized. This novel strain was selected out of 32 isolates based on its ability to suppress the growth of four plant pathogenic fungi: Aspergillus flavus, Fusariurn graminearum, Mucor sp. and Alternaria sp. The new marine strain was identified and characterized by phenotypic and molecular approaches. The culture filtrate of Bacillus sp. PPM3 suppressed the growth and spore germination of all tested fungi in vitro with the highest value of inhibition reported for Mucor sp. (97.5%). The antifungal effect of the culture filtrate from the strain PPM3 was due to production of highly stable secondary metabolites resistant to extreme pH, temperature and enzymatic treatments. A PCR analysis confirmed the expression of genes involved in the synthesis of antifungal lipopeptides: iturin, bacillomycin D, mycosubtilin and surfactin. In a greenhouse experiment strain PPM3 effectively reduced disease incidence of F. graminearum in maize plants and displayed additional plant growth stimulating effect. The results show that novel marine strain PPM3 could have a potential in commercial application as biocontrol agent for treatment of various plant diseases caused by soil-borne and postharvest pathogenic fungi.",
publisher = "Academic Press Ltd- Elsevier Science Ltd, London",
journal = "Microbial Pathogenesis",
title = "Biocontrol and plant stimulating potential of novel strain Bacillus sp. PPM3 isolated from marine sediment",
pages = "78-71",
volume = "120",
doi = "10.1016/j.micpath.2018.04.056"
}

Radovanović, N., Milutinović, M., Mihajlovski, K., Jović, J. M., Nastasijević, B. J., Rajilić-Stojanović, M.,& Dimitrijević-Branković, S.. (2018). Biocontrol and plant stimulating potential of novel strain Bacillus sp. PPM3 isolated from marine sediment. in Microbial Pathogenesis
Academic Press Ltd- Elsevier Science Ltd, London., 120, 71-78.
https://doi.org/10.1016/j.micpath.2018.04.056

Radovanović N, Milutinović M, Mihajlovski K, Jović JM, Nastasijević BJ, Rajilić-Stojanović M, Dimitrijević-Branković S. Biocontrol and plant stimulating potential of novel strain Bacillus sp. PPM3 isolated from marine sediment. in Microbial Pathogenesis. 2018;120:71-78.
doi:10.1016/j.micpath.2018.04.056 .

Radovanović, Neda, Milutinović, Milica, Mihajlovski, Katarina, Jović, Jelena M., Nastasijević, Branislav J., Rajilić-Stojanović, Mirjana, Dimitrijević-Branković, Suzana, "Biocontrol and plant stimulating potential of novel strain Bacillus sp. PPM3 isolated from marine sediment" in Microbial Pathogenesis, 120 (2018):71-78,
https://doi.org/10.1016/j.micpath.2018.04.056 . .

TY  - JOUR
AU  - Jović, Jelena
AU  - Buntić, Aneta
AU  - Radovanović, Neda
AU  - Petrović, Bojan
AU  - Mojović, Ljiljana
PY  - 2018
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3932
AB  - The aim of this research is to isolate and identify fungi with high lignin-degrading abilities that are autochthonous to southern Serbian region. Two novel fungal isolates identified as Trametes hirsuta F13 and Stereum gausapatum F28 were selected to assess their ligninolytic enzyme activities and the efficiency of lignin removal from beech wood sawdust. Obtained results show that both isolates are good sources of industrially valuable enzymes with a potential for application in various biotechnological and industrial processes. Both isolates showed laccase, manganese-dependent peroxidase, and versatile peroxidase activities, while only S. gausapatum F28 had lignin peroxidase activity. This is the first record of the ability of S. gausapatum species to produce lignin peroxidase. T. hirsuta F13 showed higher laccase activity than S. gausapatum F28, while S. gausapatum F28 had higher manganese peroxidase activity. Also, T hirsuta F13 exhibited much higher laccase activity under submerged cultivation conditions than solid-state cultivation conditions, which is rare for fungi. This is important for industrial processes since the submerged fermentation is a dominant technique in industry. The test of the efficiency of lignin removal showed that both isolates are efficient lignin decomposers. After five weeks of incubation on beech wood sawdust, the total lignin losses were 33.84 % with T. hirsuta F13 and 28.8 % with S. gausapatum F28.
PB  - Faculty Food Technology Biotechnology, Zagreb
T2  - Food Technology and Biotechnology
T1  - Lignin-Degrading Abilities of Novel Autochthonous Fungal Isolates Trametes hirsuta F13 and Stereum gausapatum F28
EP  - 365
IS  - 3
SP  - 354
VL  - 56
DO  - 10.17113/ftb.56.03.18.5348
ER  - 

@article{
author = "Jović, Jelena and Buntić, Aneta and Radovanović, Neda and Petrović, Bojan and Mojović, Ljiljana",
year = "2018",
abstract = "The aim of this research is to isolate and identify fungi with high lignin-degrading abilities that are autochthonous to southern Serbian region. Two novel fungal isolates identified as Trametes hirsuta F13 and Stereum gausapatum F28 were selected to assess their ligninolytic enzyme activities and the efficiency of lignin removal from beech wood sawdust. Obtained results show that both isolates are good sources of industrially valuable enzymes with a potential for application in various biotechnological and industrial processes. Both isolates showed laccase, manganese-dependent peroxidase, and versatile peroxidase activities, while only S. gausapatum F28 had lignin peroxidase activity. This is the first record of the ability of S. gausapatum species to produce lignin peroxidase. T. hirsuta F13 showed higher laccase activity than S. gausapatum F28, while S. gausapatum F28 had higher manganese peroxidase activity. Also, T hirsuta F13 exhibited much higher laccase activity under submerged cultivation conditions than solid-state cultivation conditions, which is rare for fungi. This is important for industrial processes since the submerged fermentation is a dominant technique in industry. The test of the efficiency of lignin removal showed that both isolates are efficient lignin decomposers. After five weeks of incubation on beech wood sawdust, the total lignin losses were 33.84 % with T. hirsuta F13 and 28.8 % with S. gausapatum F28.",
publisher = "Faculty Food Technology Biotechnology, Zagreb",
journal = "Food Technology and Biotechnology",
title = "Lignin-Degrading Abilities of Novel Autochthonous Fungal Isolates Trametes hirsuta F13 and Stereum gausapatum F28",
pages = "365-354",
number = "3",
volume = "56",
doi = "10.17113/ftb.56.03.18.5348"
}

Jović, J., Buntić, A., Radovanović, N., Petrović, B.,& Mojović, L.. (2018). Lignin-Degrading Abilities of Novel Autochthonous Fungal Isolates Trametes hirsuta F13 and Stereum gausapatum F28. in Food Technology and Biotechnology
Faculty Food Technology Biotechnology, Zagreb., 56(3), 354-365.
https://doi.org/10.17113/ftb.56.03.18.5348

Jović J, Buntić A, Radovanović N, Petrović B, Mojović L. Lignin-Degrading Abilities of Novel Autochthonous Fungal Isolates Trametes hirsuta F13 and Stereum gausapatum F28. in Food Technology and Biotechnology. 2018;56(3):354-365.
doi:10.17113/ftb.56.03.18.5348 .

Jović, Jelena, Buntić, Aneta, Radovanović, Neda, Petrović, Bojan, Mojović, Ljiljana, "Lignin-Degrading Abilities of Novel Autochthonous Fungal Isolates Trametes hirsuta F13 and Stereum gausapatum F28" in Food Technology and Biotechnology, 56, no. 3 (2018):354-365,
https://doi.org/10.17113/ftb.56.03.18.5348 . .

TY  - JOUR
AU  - Davidović, Slađana
AU  - Miljković, Miona
AU  - Radovanović, Neda
AU  - Dimitrijević, Suzana
AU  - Nešić, Aleksandra
PY  - 2017
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6183
AB  - In this work, antimicrobial agar based nanocomposite films containing silver nanoparticles were prepared. For the synthesis, a masterbatch of in situ stabilized silver nanoparticles produced in agar solution was used. The incorporation of silver nanoparticles improved the mechanical and water vapor barrier resistance of films, in comparison to the neat agar film without silver. Agar/Ag films showed high antimicrobial activity against Staphylococcus aureus, Escherichia coli and Candida albicans. The migration of silver nanoparticles from the agar films, assessed by food contact tests, was less than 0.05 mg/g which is under the legal limits. These results indicated that the agar films incorporated with silver nanoparticles have potential to be used as packaging material.
T2  - Journal of International Scientific Publications, Agriculture & Food
T1  - PHYSICO-CHEMICAL PROPERTIES OF AGAR/SILVER NANOCOMPOSITE FILMS INTENDED FOR FOOD PACKAGING APPLICATION
EP  - 146
SP  - 139
VL  - 5
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6183
ER  - 

@article{
author = "Davidović, Slađana and Miljković, Miona and Radovanović, Neda and Dimitrijević, Suzana and Nešić, Aleksandra",
year = "2017",
abstract = "In this work, antimicrobial agar based nanocomposite films containing silver nanoparticles were prepared. For the synthesis, a masterbatch of in situ stabilized silver nanoparticles produced in agar solution was used. The incorporation of silver nanoparticles improved the mechanical and water vapor barrier resistance of films, in comparison to the neat agar film without silver. Agar/Ag films showed high antimicrobial activity against Staphylococcus aureus, Escherichia coli and Candida albicans. The migration of silver nanoparticles from the agar films, assessed by food contact tests, was less than 0.05 mg/g which is under the legal limits. These results indicated that the agar films incorporated with silver nanoparticles have potential to be used as packaging material.",
journal = "Journal of International Scientific Publications, Agriculture & Food",
title = "PHYSICO-CHEMICAL PROPERTIES OF AGAR/SILVER NANOCOMPOSITE FILMS INTENDED FOR FOOD PACKAGING APPLICATION",
pages = "146-139",
volume = "5",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6183"
}

Davidović, S., Miljković, M., Radovanović, N., Dimitrijević, S.,& Nešić, A.. (2017). PHYSICO-CHEMICAL PROPERTIES OF AGAR/SILVER NANOCOMPOSITE FILMS INTENDED FOR FOOD PACKAGING APPLICATION. in Journal of International Scientific Publications, Agriculture & Food, 5, 139-146.
https://hdl.handle.net/21.15107/rcub_technorep_6183

Davidović S, Miljković M, Radovanović N, Dimitrijević S, Nešić A. PHYSICO-CHEMICAL PROPERTIES OF AGAR/SILVER NANOCOMPOSITE FILMS INTENDED FOR FOOD PACKAGING APPLICATION. in Journal of International Scientific Publications, Agriculture & Food. 2017;5:139-146.
https://hdl.handle.net/21.15107/rcub_technorep_6183 .

Davidović, Slađana, Miljković, Miona, Radovanović, Neda, Dimitrijević, Suzana, Nešić, Aleksandra, "PHYSICO-CHEMICAL PROPERTIES OF AGAR/SILVER NANOCOMPOSITE FILMS INTENDED FOR FOOD PACKAGING APPLICATION" in Journal of International Scientific Publications, Agriculture & Food, 5 (2017):139-146,
https://hdl.handle.net/21.15107/rcub_technorep_6183 .

TY  - JOUR
AU  - Miljković, Miona
AU  - Davidović, Slađana
AU  - Davidović, Slađana
AU  - Radovanović, Neda
AU  - Dimitrijević, Suzana
PY  - 2017
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6247
AB  - In this work, the pectin/oregano oil nanoemulsion coatings were investigated, in order to prolong the shelf-life of cheese. The water vapor barrier, mechanical and antimicrobial activity of prepared nanoemulsion coatings were examined. In addition, the textural properties of uncoated and pectin/oregano oil coated cheeses were followed for 2 weeks. The results showed that obtained coatings possessed high antimicrobial activity toward E. coli, S. aureus and C. albicans and satisfied mechanical and water vapor barrier resistance. The coatings were also effective on reducing losses of weight and firmness of cheeses. Overall, antimicrobial nanoemulsion/edible films from renewable sources were successfully produced and showed high potential to be applicable in food industry to prolong the shelf-life of cheeses.
PB  - Sciences Invest
T2  - Journal of International Scientific Publications Agriculture & Food
T1  - The use of nanoemulsion-based edible coatings to prolong the shelflife of cheese
EP  - 138
SP  - 131
VL  - 5
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6247
ER  - 

@article{
author = "Miljković, Miona and Davidović, Slađana and Davidović, Slađana and Radovanović, Neda and Dimitrijević, Suzana",
year = "2017",
abstract = "In this work, the pectin/oregano oil nanoemulsion coatings were investigated, in order to prolong the shelf-life of cheese. The water vapor barrier, mechanical and antimicrobial activity of prepared nanoemulsion coatings were examined. In addition, the textural properties of uncoated and pectin/oregano oil coated cheeses were followed for 2 weeks. The results showed that obtained coatings possessed high antimicrobial activity toward E. coli, S. aureus and C. albicans and satisfied mechanical and water vapor barrier resistance. The coatings were also effective on reducing losses of weight and firmness of cheeses. Overall, antimicrobial nanoemulsion/edible films from renewable sources were successfully produced and showed high potential to be applicable in food industry to prolong the shelf-life of cheeses.",
publisher = "Sciences Invest",
journal = "Journal of International Scientific Publications Agriculture & Food",
title = "The use of nanoemulsion-based edible coatings to prolong the shelflife of cheese",
pages = "138-131",
volume = "5",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6247"
}

Miljković, M., Davidović, S., Davidović, S., Radovanović, N.,& Dimitrijević, S.. (2017). The use of nanoemulsion-based edible coatings to prolong the shelflife of cheese. in Journal of International Scientific Publications Agriculture & Food
Sciences Invest., 5, 131-138.
https://hdl.handle.net/21.15107/rcub_technorep_6247

Miljković M, Davidović S, Davidović S, Radovanović N, Dimitrijević S. The use of nanoemulsion-based edible coatings to prolong the shelflife of cheese. in Journal of International Scientific Publications Agriculture & Food. 2017;5:131-138.
https://hdl.handle.net/21.15107/rcub_technorep_6247 .

Miljković, Miona, Davidović, Slađana, Davidović, Slađana, Radovanović, Neda, Dimitrijević, Suzana, "The use of nanoemulsion-based edible coatings to prolong the shelflife of cheese" in Journal of International Scientific Publications Agriculture & Food, 5 (2017):131-138,
https://hdl.handle.net/21.15107/rcub_technorep_6247 .

TY  - CONF
AU  - Davidović, Slađana
AU  - Miljković, Miona
AU  - Radovanović, Neda
AU  - Lazic, Vesna
AU  - Nešić, Aleksandra
AU  - Dimitrijević, Suzana
PY  - 2016
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6182
AB  - Antimicrobial potential of a film made from dextran incorporated with silver nanoparticles (Ag NPs) against Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus was investigated. First, the colloidal Ag NPs solutions of three different concentrations: 0.5, 1.0 and 2.0 mmol L-1 were made. The next step was preparation of dextran/nanosilver films. Disc diffusion method on agar and quantitative test in liquid medium were performed to evaluate the antibacterial activity of prepared composites. The results obtained in this work demonstrated that dextran-based film incorporated with Ag NPs could be used as a coating to prevent contaminations of working surfaces.
C3  - NANO FOR HEALTH
T1  - Antimicrobial properties of dextran-based coatings incorporated with silver nanoparticles
SP  - 137
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6182
ER  - 

@conference{
author = "Davidović, Slađana and Miljković, Miona and Radovanović, Neda and Lazic, Vesna and Nešić, Aleksandra and Dimitrijević, Suzana",
year = "2016",
abstract = "Antimicrobial potential of a film made from dextran incorporated with silver nanoparticles (Ag NPs) against Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus was investigated. First, the colloidal Ag NPs solutions of three different concentrations: 0.5, 1.0 and 2.0 mmol L-1 were made. The next step was preparation of dextran/nanosilver films. Disc diffusion method on agar and quantitative test in liquid medium were performed to evaluate the antibacterial activity of prepared composites. The results obtained in this work demonstrated that dextran-based film incorporated with Ag NPs could be used as a coating to prevent contaminations of working surfaces.",
journal = "NANO FOR HEALTH",
title = "Antimicrobial properties of dextran-based coatings incorporated with silver nanoparticles",
pages = "137",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6182"
}

Davidović, S., Miljković, M., Radovanović, N., Lazic, V., Nešić, A.,& Dimitrijević, S.. (2016). Antimicrobial properties of dextran-based coatings incorporated with silver nanoparticles. in NANO FOR HEALTH, 137.
https://hdl.handle.net/21.15107/rcub_technorep_6182

Davidović S, Miljković M, Radovanović N, Lazic V, Nešić A, Dimitrijević S. Antimicrobial properties of dextran-based coatings incorporated with silver nanoparticles. in NANO FOR HEALTH. 2016;:137.
https://hdl.handle.net/21.15107/rcub_technorep_6182 .

Davidović, Slađana, Miljković, Miona, Radovanović, Neda, Lazic, Vesna, Nešić, Aleksandra, Dimitrijević, Suzana, "Antimicrobial properties of dextran-based coatings incorporated with silver nanoparticles" in NANO FOR HEALTH (2016):137,
https://hdl.handle.net/21.15107/rcub_technorep_6182 .

TY  - JOUR
AU  - Mihajlovski, Katarina
AU  - Davidović, Slađana
AU  - Simović, Milica
AU  - Radovanović, Neda
AU  - Šiler-Marinković, Slavica
AU  - Rajilić-Stojanović, Mirjana
AU  - Dimitrijević-Branković, Suzana
PY  - 2016
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3229
AB  - Cellulases are industrially important enzymes with a potential to convert cellulose into fermentable sugars. Novel bacterial isolate Paenibacillus sp. CKS1 was tested for cellulase activity and the optimal conditions for carboxymethyl cellulase (CMCase) production were determined. Maximum CMCase activity was obtained in the third passage of the bacterial culture after 3 days of incubation at 30 degrees C. Cellobiose and yeast extract was the optimal source of carbon and nitrogen for induction of CMCase activity. In addition, with initial pH 7 of the medium and 40 ml of working volume in 500 ml culture flasks with shaking at 150 rpm, the maximum CMCase activity in a crude culture supernatant reached value of 0.532 +/- 0.006 U/ml. For crude CMCase, optimal temperature was 50 degrees C and optimal pH 4.8, respectively. HPLC analysis confirmed the bacterium is capable to hydrolise CMC to glucose and other soluble sugars.
PB  - Savez hemijskih inženjera, Beograd
T2  - Hemijska industrija
T1  - Carboxymethyl cellulase production from a Paenibacillus sp.
EP  - 338
IS  - 3
SP  - 329
VL  - 70
DO  - 10.2298/HEMIND150222038M
ER  - 

@article{
author = "Mihajlovski, Katarina and Davidović, Slađana and Simović, Milica and Radovanović, Neda and Šiler-Marinković, Slavica and Rajilić-Stojanović, Mirjana and Dimitrijević-Branković, Suzana",
year = "2016",
abstract = "Cellulases are industrially important enzymes with a potential to convert cellulose into fermentable sugars. Novel bacterial isolate Paenibacillus sp. CKS1 was tested for cellulase activity and the optimal conditions for carboxymethyl cellulase (CMCase) production were determined. Maximum CMCase activity was obtained in the third passage of the bacterial culture after 3 days of incubation at 30 degrees C. Cellobiose and yeast extract was the optimal source of carbon and nitrogen for induction of CMCase activity. In addition, with initial pH 7 of the medium and 40 ml of working volume in 500 ml culture flasks with shaking at 150 rpm, the maximum CMCase activity in a crude culture supernatant reached value of 0.532 +/- 0.006 U/ml. For crude CMCase, optimal temperature was 50 degrees C and optimal pH 4.8, respectively. HPLC analysis confirmed the bacterium is capable to hydrolise CMC to glucose and other soluble sugars.",
publisher = "Savez hemijskih inženjera, Beograd",
journal = "Hemijska industrija",
title = "Carboxymethyl cellulase production from a Paenibacillus sp.",
pages = "338-329",
number = "3",
volume = "70",
doi = "10.2298/HEMIND150222038M"
}

Mihajlovski, K., Davidović, S., Simović, M., Radovanović, N., Šiler-Marinković, S., Rajilić-Stojanović, M.,& Dimitrijević-Branković, S.. (2016). Carboxymethyl cellulase production from a Paenibacillus sp.. in Hemijska industrija
Savez hemijskih inženjera, Beograd., 70(3), 329-338.
https://doi.org/10.2298/HEMIND150222038M

Mihajlovski K, Davidović S, Simović M, Radovanović N, Šiler-Marinković S, Rajilić-Stojanović M, Dimitrijević-Branković S. Carboxymethyl cellulase production from a Paenibacillus sp.. in Hemijska industrija. 2016;70(3):329-338.
doi:10.2298/HEMIND150222038M .

Mihajlovski, Katarina, Davidović, Slađana, Simović, Milica, Radovanović, Neda, Šiler-Marinković, Slavica, Rajilić-Stojanović, Mirjana, Dimitrijević-Branković, Suzana, "Carboxymethyl cellulase production from a Paenibacillus sp." in Hemijska industrija, 70, no. 3 (2016):329-338,
https://doi.org/10.2298/HEMIND150222038M . .

TY  - JOUR
AU  - Mihajlovski, Katarina
AU  - Radovanović, Neda
AU  - Veljović, Đorđe
AU  - Šiler-Marinković, Slavica
AU  - Dimitrijević-Branković, Suzana
PY  - 2016
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3390
AB  - Molasses and sugar beet pulp, by-products in sugar industry, are produced in large amounts annually which create disposal problems. High sugar content in sugar beet molasses enables its use for fermentations while sugar beet pulp represents an interesting cheap raw material source for enzyme production. beta-amylase production by a new strain Paenibacillus chitinolyticus CKS1 was performed using a molasses and sugar beet pulp. Two different pretreatments of SBP were investigated while scanning electron microscopy (SEM) showed immobilization of P. chitinolyticus CKS1 cells onto alkali treated SBP. The statistical model was design via Central Composite Design (CCD) under the Response Surface Methodology (RSM) using three variables (inoculum concentration, time of incubation and sugar beet pulp concentration) for obtaining maximum beta-amylase activity. An approximately 3.66-fold increase in beta-amylase production was achieved in the optimized medium as compared with the nonoptimized medium. A maximum beta-amylase production 2.237 U/ml was achieved under optimal conditions inoculum concentration 10%, incubation time 83.07 h and SBP concentration 3%. A high performance liquid chromatography showed that P. chitinolyticus CKS1 hydrolyzed starch to form maltose as a major product. These results indicate that beta-amylase production could be improved using these kind of processes. Also, taking into consideration cheap substrates, economic side of the process is justified.
PB  - Elsevier, Amsterdam
T2  - Industrial Crops and Products
T1  - Improved beta-amylase production on molasses and sugar beet pulp by a novel strain Paenibacillus chitinolyticus CKS1
EP  - 122
SP  - 115
VL  - 80
DO  - 10.1016/j.indcrop.2015.11.025
ER  - 

@article{
author = "Mihajlovski, Katarina and Radovanović, Neda and Veljović, Đorđe and Šiler-Marinković, Slavica and Dimitrijević-Branković, Suzana",
year = "2016",
abstract = "Molasses and sugar beet pulp, by-products in sugar industry, are produced in large amounts annually which create disposal problems. High sugar content in sugar beet molasses enables its use for fermentations while sugar beet pulp represents an interesting cheap raw material source for enzyme production. beta-amylase production by a new strain Paenibacillus chitinolyticus CKS1 was performed using a molasses and sugar beet pulp. Two different pretreatments of SBP were investigated while scanning electron microscopy (SEM) showed immobilization of P. chitinolyticus CKS1 cells onto alkali treated SBP. The statistical model was design via Central Composite Design (CCD) under the Response Surface Methodology (RSM) using three variables (inoculum concentration, time of incubation and sugar beet pulp concentration) for obtaining maximum beta-amylase activity. An approximately 3.66-fold increase in beta-amylase production was achieved in the optimized medium as compared with the nonoptimized medium. A maximum beta-amylase production 2.237 U/ml was achieved under optimal conditions inoculum concentration 10%, incubation time 83.07 h and SBP concentration 3%. A high performance liquid chromatography showed that P. chitinolyticus CKS1 hydrolyzed starch to form maltose as a major product. These results indicate that beta-amylase production could be improved using these kind of processes. Also, taking into consideration cheap substrates, economic side of the process is justified.",
publisher = "Elsevier, Amsterdam",
journal = "Industrial Crops and Products",
title = "Improved beta-amylase production on molasses and sugar beet pulp by a novel strain Paenibacillus chitinolyticus CKS1",
pages = "122-115",
volume = "80",
doi = "10.1016/j.indcrop.2015.11.025"
}

Mihajlovski, K., Radovanović, N., Veljović, Đ., Šiler-Marinković, S.,& Dimitrijević-Branković, S.. (2016). Improved beta-amylase production on molasses and sugar beet pulp by a novel strain Paenibacillus chitinolyticus CKS1. in Industrial Crops and Products
Elsevier, Amsterdam., 80, 115-122.
https://doi.org/10.1016/j.indcrop.2015.11.025

Mihajlovski K, Radovanović N, Veljović Đ, Šiler-Marinković S, Dimitrijević-Branković S. Improved beta-amylase production on molasses and sugar beet pulp by a novel strain Paenibacillus chitinolyticus CKS1. in Industrial Crops and Products. 2016;80:115-122.
doi:10.1016/j.indcrop.2015.11.025 .

Mihajlovski, Katarina, Radovanović, Neda, Veljović, Đorđe, Šiler-Marinković, Slavica, Dimitrijević-Branković, Suzana, "Improved beta-amylase production on molasses and sugar beet pulp by a novel strain Paenibacillus chitinolyticus CKS1" in Industrial Crops and Products, 80 (2016):115-122,
https://doi.org/10.1016/j.indcrop.2015.11.025 . .

TY  - CONF
AU  - Mihajlovski, Katarina R.
AU  - Radovanović, Neda R.
AU  - Miljković, Miona M.
AU  - Mladenović, Dragana D.
AU  - Dimitrijević-Branković, Suzana I.
AU  - Šiler-Marinković, Slavica
PY  - 2015
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6159
AB  - Sugar beet pulp (SBP) and molasses, by-products from sugar industry were used as a substrate for cellulase production in this study. Solid state fermentation (SSF) was performed by a natural isolate Paenibacillus chitinolyticus CKS1. Data showed that cellulose in SBP could be used as a substrate to produce both cellulases, CMCase and Avicelase. An optimum solid: moisture ratio for maximum cellulase production was investigated. Maximum CMCase 3.159 U/g and Avicelase activity 4.840 U/g was obtained at the fourth day of incubation with 10% of inoculum. The cellulase production during SSF on SBP indicates that this process is economically very justified.
PB  - Bor : Technical Faculty
C3  - Proceedings / XXIII International Conference "Ecological Truth", Eco-Ist '15, 17-20 June 2015, Kopaonik, Serbia
T1  - Sugar beet pulp and molasses as a solid state fermentation media for cellulase production by Paenibacillus chitinolyticus CKS1
EP  - 408
SP  - 403
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6159
ER  - 

@conference{
author = "Mihajlovski, Katarina R. and Radovanović, Neda R. and Miljković, Miona M. and Mladenović, Dragana D. and Dimitrijević-Branković, Suzana I. and Šiler-Marinković, Slavica",
year = "2015",
abstract = "Sugar beet pulp (SBP) and molasses, by-products from sugar industry were used as a substrate for cellulase production in this study. Solid state fermentation (SSF) was performed by a natural isolate Paenibacillus chitinolyticus CKS1. Data showed that cellulose in SBP could be used as a substrate to produce both cellulases, CMCase and Avicelase. An optimum solid: moisture ratio for maximum cellulase production was investigated. Maximum CMCase 3.159 U/g and Avicelase activity 4.840 U/g was obtained at the fourth day of incubation with 10% of inoculum. The cellulase production during SSF on SBP indicates that this process is economically very justified.",
publisher = "Bor : Technical Faculty",
journal = "Proceedings / XXIII International Conference "Ecological Truth", Eco-Ist '15, 17-20 June 2015, Kopaonik, Serbia",
title = "Sugar beet pulp and molasses as a solid state fermentation media for cellulase production by Paenibacillus chitinolyticus CKS1",
pages = "408-403",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6159"
}

Mihajlovski, K. R., Radovanović, N. R., Miljković, M. M., Mladenović, D. D., Dimitrijević-Branković, S. I.,& Šiler-Marinković, S.. (2015). Sugar beet pulp and molasses as a solid state fermentation media for cellulase production by Paenibacillus chitinolyticus CKS1. in Proceedings / XXIII International Conference "Ecological Truth", Eco-Ist '15, 17-20 June 2015, Kopaonik, Serbia
Bor : Technical Faculty., 403-408.
https://hdl.handle.net/21.15107/rcub_technorep_6159

Mihajlovski KR, Radovanović NR, Miljković MM, Mladenović DD, Dimitrijević-Branković SI, Šiler-Marinković S. Sugar beet pulp and molasses as a solid state fermentation media for cellulase production by Paenibacillus chitinolyticus CKS1. in Proceedings / XXIII International Conference "Ecological Truth", Eco-Ist '15, 17-20 June 2015, Kopaonik, Serbia. 2015;:403-408.
https://hdl.handle.net/21.15107/rcub_technorep_6159 .

Mihajlovski, Katarina R., Radovanović, Neda R., Miljković, Miona M., Mladenović, Dragana D., Dimitrijević-Branković, Suzana I., Šiler-Marinković, Slavica, "Sugar beet pulp and molasses as a solid state fermentation media for cellulase production by Paenibacillus chitinolyticus CKS1" in Proceedings / XXIII International Conference "Ecological Truth", Eco-Ist '15, 17-20 June 2015, Kopaonik, Serbia (2015):403-408,
https://hdl.handle.net/21.15107/rcub_technorep_6159 .

TY  - JOUR
AU  - Mihajlovski, Katarina
AU  - Radovanović, Neda
AU  - Miljković, Miona
AU  - Šiler-Marinković, Slavica
AU  - Rajilić-Stojanović, Mirjana
AU  - Dimitrijević-Branković, Suzana
PY  - 2015
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3069
AB  - Transport packaging factories generate a large amount of wastewater that contains residuals of starch glue. These residuals could be used as substrates for microorganism growth and enzyme production. In this study, beta-amylase production using a new strain Paenibacillus chitinolyticus CKS1 was optimized using wastewater from a Serbian transport packaging factory. The optimization of beta-amylase production was carried out using Response Surface Methodology (RSM). A Central Composite Design under the RSM with four interacting parameters (incubation time, inoculum concentration, casein hydrolysate concentration and yeast extract concentration) was employed to identify the optimal conditions for maximum beta-amylase activity (334.20 U L-1) as 62 h of incubation with 2.40% inoculum, 2.02 g L-1 casein hydrolysate and 3.98 g L-1 yeast extract. High performance liquid chromatography showed that the P. chitinolyticus CKS1 strain hydrolyzed starch to form maltose as a major product. Due to the application of wastewater as an inexpensive material for enzyme and maltose production it may be considered that the economic and eco-friendly aspects of this method are very promising.
PB  - Royal Society of Chemistry
T2  - RSC Advances
T1  - beta-Amylase production from packaging-industry wastewater using a novel strain Paenibacillus chitinolyticus CKS 1
EP  - 90903
IS  - 110
SP  - 90895
VL  - 5
DO  - 10.1039/c5ra11964b
ER  - 

@article{
author = "Mihajlovski, Katarina and Radovanović, Neda and Miljković, Miona and Šiler-Marinković, Slavica and Rajilić-Stojanović, Mirjana and Dimitrijević-Branković, Suzana",
year = "2015",
abstract = "Transport packaging factories generate a large amount of wastewater that contains residuals of starch glue. These residuals could be used as substrates for microorganism growth and enzyme production. In this study, beta-amylase production using a new strain Paenibacillus chitinolyticus CKS1 was optimized using wastewater from a Serbian transport packaging factory. The optimization of beta-amylase production was carried out using Response Surface Methodology (RSM). A Central Composite Design under the RSM with four interacting parameters (incubation time, inoculum concentration, casein hydrolysate concentration and yeast extract concentration) was employed to identify the optimal conditions for maximum beta-amylase activity (334.20 U L-1) as 62 h of incubation with 2.40% inoculum, 2.02 g L-1 casein hydrolysate and 3.98 g L-1 yeast extract. High performance liquid chromatography showed that the P. chitinolyticus CKS1 strain hydrolyzed starch to form maltose as a major product. Due to the application of wastewater as an inexpensive material for enzyme and maltose production it may be considered that the economic and eco-friendly aspects of this method are very promising.",
publisher = "Royal Society of Chemistry",
journal = "RSC Advances",
title = "beta-Amylase production from packaging-industry wastewater using a novel strain Paenibacillus chitinolyticus CKS 1",
pages = "90903-90895",
number = "110",
volume = "5",
doi = "10.1039/c5ra11964b"
}

Mihajlovski, K., Radovanović, N., Miljković, M., Šiler-Marinković, S., Rajilić-Stojanović, M.,& Dimitrijević-Branković, S.. (2015). beta-Amylase production from packaging-industry wastewater using a novel strain Paenibacillus chitinolyticus CKS 1. in RSC Advances
Royal Society of Chemistry., 5(110), 90895-90903.
https://doi.org/10.1039/c5ra11964b

Mihajlovski K, Radovanović N, Miljković M, Šiler-Marinković S, Rajilić-Stojanović M, Dimitrijević-Branković S. beta-Amylase production from packaging-industry wastewater using a novel strain Paenibacillus chitinolyticus CKS 1. in RSC Advances. 2015;5(110):90895-90903.
doi:10.1039/c5ra11964b .

Mihajlovski, Katarina, Radovanović, Neda, Miljković, Miona, Šiler-Marinković, Slavica, Rajilić-Stojanović, Mirjana, Dimitrijević-Branković, Suzana, "beta-Amylase production from packaging-industry wastewater using a novel strain Paenibacillus chitinolyticus CKS 1" in RSC Advances, 5, no. 110 (2015):90895-90903,
https://doi.org/10.1039/c5ra11964b . .

TY  - JOUR
AU  - Milutinović, Milica
AU  - Radovanović, Neda
AU  - Ćorović, Marija
AU  - Šiler-Marinković, Slavica
AU  - Rajilić-Stojanović, Mirjana
AU  - Dimitrijević-Branković, Suzana
PY  - 2015
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3098
AB  - Microwave-assisted extraction of polyphenols and flavonoids from waste Achillea millefolium (yarrow) dust was optimised using the response surface methodology. The influence of the extraction time (13-47 s), ethanol concentration (53-87%), liquid/solid ratio (13-47 mL/g) and microwave power (170 and 340W) was studied. The optimised conditions for the maximal recovery of polyphenols and flavonoids were as follows: 33 s of extraction time, 70% of ethanol concentration, 40 mL/g of liquid/solid ratio and 170W of microwave power. Under the optimal conditions, 237.74 +/- 2.08 mg of gallic acid equivalents per g of extract dry matter of total polyphenols, and 42.95 +/- 1.32 mg of quercetin equivalents per g of extract dry matter of total flavonoids were extracted. The optimal extract strongly reduced the diphenylpicrylhydrazyl radical (IC50 7.89 mu g/mL). The antioxidant activity of the obtained extracts was in correlation to the total polyphenol content in yarrow extracts. Compared to the conventional extractions (solid-liquid extraction (24 h) and maceration (48 h)) microwave-assisted extraction yielded extracts with higher polyphenol and flavonoid content and higher antioxidant activity in very short time (33 s).
PB  - Elsevier Science Bv, Amsterdam
T2  - Industrial Crops and Products
T1  - Optimisation of microwave-assisted extraction parameters for antioxidants from waste Achillea millefolium dust
EP  - 341
SP  - 333
VL  - 77
DO  - 10.1016/j.indcrop.2015.09.007
ER  - 

@article{
author = "Milutinović, Milica and Radovanović, Neda and Ćorović, Marija and Šiler-Marinković, Slavica and Rajilić-Stojanović, Mirjana and Dimitrijević-Branković, Suzana",
year = "2015",
abstract = "Microwave-assisted extraction of polyphenols and flavonoids from waste Achillea millefolium (yarrow) dust was optimised using the response surface methodology. The influence of the extraction time (13-47 s), ethanol concentration (53-87%), liquid/solid ratio (13-47 mL/g) and microwave power (170 and 340W) was studied. The optimised conditions for the maximal recovery of polyphenols and flavonoids were as follows: 33 s of extraction time, 70% of ethanol concentration, 40 mL/g of liquid/solid ratio and 170W of microwave power. Under the optimal conditions, 237.74 +/- 2.08 mg of gallic acid equivalents per g of extract dry matter of total polyphenols, and 42.95 +/- 1.32 mg of quercetin equivalents per g of extract dry matter of total flavonoids were extracted. The optimal extract strongly reduced the diphenylpicrylhydrazyl radical (IC50 7.89 mu g/mL). The antioxidant activity of the obtained extracts was in correlation to the total polyphenol content in yarrow extracts. Compared to the conventional extractions (solid-liquid extraction (24 h) and maceration (48 h)) microwave-assisted extraction yielded extracts with higher polyphenol and flavonoid content and higher antioxidant activity in very short time (33 s).",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Industrial Crops and Products",
title = "Optimisation of microwave-assisted extraction parameters for antioxidants from waste Achillea millefolium dust",
pages = "341-333",
volume = "77",
doi = "10.1016/j.indcrop.2015.09.007"
}

Milutinović, M., Radovanović, N., Ćorović, M., Šiler-Marinković, S., Rajilić-Stojanović, M.,& Dimitrijević-Branković, S.. (2015). Optimisation of microwave-assisted extraction parameters for antioxidants from waste Achillea millefolium dust. in Industrial Crops and Products
Elsevier Science Bv, Amsterdam., 77, 333-341.
https://doi.org/10.1016/j.indcrop.2015.09.007

Milutinović M, Radovanović N, Ćorović M, Šiler-Marinković S, Rajilić-Stojanović M, Dimitrijević-Branković S. Optimisation of microwave-assisted extraction parameters for antioxidants from waste Achillea millefolium dust. in Industrial Crops and Products. 2015;77:333-341.
doi:10.1016/j.indcrop.2015.09.007 .

Milutinović, Milica, Radovanović, Neda, Ćorović, Marija, Šiler-Marinković, Slavica, Rajilić-Stojanović, Mirjana, Dimitrijević-Branković, Suzana, "Optimisation of microwave-assisted extraction parameters for antioxidants from waste Achillea millefolium dust" in Industrial Crops and Products, 77 (2015):333-341,
https://doi.org/10.1016/j.indcrop.2015.09.007 . .

TY  - CONF
AU  - Pavlović, Marija
AU  - Buntić, Aneta
AU  - Šiler-Marinković, Slavica
AU  - Antonović, Dušan
AU  - Milutinović, Milica
AU  - Radovanović, Neda
AU  - Dimitrijević-Branković, Suzana
PY  - 2014
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6427
AB  - Spent coffee grounds were evaluated for adsorption
efficiency in the removal of pesticide paraquat from its aqueous
solutions. The influence of the adsorbent mass, agitation time,
paraquat concentration and pH have been investigated in the batch
sorption mode. Adsorption capacity (mg/g) has been found to be
27.87 ± 1.57 mg/g, while the removal efficiency has resulted of 32.25
± 1.79 %. Adsorption rate was found to be very fast, whereby the
time required for equilibrium adsorption occurred within first 20
minutes. The process behavior was predicted using the second-order
kinetic model. On the basis of the observed data, further process
optimization may be greatly facilitated, thus it is considered that
coffee waste may be exploited well for pesticides removal purposes.
PB  - International Institute of Chemical, Biological and Environmental Engineering, Kuala Lumpur, Malaysia
C3  - International conference on civil, biological and environmental engineering (CBEE)
T1  - Spent coffee grounds as adsorbents for pesticide paraquat removal from its aqueous solutions
EP  - 65
SP  - 60
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6427
ER  - 

@conference{
author = "Pavlović, Marija and Buntić, Aneta and Šiler-Marinković, Slavica and Antonović, Dušan and Milutinović, Milica and Radovanović, Neda and Dimitrijević-Branković, Suzana",
year = "2014",
abstract = "Spent coffee grounds were evaluated for adsorption
efficiency in the removal of pesticide paraquat from its aqueous
solutions. The influence of the adsorbent mass, agitation time,
paraquat concentration and pH have been investigated in the batch
sorption mode. Adsorption capacity (mg/g) has been found to be
27.87 ± 1.57 mg/g, while the removal efficiency has resulted of 32.25
± 1.79 %. Adsorption rate was found to be very fast, whereby the
time required for equilibrium adsorption occurred within first 20
minutes. The process behavior was predicted using the second-order
kinetic model. On the basis of the observed data, further process
optimization may be greatly facilitated, thus it is considered that
coffee waste may be exploited well for pesticides removal purposes.",
publisher = "International Institute of Chemical, Biological and Environmental Engineering, Kuala Lumpur, Malaysia",
journal = "International conference on civil, biological and environmental engineering (CBEE)",
title = "Spent coffee grounds as adsorbents for pesticide paraquat removal from its aqueous solutions",
pages = "65-60",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6427"
}

Pavlović, M., Buntić, A., Šiler-Marinković, S., Antonović, D., Milutinović, M., Radovanović, N.,& Dimitrijević-Branković, S.. (2014). Spent coffee grounds as adsorbents for pesticide paraquat removal from its aqueous solutions. in International conference on civil, biological and environmental engineering (CBEE)
International Institute of Chemical, Biological and Environmental Engineering, Kuala Lumpur, Malaysia., 60-65.
https://hdl.handle.net/21.15107/rcub_technorep_6427

Pavlović M, Buntić A, Šiler-Marinković S, Antonović D, Milutinović M, Radovanović N, Dimitrijević-Branković S. Spent coffee grounds as adsorbents for pesticide paraquat removal from its aqueous solutions. in International conference on civil, biological and environmental engineering (CBEE). 2014;:60-65.
https://hdl.handle.net/21.15107/rcub_technorep_6427 .

Pavlović, Marija, Buntić, Aneta, Šiler-Marinković, Slavica, Antonović, Dušan, Milutinović, Milica, Radovanović, Neda, Dimitrijević-Branković, Suzana, "Spent coffee grounds as adsorbents for pesticide paraquat removal from its aqueous solutions" in International conference on civil, biological and environmental engineering (CBEE) (2014):60-65,
https://hdl.handle.net/21.15107/rcub_technorep_6427 .

TY  - JOUR
AU  - Milutinović, Milica
AU  - Radovanović, Neda
AU  - Rajilić-Stojanović, Mirjana
AU  - Šiler-Marinković, Slavica
AU  - Dimitrijević, Snežana
AU  - Dimitrijević-Branković, Suzana
PY  - 2014
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2736
AB  - Microwave-assisted extraction was optimised by the response surface methodology in order to enhance the extraction of polyphenols from Equisetum arvense (horsetail). Under the optimal conditions the total polyphenol content in extract reached a concentration of 161.57 mg of gallic acid equivalents per g of extract dry matter. The optimal conditions were reached with 54.5% of ethanol, 45.1 mL/g liquid/solid ratio, the microwave power of 170 Wand 80s extraction time. The antioxidant activity of obtained extract was evaluated by FRAP (ferric reducing antioxidant power) and DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging activity assay. Since, the conditions that favoured maximum polyphenol content in extract were the same as the conditions that favoured maximum antioxidant activity, the polyphenols were identified as the predominant antioxidants in horsetail extracts. When compared to the conventional extraction method (solid liquid extraction for 12 h), our results showed that more polyphenols can be extracted from waste horsetail using microwave extraction and aqueous ethanol, for just 80s. The extracts obtained by optimised extraction procedure exhibit significant antioxidant activity and can be used as a natural source of antioxidants.
PB  - Elsevier, Amsterdam
T2  - Industrial Crops and Products
T1  - Microwave-assisted extraction for the recovery of antioxidants from waste Equisetum arvense
EP  - 397
SP  - 388
VL  - 61
DO  - 10.1016/j.indcrop.2014.07.039
ER  - 

@article{
author = "Milutinović, Milica and Radovanović, Neda and Rajilić-Stojanović, Mirjana and Šiler-Marinković, Slavica and Dimitrijević, Snežana and Dimitrijević-Branković, Suzana",
year = "2014",
abstract = "Microwave-assisted extraction was optimised by the response surface methodology in order to enhance the extraction of polyphenols from Equisetum arvense (horsetail). Under the optimal conditions the total polyphenol content in extract reached a concentration of 161.57 mg of gallic acid equivalents per g of extract dry matter. The optimal conditions were reached with 54.5% of ethanol, 45.1 mL/g liquid/solid ratio, the microwave power of 170 Wand 80s extraction time. The antioxidant activity of obtained extract was evaluated by FRAP (ferric reducing antioxidant power) and DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging activity assay. Since, the conditions that favoured maximum polyphenol content in extract were the same as the conditions that favoured maximum antioxidant activity, the polyphenols were identified as the predominant antioxidants in horsetail extracts. When compared to the conventional extraction method (solid liquid extraction for 12 h), our results showed that more polyphenols can be extracted from waste horsetail using microwave extraction and aqueous ethanol, for just 80s. The extracts obtained by optimised extraction procedure exhibit significant antioxidant activity and can be used as a natural source of antioxidants.",
publisher = "Elsevier, Amsterdam",
journal = "Industrial Crops and Products",
title = "Microwave-assisted extraction for the recovery of antioxidants from waste Equisetum arvense",
pages = "397-388",
volume = "61",
doi = "10.1016/j.indcrop.2014.07.039"
}

Milutinović, M., Radovanović, N., Rajilić-Stojanović, M., Šiler-Marinković, S., Dimitrijević, S.,& Dimitrijević-Branković, S.. (2014). Microwave-assisted extraction for the recovery of antioxidants from waste Equisetum arvense. in Industrial Crops and Products
Elsevier, Amsterdam., 61, 388-397.
https://doi.org/10.1016/j.indcrop.2014.07.039

Milutinović M, Radovanović N, Rajilić-Stojanović M, Šiler-Marinković S, Dimitrijević S, Dimitrijević-Branković S. Microwave-assisted extraction for the recovery of antioxidants from waste Equisetum arvense. in Industrial Crops and Products. 2014;61:388-397.
doi:10.1016/j.indcrop.2014.07.039 .

Milutinović, Milica, Radovanović, Neda, Rajilić-Stojanović, Mirjana, Šiler-Marinković, Slavica, Dimitrijević, Snežana, Dimitrijević-Branković, Suzana, "Microwave-assisted extraction for the recovery of antioxidants from waste Equisetum arvense" in Industrial Crops and Products, 61 (2014):388-397,
https://doi.org/10.1016/j.indcrop.2014.07.039 . .